Spta1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spta1
Entrez Gene ID	20739
Full Name	spectrin alpha, erythrocytic 1
Synonyms	AF093576,AI451697,Spna-1,Spna1,ha,ihj,nmf4,sph
General protein information	Preferred Names spectrin alpha, erythrocytic 1 Names spectrin alpha chain, erythrocytic 1 alpha-spectrin 1, erythroid erythroid alpha-spectrin hemolytic anemia neuroscience mutagenesis facility, 4 spectrin alpha 1 spectrin alpha chain, erythrocyte spherocytosis
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 80.97 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011465.4	NP_035595.2	spectrin alpha chain, erythrocytic 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPTA1	NP_003117.2
	SPTA1	XP_001117115.1
Bos taurus (cattle)	SPTA1	NP_001193517.1
Mus musculus (house mouse)	Spta1	NP_035595.2
Pan troglodytes (chimpanzee)	SPTA1	XP_001169940.1
Canis lupus familiaris (dog)	SPTA1	XP_545733.3
Rattus norvegicus (Norway rat)	Spta1	NP_001011908.2

Gene Ontology
Bibliography

Related articles in PubMed

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports7(1)5164(2017 Jul)

A postprandial FGF19-SHP-LSD1 regulatory axis?mediates epigenetic repression of hepatic?autophagy.
Byun S, Kim YC, Zhang Y, Kong B, Guo G, Sadoshima J, Ma J, Kemper B, Kemper JK
The EMBO journal36(12)1755-1769(2017 06)

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Huang HM, Bauer DC, Lelliott PM, Greth A, McMorran BJ, Foote SJ, Burgio G
Scientific reports637197(2016 Nov)

?II-spectrin regulates invadosome stability and extracellular matrix degradation.
Ponceau A, Albig?s-Rizo C, Colin-Aronovicz Y, Destaing O, Lecomte MC
PloS one10(4)e0120781(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrates that the FGF19-SHP-LSD1 axis maintains homeostasis by suppressing unnecessary autophagic breakdown of cellular components, including lipids, under nutrient-rich postprandial conditions.
Title: A postprandial FGF19-SHP-LSD1 regulatory axis?mediates epigenetic repression of hepatic?autophagy.

Calcium plays a role in regulating the expression and function of beta-adducin to sustain normal organization of the spectrin-based cytoskeleton and the differentiation properties in keratinocytes through the calmodulin/EGFR/cadherin signaling pathway.
Title: ?-Adducin siRNA disruption of the spectrin-based cytoskeleton in differentiating keratinocytes prevented by calcium acting through calmodulin/epidermal growth factor receptor/cadherin pathway.

a new function for spectrins in the stability of invadosomes and the coupling between actin regulation and ECM degradation
Title: ?II-spectrin regulates invadosome stability and extracellular matrix degradation.

Results of this study provide evidence for a role for spectrin in different steps of megakaryocyte development through its participation in the formation of invaginated membranes and in the maintenance of proplatelet structure.
Title: The spectrin-based membrane skeleton stabilizes mouse megakaryocyte membrane systems and is essential for proplatelet and platelet formation.

organization of a spectrin-like cytoskeleton is associated with keratinocyte differentiation, and cytoskeleton disruption is mediated by either PKCdelta(Thr505) phosphorylation associated with phosphorylated adducin or due to reduction of endogenous adducin
Title: Disruption of spectrin-like cytoskeleton in differentiating keratinocytes by PKC? activation is associated with phosphorylated adducin.

The following Spta1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spta1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08206	NM_011465.4 Latest version!	Mus musculus spectrin alpha, erythrocytic 1 (Spta1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1770.30 $2529.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spta1 ( NM_011465.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035595.2 Mus musculus spectrin alpha, erythrocytic 1 (Spta1), mRNA.

Price & Availability↑

CloneID	OMu08206
Clone ID Related Accession (Same CDS sequence)	NM_011465.4
Accession Version	NM_011465.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	spectrin alpha chain, erythrocytic 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY005246.1, AF093576.1 and AK143823.1. On Mar 27, 2010 this sequence version replaced NM_011465.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF093576.1, U87455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGAAACTC CAAAGGAAAC TGCTGTCGAG AGCAGCGGAC CAAAGGTCCT AGAAACAGCA 
GAAGAAATCC AACACCGAAG AGCGGAGGTG TTAAACCAGT ACCAGAGGTT CAAGGATCGC 
GTTGCCGAGA GGGGTCAGAA GCTTGAGGAG TCTTACCACT ATCAAGTTTT CAGGAGAGAC 
GCAGATGACC TGGAGAAGTG GATCATGGAA AAGCTTGAAA TTGCAAAAGA TAAGACATAT 
GAACCCACAA ACATACAGGG GAAATATCAG AAGCACGAAT CCTTTGTATC AGAGGTGCAA 
GCCAAATCAA GAGTCCTTCC TGAGCTGGAG GAAATCAGGG AAGCTCGATT TGCAGAAGAC 
CATTTTGCCC ATGAAGCCAC CAAGACCCAT CTGAAGCAAC TTCGCCTGCT GTGGGATCTG 
CTCTTGGAGT TGACCCAAGA GAAAAGTGAC GTGCTGCTGC GGGCACTGAA GTTCTATCAA 
TATTCTCAGG AGTGTGAAGA CATCCTAGAG TGGGTCAAAG AGAAGGAAGC TATAGTGACA 
TTAGTGGAGC TGGGCGATGA CTGGGAGCGA ACTGAGGTCC TGCATAAGAA GTTTGAAGAA 
TTCCAGGAAG AACTGACCGC TCGGAAGGGA AAAGTGGACA GAGTGAACCA ATATGCTAAT 
GAGTGTGCCC AGGAGAAACA TCCTAAGTTA CCAGAGATAA AGGCAAAACA GGATGAAGTC 
AATGCTGCCT GGGATCGTCT CTGGAGCCTG GCTCTCAAGA GACGAGAAAG TCTGTCTAAT 
GCAGCAGACC TCCAGCGATT TAAAAGGGAT GTCAATGAAG CTATCCAGTG GATGGAGGAG 
AAGGAGCCGC AGCTCACCTC TGAGGACTAT GGCAAAGATC TTGTTAGTTC TGAGGCATTG 
TTTCACAATC ACAAGAGGCT CGAGAGGAAT CTCGCGGTCA TGGATGACAA GGTGAAGGAG 
TTGTGTGCCA AAGCAGACAA GCTGATGATT TCCCATTCTG CAGATGCACC TCAGATTCAA 
CAGATGAAAT TGGATCTAGT CTCCAACTGG GAACGCATCC GTGCCTTGGC CACTAACAGA 
TATGCAAAGC TGAAGGCTTC TTATGGGTAC CATCGGTTCT TATCTGACTA TGATGAGCTC 
TCAGGCTGGA TGAAGGAAAA GACTGCTCTG ATCAATGCTG ATGAGCTGCC AACAGATGTG 
GCCAGTGGAG AAGCCCTGCT AGCCAGGCAC CAGCAGCATA AGCATGAGAT TGACTCTTAT 
GATGATCGAT TTCAATCTGC TGATGCAACT GGTCAAGAAC TCCTGGATGG CAATCACGAA 
GCTTCTGAGG AAATTCGAGA GAAGATGACA ATACTTGCCA ATGACTGGGC TGCTCTGCTG 
GAACTGTGGG ACAAGTGTCA GCACCAATAC CGCCAATGTC TGGATTTCCA TCTCTTCTAC 
CGAGACAGTG AACAAGTGGA TAGTTGGATG AGCAGACAAG AGGCTTTCCT GGAAAATGAA 
GACCTGGGGA ACTCAGTGGG CAGCGTGGAA GCCCTCCTTC AGAAGCATGA TGACTTTGAA 
GAAGCGTTCA CTGCCCAGGA AGAAAAGATC ATAACCTTGG ATGAGACTGC AACCAAGCTT 
ATTGACAATG ACCACTATGA CTCTGAGAAT ATCGCTGCTA TTCGTGATGG GCTTTTGGCC 
AGACGTGATG CCCTGCGTGA AAGGGCTGCC ACTAGAAGAA AATTGTTGGT AGATTCACAA 
CTTCTTCAAC AACTGTATCA AGACTCAGAT GACCTAAAAA CCTGGATCAA TAAGAAGAAA 
AAGTTGGCTG ATGATGACGA TTACAAGGAT GTGCAGAACT TGAAGAGCCG GGTTCAGAAG 
CAGCAGGACT TTGAAGAGGA ATTGGCAGTG AATGAGATTA TGCTCAATAA CTTGGAGAAA 
ACAGGCCAGG AGATGATTGA GGATGGTCAC TACGCCTCTG AGGCTGTGGC TGCTCGCCTG 
AGTGAGGTTG CCAACCTCTG GAAGGAGTTG CTGGAGGCAA CAGCACAAAA AGGGACTCAG 
CTGTATGAAG CTAACCAGCT GCTACAGTTT GAGAACAATG CAGAAGACCT GAAGCGCTGG 
CTAGAGGAGG TGGAGTGGCA GGTTACCTCT GAGGATTATG GGAAAGGTCT GGCTGATGTA 
CAGAATCTAC TAAGAAAACA TGGCCTTCTA GAGTCAGATG TGACTGCTCG TCAGAATCAG 
GTGGACACCC TCACAGACAT GGCGGCACAC TTTGAAGAGA TAGGCCATCC CGACTCTGGG 
GACATCCGTG CAAGGCAGGA ATCCTTGCTT TCCCGTTTTG AGGCTCTGAA GGAGCCACTG 
GCCATTCGGA AGAAGAAGCT CATTGACCTA CTCAAGCTAC AGCAGATTTG CAGAGACTCG 
GAAGATGAGG AGGCGTGGAT CCAGGAGACT GAGCCCTCAG CAGCTTCCAC TCATCTTGGA 
AAGGACTTGG TTGCAGCCAA GAATCTTCTA AACAGACATG AAGTCATCCT GGCAGACATT 
GCGAGTCATG AACCACGGAT TCAAGTAATA ACAGAGAGGG GAAACAAAAT GGTGGAGGAA 
GGACACTTCG CTGCAGAAGA CATAGCATCT AGGGTTGAAA GCTTGAACAA AAATATGGAG 
TCTCTCCATG CTCGAGCTAT TAGACGGGAG AATGATCTTA AGGCCAATGT CCAGCTCCAA 
CAGTACCTGG CAGATCTGCA TGAAGCAGAA GCATGGATTA AAGAGAAAGA ACCCATTGTA 
GACAACAAAA ACTATGGAGC TGATGAAGAA GCAGCTGGGG CCCTTCTCAA GAAGCATGAA 
GCCTTCCTGG TGGATCTGAA TGCATTTGAA AACAGCATAA AGGCTCTAAG AGATCAGGCA 
GAAGTCTGCC AGCAACAGCA GGCAGCACCA GTGGACGAGG CTGGTCGAGA AGCTAGGGTC 
ATAGCTTTGT ATGACTTTGA GGCTCGCAGC CGTAGAGAGG TCAGCATGAA GAAGAATGAT 
GTCTTAACTC TGCTCAGTTC CATCAACAAG GACTGGTGGA AGGTTGAAGC TGATGATCAC 
CAGGGCTTTG TGCCAGCGGT TTATGTCAGG AAACTGGCCC CCGATGAGCT ACCAGGTTTC 
CCACAGCATC GGCAAGAGGA GCCAGTCAAC ATCCCTCAGC TCCAGCAGCA GGTCGAGACC 
CTGTACCACT CCCTCCTAGA TCGGGCAGAA GAGCGCAGAC GTCGCTTGCT GCAGCGCTAC 
AATGAGTTTC TGCTGGCCTA TGAGGCAGGA GACATGTTGG AGTGGATCCA AGAGAAAAAG 
ACAGAAAACA CTGGAGTGGA ACTGGATGAT GTTTGGGAAC TGCAGAAGAA GTTTGACGAG 
TTCCAGAGGG ATCTAAAGAG TAATGAGCCT CGGCTAAAGG ACATCAACAA AGTGGCTGAC 
GAACTGCTCT TTGAAGAGCT CCTAACCCCA GAAGGAGCTC ACATTCGACA GGAGTTGAAC 
ACCCGCTGGA ATTCCCTGAA GAGGCTGGCA GATGAACAGT ATCAGCTGCT CAGCAGTGCA 
CATGCTGTTG AGATGTTTCA CAGGGAAGCA GATGATGTCA AGGAACAGAT TGATAAGAAA 
TGCCGGGCTC TCAATGCTGC CGACCCTGGC TCTGACCTGC TCAGTGTCCA GGCCCTCCAG 
AGGCAACATG AAGTCTTTGA GAGAGACATC ATCCCCCTGG GAGAAAAGGT GACTACGCTG 
GGGGAGACTG CTGAGCGTCT CTGTGAATCA CACCCAGATG CCACTGAGGA CCTACAGAAG 
CAGAGAACAG AGCTGAATGA GGCCTGGGAC ACACTTCAAG GTCTTACAAG TGATCGGAAG 
GAGAGTCTGA ATGAAGCCCA TAAATTCTTC CTCTTCCTTA GCAAGGCCAG TGATCTAGAG 
AACTGGATCA AAACCATTGG CGGTGTGATA TCATCACCTG AGCTGGCTGA GGACTTAACT 
GGCACAGAGA TCTTGCTGGA GCGACACCAG GAGCATCACG ATGACATAAA GAGAGAGGAC 
CCTACCTTCC AGGCCTTAGA AGACTTTGGT ACAGAACTTA TAGACAGCGG CCACCGTAAC 
AGACGTGAAA TTGACAATAC TCTCCAGAAT ATTAATTCAA AGAGAGACAA TTTGGAAAAG 
AGTTGGGAAA ACCGCAAGAA GATGCTAGAC CAGTGCTTAG AACTGCAGTT ATTCCGAGGA 
AAGTGTGACC AGGTCGAGAG CTGGATGGTG GCCCGTGAGA ATTCCCTGAG GTCAGACGAC 
AGGGATCACT TAAACAGTCT GCAGGCTTTG ATGAAGAAAC GGGATGATTT GGACAAAGCA 
ATCACTGCCC AGGAAGGGAA GATTTCGGAC CTAGAGAACG TTGCCACAAG GCTCATAGAC 
AATGACCACT ATGCCAAGGA AGAGATTGCT GCTCGCCTCC AACGTGTGCT AGACAGGTGG 
AAGGCTCTCA AAGAACAGCT GCTTACTGAA CTGGGAAAGC TAGGAGACTA TGCTGACCTC 
AAACAGTTCT ATCGTGACCT TGAGGACCTG GAGGAATGGA TCAATGAGAT GCTTCCCATA 
GCCTGTGATG AATCTTATAA AGATCCCACT AACATTCAGA GGAAATATCT GAAGCATCAA 
GCCTTTGAAA ATGAAGTCAA CGGTCGTGCT GAGCAGGTGG ATGGGGTTAT CAACCTTGGG 
AATTCCCTGA TTGAGCGCAG AGTGTGTGAT GGAGATGAAG AGAACATGCA GGAGCAACTG 
GACAAGCTAA AGGAGAACTG GGATTATCTC CTAGAGAGAA CCACTGACAA AGGACAGAAG 
CTCAACGAGG CCAGTCGGCA GCAAAGGTTC AACACAAGCA TCCGGGACTT TGAGTTCTGG 
CTTTCAGAGG CAGAAGGCTT GTTGGCCATG AAGGATCAGG CCAGGGACTT AACTTCAGCA 
GGAAACCTAC TTAAGAAGCA TCAGCTATTG GAAGCAGAGA TGTTGGCACG AGAGGACCCT 
CTCAAAGATC TGAATGACCT GGCTCAGGAG CTGATCTCCA GTGGGACTTT CAACATTGAC 
CAGATAGAAG AGAAAATGAA TGGTGTCAAT GAGCGCTTTG AAAATGTCCA GAGCTTGGCA 
GCTGCACACC ACGAGAAGCT GAAAGAGACG TATGCCTTGT TCCAGTTCTT CCAAGACCTG 
GATGATGAGG AAGCCTGGAT AGAGGAGAAG TTGTTACGAG TGAGCTCCCA GGACTATGGG 
AGGGATCTTC AGAGTGTTCA GAACTTATTG AAAAAACACA AACGCCTAGA GGGGGAGCTA 
GTGGCACATG AACCTGCTGT CCAGAATGTG CTGGATACGG CAGAAAGCCT GAGAGACAAG 
GCTGCTGTAG GGAAAGAGGA GATCCAGGAG CGGCTGGCTC AGTTTGTCCA ACACTGGGAA 
AAGCTCAAAG AGTTGGCGAA GACCCGAGGG GTGAACTTGG AGGAGTCCCT CGAGTATCTG 
CAGTTCATGG AGAATGCAGA AGAAGAGGAA GCATGGCTGG GGGAGAAGTG TGCTCTGGTC 
AGTCGCGGGG ATTCAGGAGA CACACTGGCT GCTACTCAGA GTTTGCTAAA GAAGCATGAA 
GCTTTGGAGA ATGACTTTGC TGTGCACAAG AACCGGGTAC AAGATGTTTG TGCACAAGGA 
GAAGACATTC TTAATAAGGA AGAAACTCAG AACAAGGATA AGATTTCCAC CAAGATCCAA 
GTTCTGAATG AAAAGACAGC CTCTTTGGCC AAGGCATTAG CTGCTTGGAA GTCACAACTG 
GACGATGTGC ATGCCTTTCA GCAGTTTAAC TGGAAGGCGG ACGTAGTGGA ATCCTGGATA 
GGTGAGAAAG AAGCAAGCCT GAAGACCAAG AGCAACGGTG CAGACCTCAC CGCCTTCCTC 
ACACTACTGG CAAAGCACGA CACACTGGAT GCCAGTCTTC AGAGCTTCCA GCAAGAAAGG 
CTTTCTGAAA TCGCTGAGCT GAAGGACCAG CTTGTGGCTG GTGAGCACAG CCAAGCCAAA 
GCCATTGAGG AGCAGCATGC AGCCTTGCTG AGGCACTGGG AACAACTGCT GGAGGCATCT 
AGAGTTCACA GGCAGAAACT GCTGGAAAAG CAGCTGCCTC TACAGAAGGC TGAGGAGCTA 
TTCATGGAAT TTGCACACAA AGCTTCAGCC TTCAACAACT GGTGTGAGAA CGCGGAAGAG 
GACTTGTCAG AACCTGTGCA TTGTGTCTCT CTAAATGAGA TCCGGCAGCT GCAGAAGGAA 
CATGAAGCCT TCTTGGCCTC TCTGGCTGGG GCTCAAGAGG ATTTCAACTA TTTGTTGGAG 
CTAGACAAGC AAATTAAAGC CTTAAATGTC CCCTCCAGTC CTTATACCTG GTTAACAGTG 
GATGTGCTGG GCAGAATCTG GAACCACCTG CCAGACATCA TCAAGGAAAG GGAACAGGAA 
CTACAGAAAG AAGAGGCTAG GCAGATCAAG AACTTTGAAA TGTGCCAGGA GTTTGAACAA 
AATGCCAGTG CCTTCCTTCA GTGGATCCAG GAGACCAGGG CCTATTTTCT GGATGGATCA 
TTGCTCAAGG AAACAGGAAC TCTGGAATCC CAACTTGAAG CAAATAAAAG GAAACAGAAG 
GAGATCCAGG CAATGAAGCG CCATCTGACC AAGATTGAAG ACCTGGGGGA CAGCATGGAA 
GAGGCTCTGA TCCTTGATAT CAAATACAGC ACCATTGGAC TGGCCCAGCA ATGGGATCAG 
CTGCACCAAC TAGGGATGCG AATGCAACAC AATTTGGAGC AACAGATTCA AGCCAAAGAC 
ACCATAGGTG TGAGTGAGGA GACATTGAAA GAATTTAGCA CCACATACAA ACACTTCGAT 
GAGAACTTGA CAGGGCGATT GACTCACAAA GAGTTCCGCT CTTGCCTGAG AGGACTCAAT 
TACTATCTGC CTATGGTAGA GGAAGGTGAA CCTGAGCCCA AGTTTGAGAA ATTCCTGAAT 
GCTGTGGATC CAGGGAGGAA AGGATATGTT TCACTGGAAG ACTATACATC ATTCCTGATT 
GACAAGGAGT CAGAGAATAT CAAGACCAGC GATGACATAG AGAGTGCATT CCAAGCTCTG 
GCAGAAGGCA AGGCCTATAT TACCAAGGAG GACATGAAAC AGGCTCTCAC CCCGGAACAA 
GTGTCATTCT GTACCATACA TATGCAGCAA TATATGGATC CACGGGGTCG GAGCCAACCT 
GCTGGCTACG ACTATGTTGG CTTCACCAAT TCATTCTTTG GCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035595.2
CDS	166..7413
Translation	METPKETAVESSGPKVLETAEEIQHRRAEVLNQYQRFKDRVAERGQKLEESYHYQVFRRDADDLEKWIMEKLEIAKDKTYEPTNIQGKYQKHESFVSEVQAKSRVLPELEEIREARFAEDHFAHEATKTHLKQLRLLWDLLLELTQEKSDVLLRALKFYQYSQECEDILEWVKEKEAIVTLVELGDDWERTEVLHKKFEEFQEELTARKGKVDRVNQYANECAQEKHPKLPEIKAKQDEVNAAWDRLWSLALKRRESLSNAADLQRFKRDVNEAIQWMEEKEPQLTSEDYGKDLVSSEALFHNHKRLERNLAVMDDKVKELCAKADKLMISHSADAPQIQQMKLDLVSNWERIRALATNRYAKLKASYGYHRFLSDYDELSGWMKEKTALINADELPTDVASGEALLARHQQHKHEIDSYDDRFQSADATGQELLDGNHEASEEIREKMTILANDWAALLELWDKCQHQYRQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSVGSVEALLQKHDDFEEAFTAQEEKIITLDETATKLIDNDHYDSENIAAIRDGLLARRDALRERAATRRKLLVDSQLLQQLYQDSDDLKTWINKKKKLADDDDYKDVQNLKSRVQKQQDFEEELAVNEIMLNNLEKTGQEMIEDGHYASEAVAARLSEVANLWKELLEATAQKGTQLYEANQLLQFENNAEDLKRWLEEVEWQVTSEDYGKGLADVQNLLRKHGLLESDVTARQNQVDTLTDMAAHFEEIGHPDSGDIRARQESLLSRFEALKEPLAIRKKKLIDLLKLQQICRDSEDEEAWIQETEPSAASTHLGKDLVAAKNLLNRHEVILADIASHEPRIQVITERGNKMVEEGHFAAEDIASRVESLNKNMESLHARAIRRENDLKANVQLQQYLADLHEAEAWIKEKEPIVDNKNYGADEEAAGALLKKHEAFLVDLNAFENSIKALRDQAEVCQQQQAAPVDEAGREARVIALYDFEARSRREVSMKKNDVLTLLSSINKDWWKVEADDHQGFVPAVYVRKLAPDELPGFPQHRQEEPVNIPQLQQQVETLYHSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKTENTGVELDDVWELQKKFDEFQRDLKSNEPRLKDINKVADELLFEELLTPEGAHIRQELNTRWNSLKRLADEQYQLLSSAHAVEMFHREADDVKEQIDKKCRALNAADPGSDLLSVQALQRQHEVFERDIIPLGEKVTTLGETAERLCESHPDATEDLQKQRTELNEAWDTLQGLTSDRKESLNEAHKFFLFLSKASDLENWIKTIGGVISSPELAEDLTGTEILLERHQEHHDDIKREDPTFQALEDFGTELIDSGHRNRREIDNTLQNINSKRDNLEKSWENRKKMLDQCLELQLFRGKCDQVESWMVARENSLRSDDRDHLNSLQALMKKRDDLDKAITAQEGKISDLENVATRLIDNDHYAKEEIAARLQRVLDRWKALKEQLLTELGKLGDYADLKQFYRDLEDLEEWINEMLPIACDESYKDPTNIQRKYLKHQAFENEVNGRAEQVDGVINLGNSLIERRVCDGDEENMQEQLDKLKENWDYLLERTTDKGQKLNEASRQQRFNTSIRDFEFWLSEAEGLLAMKDQARDLTSAGNLLKKHQLLEAEMLAREDPLKDLNDLAQELISSGTFNIDQIEEKMNGVNERFENVQSLAAAHHEKLKETYALFQFFQDLDDEEAWIEEKLLRVSSQDYGRDLQSVQNLLKKHKRLEGELVAHEPAVQNVLDTAESLRDKAAVGKEEIQERLAQFVQHWEKLKELAKTRGVNLEESLEYLQFMENAEEEEAWLGEKCALVSRGDSGDTLAATQSLLKKHEALENDFAVHKNRVQDVCAQGEDILNKEETQNKDKISTKIQVLNEKTASLAKALAAWKSQLDDVHAFQQFNWKADVVESWIGEKEASLKTKSNGADLTAFLTLLAKHDTLDASLQSFQQERLSEIAELKDQLVAGEHSQAKAIEEQHAALLRHWEQLLEASRVHRQKLLEKQLPLQKAEELFMEFAHKASAFNNWCENAEEDLSEPVHCVSLNEIRQLQKEHEAFLASLAGAQEDFNYLLELDKQIKALNVPSSPYTWLTVDVLGRIWNHLPDIIKEREQELQKEEARQIKNFEMCQEFEQNASAFLQWIQETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRHLTKIEDLGDSMEEALILDIKYSTIGLAQQWDQLHQLGMRMQHNLEQQIQAKDTIGVSEETLKEFSTTYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEGEPEPKFEKFLNAVDPGRKGYVSLEDYTSFLIDKESENIKTSDDIESAFQALAEGKAYITKEDMKQALTPEQVSFCTIHMQQYMDPRGRSQPAGYDYVGFTNSFFGN

Target ORF information:

RefSeq Version	NM_011465.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus spectrin alpha, erythrocytic 1 (Spta1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011465.4

ORF Insert Sequence:

ATGGAAACTC CAAAGGAAAC TGCTGTCGAG AGCAGCGGAC CAAAGGTCCT AGAAACAGCA 
GAAGAAATCC AACACCGAAG AGCGGAGGTG TTAAACCAGT ACCAGAGGTT CAAGGATCGC 
GTTGCCGAGA GGGGTCAGAA GCTTGAGGAG TCTTACCACT ATCAAGTTTT CAGGAGAGAC 
GCAGATGACC TGGAGAAGTG GATCATGGAA AAGCTTGAAA TTGCAAAAGA TAAGACATAT 
GAACCCACAA ACATACAGGG GAAATATCAG AAGCACGAAT CCTTTGTATC AGAGGTGCAA 
GCCAAATCAA GAGTCCTTCC TGAGCTGGAG GAAATCAGGG AAGCTCGATT TGCAGAAGAC 
CATTTTGCCC ATGAAGCCAC CAAGACCCAT CTGAAGCAAC TTCGCCTGCT GTGGGATCTG 
CTCTTGGAGT TGACCCAAGA GAAAAGTGAC GTGCTGCTGC GGGCACTGAA GTTCTATCAA 
TATTCTCAGG AGTGTGAAGA CATCCTAGAG TGGGTCAAAG AGAAGGAAGC TATAGTGACA 
TTAGTGGAGC TGGGCGATGA CTGGGAGCGA ACTGAGGTCC TGCATAAGAA GTTTGAAGAA 
TTCCAGGAAG AACTGACCGC TCGGAAGGGA AAAGTGGACA GAGTGAACCA ATATGCTAAT 
GAGTGTGCCC AGGAGAAACA TCCTAAGTTA CCAGAGATAA AGGCAAAACA GGATGAAGTC 
AATGCTGCCT GGGATCGTCT CTGGAGCCTG GCTCTCAAGA GACGAGAAAG TCTGTCTAAT 
GCAGCAGACC TCCAGCGATT TAAAAGGGAT GTCAATGAAG CTATCCAGTG GATGGAGGAG 
AAGGAGCCGC AGCTCACCTC TGAGGACTAT GGCAAAGATC TTGTTAGTTC TGAGGCATTG 
TTTCACAATC ACAAGAGGCT CGAGAGGAAT CTCGCGGTCA TGGATGACAA GGTGAAGGAG 
TTGTGTGCCA AAGCAGACAA GCTGATGATT TCCCATTCTG CAGATGCACC TCAGATTCAA 
CAGATGAAAT TGGATCTAGT CTCCAACTGG GAACGCATCC GTGCCTTGGC CACTAACAGA 
TATGCAAAGC TGAAGGCTTC TTATGGGTAC CATCGGTTCT TATCTGACTA TGATGAGCTC 
TCAGGCTGGA TGAAGGAAAA GACTGCTCTG ATCAATGCTG ATGAGCTGCC AACAGATGTG 
GCCAGTGGAG AAGCCCTGCT AGCCAGGCAC CAGCAGCATA AGCATGAGAT TGACTCTTAT 
GATGATCGAT TTCAATCTGC TGATGCAACT GGTCAAGAAC TCCTGGATGG CAATCACGAA 
GCTTCTGAGG AAATTCGAGA GAAGATGACA ATACTTGCCA ATGACTGGGC TGCTCTGCTG 
GAACTGTGGG ACAAGTGTCA GCACCAATAC CGCCAATGTC TGGATTTCCA TCTCTTCTAC 
CGAGACAGTG AACAAGTGGA TAGTTGGATG AGCAGACAAG AGGCTTTCCT GGAAAATGAA 
GACCTGGGGA ACTCAGTGGG CAGCGTGGAA GCCCTCCTTC AGAAGCATGA TGACTTTGAA 
GAAGCGTTCA CTGCCCAGGA AGAAAAGATC ATAACCTTGG ATGAGACTGC AACCAAGCTT 
ATTGACAATG ACCACTATGA CTCTGAGAAT ATCGCTGCTA TTCGTGATGG GCTTTTGGCC 
AGACGTGATG CCCTGCGTGA AAGGGCTGCC ACTAGAAGAA AATTGTTGGT AGATTCACAA 
CTTCTTCAAC AACTGTATCA AGACTCAGAT GACCTAAAAA CCTGGATCAA TAAGAAGAAA 
AAGTTGGCTG ATGATGACGA TTACAAGGAT GTGCAGAACT TGAAGAGCCG GGTTCAGAAG 
CAGCAGGACT TTGAAGAGGA ATTGGCAGTG AATGAGATTA TGCTCAATAA CTTGGAGAAA 
ACAGGCCAGG AGATGATTGA GGATGGTCAC TACGCCTCTG AGGCTGTGGC TGCTCGCCTG 
AGTGAGGTTG CCAACCTCTG GAAGGAGTTG CTGGAGGCAA CAGCACAAAA AGGGACTCAG 
CTGTATGAAG CTAACCAGCT GCTACAGTTT GAGAACAATG CAGAAGACCT GAAGCGCTGG 
CTAGAGGAGG TGGAGTGGCA GGTTACCTCT GAGGATTATG GGAAAGGTCT GGCTGATGTA 
CAGAATCTAC TAAGAAAACA TGGCCTTCTA GAGTCAGATG TGACTGCTCG TCAGAATCAG 
GTGGACACCC TCACAGACAT GGCGGCACAC TTTGAAGAGA TAGGCCATCC CGACTCTGGG 
GACATCCGTG CAAGGCAGGA ATCCTTGCTT TCCCGTTTTG AGGCTCTGAA GGAGCCACTG 
GCCATTCGGA AGAAGAAGCT CATTGACCTA CTCAAGCTAC AGCAGATTTG CAGAGACTCG 
GAAGATGAGG AGGCGTGGAT CCAGGAGACT GAGCCCTCAG CAGCTTCCAC TCATCTTGGA 
AAGGACTTGG TTGCAGCCAA GAATCTTCTA AACAGACATG AAGTCATCCT GGCAGACATT 
GCGAGTCATG AACCACGGAT TCAAGTAATA ACAGAGAGGG GAAACAAAAT GGTGGAGGAA 
GGACACTTCG CTGCAGAAGA CATAGCATCT AGGGTTGAAA GCTTGAACAA AAATATGGAG 
TCTCTCCATG CTCGAGCTAT TAGACGGGAG AATGATCTTA AGGCCAATGT CCAGCTCCAA 
CAGTACCTGG CAGATCTGCA TGAAGCAGAA GCATGGATTA AAGAGAAAGA ACCCATTGTA 
GACAACAAAA ACTATGGAGC TGATGAAGAA GCAGCTGGGG CCCTTCTCAA GAAGCATGAA 
GCCTTCCTGG TGGATCTGAA TGCATTTGAA AACAGCATAA AGGCTCTAAG AGATCAGGCA 
GAAGTCTGCC AGCAACAGCA GGCAGCACCA GTGGACGAGG CTGGTCGAGA AGCTAGGGTC 
ATAGCTTTGT ATGACTTTGA GGCTCGCAGC CGTAGAGAGG TCAGCATGAA GAAGAATGAT 
GTCTTAACTC TGCTCAGTTC CATCAACAAG GACTGGTGGA AGGTTGAAGC TGATGATCAC 
CAGGGCTTTG TGCCAGCGGT TTATGTCAGG AAACTGGCCC CCGATGAGCT ACCAGGTTTC 
CCACAGCATC GGCAAGAGGA GCCAGTCAAC ATCCCTCAGC TCCAGCAGCA GGTCGAGACC 
CTGTACCACT CCCTCCTAGA TCGGGCAGAA GAGCGCAGAC GTCGCTTGCT GCAGCGCTAC 
AATGAGTTTC TGCTGGCCTA TGAGGCAGGA GACATGTTGG AGTGGATCCA AGAGAAAAAG 
ACAGAAAACA CTGGAGTGGA ACTGGATGAT GTTTGGGAAC TGCAGAAGAA GTTTGACGAG 
TTCCAGAGGG ATCTAAAGAG TAATGAGCCT CGGCTAAAGG ACATCAACAA AGTGGCTGAC 
GAACTGCTCT TTGAAGAGCT CCTAACCCCA GAAGGAGCTC ACATTCGACA GGAGTTGAAC 
ACCCGCTGGA ATTCCCTGAA GAGGCTGGCA GATGAACAGT ATCAGCTGCT CAGCAGTGCA 
CATGCTGTTG AGATGTTTCA CAGGGAAGCA GATGATGTCA AGGAACAGAT TGATAAGAAA 
TGCCGGGCTC TCAATGCTGC CGACCCTGGC TCTGACCTGC TCAGTGTCCA GGCCCTCCAG 
AGGCAACATG AAGTCTTTGA GAGAGACATC ATCCCCCTGG GAGAAAAGGT GACTACGCTG 
GGGGAGACTG CTGAGCGTCT CTGTGAATCA CACCCAGATG CCACTGAGGA CCTACAGAAG 
CAGAGAACAG AGCTGAATGA GGCCTGGGAC ACACTTCAAG GTCTTACAAG TGATCGGAAG 
GAGAGTCTGA ATGAAGCCCA TAAATTCTTC CTCTTCCTTA GCAAGGCCAG TGATCTAGAG 
AACTGGATCA AAACCATTGG CGGTGTGATA TCATCACCTG AGCTGGCTGA GGACTTAACT 
GGCACAGAGA TCTTGCTGGA GCGACACCAG GAGCATCACG ATGACATAAA GAGAGAGGAC 
CCTACCTTCC AGGCCTTAGA AGACTTTGGT ACAGAACTTA TAGACAGCGG CCACCGTAAC 
AGACGTGAAA TTGACAATAC TCTCCAGAAT ATTAATTCAA AGAGAGACAA TTTGGAAAAG 
AGTTGGGAAA ACCGCAAGAA GATGCTAGAC CAGTGCTTAG AACTGCAGTT ATTCCGAGGA 
AAGTGTGACC AGGTCGAGAG CTGGATGGTG GCCCGTGAGA ATTCCCTGAG GTCAGACGAC 
AGGGATCACT TAAACAGTCT GCAGGCTTTG ATGAAGAAAC GGGATGATTT GGACAAAGCA 
ATCACTGCCC AGGAAGGGAA GATTTCGGAC CTAGAGAACG TTGCCACAAG GCTCATAGAC 
AATGACCACT ATGCCAAGGA AGAGATTGCT GCTCGCCTCC AACGTGTGCT AGACAGGTGG 
AAGGCTCTCA AAGAACAGCT GCTTACTGAA CTGGGAAAGC TAGGAGACTA TGCTGACCTC 
AAACAGTTCT ATCGTGACCT TGAGGACCTG GAGGAATGGA TCAATGAGAT GCTTCCCATA 
GCCTGTGATG AATCTTATAA AGATCCCACT AACATTCAGA GGAAATATCT GAAGCATCAA 
GCCTTTGAAA ATGAAGTCAA CGGTCGTGCT GAGCAGGTGG ATGGGGTTAT CAACCTTGGG 
AATTCCCTGA TTGAGCGCAG AGTGTGTGAT GGAGATGAAG AGAACATGCA GGAGCAACTG 
GACAAGCTAA AGGAGAACTG GGATTATCTC CTAGAGAGAA CCACTGACAA AGGACAGAAG 
CTCAACGAGG CCAGTCGGCA GCAAAGGTTC AACACAAGCA TCCGGGACTT TGAGTTCTGG 
CTTTCAGAGG CAGAAGGCTT GTTGGCCATG AAGGATCAGG CCAGGGACTT AACTTCAGCA 
GGAAACCTAC TTAAGAAGCA TCAGCTATTG GAAGCAGAGA TGTTGGCACG AGAGGACCCT 
CTCAAAGATC TGAATGACCT GGCTCAGGAG CTGATCTCCA GTGGGACTTT CAACATTGAC 
CAGATAGAAG AGAAAATGAA TGGTGTCAAT GAGCGCTTTG AAAATGTCCA GAGCTTGGCA 
GCTGCACACC ACGAGAAGCT GAAAGAGACG TATGCCTTGT TCCAGTTCTT CCAAGACCTG 
GATGATGAGG AAGCCTGGAT AGAGGAGAAG TTGTTACGAG TGAGCTCCCA GGACTATGGG 
AGGGATCTTC AGAGTGTTCA GAACTTATTG AAAAAACACA AACGCCTAGA GGGGGAGCTA 
GTGGCACATG AACCTGCTGT CCAGAATGTG CTGGATACGG CAGAAAGCCT GAGAGACAAG 
GCTGCTGTAG GGAAAGAGGA GATCCAGGAG CGGCTGGCTC AGTTTGTCCA ACACTGGGAA 
AAGCTCAAAG AGTTGGCGAA GACCCGAGGG GTGAACTTGG AGGAGTCCCT CGAGTATCTG 
CAGTTCATGG AGAATGCAGA AGAAGAGGAA GCATGGCTGG GGGAGAAGTG TGCTCTGGTC 
AGTCGCGGGG ATTCAGGAGA CACACTGGCT GCTACTCAGA GTTTGCTAAA GAAGCATGAA 
GCTTTGGAGA ATGACTTTGC TGTGCACAAG AACCGGGTAC AAGATGTTTG TGCACAAGGA 
GAAGACATTC TTAATAAGGA AGAAACTCAG AACAAGGATA AGATTTCCAC CAAGATCCAA 
GTTCTGAATG AAAAGACAGC CTCTTTGGCC AAGGCATTAG CTGCTTGGAA GTCACAACTG 
GACGATGTGC ATGCCTTTCA GCAGTTTAAC TGGAAGGCGG ACGTAGTGGA ATCCTGGATA 
GGTGAGAAAG AAGCAAGCCT GAAGACCAAG AGCAACGGTG CAGACCTCAC CGCCTTCCTC 
ACACTACTGG CAAAGCACGA CACACTGGAT GCCAGTCTTC AGAGCTTCCA GCAAGAAAGG 
CTTTCTGAAA TCGCTGAGCT GAAGGACCAG CTTGTGGCTG GTGAGCACAG CCAAGCCAAA 
GCCATTGAGG AGCAGCATGC AGCCTTGCTG AGGCACTGGG AACAACTGCT GGAGGCATCT 
AGAGTTCACA GGCAGAAACT GCTGGAAAAG CAGCTGCCTC TACAGAAGGC TGAGGAGCTA 
TTCATGGAAT TTGCACACAA AGCTTCAGCC TTCAACAACT GGTGTGAGAA CGCGGAAGAG 
GACTTGTCAG AACCTGTGCA TTGTGTCTCT CTAAATGAGA TCCGGCAGCT GCAGAAGGAA 
CATGAAGCCT TCTTGGCCTC TCTGGCTGGG GCTCAAGAGG ATTTCAACTA TTTGTTGGAG 
CTAGACAAGC AAATTAAAGC CTTAAATGTC CCCTCCAGTC CTTATACCTG GTTAACAGTG 
GATGTGCTGG GCAGAATCTG GAACCACCTG CCAGACATCA TCAAGGAAAG GGAACAGGAA 
CTACAGAAAG AAGAGGCTAG GCAGATCAAG AACTTTGAAA TGTGCCAGGA GTTTGAACAA 
AATGCCAGTG CCTTCCTTCA GTGGATCCAG GAGACCAGGG CCTATTTTCT GGATGGATCA 
TTGCTCAAGG AAACAGGAAC TCTGGAATCC CAACTTGAAG CAAATAAAAG GAAACAGAAG 
GAGATCCAGG CAATGAAGCG CCATCTGACC AAGATTGAAG ACCTGGGGGA CAGCATGGAA 
GAGGCTCTGA TCCTTGATAT CAAATACAGC ACCATTGGAC TGGCCCAGCA ATGGGATCAG 
CTGCACCAAC TAGGGATGCG AATGCAACAC AATTTGGAGC AACAGATTCA AGCCAAAGAC 
ACCATAGGTG TGAGTGAGGA GACATTGAAA GAATTTAGCA CCACATACAA ACACTTCGAT 
GAGAACTTGA CAGGGCGATT GACTCACAAA GAGTTCCGCT CTTGCCTGAG AGGACTCAAT 
TACTATCTGC CTATGGTAGA GGAAGGTGAA CCTGAGCCCA AGTTTGAGAA ATTCCTGAAT 
GCTGTGGATC CAGGGAGGAA AGGATATGTT TCACTGGAAG ACTATACATC ATTCCTGATT 
GACAAGGAGT CAGAGAATAT CAAGACCAGC GATGACATAG AGAGTGCATT CCAAGCTCTG 
GCAGAAGGCA AGGCCTATAT TACCAAGGAG GACATGAAAC AGGCTCTCAC CCCGGAACAA 
GTGTCATTCT GTACCATACA TATGCAGCAA TATATGGATC CACGGGGTCG GAGCCAACCT 
GCTGGCTACG ACTATGTTGG CTTCACCAAT TCATTCTTTG GCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Scientific reports7(1)5164(2017 Jul)
Huang YS,Delgadillo LF,Cyr KH,Kingsley PD,An X,McGrath KE,Mohandas N,Conboy JG,Waugh RE,Wan J,Palis J

A postprandial FGF19-SHP-LSD1 regulatory axis?mediates epigenetic repression of hepatic?autophagy.
The EMBO journal36(12)1755-1769(2017 06)
Byun S,Kim YC,Zhang Y,Kong B,Guo G,Sadoshima J,Ma J,Kemper B,Kemper JK

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.
Scientific reports637197(2016 Nov)
Huang HM,Bauer DC,Lelliott PM,Greth A,McMorran BJ,Foote SJ,Burgio G

?II-spectrin regulates invadosome stability and extracellular matrix degradation.
PloS one10(4)e0120781(2015)
Ponceau A,Albig?s-Rizo C,Colin-Aronovicz Y,Destaing O,Lecomte MC

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Spta1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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