Sptan1 cDNA ORF clone, Mus musculus(house mouse)

The following Sptan1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptan1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu84938 NM_001369325.1
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu29394 XM_006497813.4
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu29396 XM_006497815.4
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu29397 XM_006497816.4
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu19460 NM_001177667.1
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu19650 NM_001177668.1
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu68779 NM_001309460.1
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu29390 XM_006497809.2
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu29391 XM_006497810.2
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu29393 XM_006497812.2
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu29395 XM_006497814.2
Latest version!
Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OMu29394 XM_006497813.3 Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu29397 XM_006497816.3 Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu29396 XM_006497815.3 Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu84938
    Clone ID Related Accession (Same CDS sequence) NM_001369325.1
    Accession Version NM_001369325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL928926.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAGACAC GCATAACTAA GGAGGCCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC
    GCCAATGCTG ACCGAATCCG TGGAGTTATC GACATGGGCA ACTCCCTCAT TGAGCGTGGG
    GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG
    CAGTTCCTGG TGCAGAAATC AGCTGAGAAG AGCCAGAAGC TAAAAGAGGC AAATAAGCAG
    CAGAACTTCA ACACTGGAAT CAAAGACTTT GACTTCTGGC TCTCTGAGGT GGAGGCTCTC
    CTGGCATCGG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT GAAAAAGCAT
    CAGCTGCTAG AGGCAGACAT CTCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG
    GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC
    ACCATCAATG GACGCTTCCA GAAGATCAAG AGCATGGCCA CCTCCCGAAG AGCGAAACTG
    AGCGAGTCCC ACCGCCTGCA CCAGTTCTTC CGGGACATGG ACGACGAGGA GTCCTGGATC
    AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAG
    AATCTGAGGA AGAAACACAA GCGGCTAGAA GCTGAACTGG CTGCACATGA ACCCGCCATT
    CAGGGTGTCC TGGACACTGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG
    ATCCAGCAAC GGCTTGCACA GTTTGTGGAG CACTGGAAGG AACTAAAGCA GCTGGCGGCT
    GCGCGGGGCC AGCGGCTAGA GGAGTCCTTG GAGTATCAGC AGTTTGTAGC CAACGTGGAA
    GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACTCTGGTGG CCAGCGAAGA CTATGGAGAC
    ACTCTTGCTG CTATCCAGGG CTTACTGAAG AAACACGAAG CTTTTGAGAC AGACTTCACT
    GTCCACAAGG ATCGGGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGAAA GGTGTCTGAT
    CTGGAGAAAG CAGCAGCTCA GAGGAAAGCA AAGCTGGATG AGAACTCAGC CTTCCTTCAG
    TTCAACTGGA AGGCTGACGT GGTAGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA
    ACAGATGACT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACAAA ACAGGAGACA
    TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGTATTG CCAATATCAC TGCCCTCAAA
    GACCAGCTGC TAGCTGCCAA GCACATTCAG TCTAAGGCCA TCGAGGCCCG ACATGCCTCC
    CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGGAAGAA GAAGTTGCTG
    GAGGCCCAGA GTCATTTCCG TAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA
    TCGGCTTTCA ATAGCTGGTT TGAGAATGCA GAGGAAGACC TCACAGACCC AGTGCGCTGC
    AACTCTCTGG AAGAAATCAA AGCCCTTCGA GAGGCTCACG ATGCCTTCCG CTCATCCCTC
    AGCTCTGCAC AGGCTGACTT CAACCAGCTA GCCGAGCTGG ACCGCCAGAT CAAGAGTTTC
    CGGGTGGCCT CCAACCCCTA TACCTGGTTC ACGATGGAGG CCCTGGAAGA GACCTGGAGA
    AACCTCCAGA AGATCATTAA GGAACGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG
    GAGGAGAATG ACAAGCTGCG CCAAGAATTT GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGGTCCTGCA TGGTGGAAGA GTCGGGAACT
    CTGGAATCTC AGCTTGAAGC TACCAAACGC AAGCACCAGG AGATTCGAGC CATGAGAAGT
    CAGCTGAAGA AGATCGAGGA CCTGGGGGCT GCCATGGAGG AAGCCCTCAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC TGTGGGCCTG GCCCAGCAGT GGGACCAGTT AGACCAGCTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGAGTG
    ACTGAGGAGG CCCTCAAGGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCATCAGGA GTTCAAATCC TGTCTTCGCT CCCTGGGCTA TGATCTGCCA
    ATGGTTGAGG AAGGAGAGCC TGATCCTGAG TTTGAGGCAA TTCTGGACAC TGTTGATCCC
    AACAGGGATG GCCACGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACG
    GAGAATGTCA AGTCCAGTGA AGAGATCGAG AGTGCTTTCC GGGCCCTCAG CTCTGAGGGC
    AAGCCTTATG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCTGACTAT
    TGTGTCTCCC ACATGAAGCC CTATGTGGAT GGCAAGGGCC GAGAACTTCC AACTGCCTTC
    GACTACGTCG AGTTCACCCG CTCACTCTTT GTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356254.1
    CDS230..7648
    Translation

    Target ORF information:

    RefSeq Version NM_001369325.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAGACAC GCATAACTAA GGAGGCCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGAG CAAGCACCAG AAGCACCAAG CCTTTGAGGC AGAACTGCAC
    GCCAATGCTG ACCGAATCCG TGGAGTTATC GACATGGGCA ACTCCCTCAT TGAGCGTGGG
    GCCTGTGCTG GCAGTGAGGA TGCTGTCAAG GCCCGCCTGG CTGCCCTTGC AGACCAGTGG
    CAGTTCCTGG TGCAGAAATC AGCTGAGAAG AGCCAGAAGC TAAAAGAGGC AAATAAGCAG
    CAGAACTTCA ACACTGGAAT CAAAGACTTT GACTTCTGGC TCTCTGAGGT GGAGGCTCTC
    CTGGCATCGG AAGATTATGG CAAAGACCTG GCTTCTGTGA ACAACTTGCT GAAAAAGCAT
    CAGCTGCTAG AGGCAGACAT CTCGGCCCAC GAGGATCGTC TGAAGGACCT GAACAGCCAG
    GCTGACAGCC TGATGACTAG CAGTGCCTTC GACACCTCCC AAGTGAAAGA GAAGCGGGAC
    ACCATCAATG GACGCTTCCA GAAGATCAAG AGCATGGCCA CCTCCCGAAG AGCGAAACTG
    AGCGAGTCCC ACCGCCTGCA CCAGTTCTTC CGGGACATGG ACGACGAGGA GTCCTGGATC
    AAGGAGAAGA AGTTGTTAGT GAGCTCTGAG GACTATGGCA GAGACCTCAC TGGTGTTCAG
    AATCTGAGGA AGAAACACAA GCGGCTAGAA GCTGAACTGG CTGCACATGA ACCCGCCATT
    CAGGGTGTCC TGGACACTGG GAAGAAGCTG TCTGATGACA ACACCATCGG GCAGGAGGAG
    ATCCAGCAAC GGCTTGCACA GTTTGTGGAG CACTGGAAGG AACTAAAGCA GCTGGCGGCT
    GCGCGGGGCC AGCGGCTAGA GGAGTCCTTG GAGTATCAGC AGTTTGTAGC CAACGTGGAA
    GAGGAGGAGG CTTGGATCAA TGAGAAGATG ACTCTGGTGG CCAGCGAAGA CTATGGAGAC
    ACTCTTGCTG CTATCCAGGG CTTACTGAAG AAACACGAAG CTTTTGAGAC AGACTTCACT
    GTCCACAAGG ATCGGGTGAA TGATGTCTGT ACTAATGGAC AAGACCTCAT TAAGAAGAAC
    AATCACCATG AGGAGAACAT CTCTTCAAAG ATGAAGGGTC TGAATGGAAA GGTGTCTGAT
    CTGGAGAAAG CAGCAGCTCA GAGGAAAGCA AAGCTGGATG AGAACTCAGC CTTCCTTCAG
    TTCAACTGGA AGGCTGACGT GGTAGAGTCC TGGATTGGTG AAAAGGAGAA CAGCTTGAAA
    ACAGATGACT ATGGCCGAGA TCTGTCTTCT GTCCAAACTC TGCTCACAAA ACAGGAGACA
    TTTGATGCTG GCCTGCAGGC CTTCCAGCAG GAGGGTATTG CCAATATCAC TGCCCTCAAA
    GACCAGCTGC TAGCTGCCAA GCACATTCAG TCTAAGGCCA TCGAGGCCCG ACATGCCTCC
    CTCATGAAGA GGTGGACCCA GCTGTTGGCC AATTCAGCTA CCCGGAAGAA GAAGTTGCTG
    GAGGCCCAGA GTCATTTCCG TAAGGTAGAA GACCTCTTCC TGACCTTTGC CAAAAAGGCA
    TCGGCTTTCA ATAGCTGGTT TGAGAATGCA GAGGAAGACC TCACAGACCC AGTGCGCTGC
    AACTCTCTGG AAGAAATCAA AGCCCTTCGA GAGGCTCACG ATGCCTTCCG CTCATCCCTC
    AGCTCTGCAC AGGCTGACTT CAACCAGCTA GCCGAGCTGG ACCGCCAGAT CAAGAGTTTC
    CGGGTGGCCT CCAACCCCTA TACCTGGTTC ACGATGGAGG CCCTGGAAGA GACCTGGAGA
    AACCTCCAGA AGATCATTAA GGAACGAGAA CTGGAGCTGC AGAAGGAACA GCGGCGGCAG
    GAGGAGAATG ACAAGCTGCG CCAAGAATTT GCCCAGCATG CCAACGCCTT CCACCAGTGG
    ATCCAGGAAA CAAGAACGTA TCTCCTCGAC GGGTCCTGCA TGGTGGAAGA GTCGGGAACT
    CTGGAATCTC AGCTTGAAGC TACCAAACGC AAGCACCAGG AGATTCGAGC CATGAGAAGT
    CAGCTGAAGA AGATCGAGGA CCTGGGGGCT GCCATGGAGG AAGCCCTCAT CCTGGACAAC
    AAGTACACGG AGCACAGCAC TGTGGGCCTG GCCCAGCAGT GGGACCAGTT AGACCAGCTG
    GGCATGCGCA TGCAGCACAA CCTGGAGCAG CAGATCCAGG CCAGGAACAC AACAGGAGTG
    ACTGAGGAGG CCCTCAAGGA ATTCAGCATG ATGTTCAAAC ACTTCGACAA GGACAAGTCT
    GGCCGGCTGA ACCATCAGGA GTTCAAATCC TGTCTTCGCT CCCTGGGCTA TGATCTGCCA
    ATGGTTGAGG AAGGAGAGCC TGATCCTGAG TTTGAGGCAA TTCTGGACAC TGTTGATCCC
    AACAGGGATG GCCACGTCTC CCTGCAAGAA TACATGGCTT TCATGATCAG CCGCGAAACG
    GAGAATGTCA AGTCCAGTGA AGAGATCGAG AGTGCTTTCC GGGCCCTCAG CTCTGAGGGC
    AAGCCTTATG TGACCAAGGA GGAGCTCTAC CAGAACCTGA CCCGGGAACA AGCTGACTAT
    TGTGTCTCCC ACATGAAGCC CTATGTGGAT GGCAAGGGCC GAGAACTTCC AACTGCCTTC
    GACTACGTCG AGTTCACCCG CTCACTCTTT GTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29394
    Clone ID Related Accession (Same CDS sequence) XM_006497813.3 , XM_006497813.4
    Accession Version XM_006497813.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497813.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497876.1
    CDS150..7619
    Translation

    Target ORF information:

    RefSeq Version XM_006497813.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497813.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29396
    Clone ID Related Accession (Same CDS sequence) XM_006497815.4 , XM_006497815.3
    Accession Version XM_006497815.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497815.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497878.1
    CDS150..7559
    Translation

    Target ORF information:

    RefSeq Version XM_006497815.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497815.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29397
    Clone ID Related Accession (Same CDS sequence) XM_006497816.3 , XM_006497816.4
    Accession Version XM_006497816.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGGT CCTGCATGGT GGAAGAGTCG GGAACTCTGG AATCTCAGCT TGAAGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TGAAGAAGAT CGAGGACCTG
    GGGGCTGCCA TGGAGGAAGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACTGTG
    GGCCTGGCCC AGCAGTGGGA CCAGTTAGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACTG AGGAGGCCCT CAAGGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCTGGCC GGCTGAACCA TCAGGAGTTC
    AAATCCTGTC TTCGCTCCCT GGGCTATGAT CTGCCAATGG TTGAGGAAGG AGAGCCTGAT
    CCTGAGTTTG AGGCAATTCT GGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCCCTG
    CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACGGAGA ATGTCAAGTC CAGTGAAGAG
    ATCGAGAGTG CTTTCCGGGC CCTCAGCTCT GAGGGCAAGC CTTATGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAACAAGCT GACTATTGTG TCTCCCACAT GAAGCCCTAT
    GTGGATGGCA AGGGCCGAGA ACTTCCAACT GCCTTCGACT ACGTCGAGTT CACCCGCTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497879.1
    CDS150..7544
    Translation

    Target ORF information:

    RefSeq Version XM_006497816.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497816.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGGT CCTGCATGGT GGAAGAGTCG GGAACTCTGG AATCTCAGCT TGAAGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TGAAGAAGAT CGAGGACCTG
    GGGGCTGCCA TGGAGGAAGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACTGTG
    GGCCTGGCCC AGCAGTGGGA CCAGTTAGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACTG AGGAGGCCCT CAAGGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCTGGCC GGCTGAACCA TCAGGAGTTC
    AAATCCTGTC TTCGCTCCCT GGGCTATGAT CTGCCAATGG TTGAGGAAGG AGAGCCTGAT
    CCTGAGTTTG AGGCAATTCT GGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCCCTG
    CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACGGAGA ATGTCAAGTC CAGTGAAGAG
    ATCGAGAGTG CTTTCCGGGC CCTCAGCTCT GAGGGCAAGC CTTATGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAACAAGCT GACTATTGTG TCTCCCACAT GAAGCCCTAT
    GTGGATGGCA AGGGCCGAGA ACTTCCAACT GCCTTCGACT ACGTCGAGTT CACCCGCTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19460
    Clone ID Related Accession (Same CDS sequence) NM_001177667.1
    Accession Version NM_001177667.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ054332.1, BC158015.1, BC150941.1 and CX206486.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 4. It encodes isoform 2, which is shorter than isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150941.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAGACAC GCATAACTAA GGAGGCCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG TTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATCGACAT GGGCAACTCC
    CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC
    CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAATCAGCTG AGAAGAGCCA GAAGCTAAAA
    GAGGCAAATA AGCAGCAGAA CTTCAACACT GGAATCAAAG ACTTTGACTT CTGGCTCTCT
    GAGGTGGAGG CTCTCCTGGC ATCGGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAAA AGCATCAGCT GCTAGAGGCA GACATCTCGG CCCACGAGGA TCGTCTGAAG
    GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAAGAGAAGC GGGACACCAT CAATGGACGC TTCCAGAAGA TCAAGAGCAT GGCCACCTCC
    CGAAGAGCGA AACTGAGCGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAC
    GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC
    CTCACTGGTG TTCAGAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCTGA ACTGGCTGCA
    CATGAACCCG CCATTCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAACACC
    ATCGGGCAGG AGGAGATCCA GCAACGGCTT GCACAGTTTG TGGAGCACTG GAAGGAACTA
    AAGCAGCTGG CGGCTGCGCG GGGCCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAACG TGGAAGAGGA GGAGGCTTGG ATCAATGAGA AGATGACTCT GGTGGCCAGC
    GAAGACTATG GAGACACTCT TGCTGCTATC CAGGGCTTAC TGAAGAAACA CGAAGCTTTT
    GAGACAGACT TCACTGTCCA CAAGGATCGG GTGAATGATG TCTGTACTAA TGGACAAGAC
    CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT
    GGAAAGGTGT CTGATCTGGA GAAAGCAGCA GCTCAGAGGA AAGCAAAGCT GGATGAGAAC
    TCAGCCTTCC TTCAGTTCAA CTGGAAGGCT GACGTGGTAG AGTCCTGGAT TGGTGAAAAG
    GAGAACAGCT TGAAAACAGA TGACTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC
    ACAAAACAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG TATTGCCAAT
    ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCTAA GGCCATCGAG
    GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGG
    AAGAAGAAGT TGCTGGAGGC CCAGAGTCAT TTCCGTAAGG TAGAAGACCT CTTCCTGACC
    TTTGCCAAAA AGGCATCGGC TTTCAATAGC TGGTTTGAGA ATGCAGAGGA AGACCTCACA
    GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TTCGAGAGGC TCACGATGCC
    TTCCGCTCAT CCCTCAGCTC TGCACAGGCT GACTTCAACC AGCTAGCCGA GCTGGACCGC
    CAGATCAAGA GTTTCCGGGT GGCCTCCAAC CCCTATACCT GGTTCACGAT GGAGGCCCTG
    GAAGAGACCT GGAGAAACCT CCAGAAGATC ATTAAGGAAC GAGAACTGGA GCTGCAGAAG
    GAACAGCGGC GGCAGGAGGA GAATGACAAG CTGCGCCAAG AATTTGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTG
    GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT
    CGAGCCATGA GAAGTCAGCT GAAGAAGATC GAGGACCTGG GGGCTGCCAT GGAGGAAGCC
    CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC
    CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACAACAG GAGTGACTGA GGAGGCCCTC AAGGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAT CAGGAGTTCA AATCCTGTCT TCGCTCCCTG
    GGCTATGATC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCAATTCTG
    GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCCCTGC AAGAATACAT GGCTTTCATG
    ATCAGCCGCG AAACGGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC
    CTCAGCTCTG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG
    GAACAAGCTG ACTATTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGAGAA
    CTTCCAACTG CCTTCGACTA CGTCGAGTTC ACCCGCTCAC TCTTTGTGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171138.1
    CDS230..7663
    Translation

    Target ORF information:

    RefSeq Version NM_001177667.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177667.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAGACAC GCATAACTAA GGAGGCCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG TTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATCGACAT GGGCAACTCC
    CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC
    CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAATCAGCTG AGAAGAGCCA GAAGCTAAAA
    GAGGCAAATA AGCAGCAGAA CTTCAACACT GGAATCAAAG ACTTTGACTT CTGGCTCTCT
    GAGGTGGAGG CTCTCCTGGC ATCGGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAAA AGCATCAGCT GCTAGAGGCA GACATCTCGG CCCACGAGGA TCGTCTGAAG
    GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAAGAGAAGC GGGACACCAT CAATGGACGC TTCCAGAAGA TCAAGAGCAT GGCCACCTCC
    CGAAGAGCGA AACTGAGCGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAC
    GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC
    CTCACTGGTG TTCAGAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCTGA ACTGGCTGCA
    CATGAACCCG CCATTCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAACACC
    ATCGGGCAGG AGGAGATCCA GCAACGGCTT GCACAGTTTG TGGAGCACTG GAAGGAACTA
    AAGCAGCTGG CGGCTGCGCG GGGCCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAACG TGGAAGAGGA GGAGGCTTGG ATCAATGAGA AGATGACTCT GGTGGCCAGC
    GAAGACTATG GAGACACTCT TGCTGCTATC CAGGGCTTAC TGAAGAAACA CGAAGCTTTT
    GAGACAGACT TCACTGTCCA CAAGGATCGG GTGAATGATG TCTGTACTAA TGGACAAGAC
    CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT
    GGAAAGGTGT CTGATCTGGA GAAAGCAGCA GCTCAGAGGA AAGCAAAGCT GGATGAGAAC
    TCAGCCTTCC TTCAGTTCAA CTGGAAGGCT GACGTGGTAG AGTCCTGGAT TGGTGAAAAG
    GAGAACAGCT TGAAAACAGA TGACTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC
    ACAAAACAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG TATTGCCAAT
    ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCTAA GGCCATCGAG
    GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGG
    AAGAAGAAGT TGCTGGAGGC CCAGAGTCAT TTCCGTAAGG TAGAAGACCT CTTCCTGACC
    TTTGCCAAAA AGGCATCGGC TTTCAATAGC TGGTTTGAGA ATGCAGAGGA AGACCTCACA
    GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TTCGAGAGGC TCACGATGCC
    TTCCGCTCAT CCCTCAGCTC TGCACAGGCT GACTTCAACC AGCTAGCCGA GCTGGACCGC
    CAGATCAAGA GTTTCCGGGT GGCCTCCAAC CCCTATACCT GGTTCACGAT GGAGGCCCTG
    GAAGAGACCT GGAGAAACCT CCAGAAGATC ATTAAGGAAC GAGAACTGGA GCTGCAGAAG
    GAACAGCGGC GGCAGGAGGA GAATGACAAG CTGCGCCAAG AATTTGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTG
    GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT
    CGAGCCATGA GAAGTCAGCT GAAGAAGATC GAGGACCTGG GGGCTGCCAT GGAGGAAGCC
    CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC
    CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACAACAG GAGTGACTGA GGAGGCCCTC AAGGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAT CAGGAGTTCA AATCCTGTCT TCGCTCCCTG
    GGCTATGATC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCAATTCTG
    GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCCCTGC AAGAATACAT GGCTTTCATG
    ATCAGCCGCG AAACGGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC
    CTCAGCTCTG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG
    GAACAAGCTG ACTATTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGAGAA
    CTTCCAACTG CCTTCGACTA CGTCGAGTTC ACCCGCTCAC TCTTTGTGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu19650
    Clone ID Related Accession (Same CDS sequence) NM_001177668.1
    Accession Version NM_001177668.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ054332.1, AK147317.1, BC172095.1 and CX206486.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 4. It encodes isoform 3, which is shorter than isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147317.1, BC172095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG TTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATCGACAT GGGCAACTCC
    CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC
    CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAATCAGCTG AGAAGAGCCA GAAGCTAAAA
    GAGGCAAATA AGCAGCAGAA CTTCAACACT GGAATCAAAG ACTTTGACTT CTGGCTCTCT
    GAGGTGGAGG CTCTCCTGGC ATCGGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAAA AGCATCAGCT GCTAGAGGCA GACATCTCGG CCCACGAGGA TCGTCTGAAG
    GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAAGAGAAGC GGGACACCAT CAATGGACGC TTCCAGAAGA TCAAGAGCAT GGCCACCTCC
    CGAAGAGCGA AACTGAGCGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAC
    GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC
    CTCACTGGTG TTCAGAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCTGA ACTGGCTGCA
    CATGAACCCG CCATTCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAACACC
    ATCGGGCAGG AGGAGATCCA GCAACGGCTT GCACAGTTTG TGGAGCACTG GAAGGAACTA
    AAGCAGCTGG CGGCTGCGCG GGGCCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAACG TGGAAGAGGA GGAGGCTTGG ATCAATGAGA AGATGACTCT GGTGGCCAGC
    GAAGACTATG GAGACACTCT TGCTGCTATC CAGGGCTTAC TGAAGAAACA CGAAGCTTTT
    GAGACAGACT TCACTGTCCA CAAGGATCGG GTGAATGATG TCTGTACTAA TGGACAAGAC
    CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT
    GGAAAGGTGT CTGATCTGGA GAAAGCAGCA GCTCAGAGGA AAGCAAAGCT GGATGAGAAC
    TCAGCCTTCC TTCAGTTCAA CTGGAAGGCT GACGTGGTAG AGTCCTGGAT TGGTGAAAAG
    GAGAACAGCT TGAAAACAGA TGACTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC
    ACAAAACAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG TATTGCCAAT
    ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCTAA GGCCATCGAG
    GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGG
    AAGAAGAAGT TGCTGGAGGC CCAGAGTCAT TTCCGTAAGG TAGAAGACCT CTTCCTGACC
    TTTGCCAAAA AGGCATCGGC TTTCAATAGC TGGTTTGAGA ATGCAGAGGA AGACCTCACA
    GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TTCGAGAGGC TCACGATGCC
    TTCCGCTCAT CCCTCAGCTC TGCACAGGCT GACTTCAACC AGCTAGCCGA GCTGGACCGC
    CAGATCAAGA GTTTCCGGGT GGCCTCCAAC CCCTATACCT GGTTCACGAT GGAGGCCCTG
    GAAGAGACCT GGAGAAACCT CCAGAAGATC ATTAAGGAAC GAGAACTGGA GCTGCAGAAG
    GAACAGCGGC GGCAGGAGGA GAATGACAAG CTGCGCCAAG AATTTGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTG
    GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT
    CGAGCCATGA GAAGTCAGCT GAAGAAGATC GAGGACCTGG GGGCTGCCAT GGAGGAAGCC
    CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC
    CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACAACAG GAGTGACTGA GGAGGCCCTC AAGGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAT CAGGAGTTCA AATCCTGTCT TCGCTCCCTG
    GGCTATGATC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCAATTCTG
    GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCCCTGC AAGAATACAT GGCTTTCATG
    ATCAGCCGCG AAACGGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC
    CTCAGCTCTG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG
    GAACAAGCTG ACTATTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGAGAA
    CTTCCAACTG CCTTCGACTA CGTCGAGTTC ACCCGCTCAC TCTTTGTGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171139.1
    CDS230..7603
    Translation

    Target ORF information:

    RefSeq Version NM_001177668.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177668.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG TTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATCGACAT GGGCAACTCC
    CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC
    CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAATCAGCTG AGAAGAGCCA GAAGCTAAAA
    GAGGCAAATA AGCAGCAGAA CTTCAACACT GGAATCAAAG ACTTTGACTT CTGGCTCTCT
    GAGGTGGAGG CTCTCCTGGC ATCGGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAAA AGCATCAGCT GCTAGAGGCA GACATCTCGG CCCACGAGGA TCGTCTGAAG
    GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAAGAGAAGC GGGACACCAT CAATGGACGC TTCCAGAAGA TCAAGAGCAT GGCCACCTCC
    CGAAGAGCGA AACTGAGCGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAC
    GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC
    CTCACTGGTG TTCAGAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCTGA ACTGGCTGCA
    CATGAACCCG CCATTCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAACACC
    ATCGGGCAGG AGGAGATCCA GCAACGGCTT GCACAGTTTG TGGAGCACTG GAAGGAACTA
    AAGCAGCTGG CGGCTGCGCG GGGCCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAACG TGGAAGAGGA GGAGGCTTGG ATCAATGAGA AGATGACTCT GGTGGCCAGC
    GAAGACTATG GAGACACTCT TGCTGCTATC CAGGGCTTAC TGAAGAAACA CGAAGCTTTT
    GAGACAGACT TCACTGTCCA CAAGGATCGG GTGAATGATG TCTGTACTAA TGGACAAGAC
    CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT
    GGAAAGGTGT CTGATCTGGA GAAAGCAGCA GCTCAGAGGA AAGCAAAGCT GGATGAGAAC
    TCAGCCTTCC TTCAGTTCAA CTGGAAGGCT GACGTGGTAG AGTCCTGGAT TGGTGAAAAG
    GAGAACAGCT TGAAAACAGA TGACTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC
    ACAAAACAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG TATTGCCAAT
    ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCTAA GGCCATCGAG
    GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGG
    AAGAAGAAGT TGCTGGAGGC CCAGAGTCAT TTCCGTAAGG TAGAAGACCT CTTCCTGACC
    TTTGCCAAAA AGGCATCGGC TTTCAATAGC TGGTTTGAGA ATGCAGAGGA AGACCTCACA
    GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TTCGAGAGGC TCACGATGCC
    TTCCGCTCAT CCCTCAGCTC TGCACAGGCT GACTTCAACC AGCTAGCCGA GCTGGACCGC
    CAGATCAAGA GTTTCCGGGT GGCCTCCAAC CCCTATACCT GGTTCACGAT GGAGGCCCTG
    GAAGAGACCT GGAGAAACCT CCAGAAGATC ATTAAGGAAC GAGAACTGGA GCTGCAGAAG
    GAACAGCGGC GGCAGGAGGA GAATGACAAG CTGCGCCAAG AATTTGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGGTC CTGCATGGTG
    GAAGAGTCGG GAACTCTGGA ATCTCAGCTT GAAGCTACCA AACGCAAGCA CCAGGAGATT
    CGAGCCATGA GAAGTCAGCT GAAGAAGATC GAGGACCTGG GGGCTGCCAT GGAGGAAGCC
    CTCATCCTGG ACAACAAGTA CACGGAGCAC AGCACTGTGG GCCTGGCCCA GCAGTGGGAC
    CAGTTAGACC AGCTGGGCAT GCGCATGCAG CACAACCTGG AGCAGCAGAT CCAGGCCAGG
    AACACAACAG GAGTGACTGA GGAGGCCCTC AAGGAATTCA GCATGATGTT CAAACACTTC
    GACAAGGACA AGTCTGGCCG GCTGAACCAT CAGGAGTTCA AATCCTGTCT TCGCTCCCTG
    GGCTATGATC TGCCAATGGT TGAGGAAGGA GAGCCTGATC CTGAGTTTGA GGCAATTCTG
    GACACTGTTG ATCCCAACAG GGATGGCCAC GTCTCCCTGC AAGAATACAT GGCTTTCATG
    ATCAGCCGCG AAACGGAGAA TGTCAAGTCC AGTGAAGAGA TCGAGAGTGC TTTCCGGGCC
    CTCAGCTCTG AGGGCAAGCC TTATGTGACC AAGGAGGAGC TCTACCAGAA CCTGACCCGG
    GAACAAGCTG ACTATTGTGT CTCCCACATG AAGCCCTATG TGGATGGCAA GGGCCGAGAA
    CTTCCAACTG CCTTCGACTA CGTCGAGTTC ACCCGCTCAC TCTTTGTGAA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68779
    Clone ID Related Accession (Same CDS sequence) NM_001309460.1
    Accession Version NM_001309460.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-30
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ054332.1, AK132224.1, AL928926.9, AK147317.1 and CX206486.1. On Jun 5, 2015 this sequence version replaced XM_006497811.2. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest protein (isoform 4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAGACAC GCATAACTAA GGAGGCCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG TTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATCGACAT GGGCAACTCC
    CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC
    CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAATCAGCTG AGAAGAGCCA GAAGCTAAAA
    GAGGCAAATA AGCAGCAGAA CTTCAACACT GGAATCAAAG ACTTTGACTT CTGGCTCTCT
    GAGGTGGAGG CTCTCCTGGC ATCGGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAAA AGCATCAGCT GCTAGAGGCA GACATCTCGG CCCACGAGGA TCGTCTGAAG
    GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAAGAGAAGC GGGACACCAT CAATGGACGC TTCCAGAAGA TCAAGAGCAT GGCCACCTCC
    CGAAGAGCGA AACTGAGCGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAC
    GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC
    CTCACTGGTG TTCAGAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCTGA ACTGGCTGCA
    CATGAACCCG CCATTCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAACACC
    ATCGGGCAGG AGGAGATCCA GCAACGGCTT GCACAGTTTG TGGAGCACTG GAAGGAACTA
    AAGCAGCTGG CGGCTGCGCG GGGCCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAACG TGGAAGAGGA GGAGGCTTGG ATCAATGAGA AGATGACTCT GGTGGCCAGC
    GAAGACTATG GAGACACTCT TGCTGCTATC CAGGGCTTAC TGAAGAAACA CGAAGCTTTT
    GAGACAGACT TCACTGTCCA CAAGGATCGG GTGAATGATG TCTGTACTAA TGGACAAGAC
    CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT
    GGAAAGGTGT CTGATCTGGA GAAAGCAGCA GCTCAGAGGA AAGCAAAGCT GGATGAGAAC
    TCAGCCTTCC TTCAGTTCAA CTGGAAGGCT GACGTGGTAG AGTCCTGGAT TGGTGAAAAG
    GAGAACAGCT TGAAAACAGA TGACTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC
    ACAAAACAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG TATTGCCAAT
    ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCTAA GGCCATCGAG
    GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGG
    AAGAAGAAGT TGCTGGAGGC CCAGAGTCAT TTCCGTAAGG TAGAAGACCT CTTCCTGACC
    TTTGCCAAAA AGGCATCGGC TTTCAATAGC TGGTTTGAGA ATGCAGAGGA AGACCTCACA
    GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TTCGAGAGGC TCACGATGCC
    TTCCGCTCAT CCCTCAGCTC TGCACAGGCT GACTTCAACC AGCTAGCCGA GCTGGACCGC
    CAGATCAAGA GTTTCCGGGT GGCCTCCAAC CCCTATACCT GGTTCACGAT GGAGGCCCTG
    GAAGAGACCT GGAGAAACCT CCAGAAGATC ATTAAGGAAC GAGAACTGGA GCTGCAGAAG
    GAACAGCGGC GGCAGGAGGA GAATGACAAG CTGCGCCAAG AATTTGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGCAT AGCATATCGT
    CGGGTCATTC GTGTCTGTCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAGT CGGGAACTCT GGAATCTCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAG
    ATTCGAGCCA TGAGAAGTCA GCTGAAGAAG ATCGAGGACC TGGGGGCTGC CATGGAGGAA
    GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACTG TGGGCCTGGC CCAGCAGTGG
    GACCAGTTAG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACAA CAGGAGTGAC TGAGGAGGCC CTCAAGGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CATCAGGAGT TCAAATCCTG TCTTCGCTCC
    CTGGGCTATG ATCTGCCAAT GGTTGAGGAA GGAGAGCCTG ATCCTGAGTT TGAGGCAATT
    CTGGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCCC TGCAAGAATA CATGGCTTTC
    ATGATCAGCC GCGAAACGGA GAATGTCAAG TCCAGTGAAG AGATCGAGAG TGCTTTCCGG
    GCCCTCAGCT CTGAGGGCAA GCCTTATGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC
    CGGGAACAAG CTGACTATTG TGTCTCCCAC ATGAAGCCCT ATGTGGATGG CAAGGGCCGA
    GAACTTCCAA CTGCCTTCGA CTACGTCGAG TTCACCCGCT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001296389.1
    CDS230..7726
    Translation

    Target ORF information:

    RefSeq Version NM_001309460.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001309460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGATCCAA GTGGGGTCAA AGTGCTGGAA ACAGCCGAGG ACATCCAGGA GAGACGACAG 
    CAAGTCCTGG ATCGGTACCA CCGCTTCAAG GAGCTCTCTA CCTTACGGCG TCAGAAGCTG
    GAGGATTCCT ATCGGTTCCA GTTTTTTCAA AGAGATGCTG AGGAGTTGGA GAAGTGGATT
    CAGGAGAAGC TTCAAGTTGC ATCTGATGAG AACTACAAAG ACCCAACCAA CTTGCAGGGA
    AAGCTGCAGA AGCACCAAGC CTTTGAAGCT GAAGTACAGG CTAACTCAGG AGCCATTGTG
    AAGCTGGACG AGACAGGAAA CTTGATGATT TCTGAAGGGC ACTTTGCATC TGAAACCATC
    CGGACACGTT TAATGGAGCT GCACCGGCAG TGGGAACTGC TTTTGGAGAA GATGCGGGAG
    AAAGGAATCA AACTGCTGCA GGCACAGAAG CTGGTGCAGT ATTTGCGGGA GTGTGAGGAT
    GTAATGGACT GGATCAATGA CAAGGAAGCA ATTGTGACTT CTGAGGAGCT GGGCCAGGAC
    CTGGAGCATG TAGAGGTGCT ACAGAAGAAG TTTGAAGAGT TTCAGACTGA TCTGGCTGCT
    CATGAAGAAA GAGTTAATGA AGTGAGCCAG TTTGCTGCCA AACTCATCCA GGAGCAGCAC
    CCGGAAGAGG AGCTGATCAA GACCAAGCAG GATGAGGTGA ATGCAGCATG GCAGCGACTG
    AAAGGCCTGG CTCTTCAAAG GCAGGGCAAG CTGTTCGGTG CTGCTGAGGT CCAGCGCTTT
    AACAGGGATG TAGATGAGAC CATTGGTTGG ATTAAGGAGA AAGAGCAGTT AATGGCCTCT
    GATGACTTCG GCAGAGACTT AGCAAGTGTT CAAGCTCTGC TTCGAAAGCA TGAGGGTCTG
    GAGAGAGATC TTGCTGCTCT AGAGGACAAG GTGAAAGCCC TGTGTGCTGA GGCTGACCGC
    CTGCAACAGT CACACCCTCT GAGTGCCAGC CAGATCCAGG TGAAGCGAGA GGAGCTGATT
    ACCAACTGGG AACAGATCCG AACTCTGGCA GCAGAGAGAC ATGCACGGCT TGATGACTCG
    TACAGGCTTC AGCGCTTTCT TGCTGACTTC CGTGACCTCA CAAGCTGGGT AACTGAAATG
    AAAGCCCTCA TCAATGCAGA TGAACTGGCC AATGATGTGG CTGGTGCTGA AGCCCTGCTG
    GACAGGCATC AAGAGCACAA GGGTGAGATC GACGCTCATG AAGATAGCTT TAAGTCTGCA
    GATGAGTCTG GGCAAGCTCT GCTTGCGGCT AGTCACTATG CCTCAGATGA AGTGAGGGAG
    AAGCTGAGCA TCCTCTCCGA GGAGAGAACT GCCCTGCTGG AGCTGTGGGA GCTTCGGAGG
    CAGCAGTATG AGCAGTGCAT GGACTTGCAG CTCTTCTACC GTGACACTGA GCAGGTGGAC
    AACTGGATGA GCAAGCAGGA GGCATTCCTG CTAAATGAAG ACTTGGGTGA CTCCTTAGAC
    AGTGTGGAAG CTCTTTTGAA GAAGCATGAG GACTTTGAAA AATCTCTCAG TGCCCAGGAA
    GAAAAAATCA CAGCCCTTGA TGAATTTGCA ACCAAACTCA TCCAAAATAA CCACTACGCA
    ATGGAAGATG TAGCGACTCG ACGAGATGCT CTCCTGAGCC GCCGCAATGC CCTTCATGAG
    CGAGCCATGC ATCGCCGGGC ACAGCTGGCT GATTCCTTCC ATCTGCAGCA GTTCTTCCGT
    GATTCTGATG AGCTCAAAAG TTGGGTCAAC GAGAAGATGA AAACGGCCAC CGATGAAGCT
    TATAAAGATC CATCCAACCT GCAAGGGAAA GTCCAAAAGC ATCAGGCTTT TGAGGCTGAG
    CTTTCAGCAA ACCAGAGCCG GATTGATGCC CTTGAGAAAG CTGGGCAGAA GCTAATAGAT
    GTCAACCACT ATGCCAAGGA AGAAGTAGCA GCTCGTATGA ATGAGGTCAT CAGTTTGTGG
    AAGAAACTTC TAGAGGCCAC AGAACTGAAA GGAATAAAGC TCCGAGAAGC CAACCAGCAG
    CAACAATTTA ATCGAAATGT TGAAGACATT GAATTGTGGC TGTATGAAGT AGAAGGTCAT
    TTGGCTTCAG ATGATTATGG TAAAGACCTC ACTAATGTCC AGAACCTCCA GAAGAAGCAT
    GCTCTGCTAG AGGCGGATGT TGCTGCTCAC CAGGATCGAA TTGATGGCAT CACAATTCAG
    GCCCGCCAGT TCCAAGATGC TGGCCATTTT GATGCCGAAA ATATTAAGAA GAAGCAAGAG
    GCCCTTGTGG CTCGCTATGA GGCTCTTAAG GAACCCATGG TGGCCCGGAA ACAGAAGCTG
    GCAGATTCTC TTCGTCTGCA GCAGCTCTTC CGGGATGTGG AGGACGAAGA AACCTGGATT
    CGAGAAAAGG AGCCTATTGC TGCATCCACT AACAGAGGCA AAGATCTTAT TGGAGTCCAG
    AATCTGCTAA AGAAACACCA AGCTTTGCAA GCAGAGATTG CTGGGCATGA ACCTCGAATC
    AAAGCAGTGA CACAAAAGGG AAATGCCATG GTGGAGGAAG GCCATTTTGC TGCAGAGGAT
    GTGAAGGCCA AACTGAGTGA GCTCAACCAG AAGTGGGAGG CTCTGAAAGC CAAAGCCTCC
    CAGCGGAGGC AGGATCTAGA GGACTCGCTG CAGGCCCAGC AGTACTTTGC CGATGCCAAT
    GAGGCTGAGT CCTGGATGCG GGAGAAGGAG CCCATTGTGG GCAGTACGGA CTATGGGAAG
    GATGAAGACT CTGCTGAGGC TCTACTCAAG AAGCATGAAG CTTTGATGTC CGATCTCAGC
    GCCTATGGCA GCAGCATCCA GGCTCTGCGA GAACAGGCCC AGTCATGCCG GCAACAAGTG
    GCCCCCATGG ATGATGAGAC TGGGAAGGAG CTAGTCTTGG CTCTCTATGA CTATCAAGAG
    AAGAGTCCTC GTGAGGTCAC CATGAAGAAA GGGGATATCC TCACCTTGCT CAACAGCACA
    AACAAGGATT GGTGGAAAGT GGAAGTGAAT GACCGGCAGG GTTTCGTGCC AGCTGCATAT
    GTGAAGAAGC TGGACCCCGC CCAGTCAGCC TCGAGGGAGA ACCTCCTAGA AGAGCAGGGC
    AGCATTGCTC TGCGGCAGGG GCAAATTGAC AACCAGACAC GCATAACTAA GGAGGCCGGC
    AGTGTATCTC TGCGTATGAA ACAGGTGGAA GAACTATATC AGTCTCTGCT GGAACTGGGT
    GAGAAGAGAA AAGGCATGTT GGAGAAGAGT TGCAAGAAGT TCATGTTGTT CCGGGAAGCA
    AACGAGTTAC AGCAGTGGAT CACTGAGAAG GAGGCTGCTC TAACCAATGA GGAGGTTGGC
    GCTGACTTGG AGCAGGTTGA GGTGCTGCAG AAGAAGTTTG ATGACTTCCA GAAGGATCTG
    AAAGCCAATG AGTCTCGGCT GAAGGACATT AACAAAGTAG CAGAAGACCT GGAGTCTGAA
    GGTCTGATGG CAGAGGAAGT GCAAGCTGTG CAGCAGCAGG AGGTGTATGG TGCGATGCCC
    AGGGATGAAG CAGATTCCAA GACGGCCTCC CCATGGAAGT CTGCTCGACT GATGGTCCAT
    ACAGTGGCTA CCTTCAACTC CATCAAGGAG CTGAATGAGC GTTGGCGGTC CCTGCAACAG
    CTGGCTGAGG AACGTAGCCA GCTCTTGGGC AGTGCACACG AAGTACAGAG GTTTCACAGA
    GATGCTGATG AAACTAAAGA ATGGATTGAA GAGAAGAATC AGGCTCTGAA CACAGACAAC
    TATGGCCATG ATTTAGCTAG TGTCCAGGCC CTGCAGCGCA AACATGAAGG CTTTGAGAGG
    GACCTTGCAG CTCTTGGTGA CAAGGTGAAT TCCCTTGGGG AAACAGCCCA AAGGCTGATC
    CAGTCCCACC CTGAATCTGC AGAGGACTTA AAGGAAAAGT GCACAGAGTT AAACCAGGCC
    TGGACCAGCC TAGGGAAGCG TGCAGACCAG CGCAAGGCCA AATTGGGTGA CTCCCATGAT
    CTGCAGCGCT TCCTTAGCGA TTTCCGGGAC CTCATGTCTT GGATCAATGG AATACGAGGG
    TTGGTATCTT CAGATGAACT AGCCAAGGAC GTCACTGGAG CTGAGGCTTT GCTGGAGCGA
    CACCAGGAAC ACCGGACAGA AATTGATGCC AGGGCTGGCA CTTTCCAGGC ATTTGAGCAG
    TTTGGGCAGC AGCTGTTAGC TCACGGGCAC TATGCCAGCC CGGAGATCAA AGAGAAACTT
    GATATTCTTG ACCAGGAGCG CACAGACCTG GAGAAGGCCT GGGTCCAGCG CAGGATGATG
    CTGGACCACT GCCTGGAGTT GCAGCTGTTC CATCGGGACT GTGAACAAGC AGAAAACTGG
    ATGGCTGCCC GGGAGGCCTT CCTAAACACA GAAGACAAAG GAGACTCGCT GGACAGTGTG
    GAGGCACTGA TCAAAAAACA TGAAGACTTT GACAAAGCTA TCAATGTCCA GGAAGAAAAG
    ATAGCTGCCC TGCAGGCCTT TGCCGACCAG CTCATTGCCG TTGACCACTA TGCCAAGGGA
    GACATTGCAA ACCGACGCAA TGAGGTCCTT GACCGGTGGC GCCGCCTAAA AGCCCAGATG
    ATTGAAAAAA GGTCAAAACT TGGAGAATCT CAAACACTTC AGCAGTTCAG CCGGGATGTG
    GATGAGATTG AAGCCTGGAT CAGTGAGAAG TTACAGACAG CCAGTGATGA GTCATACAAG
    GACCCCACCA ACATCCAGCT TTCCAAGCTG TTGAGCAAGC ACCAGAAGCA CCAAGCCTTT
    GAGGCAGAAC TGCACGCCAA TGCTGACCGA ATCCGTGGAG TTATCGACAT GGGCAACTCC
    CTCATTGAGC GTGGGGCCTG TGCTGGCAGT GAGGATGCTG TCAAGGCCCG CCTGGCTGCC
    CTTGCAGACC AGTGGCAGTT CCTGGTGCAG AAATCAGCTG AGAAGAGCCA GAAGCTAAAA
    GAGGCAAATA AGCAGCAGAA CTTCAACACT GGAATCAAAG ACTTTGACTT CTGGCTCTCT
    GAGGTGGAGG CTCTCCTGGC ATCGGAAGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC
    TTGCTGAAAA AGCATCAGCT GCTAGAGGCA GACATCTCGG CCCACGAGGA TCGTCTGAAG
    GACCTGAACA GCCAGGCTGA CAGCCTGATG ACTAGCAGTG CCTTCGACAC CTCCCAAGTG
    AAAGAGAAGC GGGACACCAT CAATGGACGC TTCCAGAAGA TCAAGAGCAT GGCCACCTCC
    CGAAGAGCGA AACTGAGCGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAC
    GAGGAGTCCT GGATCAAGGA GAAGAAGTTG TTAGTGAGCT CTGAGGACTA TGGCAGAGAC
    CTCACTGGTG TTCAGAATCT GAGGAAGAAA CACAAGCGGC TAGAAGCTGA ACTGGCTGCA
    CATGAACCCG CCATTCAGGG TGTCCTGGAC ACTGGGAAGA AGCTGTCTGA TGACAACACC
    ATCGGGCAGG AGGAGATCCA GCAACGGCTT GCACAGTTTG TGGAGCACTG GAAGGAACTA
    AAGCAGCTGG CGGCTGCGCG GGGCCAGCGG CTAGAGGAGT CCTTGGAGTA TCAGCAGTTT
    GTAGCCAACG TGGAAGAGGA GGAGGCTTGG ATCAATGAGA AGATGACTCT GGTGGCCAGC
    GAAGACTATG GAGACACTCT TGCTGCTATC CAGGGCTTAC TGAAGAAACA CGAAGCTTTT
    GAGACAGACT TCACTGTCCA CAAGGATCGG GTGAATGATG TCTGTACTAA TGGACAAGAC
    CTCATTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CAAAGATGAA GGGTCTGAAT
    GGAAAGGTGT CTGATCTGGA GAAAGCAGCA GCTCAGAGGA AAGCAAAGCT GGATGAGAAC
    TCAGCCTTCC TTCAGTTCAA CTGGAAGGCT GACGTGGTAG AGTCCTGGAT TGGTGAAAAG
    GAGAACAGCT TGAAAACAGA TGACTATGGC CGAGATCTGT CTTCTGTCCA AACTCTGCTC
    ACAAAACAGG AGACATTTGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG TATTGCCAAT
    ATCACTGCCC TCAAAGACCA GCTGCTAGCT GCCAAGCACA TTCAGTCTAA GGCCATCGAG
    GCCCGACATG CCTCCCTCAT GAAGAGGTGG ACCCAGCTGT TGGCCAATTC AGCTACCCGG
    AAGAAGAAGT TGCTGGAGGC CCAGAGTCAT TTCCGTAAGG TAGAAGACCT CTTCCTGACC
    TTTGCCAAAA AGGCATCGGC TTTCAATAGC TGGTTTGAGA ATGCAGAGGA AGACCTCACA
    GACCCAGTGC GCTGCAACTC TCTGGAAGAA ATCAAAGCCC TTCGAGAGGC TCACGATGCC
    TTCCGCTCAT CCCTCAGCTC TGCACAGGCT GACTTCAACC AGCTAGCCGA GCTGGACCGC
    CAGATCAAGA GTTTCCGGGT GGCCTCCAAC CCCTATACCT GGTTCACGAT GGAGGCCCTG
    GAAGAGACCT GGAGAAACCT CCAGAAGATC ATTAAGGAAC GAGAACTGGA GCTGCAGAAG
    GAACAGCGGC GGCAGGAGGA GAATGACAAG CTGCGCCAAG AATTTGCCCA GCATGCCAAC
    GCCTTCCACC AGTGGATCCA GGAAACAAGA ACGTATCTCC TCGACGGCAT AGCATATCGT
    CGGGTCATTC GTGTCTGTCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG
    GTGGAAGAGT CGGGAACTCT GGAATCTCAG CTTGAAGCTA CCAAACGCAA GCACCAGGAG
    ATTCGAGCCA TGAGAAGTCA GCTGAAGAAG ATCGAGGACC TGGGGGCTGC CATGGAGGAA
    GCCCTCATCC TGGACAACAA GTACACGGAG CACAGCACTG TGGGCCTGGC CCAGCAGTGG
    GACCAGTTAG ACCAGCTGGG CATGCGCATG CAGCACAACC TGGAGCAGCA GATCCAGGCC
    AGGAACACAA CAGGAGTGAC TGAGGAGGCC CTCAAGGAAT TCAGCATGAT GTTCAAACAC
    TTCGACAAGG ACAAGTCTGG CCGGCTGAAC CATCAGGAGT TCAAATCCTG TCTTCGCTCC
    CTGGGCTATG ATCTGCCAAT GGTTGAGGAA GGAGAGCCTG ATCCTGAGTT TGAGGCAATT
    CTGGACACTG TTGATCCCAA CAGGGATGGC CACGTCTCCC TGCAAGAATA CATGGCTTTC
    ATGATCAGCC GCGAAACGGA GAATGTCAAG TCCAGTGAAG AGATCGAGAG TGCTTTCCGG
    GCCCTCAGCT CTGAGGGCAA GCCTTATGTG ACCAAGGAGG AGCTCTACCA GAACCTGACC
    CGGGAACAAG CTGACTATTG TGTCTCCCAC ATGAAGCCCT ATGTGGATGG CAAGGGCCGA
    GAACTTCCAA CTGCCTTCGA CTACGTCGAG TTCACCCGCT CACTCTTTGT GAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29390
    Clone ID Related Accession (Same CDS sequence) XM_006497809.2
    Accession Version XM_006497809.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006497809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGCATAGCA TATCGTCGGG TCATTCGTGT CTGTCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAGTCGGG AACTCTGGAA
    TCTCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTG
    AAGAAGATCG AGGACCTGGG GGCTGCCATG GAGGAAGCCC TCATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACTGTGGG CCTGGCCCAG CAGTGGGACC AGTTAGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACTGAG
    GAGGCCCTCA AGGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCATC AGGAGTTCAA ATCCTGTCTT CGCTCCCTGG GCTATGATCT GCCAATGGTT
    GAGGAAGGAG AGCCTGATCC TGAGTTTGAG GCAATTCTGG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACGGAGAAT
    GTCAAGTCCA GTGAAGAGAT CGAGAGTGCT TTCCGGGCCC TCAGCTCTGA GGGCAAGCCT
    TATGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCTGA CTATTGTGTC
    TCCCACATGA AGCCCTATGT GGATGGCAAG GGCCGAGAAC TTCCAACTGC CTTCGACTAC
    GTCGAGTTCA CCCGCTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497872.1
    CDS150..7682
    Translation

    Target ORF information:

    RefSeq Version XM_006497809.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGCATAGCA TATCGTCGGG TCATTCGTGT CTGTCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAGTCGGG AACTCTGGAA
    TCTCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTG
    AAGAAGATCG AGGACCTGGG GGCTGCCATG GAGGAAGCCC TCATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACTGTGGG CCTGGCCCAG CAGTGGGACC AGTTAGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACTGAG
    GAGGCCCTCA AGGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCATC AGGAGTTCAA ATCCTGTCTT CGCTCCCTGG GCTATGATCT GCCAATGGTT
    GAGGAAGGAG AGCCTGATCC TGAGTTTGAG GCAATTCTGG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACGGAGAAT
    GTCAAGTCCA GTGAAGAGAT CGAGAGTGCT TTCCGGGCCC TCAGCTCTGA GGGCAAGCCT
    TATGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCTGA CTATTGTGTC
    TCCCACATGA AGCCCTATGT GGATGGCAAG GGCCGAGAAC TTCCAACTGC CTTCGACTAC
    GTCGAGTTCA CCCGCTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29391
    Clone ID Related Accession (Same CDS sequence) XM_006497810.2
    Accession Version XM_006497810.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006497810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGCA TAGCATATCG TCGGGTCATT CGTGTCTGTC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAG TCGGGAACTC TGGAATCTCA GCTTGAAGCT
    ACCAAACGCA AGCACCAGGA GATTCGAGCC ATGAGAAGTC AGCTGAAGAA GATCGAGGAC
    CTGGGGGCTG CCATGGAGGA AGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACT
    GTGGGCCTGG CCCAGCAGTG GGACCAGTTA GACCAGCTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGAGTGA CTGAGGAGGC CCTCAAGGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCTG GCCGGCTGAA CCATCAGGAG
    TTCAAATCCT GTCTTCGCTC CCTGGGCTAT GATCTGCCAA TGGTTGAGGA AGGAGAGCCT
    GATCCTGAGT TTGAGGCAAT TCTGGACACT GTTGATCCCA ACAGGGATGG CCACGTCTCC
    CTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACGG AGAATGTCAA GTCCAGTGAA
    GAGATCGAGA GTGCTTTCCG GGCCCTCAGC TCTGAGGGCA AGCCTTATGT GACCAAGGAG
    GAGCTCTACC AGAACCTGAC CCGGGAACAA GCTGACTATT GTGTCTCCCA CATGAAGCCC
    TATGTGGATG GCAAGGGCCG AGAACTTCCA ACTGCCTTCG ACTACGTCGA GTTCACCCGC
    TCACTCTTTG TGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497873.1
    CDS150..7667
    Translation

    Target ORF information:

    RefSeq Version XM_006497810.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497810.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGCA TAGCATATCG TCGGGTCATT CGTGTCTGTC AGTATGAAGT TGGGGATGAT
    CTGTCTGGAA GGTCCTGCAT GGTGGAAGAG TCGGGAACTC TGGAATCTCA GCTTGAAGCT
    ACCAAACGCA AGCACCAGGA GATTCGAGCC ATGAGAAGTC AGCTGAAGAA GATCGAGGAC
    CTGGGGGCTG CCATGGAGGA AGCCCTCATC CTGGACAACA AGTACACGGA GCACAGCACT
    GTGGGCCTGG CCCAGCAGTG GGACCAGTTA GACCAGCTGG GCATGCGCAT GCAGCACAAC
    CTGGAGCAGC AGATCCAGGC CAGGAACACA ACAGGAGTGA CTGAGGAGGC CCTCAAGGAA
    TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCTG GCCGGCTGAA CCATCAGGAG
    TTCAAATCCT GTCTTCGCTC CCTGGGCTAT GATCTGCCAA TGGTTGAGGA AGGAGAGCCT
    GATCCTGAGT TTGAGGCAAT TCTGGACACT GTTGATCCCA ACAGGGATGG CCACGTCTCC
    CTGCAAGAAT ACATGGCTTT CATGATCAGC CGCGAAACGG AGAATGTCAA GTCCAGTGAA
    GAGATCGAGA GTGCTTTCCG GGCCCTCAGC TCTGAGGGCA AGCCTTATGT GACCAAGGAG
    GAGCTCTACC AGAACCTGAC CCGGGAACAA GCTGACTATT GTGTCTCCCA CATGAAGCCC
    TATGTGGATG GCAAGGGCCG AGAACTTCCA ACTGCCTTCG ACTACGTCGA GTTCACCCGC
    TCACTCTTTG TGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29393
    Clone ID Related Accession (Same CDS sequence) XM_006497812.2
    Accession Version XM_006497812.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006497812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGCATAGCA TATCGTCGGG TCATTCGTGT CTGTCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAGTCGGG AACTCTGGAA
    TCTCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTG
    AAGAAGATCG AGGACCTGGG GGCTGCCATG GAGGAAGCCC TCATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACTGTGGG CCTGGCCCAG CAGTGGGACC AGTTAGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACTGAG
    GAGGCCCTCA AGGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCATC AGGAGTTCAA ATCCTGTCTT CGCTCCCTGG GCTATGATCT GCCAATGGTT
    GAGGAAGGAG AGCCTGATCC TGAGTTTGAG GCAATTCTGG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACGGAGAAT
    GTCAAGTCCA GTGAAGAGAT CGAGAGTGCT TTCCGGGCCC TCAGCTCTGA GGGCAAGCCT
    TATGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCTGA CTATTGTGTC
    TCCCACATGA AGCCCTATGT GGATGGCAAG GGCCGAGAAC TTCCAACTGC CTTCGACTAC
    GTCGAGTTCA CCCGCTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497875.1
    CDS150..7622
    Translation

    Target ORF information:

    RefSeq Version XM_006497812.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497812.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGCATAGCA TATCGTCGGG TCATTCGTGT CTGTCAGTAT
    GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAGTCGGG AACTCTGGAA
    TCTCAGCTTG AAGCTACCAA ACGCAAGCAC CAGGAGATTC GAGCCATGAG AAGTCAGCTG
    AAGAAGATCG AGGACCTGGG GGCTGCCATG GAGGAAGCCC TCATCCTGGA CAACAAGTAC
    ACGGAGCACA GCACTGTGGG CCTGGCCCAG CAGTGGGACC AGTTAGACCA GCTGGGCATG
    CGCATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACAACAGG AGTGACTGAG
    GAGGCCCTCA AGGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCTGGCCGG
    CTGAACCATC AGGAGTTCAA ATCCTGTCTT CGCTCCCTGG GCTATGATCT GCCAATGGTT
    GAGGAAGGAG AGCCTGATCC TGAGTTTGAG GCAATTCTGG ACACTGTTGA TCCCAACAGG
    GATGGCCACG TCTCCCTGCA AGAATACATG GCTTTCATGA TCAGCCGCGA AACGGAGAAT
    GTCAAGTCCA GTGAAGAGAT CGAGAGTGCT TTCCGGGCCC TCAGCTCTGA GGGCAAGCCT
    TATGTGACCA AGGAGGAGCT CTACCAGAAC CTGACCCGGG AACAAGCTGA CTATTGTGTC
    TCCCACATGA AGCCCTATGT GGATGGCAAG GGCCGAGAAC TTCCAACTGC CTTCGACTAC
    GTCGAGTTCA CCCGCTCACT CTTTGTGAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29394
    Clone ID Related Accession (Same CDS sequence) XM_006497813.3 , XM_006497813.4
    Accession Version XM_006497813.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497876.1
    CDS149..7618
    Translation

    Target ORF information:

    RefSeq Version XM_006497813.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497813.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29395
    Clone ID Related Accession (Same CDS sequence) XM_006497814.2
    Accession Version XM_006497814.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006497814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2281
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    2641
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    3181
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    4381
    4441
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    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGGT CCTGCATGGT GGAAGAGTCG GGAACTCTGG AATCTCAGCT TGAAGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TGAAGAAGAT CGAGGACCTG
    GGGGCTGCCA TGGAGGAAGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACTGTG
    GGCCTGGCCC AGCAGTGGGA CCAGTTAGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACTG AGGAGGCCCT CAAGGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCTGGCC GGCTGAACCA TCAGGAGTTC
    AAATCCTGTC TTCGCTCCCT GGGCTATGAT CTGCCAATGG TTGAGGAAGG AGAGCCTGAT
    CCTGAGTTTG AGGCAATTCT GGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCCCTG
    CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACGGAGA ATGTCAAGTC CAGTGAAGAG
    ATCGAGAGTG CTTTCCGGGC CCTCAGCTCT GAGGGCAAGC CTTATGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAACAAGCT GACTATTGTG TCTCCCACAT GAAGCCCTAT
    GTGGATGGCA AGGGCCGAGA ACTTCCAACT GCCTTCGACT ACGTCGAGTT CACCCGCTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497877.1
    CDS150..7604
    Translation

    Target ORF information:

    RefSeq Version XM_006497814.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497814.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AGACACGCAT AACTAAGGAG GCCGGCAGTG TATCTCTGCG TATGAAACAG
    GTGGAAGAAC TATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGGT CCTGCATGGT GGAAGAGTCG GGAACTCTGG AATCTCAGCT TGAAGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TGAAGAAGAT CGAGGACCTG
    GGGGCTGCCA TGGAGGAAGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACTGTG
    GGCCTGGCCC AGCAGTGGGA CCAGTTAGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACTG AGGAGGCCCT CAAGGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCTGGCC GGCTGAACCA TCAGGAGTTC
    AAATCCTGTC TTCGCTCCCT GGGCTATGAT CTGCCAATGG TTGAGGAAGG AGAGCCTGAT
    CCTGAGTTTG AGGCAATTCT GGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCCCTG
    CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACGGAGA ATGTCAAGTC CAGTGAAGAG
    ATCGAGAGTG CTTTCCGGGC CCTCAGCTCT GAGGGCAAGC CTTATGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAACAAGCT GACTATTGTG TCTCCCACAT GAAGCCCTAT
    GTGGATGGCA AGGGCCGAGA ACTTCCAACT GCCTTCGACT ACGTCGAGTT CACCCGCTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29396
    Clone ID Related Accession (Same CDS sequence) XM_006497815.4 , XM_006497815.3
    Accession Version XM_006497815.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497878.1
    CDS149..7558
    Translation

    Target ORF information:

    RefSeq Version XM_006497815.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497815.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGCTTTCC
    AAGCTGTTGA GCAAGCACCA GAAGCACCAA GCCTTTGAGG CAGAACTGCA CGCCAATGCT
    GACCGAATCC GTGGAGTTAT CGACATGGGC AACTCCCTCA TTGAGCGTGG GGCCTGTGCT
    GGCAGTGAGG ATGCTGTCAA GGCCCGCCTG GCTGCCCTTG CAGACCAGTG GCAGTTCCTG
    GTGCAGAAAT CAGCTGAGAA GAGCCAGAAG CTAAAAGAGG CAAATAAGCA GCAGAACTTC
    AACACTGGAA TCAAAGACTT TGACTTCTGG CTCTCTGAGG TGGAGGCTCT CCTGGCATCG
    GAAGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TGAAAAAGCA TCAGCTGCTA
    GAGGCAGACA TCTCGGCCCA CGAGGATCGT CTGAAGGACC TGAACAGCCA GGCTGACAGC
    CTGATGACTA GCAGTGCCTT CGACACCTCC CAAGTGAAAG AGAAGCGGGA CACCATCAAT
    GGACGCTTCC AGAAGATCAA GAGCATGGCC ACCTCCCGAA GAGCGAAACT GAGCGAGTCC
    CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGACGAGG AGTCCTGGAT CAAGGAGAAG
    AAGTTGTTAG TGAGCTCTGA GGACTATGGC AGAGACCTCA CTGGTGTTCA GAATCTGAGG
    AAGAAACACA AGCGGCTAGA AGCTGAACTG GCTGCACATG AACCCGCCAT TCAGGGTGTC
    CTGGACACTG GGAAGAAGCT GTCTGATGAC AACACCATCG GGCAGGAGGA GATCCAGCAA
    CGGCTTGCAC AGTTTGTGGA GCACTGGAAG GAACTAAAGC AGCTGGCGGC TGCGCGGGGC
    CAGCGGCTAG AGGAGTCCTT GGAGTATCAG CAGTTTGTAG CCAACGTGGA AGAGGAGGAG
    GCTTGGATCA ATGAGAAGAT GACTCTGGTG GCCAGCGAAG ACTATGGAGA CACTCTTGCT
    GCTATCCAGG GCTTACTGAA GAAACACGAA GCTTTTGAGA CAGACTTCAC TGTCCACAAG
    GATCGGGTGA ATGATGTCTG TACTAATGGA CAAGACCTCA TTAAGAAGAA CAATCACCAT
    GAGGAGAACA TCTCTTCAAA GATGAAGGGT CTGAATGGAA AGGTGTCTGA TCTGGAGAAA
    GCAGCAGCTC AGAGGAAAGC AAAGCTGGAT GAGAACTCAG CCTTCCTTCA GTTCAACTGG
    AAGGCTGACG TGGTAGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA AACAGATGAC
    TATGGCCGAG ATCTGTCTTC TGTCCAAACT CTGCTCACAA AACAGGAGAC ATTTGATGCT
    GGCCTGCAGG CCTTCCAGCA GGAGGGTATT GCCAATATCA CTGCCCTCAA AGACCAGCTG
    CTAGCTGCCA AGCACATTCA GTCTAAGGCC ATCGAGGCCC GACATGCCTC CCTCATGAAG
    AGGTGGACCC AGCTGTTGGC CAATTCAGCT ACCCGGAAGA AGAAGTTGCT GGAGGCCCAG
    AGTCATTTCC GTAAGGTAGA AGACCTCTTC CTGACCTTTG CCAAAAAGGC ATCGGCTTTC
    AATAGCTGGT TTGAGAATGC AGAGGAAGAC CTCACAGACC CAGTGCGCTG CAACTCTCTG
    GAAGAAATCA AAGCCCTTCG AGAGGCTCAC GATGCCTTCC GCTCATCCCT CAGCTCTGCA
    CAGGCTGACT TCAACCAGCT AGCCGAGCTG GACCGCCAGA TCAAGAGTTT CCGGGTGGCC
    TCCAACCCCT ATACCTGGTT CACGATGGAG GCCCTGGAAG AGACCTGGAG AAACCTCCAG
    AAGATCATTA AGGAACGAGA ACTGGAGCTG CAGAAGGAAC AGCGGCGGCA GGAGGAGAAT
    GACAAGCTGC GCCAAGAATT TGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAA
    ACAAGAACGT ATCTCCTCGA CGGGTCCTGC ATGGTGGAAG AGTCGGGAAC TCTGGAATCT
    CAGCTTGAAG CTACCAAACG CAAGCACCAG GAGATTCGAG CCATGAGAAG TCAGCTGAAG
    AAGATCGAGG ACCTGGGGGC TGCCATGGAG GAAGCCCTCA TCCTGGACAA CAAGTACACG
    GAGCACAGCA CTGTGGGCCT GGCCCAGCAG TGGGACCAGT TAGACCAGCT GGGCATGCGC
    ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CAACAGGAGT GACTGAGGAG
    GCCCTCAAGG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC TGGCCGGCTG
    AACCATCAGG AGTTCAAATC CTGTCTTCGC TCCCTGGGCT ATGATCTGCC AATGGTTGAG
    GAAGGAGAGC CTGATCCTGA GTTTGAGGCA ATTCTGGACA CTGTTGATCC CAACAGGGAT
    GGCCACGTCT CCCTGCAAGA ATACATGGCT TTCATGATCA GCCGCGAAAC GGAGAATGTC
    AAGTCCAGTG AAGAGATCGA GAGTGCTTTC CGGGCCCTCA GCTCTGAGGG CAAGCCTTAT
    GTGACCAAGG AGGAGCTCTA CCAGAACCTG ACCCGGGAAC AAGCTGACTA TTGTGTCTCC
    CACATGAAGC CCTATGTGGA TGGCAAGGGC CGAGAACTTC CAACTGCCTT CGACTACGTC
    GAGTTCACCC GCTCACTCTT TGTGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu29397
    Clone ID Related Accession (Same CDS sequence) XM_006497816.3 , XM_006497816.4
    Accession Version XM_006497816.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product spectrin alpha chain, non-erythrocytic 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGGT CCTGCATGGT GGAAGAGTCG GGAACTCTGG AATCTCAGCT TGAAGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TGAAGAAGAT CGAGGACCTG
    GGGGCTGCCA TGGAGGAAGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACTGTG
    GGCCTGGCCC AGCAGTGGGA CCAGTTAGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACTG AGGAGGCCCT CAAGGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCTGGCC GGCTGAACCA TCAGGAGTTC
    AAATCCTGTC TTCGCTCCCT GGGCTATGAT CTGCCAATGG TTGAGGAAGG AGAGCCTGAT
    CCTGAGTTTG AGGCAATTCT GGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCCCTG
    CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACGGAGA ATGTCAAGTC CAGTGAAGAG
    ATCGAGAGTG CTTTCCGGGC CCTCAGCTCT GAGGGCAAGC CTTATGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAACAAGCT GACTATTGTG TCTCCCACAT GAAGCCCTAT
    GTGGATGGCA AGGGCCGAGA ACTTCCAACT GCCTTCGACT ACGTCGAGTT CACCCGCTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006497879.1
    CDS149..7543
    Translation

    Target ORF information:

    RefSeq Version XM_006497816.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin alpha, non-erythrocytic 1 (Sptan1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006497816.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTGTCAT CGTTTCGTAA GCGCAGAGCT CAGAAAATGG ATCCAAGTGG GGTCAAAGTG 
    CTGGAAACAG CCGAGGACAT CCAGGAGAGA CGACAGCAAG TCCTGGATCG GTACCACCGC
    TTCAAGGAGC TCTCTACCTT ACGGCGTCAG AAGCTGGAGG ATTCCTATCG GTTCCAGTTT
    TTTCAAAGAG ATGCTGAGGA GTTGGAGAAG TGGATTCAGG AGAAGCTTCA AGTTGCATCT
    GATGAGAACT ACAAAGACCC AACCAACTTG CAGGGAAAGC TGCAGAAGCA CCAAGCCTTT
    GAAGCTGAAG TACAGGCTAA CTCAGGAGCC ATTGTGAAGC TGGACGAGAC AGGAAACTTG
    ATGATTTCTG AAGGGCACTT TGCATCTGAA ACCATCCGGA CACGTTTAAT GGAGCTGCAC
    CGGCAGTGGG AACTGCTTTT GGAGAAGATG CGGGAGAAAG GAATCAAACT GCTGCAGGCA
    CAGAAGCTGG TGCAGTATTT GCGGGAGTGT GAGGATGTAA TGGACTGGAT CAATGACAAG
    GAAGCAATTG TGACTTCTGA GGAGCTGGGC CAGGACCTGG AGCATGTAGA GGTGCTACAG
    AAGAAGTTTG AAGAGTTTCA GACTGATCTG GCTGCTCATG AAGAAAGAGT TAATGAAGTG
    AGCCAGTTTG CTGCCAAACT CATCCAGGAG CAGCACCCGG AAGAGGAGCT GATCAAGACC
    AAGCAGGATG AGGTGAATGC AGCATGGCAG CGACTGAAAG GCCTGGCTCT TCAAAGGCAG
    GGCAAGCTGT TCGGTGCTGC TGAGGTCCAG CGCTTTAACA GGGATGTAGA TGAGACCATT
    GGTTGGATTA AGGAGAAAGA GCAGTTAATG GCCTCTGATG ACTTCGGCAG AGACTTAGCA
    AGTGTTCAAG CTCTGCTTCG AAAGCATGAG GGTCTGGAGA GAGATCTTGC TGCTCTAGAG
    GACAAGGTGA AAGCCCTGTG TGCTGAGGCT GACCGCCTGC AACAGTCACA CCCTCTGAGT
    GCCAGCCAGA TCCAGGTGAA GCGAGAGGAG CTGATTACCA ACTGGGAACA GATCCGAACT
    CTGGCAGCAG AGAGACATGC ACGGCTTGAT GACTCGTACA GGCTTCAGCG CTTTCTTGCT
    GACTTCCGTG ACCTCACAAG CTGGGTAACT GAAATGAAAG CCCTCATCAA TGCAGATGAA
    CTGGCCAATG ATGTGGCTGG TGCTGAAGCC CTGCTGGACA GGCATCAAGA GCACAAGGGT
    GAGATCGACG CTCATGAAGA TAGCTTTAAG TCTGCAGATG AGTCTGGGCA AGCTCTGCTT
    GCGGCTAGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGAGCATCCT CTCCGAGGAG
    AGAACTGCCC TGCTGGAGCT GTGGGAGCTT CGGAGGCAGC AGTATGAGCA GTGCATGGAC
    TTGCAGCTCT TCTACCGTGA CACTGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCA
    TTCCTGCTAA ATGAAGACTT GGGTGACTCC TTAGACAGTG TGGAAGCTCT TTTGAAGAAG
    CATGAGGACT TTGAAAAATC TCTCAGTGCC CAGGAAGAAA AAATCACAGC CCTTGATGAA
    TTTGCAACCA AACTCATCCA AAATAACCAC TACGCAATGG AAGATGTAGC GACTCGACGA
    GATGCTCTCC TGAGCCGCCG CAATGCCCTT CATGAGCGAG CCATGCATCG CCGGGCACAG
    CTGGCTGATT CCTTCCATCT GCAGCAGTTC TTCCGTGATT CTGATGAGCT CAAAAGTTGG
    GTCAACGAGA AGATGAAAAC GGCCACCGAT GAAGCTTATA AAGATCCATC CAACCTGCAA
    GGGAAAGTCC AAAAGCATCA GGCTTTTGAG GCTGAGCTTT CAGCAAACCA GAGCCGGATT
    GATGCCCTTG AGAAAGCTGG GCAGAAGCTA ATAGATGTCA ACCACTATGC CAAGGAAGAA
    GTAGCAGCTC GTATGAATGA GGTCATCAGT TTGTGGAAGA AACTTCTAGA GGCCACAGAA
    CTGAAAGGAA TAAAGCTCCG AGAAGCCAAC CAGCAGCAAC AATTTAATCG AAATGTTGAA
    GACATTGAAT TGTGGCTGTA TGAAGTAGAA GGTCATTTGG CTTCAGATGA TTATGGTAAA
    GACCTCACTA ATGTCCAGAA CCTCCAGAAG AAGCATGCTC TGCTAGAGGC GGATGTTGCT
    GCTCACCAGG ATCGAATTGA TGGCATCACA ATTCAGGCCC GCCAGTTCCA AGATGCTGGC
    CATTTTGATG CCGAAAATAT TAAGAAGAAG CAAGAGGCCC TTGTGGCTCG CTATGAGGCT
    CTTAAGGAAC CCATGGTGGC CCGGAAACAG AAGCTGGCAG ATTCTCTTCG TCTGCAGCAG
    CTCTTCCGGG ATGTGGAGGA CGAAGAAACC TGGATTCGAG AAAAGGAGCC TATTGCTGCA
    TCCACTAACA GAGGCAAAGA TCTTATTGGA GTCCAGAATC TGCTAAAGAA ACACCAAGCT
    TTGCAAGCAG AGATTGCTGG GCATGAACCT CGAATCAAAG CAGTGACACA AAAGGGAAAT
    GCCATGGTGG AGGAAGGCCA TTTTGCTGCA GAGGATGTGA AGGCCAAACT GAGTGAGCTC
    AACCAGAAGT GGGAGGCTCT GAAAGCCAAA GCCTCCCAGC GGAGGCAGGA TCTAGAGGAC
    TCGCTGCAGG CCCAGCAGTA CTTTGCCGAT GCCAATGAGG CTGAGTCCTG GATGCGGGAG
    AAGGAGCCCA TTGTGGGCAG TACGGACTAT GGGAAGGATG AAGACTCTGC TGAGGCTCTA
    CTCAAGAAGC ATGAAGCTTT GATGTCCGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCT
    CTGCGAGAAC AGGCCCAGTC ATGCCGGCAA CAAGTGGCCC CCATGGATGA TGAGACTGGG
    AAGGAGCTAG TCTTGGCTCT CTATGACTAT CAAGAGAAGA GTCCTCGTGA GGTCACCATG
    AAGAAAGGGG ATATCCTCAC CTTGCTCAAC AGCACAAACA AGGATTGGTG GAAAGTGGAA
    GTGAATGACC GGCAGGGTTT CGTGCCAGCT GCATATGTGA AGAAGCTGGA CCCCGCCCAG
    TCAGCCTCGA GGGAGAACCT CCTAGAAGAG CAGGGCAGCA TTGCTCTGCG GCAGGGGCAA
    ATTGACAACC AATATCAGTC TCTGCTGGAA CTGGGTGAGA AGAGAAAAGG CATGTTGGAG
    AAGAGTTGCA AGAAGTTCAT GTTGTTCCGG GAAGCAAACG AGTTACAGCA GTGGATCACT
    GAGAAGGAGG CTGCTCTAAC CAATGAGGAG GTTGGCGCTG ACTTGGAGCA GGTTGAGGTG
    CTGCAGAAGA AGTTTGATGA CTTCCAGAAG GATCTGAAAG CCAATGAGTC TCGGCTGAAG
    GACATTAACA AAGTAGCAGA AGACCTGGAG TCTGAAGGTC TGATGGCAGA GGAAGTGCAA
    GCTGTGCAGC AGCAGGAGGT GTATGGTGCG ATGCCCAGGG ATGAAGCAGA TTCCAAGACG
    GCCTCCCCAT GGAAGTCTGC TCGACTGATG GTCCATACAG TGGCTACCTT CAACTCCATC
    AAGGAGCTGA ATGAGCGTTG GCGGTCCCTG CAACAGCTGG CTGAGGAACG TAGCCAGCTC
    TTGGGCAGTG CACACGAAGT ACAGAGGTTT CACAGAGATG CTGATGAAAC TAAAGAATGG
    ATTGAAGAGA AGAATCAGGC TCTGAACACA GACAACTATG GCCATGATTT AGCTAGTGTC
    CAGGCCCTGC AGCGCAAACA TGAAGGCTTT GAGAGGGACC TTGCAGCTCT TGGTGACAAG
    GTGAATTCCC TTGGGGAAAC AGCCCAAAGG CTGATCCAGT CCCACCCTGA ATCTGCAGAG
    GACTTAAAGG AAAAGTGCAC AGAGTTAAAC CAGGCCTGGA CCAGCCTAGG GAAGCGTGCA
    GACCAGCGCA AGGCCAAATT GGGTGACTCC CATGATCTGC AGCGCTTCCT TAGCGATTTC
    CGGGACCTCA TGTCTTGGAT CAATGGAATA CGAGGGTTGG TATCTTCAGA TGAACTAGCC
    AAGGACGTCA CTGGAGCTGA GGCTTTGCTG GAGCGACACC AGGAACACCG GACAGAAATT
    GATGCCAGGG CTGGCACTTT CCAGGCATTT GAGCAGTTTG GGCAGCAGCT GTTAGCTCAC
    GGGCACTATG CCAGCCCGGA GATCAAAGAG AAACTTGATA TTCTTGACCA GGAGCGCACA
    GACCTGGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ACCACTGCCT GGAGTTGCAG
    CTGTTCCATC GGGACTGTGA ACAAGCAGAA AACTGGATGG CTGCCCGGGA GGCCTTCCTA
    AACACAGAAG ACAAAGGAGA CTCGCTGGAC AGTGTGGAGG CACTGATCAA AAAACATGAA
    GACTTTGACA AAGCTATCAA TGTCCAGGAA GAAAAGATAG CTGCCCTGCA GGCCTTTGCC
    GACCAGCTCA TTGCCGTTGA CCACTATGCC AAGGGAGACA TTGCAAACCG ACGCAATGAG
    GTCCTTGACC GGTGGCGCCG CCTAAAAGCC CAGATGATTG AAAAAAGGTC AAAACTTGGA
    GAATCTCAAA CACTTCAGCA GTTCAGCCGG GATGTGGATG AGATTGAAGC CTGGATCAGT
    GAGAAGTTAC AGACAGCCAG TGATGAGTCA TACAAGGACC CCACCAACAT CCAGAGCAAG
    CACCAGAAGC ACCAAGCCTT TGAGGCAGAA CTGCACGCCA ATGCTGACCG AATCCGTGGA
    GTTATCGACA TGGGCAACTC CCTCATTGAG CGTGGGGCCT GTGCTGGCAG TGAGGATGCT
    GTCAAGGCCC GCCTGGCTGC CCTTGCAGAC CAGTGGCAGT TCCTGGTGCA GAAATCAGCT
    GAGAAGAGCC AGAAGCTAAA AGAGGCAAAT AAGCAGCAGA ACTTCAACAC TGGAATCAAA
    GACTTTGACT TCTGGCTCTC TGAGGTGGAG GCTCTCCTGG CATCGGAAGA TTATGGCAAA
    GACCTGGCTT CTGTGAACAA CTTGCTGAAA AAGCATCAGC TGCTAGAGGC AGACATCTCG
    GCCCACGAGG ATCGTCTGAA GGACCTGAAC AGCCAGGCTG ACAGCCTGAT GACTAGCAGT
    GCCTTCGACA CCTCCCAAGT GAAAGAGAAG CGGGACACCA TCAATGGACG CTTCCAGAAG
    ATCAAGAGCA TGGCCACCTC CCGAAGAGCG AAACTGAGCG AGTCCCACCG CCTGCACCAG
    TTCTTCCGGG ACATGGACGA CGAGGAGTCC TGGATCAAGG AGAAGAAGTT GTTAGTGAGC
    TCTGAGGACT ATGGCAGAGA CCTCACTGGT GTTCAGAATC TGAGGAAGAA ACACAAGCGG
    CTAGAAGCTG AACTGGCTGC ACATGAACCC GCCATTCAGG GTGTCCTGGA CACTGGGAAG
    AAGCTGTCTG ATGACAACAC CATCGGGCAG GAGGAGATCC AGCAACGGCT TGCACAGTTT
    GTGGAGCACT GGAAGGAACT AAAGCAGCTG GCGGCTGCGC GGGGCCAGCG GCTAGAGGAG
    TCCTTGGAGT ATCAGCAGTT TGTAGCCAAC GTGGAAGAGG AGGAGGCTTG GATCAATGAG
    AAGATGACTC TGGTGGCCAG CGAAGACTAT GGAGACACTC TTGCTGCTAT CCAGGGCTTA
    CTGAAGAAAC ACGAAGCTTT TGAGACAGAC TTCACTGTCC ACAAGGATCG GGTGAATGAT
    GTCTGTACTA ATGGACAAGA CCTCATTAAG AAGAACAATC ACCATGAGGA GAACATCTCT
    TCAAAGATGA AGGGTCTGAA TGGAAAGGTG TCTGATCTGG AGAAAGCAGC AGCTCAGAGG
    AAAGCAAAGC TGGATGAGAA CTCAGCCTTC CTTCAGTTCA ACTGGAAGGC TGACGTGGTA
    GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAAACAG ATGACTATGG CCGAGATCTG
    TCTTCTGTCC AAACTCTGCT CACAAAACAG GAGACATTTG ATGCTGGCCT GCAGGCCTTC
    CAGCAGGAGG GTATTGCCAA TATCACTGCC CTCAAAGACC AGCTGCTAGC TGCCAAGCAC
    ATTCAGTCTA AGGCCATCGA GGCCCGACAT GCCTCCCTCA TGAAGAGGTG GACCCAGCTG
    TTGGCCAATT CAGCTACCCG GAAGAAGAAG TTGCTGGAGG CCCAGAGTCA TTTCCGTAAG
    GTAGAAGACC TCTTCCTGAC CTTTGCCAAA AAGGCATCGG CTTTCAATAG CTGGTTTGAG
    AATGCAGAGG AAGACCTCAC AGACCCAGTG CGCTGCAACT CTCTGGAAGA AATCAAAGCC
    CTTCGAGAGG CTCACGATGC CTTCCGCTCA TCCCTCAGCT CTGCACAGGC TGACTTCAAC
    CAGCTAGCCG AGCTGGACCG CCAGATCAAG AGTTTCCGGG TGGCCTCCAA CCCCTATACC
    TGGTTCACGA TGGAGGCCCT GGAAGAGACC TGGAGAAACC TCCAGAAGAT CATTAAGGAA
    CGAGAACTGG AGCTGCAGAA GGAACAGCGG CGGCAGGAGG AGAATGACAA GCTGCGCCAA
    GAATTTGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAAACAAG AACGTATCTC
    CTCGACGGGT CCTGCATGGT GGAAGAGTCG GGAACTCTGG AATCTCAGCT TGAAGCTACC
    AAACGCAAGC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TGAAGAAGAT CGAGGACCTG
    GGGGCTGCCA TGGAGGAAGC CCTCATCCTG GACAACAAGT ACACGGAGCA CAGCACTGTG
    GGCCTGGCCC AGCAGTGGGA CCAGTTAGAC CAGCTGGGCA TGCGCATGCA GCACAACCTG
    GAGCAGCAGA TCCAGGCCAG GAACACAACA GGAGTGACTG AGGAGGCCCT CAAGGAATTC
    AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCTGGCC GGCTGAACCA TCAGGAGTTC
    AAATCCTGTC TTCGCTCCCT GGGCTATGAT CTGCCAATGG TTGAGGAAGG AGAGCCTGAT
    CCTGAGTTTG AGGCAATTCT GGACACTGTT GATCCCAACA GGGATGGCCA CGTCTCCCTG
    CAAGAATACA TGGCTTTCAT GATCAGCCGC GAAACGGAGA ATGTCAAGTC CAGTGAAGAG
    ATCGAGAGTG CTTTCCGGGC CCTCAGCTCT GAGGGCAAGC CTTATGTGAC CAAGGAGGAG
    CTCTACCAGA ACCTGACCCG GGAACAAGCT GACTATTGTG TCTCCCACAT GAAGCCCTAT
    GTGGATGGCA AGGGCCGAGA ACTTCCAACT GCCTTCGACT ACGTCGAGTT CACCCGCTCA
    CTCTTTGTGA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.