Spn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spn
Entrez Gene ID	20737
Full Name	sialophorin
Synonyms	A630014B01Rik,Cd43,Galgp,Ly-48,Ly48
General protein information	Preferred Names sialophorin Names leukosialin 3E8 antigen B-cell differentiation antigen LP-3 leukocyte sialoglycoprotein lymphocyte antigen 48
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 F3\|7 69.35 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006507522.3	XP_006507585.1	leukosialin isoform X2
XM_006507522.4	XP_006507585.1	leukosialin isoform X2
NM_001037810.2	NP_001032899.1	leukosialin precursor
XM_011241717.2	XP_011240019.1	leukosialin isoform X1
NM_009259.5	NP_033285.1	leukosialin precursor
XM_011241716.2	XP_011240018.1	leukosialin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPN	XP_523332.2
Rattus norvegicus (Norway rat)	Spn	NP_001258015.1
Homo sapiens (human)	SPN	NP_001025459.1
Canis lupus familiaris (dog)	SPN	XP_005621391.1
Bos taurus (cattle)	SPN	NP_001096572.1
Mus musculus (house mouse)	Spn	NP_001032899.1

Gene Ontology
Bibliography

Related articles in PubMed

Co-infection with Chikungunya virus alters trafficking of pathogenic CD8
Teo TH, Howland SW, Claser C, Gun SY, Poh CM, Lee WW, Lum FM, Ng LF, R?nia L
EMBO molecular medicine10(1)121-138(2018 Jan)

CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo.
Vel?zquez F, Grodecki-Pena A, Knapp A, Salvador AM, Nevers T, Croce K, Alcaide P
Journal of immunology (Baltimore, Md. : 1950)196(3)1305-1316(2016 Feb)

Glycosylation Status of CD43 Protein Is Associated with Resistance of Leukemia Cells to CTL-Mediated Cytolysis.
Hasegawa K, Tanaka S, Fujiki F, Morimoto S, Nakano K, Kinoshita H, Okumura A, Fujioka Y, Urakawa R, Nakajima H, Tatsumi N, Nakata J, Takashima S, Nishida S, Tsuboi A, Oka Y, Oji Y, Miyoshi E, Hirata T, Kumanogoh A, Sugiyama H, Hosen N
PloS one11(3)e0152326(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings demonstrate that CD43 is a major E-selectin ligand in Th17 cells that functions independent of P-selectin glycoprotein ligand-1
Title: CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo.

glycosylation status of CD43 protein on leukemia cells is associated with sensitivity to CTL-mediated cytolysis
Title: Glycosylation Status of CD43 Protein Is Associated with Resistance of Leukemia Cells to CTL-Mediated Cytolysis.

CD43 may be an important mediator of macrophage lipid homeostasis, thereby affecting macrophage foam cell formation and ultimately atherosclerotic plaque development.
Title: Cluster of differentiation 43 deficiency in leukocytes leads to reduced atherosclerosis--brief report.

We have identified a robust physiologic role for CD43 in a relevant animal model and established an important in vivo function for CD43-dependent regulation of IFN-gamma production.
Title: CD43-mediated IFN-? production by CD8+ T cells promotes abdominal aortic aneurysm in mice.

Results show that while CD43 binding to ezrin-radixin-moesin proteins is crucial for S76 phosphorylation, CD43 movement and regulation of T-cell migration can occur through an ERM-independent, phosphorylation-dependent mechanism.
Title: CD43 interaction with ezrin-radixin-moesin (ERM) proteins regulates T-cell trafficking and CD43 phosphorylation.

The following Spn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00031	XM_006507522.4 Latest version!	Mus musculus sialophorin (Spn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu00031	NM_001037810.2 Latest version!	Mus musculus sialophorin (Spn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu00031	NM_009259.5 Latest version!	Mus musculus sialophorin (Spn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu68778	XM_011241717.2 Latest version!	Mus musculus sialophorin (Spn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu68778	XM_011241716.2 Latest version!	Mus musculus sialophorin (Spn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OMu00031	XM_006507522.3	Mus musculus sialophorin (Spn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spn ( XM_006507522.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507585.1 Mus musculus sialophorin (Spn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu00031
Clone ID Related Accession (Same CDS sequence)	XM_006507522.3 , XM_006507522.4 , NM_001037810.2 , NM_009259.5
Accession Version	XM_006507522.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leukosialin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507522.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507585.1
CDS	1324..2511
Translation	MALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL

Target ORF information:

RefSeq Version	XM_006507522.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialophorin (Spn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507522.4

ORF Insert Sequence:

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spn ( NM_001037810.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001032899.1 Mus musculus sialophorin (Spn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00031
Clone ID Related Accession (Same CDS sequence)	XM_006507522.3 , XM_006507522.4 , NM_001037810.2 , NM_009259.5
Accession Version	NM_001037810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	leukosialin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122537.4, AK134098.1 and AK041712.1. On Jan 18, 2014 this sequence version replaced NM_001037810.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK041712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032899.1
CDS	112..1299
Translation	MALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL

Target ORF information:

RefSeq Version	NM_001037810.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialophorin (Spn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037810.2

ORF Insert Sequence:

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spn ( NM_009259.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033285.1 Mus musculus sialophorin (Spn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00031
Clone ID Related Accession (Same CDS sequence)	XM_006507522.3 , XM_006507522.4 , NM_001037810.2 , NM_009259.5
Accession Version	NM_009259.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	leukosialin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122537.4, AK134098.1, AK077764.1 and AW540100.2. On Jan 18, 2014 this sequence version replaced NM_009259.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077764.1, AK134098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033285.1
CDS	112..1299
Translation	MALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL

Target ORF information:

RefSeq Version	NM_009259.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialophorin (Spn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009259.5

ORF Insert Sequence:

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spn ( XM_006507522.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507585.1 Mus musculus sialophorin (Spn), transcript variant X4, mRNA.

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CloneID	OMu00031
Clone ID Related Accession (Same CDS sequence)	XM_006507522.3 , XM_006507522.4 , NM_001037810.2 , NM_009259.5
Accession Version	XM_006507522.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	leukosialin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507585.1
CDS	254..1441
Translation	MALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL

Target ORF information:

RefSeq Version	XM_006507522.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialophorin (Spn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507522.3

ORF Insert Sequence:

ATGGCCTTGC ACCTTCTCCT CCTCTTTGGG GCATGCTGGG TCCAGGTGGC GAGCCCAGAC 
AGTCTGCAGA GGACGACGAT GCTACCATCT ACCCCACATA TCACAGCTCC AAGTACCTCT 
GAAGCCCAGA ATGCCAGCCC ATCAGTTTCT GTGGGGTCAG GGACAGTGGA CTCGAAGGAG 
ACCATCAGCC CCTGGGGGCA GACCACAATC CCAGTTTCTT TGACCCCCTT GGAAACTACT 
GAATTGTCTT CTTTGGAGAC TTCTGCTGGT GCCAGCATGA GCACCCCTGT ACCTGAGCCT 
ACTGCCTCTC AGGAAGTTTC CAGCAAGACA TCAGCACTGC TTCCAGAACC ATCAAATGTA 
GCCAGTGACC CTCCTGTCAC TGCAGCCAAT CCTGTGACAG ACGGACCTGC AGCCAATCCT 
GTGACAGACG GAACTGCAGC ATCTACATCT ATCTCTAAAG GAACCAGTGC ACCCCCCACC 
ACTGTGACAA CAAGCTCTAA CGAGACCAGT GGACCCTCTG TGGCTACAAC AGTAAGCTCC 
AAGACCAGTG GTCCCCCTGT CACCACAGCT ACTGGGTCTC TGGGGCCCTC GAGTGAGATG 
CATGGACTCC CTGCTACCAC AGCAACCAGT TCTGTGGAGA GTTCCAGTGT GGCCCGTGGC 
ACCTCAGTTT CCAGCAGAAA AACATCCACG ACGTCTACCC AAGATCCCAT AACCACCAGG 
TCACCAAGCC AAGAATCAAG TGGCATGTTA CTGGTGCCCA TGCTTATTGC CTTGGTGGTG 
GTTTTGGCCC TCGTGGCGCT ACTGCTGTTG TGGCGCCAGA GGCAAAAGCG GAGGACTGGG 
GCCCTGACCC TGAGCGGAGG AGGAAAACGA AATGGGGTGG TGGATGCCTG GGCTGGGCCA 
GCCCGGGTTC CTGATGAAGA GGCCACAACC ACATCAGGGG CAGGGGGCAA CAAGGGCTCT 
GAGGTACTGG AGACAGAAGG CTCCGGGCAG AGACCCACGC TCACTACGTT CTTCAGCAGA 
CGGAAGTCTC GCCAGGGCTC TTTAGTACTA GAAGAGCTGA AGCCTGGGTC GGGTCCCAAC 
CTGAAGGGGG AGGAAGAGCC GCTTGTGGGC AGTGAGGATG AAGCTGTGGA AACCCCAACT 
TCTGACGGTC CACAAGCCAA AGATGAGGCC GCACCTCAAT CTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spn ( XM_011241717.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240019.1 Mus musculus sialophorin (Spn), transcript variant X2, mRNA.

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CloneID	OMu68778
Clone ID Related Accession (Same CDS sequence)	XM_011241717.2 , XM_011241716.2
Accession Version	XM_011241717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leukosialin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAA GGTCCCAACT CCTGCTCCTG CCTGTCCCTC TGGAGATGGC CTTGCACCTT 
CTCCTCCTCT TTGGGGCATG CTGGGTCCAG GTGGCGAGCC CAGACAGTCT GCAGAGGACG 
ACGATGCTAC CATCTACCCC ACATATCACA GCTCCAAGTA CCTCTGAAGC CCAGAATGCC 
AGCCCATCAG TTTCTGTGGG GTCAGGGACA GTGGACTCGA AGGAGACCAT CAGCCCCTGG 
GGGCAGACCA CAATCCCAGT TTCTTTGACC CCCTTGGAAA CTACTGAATT GTCTTCTTTG 
GAGACTTCTG CTGGTGCCAG CATGAGCACC CCTGTACCTG AGCCTACTGC CTCTCAGGAA 
GTTTCCAGCA AGACATCAGC ACTGCTTCCA GAACCATCAA ATGTAGCCAG TGACCCTCCT 
GTCACTGCAG CCAATCCTGT GACAGACGGA CCTGCAGCCA ATCCTGTGAC AGACGGAACT 
GCAGCATCTA CATCTATCTC TAAAGGAACC AGTGCACCCC CCACCACTGT GACAACAAGC 
TCTAACGAGA CCAGTGGACC CTCTGTGGCT ACAACAGTAA GCTCCAAGAC CAGTGGTCCC 
CCTGTCACCA CAGCTACTGG GTCTCTGGGG CCCTCGAGTG AGATGCATGG ACTCCCTGCT 
ACCACAGCAA CCAGTTCTGT GGAGAGTTCC AGTGTGGCCC GTGGCACCTC AGTTTCCAGC 
AGAAAAACAT CCACGACGTC TACCCAAGAT CCCATAACCA CCAGGTCACC AAGCCAAGAA 
TCAAGTGGCA TGTTACTGGT GCCCATGCTT ATTGCCTTGG TGGTGGTTTT GGCCCTCGTG 
GCGCTACTGC TGTTGTGGCG CCAGAGGCAA AAGCGGAGGA CTGGGGCCCT GACCCTGAGC 
GGAGGAGGAA AACGAAATGG GGTGGTGGAT GCCTGGGCTG GGCCAGCCCG GGTTCCTGAT 
GAAGAGGCCA CAACCACATC AGGGGCAGGG GGCAACAAGG GCTCTGAGGT ACTGGAGACA 
GAAGGCTCCG GGCAGAGACC CACGCTCACT ACGTTCTTCA GCAGACGGAA GTCTCGCCAG 
GGCTCTTTAG TACTAGAAGA GCTGAAGCCT GGGTCGGGTC CCAACCTGAA GGGGGAGGAA 
GAGCCGCTTG TGGGCAGTGA GGATGAAGCT GTGGAAACCC CAACTTCTGA CGGTCCACAA 
GCCAAAGATG AGGCCGCACC TCAATCTCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240019.1
CDS	285..1517
Translation	MDSRSQLLLLPVPLEMALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL

Target ORF information:

RefSeq Version	XM_011241717.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialophorin (Spn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241717.2

ORF Insert Sequence:

ATGGATTCAA GGTCCCAACT CCTGCTCCTG CCTGTCCCTC TGGAGATGGC CTTGCACCTT 
CTCCTCCTCT TTGGGGCATG CTGGGTCCAG GTGGCGAGCC CAGACAGTCT GCAGAGGACG 
ACGATGCTAC CATCTACCCC ACATATCACA GCTCCAAGTA CCTCTGAAGC CCAGAATGCC 
AGCCCATCAG TTTCTGTGGG GTCAGGGACA GTGGACTCGA AGGAGACCAT CAGCCCCTGG 
GGGCAGACCA CAATCCCAGT TTCTTTGACC CCCTTGGAAA CTACTGAATT GTCTTCTTTG 
GAGACTTCTG CTGGTGCCAG CATGAGCACC CCTGTACCTG AGCCTACTGC CTCTCAGGAA 
GTTTCCAGCA AGACATCAGC ACTGCTTCCA GAACCATCAA ATGTAGCCAG TGACCCTCCT 
GTCACTGCAG CCAATCCTGT GACAGACGGA CCTGCAGCCA ATCCTGTGAC AGACGGAACT 
GCAGCATCTA CATCTATCTC TAAAGGAACC AGTGCACCCC CCACCACTGT GACAACAAGC 
TCTAACGAGA CCAGTGGACC CTCTGTGGCT ACAACAGTAA GCTCCAAGAC CAGTGGTCCC 
CCTGTCACCA CAGCTACTGG GTCTCTGGGG CCCTCGAGTG AGATGCATGG ACTCCCTGCT 
ACCACAGCAA CCAGTTCTGT GGAGAGTTCC AGTGTGGCCC GTGGCACCTC AGTTTCCAGC 
AGAAAAACAT CCACGACGTC TACCCAAGAT CCCATAACCA CCAGGTCACC AAGCCAAGAA 
TCAAGTGGCA TGTTACTGGT GCCCATGCTT ATTGCCTTGG TGGTGGTTTT GGCCCTCGTG 
GCGCTACTGC TGTTGTGGCG CCAGAGGCAA AAGCGGAGGA CTGGGGCCCT GACCCTGAGC 
GGAGGAGGAA AACGAAATGG GGTGGTGGAT GCCTGGGCTG GGCCAGCCCG GGTTCCTGAT 
GAAGAGGCCA CAACCACATC AGGGGCAGGG GGCAACAAGG GCTCTGAGGT ACTGGAGACA 
GAAGGCTCCG GGCAGAGACC CACGCTCACT ACGTTCTTCA GCAGACGGAA GTCTCGCCAG 
GGCTCTTTAG TACTAGAAGA GCTGAAGCCT GGGTCGGGTC CCAACCTGAA GGGGGAGGAA 
GAGCCGCTTG TGGGCAGTGA GGATGAAGCT GTGGAAACCC CAACTTCTGA CGGTCCACAA 
GCCAAAGATG AGGCCGCACC TCAATCTCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spn ( XM_011241716.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240018.1 Mus musculus sialophorin (Spn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68778
Clone ID Related Accession (Same CDS sequence)	XM_011241717.2 , XM_011241716.2
Accession Version	XM_011241716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leukosialin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAA GGTCCCAACT CCTGCTCCTG CCTGTCCCTC TGGAGATGGC CTTGCACCTT 
CTCCTCCTCT TTGGGGCATG CTGGGTCCAG GTGGCGAGCC CAGACAGTCT GCAGAGGACG 
ACGATGCTAC CATCTACCCC ACATATCACA GCTCCAAGTA CCTCTGAAGC CCAGAATGCC 
AGCCCATCAG TTTCTGTGGG GTCAGGGACA GTGGACTCGA AGGAGACCAT CAGCCCCTGG 
GGGCAGACCA CAATCCCAGT TTCTTTGACC CCCTTGGAAA CTACTGAATT GTCTTCTTTG 
GAGACTTCTG CTGGTGCCAG CATGAGCACC CCTGTACCTG AGCCTACTGC CTCTCAGGAA 
GTTTCCAGCA AGACATCAGC ACTGCTTCCA GAACCATCAA ATGTAGCCAG TGACCCTCCT 
GTCACTGCAG CCAATCCTGT GACAGACGGA CCTGCAGCCA ATCCTGTGAC AGACGGAACT 
GCAGCATCTA CATCTATCTC TAAAGGAACC AGTGCACCCC CCACCACTGT GACAACAAGC 
TCTAACGAGA CCAGTGGACC CTCTGTGGCT ACAACAGTAA GCTCCAAGAC CAGTGGTCCC 
CCTGTCACCA CAGCTACTGG GTCTCTGGGG CCCTCGAGTG AGATGCATGG ACTCCCTGCT 
ACCACAGCAA CCAGTTCTGT GGAGAGTTCC AGTGTGGCCC GTGGCACCTC AGTTTCCAGC 
AGAAAAACAT CCACGACGTC TACCCAAGAT CCCATAACCA CCAGGTCACC AAGCCAAGAA 
TCAAGTGGCA TGTTACTGGT GCCCATGCTT ATTGCCTTGG TGGTGGTTTT GGCCCTCGTG 
GCGCTACTGC TGTTGTGGCG CCAGAGGCAA AAGCGGAGGA CTGGGGCCCT GACCCTGAGC 
GGAGGAGGAA AACGAAATGG GGTGGTGGAT GCCTGGGCTG GGCCAGCCCG GGTTCCTGAT 
GAAGAGGCCA CAACCACATC AGGGGCAGGG GGCAACAAGG GCTCTGAGGT ACTGGAGACA 
GAAGGCTCCG GGCAGAGACC CACGCTCACT ACGTTCTTCA GCAGACGGAA GTCTCGCCAG 
GGCTCTTTAG TACTAGAAGA GCTGAAGCCT GGGTCGGGTC CCAACCTGAA GGGGGAGGAA 
GAGCCGCTTG TGGGCAGTGA GGATGAAGCT GTGGAAACCC CAACTTCTGA CGGTCCACAA 
GCCAAAGATG AGGCCGCACC TCAATCTCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240018.1
CDS	285..1517
Translation	MDSRSQLLLLPVPLEMALHLLLLFGACWVQVASPDSLQRTTMLPSTPHITAPSTSEAQNASPSVSVGSGTVDSKETISPWGQTTIPVSLTPLETTELSSLETSAGASMSTPVPEPTASQEVSSKTSALLPEPSNVASDPPVTAANPVTDGPAANPVTDGTAASTSISKGTSAPPTTVTTSSNETSGPSVATTVSSKTSGPPVTTATGSLGPSSEMHGLPATTATSSVESSSVARGTSVSSRKTSTTSTQDPITTRSPSQESSGMLLVPMLIALVVVLALVALLLLWRQRQKRRTGALTLSGGGKRNGVVDAWAGPARVPDEEATTTSGAGGNKGSEVLETEGSGQRPTLTTFFSRRKSRQGSLVLEELKPGSGPNLKGEEEPLVGSEDEAVETPTSDGPQAKDEAAPQSL

Target ORF information:

RefSeq Version	XM_011241716.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialophorin (Spn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241716.2

ORF Insert Sequence:

ATGGATTCAA GGTCCCAACT CCTGCTCCTG CCTGTCCCTC TGGAGATGGC CTTGCACCTT 
CTCCTCCTCT TTGGGGCATG CTGGGTCCAG GTGGCGAGCC CAGACAGTCT GCAGAGGACG 
ACGATGCTAC CATCTACCCC ACATATCACA GCTCCAAGTA CCTCTGAAGC CCAGAATGCC 
AGCCCATCAG TTTCTGTGGG GTCAGGGACA GTGGACTCGA AGGAGACCAT CAGCCCCTGG 
GGGCAGACCA CAATCCCAGT TTCTTTGACC CCCTTGGAAA CTACTGAATT GTCTTCTTTG 
GAGACTTCTG CTGGTGCCAG CATGAGCACC CCTGTACCTG AGCCTACTGC CTCTCAGGAA 
GTTTCCAGCA AGACATCAGC ACTGCTTCCA GAACCATCAA ATGTAGCCAG TGACCCTCCT 
GTCACTGCAG CCAATCCTGT GACAGACGGA CCTGCAGCCA ATCCTGTGAC AGACGGAACT 
GCAGCATCTA CATCTATCTC TAAAGGAACC AGTGCACCCC CCACCACTGT GACAACAAGC 
TCTAACGAGA CCAGTGGACC CTCTGTGGCT ACAACAGTAA GCTCCAAGAC CAGTGGTCCC 
CCTGTCACCA CAGCTACTGG GTCTCTGGGG CCCTCGAGTG AGATGCATGG ACTCCCTGCT 
ACCACAGCAA CCAGTTCTGT GGAGAGTTCC AGTGTGGCCC GTGGCACCTC AGTTTCCAGC 
AGAAAAACAT CCACGACGTC TACCCAAGAT CCCATAACCA CCAGGTCACC AAGCCAAGAA 
TCAAGTGGCA TGTTACTGGT GCCCATGCTT ATTGCCTTGG TGGTGGTTTT GGCCCTCGTG 
GCGCTACTGC TGTTGTGGCG CCAGAGGCAA AAGCGGAGGA CTGGGGCCCT GACCCTGAGC 
GGAGGAGGAA AACGAAATGG GGTGGTGGAT GCCTGGGCTG GGCCAGCCCG GGTTCCTGAT 
GAAGAGGCCA CAACCACATC AGGGGCAGGG GGCAACAAGG GCTCTGAGGT ACTGGAGACA 
GAAGGCTCCG GGCAGAGACC CACGCTCACT ACGTTCTTCA GCAGACGGAA GTCTCGCCAG 
GGCTCTTTAG TACTAGAAGA GCTGAAGCCT GGGTCGGGTC CCAACCTGAA GGGGGAGGAA 
GAGCCGCTTG TGGGCAGTGA GGATGAAGCT GTGGAAACCC CAACTTCTGA CGGTCCACAA 
GCCAAAGATG AGGCCGCACC TCAATCTCTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Co-infection with Chikungunya virus alters trafficking of pathogenic CD8
EMBO molecular medicine10(1)121-138(2018 Jan)
Teo TH,Howland SW,Claser C,Gun SY,Poh CM,Lee WW,Lum FM,Ng LF,R?nia L

CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo.
Journal of immunology (Baltimore, Md. : 1950)196(3)1305-1316(2016 Feb)
Vel?zquez F,Grodecki-Pena A,Knapp A,Salvador AM,Nevers T,Croce K,Alcaide P

Glycosylation Status of CD43 Protein Is Associated with Resistance of Leukemia Cells to CTL-Mediated Cytolysis.
PloS one11(3)e0152326(2016)
Hasegawa K,Tanaka S,Fujiki F,Morimoto S,Nakano K,Kinoshita H,Okumura A,Fujioka Y,Urakawa R,Nakajima H,Tatsumi N,Nakata J,Takashima S,Nishida S,Tsuboi A,Oka Y,Oji Y,Miyoshi E,Hirata T,Kumanogoh A,Sugiyama H,Hosen N

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Spn cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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