Tgfb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tgfb3
Entrez Gene ID	21809
Full Name	transforming growth factor, beta 3
Synonyms	TGF-beta-3,Tgfb-3
General protein information	Preferred Names transforming growth factor, beta 3 Names transforming growth factor beta-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 D2\|12 40.09 cM
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This protein is involved in embryogenesis and cell differentiation, and may play a role in wound healing. Homozygous knockout mice for this gene exhibit cleft palate, delayed pulmonary development and neonatal death. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009368.3	NP_033394.2	transforming growth factor beta-3 proprotein precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TGFB3	NP_001094653.1
Mus musculus (house mouse)	Tgfb3	NP_033394.2
Gallus gallus (chicken)	TGFB3	NP_990785.1
Homo sapiens (human)	TGFB3	NP_003230.1
Pan troglodytes (chimpanzee)	TGFB3	XP_001161669.1
	TGFB3	XP_001100053.1
Canis lupus familiaris (dog)	TGFB3	XP_854119.2
Danio rerio (zebrafish)	tgfb3	NP_919367.2
Rattus norvegicus (Norway rat)	Tgfb3	NP_037306.1

Gene Ontology
Bibliography

Related articles in PubMed

Complementary DNA cloning of the murine transforming growth factor-beta 3 (TGF beta 3) precursor and the comparative expression of TGF beta 3 and TGF beta 1 messenger RNA in murine embryos and adult tissues.
Miller DA, Lee A, Matsui Y, Chen EY, Moses HL, Derynck R
Molecular endocrinology (Baltimore, Md.)3(12)1926-34(1989 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data (including data from studies using knockout mice) suggest Garp/Lrrc32 is involved in up-regulation of Tgfb3 and is essential for normal embryogenesis of palate; knockout of Garp causes postnatal lethality, cleft palate, and decreased apoptosis and Smad2 phosphorylation in medial edge epithelial cells of palatal shelf of embryos. (Garp = glycoprotein A repetitions predominant protein; Smad2 = MAD homolog protein 2)
Title: Glycoprotein A repetitions predominant (GARP) positively regulates transforming growth factor (TGF) ?3 and is essential for mouse palatogenesis.

TGFb3-induced down-regulation of p63 in the medial edge epithelia of the palatal shelves is a pre-requisite for palatal fusion by facilitating periderm migration from, and reducing the proliferative potential of, the midline epithelial seam thereby preventing cleft palate.
Title: p63 exerts spatio-temporal control of palatal epithelial cell fate to prevent cleft palate.

Early activation of hepatic stellate cell and imbalance expression of TGF-beta1 and TGF-beta3 existed in ConA-induced acute autoimmune liver injury.
Title: Stellate Cell Activation and Imbalanced Expression of TGF-

We identified TGF-beta3 as the top-ranked gene, a critical component of the transforming growth factor-b (TGF-b) and mitogen activated protein kinase (MAPK) signalling pathways in cardiac fibrosis
Title: Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice.

CCN4 has a positive influence on chondrogenic differentiation by modulating the effects of TGF-beta3.
Title: CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-?3 function.

The following Tgfb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tgfb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16513	NM_009368.3 Latest version!	Mus musculus transforming growth factor, beta 3 (Tgfb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tgfb3 ( NM_009368.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033394.2 Mus musculus transforming growth factor, beta 3 (Tgfb3), mRNA.

Price & Availability↑

CloneID	OMu16513
Clone ID Related Accession (Same CDS sequence)	NM_009368.3
Accession Version	NM_009368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	transforming growth factor beta-3 proprotein precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC134328.2, BC089347.1, AK162560.1 and AW122646.1. On Mar 25, 2009 this sequence version replaced NM_009368.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108426.1, AK145304.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGC ACTTGCAAAG GGCTCTGGTA GTCCTGGCCC TGCTGAACTT GGCCACAATC 
AGCCTCTCTC TGTCCACTTG CACCACGTTG GACTTCGGCC ACATCAAGAA GAAGAGGGTG 
GAAGCCATTA GGGGACAGAT CTTGAGCAAG CTCAGGCTCA CCAGCCCCCC TGAGCCATCG 
GTGATGACCC ACGTCCCCTA TCAGGTCCTG GCACTTTACA ACAGCACCCG GGAGTTGCTG 
GAAGAGATGC ACGGGGAGAG GGAGGAAGGC TGCACTCAGG AGACCTCGGA GTCTGAGTAC 
TATGCCAAAG AGATCCATAA ATTCGACATG ATCCAGGGAC TGGCGGAGCA CAATGAACTG 
GCCGTCTGCC CCAAAGGAAT TACCTCTAAG GTTTTTCGTT TCAATGTGTC CTCAGTGGAG 
AAAAATGGAA CCAATCTGTT CCGGGCAGAG TTCCGGGTCT TGCGGGTGCC CAACCCCAGC 
TCCAAGCGCA CAGAGCAGAG AATTGAGCTC TTCCAGATAC TTCGACCGGA TGAGCACATA 
GCCAAGCAGC GCTACATAGG TGGCAAGAAT CTGCCCACAA GGGGCACCGC TGAATGGCTG 
TCTTTCGATG TCACTGACAC TGTGCGCGAG TGGCTGTTGA GGAGAGAGTC CAACTTGGGT 
CTGGAAATCA GCATCCACTG TCCATGTCAC ACCTTTCAGC CCAATGGAGA CATACTGGAA 
AATGTTCATG AGGTGATGGA AATCAAATTC AAAGGAGTGG ACAATGAAGA TGACCATGGC 
CGTGGAGACC TGGGGCGTCT CAAGAAGCAA AAGGATCACC ACAACCCACA CCTGATCCTC 
ATGATGATCC CCCCACACCG ACTGGACAGC CCAGGCCAGG GCAGTCAGAG GAAGAAGAGG 
GCCCTGGACA CCAATTACTG CTTCCGCAAC CTGGAGGAGA ACTGCTGTGT ACGCCCCCTT 
TATATTGACT TCCGGCAGGA TCTAGGCTGG AAATGGGTCC ACGAACCTAA GGGTTACTAT 
GCCAACTTCT GCTCAGGCCC TTGCCCATAC CTCCGCAGCG CAGACACAAC CCATAGCACG 
GTGCTTGGAC TATACAACAC CCTGAACCCA GAGGCGTCTG CCTCGCCATG CTGCGTCCCC 
CAGGACCTGG AGCCCCTGAC CATCTTGTAC TATGTGGGCA GAACCCCCAA GGTGGAGCAG 
CTGTCCAACA TGGTGGTGAA GTCGTGTAAG TGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033394.2
CDS	1110..2348
Translation	MKMHLQRALVVLALLNLATISLSLSTCTTLDFGHIKKKRVEAIRGQILSKLRLTSPPEPSVMTHVPYQVLALYNSTRELLEEMHGEREEGCTQETSESEYYAKEIHKFDMIQGLAEHNELAVCPKGITSKVFRFNVSSVEKNGTNLFRAEFRVLRVPNPSSKRTEQRIELFQILRPDEHIAKQRYIGGKNLPTRGTAEWLSFDVTDTVREWLLRRESNLGLEISIHCPCHTFQPNGDILENVHEVMEIKFKGVDNEDDHGRGDLGRLKKQKDHHNPHLILMMIPPHRLDSPGQGSQRKKRALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS

Target ORF information:

RefSeq Version	NM_009368.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transforming growth factor, beta 3 (Tgfb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009368.3

ORF Insert Sequence:

ATGAAGATGC ACTTGCAAAG GGCTCTGGTA GTCCTGGCCC TGCTGAACTT GGCCACAATC 
AGCCTCTCTC TGTCCACTTG CACCACGTTG GACTTCGGCC ACATCAAGAA GAAGAGGGTG 
GAAGCCATTA GGGGACAGAT CTTGAGCAAG CTCAGGCTCA CCAGCCCCCC TGAGCCATCG 
GTGATGACCC ACGTCCCCTA TCAGGTCCTG GCACTTTACA ACAGCACCCG GGAGTTGCTG 
GAAGAGATGC ACGGGGAGAG GGAGGAAGGC TGCACTCAGG AGACCTCGGA GTCTGAGTAC 
TATGCCAAAG AGATCCATAA ATTCGACATG ATCCAGGGAC TGGCGGAGCA CAATGAACTG 
GCCGTCTGCC CCAAAGGAAT TACCTCTAAG GTTTTTCGTT TCAATGTGTC CTCAGTGGAG 
AAAAATGGAA CCAATCTGTT CCGGGCAGAG TTCCGGGTCT TGCGGGTGCC CAACCCCAGC 
TCCAAGCGCA CAGAGCAGAG AATTGAGCTC TTCCAGATAC TTCGACCGGA TGAGCACATA 
GCCAAGCAGC GCTACATAGG TGGCAAGAAT CTGCCCACAA GGGGCACCGC TGAATGGCTG 
TCTTTCGATG TCACTGACAC TGTGCGCGAG TGGCTGTTGA GGAGAGAGTC CAACTTGGGT 
CTGGAAATCA GCATCCACTG TCCATGTCAC ACCTTTCAGC CCAATGGAGA CATACTGGAA 
AATGTTCATG AGGTGATGGA AATCAAATTC AAAGGAGTGG ACAATGAAGA TGACCATGGC 
CGTGGAGACC TGGGGCGTCT CAAGAAGCAA AAGGATCACC ACAACCCACA CCTGATCCTC 
ATGATGATCC CCCCACACCG ACTGGACAGC CCAGGCCAGG GCAGTCAGAG GAAGAAGAGG 
GCCCTGGACA CCAATTACTG CTTCCGCAAC CTGGAGGAGA ACTGCTGTGT ACGCCCCCTT 
TATATTGACT TCCGGCAGGA TCTAGGCTGG AAATGGGTCC ACGAACCTAA GGGTTACTAT 
GCCAACTTCT GCTCAGGCCC TTGCCCATAC CTCCGCAGCG CAGACACAAC CCATAGCACG 
GTGCTTGGAC TATACAACAC CCTGAACCCA GAGGCGTCTG CCTCGCCATG CTGCGTCCCC 
CAGGACCTGG AGCCCCTGAC CATCTTGTAC TATGTGGGCA GAACCCCCAA GGTGGAGCAG 
CTGTCCAACA TGGTGGTGAA GTCGTGTAAG TGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Complementary DNA cloning of the murine transforming growth factor-beta 3 (TGF beta 3) precursor and the comparative expression of TGF beta 3 and TGF beta 1 messenger RNA in murine embryos and adult tissues.
Molecular endocrinology (Baltimore, Md.)3(12)1926-34(1989 Dec)
Miller DA,Lee A,Matsui Y,Chen EY,Moses HL,Derynck R

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Tgfb3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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