Vezt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vezt
Entrez Gene ID	215008
Full Name	vezatin, adherens junctions transmembrane protein
Synonyms	A630071D13Rik,AI848282
General protein information	Preferred Names vezatin, adherens junctions transmembrane protein Names vezatin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C2
Summary	This gene encodes a transmembrane protein that is essential for the formation of adherens junctions. It is required for both the pre-implantation morphogenesis of a blastocyst and for the implantation process. The encoded protein is also a component of the ankle-link complex in cochlear hair cells, where it may effect resilience to sound trauma. It is also thought to be involved in dendritic spine morphogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001304572.1	NP_001291501.1	vezatin isoform 2
NM_172538.5	NP_766126.2	vezatin isoform 1
NM_001304720.1	NP_001291649.1	vezatin isoform 4
NM_001304575.1	NP_001291504.1	vezatin isoform 3

Pathways from BioSystems

Homologies

	VEZT	XP_001107542.2
Rattus norvegicus (Norway rat)	Vezt	NP_001006985.1
Xenopus tropicalis (tropical clawed frog)	vezt	NP_001039208.1
Homo sapiens (human)	VEZT	NP_060069.3
Pan troglodytes (chimpanzee)	VEZT	XP_001144315.1
Canis lupus familiaris (dog)	VEZT	XP_005629290.1
Mus musculus (house mouse)	Vezt	NP_766126.2
Danio rerio (zebrafish)	vetz	NP_001108047.1
Bos taurus (cattle)	VEZT	XP_002687252.1
Gallus gallus (chicken)	VEZT	XP_416152.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation.
Danglot L, Freret T, Le Roux N, Narboux N?me N, Burgo A, Hyenne V, Roumier A, Contremoulins V, Dauphin F, Bizot JC, Vodjdani G, Gaspar P, Boulouard M, Poncer JC, Galli T, Simmler MC
The Journal of neuroscience : the official journal of the Society for Neuroscience32(26)9007-22(2012 Jun)

Vezatin, a potential target for ADP-ribosylation factor 6, regulates the dendritic formation of hippocampal neurons.
Sanda M, Ohara N, Kamata A, Hara Y, Tamaki H, Sukegawa J, Yanagisawa T, Fukunaga K, Kondo H, Sakagami H
Neuroscience research67(2)126-36(2010 Jun)

Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.
Bahloul A, Simmler MC, Michel V, Leibovici M, Perfettini I, Roux I, Weil D, Nouaille S, Zuo J, Zadro C, Licastro D, Gasparini P, Avan P, Hardelin JP, Petit C
EMBO molecular medicine1(2)125-38(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest a role of Vezatin in dendritic spine morphogenesis and functional synaptic maturation.
Title: Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation.

present study suggests that vezatin may regulate dendritic formation as a downstream effector of ARF6.
Title: Vezatin, a potential target for ADP-ribosylation factor 6, regulates the dendritic formation of hippocampal neurons.

vezatin associates with actin filaments at cell-cell junctions.
Title: Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.

These in vitro observations reinforce the idea that homozygous vezatin-null mutants die at the time of implantation because of a defect in intercellular adhesion.
Title: Conditional knock-out reveals that zygotic vezatin-null mouse embryos die at implantation.

Required for morphogenesis of the preimplantation mouse embryo.
Title: Vezatin, a protein associated to adherens junctions, is required for mouse blastocyst morphogenesis.

The following Vezt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vezt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu72916	NM_001304572.1 Latest version!	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu72918	NM_001304720.1 Latest version!	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu72917	NM_001304575.1 Latest version!	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu03637	NM_172538.5 Latest version!	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$461.30 $659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vezt ( NM_001304572.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291501.1 Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu72916
Clone ID Related Accession (Same CDS sequence)	NM_001304572.1
Accession Version	NM_001304572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	vezatin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122901.4, BC056428.1, BQ555053.1, AK031192.1, AK042209.1 and AW124866.1. On Feb 15, 2015 this sequence version replaced XM_006513465.2. Transcript Variant: This variant (2) uses an alternate splice site in the 3' terminal exon compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY753562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT GGTCAGTGCT 
GCTTGCTCAT TTAATAAGGC TGCACAGCAT CCCGGCCAGC ATCTCATCGG CCTTCGGAAA 
GCCGTCTACA GGACCGTAAG AGCCAACTTT CAAGCCGCAA GGCTAGCTAC CTTGTACATG 
CTGAAAAACT ACCCCCTGAA CTCTGAGAGT GACAATGTCA CCAACTACAT CTGTGTGGTA 
CCTTTTAAGG AGCTGGGGCT TGGACTCAGT GAAGACCAGA TCTCAGAAGA GGAAGCTCGT 
AACCTGACAG ATGGCTTCAG CCTGCCTGCC TTGAAGGTTT TGTTCCAGCT CTGGGTGGCA 
CAGAGTTCAG AGTTCTTCAG ACGCCTGGCC CTGTTACTTT CTACAGCGAA TTCGCCTTCA 
GGGCCCTTAC TCACTGCTGC ACTTCTGCCT CATCATATCC TCTGTGACGT GACTCAAGGT 
CTACCTCATG CTCACTCTGC CTGCTTGGAT GAGCTGAAGC GCAGCTATGA GTTCTTCCGG 
TACTTTGAAA CTCAGCACCA GTCAGTCCCC CAGCGCTTAT CCAAGACTCC GCAGAAGTCG 
AGGGAGCTGA GCAACGTCCA CACAGCAGTC CGTAGCTTAC AGCTCCATCT GAAAGCACTA 
CTGAATGAAG TAATAATTCT TGAAGATGAA CTTGAAAAGC TCGTTTGTAC TAAAGAAACA 
CAAGAGCTGC TATCGGAGGC TTATCCGATA CTAGAGCAGA AATTAAAACT GATTGAACCA 
CACGTTCAGG CCAGCAACAG TTGCTGGGAG GAAGCCATTT CTCAAGTGGA TAAACTGCTC 
CGGAGGAACA CAGATAAAAA AGGCAAGCCT GGAGTAGCCT GTGAGAACCC ACACTGCACC 
GCGGAGCCCT TGGTGCGGCC AGCTCTACAC ATTGAGGACA GAGACCCCAT CCCTGAGGAG 
CAGGAATTAG AAGCTTATGT GGATGACATA GACATAGAAA GTGAGTTCCG AAAGGACGAT 
TTCTATCACT TGTCTCAGGA AGACAGAGAG AGACAGAAGC GCGAGCAGGA GGAGTCCAGG 
CGGGTGCTGC AGGAGCTGAA GTCTGTGCTG GGATTTAAGG CTTCCGAGGC TGAGAGGCAG 
AAGTGGAAGC AGCTGCTGTT CAGTGACCAC GGGGTAAAGG CCGAGTGGAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291501.1
CDS	95..1948
Translation	MTPEFDEEVVFENSPLYQYLQDLGHTDFEICSSSSPKPEKCLTTEGPQPPPTRVLQRQGILLKLTETIKSWTFSSQHSKKDDLLHKLDTGFRLDSLHTILQQEVLLQEDVELLELLDASILSAGQPQQESGHLPTLCSLATPNTWDVSLLFAFISLLIMFPTCWIVSSWLVWGIILFLYLIIRVLKLWRTAKLQMTLKKYRVRLEDMAANSRAFTNLVRKSLRLIQETEVISRGFTLVSAACSFNKAAQHPGQHLIGLRKAVYRTVRANFQAARLATLYMLKNYPLNSESDNVTNYICVVPFKELGLGLSEDQISEEEARNLTDGFSLPALKVLFQLWVAQSSEFFRRLALLLSTANSPSGPLLTAALLPHHILCDVTQGLPHAHSACLDELKRSYEFFRYFETQHQSVPQRLSKTPQKSRELSNVHTAVRSLQLHLKALLNEVIILEDELEKLVCTKETQELLSEAYPILEQKLKLIEPHVQASNSCWEEAISQVDKLLRRNTDKKGKPGVACENPHCTAEPLVRPALHIEDRDPIPEEQELEAYVDDIDIESEFRKDDFYHLSQEDRERQKREQEESRRVLQELKSVLGFKASEAERQKWKQLLFSDHGVKAEWN

Target ORF information:

RefSeq Version	NM_001304572.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304572.1

ORF Insert Sequence:

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT GGTCAGTGCT 
GCTTGCTCAT TTAATAAGGC TGCACAGCAT CCCGGCCAGC ATCTCATCGG CCTTCGGAAA 
GCCGTCTACA GGACCGTAAG AGCCAACTTT CAAGCCGCAA GGCTAGCTAC CTTGTACATG 
CTGAAAAACT ACCCCCTGAA CTCTGAGAGT GACAATGTCA CCAACTACAT CTGTGTGGTA 
CCTTTTAAGG AGCTGGGGCT TGGACTCAGT GAAGACCAGA TCTCAGAAGA GGAAGCTCGT 
AACCTGACAG ATGGCTTCAG CCTGCCTGCC TTGAAGGTTT TGTTCCAGCT CTGGGTGGCA 
CAGAGTTCAG AGTTCTTCAG ACGCCTGGCC CTGTTACTTT CTACAGCGAA TTCGCCTTCA 
GGGCCCTTAC TCACTGCTGC ACTTCTGCCT CATCATATCC TCTGTGACGT GACTCAAGGT 
CTACCTCATG CTCACTCTGC CTGCTTGGAT GAGCTGAAGC GCAGCTATGA GTTCTTCCGG 
TACTTTGAAA CTCAGCACCA GTCAGTCCCC CAGCGCTTAT CCAAGACTCC GCAGAAGTCG 
AGGGAGCTGA GCAACGTCCA CACAGCAGTC CGTAGCTTAC AGCTCCATCT GAAAGCACTA 
CTGAATGAAG TAATAATTCT TGAAGATGAA CTTGAAAAGC TCGTTTGTAC TAAAGAAACA 
CAAGAGCTGC TATCGGAGGC TTATCCGATA CTAGAGCAGA AATTAAAACT GATTGAACCA 
CACGTTCAGG CCAGCAACAG TTGCTGGGAG GAAGCCATTT CTCAAGTGGA TAAACTGCTC 
CGGAGGAACA CAGATAAAAA AGGCAAGCCT GGAGTAGCCT GTGAGAACCC ACACTGCACC 
GCGGAGCCCT TGGTGCGGCC AGCTCTACAC ATTGAGGACA GAGACCCCAT CCCTGAGGAG 
CAGGAATTAG AAGCTTATGT GGATGACATA GACATAGAAA GTGAGTTCCG AAAGGACGAT 
TTCTATCACT TGTCTCAGGA AGACAGAGAG AGACAGAAGC GCGAGCAGGA GGAGTCCAGG 
CGGGTGCTGC AGGAGCTGAA GTCTGTGCTG GGATTTAAGG CTTCCGAGGC TGAGAGGCAG 
AAGTGGAAGC AGCTGCTGTT CAGTGACCAC GGGGTAAAGG CCGAGTGGAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vezt ( NM_001304720.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291649.1 Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu72918
Clone ID Related Accession (Same CDS sequence)	NM_001304720.1
Accession Version	NM_001304720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	vezatin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK042540.1, CF730355.1, BQ555053.1, AC122901.4, AK031192.1, AK042209.1 and AW124866.1. Transcript Variant: This variant (4) uses an alternate splice site in the 3' terminal exon compared to variant 1. It encodes isoform 4, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK042540.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT GGTCAGTGCT 
GCTTGCTCAT TTAATAAGGC TGCACAGCAT CCCGGCCAGC ATCTCATCGG CCTTCGGAAA 
GCCGTCTACA GGACCGTAAG AGCCAACTTT CAAGCCGCAA GGCTAGCTAC CTTGTACATG 
CTGAAAAACT ACCCCCTGAA CTCTGAGAGT GACAATGTCA CCAACTACAT CTGTGTGGTA 
CCTTTTAAGG AGCTGGGGCT TGGACTCAGT GAAGACCAGA TCTCAGAAGA GGAAGCTCGT 
AACCTGACAG ATGGCTTCAG CCTGCCTGCC TTGAAGGTTT TGTTCCAGCT CTGGGTGGCA 
CAGAGTTCAG AGTTCTTCAG ACGCCTGGCC CTGTTACTTT CTACAGCGAA TTCGCCTTCA 
GGGCCCTTAC TCACTGCTGC ACTTCTGCCT CATCATATCC TCTGTGACGT GACTCAAGGT 
CTACCTCATG CTCACTCTGC CTGCTTGGAT GAGCTGAAGC GCAGCTATGA GTTCTTCCGG 
TACTTTGAAA CTCAGCACCA GTCAGTCCCC CAGCGCTTAT CCAAGACTCC GCAGAAGTCG 
AGGGAGCTGA GCAACGTCCA CACAGCAGTC CGTAGCTTAC AGCTCCATCT GAAAGCACTA 
CTGAATGAAG TAATAATTCT TGAAGATGAA CTTGAAAAGC TCGTTTGTAC TAAAGAAACA 
CAAGAGCTGC TATCGGAGGC TTATCCGATA CTAGAGCAGA AATTAAAACT GATTGAACCA 
CACGTTCAGG CCAGCAACAG TTGCTGGGAG GAAGCCATTT CTCAAGTGGA TAAACTGCTC 
CGGAGGAACA CAGATAAAAA AGGCAAGCCT GGAGTAGCCT GTGAGAACCC ACACTGCACC 
GCGGAGCCCT TGGTGCGGCC AGCTCTACAC ATTGAGGACA GAGACCCCAT CCCTGAGGAG 
CAGGAATTAG AAGCTTATGT GGATGACATA GACATAGAAA GTGAGTTCCG AAAGGACGAT 
TTCTATCACT TGTCTCAGGA AGACAGAGAG AGACAGAAGC GCGAGCAGGA GGAGTCCAGG 
CGGGTGCTGC AGGAGCTGAA GTCTGTGCTG GGATTTAAGG CTTCCGAGGC TGAGAGGCAG 
AAGTGGAAGC AGCTGCTGTT CAGTGACCAC GAGTGGACCT TTAATTGGAT ACATCATGCT 
GGCCATGGTG GCACTGTAAT CCCAGCAAGG TGGTTGAGGC AAGGAGGATC AGGAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291649.1
CDS	38..1957
Translation	MTPEFDEEVVFENSPLYQYLQDLGHTDFEICSSSSPKPEKCLTTEGPQPPPTRVLQRQGILLKLTETIKSWTFSSQHSKKDDLLHKLDTGFRLDSLHTILQQEVLLQEDVELLELLDASILSAGQPQQESGHLPTLCSLATPNTWDVSLLFAFISLLIMFPTCWIVSSWLVWGIILFLYLIIRVLKLWRTAKLQMTLKKYRVRLEDMAANSRAFTNLVRKSLRLIQETEVISRGFTLVSAACSFNKAAQHPGQHLIGLRKAVYRTVRANFQAARLATLYMLKNYPLNSESDNVTNYICVVPFKELGLGLSEDQISEEEARNLTDGFSLPALKVLFQLWVAQSSEFFRRLALLLSTANSPSGPLLTAALLPHHILCDVTQGLPHAHSACLDELKRSYEFFRYFETQHQSVPQRLSKTPQKSRELSNVHTAVRSLQLHLKALLNEVIILEDELEKLVCTKETQELLSEAYPILEQKLKLIEPHVQASNSCWEEAISQVDKLLRRNTDKKGKPGVACENPHCTAEPLVRPALHIEDRDPIPEEQELEAYVDDIDIESEFRKDDFYHLSQEDRERQKREQEESRRVLQELKSVLGFKASEAERQKWKQLLFSDHEWTFNWIHHAGHGGTVIPARWLRQGGSGV

Target ORF information:

RefSeq Version	NM_001304720.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304720.1

ORF Insert Sequence:

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT GGTCAGTGCT 
GCTTGCTCAT TTAATAAGGC TGCACAGCAT CCCGGCCAGC ATCTCATCGG CCTTCGGAAA 
GCCGTCTACA GGACCGTAAG AGCCAACTTT CAAGCCGCAA GGCTAGCTAC CTTGTACATG 
CTGAAAAACT ACCCCCTGAA CTCTGAGAGT GACAATGTCA CCAACTACAT CTGTGTGGTA 
CCTTTTAAGG AGCTGGGGCT TGGACTCAGT GAAGACCAGA TCTCAGAAGA GGAAGCTCGT 
AACCTGACAG ATGGCTTCAG CCTGCCTGCC TTGAAGGTTT TGTTCCAGCT CTGGGTGGCA 
CAGAGTTCAG AGTTCTTCAG ACGCCTGGCC CTGTTACTTT CTACAGCGAA TTCGCCTTCA 
GGGCCCTTAC TCACTGCTGC ACTTCTGCCT CATCATATCC TCTGTGACGT GACTCAAGGT 
CTACCTCATG CTCACTCTGC CTGCTTGGAT GAGCTGAAGC GCAGCTATGA GTTCTTCCGG 
TACTTTGAAA CTCAGCACCA GTCAGTCCCC CAGCGCTTAT CCAAGACTCC GCAGAAGTCG 
AGGGAGCTGA GCAACGTCCA CACAGCAGTC CGTAGCTTAC AGCTCCATCT GAAAGCACTA 
CTGAATGAAG TAATAATTCT TGAAGATGAA CTTGAAAAGC TCGTTTGTAC TAAAGAAACA 
CAAGAGCTGC TATCGGAGGC TTATCCGATA CTAGAGCAGA AATTAAAACT GATTGAACCA 
CACGTTCAGG CCAGCAACAG TTGCTGGGAG GAAGCCATTT CTCAAGTGGA TAAACTGCTC 
CGGAGGAACA CAGATAAAAA AGGCAAGCCT GGAGTAGCCT GTGAGAACCC ACACTGCACC 
GCGGAGCCCT TGGTGCGGCC AGCTCTACAC ATTGAGGACA GAGACCCCAT CCCTGAGGAG 
CAGGAATTAG AAGCTTATGT GGATGACATA GACATAGAAA GTGAGTTCCG AAAGGACGAT 
TTCTATCACT TGTCTCAGGA AGACAGAGAG AGACAGAAGC GCGAGCAGGA GGAGTCCAGG 
CGGGTGCTGC AGGAGCTGAA GTCTGTGCTG GGATTTAAGG CTTCCGAGGC TGAGAGGCAG 
AAGTGGAAGC AGCTGCTGTT CAGTGACCAC GAGTGGACCT TTAATTGGAT ACATCATGCT 
GGCCATGGTG GCACTGTAAT CCCAGCAAGG TGGTTGAGGC AAGGAGGATC AGGAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vezt ( NM_001304575.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291504.1 Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu72917
Clone ID Related Accession (Same CDS sequence)	NM_001304575.1
Accession Version	NM_001304575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	vezatin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK031848.1, BC056428.1, AK036017.1, AC122901.4, AK031192.1, AK042209.1 and AW124866.1. On Feb 15, 2015 this sequence version replaced XM_006513463.2. Transcript Variant: This variant (3) uses an alternate splice site in the central coding region, compared to variant 1. It encodes isoform 3, which is longer and has a distinct internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031848.1, SRR7652917.89609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT TTTGCTTGAC 
AGGGTCAGTG CTGCTTGCTC ATTTAATAAG GCTGCACAGC ATCCCGGCCA GCATCTCATC 
GGCCTTCGGA AAGCCGTCTA CAGGACCGTA AGAGCCAACT TTCAAGCCGC AAGGCTAGCT 
ACCTTGTACA TGCTGAAAAA CTACCCCCTG AACTCTGAGA GTGACAATGT CACCAACTAC 
ATCTGTGTGG TACCTTTTAA GGAGCTGGGG CTTGGACTCA GTGAAGACCA GATCTCAGAA 
GAGGAAGCTC GTAACCTGAC AGATGGCTTC AGCCTGCCTG CCTTGAAGGT TTTGTTCCAG 
CTCTGGGTGG CACAGAGTTC AGAGTTCTTC AGACGCCTGG CCCTGTTACT TTCTACAGCG 
AATTCGCCTT CAGGGCCCTT ACTCACTGCT GCACTTCTGC CTCATCATAT CCTCTGTGAC 
GTGACTCAAG GTCTACCTCA TGCTCACTCT GCCTGCTTGG ATGAGCTGAA GCGCAGCTAT 
GAGTTCTTCC GGTACTTTGA AACTCAGCAC CAGTCAGTCC CCCAGCGCTT ATCCAAGACT 
CCGCAGAAGT CGAGGGAGCT GAGCAACGTC CACACAGCAG TCCGTAGCTT ACAGCTCCAT 
CTGAAAGCAC TACTGAATGA AGTAATAATT CTTGAAGATG AACTTGAAAA GCTCGTTTGT 
ACTAAAGAAA CACAAGAGCT GCTATCGGAG GCTTATCCGA TACTAGAGCA GAAATTAAAA 
CTGATTGAAC CACACGTTCA GGCCAGCAAC AGTTGCTGGG AGGAAGCCAT TTCTCAAGTG 
GATAAACTGC TCCGGAGGAA CACAGATAAA AAAGGCAAGC CTGGAGTAGC CTGTGAGAAC 
CCACACTGCA CCGCGGAGCC CTTGGTGCGG CCAGCTCTAC ACATTGAGGA CAGAGACCCC 
ATCCCTGAGG AGCAGGAATT AGAAGCTTAT GTGGATGACA TAGACATAGA AAGTGAGTTC 
CGAAAGGACG ATTTCTATCA CTTGTCTCAG GAAGACAGAG AGAGACAGAA GCGCGAGCAG 
GAGGAGTCCA GGCGGGTGCT GCAGGAGCTG AAGTCTGTGC TGGGATTTAA GGCTTCCGAG 
GCTGAGAGGC AGAAGTGGAA GCAGCTGCTG TTCAGTGACC ACGCTGTGTT GACATCCTTG 
TCTCCTGTAG ACCCAGTGGA ATCTGTAAGT AACTCAGAAC CACCCATGAA TTCAGATACA 
GAAAAAGTCA ATAGTAATGC TACTGAAGAG GAGACTAGTA AGCCCTGTGC CGGCGACAAG 
GAAGACAGTA GGACTGAGTA TGTGTGTGAC AGCCCAACAG AGGGGCCAAG CAAAGACACC 
TCTGCAGACA CAGGCCTCCT CCTTCCAGGA GCCGAGGAAA CCATGTGCCA CCAGCATGAG 
AGTGAGGCTA AGTCTCCTCA GGCTGCTGCT GCGGGGGCCA CTGCCCCTCC GACTCCCAGG 
GACACACTGC AACTCTCCAT TAAGCAGAGG CTGGCTCGAC TCCAGTTGCC ACCAGAATTC 
ACCTTCTCTG CTGGCCTGGC TGCAGAAGTG GCCGCCCGGT CCCTGTCCTT TACCACCATG 
CAGGAACAAA CTTTTGGCGA CGAGGAGGAA GAACAGCTAG TTGAAGGCGG TGAAAATGAG 
GTGGAGGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291504.1
CDS	95..2449
Translation	MTPEFDEEVVFENSPLYQYLQDLGHTDFEICSSSSPKPEKCLTTEGPQPPPTRVLQRQGILLKLTETIKSWTFSSQHSKKDDLLHKLDTGFRLDSLHTILQQEVLLQEDVELLELLDASILSAGQPQQESGHLPTLCSLATPNTWDVSLLFAFISLLIMFPTCWIVSSWLVWGIILFLYLIIRVLKLWRTAKLQMTLKKYRVRLEDMAANSRAFTNLVRKSLRLIQETEVISRGFTLLLDRVSAACSFNKAAQHPGQHLIGLRKAVYRTVRANFQAARLATLYMLKNYPLNSESDNVTNYICVVPFKELGLGLSEDQISEEEARNLTDGFSLPALKVLFQLWVAQSSEFFRRLALLLSTANSPSGPLLTAALLPHHILCDVTQGLPHAHSACLDELKRSYEFFRYFETQHQSVPQRLSKTPQKSRELSNVHTAVRSLQLHLKALLNEVIILEDELEKLVCTKETQELLSEAYPILEQKLKLIEPHVQASNSCWEEAISQVDKLLRRNTDKKGKPGVACENPHCTAEPLVRPALHIEDRDPIPEEQELEAYVDDIDIESEFRKDDFYHLSQEDRERQKREQEESRRVLQELKSVLGFKASEAERQKWKQLLFSDHAVLTSLSPVDPVESVSNSEPPMNSDTEKVNSNATEEETSKPCAGDKEDSRTEYVCDSPTEGPSKDTSADTGLLLPGAEETMCHQHESEAKSPQAAAAGATAPPTPRDTLQLSIKQRLARLQLPPEFTFSAGLAAEVAARSLSFTTMQEQTFGDEEEEQLVEGGENEVEEK

Target ORF information:

RefSeq Version	NM_001304575.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304575.1

ORF Insert Sequence:

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT TTTGCTTGAC 
AGGGTCAGTG CTGCTTGCTC ATTTAATAAG GCTGCACAGC ATCCCGGCCA GCATCTCATC 
GGCCTTCGGA AAGCCGTCTA CAGGACCGTA AGAGCCAACT TTCAAGCCGC AAGGCTAGCT 
ACCTTGTACA TGCTGAAAAA CTACCCCCTG AACTCTGAGA GTGACAATGT CACCAACTAC 
ATCTGTGTGG TACCTTTTAA GGAGCTGGGG CTTGGACTCA GTGAAGACCA GATCTCAGAA 
GAGGAAGCTC GTAACCTGAC AGATGGCTTC AGCCTGCCTG CCTTGAAGGT TTTGTTCCAG 
CTCTGGGTGG CACAGAGTTC AGAGTTCTTC AGACGCCTGG CCCTGTTACT TTCTACAGCG 
AATTCGCCTT CAGGGCCCTT ACTCACTGCT GCACTTCTGC CTCATCATAT CCTCTGTGAC 
GTGACTCAAG GTCTACCTCA TGCTCACTCT GCCTGCTTGG ATGAGCTGAA GCGCAGCTAT 
GAGTTCTTCC GGTACTTTGA AACTCAGCAC CAGTCAGTCC CCCAGCGCTT ATCCAAGACT 
CCGCAGAAGT CGAGGGAGCT GAGCAACGTC CACACAGCAG TCCGTAGCTT ACAGCTCCAT 
CTGAAAGCAC TACTGAATGA AGTAATAATT CTTGAAGATG AACTTGAAAA GCTCGTTTGT 
ACTAAAGAAA CACAAGAGCT GCTATCGGAG GCTTATCCGA TACTAGAGCA GAAATTAAAA 
CTGATTGAAC CACACGTTCA GGCCAGCAAC AGTTGCTGGG AGGAAGCCAT TTCTCAAGTG 
GATAAACTGC TCCGGAGGAA CACAGATAAA AAAGGCAAGC CTGGAGTAGC CTGTGAGAAC 
CCACACTGCA CCGCGGAGCC CTTGGTGCGG CCAGCTCTAC ACATTGAGGA CAGAGACCCC 
ATCCCTGAGG AGCAGGAATT AGAAGCTTAT GTGGATGACA TAGACATAGA AAGTGAGTTC 
CGAAAGGACG ATTTCTATCA CTTGTCTCAG GAAGACAGAG AGAGACAGAA GCGCGAGCAG 
GAGGAGTCCA GGCGGGTGCT GCAGGAGCTG AAGTCTGTGC TGGGATTTAA GGCTTCCGAG 
GCTGAGAGGC AGAAGTGGAA GCAGCTGCTG TTCAGTGACC ACGCTGTGTT GACATCCTTG 
TCTCCTGTAG ACCCAGTGGA ATCTGTAAGT AACTCAGAAC CACCCATGAA TTCAGATACA 
GAAAAAGTCA ATAGTAATGC TACTGAAGAG GAGACTAGTA AGCCCTGTGC CGGCGACAAG 
GAAGACAGTA GGACTGAGTA TGTGTGTGAC AGCCCAACAG AGGGGCCAAG CAAAGACACC 
TCTGCAGACA CAGGCCTCCT CCTTCCAGGA GCCGAGGAAA CCATGTGCCA CCAGCATGAG 
AGTGAGGCTA AGTCTCCTCA GGCTGCTGCT GCGGGGGCCA CTGCCCCTCC GACTCCCAGG 
GACACACTGC AACTCTCCAT TAAGCAGAGG CTGGCTCGAC TCCAGTTGCC ACCAGAATTC 
ACCTTCTCTG CTGGCCTGGC TGCAGAAGTG GCCGCCCGGT CCCTGTCCTT TACCACCATG 
CAGGAACAAA CTTTTGGCGA CGAGGAGGAA GAACAGCTAG TTGAAGGCGG TGAAAATGAG 
GTGGAGGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vezt ( NM_172538.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766126.2 Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03637
Clone ID Related Accession (Same CDS sequence)	NM_172538.5
Accession Version	NM_172538.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	vezatin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK031848.1, BC056428.1, AK036017.1, AC122901.4, AK048068.1, AK031192.1, AK042209.1 and AW124866.1. On Feb 5, 2015 this sequence version replaced NM_172538.4. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036017.1, BC056428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT GGTCAGTGCT 
GCTTGCTCAT TTAATAAGGC TGCACAGCAT CCCGGCCAGC ATCTCATCGG CCTTCGGAAA 
GCCGTCTACA GGACCGTAAG AGCCAACTTT CAAGCCGCAA GGCTAGCTAC CTTGTACATG 
CTGAAAAACT ACCCCCTGAA CTCTGAGAGT GACAATGTCA CCAACTACAT CTGTGTGGTA 
CCTTTTAAGG AGCTGGGGCT TGGACTCAGT GAAGACCAGA TCTCAGAAGA GGAAGCTCGT 
AACCTGACAG ATGGCTTCAG CCTGCCTGCC TTGAAGGTTT TGTTCCAGCT CTGGGTGGCA 
CAGAGTTCAG AGTTCTTCAG ACGCCTGGCC CTGTTACTTT CTACAGCGAA TTCGCCTTCA 
GGGCCCTTAC TCACTGCTGC ACTTCTGCCT CATCATATCC TCTGTGACGT GACTCAAGGT 
CTACCTCATG CTCACTCTGC CTGCTTGGAT GAGCTGAAGC GCAGCTATGA GTTCTTCCGG 
TACTTTGAAA CTCAGCACCA GTCAGTCCCC CAGCGCTTAT CCAAGACTCC GCAGAAGTCG 
AGGGAGCTGA GCAACGTCCA CACAGCAGTC CGTAGCTTAC AGCTCCATCT GAAAGCACTA 
CTGAATGAAG TAATAATTCT TGAAGATGAA CTTGAAAAGC TCGTTTGTAC TAAAGAAACA 
CAAGAGCTGC TATCGGAGGC TTATCCGATA CTAGAGCAGA AATTAAAACT GATTGAACCA 
CACGTTCAGG CCAGCAACAG TTGCTGGGAG GAAGCCATTT CTCAAGTGGA TAAACTGCTC 
CGGAGGAACA CAGATAAAAA AGGCAAGCCT GGAGTAGCCT GTGAGAACCC ACACTGCACC 
GCGGAGCCCT TGGTGCGGCC AGCTCTACAC ATTGAGGACA GAGACCCCAT CCCTGAGGAG 
CAGGAATTAG AAGCTTATGT GGATGACATA GACATAGAAA GTGAGTTCCG AAAGGACGAT 
TTCTATCACT TGTCTCAGGA AGACAGAGAG AGACAGAAGC GCGAGCAGGA GGAGTCCAGG 
CGGGTGCTGC AGGAGCTGAA GTCTGTGCTG GGATTTAAGG CTTCCGAGGC TGAGAGGCAG 
AAGTGGAAGC AGCTGCTGTT CAGTGACCAC GCTGTGTTGA CATCCTTGTC TCCTGTAGAC 
CCAGTGGAAT CTGTAAGTAA CTCAGAACCA CCCATGAATT CAGATACAGA AAAAGTCAAT 
AGTAATGCTA CTGAAGAGGA GACTAGTAAG CCCTGTGCCG GCGACAAGGA AGACAGTAGG 
ACTGAGTATG TGTGTGACAG CCCAACAGAG GGGCCAAGCA AAGACACCTC TGCAGACACA 
GGCCTCCTCC TTCCAGGAGC CGAGGAAACC ATGTGCCACC AGCATGAGAG TGAGGCTAAG 
TCTCCTCAGG CTGCTGCTGC GGGGGCCACT GCCCCTCCGA CTCCCAGGGA CACACTGCAA 
CTCTCCATTA AGCAGAGGCT GGCTCGACTC CAGTTGCCAC CAGAATTCAC CTTCTCTGCT 
GGCCTGGCTG CAGAAGTGGC CGCCCGGTCC CTGTCCTTTA CCACCATGCA GGAACAAACT 
TTTGGCGACG AGGAGGAAGA ACAGCTAGTT GAAGGCGGTG AAAATGAGGT GGAGGAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766126.2
CDS	95..2437
Translation	MTPEFDEEVVFENSPLYQYLQDLGHTDFEICSSSSPKPEKCLTTEGPQPPPTRVLQRQGILLKLTETIKSWTFSSQHSKKDDLLHKLDTGFRLDSLHTILQQEVLLQEDVELLELLDASILSAGQPQQESGHLPTLCSLATPNTWDVSLLFAFISLLIMFPTCWIVSSWLVWGIILFLYLIIRVLKLWRTAKLQMTLKKYRVRLEDMAANSRAFTNLVRKSLRLIQETEVISRGFTLVSAACSFNKAAQHPGQHLIGLRKAVYRTVRANFQAARLATLYMLKNYPLNSESDNVTNYICVVPFKELGLGLSEDQISEEEARNLTDGFSLPALKVLFQLWVAQSSEFFRRLALLLSTANSPSGPLLTAALLPHHILCDVTQGLPHAHSACLDELKRSYEFFRYFETQHQSVPQRLSKTPQKSRELSNVHTAVRSLQLHLKALLNEVIILEDELEKLVCTKETQELLSEAYPILEQKLKLIEPHVQASNSCWEEAISQVDKLLRRNTDKKGKPGVACENPHCTAEPLVRPALHIEDRDPIPEEQELEAYVDDIDIESEFRKDDFYHLSQEDRERQKREQEESRRVLQELKSVLGFKASEAERQKWKQLLFSDHAVLTSLSPVDPVESVSNSEPPMNSDTEKVNSNATEEETSKPCAGDKEDSRTEYVCDSPTEGPSKDTSADTGLLLPGAEETMCHQHESEAKSPQAAAAGATAPPTPRDTLQLSIKQRLARLQLPPEFTFSAGLAAEVAARSLSFTTMQEQTFGDEEEEQLVEGGENEVEEK

Target ORF information:

RefSeq Version	NM_172538.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus vezatin, adherens junctions transmembrane protein (Vezt), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172538.5

ORF Insert Sequence:

ATGACACCGG AGTTTGACGA AGAGGTGGTT TTTGAGAATT CTCCACTTTA CCAGTACCTA 
CAGGATTTGG GACACACAGA TTTTGAGATA TGTTCTTCTT CGTCACCAAA ACCAGAAAAA 
TGCTTGACAA CAGAGGGACC TCAGCCGCCT CCCACCAGAG TCCTACAGAG ACAAGGCATC 
CTGTTGAAAC TGACAGAAAC CATCAAGAGC TGGACGTTTT CCTCTCAGCA CAGTAAGAAA 
GATGACTTAC TCCATAAGTT GGACACCGGG TTTCGGCTGG ACTCACTGCA TACCATCCTG 
CAGCAGGAAG TCCTGCTGCA AGAGGATGTG GAGCTGCTCG AGCTGCTTGA CGCCAGCATC 
CTGTCTGCGG GGCAGCCTCA ACAGGAAAGT GGACACCTTC CAACGCTCTG CTCCCTGGCA 
ACCCCGAACA CTTGGGATGT CTCGCTGCTG TTTGCCTTCA TTAGTTTGCT CATCATGTTT 
CCCACGTGCT GGATTGTGTC CTCCTGGCTG GTGTGGGGAA TAATTCTATT TCTTTACTTG 
ATCATACGAG TTTTGAAATT ATGGAGGACA GCCAAACTGC AAATGACTCT GAAAAAATAT 
AGGGTACGTT TGGAAGACAT GGCAGCCAAT AGCCGAGCCT TCACCAACCT TGTGAGAAAA 
TCACTGCGTC TCATCCAAGA AACTGAAGTC ATTTCCAGGG GATTTACTCT GGTCAGTGCT 
GCTTGCTCAT TTAATAAGGC TGCACAGCAT CCCGGCCAGC ATCTCATCGG CCTTCGGAAA 
GCCGTCTACA GGACCGTAAG AGCCAACTTT CAAGCCGCAA GGCTAGCTAC CTTGTACATG 
CTGAAAAACT ACCCCCTGAA CTCTGAGAGT GACAATGTCA CCAACTACAT CTGTGTGGTA 
CCTTTTAAGG AGCTGGGGCT TGGACTCAGT GAAGACCAGA TCTCAGAAGA GGAAGCTCGT 
AACCTGACAG ATGGCTTCAG CCTGCCTGCC TTGAAGGTTT TGTTCCAGCT CTGGGTGGCA 
CAGAGTTCAG AGTTCTTCAG ACGCCTGGCC CTGTTACTTT CTACAGCGAA TTCGCCTTCA 
GGGCCCTTAC TCACTGCTGC ACTTCTGCCT CATCATATCC TCTGTGACGT GACTCAAGGT 
CTACCTCATG CTCACTCTGC CTGCTTGGAT GAGCTGAAGC GCAGCTATGA GTTCTTCCGG 
TACTTTGAAA CTCAGCACCA GTCAGTCCCC CAGCGCTTAT CCAAGACTCC GCAGAAGTCG 
AGGGAGCTGA GCAACGTCCA CACAGCAGTC CGTAGCTTAC AGCTCCATCT GAAAGCACTA 
CTGAATGAAG TAATAATTCT TGAAGATGAA CTTGAAAAGC TCGTTTGTAC TAAAGAAACA 
CAAGAGCTGC TATCGGAGGC TTATCCGATA CTAGAGCAGA AATTAAAACT GATTGAACCA 
CACGTTCAGG CCAGCAACAG TTGCTGGGAG GAAGCCATTT CTCAAGTGGA TAAACTGCTC 
CGGAGGAACA CAGATAAAAA AGGCAAGCCT GGAGTAGCCT GTGAGAACCC ACACTGCACC 
GCGGAGCCCT TGGTGCGGCC AGCTCTACAC ATTGAGGACA GAGACCCCAT CCCTGAGGAG 
CAGGAATTAG AAGCTTATGT GGATGACATA GACATAGAAA GTGAGTTCCG AAAGGACGAT 
TTCTATCACT TGTCTCAGGA AGACAGAGAG AGACAGAAGC GCGAGCAGGA GGAGTCCAGG 
CGGGTGCTGC AGGAGCTGAA GTCTGTGCTG GGATTTAAGG CTTCCGAGGC TGAGAGGCAG 
AAGTGGAAGC AGCTGCTGTT CAGTGACCAC GCTGTGTTGA CATCCTTGTC TCCTGTAGAC 
CCAGTGGAAT CTGTAAGTAA CTCAGAACCA CCCATGAATT CAGATACAGA AAAAGTCAAT 
AGTAATGCTA CTGAAGAGGA GACTAGTAAG CCCTGTGCCG GCGACAAGGA AGACAGTAGG 
ACTGAGTATG TGTGTGACAG CCCAACAGAG GGGCCAAGCA AAGACACCTC TGCAGACACA 
GGCCTCCTCC TTCCAGGAGC CGAGGAAACC ATGTGCCACC AGCATGAGAG TGAGGCTAAG 
TCTCCTCAGG CTGCTGCTGC GGGGGCCACT GCCCCTCCGA CTCCCAGGGA CACACTGCAA 
CTCTCCATTA AGCAGAGGCT GGCTCGACTC CAGTTGCCAC CAGAATTCAC CTTCTCTGCT 
GGCCTGGCTG CAGAAGTGGC CGCCCGGTCC CTGTCCTTTA CCACCATGCA GGAACAAACT 
TTTGGCGACG AGGAGGAAGA ACAGCTAGTT GAAGGCGGTG AAAATGAGGT GGAGGAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(26)9007-22(2012 Jun)
Danglot L,Freret T,Le Roux N,Narboux N?me N,Burgo A,Hyenne V,Roumier A,Contremoulins V,Dauphin F,Bizot JC,Vodjdani G,Gaspar P,Boulouard M,Poncer JC,Galli T,Simmler MC

Vezatin, a potential target for ADP-ribosylation factor 6, regulates the dendritic formation of hippocampal neurons.
Neuroscience research67(2)126-36(2010 Jun)
Sanda M,Ohara N,Kamata A,Hara Y,Tamaki H,Sukegawa J,Yanagisawa T,Fukunaga K,Kondo H,Sakagami H

Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.
EMBO molecular medicine1(2)125-38(2009 May)
Bahloul A,Simmler MC,Michel V,Leibovici M,Perfettini I,Roux I,Weil D,Nouaille S,Zuo J,Zadro C,Licastro D,Gasparini P,Avan P,Hardelin JP,Petit C

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Vezt cDNA ORF clone, Mus musculus(house mouse)

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