Rnf144b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf144b
Entrez Gene ID	218215
Full Name	ring finger protein 144B
Synonyms	BC025007,E130105P19Rik,Ibrdc2,Pir2
General protein information	Preferred Names ring finger protein 144B Names E3 ubiquitin-protein ligase RNF144B IBR domain containing 2 IBR domain-containing protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170643.1	NP_001164114.1	E3 ubiquitin-protein ligase RNF144B
NM_146042.4	NP_666154.3	E3 ubiquitin-protein ligase RNF144B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNF144B	XP_518261.3
	RNF144B	XP_001097834.1
Mus musculus (house mouse)	Rnf144b	NP_001164114.1
Gallus gallus (chicken)	RNF144B	XP_004939680.1
Danio rerio (zebrafish)	zgc:63907	NP_957431.2
Xenopus tropicalis (tropical clawed frog)	rnf144b	NP_001107142.1
Bos taurus (cattle)	RNF144B	NP_001092498.1
Homo sapiens (human)	RNF144B	NP_877434.2
Canis lupus familiaris (dog)	RNF144B	XP_005640120.1
Rattus norvegicus (Norway rat)	Rnf144b	NP_001102351.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Miller JA, Nathanson J, Franjic D, Shim S, Dalley RA, Shapouri S, Smith KA, Sunkin SM, Bernard A, Bennett JL, Lee CK, Hawrylycz MJ, Jones AR, Amaral DG, ?estan N, Gage FH, Lein ES
Development (Cambridge, England)140(22)4633-44(2013 Nov)

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Shalom-Barak T, Zhang X, Chu T, Timothy Schaiff W, Reddy JK, Xu J, Sadovsky Y, Barak Y
Developmental biology372(1)143-55(2012 Dec)

Differential control of TAp73 and DeltaNp73 protein stability by the ring finger ubiquitin ligase PIR2.
Sayan BS, Yang AL, Conforti F, Tucci P, Piro MC, Browne GJ, Agostini M, Bernardini S, Knight RA, Mak TW, Melino G
Proceedings of the National Academy of Sciences of the United States of America107(29)12877-82(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PIR2, by being induced by TAp73 and degrading DeltaNp73, differentially regulates TAp73/DeltaNp73 stability.[PIR2]
Title: Differential control of TAp73 and DeltaNp73 protein stability by the ring finger ubiquitin ligase PIR2.

The following Rnf144b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf144b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11638	NM_146042.4 Latest version!	Mus musculus ring finger protein 144B (Rnf144b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$146.30 $209.00
OMu11638	NM_001170643.1 Latest version!	Mus musculus ring finger protein 144B (Rnf144b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$146.30 $209.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf144b ( NM_001170643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164114.1 Mus musculus ring finger protein 144B (Rnf144b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11638
Clone ID Related Accession (Same CDS sequence)	NM_001170643.1 , NM_146042.4
Accession Version	NM_001170643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF144B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB661852.1, AK040939.1 and AC131085.16. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.36955.1, SRR1660813.98855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCCG TCGATGGGCT CCAGTGTCTG ACCATGACTG CGGAAAACCC ACCCTCTGGA 
GACCTGATTC CCGCCCCGCT CGTCACTTGC AAGCTCTGCC TGTGTGAACA GTCTCTGGAC 
AAGATGACCA TGCTCCAAGA GTGCCAGTGT ATCTTTTGTA CACCCTGCCT GAAACAGTAT 
ATGGTGCTGT CAATCCGAGA AGGCTGTGGA TCTCCTATCA CTTGTCCTGA CATGGTGTGC 
CTAAACCACG GGACCCTGCA GGAAACCGAG ATTGCCTGTT TGGTGCCTCT GGATGAGTTT 
CAGCTGTATC AGCGGCTCAA GTTTGAACGA GAGGTTCACA TGGACCCCCT CCGGACATGG 
TGTCCTGTTG CAGACTGCCA GACCGTGTGC CATATTTCAG CCGGAGACCC AGGCCAGCCA 
GTGTTGGTGG AGTGCCCTTC TTGCCACCTG AAATTCTGCT CATGTTGCAA GGATGCTTGG 
CACGAGGAGT CTTCCTGTAG AGACAGCCAG TCGGCGATGC CAGAGCATGG GGCTCTCTTT 
GGAACGGATG CGGACGCCCC CATCAAGCAG TGCCCGGTTT GCCGCATTTA CATTGAGCGC 
AACGAAGGCT GCGCCCAGAT GATGTGCAAG AACTGCAAGC ACACATTTTG CTGGTACTGT 
CTGCAGAACC TGGACAATGA CATATTCCTC AGGCACTATG ACAAAGGGCC ATGCAGGAAT 
AAGCTCGGCC ACTCGAGGGC ATCGGTAATG TGGAACCGAA CACAGGTAGT GGGGATCCTT 
GTGGGATTAG GTGTCATTGC CTTGGTGACC TCACCCTTGC TGCTCCTGGC CTCTCCCTGT 
ATAATCTGCT GTGTCTGCAA GTCCTGTCGC GGCAAGAAGA AAAAGCATGA TCCATCCACA 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164114.1
CDS	145..1050
Translation	MDSVDGLQCLTMTAENPPSGDLIPAPLVTCKLCLCEQSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCLNHGTLQETEIACLVPLDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECPSCHLKFCSCCKDAWHEESSCRDSQSAMPEHGALFGTDADAPIKQCPVCRIYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPCRNKLGHSRASVMWNRTQVVGILVGLGVIALVTSPLLLLASPCIICCVCKSCRGKKKKHDPSTT

Target ORF information:

RefSeq Version	NM_001170643.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 144B (Rnf144b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170643.1

ORF Insert Sequence:

ATGGACTCCG TCGATGGGCT CCAGTGTCTG ACCATGACTG CGGAAAACCC ACCCTCTGGA 
GACCTGATTC CCGCCCCGCT CGTCACTTGC AAGCTCTGCC TGTGTGAACA GTCTCTGGAC 
AAGATGACCA TGCTCCAAGA GTGCCAGTGT ATCTTTTGTA CACCCTGCCT GAAACAGTAT 
ATGGTGCTGT CAATCCGAGA AGGCTGTGGA TCTCCTATCA CTTGTCCTGA CATGGTGTGC 
CTAAACCACG GGACCCTGCA GGAAACCGAG ATTGCCTGTT TGGTGCCTCT GGATGAGTTT 
CAGCTGTATC AGCGGCTCAA GTTTGAACGA GAGGTTCACA TGGACCCCCT CCGGACATGG 
TGTCCTGTTG CAGACTGCCA GACCGTGTGC CATATTTCAG CCGGAGACCC AGGCCAGCCA 
GTGTTGGTGG AGTGCCCTTC TTGCCACCTG AAATTCTGCT CATGTTGCAA GGATGCTTGG 
CACGAGGAGT CTTCCTGTAG AGACAGCCAG TCGGCGATGC CAGAGCATGG GGCTCTCTTT 
GGAACGGATG CGGACGCCCC CATCAAGCAG TGCCCGGTTT GCCGCATTTA CATTGAGCGC 
AACGAAGGCT GCGCCCAGAT GATGTGCAAG AACTGCAAGC ACACATTTTG CTGGTACTGT 
CTGCAGAACC TGGACAATGA CATATTCCTC AGGCACTATG ACAAAGGGCC ATGCAGGAAT 
AAGCTCGGCC ACTCGAGGGC ATCGGTAATG TGGAACCGAA CACAGGTAGT GGGGATCCTT 
GTGGGATTAG GTGTCATTGC CTTGGTGACC TCACCCTTGC TGCTCCTGGC CTCTCCCTGT 
ATAATCTGCT GTGTCTGCAA GTCCTGTCGC GGCAAGAAGA AAAAGCATGA TCCATCCACA 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf144b ( NM_146042.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666154.3 Mus musculus ring finger protein 144B (Rnf144b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11638
Clone ID Related Accession (Same CDS sequence)	NM_001170643.1 , NM_146042.4
Accession Version	NM_146042.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF144B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053529.1, AK040939.1 and AC131085.16. On Dec 29, 2009 this sequence version replaced NM_146042.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK053529.1, SRR1660819.83909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCCG TCGATGGGCT CCAGTGTCTG ACCATGACTG CGGAAAACCC ACCCTCTGGA 
GACCTGATTC CCGCCCCGCT CGTCACTTGC AAGCTCTGCC TGTGTGAACA GTCTCTGGAC 
AAGATGACCA TGCTCCAAGA GTGCCAGTGT ATCTTTTGTA CACCCTGCCT GAAACAGTAT 
ATGGTGCTGT CAATCCGAGA AGGCTGTGGA TCTCCTATCA CTTGTCCTGA CATGGTGTGC 
CTAAACCACG GGACCCTGCA GGAAACCGAG ATTGCCTGTT TGGTGCCTCT GGATGAGTTT 
CAGCTGTATC AGCGGCTCAA GTTTGAACGA GAGGTTCACA TGGACCCCCT CCGGACATGG 
TGTCCTGTTG CAGACTGCCA GACCGTGTGC CATATTTCAG CCGGAGACCC AGGCCAGCCA 
GTGTTGGTGG AGTGCCCTTC TTGCCACCTG AAATTCTGCT CATGTTGCAA GGATGCTTGG 
CACGAGGAGT CTTCCTGTAG AGACAGCCAG TCGGCGATGC CAGAGCATGG GGCTCTCTTT 
GGAACGGATG CGGACGCCCC CATCAAGCAG TGCCCGGTTT GCCGCATTTA CATTGAGCGC 
AACGAAGGCT GCGCCCAGAT GATGTGCAAG AACTGCAAGC ACACATTTTG CTGGTACTGT 
CTGCAGAACC TGGACAATGA CATATTCCTC AGGCACTATG ACAAAGGGCC ATGCAGGAAT 
AAGCTCGGCC ACTCGAGGGC ATCGGTAATG TGGAACCGAA CACAGGTAGT GGGGATCCTT 
GTGGGATTAG GTGTCATTGC CTTGGTGACC TCACCCTTGC TGCTCCTGGC CTCTCCCTGT 
ATAATCTGCT GTGTCTGCAA GTCCTGTCGC GGCAAGAAGA AAAAGCATGA TCCATCCACA 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666154.3
CDS	181..1086
Translation	MDSVDGLQCLTMTAENPPSGDLIPAPLVTCKLCLCEQSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCLNHGTLQETEIACLVPLDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECPSCHLKFCSCCKDAWHEESSCRDSQSAMPEHGALFGTDADAPIKQCPVCRIYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPCRNKLGHSRASVMWNRTQVVGILVGLGVIALVTSPLLLLASPCIICCVCKSCRGKKKKHDPSTT

Target ORF information:

RefSeq Version	NM_146042.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 144B (Rnf144b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146042.4

ORF Insert Sequence:

ATGGACTCCG TCGATGGGCT CCAGTGTCTG ACCATGACTG CGGAAAACCC ACCCTCTGGA 
GACCTGATTC CCGCCCCGCT CGTCACTTGC AAGCTCTGCC TGTGTGAACA GTCTCTGGAC 
AAGATGACCA TGCTCCAAGA GTGCCAGTGT ATCTTTTGTA CACCCTGCCT GAAACAGTAT 
ATGGTGCTGT CAATCCGAGA AGGCTGTGGA TCTCCTATCA CTTGTCCTGA CATGGTGTGC 
CTAAACCACG GGACCCTGCA GGAAACCGAG ATTGCCTGTT TGGTGCCTCT GGATGAGTTT 
CAGCTGTATC AGCGGCTCAA GTTTGAACGA GAGGTTCACA TGGACCCCCT CCGGACATGG 
TGTCCTGTTG CAGACTGCCA GACCGTGTGC CATATTTCAG CCGGAGACCC AGGCCAGCCA 
GTGTTGGTGG AGTGCCCTTC TTGCCACCTG AAATTCTGCT CATGTTGCAA GGATGCTTGG 
CACGAGGAGT CTTCCTGTAG AGACAGCCAG TCGGCGATGC CAGAGCATGG GGCTCTCTTT 
GGAACGGATG CGGACGCCCC CATCAAGCAG TGCCCGGTTT GCCGCATTTA CATTGAGCGC 
AACGAAGGCT GCGCCCAGAT GATGTGCAAG AACTGCAAGC ACACATTTTG CTGGTACTGT 
CTGCAGAACC TGGACAATGA CATATTCCTC AGGCACTATG ACAAAGGGCC ATGCAGGAAT 
AAGCTCGGCC ACTCGAGGGC ATCGGTAATG TGGAACCGAA CACAGGTAGT GGGGATCCTT 
GTGGGATTAG GTGTCATTGC CTTGGTGACC TCACCCTTGC TGCTCCTGGC CTCTCCCTGT 
ATAATCTGCT GTGTCTGCAA GTCCTGTCGC GGCAAGAAGA AAAAGCATGA TCCATCCACA 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Development (Cambridge, England)140(22)4633-44(2013 Nov)
Miller JA,Nathanson J,Franjic D,Shim S,Dalley RA,Shapouri S,Smith KA,Sunkin SM,Bernard A,Bennett JL,Lee CK,Hawrylycz MJ,Jones AR,Amaral DG,?estan N,Gage FH,Lein ES

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Developmental biology372(1)143-55(2012 Dec)
Shalom-Barak T,Zhang X,Chu T,Timothy Schaiff W,Reddy JK,Xu J,Sadovsky Y,Barak Y

Differential control of TAp73 and DeltaNp73 protein stability by the ring finger ubiquitin ligase PIR2.
Proceedings of the National Academy of Sciences of the United States of America107(29)12877-82(2010 Jul)
Sayan BS,Yang AL,Conforti F,Tucci P,Piro MC,Browne GJ,Agostini M,Bernardini S,Knight RA,Mak TW,Melino G

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Rnf144b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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