Sprn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sprn
Entrez Gene ID	212518
Full Name	shadow of prion protein
Synonyms	C630041J07
General protein information	Preferred Names shadow of prion protein Names shadow of prion protein Shadoo protein shadoo
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_183147.2	NP_898970.1	shadow of prion protein precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPRN	NP_001012526.2
Pan troglodytes (chimpanzee)	SPRN	XP_001146049.1
	SPRN	XP_001114713.1
Canis lupus familiaris (dog)	LOC100855950	XP_005637973.1
Bos taurus (cattle)	SPRN	NP_001073790.1
Rattus norvegicus (Norway rat)	Sprn	NP_001027015.1
Mus musculus (house mouse)	Sprn	NP_898970.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein.
Nyeste A, Bencsura P, Vida I, Hegyi Z, Homolya L, Fodor E, Welker E
The Journal of biological chemistry291(9)4473-86(2016 Feb)

Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.
Ciric D, Richard CA, Moudjou M, Chapuis J, Sibille P, Daude N, Westaway D, Adrover M, B?ringue V, Martin D, Rezaei H
Journal of virology89(12)6287-93(2015 Jun)

Endoproteolytic processing of the mammalian prion glycoprotein family.
Mays CE, Coomaraswamy J, Watts JC, Yang J, Ko KW, Strome B, Mercer RC, Wohlgemuth SL, Schmitt-Ulms G, Westaway D
The FEBS journal281(3)862-76(2014 Feb)

Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie.
Li S, Ju C, Han C, Li Z, Liu W, Ye X, Xu J, Xulong L, Wang X, Chen Z, Meng K, Wan J
Prion8(5)339-43(2014)

Shadoo binds lipid membranes and undergoes aggregation and fibrillization.
Li Q, Chevalier C, Henry C, Richard CA, Moudjou M, Vidic J
Biochemical and biophysical research communications438(3)519-25(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that expression of shadoo in neuron or hepatocyte cell lines induces same toxic phenotype of drug hypersensitivity as prion protein/PrP(-central region); this effect is counteracted by co-expression of PrP-wild-type.
Title: Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein.

These observations suggest that Shadoo can affect the prion replication process by (i) acting as a holdase and (ii) interfering with the dominant-negative inhibitor effect of the C1 fragment.
Title: Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.

Data suggest that glycosylation status of the prion protein and yet-to-be-dentified proteases modulate internal C1 and C2 endoproteolysis of Shadoo (shadow of prion protein) and Doppel (doppelganger prion) in mouse neurons.
Title: Endoproteolytic processing of the mammalian prion glycoprotein family.

No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice
Title: Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie.

binding of Sho to anionic liposomes has disruptive effect on integrity of lipid bilayer leading to formation of supramolecular lipid-protein complexes. prion diseases might depend on the oligomerization state of Sho and on these lipoprotein assembles.
Title: Shadoo binds lipid membranes and undergoes aggregation and fibrillization.

The following Sprn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sprn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17683	NM_183147.2 Latest version!	Mus musculus shadow of prion protein (Sprn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sprn ( NM_183147.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_898970.1 Mus musculus shadow of prion protein (Sprn), mRNA.

Price & Availability↑

CloneID	OMu17683
Clone ID Related Accession (Same CDS sequence)	NM_183147.2
Accession Version	NM_183147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	shadow of prion protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056484.1. On Nov 29, 2004 this sequence version replaced NM_183147.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056484.1, SRR1660815.203964.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGAACTGGA CTGCTGCCAC GTGCTGGGCT CTGCTGCTGG CCGCCGCCTT CCTCTGTGAC 
AGCTGTTCCG CCAAGGGCGG CCGCGGAGGC GCTCGAGGCA GTGCCCGGGG GGTGCGCGGA 
GGTGCACGCG GGGCATCAAG AGTACGCGTA AGGCCGGCGC CCCGCTACGG CTCCTCTCTG 
CGCGTGGCGG CTGCAGGGGC AGCAGCAGGG GCTGCAGCGG GTGTGGCTGC GGGCCTTGCT 
ACCGGCTCTG GCTGGAGGAG GACCTCAGGG CCTGGAGAGC TAGGCCTGGA GGACGATGAG 
AATGGGGCAA TGGGAGGCAA CGGAACCGAC CGAGGAGTCT ACAGCTACTG GGCCTGGACT 
TCCGGCTCAG GGTCCGTGCA CAGCCCACGT ATTTGTCTGC TTCTGGGCGG CACCCTTGGT 
GCCCTAGAAC TGCTTCGGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_898970.1
CDS	127..570
Translation	MNWTAATCWALLLAAAFLCDSCSAKGGRGGARGSARGVRGGARGASRVRVRPAPRYGSSLRVAAAGAAAGAAAGVAAGLATGSGWRRTSGPGELGLEDDENGAMGGNGTDRGVYSYWAWTSGSGSVHSPRICLLLGGTLGALELLRP

Target ORF information:

RefSeq Version	NM_183147.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus shadow of prion protein (Sprn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183147.2

ORF Insert Sequence:

ATGAACTGGA CTGCTGCCAC GTGCTGGGCT CTGCTGCTGG CCGCCGCCTT CCTCTGTGAC 
AGCTGTTCCG CCAAGGGCGG CCGCGGAGGC GCTCGAGGCA GTGCCCGGGG GGTGCGCGGA 
GGTGCACGCG GGGCATCAAG AGTACGCGTA AGGCCGGCGC CCCGCTACGG CTCCTCTCTG 
CGCGTGGCGG CTGCAGGGGC AGCAGCAGGG GCTGCAGCGG GTGTGGCTGC GGGCCTTGCT 
ACCGGCTCTG GCTGGAGGAG GACCTCAGGG CCTGGAGAGC TAGGCCTGGA GGACGATGAG 
AATGGGGCAA TGGGAGGCAA CGGAACCGAC CGAGGAGTCT ACAGCTACTG GGCCTGGACT 
TCCGGCTCAG GGTCCGTGCA CAGCCCACGT ATTTGTCTGC TTCTGGGCGG CACCCTTGGT 
GCCCTAGAAC TGCTTCGGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein.
The Journal of biological chemistry291(9)4473-86(2016 Feb)
Nyeste A,Bencsura P,Vida I,Hegyi Z,Homolya L,Fodor E,Welker E

Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.
Journal of virology89(12)6287-93(2015 Jun)
Ciric D,Richard CA,Moudjou M,Chapuis J,Sibille P,Daude N,Westaway D,Adrover M,B?ringue V,Martin D,Rezaei H

Endoproteolytic processing of the mammalian prion glycoprotein family.
The FEBS journal281(3)862-76(2014 Feb)
Mays CE,Coomaraswamy J,Watts JC,Yang J,Ko KW,Strome B,Mercer RC,Wohlgemuth SL,Schmitt-Ulms G,Westaway D

Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie.
Prion8(5)339-43(2014)
Li S,Ju C,Han C,Li Z,Liu W,Ye X,Xu J,Xulong L,Wang X,Chen Z,Meng K,Wan J

Shadoo binds lipid membranes and undergoes aggregation and fibrillization.
Biochemical and biophysical research communications438(3)519-25(2013 Aug)
Li Q,Chevalier C,Henry C,Richard CA,Moudjou M,Vidic J

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Sprn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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