Thsd4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Thsd4
Entrez Gene ID	207596
Full Name	thrombospondin, type I, domain containing 4
Synonyms	ADAMTSL-6,ADAMTSL6,AI585901,Adamtsl4,B230114P05Rik
General protein information	Preferred Names thrombospondin, type I, domain containing 4 Names thrombospondin type-1 domain-containing protein 4 A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6 ADAMTS-like protein 4 ADAMTS-like protein 6 ADAMTSL-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510905.4	XP_006510968.1	thrombospondin type-1 domain-containing protein 4 isoform X1
NM_001040426.3	NP_001035516.2	thrombospondin type-1 domain-containing protein 4 isoform a precursor
XM_006510905.3	XP_006510968.1	thrombospondin type-1 domain-containing protein 4 isoform X1
XM_006510906.3	XP_006510969.1	thrombospondin type-1 domain-containing protein 4 isoform X2
XM_006510906.4	XP_006510969.1	thrombospondin type-1 domain-containing protein 4 isoform X2
NM_172444.4	NP_766032.1	thrombospondin type-1 domain-containing protein 4 isoform b precursor
XM_006510907.3	XP_006510970.1	thrombospondin type-1 domain-containing protein 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THSD4	XP_523113.2
Canis lupus familiaris (dog)	THSD4	XP_005638767.1
Xenopus tropicalis (tropical clawed frog)	thsd4	XP_002937716.2
Danio rerio (zebrafish)	adamtsl7	XP_684575.2
Mus musculus (house mouse)	Thsd4	NP_001035516.2
Gallus gallus (chicken)	THSD4	XP_413780.3
Homo sapiens (human)	THSD4	NP_079093.2

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac physiologic and genetic predictors of hyperoxia-induced acute lung injury in mice.
Howden R, Cho HY, Miller-DeGraff L, Walker C, Clark JA, Myers PH, Rouse DC, Kleeberger SR
American journal of respiratory cell and molecular biology46(4)470-8(2012 Apr)

ADAMTSL6? protein rescues fibrillin-1 microfibril disorder in a Marfan syndrome mouse model through the promotion of fibrillin-1 assembly.
Saito M, Kurokawa M, Oda M, Oshima M, Tsutsui K, Kosaka K, Nakao K, Ogawa M, Manabe R, Suda N, Ganjargal G, Hada Y, Noguchi T, Teranaka T, Sekiguchi K, Yoneda T, Tsuji T
The Journal of biological chemistry286(44)38602-13(2011 Nov)

Altered gene expression and spongiotrophoblast differentiation in placenta from a mouse model of diabetes in pregnancy.
Salbaum JM, Kruger C, Zhang X, Delahaye NA, Pavlinkova G, Burk DH, Kappen C
Diabetologia54(7)1909-20(2011 Jul)

O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.
Du J, Takeuchi H, Leonhard-Melief C, Shroyer KR, Dlugosz M, Haltiwanger RS, Holdener BC
Developmental biology346(1)25-38(2010 Oct)

A genomic atlas of mouse hypothalamic development.
Shimogori T, Lee DA, Miranda-Angulo A, Yang Y, Wang H, Jiang L, Yoshida AC, Kataoka A, Mashiko H, Avetisyan M, Qi L, Qian J, Blackshaw S
Nature neuroscience13(6)767-75(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ADAMTSL6beta has a role in fibrillin-1 microfibril formation
Title: ADAMTSL6? protein rescues fibrillin-1 microfibril disorder in a Marfan syndrome mouse model through the promotion of fibrillin-1 assembly.

Results indicated that ADAMTSL-6 is a novel microfibril-associated protein that binds directly to fibrillin-1 and promotes fibrillin-1 matrix assembly.
Title: ADAMTSL-6 is a novel extracellular matrix protein that binds to fibrillin-1 and promotes fibrillin-1 fibril formation.

The following Thsd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thsd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03458	NM_172444.4 Latest version!	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu00834	XM_006510906.4 Latest version!	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu42620	XM_006510905.4 Latest version!	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu00834	NM_001040426.3 Latest version!	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu00834	XM_006510907.3 Latest version!	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu42620	XM_006510905.3	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu00834	XM_006510906.3	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Thsd4 ( NM_172444.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766032.1 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu03458
Clone ID Related Accession (Same CDS sequence)	NM_172444.4
Accession Version	NM_172444.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CV559893.1, AK045414.1 and AC131749.2. On Jan 6, 2010 this sequence version replaced NM_172444.3. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK045414.1, BC139329.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGTCA GCTACCTGTT ATTAACATTG TTCCACACTG AGACAGCAAT GTTAGCAAGA 
CCTGGGGGAG AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766032.1
CDS	163..2139
Translation	MFVSYLLLTLFHTETAMLARPGGESIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	NM_172444.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172444.4

ORF Insert Sequence:

ATGTTTGTCA GCTACCTGTT ATTAACATTG TTCCACACTG AGACAGCAAT GTTAGCAAGA 
CCTGGGGGAG AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd4 ( XM_006510906.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510969.1 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu00834
Clone ID Related Accession (Same CDS sequence)	NM_001040426.3 , XM_006510906.3 , XM_006510906.4 , XM_006510907.3
Accession Version	XM_006510906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510969.1
CDS	328..3384
Translation	MVSYLTSCLSALSTLLLLLGSQLVCPQPSTEHRKVPQRMAVTEGTPEDSGSGSPGVWGSWGPWSACSRSCSGGVMEQTRPCLPSSYRARGGSRPNGRALSITGHVVSAVRTSVPLHRSQEDQRALAGSNASRQGPAVVRGSRHPQARGREPSERRSRTRGPIGPGKYGYGKAPYILPLQTDTTHTPQRLRRQRPSSRHSRSQEASASKQGYRPPTHQFSHSQPLYQSDSGPRSGLPPSEASIYQLPLTHDQSYPAASSLFHRPELSSHHGARPHGAAQAFPQHLRSTAISCIGAYRQYKLCNTNACPESGRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQATGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	XM_006510906.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510906.4

ORF Insert Sequence:

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd4 ( XM_006510905.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510968.1 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X1, mRNA.

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CloneID	OMu42620
Clone ID Related Accession (Same CDS sequence)	XM_006510905.4 , XM_006510905.3
Accession Version	XM_006510905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCCC TCACAGTTCT GCAGACCAGA TGTCACCAGA TATGTGTGCT TTCCCAAAGT 
TCTGGGTGGA GCATCCTCAG CATCTGGTGG CTCCAGGCCC CCTGTGGCTT TGACTGTATC 
CAGGTCCCAC AACGGATGGC AGTGACCGAG GGAACCCCAG AGGACAGTGG CAGTGGCTCC 
CCAGGTGTGT GGGGCTCCTG GGGCCCCTGG TCTGCCTGTT CTCGCAGCTG CAGTGGTGGT 
GTGATGGAGC AGACACGGCC CTGCCTGCCC AGCTCCTACC GTGCTCGAGG AGGCTCCCGG 
CCCAATGGCC GTGCACTGTC CATCACTGGC CACGTTGTGT CAGCCGTACG GACCTCAGTA 
CCACTGCATC GAAGCCAGGA GGACCAAAGA GCCCTGGCTG GCTCCAATGC TAGCCGCCAA 
GGCCCTGCAG TGGTGCGAGG CAGCCGACAT CCACAGGCCC GGGGCCGTGA ACCCTCAGAG 
AGAAGGAGCA GGACCCGAGG TCCTATTGGC CCTGGCAAGT ATGGCTATGG GAAGGCTCCC 
TACATCTTAC CACTGCAGAC GGATACCACG CATACTCCAC AGAGGCTTCG GAGACAGAGG 
CCTTCATCCA GGCACTCCAG GTCCCAGGAG GCATCTGCTT CTAAGCAGGG CTATCGGCCA 
CCAACCCATC AGTTCTCCCA CAGCCAACCT CTCTACCAGA GTGACAGTGG CCCTCGCTCT 
GGACTGCCAC CTTCTGAGGC TTCTATCTAC CAGCTGCCCT TGACTCACGA CCAAAGCTAT 
CCTGCAGCGT CGAGTCTCTT CCACAGACCG GAACTGAGCA GCCACCATGG TGCTAGGCCC 
CACGGGGCAG CTCAAGCCTT TCCCCAGCAT CTGCGATCAA CAGCAATCTC CTGCATCGGG 
GCCTATCGGC AGTACAAACT GTGCAACACC AATGCGTGTC CAGAAAGTGG TAGAAGTATC 
CGGGAGGTAC AGTGTGCATC CTACAACAAC AAGCCATTCA TGGGCCGGTT TTATGAATGG 
GAACCATTTG CAGAAGTAAA GGGTAATCGC AAGTGTGAGC TGAACTGCCA GGCAACCGGC 
TACCGCTTCT ACGTCCGGCA AGCTGAGAAG GTCATCGATG GCACACCCTG TGATCAGAAT 
GGCACGGCAA TCTGTGTGTC GGGGCAGTGC AAGAGCATTG GCTGTGACGA CTTCCTGGGA 
TCTGACAAAG TCTTGGACAA GTGCGGGGTG TGTGGAGGTG ATAACACGGG CTGCCAGGTG 
GTGTCGGGAG TGTTCAAGCA CGCCCTCACC AGCCTGGGCT ACCACCGGGT TGTAGAGATT 
CCCCAAGGAG CTACGAAAAT TAACATTACA GAGATGCACA AGAGCAACAA CTACTTAGCC 
TTGCGAAGTC GCTCTGGCCG CTCCATCATC AATGGGAACT GGGCAATCGA TCGCCCGGGG 
AAGTACGAGG GCGGAGGGAC AATGTTCACC TACAAACGAC CGAACGAGGT CTCCAGCACT 
GCGGGGGAGT CCTTTCTGGC TGAGGGTCCC ACTAATGAGA TCCTGGATGT CTATATGATA 
CACCAGCAGC CCAACCCAGG AGTCCACTAT GAGTACGTGA TCATGAGGAA TAATGCCATC 
AGCCCACAGG TGCCACCACA CAGGAGACCA GGAGAACCTT TCAATGGGCA GCTGGAAGAG 
GAAGACAGAG GCCAGGAGGA CAGAGAAGAG AGGGAGAAGA ACCAGGAAAA GGAAGACTCA 
CAAGTCGAGG CCCCTGAGGT ATTCACGTCA GAATCCACGC AGACCTTCCC AGTCAGGCAT 
CCGGAGAGAT TCCCTTCCCA CCGACCAGAC AACTTGGTGC CCCCAGCACC ACAGCCCCCA 
AGGCGAAGCC GGGACCACAA CTGGAAGCAG CTGGGGACAA CAGAATGCTC CACCACCTGT 
GGGAAAGGAT CGCAGTACCC TATCTTCCGC TGCGTGCACA GAAACACACA CGAGGAGGTT 
CCCGAGAGCT ACTGTGACTC CAGCATGAAG CCGACCCCAG AGGAGGAACC CTGCAACCTC 
TTCCCCTGCC CAGCCTTCTG GGACATCGGG GAGTGGTCGG AGTGCAGCAA GACCTGTGGT 
CTGGGCATGC AGCACCGCCA GGTCCTGTGT CGCCAGGTGT ATGCCAACCG TAGCCTGACG 
GTGCAACCCT ACCGCTGCCA GCACCTGGAG AAGCCAGAGA CCACCAGCAC CTGCCAGCTC 
AAGATCTGCA GCGAGTGGCA GATTCGGACC GACTGGACCT CGTGCTCAGT GCCCTGTGGC 
GTGGGGCAGC GGACCCGAGA CGTGAAGTGT GTGAGCAACA TCGGGGACAT GGTGCATGAC 
GAGGAGTGCA ACATGAAGCT CCGGCCAAAT GACATCGAGA ACTGTGACAT GGGGCCCTGT 
GCGAAGAGCT GGTTCCTCAC AGAGTGGAGT GAAAGGTGCT CAGCGGAGTG TGGGGCAGGA 
GTGAGGACAC GTTCAGTGGT GTGCATGACC AACCACGTCA GCAGCCTGCC GTTGGAAGGC 
TGTGGGAACA ACAGGCCAGT GGAGGCTACG CCGTGTGACA ATGGGCCCTG CACAGGCAAG 
GTGGAGTGGT TCACTGGCAG CTGGAGTCAG TGTTCCATCG AGTGTGGCAG TGGGACACAG 
CAGAGGGAAG TGATCTGTGT TAGGAAGAAT GCAGACACCT TTGAAGTGCT AGACCCTTAT 
GAGTGTTCAT TCCTGGAGAA GCCCCCAAGC CAGCAGGCCT GCCACCTCAA GCCTTGTGGA 
GCTAAATGGT TTAGCACAGA ATGGAGCATG TGCTCCAAGA GCTGTCAGGG CGGATTCCGG 
GTGCGGGAAG TTCGGTGTCT CTCAGATGAC ATGACCCCAA GTAGCCTCTG CGACCCCCAG 
TTGAAACCAG AAGAGAGAGA GTCTTGTAAC ACTCAGGACT GTGTCCCTGA AGTTGATGAA 
AACTGCAAGG ACAAGTACTA CAACTGCAAC GTGGTGGTCC AGGCACGCCT CTGTGTCTAC 
AACTACTACA AGACTGCCTG CTGTGCCTCT TGCACCCGAG TGGCTAACAG ACACGTGGGC 
TTTCTGGGGA GCCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510968.1
CDS	74..3151
Translation	MNSLTVLQTRCHQICVLSQSSGWSILSIWWLQAPCGFDCIQVPQRMAVTEGTPEDSGSGSPGVWGSWGPWSACSRSCSGGVMEQTRPCLPSSYRARGGSRPNGRALSITGHVVSAVRTSVPLHRSQEDQRALAGSNASRQGPAVVRGSRHPQARGREPSERRSRTRGPIGPGKYGYGKAPYILPLQTDTTHTPQRLRRQRPSSRHSRSQEASASKQGYRPPTHQFSHSQPLYQSDSGPRSGLPPSEASIYQLPLTHDQSYPAASSLFHRPELSSHHGARPHGAAQAFPQHLRSTAISCIGAYRQYKLCNTNACPESGRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQATGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	XM_006510905.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510905.4

ORF Insert Sequence:

ATGAATTCCC TCACAGTTCT GCAGACCAGA TGTCACCAGA TATGTGTGCT TTCCCAAAGT 
TCTGGGTGGA GCATCCTCAG CATCTGGTGG CTCCAGGCCC CCTGTGGCTT TGACTGTATC 
CAGGTCCCAC AACGGATGGC AGTGACCGAG GGAACCCCAG AGGACAGTGG CAGTGGCTCC 
CCAGGTGTGT GGGGCTCCTG GGGCCCCTGG TCTGCCTGTT CTCGCAGCTG CAGTGGTGGT 
GTGATGGAGC AGACACGGCC CTGCCTGCCC AGCTCCTACC GTGCTCGAGG AGGCTCCCGG 
CCCAATGGCC GTGCACTGTC CATCACTGGC CACGTTGTGT CAGCCGTACG GACCTCAGTA 
CCACTGCATC GAAGCCAGGA GGACCAAAGA GCCCTGGCTG GCTCCAATGC TAGCCGCCAA 
GGCCCTGCAG TGGTGCGAGG CAGCCGACAT CCACAGGCCC GGGGCCGTGA ACCCTCAGAG 
AGAAGGAGCA GGACCCGAGG TCCTATTGGC CCTGGCAAGT ATGGCTATGG GAAGGCTCCC 
TACATCTTAC CACTGCAGAC GGATACCACG CATACTCCAC AGAGGCTTCG GAGACAGAGG 
CCTTCATCCA GGCACTCCAG GTCCCAGGAG GCATCTGCTT CTAAGCAGGG CTATCGGCCA 
CCAACCCATC AGTTCTCCCA CAGCCAACCT CTCTACCAGA GTGACAGTGG CCCTCGCTCT 
GGACTGCCAC CTTCTGAGGC TTCTATCTAC CAGCTGCCCT TGACTCACGA CCAAAGCTAT 
CCTGCAGCGT CGAGTCTCTT CCACAGACCG GAACTGAGCA GCCACCATGG TGCTAGGCCC 
CACGGGGCAG CTCAAGCCTT TCCCCAGCAT CTGCGATCAA CAGCAATCTC CTGCATCGGG 
GCCTATCGGC AGTACAAACT GTGCAACACC AATGCGTGTC CAGAAAGTGG TAGAAGTATC 
CGGGAGGTAC AGTGTGCATC CTACAACAAC AAGCCATTCA TGGGCCGGTT TTATGAATGG 
GAACCATTTG CAGAAGTAAA GGGTAATCGC AAGTGTGAGC TGAACTGCCA GGCAACCGGC 
TACCGCTTCT ACGTCCGGCA AGCTGAGAAG GTCATCGATG GCACACCCTG TGATCAGAAT 
GGCACGGCAA TCTGTGTGTC GGGGCAGTGC AAGAGCATTG GCTGTGACGA CTTCCTGGGA 
TCTGACAAAG TCTTGGACAA GTGCGGGGTG TGTGGAGGTG ATAACACGGG CTGCCAGGTG 
GTGTCGGGAG TGTTCAAGCA CGCCCTCACC AGCCTGGGCT ACCACCGGGT TGTAGAGATT 
CCCCAAGGAG CTACGAAAAT TAACATTACA GAGATGCACA AGAGCAACAA CTACTTAGCC 
TTGCGAAGTC GCTCTGGCCG CTCCATCATC AATGGGAACT GGGCAATCGA TCGCCCGGGG 
AAGTACGAGG GCGGAGGGAC AATGTTCACC TACAAACGAC CGAACGAGGT CTCCAGCACT 
GCGGGGGAGT CCTTTCTGGC TGAGGGTCCC ACTAATGAGA TCCTGGATGT CTATATGATA 
CACCAGCAGC CCAACCCAGG AGTCCACTAT GAGTACGTGA TCATGAGGAA TAATGCCATC 
AGCCCACAGG TGCCACCACA CAGGAGACCA GGAGAACCTT TCAATGGGCA GCTGGAAGAG 
GAAGACAGAG GCCAGGAGGA CAGAGAAGAG AGGGAGAAGA ACCAGGAAAA GGAAGACTCA 
CAAGTCGAGG CCCCTGAGGT ATTCACGTCA GAATCCACGC AGACCTTCCC AGTCAGGCAT 
CCGGAGAGAT TCCCTTCCCA CCGACCAGAC AACTTGGTGC CCCCAGCACC ACAGCCCCCA 
AGGCGAAGCC GGGACCACAA CTGGAAGCAG CTGGGGACAA CAGAATGCTC CACCACCTGT 
GGGAAAGGAT CGCAGTACCC TATCTTCCGC TGCGTGCACA GAAACACACA CGAGGAGGTT 
CCCGAGAGCT ACTGTGACTC CAGCATGAAG CCGACCCCAG AGGAGGAACC CTGCAACCTC 
TTCCCCTGCC CAGCCTTCTG GGACATCGGG GAGTGGTCGG AGTGCAGCAA GACCTGTGGT 
CTGGGCATGC AGCACCGCCA GGTCCTGTGT CGCCAGGTGT ATGCCAACCG TAGCCTGACG 
GTGCAACCCT ACCGCTGCCA GCACCTGGAG AAGCCAGAGA CCACCAGCAC CTGCCAGCTC 
AAGATCTGCA GCGAGTGGCA GATTCGGACC GACTGGACCT CGTGCTCAGT GCCCTGTGGC 
GTGGGGCAGC GGACCCGAGA CGTGAAGTGT GTGAGCAACA TCGGGGACAT GGTGCATGAC 
GAGGAGTGCA ACATGAAGCT CCGGCCAAAT GACATCGAGA ACTGTGACAT GGGGCCCTGT 
GCGAAGAGCT GGTTCCTCAC AGAGTGGAGT GAAAGGTGCT CAGCGGAGTG TGGGGCAGGA 
GTGAGGACAC GTTCAGTGGT GTGCATGACC AACCACGTCA GCAGCCTGCC GTTGGAAGGC 
TGTGGGAACA ACAGGCCAGT GGAGGCTACG CCGTGTGACA ATGGGCCCTG CACAGGCAAG 
GTGGAGTGGT TCACTGGCAG CTGGAGTCAG TGTTCCATCG AGTGTGGCAG TGGGACACAG 
CAGAGGGAAG TGATCTGTGT TAGGAAGAAT GCAGACACCT TTGAAGTGCT AGACCCTTAT 
GAGTGTTCAT TCCTGGAGAA GCCCCCAAGC CAGCAGGCCT GCCACCTCAA GCCTTGTGGA 
GCTAAATGGT TTAGCACAGA ATGGAGCATG TGCTCCAAGA GCTGTCAGGG CGGATTCCGG 
GTGCGGGAAG TTCGGTGTCT CTCAGATGAC ATGACCCCAA GTAGCCTCTG CGACCCCCAG 
TTGAAACCAG AAGAGAGAGA GTCTTGTAAC ACTCAGGACT GTGTCCCTGA AGTTGATGAA 
AACTGCAAGG ACAAGTACTA CAACTGCAAC GTGGTGGTCC AGGCACGCCT CTGTGTCTAC 
AACTACTACA AGACTGCCTG CTGTGCCTCT TGCACCCGAG TGGCTAACAG ACACGTGGGC 
TTTCTGGGGA GCCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd4 ( NM_001040426.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001035516.2 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 1, mRNA.

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CloneID	OMu00834
Clone ID Related Accession (Same CDS sequence)	NM_001040426.3 , XM_006510906.3 , XM_006510906.4 , XM_006510907.3
Accession Version	NM_001040426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138976.1 and AC131749.2. On Jan 6, 2010 this sequence version replaced NM_001040426.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK138976.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035516.2
CDS	275..3331
Translation	MVSYLTSCLSALSTLLLLLGSQLVCPQPSTEHRKVPQRMAVTEGTPEDSGSGSPGVWGSWGPWSACSRSCSGGVMEQTRPCLPSSYRARGGSRPNGRALSITGHVVSAVRTSVPLHRSQEDQRALAGSNASRQGPAVVRGSRHPQARGREPSERRSRTRGPIGPGKYGYGKAPYILPLQTDTTHTPQRLRRQRPSSRHSRSQEASASKQGYRPPTHQFSHSQPLYQSDSGPRSGLPPSEASIYQLPLTHDQSYPAASSLFHRPELSSHHGARPHGAAQAFPQHLRSTAISCIGAYRQYKLCNTNACPESGRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQATGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	NM_001040426.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040426.3

ORF Insert Sequence:

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd4 ( XM_006510906.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510969.1 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu00834
Clone ID Related Accession (Same CDS sequence)	NM_001040426.3 , XM_006510906.3 , XM_006510906.4 , XM_006510907.3
Accession Version	XM_006510906.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510969.1
CDS	389..3445
Translation	MVSYLTSCLSALSTLLLLLGSQLVCPQPSTEHRKVPQRMAVTEGTPEDSGSGSPGVWGSWGPWSACSRSCSGGVMEQTRPCLPSSYRARGGSRPNGRALSITGHVVSAVRTSVPLHRSQEDQRALAGSNASRQGPAVVRGSRHPQARGREPSERRSRTRGPIGPGKYGYGKAPYILPLQTDTTHTPQRLRRQRPSSRHSRSQEASASKQGYRPPTHQFSHSQPLYQSDSGPRSGLPPSEASIYQLPLTHDQSYPAASSLFHRPELSSHHGARPHGAAQAFPQHLRSTAISCIGAYRQYKLCNTNACPESGRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQATGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	XM_006510906.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510906.3

ORF Insert Sequence:

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd4 ( XM_006510907.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510970.1 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu00834
Clone ID Related Accession (Same CDS sequence)	NM_001040426.3 , XM_006510906.3 , XM_006510906.4 , XM_006510907.3
Accession Version	XM_006510907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510970.1
CDS	156..3212
Translation	MVSYLTSCLSALSTLLLLLGSQLVCPQPSTEHRKVPQRMAVTEGTPEDSGSGSPGVWGSWGPWSACSRSCSGGVMEQTRPCLPSSYRARGGSRPNGRALSITGHVVSAVRTSVPLHRSQEDQRALAGSNASRQGPAVVRGSRHPQARGREPSERRSRTRGPIGPGKYGYGKAPYILPLQTDTTHTPQRLRRQRPSSRHSRSQEASASKQGYRPPTHQFSHSQPLYQSDSGPRSGLPPSEASIYQLPLTHDQSYPAASSLFHRPELSSHHGARPHGAAQAFPQHLRSTAISCIGAYRQYKLCNTNACPESGRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQATGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	XM_006510907.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510907.3

ORF Insert Sequence:

ATGGTTTCCT ATCTCACGAG CTGTCTCAGT GCCCTGAGCA CCCTCCTGCT GCTGCTTGGT 
TCTCAGCTTG TCTGCCCGCA GCCTTCCACT GAACACAGAA AGGTCCCACA ACGGATGGCA 
GTGACCGAGG GAACCCCAGA GGACAGTGGC AGTGGCTCCC CAGGTGTGTG GGGCTCCTGG 
GGCCCCTGGT CTGCCTGTTC TCGCAGCTGC AGTGGTGGTG TGATGGAGCA GACACGGCCC 
TGCCTGCCCA GCTCCTACCG TGCTCGAGGA GGCTCCCGGC CCAATGGCCG TGCACTGTCC 
ATCACTGGCC ACGTTGTGTC AGCCGTACGG ACCTCAGTAC CACTGCATCG AAGCCAGGAG 
GACCAAAGAG CCCTGGCTGG CTCCAATGCT AGCCGCCAAG GCCCTGCAGT GGTGCGAGGC 
AGCCGACATC CACAGGCCCG GGGCCGTGAA CCCTCAGAGA GAAGGAGCAG GACCCGAGGT 
CCTATTGGCC CTGGCAAGTA TGGCTATGGG AAGGCTCCCT ACATCTTACC ACTGCAGACG 
GATACCACGC ATACTCCACA GAGGCTTCGG AGACAGAGGC CTTCATCCAG GCACTCCAGG 
TCCCAGGAGG CATCTGCTTC TAAGCAGGGC TATCGGCCAC CAACCCATCA GTTCTCCCAC 
AGCCAACCTC TCTACCAGAG TGACAGTGGC CCTCGCTCTG GACTGCCACC TTCTGAGGCT 
TCTATCTACC AGCTGCCCTT GACTCACGAC CAAAGCTATC CTGCAGCGTC GAGTCTCTTC 
CACAGACCGG AACTGAGCAG CCACCATGGT GCTAGGCCCC ACGGGGCAGC TCAAGCCTTT 
CCCCAGCATC TGCGATCAAC AGCAATCTCC TGCATCGGGG CCTATCGGCA GTACAAACTG 
TGCAACACCA ATGCGTGTCC AGAAAGTGGT AGAAGTATCC GGGAGGTACA GTGTGCATCC 
TACAACAACA AGCCATTCAT GGGCCGGTTT TATGAATGGG AACCATTTGC AGAAGTAAAG 
GGTAATCGCA AGTGTGAGCT GAACTGCCAG GCAACCGGCT ACCGCTTCTA CGTCCGGCAA 
GCTGAGAAGG TCATCGATGG CACACCCTGT GATCAGAATG GCACGGCAAT CTGTGTGTCG 
GGGCAGTGCA AGAGCATTGG CTGTGACGAC TTCCTGGGAT CTGACAAAGT CTTGGACAAG 
TGCGGGGTGT GTGGAGGTGA TAACACGGGC TGCCAGGTGG TGTCGGGAGT GTTCAAGCAC 
GCCCTCACCA GCCTGGGCTA CCACCGGGTT GTAGAGATTC CCCAAGGAGC TACGAAAATT 
AACATTACAG AGATGCACAA GAGCAACAAC TACTTAGCCT TGCGAAGTCG CTCTGGCCGC 
TCCATCATCA ATGGGAACTG GGCAATCGAT CGCCCGGGGA AGTACGAGGG CGGAGGGACA 
ATGTTCACCT ACAAACGACC GAACGAGGTC TCCAGCACTG CGGGGGAGTC CTTTCTGGCT 
GAGGGTCCCA CTAATGAGAT CCTGGATGTC TATATGATAC ACCAGCAGCC CAACCCAGGA 
GTCCACTATG AGTACGTGAT CATGAGGAAT AATGCCATCA GCCCACAGGT GCCACCACAC 
AGGAGACCAG GAGAACCTTT CAATGGGCAG CTGGAAGAGG AAGACAGAGG CCAGGAGGAC 
AGAGAAGAGA GGGAGAAGAA CCAGGAAAAG GAAGACTCAC AAGTCGAGGC CCCTGAGGTA 
TTCACGTCAG AATCCACGCA GACCTTCCCA GTCAGGCATC CGGAGAGATT CCCTTCCCAC 
CGACCAGACA ACTTGGTGCC CCCAGCACCA CAGCCCCCAA GGCGAAGCCG GGACCACAAC 
TGGAAGCAGC TGGGGACAAC AGAATGCTCC ACCACCTGTG GGAAAGGATC GCAGTACCCT 
ATCTTCCGCT GCGTGCACAG AAACACACAC GAGGAGGTTC CCGAGAGCTA CTGTGACTCC 
AGCATGAAGC CGACCCCAGA GGAGGAACCC TGCAACCTCT TCCCCTGCCC AGCCTTCTGG 
GACATCGGGG AGTGGTCGGA GTGCAGCAAG ACCTGTGGTC TGGGCATGCA GCACCGCCAG 
GTCCTGTGTC GCCAGGTGTA TGCCAACCGT AGCCTGACGG TGCAACCCTA CCGCTGCCAG 
CACCTGGAGA AGCCAGAGAC CACCAGCACC TGCCAGCTCA AGATCTGCAG CGAGTGGCAG 
ATTCGGACCG ACTGGACCTC GTGCTCAGTG CCCTGTGGCG TGGGGCAGCG GACCCGAGAC 
GTGAAGTGTG TGAGCAACAT CGGGGACATG GTGCATGACG AGGAGTGCAA CATGAAGCTC 
CGGCCAAATG ACATCGAGAA CTGTGACATG GGGCCCTGTG CGAAGAGCTG GTTCCTCACA 
GAGTGGAGTG AAAGGTGCTC AGCGGAGTGT GGGGCAGGAG TGAGGACACG TTCAGTGGTG 
TGCATGACCA ACCACGTCAG CAGCCTGCCG TTGGAAGGCT GTGGGAACAA CAGGCCAGTG 
GAGGCTACGC CGTGTGACAA TGGGCCCTGC ACAGGCAAGG TGGAGTGGTT CACTGGCAGC 
TGGAGTCAGT GTTCCATCGA GTGTGGCAGT GGGACACAGC AGAGGGAAGT GATCTGTGTT 
AGGAAGAATG CAGACACCTT TGAAGTGCTA GACCCTTATG AGTGTTCATT CCTGGAGAAG 
CCCCCAAGCC AGCAGGCCTG CCACCTCAAG CCTTGTGGAG CTAAATGGTT TAGCACAGAA 
TGGAGCATGT GCTCCAAGAG CTGTCAGGGC GGATTCCGGG TGCGGGAAGT TCGGTGTCTC 
TCAGATGACA TGACCCCAAG TAGCCTCTGC GACCCCCAGT TGAAACCAGA AGAGAGAGAG 
TCTTGTAACA CTCAGGACTG TGTCCCTGAA GTTGATGAAA ACTGCAAGGA CAAGTACTAC 
AACTGCAACG TGGTGGTCCA GGCACGCCTC TGTGTCTACA ACTACTACAA GACTGCCTGC 
TGTGCCTCTT GCACCCGAGT GGCTAACAGA CACGTGGGCT TTCTGGGGAG CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Thsd4 ( XM_006510905.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510968.1 Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X1, mRNA.

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CloneID	OMu42620
Clone ID Related Accession (Same CDS sequence)	XM_006510905.4 , XM_006510905.3
Accession Version	XM_006510905.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	thrombospondin type-1 domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCCC TCACAGTTCT GCAGACCAGA TGTCACCAGA TATGTGTGCT TTCCCAAAGT 
TCTGGGTGGA GCATCCTCAG CATCTGGTGG CTCCAGGCCC CCTGTGGCTT TGACTGTATC 
CAGGTCCCAC AACGGATGGC AGTGACCGAG GGAACCCCAG AGGACAGTGG CAGTGGCTCC 
CCAGGTGTGT GGGGCTCCTG GGGCCCCTGG TCTGCCTGTT CTCGCAGCTG CAGTGGTGGT 
GTGATGGAGC AGACACGGCC CTGCCTGCCC AGCTCCTACC GTGCTCGAGG AGGCTCCCGG 
CCCAATGGCC GTGCACTGTC CATCACTGGC CACGTTGTGT CAGCCGTACG GACCTCAGTA 
CCACTGCATC GAAGCCAGGA GGACCAAAGA GCCCTGGCTG GCTCCAATGC TAGCCGCCAA 
GGCCCTGCAG TGGTGCGAGG CAGCCGACAT CCACAGGCCC GGGGCCGTGA ACCCTCAGAG 
AGAAGGAGCA GGACCCGAGG TCCTATTGGC CCTGGCAAGT ATGGCTATGG GAAGGCTCCC 
TACATCTTAC CACTGCAGAC GGATACCACG CATACTCCAC AGAGGCTTCG GAGACAGAGG 
CCTTCATCCA GGCACTCCAG GTCCCAGGAG GCATCTGCTT CTAAGCAGGG CTATCGGCCA 
CCAACCCATC AGTTCTCCCA CAGCCAACCT CTCTACCAGA GTGACAGTGG CCCTCGCTCT 
GGACTGCCAC CTTCTGAGGC TTCTATCTAC CAGCTGCCCT TGACTCACGA CCAAAGCTAT 
CCTGCAGCGT CGAGTCTCTT CCACAGACCG GAACTGAGCA GCCACCATGG TGCTAGGCCC 
CACGGGGCAG CTCAAGCCTT TCCCCAGCAT CTGCGATCAA CAGCAATCTC CTGCATCGGG 
GCCTATCGGC AGTACAAACT GTGCAACACC AATGCGTGTC CAGAAAGTGG TAGAAGTATC 
CGGGAGGTAC AGTGTGCATC CTACAACAAC AAGCCATTCA TGGGCCGGTT TTATGAATGG 
GAACCATTTG CAGAAGTAAA GGGTAATCGC AAGTGTGAGC TGAACTGCCA GGCAACCGGC 
TACCGCTTCT ACGTCCGGCA AGCTGAGAAG GTCATCGATG GCACACCCTG TGATCAGAAT 
GGCACGGCAA TCTGTGTGTC GGGGCAGTGC AAGAGCATTG GCTGTGACGA CTTCCTGGGA 
TCTGACAAAG TCTTGGACAA GTGCGGGGTG TGTGGAGGTG ATAACACGGG CTGCCAGGTG 
GTGTCGGGAG TGTTCAAGCA CGCCCTCACC AGCCTGGGCT ACCACCGGGT TGTAGAGATT 
CCCCAAGGAG CTACGAAAAT TAACATTACA GAGATGCACA AGAGCAACAA CTACTTAGCC 
TTGCGAAGTC GCTCTGGCCG CTCCATCATC AATGGGAACT GGGCAATCGA TCGCCCGGGG 
AAGTACGAGG GCGGAGGGAC AATGTTCACC TACAAACGAC CGAACGAGGT CTCCAGCACT 
GCGGGGGAGT CCTTTCTGGC TGAGGGTCCC ACTAATGAGA TCCTGGATGT CTATATGATA 
CACCAGCAGC CCAACCCAGG AGTCCACTAT GAGTACGTGA TCATGAGGAA TAATGCCATC 
AGCCCACAGG TGCCACCACA CAGGAGACCA GGAGAACCTT TCAATGGGCA GCTGGAAGAG 
GAAGACAGAG GCCAGGAGGA CAGAGAAGAG AGGGAGAAGA ACCAGGAAAA GGAAGACTCA 
CAAGTCGAGG CCCCTGAGGT ATTCACGTCA GAATCCACGC AGACCTTCCC AGTCAGGCAT 
CCGGAGAGAT TCCCTTCCCA CCGACCAGAC AACTTGGTGC CCCCAGCACC ACAGCCCCCA 
AGGCGAAGCC GGGACCACAA CTGGAAGCAG CTGGGGACAA CAGAATGCTC CACCACCTGT 
GGGAAAGGAT CGCAGTACCC TATCTTCCGC TGCGTGCACA GAAACACACA CGAGGAGGTT 
CCCGAGAGCT ACTGTGACTC CAGCATGAAG CCGACCCCAG AGGAGGAACC CTGCAACCTC 
TTCCCCTGCC CAGCCTTCTG GGACATCGGG GAGTGGTCGG AGTGCAGCAA GACCTGTGGT 
CTGGGCATGC AGCACCGCCA GGTCCTGTGT CGCCAGGTGT ATGCCAACCG TAGCCTGACG 
GTGCAACCCT ACCGCTGCCA GCACCTGGAG AAGCCAGAGA CCACCAGCAC CTGCCAGCTC 
AAGATCTGCA GCGAGTGGCA GATTCGGACC GACTGGACCT CGTGCTCAGT GCCCTGTGGC 
GTGGGGCAGC GGACCCGAGA CGTGAAGTGT GTGAGCAACA TCGGGGACAT GGTGCATGAC 
GAGGAGTGCA ACATGAAGCT CCGGCCAAAT GACATCGAGA ACTGTGACAT GGGGCCCTGT 
GCGAAGAGCT GGTTCCTCAC AGAGTGGAGT GAAAGGTGCT CAGCGGAGTG TGGGGCAGGA 
GTGAGGACAC GTTCAGTGGT GTGCATGACC AACCACGTCA GCAGCCTGCC GTTGGAAGGC 
TGTGGGAACA ACAGGCCAGT GGAGGCTACG CCGTGTGACA ATGGGCCCTG CACAGGCAAG 
GTGGAGTGGT TCACTGGCAG CTGGAGTCAG TGTTCCATCG AGTGTGGCAG TGGGACACAG 
CAGAGGGAAG TGATCTGTGT TAGGAAGAAT GCAGACACCT TTGAAGTGCT AGACCCTTAT 
GAGTGTTCAT TCCTGGAGAA GCCCCCAAGC CAGCAGGCCT GCCACCTCAA GCCTTGTGGA 
GCTAAATGGT TTAGCACAGA ATGGAGCATG TGCTCCAAGA GCTGTCAGGG CGGATTCCGG 
GTGCGGGAAG TTCGGTGTCT CTCAGATGAC ATGACCCCAA GTAGCCTCTG CGACCCCCAG 
TTGAAACCAG AAGAGAGAGA GTCTTGTAAC ACTCAGGACT GTGTCCCTGA AGTTGATGAA 
AACTGCAAGG ACAAGTACTA CAACTGCAAC GTGGTGGTCC AGGCACGCCT CTGTGTCTAC 
AACTACTACA AGACTGCCTG CTGTGCCTCT TGCACCCGAG TGGCTAACAG ACACGTGGGC 
TTTCTGGGGA GCCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510968.1
CDS	75..3152
Translation	MNSLTVLQTRCHQICVLSQSSGWSILSIWWLQAPCGFDCIQVPQRMAVTEGTPEDSGSGSPGVWGSWGPWSACSRSCSGGVMEQTRPCLPSSYRARGGSRPNGRALSITGHVVSAVRTSVPLHRSQEDQRALAGSNASRQGPAVVRGSRHPQARGREPSERRSRTRGPIGPGKYGYGKAPYILPLQTDTTHTPQRLRRQRPSSRHSRSQEASASKQGYRPPTHQFSHSQPLYQSDSGPRSGLPPSEASIYQLPLTHDQSYPAASSLFHRPELSSHHGARPHGAAQAFPQHLRSTAISCIGAYRQYKLCNTNACPESGRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQATGYRFYVRQAEKVIDGTPCDQNGTAICVSGQCKSIGCDDFLGSDKVLDKCGVCGGDNTGCQVVSGVFKHALTSLGYHRVVEIPQGATKINITEMHKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYKRPNEVSSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMRNNAISPQVPPHRRPGEPFNGQLEEEDRGQEDREEREKNQEKEDSQVEAPEVFTSESTQTFPVRHPERFPSHRPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRNTHEEVPESYCDSSMKPTPEEEPCNLFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQHLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDMVHDEECNMKLRPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPVEATPCDNGPCTGKVEWFTGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPYECSFLEKPPSQQACHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTPSSLCDPQLKPEERESCNTQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRHVGFLGSR

Target ORF information:

RefSeq Version	XM_006510905.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thrombospondin, type I, domain containing 4 (Thsd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510905.3

ORF Insert Sequence:

ATGAATTCCC TCACAGTTCT GCAGACCAGA TGTCACCAGA TATGTGTGCT TTCCCAAAGT 
TCTGGGTGGA GCATCCTCAG CATCTGGTGG CTCCAGGCCC CCTGTGGCTT TGACTGTATC 
CAGGTCCCAC AACGGATGGC AGTGACCGAG GGAACCCCAG AGGACAGTGG CAGTGGCTCC 
CCAGGTGTGT GGGGCTCCTG GGGCCCCTGG TCTGCCTGTT CTCGCAGCTG CAGTGGTGGT 
GTGATGGAGC AGACACGGCC CTGCCTGCCC AGCTCCTACC GTGCTCGAGG AGGCTCCCGG 
CCCAATGGCC GTGCACTGTC CATCACTGGC CACGTTGTGT CAGCCGTACG GACCTCAGTA 
CCACTGCATC GAAGCCAGGA GGACCAAAGA GCCCTGGCTG GCTCCAATGC TAGCCGCCAA 
GGCCCTGCAG TGGTGCGAGG CAGCCGACAT CCACAGGCCC GGGGCCGTGA ACCCTCAGAG 
AGAAGGAGCA GGACCCGAGG TCCTATTGGC CCTGGCAAGT ATGGCTATGG GAAGGCTCCC 
TACATCTTAC CACTGCAGAC GGATACCACG CATACTCCAC AGAGGCTTCG GAGACAGAGG 
CCTTCATCCA GGCACTCCAG GTCCCAGGAG GCATCTGCTT CTAAGCAGGG CTATCGGCCA 
CCAACCCATC AGTTCTCCCA CAGCCAACCT CTCTACCAGA GTGACAGTGG CCCTCGCTCT 
GGACTGCCAC CTTCTGAGGC TTCTATCTAC CAGCTGCCCT TGACTCACGA CCAAAGCTAT 
CCTGCAGCGT CGAGTCTCTT CCACAGACCG GAACTGAGCA GCCACCATGG TGCTAGGCCC 
CACGGGGCAG CTCAAGCCTT TCCCCAGCAT CTGCGATCAA CAGCAATCTC CTGCATCGGG 
GCCTATCGGC AGTACAAACT GTGCAACACC AATGCGTGTC CAGAAAGTGG TAGAAGTATC 
CGGGAGGTAC AGTGTGCATC CTACAACAAC AAGCCATTCA TGGGCCGGTT TTATGAATGG 
GAACCATTTG CAGAAGTAAA GGGTAATCGC AAGTGTGAGC TGAACTGCCA GGCAACCGGC 
TACCGCTTCT ACGTCCGGCA AGCTGAGAAG GTCATCGATG GCACACCCTG TGATCAGAAT 
GGCACGGCAA TCTGTGTGTC GGGGCAGTGC AAGAGCATTG GCTGTGACGA CTTCCTGGGA 
TCTGACAAAG TCTTGGACAA GTGCGGGGTG TGTGGAGGTG ATAACACGGG CTGCCAGGTG 
GTGTCGGGAG TGTTCAAGCA CGCCCTCACC AGCCTGGGCT ACCACCGGGT TGTAGAGATT 
CCCCAAGGAG CTACGAAAAT TAACATTACA GAGATGCACA AGAGCAACAA CTACTTAGCC 
TTGCGAAGTC GCTCTGGCCG CTCCATCATC AATGGGAACT GGGCAATCGA TCGCCCGGGG 
AAGTACGAGG GCGGAGGGAC AATGTTCACC TACAAACGAC CGAACGAGGT CTCCAGCACT 
GCGGGGGAGT CCTTTCTGGC TGAGGGTCCC ACTAATGAGA TCCTGGATGT CTATATGATA 
CACCAGCAGC CCAACCCAGG AGTCCACTAT GAGTACGTGA TCATGAGGAA TAATGCCATC 
AGCCCACAGG TGCCACCACA CAGGAGACCA GGAGAACCTT TCAATGGGCA GCTGGAAGAG 
GAAGACAGAG GCCAGGAGGA CAGAGAAGAG AGGGAGAAGA ACCAGGAAAA GGAAGACTCA 
CAAGTCGAGG CCCCTGAGGT ATTCACGTCA GAATCCACGC AGACCTTCCC AGTCAGGCAT 
CCGGAGAGAT TCCCTTCCCA CCGACCAGAC AACTTGGTGC CCCCAGCACC ACAGCCCCCA 
AGGCGAAGCC GGGACCACAA CTGGAAGCAG CTGGGGACAA CAGAATGCTC CACCACCTGT 
GGGAAAGGAT CGCAGTACCC TATCTTCCGC TGCGTGCACA GAAACACACA CGAGGAGGTT 
CCCGAGAGCT ACTGTGACTC CAGCATGAAG CCGACCCCAG AGGAGGAACC CTGCAACCTC 
TTCCCCTGCC CAGCCTTCTG GGACATCGGG GAGTGGTCGG AGTGCAGCAA GACCTGTGGT 
CTGGGCATGC AGCACCGCCA GGTCCTGTGT CGCCAGGTGT ATGCCAACCG TAGCCTGACG 
GTGCAACCCT ACCGCTGCCA GCACCTGGAG AAGCCAGAGA CCACCAGCAC CTGCCAGCTC 
AAGATCTGCA GCGAGTGGCA GATTCGGACC GACTGGACCT CGTGCTCAGT GCCCTGTGGC 
GTGGGGCAGC GGACCCGAGA CGTGAAGTGT GTGAGCAACA TCGGGGACAT GGTGCATGAC 
GAGGAGTGCA ACATGAAGCT CCGGCCAAAT GACATCGAGA ACTGTGACAT GGGGCCCTGT 
GCGAAGAGCT GGTTCCTCAC AGAGTGGAGT GAAAGGTGCT CAGCGGAGTG TGGGGCAGGA 
GTGAGGACAC GTTCAGTGGT GTGCATGACC AACCACGTCA GCAGCCTGCC GTTGGAAGGC 
TGTGGGAACA ACAGGCCAGT GGAGGCTACG CCGTGTGACA ATGGGCCCTG CACAGGCAAG 
GTGGAGTGGT TCACTGGCAG CTGGAGTCAG TGTTCCATCG AGTGTGGCAG TGGGACACAG 
CAGAGGGAAG TGATCTGTGT TAGGAAGAAT GCAGACACCT TTGAAGTGCT AGACCCTTAT 
GAGTGTTCAT TCCTGGAGAA GCCCCCAAGC CAGCAGGCCT GCCACCTCAA GCCTTGTGGA 
GCTAAATGGT TTAGCACAGA ATGGAGCATG TGCTCCAAGA GCTGTCAGGG CGGATTCCGG 
GTGCGGGAAG TTCGGTGTCT CTCAGATGAC ATGACCCCAA GTAGCCTCTG CGACCCCCAG 
TTGAAACCAG AAGAGAGAGA GTCTTGTAAC ACTCAGGACT GTGTCCCTGA AGTTGATGAA 
AACTGCAAGG ACAAGTACTA CAACTGCAAC GTGGTGGTCC AGGCACGCCT CTGTGTCTAC 
AACTACTACA AGACTGCCTG CTGTGCCTCT TGCACCCGAG TGGCTAACAG ACACGTGGGC 
TTTCTGGGGA GCCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac physiologic and genetic predictors of hyperoxia-induced acute lung injury in mice.
American journal of respiratory cell and molecular biology46(4)470-8(2012 Apr)
Howden R,Cho HY,Miller-DeGraff L,Walker C,Clark JA,Myers PH,Rouse DC,Kleeberger SR

ADAMTSL6? protein rescues fibrillin-1 microfibril disorder in a Marfan syndrome mouse model through the promotion of fibrillin-1 assembly.
The Journal of biological chemistry286(44)38602-13(2011 Nov)
Saito M,Kurokawa M,Oda M,Oshima M,Tsutsui K,Kosaka K,Nakao K,Ogawa M,Manabe R,Suda N,Ganjargal G,Hada Y,Noguchi T,Teranaka T,Sekiguchi K,Yoneda T,Tsuji T

Altered gene expression and spongiotrophoblast differentiation in placenta from a mouse model of diabetes in pregnancy.
Diabetologia54(7)1909-20(2011 Jul)
Salbaum JM,Kruger C,Zhang X,Delahaye NA,Pavlinkova G,Burk DH,Kappen C

O-fucosylation of thrombospondin type 1 repeats restricts epithelial to mesenchymal transition (EMT) and maintains epiblast pluripotency during mouse gastrulation.
Developmental biology346(1)25-38(2010 Oct)
Du J,Takeuchi H,Leonhard-Melief C,Shroyer KR,Dlugosz M,Haltiwanger RS,Holdener BC

A genomic atlas of mouse hypothalamic development.
Nature neuroscience13(6)767-75(2010 Jun)
Shimogori T,Lee DA,Miranda-Angulo A,Yang Y,Wang H,Jiang L,Yoshida AC,Kataoka A,Mashiko H,Avetisyan M,Qi L,Qian J,Blackshaw S

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Thsd4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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