Cdc34 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc34
Entrez Gene ID	216150
Full Name	cell division cycle 34
Synonyms	AI327276,E2-CDC34,UBE2R1
General protein information	Preferred Names cell division cycle 34 Names ubiquitin-conjugating enzyme E2 R1 (E3-independent) E2 ubiquitin-conjugating enzyme R1 E2 ubiquitin-conjugating enzyme R1 cell division cycle 34 homolog ubiquitin-conjugating enzyme Cdc34 ubiquitin-conjugating enzyme E2-32 kDa complementing ubiquitin-conjugating enzyme E2-CDC34 ubiquitin-protein ligase R1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C1\|10 39.72 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017313887.1	XP_017169376.1	ubiquitin-conjugating enzyme E2 R1 isoform X1
NM_001359817.1	NP_001346746.1	ubiquitin-conjugating enzyme E2 R1 isoform 2
XM_017313888.2	XP_017169377.1	ubiquitin-conjugating enzyme E2 R1 isoform X1
XM_006513483.2	XP_006513546.1	ubiquitin-conjugating enzyme E2 R1 isoform X3
XM_017313888.1	XP_017169377.1	ubiquitin-conjugating enzyme E2 R1 isoform X1
NM_001359818.1	NP_001346747.1	ubiquitin-conjugating enzyme E2 R1 isoform 3
NM_177613.2	NP_808281.1	ubiquitin-conjugating enzyme E2 R1 isoform 1
XM_006513482.2	XP_006513545.1	ubiquitin-conjugating enzyme E2 R1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cdc34	NP_808281.1
Gallus gallus (chicken)	CDC34	XP_423237.2
	LOC100425016	XP_002801037.1
Danio rerio (zebrafish)	cdc34a	NP_957252.1
Danio rerio (zebrafish)	cdc34b	NP_001002688.1
Homo sapiens (human)	CDC34	NP_004350.1
Bos taurus (cattle)	CDC34	NP_001193165.1
Rattus norvegicus (Norway rat)	Cdc34	NP_001013121.2
Xenopus tropicalis (tropical clawed frog)	cdc34	NP_001004793.1
Canis lupus familiaris (dog)	CDC34	XP_533965.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Endogenous muscle atrophy F-box mediates pressure overload-induced cardiac hypertrophy through regulation of nuclear factor-kappaB.
Usui S, Maejima Y, Pain J, Hong C, Cho J, Park JY, Zablocki D, Tian B, Glass DJ, Sadoshima J
Circulation research109(2)161-71(2011 Jul)

A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.
Flores-Delgado G, Liu CW, Sposto R, Berndt N
Journal of proteome research6(3)1165-75(2007 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cdc34 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc34 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu44382	NM_001359817.1 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu44383	NM_001359818.1 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu44382	XM_006513482.2 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu44383	XM_006513483.2 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu06554	NM_177613.2 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu06554	XM_017313888.2 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu06554	XM_017313887.1 Latest version!	Mus musculus cell division cycle 34 (Cdc34), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu06554	XM_017313888.1	Mus musculus cell division cycle 34 (Cdc34), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc34 ( NM_001359817.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346746.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu44382
Clone ID Related Accession (Same CDS sequence)	NM_001359817.1 , XM_006513482.2
Accession Version	NM_001359817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151828.3, BY114161.1, BY083281.1, CA320111.1 and BE988402.1. On Jan 18, 2018 this sequence version replaced XM_006513482.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.432683.1, CF550927.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGGAAGCAGG TCCTGGGGAC CAAGGTGGAC GCGGAGCGCG ATGGTGTGAA GGTGCCCACT 
ACGCTGGCCG AGTACTGCGT GAAGACCAAG GCGCCGGCGC CCGACGAGGG CTCGGACCTC 
TTCTACGACG ACTACTATGA GGACGGCGAA GTGGAGGAGG CCGACAGCTG CTTTGGGGAT 
GAAGAGGATG ACTCTGGCAC CGAAGAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346746.1
CDS	220..792
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES

Target ORF information:

RefSeq Version	NM_001359817.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359817.1

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGGAAGCAGG TCCTGGGGAC CAAGGTGGAC GCGGAGCGCG ATGGTGTGAA GGTGCCCACT 
ACGCTGGCCG AGTACTGCGT GAAGACCAAG GCGCCGGCGC CCGACGAGGG CTCGGACCTC 
TTCTACGACG ACTACTATGA GGACGGCGAA GTGGAGGAGG CCGACAGCTG CTTTGGGGAT 
GAAGAGGATG ACTCTGGCAC CGAAGAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( NM_001359818.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346747.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu44383
Clone ID Related Accession (Same CDS sequence)	XM_006513483.2 , NM_001359818.1
Accession Version	NM_001359818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151828.3, BY114161.1, CX210748.1, CA320111.1 and BE988402.1. On Jan 18, 2018 this sequence version replaced XM_006513483.2. ##Evidence-Data-START## Transcript exon combination :: BF303478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGCAG GTCCTGGGGA CCAAGGTGGA CGCGGAGCGC GATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346747.1
CDS	220..759
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRRSWGPRWTRSAMV

Target ORF information:

RefSeq Version	NM_001359818.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359818.1

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGCAG GTCCTGGGGA CCAAGGTGGA CGCGGAGCGC GATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( XM_006513482.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513545.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu44382
Clone ID Related Accession (Same CDS sequence)	NM_001359817.1 , XM_006513482.2
Accession Version	XM_006513482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006513482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGGAAGCAGG TCCTGGGGAC CAAGGTGGAC GCGGAGCGCG ATGGTGTGAA GGTGCCCACT 
ACGCTGGCCG AGTACTGCGT GAAGACCAAG GCGCCGGCGC CCGACGAGGG CTCGGACCTC 
TTCTACGACG ACTACTATGA GGACGGCGAA GTGGAGGAGG CCGACAGCTG CTTTGGGGAT 
GAAGAGGATG ACTCTGGCAC CGAAGAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513545.1
CDS	212..784
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES

Target ORF information:

RefSeq Version	XM_006513482.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513482.2

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGGAAGCAGG TCCTGGGGAC CAAGGTGGAC GCGGAGCGCG ATGGTGTGAA GGTGCCCACT 
ACGCTGGCCG AGTACTGCGT GAAGACCAAG GCGCCGGCGC CCGACGAGGG CTCGGACCTC 
TTCTACGACG ACTACTATGA GGACGGCGAA GTGGAGGAGG CCGACAGCTG CTTTGGGGAT 
GAAGAGGATG ACTCTGGCAC CGAAGAGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( XM_006513483.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513546.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu44383
Clone ID Related Accession (Same CDS sequence)	XM_006513483.2 , NM_001359818.1
Accession Version	XM_006513483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006513483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGCAG GTCCTGGGGA CCAAGGTGGA CGCGGAGCGC GATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513546.1
CDS	212..751
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRRSWGPRWTRSAMV

Target ORF information:

RefSeq Version	XM_006513483.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513483.2

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGCAG GTCCTGGGGA CCAAGGTGGA CGCGGAGCGC GATGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( NM_177613.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808281.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06554
Clone ID Related Accession (Same CDS sequence)	XM_017313887.1 , XM_017313888.2 , XM_017313888.1 , NM_177613.2
Accession Version	NM_177613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC039160.1. On or before Dec 7, 2005 this sequence version replaced XM_922791.1, XM_922795.1, XM_922801.1, XM_922808.1, XM_922814.1, XM_922817.1, XM_913571.1, NM_177613.1. ##Evidence-Data-START## Transcript exon combination :: BC039160.1, CA984649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808281.1
CDS	220..927
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES

Target ORF information:

RefSeq Version	NM_177613.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177613.2

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( XM_017313887.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169376.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant X1, mRNA.

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CloneID	OMu06554
Clone ID Related Accession (Same CDS sequence)	XM_017313887.1 , XM_017313888.2 , XM_017313888.1 , NM_177613.2
Accession Version	XM_017313887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169376.1
CDS	48..755
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES

Target ORF information:

RefSeq Version	XM_017313887.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313887.1

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( XM_017313888.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169377.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu06554
Clone ID Related Accession (Same CDS sequence)	XM_017313887.1 , XM_017313888.2 , XM_017313888.1 , NM_177613.2
Accession Version	XM_017313888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169377.1
CDS	56..763
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES

Target ORF information:

RefSeq Version	XM_017313888.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313888.2

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc34 ( XM_017313888.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169377.1 Mus musculus cell division cycle 34 (Cdc34), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu06554
Clone ID Related Accession (Same CDS sequence)	XM_017313887.1 , XM_017313888.2 , XM_017313888.1 , NM_177613.2
Accession Version	XM_017313888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 R1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169377.1
CDS	55..762
Translation	MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEADSCFGDEEDDSGTEES

Target ORF information:

RefSeq Version	XM_017313888.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 34 (Cdc34), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313888.1

ORF Insert Sequence:

ATGGCCCGGC CGCTGGTGCC AAGCTCGCAG AAGGCGCTGC TGCTGGAACT GAAGGGGCTG 
CAGGAGGAGC CGGTGGAGGG CTTCCGGGTG ACGCTGGTGG ACGAGGGTGA CCTGTACAAC 
TGGGAGGTGG CCATCTTCGG GCCCCCCAAC ACCTACTATG AGGGCGGCTA CTTCAAGGCT 
CGCCTCAAGT TCCCCATCGA CTACCCGTAT TCCCCACCAG CCTTCCGGTT CCTCACCAAG 
ATGTGGCACC CAAACATCTA TGAGACAGGG GACGTGTGCA TCTCCATTCT CCATCCCCCA 
GTTGACGACC CACAGAGTGG GGAGCTGCCC TCAGAGCGGT GGAACCCCAC ACAGAATGTC 
AGAACCATCC TCCTGAGTGT AATTTCGCTG CTGAATGAGC CCAACACCTT CTCGCCTGCC 
AACGTGGACG CCTCGGTGAT GTACAGAAAA TGGAAGGAGA GCAAGGGGAA GGACCGCGAG 
TACACGGACA TCATCCGGAA GCAGGTCCTG GGGACCAAGG TGGACGCGGA GCGCGATGGT 
GTGAAGGTGC CCACTACGCT GGCCGAGTAC TGCGTGAAGA CCAAGGCGCC GGCGCCCGAC 
GAGGGCTCGG ACCTCTTCTA CGACGACTAC TATGAGGACG GCGAAGTGGA GGAGGCCGAC 
AGCTGCTTTG GGGATGAAGA GGATGACTCT GGCACCGAAG AGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Endogenous muscle atrophy F-box mediates pressure overload-induced cardiac hypertrophy through regulation of nuclear factor-kappaB.
Circulation research109(2)161-71(2011 Jul)
Usui S,Maejima Y,Pain J,Hong C,Cho J,Park JY,Zablocki D,Tian B,Glass DJ,Sadoshima J

A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.
Journal of proteome research6(3)1165-75(2007 Mar)
Flores-Delgado G,Liu CW,Sposto R,Berndt N

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Cdc34 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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