Arfgef1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arfgef1
Entrez Gene ID	211673
Full Name	ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
Synonyms	ARFGEP1,BIG1,D130059B05Rik,D730028O18Rik,P200
General protein information	Preferred Names ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 ADP-ribosylation factor guanine nucleotide-exchange factor 1 brefeldin A-inhibited GEP 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102430.1	NP_001095900.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain.
Teoh JJ, Iwano T, Kunii M, Atik N, Avriyanti E, Yoshimura SI, Moriwaki K, Harada A
PloS one12(4)e0175888(2017)

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Steinmetz CC, Tatavarty V, Sugino K, Shima Y, Joseph A, Lin H, Rutlin M, Lambo M, Hempel CM, Okaty BW, Paradis S, Nelson SB, Turrigiano GG
Cell reports16(10)2711-2722(2016 09)

The cortical hem regulates the size and patterning of neocortex.
Caronia-Brown G, Yoshida M, Gulden F, Assimacopoulos S, Grove EA
Development (Cambridge, England)141(14)2855-65(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an early acting GEF (GBF1) activates ARFs that mediate recruitment of late acting GEFs (BIG1/2) to coordinate coating events within the pre-Golgi/Golgi/TGN continuum.
Title: The Sec7 guanine nucleotide exchange factor GBF1 regulates membrane recruitment of BIG1 and BIG2 guanine nucleotide exchange factors to the trans-Golgi network (TGN).

The following Arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13631	NM_001102430.1 Latest version!	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1357.30 $1939.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arfgef1 ( NM_001102430.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001095900.1 Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), mRNA.

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CloneID	OMu13631
Clone ID Related Accession (Same CDS sequence)	NM_001102430.1
Accession Version	NM_001102430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC102462.19 and AC159972.5. On Aug 23, 2007 this sequence version replaced XM_975420.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTCTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCC 
CTAGAGGAAA TAAAAGTTGA AACTGAGAAA CAAAGCCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAGGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTTTTACCCT TTGAGTTGGC ATGCCAGTCC AAATGTCCCC GCATAGTTAG TACATCTCTA 
GATTGTTTGC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAGGGCTCC AGATAGTACG 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTCCAAGGT 
CCTCAAACAG ATGAAGGAGT TCAGTTGCAG ATAATAAAGG CTTTACTTAC CGCAGTGACA 
TCACAACACA TAGAAATCCA TGAAGGAACT GTGCTGCAAG CTGTGAGGAC ATGCTACAAT 
ATCTATTTAG CCAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAA 
ATGCTGAACG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAGGC CAAACAAATG 
GAAAGAGAAA GACATCGGCA GCAGCAGCAT TTGTTACAGT CTCCAGTAAG CCATCATGAG 
CCCGAGTCAC CTCATCTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT AAACCAAGAG 
CATGAAGGGG ATCTTGAGCC CCAAACACAT GATGTGGATA AAAGTCTCCA AGATGATACA 
GAGCCTGAAA ACGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACAGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GATATCTTGT ATGATGGAGA CTATGAAGAA 
AAGCCACTTG ATATTGTCCA GAGCATTGTA GAAGAAATGG TGAACATCAT TGTTGGAGAC 
ATGGGAGAAG GGATGGCTAT CAGTGCAAGT ACAGAAGGCA ACACTGGAAC TGTAGAAGAT 
GGTAGTGACA GTGAAAACAT CCAAGCAAAT GGAATCCCAG GAACACCCAT TTCTGTTGCA 
TATACACCAT CGTTACCTGA CGACAGGTTA TCTGTCTCCT CCAATGATAC TCAGGAATCT 
GGAAATTCTT CAGGACCTTC ACCTGGTGCT AAGTTTTCCC ACATTTTACA AAAGGATGCC 
TTTCTAGTGT TCAGGTCATT GTGTAAGCTG TCAATGAAAC CACTGTCAGA TGGACCACCA 
GATCCAAAGT CTCATGAGTT ACGGTCCAAG ATTCTTTCAT TGCAGTTGCT TCTGTCCATT 
CTGCAGAATG CAGGACCTGT TTTCAGGACA AATGAGATGT TTATTAATGC TATCAAGCAG 
TATCTATGTG TTGCACTCTC AAAAAACGGA GTCTCATCTG TTCCAGAGGT TTTTGAGCTT 
TCTCTTTCCA TATTTCTTAC TTTGTTGTCA AACTTCAAGA CACATCTGAA GATGCAAATT 
GAGGTATTCT TTAAAGAAAT CTTCCTGTAC ATTTTGGAAA CGTCTACAAG CTCATTTGAC 
CATAAGTGGA TGGTTATTCA GACACTGACA AGGATCTGTG CAGATGCTCA GAGTGTGGTG 
GACATTTATG TAAATTATGA CTGTGACCTA AATGCAGCAA ATATATTTGA AAGACTAGTA 
AATGACCTGT CGAAAATTGC CCAAGGAAGG GGCAGCCAAG AACTTGGTAT GAGTAATGTT 
CAGGAATTGA GCCTGCGAAA AAAGGGGTTA GAATGTTTAG TTTCAATTTT GAAATGTATG 
GTTGAATGGA GCAAGGATCA GTATGTGAAC CCCAACTCTC AAACTACCCT TGGTCAGGAA 
AAACCCTCAG AGCAAGAGAT AAGTGAAGTT AAACACCCAG AGACTATAAA CAGATATGGA 
AGTTTAAACT CCTTAGAATC CACATCATCA TCAGGAATTG GCAGCTACAG TACACAGATG 
TCTGGCACTG ATAATCCAGA ACAATTTGAG GTCCTAAAGC AGCAAAAAGA AATAATAGAA 
CAAGGGATTG ATTTATTTAA TAAGAAACCA AAGAGAGGAA TACAATACCT CCAAGAACAA 
GGGATGCTTG GCACCACACC CGAAGATATA GCTCAGTTCT TACATCAAGA AGAAAGATTA 
GACTCTACTC AGGTGGGTGA GTTCCTGGGA GATAATGATA AATTCAATAA AGAAGTCATG 
TATGCATATG TAGACCAACA TGACTTTTCA GGAAAAGACT TTGTTTCAGC TCTTCGTCTA 
TTTCTGGAAG GATTCCGGCT TCCAGGAGAA GCTCAGAAAA TTGATCGATT AATGGAAAAA 
TTTGCTGCAA GATACCTAGA GTGCAACCAA GGACAAACCC TTTTTGCTAG TGCAGATACT 
GCTTATGTTT TGGCCTACTC AATTATCATG TTGACTACAG ACCTTCACAG TCCACAGGTT 
AAAAACAAAA TGACAAAAGA ACAGTACATT AAAATGAATC GTGGTATCAA TGACAGTAAA 
GATCTCCCTG AAGAGTATCT ATCAGCCATT TATAACGAAA TAGCTGGGAA AAAAATATCA 
ATGAAAGAAA CAAAAGAACT AACAATCCCT ACAAAGTCAA CTAAACAGAA TGTAGCCAGT 
GAAAAGCAAA GACGACTTCT GTATAACTTA GAAATGGAAC AGATGGCCAA GACGGCTAAA 
GCACTCATGG AAGCTGTGAG CCATGTTCAG GCACCTTTTA CAAGTGCAAC ACACTTGGAG 
CACGTGAGGC CGATGTTTAA GTTGGCTTGG ACACCTTTTC TGGCTGCATT CAGTGTGGGT 
CTCCAAGACT GTGATGATAC TGAAGTAGCC TCTCTTTGCC TAGAAGGGAT AAGATGTGCT 
ATCAGGATTG CATGCATTTT CAGCATTCAG CTGGAAAGAG ATGCATATGT CCAAGCACTA 
GCCAGATTTA CCTTACTTAC AGTGAGTTCT GGTATTACTG AAATGAAACA GAAGAATATC 
GACACAATAA AAACACTCAT CACGGTGGCT CACACTGATG GAAATTATTT AGGAAATTCG 
TGGCATGAGA TCCTGAAGTG CATCAGTCAG TTAGAGCTGG CACAACTTAT AGGAACTGGA 
GTGAAGCCTC GGTACATTTC TGGGACTGTG AGAGGCAGAG AAGGGTCTCT CACTGGGACA 
AAAGATCAGG CTCCAGATGA ATTTGTTGGT CTAGGGCTAG TTGGAGGAAA TGTGGACTGG 
AAGCAGATAG CCAGTATTCA GGAGTCTATT GGAGAAACCA GCTCTCAAAG TGTGGTAGTT 
GCTGTGGATA GAATATTCAC CGGATCCACA AGGCTAGATG GAAATGCTAT TGTTGATTTT 
GTCCGTTGGC TATGTGCTGT GTCTATGGAT GAATTGCTTT CTACTACACA CCCAAGAATG 
TTTAGTCTAC AAAAAATAGT AGAAATATCA TACTATAACA TGGGAAGAAT AAGACTGCAG 
TGGTCTCGGA TTTGGGAAGT TATTGGAGAT CATTTTAATA AGGTTGGTTG CAATCCTAAT 
GAAGATGTAG CCATATTTGC AGTAGACTCT CTGAGGCAGC TATCAATGAA GTTTTTAGAG 
AAAGGAGAGC TTGCAAACTT CAGATTCCAA AAGGATTTCT TACGACCTTT TGAACATATA 
ATGAAACGAA ACAGGTCTCC AACAATTCGA GATATGGTTG TACGGTGTAT AGCACAAATG 
GTTAATTCTC AGGCTGCAAA TATTCGTTCA GGATGGAAGA ACATTTTCTC AGTATTCCAT 
CTAGCTGCAT CAGACCAAGA TGAAAGCATA GTAGAACTTG CATTTCAGAC AACAGGGCAC 
ATTGTCACTC TTGTATTTGA AAAACACTTT CCGGCCACCA TTGATTCTTT CCAGGATGCA 
GTGAAGTGTT TGTCTGAATT CGCATGTAAT GCAGCTTTCC CAGACACAAG TATGGAAGCA 
ATCCGACTTA TTCGCCATTG TGCAAAATAT GTGTCTGATA GACCGCAGGC TTTCAAGGAG 
TACACAAGTG ATGACATGAG CGTGGCACCT GAAGACAGGG TGTGGGTGAG AGGGTGGTTC 
CCGATCCTCT TTGAGTTGTC TTGTATCATC AATAGATGCA AATTAGATGT AAGAACCAGG 
GGCTTAACAG TAATGTTTGA AATAATGAAA ACATATGGCC ACACTTATGA AAAACATTGG 
TGGCAGGACT TATTCAGAAT TGTTTTCAGA ATCTTTGACA ACATGAAATT GCCAGAACAG 
CAGACAGAGA AAGCTGAATG GATGACAACA ACTTGTAACC ATGCCCTTTA TGCAATATGT 
GATGTATTCA CCCAGTATTT AGAAGTTCTC AGCGATGTAC TTTTGGATGA TATTTTTGCC 
CAGCTTTACT GGTGTGTACA ACAAGACAAT GAGCAGTTAG CACGATCTGG TACAAACTGC 
TTAGAAAACG TGGTTATTCT GAATGGTGAA AAGTTTACTC TTGAAATCTG GGATAAAACG 
TGTAACTGCA CCCTGGATAT CTTCAAAACC ACAATTCCTC ACGCGCTTCT GACTTGGCGT 
CCCACCTCTG GCGAGGCCGA ACCCCCATCT CCATCAGCTG TGAGTGAAAA GCCGTTGGAT 
GCAATATCAC AAAAATCTGT AGACATACAT GATTCTATTC AACCAAGATC TTCAGATAAC 
AGACAGCAAG CACCACTGGT TTCTGTTTCT ACTGTGAGTG AAGAAGTTAG CAAAGTGAAA 
TCCACAGCAA AATTCCCAGA ACAAAAATTG TTTGCTGCCC TGTTGATTAA ATGTGTTGTG 
CAGCTGGAAC TCATCCAGAC CATCGACAAC ATTGTCTTCT TCCCAGCCAC AAGCAAGAAG 
GAGGATGCAG AGAACTTAGC TGCAGCCCAG AGAGATGCAG TAGACTTCGA TGTTCGAGTT 
GATACTCAAG ACCAAGGAAT GTACCGCTTT TTAACATCAC AACAACTGTT CAAGCTACTG 
GACTGCTTAT TAGAGTCGCA TAGATTTGCA AAAGCATTTA ATTCCAACAA TGAACAGAGG 
ACTGCTCTGT GGAAAGCAGG CTTCAAAGGC AAGTCCAAGC CCAATCTTCT AAAACAGGAG 
ACAAGCAGCC TGGCGTGTGG GCTGCGCATT CTCTTCCGGA TGTACATGGA CGAGAGCAGA 
GTCAGTGCCT GGGAAGAAGT CCAGCAGAGG CTTTTAAACG TCTGCAGGGA AGCGCTGAGC 
TACTTCCTCA CTCTAACATC CGAAAGTCAC CGGGAAGCCT GGACTAACTT ACTGTTACTG 
TTCCTCACTA AAGTTCTGAA GATCAGTGAC AGCAGGTTTA AAGCTCACGC ATCATTCTAC 
TACCCTCTCT TATGTGAAAT TATGCAATTT GACTTGATTC CGGAACTGCG TGCTGTTCTT 
AGAAGATTTT TCCTGCGAAT TGGAATAGTA TTTCAAATAT CACAACCACC TGAACAGGAA 
CTTGGGATAA ACAGGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095900.1
CDS	177..5717
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGRAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQQQHLLQSPVSHHEPESPHLRYLPPQTVDHINQEHEGDLEPQTHDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNDILYDGDYEEKPLDIVQSIVEEMVNIIVGDMGEGMAISASTEGNTGTVEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEISEVKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMSVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPTSGEAEPPSPSAVSEKPLDAISQKSVDIHDSIQPRSSDNRQQAPLVSVSTVSEEVSKVKSTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDESRVSAWEEVQQRLLNVCREALSYFLTLTSESHREAWTNLLLLFLTKVLKISDSRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGIVFQISQPPEQELGINRQ

Target ORF information:

RefSeq Version	NM_001102430.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102430.1

ORF Insert Sequence:

ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTCTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCC 
CTAGAGGAAA TAAAAGTTGA AACTGAGAAA CAAAGCCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAGGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTTTTACCCT TTGAGTTGGC ATGCCAGTCC AAATGTCCCC GCATAGTTAG TACATCTCTA 
GATTGTTTGC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAGGGCTCC AGATAGTACG 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTCCAAGGT 
CCTCAAACAG ATGAAGGAGT TCAGTTGCAG ATAATAAAGG CTTTACTTAC CGCAGTGACA 
TCACAACACA TAGAAATCCA TGAAGGAACT GTGCTGCAAG CTGTGAGGAC ATGCTACAAT 
ATCTATTTAG CCAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAA 
ATGCTGAACG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAGGC CAAACAAATG 
GAAAGAGAAA GACATCGGCA GCAGCAGCAT TTGTTACAGT CTCCAGTAAG CCATCATGAG 
CCCGAGTCAC CTCATCTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT AAACCAAGAG 
CATGAAGGGG ATCTTGAGCC CCAAACACAT GATGTGGATA AAAGTCTCCA AGATGATACA 
GAGCCTGAAA ACGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACAGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAAAT GATATCTTGT ATGATGGAGA CTATGAAGAA 
AAGCCACTTG ATATTGTCCA GAGCATTGTA GAAGAAATGG TGAACATCAT TGTTGGAGAC 
ATGGGAGAAG GGATGGCTAT CAGTGCAAGT ACAGAAGGCA ACACTGGAAC TGTAGAAGAT 
GGTAGTGACA GTGAAAACAT CCAAGCAAAT GGAATCCCAG GAACACCCAT TTCTGTTGCA 
TATACACCAT CGTTACCTGA CGACAGGTTA TCTGTCTCCT CCAATGATAC TCAGGAATCT 
GGAAATTCTT CAGGACCTTC ACCTGGTGCT AAGTTTTCCC ACATTTTACA AAAGGATGCC 
TTTCTAGTGT TCAGGTCATT GTGTAAGCTG TCAATGAAAC CACTGTCAGA TGGACCACCA 
GATCCAAAGT CTCATGAGTT ACGGTCCAAG ATTCTTTCAT TGCAGTTGCT TCTGTCCATT 
CTGCAGAATG CAGGACCTGT TTTCAGGACA AATGAGATGT TTATTAATGC TATCAAGCAG 
TATCTATGTG TTGCACTCTC AAAAAACGGA GTCTCATCTG TTCCAGAGGT TTTTGAGCTT 
TCTCTTTCCA TATTTCTTAC TTTGTTGTCA AACTTCAAGA CACATCTGAA GATGCAAATT 
GAGGTATTCT TTAAAGAAAT CTTCCTGTAC ATTTTGGAAA CGTCTACAAG CTCATTTGAC 
CATAAGTGGA TGGTTATTCA GACACTGACA AGGATCTGTG CAGATGCTCA GAGTGTGGTG 
GACATTTATG TAAATTATGA CTGTGACCTA AATGCAGCAA ATATATTTGA AAGACTAGTA 
AATGACCTGT CGAAAATTGC CCAAGGAAGG GGCAGCCAAG AACTTGGTAT GAGTAATGTT 
CAGGAATTGA GCCTGCGAAA AAAGGGGTTA GAATGTTTAG TTTCAATTTT GAAATGTATG 
GTTGAATGGA GCAAGGATCA GTATGTGAAC CCCAACTCTC AAACTACCCT TGGTCAGGAA 
AAACCCTCAG AGCAAGAGAT AAGTGAAGTT AAACACCCAG AGACTATAAA CAGATATGGA 
AGTTTAAACT CCTTAGAATC CACATCATCA TCAGGAATTG GCAGCTACAG TACACAGATG 
TCTGGCACTG ATAATCCAGA ACAATTTGAG GTCCTAAAGC AGCAAAAAGA AATAATAGAA 
CAAGGGATTG ATTTATTTAA TAAGAAACCA AAGAGAGGAA TACAATACCT CCAAGAACAA 
GGGATGCTTG GCACCACACC CGAAGATATA GCTCAGTTCT TACATCAAGA AGAAAGATTA 
GACTCTACTC AGGTGGGTGA GTTCCTGGGA GATAATGATA AATTCAATAA AGAAGTCATG 
TATGCATATG TAGACCAACA TGACTTTTCA GGAAAAGACT TTGTTTCAGC TCTTCGTCTA 
TTTCTGGAAG GATTCCGGCT TCCAGGAGAA GCTCAGAAAA TTGATCGATT AATGGAAAAA 
TTTGCTGCAA GATACCTAGA GTGCAACCAA GGACAAACCC TTTTTGCTAG TGCAGATACT 
GCTTATGTTT TGGCCTACTC AATTATCATG TTGACTACAG ACCTTCACAG TCCACAGGTT 
AAAAACAAAA TGACAAAAGA ACAGTACATT AAAATGAATC GTGGTATCAA TGACAGTAAA 
GATCTCCCTG AAGAGTATCT ATCAGCCATT TATAACGAAA TAGCTGGGAA AAAAATATCA 
ATGAAAGAAA CAAAAGAACT AACAATCCCT ACAAAGTCAA CTAAACAGAA TGTAGCCAGT 
GAAAAGCAAA GACGACTTCT GTATAACTTA GAAATGGAAC AGATGGCCAA GACGGCTAAA 
GCACTCATGG AAGCTGTGAG CCATGTTCAG GCACCTTTTA CAAGTGCAAC ACACTTGGAG 
CACGTGAGGC CGATGTTTAA GTTGGCTTGG ACACCTTTTC TGGCTGCATT CAGTGTGGGT 
CTCCAAGACT GTGATGATAC TGAAGTAGCC TCTCTTTGCC TAGAAGGGAT AAGATGTGCT 
ATCAGGATTG CATGCATTTT CAGCATTCAG CTGGAAAGAG ATGCATATGT CCAAGCACTA 
GCCAGATTTA CCTTACTTAC AGTGAGTTCT GGTATTACTG AAATGAAACA GAAGAATATC 
GACACAATAA AAACACTCAT CACGGTGGCT CACACTGATG GAAATTATTT AGGAAATTCG 
TGGCATGAGA TCCTGAAGTG CATCAGTCAG TTAGAGCTGG CACAACTTAT AGGAACTGGA 
GTGAAGCCTC GGTACATTTC TGGGACTGTG AGAGGCAGAG AAGGGTCTCT CACTGGGACA 
AAAGATCAGG CTCCAGATGA ATTTGTTGGT CTAGGGCTAG TTGGAGGAAA TGTGGACTGG 
AAGCAGATAG CCAGTATTCA GGAGTCTATT GGAGAAACCA GCTCTCAAAG TGTGGTAGTT 
GCTGTGGATA GAATATTCAC CGGATCCACA AGGCTAGATG GAAATGCTAT TGTTGATTTT 
GTCCGTTGGC TATGTGCTGT GTCTATGGAT GAATTGCTTT CTACTACACA CCCAAGAATG 
TTTAGTCTAC AAAAAATAGT AGAAATATCA TACTATAACA TGGGAAGAAT AAGACTGCAG 
TGGTCTCGGA TTTGGGAAGT TATTGGAGAT CATTTTAATA AGGTTGGTTG CAATCCTAAT 
GAAGATGTAG CCATATTTGC AGTAGACTCT CTGAGGCAGC TATCAATGAA GTTTTTAGAG 
AAAGGAGAGC TTGCAAACTT CAGATTCCAA AAGGATTTCT TACGACCTTT TGAACATATA 
ATGAAACGAA ACAGGTCTCC AACAATTCGA GATATGGTTG TACGGTGTAT AGCACAAATG 
GTTAATTCTC AGGCTGCAAA TATTCGTTCA GGATGGAAGA ACATTTTCTC AGTATTCCAT 
CTAGCTGCAT CAGACCAAGA TGAAAGCATA GTAGAACTTG CATTTCAGAC AACAGGGCAC 
ATTGTCACTC TTGTATTTGA AAAACACTTT CCGGCCACCA TTGATTCTTT CCAGGATGCA 
GTGAAGTGTT TGTCTGAATT CGCATGTAAT GCAGCTTTCC CAGACACAAG TATGGAAGCA 
ATCCGACTTA TTCGCCATTG TGCAAAATAT GTGTCTGATA GACCGCAGGC TTTCAAGGAG 
TACACAAGTG ATGACATGAG CGTGGCACCT GAAGACAGGG TGTGGGTGAG AGGGTGGTTC 
CCGATCCTCT TTGAGTTGTC TTGTATCATC AATAGATGCA AATTAGATGT AAGAACCAGG 
GGCTTAACAG TAATGTTTGA AATAATGAAA ACATATGGCC ACACTTATGA AAAACATTGG 
TGGCAGGACT TATTCAGAAT TGTTTTCAGA ATCTTTGACA ACATGAAATT GCCAGAACAG 
CAGACAGAGA AAGCTGAATG GATGACAACA ACTTGTAACC ATGCCCTTTA TGCAATATGT 
GATGTATTCA CCCAGTATTT AGAAGTTCTC AGCGATGTAC TTTTGGATGA TATTTTTGCC 
CAGCTTTACT GGTGTGTACA ACAAGACAAT GAGCAGTTAG CACGATCTGG TACAAACTGC 
TTAGAAAACG TGGTTATTCT GAATGGTGAA AAGTTTACTC TTGAAATCTG GGATAAAACG 
TGTAACTGCA CCCTGGATAT CTTCAAAACC ACAATTCCTC ACGCGCTTCT GACTTGGCGT 
CCCACCTCTG GCGAGGCCGA ACCCCCATCT CCATCAGCTG TGAGTGAAAA GCCGTTGGAT 
GCAATATCAC AAAAATCTGT AGACATACAT GATTCTATTC AACCAAGATC TTCAGATAAC 
AGACAGCAAG CACCACTGGT TTCTGTTTCT ACTGTGAGTG AAGAAGTTAG CAAAGTGAAA 
TCCACAGCAA AATTCCCAGA ACAAAAATTG TTTGCTGCCC TGTTGATTAA ATGTGTTGTG 
CAGCTGGAAC TCATCCAGAC CATCGACAAC ATTGTCTTCT TCCCAGCCAC AAGCAAGAAG 
GAGGATGCAG AGAACTTAGC TGCAGCCCAG AGAGATGCAG TAGACTTCGA TGTTCGAGTT 
GATACTCAAG ACCAAGGAAT GTACCGCTTT TTAACATCAC AACAACTGTT CAAGCTACTG 
GACTGCTTAT TAGAGTCGCA TAGATTTGCA AAAGCATTTA ATTCCAACAA TGAACAGAGG 
ACTGCTCTGT GGAAAGCAGG CTTCAAAGGC AAGTCCAAGC CCAATCTTCT AAAACAGGAG 
ACAAGCAGCC TGGCGTGTGG GCTGCGCATT CTCTTCCGGA TGTACATGGA CGAGAGCAGA 
GTCAGTGCCT GGGAAGAAGT CCAGCAGAGG CTTTTAAACG TCTGCAGGGA AGCGCTGAGC 
TACTTCCTCA CTCTAACATC CGAAAGTCAC CGGGAAGCCT GGACTAACTT ACTGTTACTG 
TTCCTCACTA AAGTTCTGAA GATCAGTGAC AGCAGGTTTA AAGCTCACGC ATCATTCTAC 
TACCCTCTCT TATGTGAAAT TATGCAATTT GACTTGATTC CGGAACTGCG TGCTGTTCTT 
AGAAGATTTT TCCTGCGAAT TGGAATAGTA TTTCAAATAT CACAACCACC TGAACAGGAA 
CTTGGGATAA ACAGGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain.
PloS one12(4)e0175888(2017)
Teoh JJ,Iwano T,Kunii M,Atik N,Avriyanti E,Yoshimura SI,Moriwaki K,Harada A

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Cell reports16(10)2711-2722(2016 09)
Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

The cortical hem regulates the size and patterning of neocortex.
Development (Cambridge, England)141(14)2855-65(2014 Jul)
Caronia-Brown G,Yoshida M,Gulden F,Assimacopoulos S,Grove EA

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Arfgef1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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