Hectd1 cDNA ORF clone, Mus musculus(house mouse)

The following Hectd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hectd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu72600 XM_011244049.3
Latest version!
Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu72601 XM_011244050.3
Latest version!
Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu72602 XM_011244051.3
Latest version!
Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu78179 XM_017315008.2
Latest version!
Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu11077 NM_144788.2
Latest version!
Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OMu72601 XM_011244050.2 Mus musculus HECT domain containing 1 (Hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu72600 XM_011244049.2 Mus musculus HECT domain containing 1 (Hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu78179 XM_017315008.1 Mus musculus HECT domain containing 1 (Hectd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu72602 XM_011244051.2 Mus musculus HECT domain containing 1 (Hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu72600
    Clone ID Related Accession (Same CDS sequence) XM_011244049.3 , XM_011244049.2
    Accession Version XM_011244049.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGACCAGCA
    GTTGCATTAA TTCGAAAATT AATAGCTGTA CTAGAATCAA TTGAACGCCT ACCTCTCCAT
    TTGTATGACA CACCAGGATC CACGTATAAC CTCCAGATAC TTACAAGAAG ATTACGCTTT
    CGGTTGGAAC GTGCACCTGG TGAAACTTCA TTAATAGACC GGACAGGCAG GATGCTGAAG
    ATGGAGCCCT TAGCTACAGT TGAATCTCTG GAACAGTATC TGCTGAAAAT GGTGGCAAAG
    CAGTGGTACG ACTTTGACAG ATCCTCATTT GTTTTTGTTC GAAAATTAAG AGAAGGCCAA
    AATTTTATAT TCCGGCACCA ACATGACTTT GATGAGAATG GAATCATTTA CTGGATTGGA
    ACAAATGCAA AAACTGCGTA TGAATGGGTA AATCCTGCAG CTTATGGACT CGTAGTCGTC
    ACATCATCAG AAGGAAGGAA TCTGCCCTAT GGCCGCTTGG AGGACATACT AAGTCGAGAT
    AATTCAGCTT TAAATTGTCA CAGCAATGAT GATAAAAATG CCTGGTTTGC CATAGATCTG
    GGTGTCTGGG TAATACCTTC AGCATACACA CTTCGTCATG CTCGTGGCTA TGGAAGGTCT
    GCACTGAGAA ATTGGGTTTT CCAGGTATCC AAAGATGGAC AGAACTGGAC TTCTTTGTAT
    ACCCATGTTG ATGACTGCAG TCTCAATGAA CCAGGGTCAA CTGCAACATG GCCCCTTGAC
    CCAGCGAAGG ATGAAAAGCA AGGGTGGCGG CATGTCAGGC TGAAGCAGAT GGGGAAGAAC
    GCCAGTGGAC AGACGCACTA CCTCTCGCTC TCCGGCTTTG AACTCTATGG CACTGTGAAT
    GGCGTGTGCG AAGACCAACT AGGGAAAGCA GCTAAAGAAG CAGAAGCTAA TCTTAGAAGG
    CAGAGGCGCC TGGTGCGCTC TCAGGTCCTC AAGTACATGG TTCCAGGAGC ACGTGTTATC
    AGGGGCCTTG ATTGGAAGTG GCGAGATCAG GATGGCAGCC CGCAGGGAGA AGGCACAGTC
    ACAGGCGAGC TCCACAATGG CTGGATTGAT GTCACCTGGG ATGCTGGTGG CTCAAACTCT
    TACCGTATGG GCGCAGAAGG AAAATTTGAC CTCAAGCTTG CACCAGGGTA CGACCCTGAT
    ACAGTGGCAT CACCCAAACC TGTTTCATCC ACTGTTTCAG GCACAACGCA ATCATGGAGC
    AGCTTGGTGA AAAACAACTG TCCAGACAAG ACGTCTGCTG CTGCAGGCTC CTCAAGTAGA
    AAAGGGAGCA GCAGCTCTGT GTGTAGCGTG GCCAGTAGCA GCGACATCAG CTTGGCTTCG
    ACCAAAACGG AACGGAGATC AGAAATTGTA ATGGAACACA GTATAGTTTC AGGAGCTGAT
    GTCCATGAAC CAATTGTTGT TCTTTCATCT GCTGAAAACG TCCCTCAAAC AGAAGTAGGG
    TCATCTTCCA GTGCAAGCAC CAGCACCCTA ACAGCGGAAA CGGGAAGTGA AAATGCGGAA
    AGGAAGTTAG GCCCTGATAG TTCTGTTCGA GCTCCTGGGG AGTCTAGTGC GATATCCATG
    GGGATTGTTA GCGTTAGCTC TCCTGATGTT AGTTCGGTAT CTGAATTAAC CAATAAGGAA
    GCAGCTTCAC AACGACCCCT TAGCTCTTCG GCAAGTAACA GACTGTCGGT GAGCTCTCTG
    TTGGCAGCCG GGGCCCCTAT GAGCTCCAGT GCGAGTGTAC CTAACCTGTC CTCAAGAGAG
    ACATCTAGCT TGGAGAGCTT TGTCAGGAGA GTGGCAAACA TAGCACGGAC TAATGCCACC
    AACAACATGA ACCTAAGCCG AAGCAGCAGT GACAACAACA CTAACACTTT GGGGAGGAAC
    GTGATGAGCA CCGCAACTTC TCCACTTATG GGTGCTCAGA GTTTCCCTAA TTTGACCACA
    CCTGGTACTA CATCAACAGT GACAATGTCA ACATCGAGTG TTACGAGCAG CAGCAATGTA
    GCTACGGCAA CCACAGTTTT ATCAGTGGGT CAATCATTAA GTAATACTTT AACCACCAGC
    CTCACATCAA CCTCTAGTGA GAGTGACACA GGCCAGGAAG CAGAATACTC CTTATATGAT
    TTCCTCGACA GCTGCCGTGC CAGTACTCTC TTAGCTGAAC TAGATGATGA CGAGGATTTG
    CCTGAGCCTG ACGAGGAAGA CGATGAGAAT GAAGATGACA ATCAGGAGGA CCAAGAATAC
    GAGGAGGTTA TGATTCTGAG ACGTCCCTCC CTGCAACGCC GAGCTGGCTC TCGCTCTGAC
    GTAACACACC ATGTCGTCAC CTCCCAGCTC CCACAGGTCC CTTCTGGAGC AGGGAGCCGG
    CCTGTTGGGG AACAGGAAGA AGAAGAGTAT GAAACCAAAG GAGGCCGCCG CAGAGCCTGG
    GATGACGACT ATGTGCTAAA GCGCCAGTTT TCTGCACTGG TCCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CCAATGTCCA GCAGACAACT GACCTAGAAA TTCCTCCCCC AGGAACACCT
    CACTCAGAGC TCTTGGAGGA AGTTGAATGT ACTCCGTCAC CTCGCTTGGC TCTCACACTG
    AAAGTGACGG GGCTTGGAAC AACGCGGGAA GTTGAACTGC CACTTACCAA TTTCAGATCC
    ACCATCTTTT ACTATGTACA AAAACTGCTT CAACTGTCTT GTAATGGCAA TGTGAAGTCA
    GATAAACTTA GGCGTATTTG GGAGCCCACT TACACAATCA TGTATAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGGAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAACAG
    TACCTTGGCA CTGACGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATGCCGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTTAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGATTAAACC AGGGGGCTAT TTCTAGTCTT CAAAGTAGTG ATATTCTTAA TTTGACAAAA
    GAGCAGCCTC AGGCTAAAGC AGGCAACGGG CAGAGCCCCT GTGGAGTAGA GGACGTCCTT
    CAGCTTCTGC GGATTCTGTA TATAGTTGCA AGTGATCCTT ATTCAAGGAT ATCCCAAGAA
    GATGGCGATG AGCAGCCTCA GTTTACTTTT CCACCTGATG AATTTACAAG TAAAAAAATC
    ACCACAAAAA TTTTGCAGCA GATTGAGGAA CCGTTGGCAC TGGCCAGTGG GGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTGA TCCCATTCGA GACGAGGCAG
    CTTTATTTCA CCTGTACTGC ATTTGGTGCG TCCAGAGCAA TAGTGTGGCT ACAGAACAGG
    CGGGAAGCCA CTGTGGAGCG AACTCGGACC ACGAGCAGTG TACGGCGAGA TGATCCTGGG
    GAATTTAGAG TGGGTCGCCT CAAGCACGAA AGAGTGAAAG TTCCCCGTGG AGAGTCTCTG
    ATGGAGTGGG CCGAGAATGT CATGCAGATA CACGCAGATC GGAAGTCAGT TCTCGAGGTT
    GAATTTCTAG GAGAAGAAGG AACTGGCTTG GGACCCACTT TAGAGTTTTA TGCTCTTGTG
    GCGGCTGAGT TTCAGAGGAC TGACTTGGGG ACTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCATGTTGA TCTTGGAGGT GGATTGAAGC CTCCAGGATA CTATGTACAG
    CGGTCATGTG GACTGTTCAC AGCGCCATTC CCACAGGACA GTGATGAGCT TGAAAGGATC
    ACAAAACTCT TCCATTTCCT TGGGATTTTC TTGGCCAAAT GTATTCAGGA CAATAGACTG
    GTGGACTTGC CTATCTCTAA GCCTTTCTTT AAACTTATGT GCATGGGCGA CATTAAAAGC
    AATATGAGCA AACTAATCTA TGAGTCCCGC GGCGACAGAG ACTTGCACTG TACTGAAAGT
    CAGTCGGAAG CTTCTACAGA AGAAGGCCAT GACTCCCTCT CAGTGGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT CCTCGATCCC CCTAAGCCCA AACCCCCAGC CTGGTTTAAT
    GGGATTTTAA CTTGGGAAGA TTTTGAACTA GTAAATCCTC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT TAAGAGGCGC CAGATTCTAG GCAATAAAAG TCTTTCTGAA
    GATGAGAAGA ACACAAAGTT ACAGGAACTC GTGCTCAGGA ATCCATCAGG CTCGGGACCT
    CCACTGAGCA TCGAGGACTT AGGTTTAAAT TTCCAGTTTT GCCCATCTTC AAGAATATAT
    GGCTTTACAG CTGTGGATCT CAAGCCAAGT GGGGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTCTGTATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCTT TTCGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAGG AAGTCCAGAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GACATTATCA ATTACACGGA ACCTAAGCTT GGCTATACAC GTGACAGTCC TGGCTTCCTG
    CGGTTTGTGA GGGTTTTATG TGGCATGTCC TCAGATGAAA GGAAAGCATT CCTGCAGTTT
    ACCACTGGCT GTTCAACTCT GCCCCCAGGT GGACTGGCTA ACCTGCATCC GAGGCTCACA
    GTTGTACGAA AGGTTGATGC TACTGATGCG AGCTATCCAT CGGTCAACAC CTGTGTCCAC
    TATCTTAAGT TACCTGAGTA TTCTTCTGAG GAGATCATGA GAGAGCGCTT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242351.1
    CDS578..8407
    Translation

    Target ORF information:

    RefSeq Version XM_011244049.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244049.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGACCAGCA
    GTTGCATTAA TTCGAAAATT AATAGCTGTA CTAGAATCAA TTGAACGCCT ACCTCTCCAT
    TTGTATGACA CACCAGGATC CACGTATAAC CTCCAGATAC TTACAAGAAG ATTACGCTTT
    CGGTTGGAAC GTGCACCTGG TGAAACTTCA TTAATAGACC GGACAGGCAG GATGCTGAAG
    ATGGAGCCCT TAGCTACAGT TGAATCTCTG GAACAGTATC TGCTGAAAAT GGTGGCAAAG
    CAGTGGTACG ACTTTGACAG ATCCTCATTT GTTTTTGTTC GAAAATTAAG AGAAGGCCAA
    AATTTTATAT TCCGGCACCA ACATGACTTT GATGAGAATG GAATCATTTA CTGGATTGGA
    ACAAATGCAA AAACTGCGTA TGAATGGGTA AATCCTGCAG CTTATGGACT CGTAGTCGTC
    ACATCATCAG AAGGAAGGAA TCTGCCCTAT GGCCGCTTGG AGGACATACT AAGTCGAGAT
    AATTCAGCTT TAAATTGTCA CAGCAATGAT GATAAAAATG CCTGGTTTGC CATAGATCTG
    GGTGTCTGGG TAATACCTTC AGCATACACA CTTCGTCATG CTCGTGGCTA TGGAAGGTCT
    GCACTGAGAA ATTGGGTTTT CCAGGTATCC AAAGATGGAC AGAACTGGAC TTCTTTGTAT
    ACCCATGTTG ATGACTGCAG TCTCAATGAA CCAGGGTCAA CTGCAACATG GCCCCTTGAC
    CCAGCGAAGG ATGAAAAGCA AGGGTGGCGG CATGTCAGGC TGAAGCAGAT GGGGAAGAAC
    GCCAGTGGAC AGACGCACTA CCTCTCGCTC TCCGGCTTTG AACTCTATGG CACTGTGAAT
    GGCGTGTGCG AAGACCAACT AGGGAAAGCA GCTAAAGAAG CAGAAGCTAA TCTTAGAAGG
    CAGAGGCGCC TGGTGCGCTC TCAGGTCCTC AAGTACATGG TTCCAGGAGC ACGTGTTATC
    AGGGGCCTTG ATTGGAAGTG GCGAGATCAG GATGGCAGCC CGCAGGGAGA AGGCACAGTC
    ACAGGCGAGC TCCACAATGG CTGGATTGAT GTCACCTGGG ATGCTGGTGG CTCAAACTCT
    TACCGTATGG GCGCAGAAGG AAAATTTGAC CTCAAGCTTG CACCAGGGTA CGACCCTGAT
    ACAGTGGCAT CACCCAAACC TGTTTCATCC ACTGTTTCAG GCACAACGCA ATCATGGAGC
    AGCTTGGTGA AAAACAACTG TCCAGACAAG ACGTCTGCTG CTGCAGGCTC CTCAAGTAGA
    AAAGGGAGCA GCAGCTCTGT GTGTAGCGTG GCCAGTAGCA GCGACATCAG CTTGGCTTCG
    ACCAAAACGG AACGGAGATC AGAAATTGTA ATGGAACACA GTATAGTTTC AGGAGCTGAT
    GTCCATGAAC CAATTGTTGT TCTTTCATCT GCTGAAAACG TCCCTCAAAC AGAAGTAGGG
    TCATCTTCCA GTGCAAGCAC CAGCACCCTA ACAGCGGAAA CGGGAAGTGA AAATGCGGAA
    AGGAAGTTAG GCCCTGATAG TTCTGTTCGA GCTCCTGGGG AGTCTAGTGC GATATCCATG
    GGGATTGTTA GCGTTAGCTC TCCTGATGTT AGTTCGGTAT CTGAATTAAC CAATAAGGAA
    GCAGCTTCAC AACGACCCCT TAGCTCTTCG GCAAGTAACA GACTGTCGGT GAGCTCTCTG
    TTGGCAGCCG GGGCCCCTAT GAGCTCCAGT GCGAGTGTAC CTAACCTGTC CTCAAGAGAG
    ACATCTAGCT TGGAGAGCTT TGTCAGGAGA GTGGCAAACA TAGCACGGAC TAATGCCACC
    AACAACATGA ACCTAAGCCG AAGCAGCAGT GACAACAACA CTAACACTTT GGGGAGGAAC
    GTGATGAGCA CCGCAACTTC TCCACTTATG GGTGCTCAGA GTTTCCCTAA TTTGACCACA
    CCTGGTACTA CATCAACAGT GACAATGTCA ACATCGAGTG TTACGAGCAG CAGCAATGTA
    GCTACGGCAA CCACAGTTTT ATCAGTGGGT CAATCATTAA GTAATACTTT AACCACCAGC
    CTCACATCAA CCTCTAGTGA GAGTGACACA GGCCAGGAAG CAGAATACTC CTTATATGAT
    TTCCTCGACA GCTGCCGTGC CAGTACTCTC TTAGCTGAAC TAGATGATGA CGAGGATTTG
    CCTGAGCCTG ACGAGGAAGA CGATGAGAAT GAAGATGACA ATCAGGAGGA CCAAGAATAC
    GAGGAGGTTA TGATTCTGAG ACGTCCCTCC CTGCAACGCC GAGCTGGCTC TCGCTCTGAC
    GTAACACACC ATGTCGTCAC CTCCCAGCTC CCACAGGTCC CTTCTGGAGC AGGGAGCCGG
    CCTGTTGGGG AACAGGAAGA AGAAGAGTAT GAAACCAAAG GAGGCCGCCG CAGAGCCTGG
    GATGACGACT ATGTGCTAAA GCGCCAGTTT TCTGCACTGG TCCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CCAATGTCCA GCAGACAACT GACCTAGAAA TTCCTCCCCC AGGAACACCT
    CACTCAGAGC TCTTGGAGGA AGTTGAATGT ACTCCGTCAC CTCGCTTGGC TCTCACACTG
    AAAGTGACGG GGCTTGGAAC AACGCGGGAA GTTGAACTGC CACTTACCAA TTTCAGATCC
    ACCATCTTTT ACTATGTACA AAAACTGCTT CAACTGTCTT GTAATGGCAA TGTGAAGTCA
    GATAAACTTA GGCGTATTTG GGAGCCCACT TACACAATCA TGTATAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGGAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAACAG
    TACCTTGGCA CTGACGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATGCCGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTTAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGATTAAACC AGGGGGCTAT TTCTAGTCTT CAAAGTAGTG ATATTCTTAA TTTGACAAAA
    GAGCAGCCTC AGGCTAAAGC AGGCAACGGG CAGAGCCCCT GTGGAGTAGA GGACGTCCTT
    CAGCTTCTGC GGATTCTGTA TATAGTTGCA AGTGATCCTT ATTCAAGGAT ATCCCAAGAA
    GATGGCGATG AGCAGCCTCA GTTTACTTTT CCACCTGATG AATTTACAAG TAAAAAAATC
    ACCACAAAAA TTTTGCAGCA GATTGAGGAA CCGTTGGCAC TGGCCAGTGG GGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTGA TCCCATTCGA GACGAGGCAG
    CTTTATTTCA CCTGTACTGC ATTTGGTGCG TCCAGAGCAA TAGTGTGGCT ACAGAACAGG
    CGGGAAGCCA CTGTGGAGCG AACTCGGACC ACGAGCAGTG TACGGCGAGA TGATCCTGGG
    GAATTTAGAG TGGGTCGCCT CAAGCACGAA AGAGTGAAAG TTCCCCGTGG AGAGTCTCTG
    ATGGAGTGGG CCGAGAATGT CATGCAGATA CACGCAGATC GGAAGTCAGT TCTCGAGGTT
    GAATTTCTAG GAGAAGAAGG AACTGGCTTG GGACCCACTT TAGAGTTTTA TGCTCTTGTG
    GCGGCTGAGT TTCAGAGGAC TGACTTGGGG ACTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCATGTTGA TCTTGGAGGT GGATTGAAGC CTCCAGGATA CTATGTACAG
    CGGTCATGTG GACTGTTCAC AGCGCCATTC CCACAGGACA GTGATGAGCT TGAAAGGATC
    ACAAAACTCT TCCATTTCCT TGGGATTTTC TTGGCCAAAT GTATTCAGGA CAATAGACTG
    GTGGACTTGC CTATCTCTAA GCCTTTCTTT AAACTTATGT GCATGGGCGA CATTAAAAGC
    AATATGAGCA AACTAATCTA TGAGTCCCGC GGCGACAGAG ACTTGCACTG TACTGAAAGT
    CAGTCGGAAG CTTCTACAGA AGAAGGCCAT GACTCCCTCT CAGTGGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT CCTCGATCCC CCTAAGCCCA AACCCCCAGC CTGGTTTAAT
    GGGATTTTAA CTTGGGAAGA TTTTGAACTA GTAAATCCTC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT TAAGAGGCGC CAGATTCTAG GCAATAAAAG TCTTTCTGAA
    GATGAGAAGA ACACAAAGTT ACAGGAACTC GTGCTCAGGA ATCCATCAGG CTCGGGACCT
    CCACTGAGCA TCGAGGACTT AGGTTTAAAT TTCCAGTTTT GCCCATCTTC AAGAATATAT
    GGCTTTACAG CTGTGGATCT CAAGCCAAGT GGGGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTCTGTATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCTT TTCGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAGG AAGTCCAGAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GACATTATCA ATTACACGGA ACCTAAGCTT GGCTATACAC GTGACAGTCC TGGCTTCCTG
    CGGTTTGTGA GGGTTTTATG TGGCATGTCC TCAGATGAAA GGAAAGCATT CCTGCAGTTT
    ACCACTGGCT GTTCAACTCT GCCCCCAGGT GGACTGGCTA ACCTGCATCC GAGGCTCACA
    GTTGTACGAA AGGTTGATGC TACTGATGCG AGCTATCCAT CGGTCAACAC CTGTGTCCAC
    TATCTTAAGT TACCTGAGTA TTCTTCTGAG GAGATCATGA GAGAGCGCTT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72601
    Clone ID Related Accession (Same CDS sequence) XM_011244050.2 , XM_011244050.3
    Accession Version XM_011244050.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGGA GCAGCAGCTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGCT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCG
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCGATATCC
    ATGGGGATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCGG TATCTGAATT AACCAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCT TCGGCAAGTA ACAGACTGTC GGTGAGCTCT
    CTGTTGGCAG CCGGGGCCCC TATGAGCTCC AGTGCGAGTG TACCTAACCT GTCCTCAAGA
    GAGACATCTA GCTTGGAGAG CTTTGTCAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTAAG CCGAAGCAGC AGTGACAACA ACACTAACAC TTTGGGGAGG
    AACGTGATGA GCACCGCAAC TTCTCCACTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCGA GTGTTACGAG CAGCAGCAAT
    GTAGCTACGG CAACCACAGT TTTATCAGTG GGTCAATCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGCCAGG AAGCAGAATA CTCCTTATAT
    GATTTCCTCG ACAGCTGCCG TGCCAGTACT CTCTTAGCTG AACTAGATGA TGACGAGGAT
    TTGCCTGAGC CTGACGAGGA AGACGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCCTCCCTG CAACGCCGAG CTGGCTCTCG CTCTGACGTA
    ACACACCATG TCGTCACCTC CCAGCTCCCA CAGGTCCCTT CTGGAGCAGG GAGCCGGCCT
    GTTGGGGAAC AGGAAGAAGA AGAGTATGAA ACCAAAGGAG GCCGCCGCAG AGCCTGGGAT
    GACGACTATG TGCTAAAGCG CCAGTTTTCT GCACTGGTCC CTGCTTTTGA TCCTAGACCT
    GGTCGTACCA ATGTCCAGCA GACAACTGAC CTAGAAATTC CTCCCCCAGG AACACCTCAC
    TCAGAGCTCT TGGAGGAAGT TGAATGTACT CCGTCACCTC GCTTGGCTCT CACACTGAAA
    GTGACGGGGC TTGGAACAAC GCGGGAAGTT GAACTGCCAC TTACCAATTT CAGATCCACC
    ATCTTTTACT ATGTACAAAA ACTGCTTCAA CTGTCTTGTA ATGGCAATGT GAAGTCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACTTAC ACAATCATGT ATAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG GAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ACGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAT
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTTATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGA
    TTAAACCAGG GGGCTATTTC TAGTCTTCAA AGTAGTGATA TTCTTAATTT GACAAAAGAG
    CAGCCTCAGG CTAAAGCAGG CAACGGGCAG AGCCCCTGTG GAGTAGAGGA CGTCCTTCAG
    CTTCTGCGGA TTCTGTATAT AGTTGCAAGT GATCCTTATT CAAGGATATC CCAAGAAGAT
    GGCGATGAGC AGCCTCAGTT TACTTTTCCA CCTGATGAAT TTACAAGTAA AAAAATCACC
    ACAAAAATTT TGCAGCAGAT TGAGGAACCG TTGGCACTGG CCAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTCCTGATCC CATTCGAGAC GAGGCAGCTT
    TATTTCACCT GTACTGCATT TGGTGCGTCC AGAGCAATAG TGTGGCTACA GAACAGGCGG
    GAAGCCACTG TGGAGCGAAC TCGGACCACG AGCAGTGTAC GGCGAGATGA TCCTGGGGAA
    TTTAGAGTGG GTCGCCTCAA GCACGAAAGA GTGAAAGTTC CCCGTGGAGA GTCTCTGATG
    GAGTGGGCCG AGAATGTCAT GCAGATACAC GCAGATCGGA AGTCAGTTCT CGAGGTTGAA
    TTTCTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACTTTAG AGTTTTATGC TCTTGTGGCG
    GCTGAGTTTC AGAGGACTGA CTTGGGGACT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ATGTTGATCT TGGAGGTGGA TTGAAGCCTC CAGGATACTA TGTACAGCGG
    TCATGTGGAC TGTTCACAGC GCCATTCCCA CAGGACAGTG ATGAGCTTGA AAGGATCACA
    AAACTCTTCC ATTTCCTTGG GATTTTCTTG GCCAAATGTA TTCAGGACAA TAGACTGGTG
    GACTTGCCTA TCTCTAAGCC TTTCTTTAAA CTTATGTGCA TGGGCGACAT TAAAAGCAAT
    ATGAGCAAAC TAATCTATGA GTCCCGCGGC GACAGAGACT TGCACTGTAC TGAAAGTCAG
    TCGGAAGCTT CTACAGAAGA AGGCCATGAC TCCCTCTCAG TGGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATCCT CGATCCCCCT AAGCCCAAAC CCCCAGCCTG GTTTAATGGG
    ATTTTAACTT GGGAAGATTT TGAACTAGTA AATCCTCACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATTAA GAGGCGCCAG ATTCTAGGCA ATAAAAGTCT TTCTGAAGAT
    GAGAAGAACA CAAAGTTACA GGAACTCGTG CTCAGGAATC CATCAGGCTC GGGACCTCCA
    CTGAGCATCG AGGACTTAGG TTTAAATTTC CAGTTTTGCC CATCTTCAAG AATATATGGC
    TTTACAGCTG TGGATCTCAA GCCAAGTGGG GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTC TGTATGCATA CGGGTATTCA GAAACAGATG
    GAAGCTTTTC GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAGGAAG TCCAGATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAC
    ATTATCAATT ACACGGAACC TAAGCTTGGC TATACACGTG ACAGTCCTGG CTTCCTGCGG
    TTTGTGAGGG TTTTATGTGG CATGTCCTCA GATGAAAGGA AAGCATTCCT GCAGTTTACC
    ACTGGCTGTT CAACTCTGCC CCCAGGTGGA CTGGCTAACC TGCATCCGAG GCTCACAGTT
    GTACGAAAGG TTGATGCTAC TGATGCGAGC TATCCATCGG TCAACACCTG TGTCCACTAT
    CTTAAGTTAC CTGAGTATTC TTCTGAGGAG ATCATGAGAG AGCGCTTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242352.1
    CDS578..8404
    Translation

    Target ORF information:

    RefSeq Version XM_011244050.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244050.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGGA GCAGCAGCTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGCT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCG
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCGATATCC
    ATGGGGATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCGG TATCTGAATT AACCAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCT TCGGCAAGTA ACAGACTGTC GGTGAGCTCT
    CTGTTGGCAG CCGGGGCCCC TATGAGCTCC AGTGCGAGTG TACCTAACCT GTCCTCAAGA
    GAGACATCTA GCTTGGAGAG CTTTGTCAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTAAG CCGAAGCAGC AGTGACAACA ACACTAACAC TTTGGGGAGG
    AACGTGATGA GCACCGCAAC TTCTCCACTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCGA GTGTTACGAG CAGCAGCAAT
    GTAGCTACGG CAACCACAGT TTTATCAGTG GGTCAATCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGCCAGG AAGCAGAATA CTCCTTATAT
    GATTTCCTCG ACAGCTGCCG TGCCAGTACT CTCTTAGCTG AACTAGATGA TGACGAGGAT
    TTGCCTGAGC CTGACGAGGA AGACGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCCTCCCTG CAACGCCGAG CTGGCTCTCG CTCTGACGTA
    ACACACCATG TCGTCACCTC CCAGCTCCCA CAGGTCCCTT CTGGAGCAGG GAGCCGGCCT
    GTTGGGGAAC AGGAAGAAGA AGAGTATGAA ACCAAAGGAG GCCGCCGCAG AGCCTGGGAT
    GACGACTATG TGCTAAAGCG CCAGTTTTCT GCACTGGTCC CTGCTTTTGA TCCTAGACCT
    GGTCGTACCA ATGTCCAGCA GACAACTGAC CTAGAAATTC CTCCCCCAGG AACACCTCAC
    TCAGAGCTCT TGGAGGAAGT TGAATGTACT CCGTCACCTC GCTTGGCTCT CACACTGAAA
    GTGACGGGGC TTGGAACAAC GCGGGAAGTT GAACTGCCAC TTACCAATTT CAGATCCACC
    ATCTTTTACT ATGTACAAAA ACTGCTTCAA CTGTCTTGTA ATGGCAATGT GAAGTCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACTTAC ACAATCATGT ATAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG GAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ACGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAT
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTTATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGA
    TTAAACCAGG GGGCTATTTC TAGTCTTCAA AGTAGTGATA TTCTTAATTT GACAAAAGAG
    CAGCCTCAGG CTAAAGCAGG CAACGGGCAG AGCCCCTGTG GAGTAGAGGA CGTCCTTCAG
    CTTCTGCGGA TTCTGTATAT AGTTGCAAGT GATCCTTATT CAAGGATATC CCAAGAAGAT
    GGCGATGAGC AGCCTCAGTT TACTTTTCCA CCTGATGAAT TTACAAGTAA AAAAATCACC
    ACAAAAATTT TGCAGCAGAT TGAGGAACCG TTGGCACTGG CCAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTCCTGATCC CATTCGAGAC GAGGCAGCTT
    TATTTCACCT GTACTGCATT TGGTGCGTCC AGAGCAATAG TGTGGCTACA GAACAGGCGG
    GAAGCCACTG TGGAGCGAAC TCGGACCACG AGCAGTGTAC GGCGAGATGA TCCTGGGGAA
    TTTAGAGTGG GTCGCCTCAA GCACGAAAGA GTGAAAGTTC CCCGTGGAGA GTCTCTGATG
    GAGTGGGCCG AGAATGTCAT GCAGATACAC GCAGATCGGA AGTCAGTTCT CGAGGTTGAA
    TTTCTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACTTTAG AGTTTTATGC TCTTGTGGCG
    GCTGAGTTTC AGAGGACTGA CTTGGGGACT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ATGTTGATCT TGGAGGTGGA TTGAAGCCTC CAGGATACTA TGTACAGCGG
    TCATGTGGAC TGTTCACAGC GCCATTCCCA CAGGACAGTG ATGAGCTTGA AAGGATCACA
    AAACTCTTCC ATTTCCTTGG GATTTTCTTG GCCAAATGTA TTCAGGACAA TAGACTGGTG
    GACTTGCCTA TCTCTAAGCC TTTCTTTAAA CTTATGTGCA TGGGCGACAT TAAAAGCAAT
    ATGAGCAAAC TAATCTATGA GTCCCGCGGC GACAGAGACT TGCACTGTAC TGAAAGTCAG
    TCGGAAGCTT CTACAGAAGA AGGCCATGAC TCCCTCTCAG TGGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATCCT CGATCCCCCT AAGCCCAAAC CCCCAGCCTG GTTTAATGGG
    ATTTTAACTT GGGAAGATTT TGAACTAGTA AATCCTCACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATTAA GAGGCGCCAG ATTCTAGGCA ATAAAAGTCT TTCTGAAGAT
    GAGAAGAACA CAAAGTTACA GGAACTCGTG CTCAGGAATC CATCAGGCTC GGGACCTCCA
    CTGAGCATCG AGGACTTAGG TTTAAATTTC CAGTTTTGCC CATCTTCAAG AATATATGGC
    TTTACAGCTG TGGATCTCAA GCCAAGTGGG GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTC TGTATGCATA CGGGTATTCA GAAACAGATG
    GAAGCTTTTC GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAGGAAG TCCAGATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAC
    ATTATCAATT ACACGGAACC TAAGCTTGGC TATACACGTG ACAGTCCTGG CTTCCTGCGG
    TTTGTGAGGG TTTTATGTGG CATGTCCTCA GATGAAAGGA AAGCATTCCT GCAGTTTACC
    ACTGGCTGTT CAACTCTGCC CCCAGGTGGA CTGGCTAACC TGCATCCGAG GCTCACAGTT
    GTACGAAAGG TTGATGCTAC TGATGCGAGC TATCCATCGG TCAACACCTG TGTCCACTAT
    CTTAAGTTAC CTGAGTATTC TTCTGAGGAG ATCATGAGAG AGCGCTTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72602
    Clone ID Related Accession (Same CDS sequence) XM_011244051.3 , XM_011244051.2
    Accession Version XM_011244051.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCCT CCCTGCAACG CCGAGCTGGC TCTCGCTCTG ACGTAACACA CCATGTCGTC
    ACCTCCCAGC TCCCACAGGT CCCTTCTGGA GCAGGGAGCC GGCCTGTTGG GGAACAGGAA
    GAAGAAGAGT ATGAAACCAA AGGAGGCCGC CGCAGAGCCT GGGATGACGA CTATGTGCTA
    AAGCGCCAGT TTTCTGCACT GGTCCCTGCT TTTGATCCTA GACCTGGTCG TACCAATGTC
    CAGCAGACAA CTGACCTAGA AATTCCTCCC CCAGGAACAC CTCACTCAGA GCTCTTGGAG
    GAAGTTGAAT GTACTCCGTC ACCTCGCTTG GCTCTCACAC TGAAAGTGAC GGGGCTTGGA
    ACAACGCGGG AAGTTGAACT GCCACTTACC AATTTCAGAT CCACCATCTT TTACTATGTA
    CAAAAACTGC TTCAACTGTC TTGTAATGGC AATGTGAAGT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CTTACACAAT CATGTATAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGGAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGACGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGATGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTT
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGATTAAA CCAGGGGGCT
    ATTTCTAGTC TTCAAAGTAG TGATATTCTT AATTTGACAA AAGAGCAGCC TCAGGCTAAA
    GCAGGCAACG GGCAGAGCCC CTGTGGAGTA GAGGACGTCC TTCAGCTTCT GCGGATTCTG
    TATATAGTTG CAAGTGATCC TTATTCAAGG ATATCCCAAG AAGATGGCGA TGAGCAGCCT
    CAGTTTACTT TTCCACCTGA TGAATTTACA AGTAAAAAAA TCACCACAAA AATTTTGCAG
    CAGATTGAGG AACCGTTGGC ACTGGCCAGT GGGGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT GATCCCATTC GAGACGAGGC AGCTTTATTT CACCTGTACT
    GCATTTGGTG CGTCCAGAGC AATAGTGTGG CTACAGAACA GGCGGGAAGC CACTGTGGAG
    CGAACTCGGA CCACGAGCAG TGTACGGCGA GATGATCCTG GGGAATTTAG AGTGGGTCGC
    CTCAAGCACG AAAGAGTGAA AGTTCCCCGT GGAGAGTCTC TGATGGAGTG GGCCGAGAAT
    GTCATGCAGA TACACGCAGA TCGGAAGTCA GTTCTCGAGG TTGAATTTCT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC TTTAGAGTTT TATGCTCTTG TGGCGGCTGA GTTTCAGAGG
    ACTGACTTGG GGACTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCATGTT
    GATCTTGGAG GTGGATTGAA GCCTCCAGGA TACTATGTAC AGCGGTCATG TGGACTGTTC
    ACAGCGCCAT TCCCACAGGA CAGTGATGAG CTTGAAAGGA TCACAAAACT CTTCCATTTC
    CTTGGGATTT TCTTGGCCAA ATGTATTCAG GACAATAGAC TGGTGGACTT GCCTATCTCT
    AAGCCTTTCT TTAAACTTAT GTGCATGGGC GACATTAAAA GCAATATGAG CAAACTAATC
    TATGAGTCCC GCGGCGACAG AGACTTGCAC TGTACTGAAA GTCAGTCGGA AGCTTCTACA
    GAAGAAGGCC ATGACTCCCT CTCAGTGGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATCCTCGATC CCCCTAAGCC CAAACCCCCA GCCTGGTTTA ATGGGATTTT AACTTGGGAA
    GATTTTGAAC TAGTAAATCC TCACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATTAAGAGGC GCCAGATTCT AGGCAATAAA AGTCTTTCTG AAGATGAGAA GAACACAAAG
    TTACAGGAAC TCGTGCTCAG GAATCCATCA GGCTCGGGAC CTCCACTGAG CATCGAGGAC
    TTAGGTTTAA ATTTCCAGTT TTGCCCATCT TCAAGAATAT ATGGCTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGGGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTCTGTAT GCATACGGGT ATTCAGAAAC AGATGGAAGC TTTTCGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA GGAAGTCCAG
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGACATTAT CAATTACACG
    GAACCTAAGC TTGGCTATAC ACGTGACAGT CCTGGCTTCC TGCGGTTTGT GAGGGTTTTA
    TGTGGCATGT CCTCAGATGA AAGGAAAGCA TTCCTGCAGT TTACCACTGG CTGTTCAACT
    CTGCCCCCAG GTGGACTGGC TAACCTGCAT CCGAGGCTCA CAGTTGTACG AAAGGTTGAT
    GCTACTGATG CGAGCTATCC ATCGGTCAAC ACCTGTGTCC ACTATCTTAA GTTACCTGAG
    TATTCTTCTG AGGAGATCAT GAGAGAGCGC TTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242353.1
    CDS578..8269
    Translation

    Target ORF information:

    RefSeq Version XM_011244051.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244051.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCCT CCCTGCAACG CCGAGCTGGC TCTCGCTCTG ACGTAACACA CCATGTCGTC
    ACCTCCCAGC TCCCACAGGT CCCTTCTGGA GCAGGGAGCC GGCCTGTTGG GGAACAGGAA
    GAAGAAGAGT ATGAAACCAA AGGAGGCCGC CGCAGAGCCT GGGATGACGA CTATGTGCTA
    AAGCGCCAGT TTTCTGCACT GGTCCCTGCT TTTGATCCTA GACCTGGTCG TACCAATGTC
    CAGCAGACAA CTGACCTAGA AATTCCTCCC CCAGGAACAC CTCACTCAGA GCTCTTGGAG
    GAAGTTGAAT GTACTCCGTC ACCTCGCTTG GCTCTCACAC TGAAAGTGAC GGGGCTTGGA
    ACAACGCGGG AAGTTGAACT GCCACTTACC AATTTCAGAT CCACCATCTT TTACTATGTA
    CAAAAACTGC TTCAACTGTC TTGTAATGGC AATGTGAAGT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CTTACACAAT CATGTATAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGGAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGACGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGATGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTT
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGATTAAA CCAGGGGGCT
    ATTTCTAGTC TTCAAAGTAG TGATATTCTT AATTTGACAA AAGAGCAGCC TCAGGCTAAA
    GCAGGCAACG GGCAGAGCCC CTGTGGAGTA GAGGACGTCC TTCAGCTTCT GCGGATTCTG
    TATATAGTTG CAAGTGATCC TTATTCAAGG ATATCCCAAG AAGATGGCGA TGAGCAGCCT
    CAGTTTACTT TTCCACCTGA TGAATTTACA AGTAAAAAAA TCACCACAAA AATTTTGCAG
    CAGATTGAGG AACCGTTGGC ACTGGCCAGT GGGGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT GATCCCATTC GAGACGAGGC AGCTTTATTT CACCTGTACT
    GCATTTGGTG CGTCCAGAGC AATAGTGTGG CTACAGAACA GGCGGGAAGC CACTGTGGAG
    CGAACTCGGA CCACGAGCAG TGTACGGCGA GATGATCCTG GGGAATTTAG AGTGGGTCGC
    CTCAAGCACG AAAGAGTGAA AGTTCCCCGT GGAGAGTCTC TGATGGAGTG GGCCGAGAAT
    GTCATGCAGA TACACGCAGA TCGGAAGTCA GTTCTCGAGG TTGAATTTCT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC TTTAGAGTTT TATGCTCTTG TGGCGGCTGA GTTTCAGAGG
    ACTGACTTGG GGACTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCATGTT
    GATCTTGGAG GTGGATTGAA GCCTCCAGGA TACTATGTAC AGCGGTCATG TGGACTGTTC
    ACAGCGCCAT TCCCACAGGA CAGTGATGAG CTTGAAAGGA TCACAAAACT CTTCCATTTC
    CTTGGGATTT TCTTGGCCAA ATGTATTCAG GACAATAGAC TGGTGGACTT GCCTATCTCT
    AAGCCTTTCT TTAAACTTAT GTGCATGGGC GACATTAAAA GCAATATGAG CAAACTAATC
    TATGAGTCCC GCGGCGACAG AGACTTGCAC TGTACTGAAA GTCAGTCGGA AGCTTCTACA
    GAAGAAGGCC ATGACTCCCT CTCAGTGGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATCCTCGATC CCCCTAAGCC CAAACCCCCA GCCTGGTTTA ATGGGATTTT AACTTGGGAA
    GATTTTGAAC TAGTAAATCC TCACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATTAAGAGGC GCCAGATTCT AGGCAATAAA AGTCTTTCTG AAGATGAGAA GAACACAAAG
    TTACAGGAAC TCGTGCTCAG GAATCCATCA GGCTCGGGAC CTCCACTGAG CATCGAGGAC
    TTAGGTTTAA ATTTCCAGTT TTGCCCATCT TCAAGAATAT ATGGCTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGGGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTCTGTAT GCATACGGGT ATTCAGAAAC AGATGGAAGC TTTTCGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA GGAAGTCCAG
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGACATTAT CAATTACACG
    GAACCTAAGC TTGGCTATAC ACGTGACAGT CCTGGCTTCC TGCGGTTTGT GAGGGTTTTA
    TGTGGCATGT CCTCAGATGA AAGGAAAGCA TTCCTGCAGT TTACCACTGG CTGTTCAACT
    CTGCCCCCAG GTGGACTGGC TAACCTGCAT CCGAGGCTCA CAGTTGTACG AAAGGTTGAT
    GCTACTGATG CGAGCTATCC ATCGGTCAAC ACCTGTGTCC ACTATCTTAA GTTACCTGAG
    TATTCTTCTG AGGAGATCAT GAGAGAGCGC TTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78179
    Clone ID Related Accession (Same CDS sequence) XM_017315008.1 , XM_017315008.2
    Accession Version XM_017315008.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCCTCCCTGC AACGCCGAGC TGGCTCTCGC TCTGACGTAA CACACCATGT CGTCACCTCC
    CAGCTCCCAC AGGTCCCTTC TGGAGCAGGG AGCCGGCCTG TTGGGGAACA GGAAGAAGAA
    GAGTATGAAA CCAAAGGAGG CCGCCGCAGA GCCTGGGATG ACGACTATGT GCTAAAGCGC
    CAGTTTTCTG CACTGGTCCC TGCTTTTGAT CCTAGACCTG GTCGTACCAA TGTCCAGCAG
    ACAACTGACC TAGAAATTCC TCCCCCAGGA ACACCTCACT CAGAGCTCTT GGAGGAAGTT
    GAATGTACTC CGTCACCTCG CTTGGCTCTC ACACTGAAAG TGACGGGGCT TGGAACAACG
    CGGGAAGTTG AACTGCCACT TACCAATTTC AGATCCACCA TCTTTTACTA TGTACAAAAA
    CTGCTTCAAC TGTCTTGTAA TGGCAATGTG AAGTCAGATA AACTTAGGCG TATTTGGGAG
    CCCACTTACA CAATCATGTA TAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGG
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA CGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGATG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTTATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGAT TAAACCAGGG GGCTATTTCT
    AGTCTTCAAA GTAGTGATAT TCTTAATTTG ACAAAAGAGC AGCCTCAGGC TAAAGCAGGC
    AACGGGCAGA GCCCCTGTGG AGTAGAGGAC GTCCTTCAGC TTCTGCGGAT TCTGTATATA
    GTTGCAAGTG ATCCTTATTC AAGGATATCC CAAGAAGATG GCGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CTGATGAATT TACAAGTAAA AAAATCACCA CAAAAATTTT GCAGCAGATT
    GAGGAACCGT TGGCACTGGC CAGTGGGGCT CTGCCAGACT GGTGTGAACA GTTAACCAGC
    AAGTGTCCTT TCCTGATCCC ATTCGAGACG AGGCAGCTTT ATTTCACCTG TACTGCATTT
    GGTGCGTCCA GAGCAATAGT GTGGCTACAG AACAGGCGGG AAGCCACTGT GGAGCGAACT
    CGGACCACGA GCAGTGTACG GCGAGATGAT CCTGGGGAAT TTAGAGTGGG TCGCCTCAAG
    CACGAAAGAG TGAAAGTTCC CCGTGGAGAG TCTCTGATGG AGTGGGCCGA GAATGTCATG
    CAGATACACG CAGATCGGAA GTCAGTTCTC GAGGTTGAAT TTCTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACTTTAGA GTTTTATGCT CTTGTGGCGG CTGAGTTTCA GAGGACTGAC
    TTGGGGACTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA TGTTGATCTT
    GGAGGTGGAT TGAAGCCTCC AGGATACTAT GTACAGCGGT CATGTGGACT GTTCACAGCG
    CCATTCCCAC AGGACAGTGA TGAGCTTGAA AGGATCACAA AACTCTTCCA TTTCCTTGGG
    ATTTTCTTGG CCAAATGTAT TCAGGACAAT AGACTGGTGG ACTTGCCTAT CTCTAAGCCT
    TTCTTTAAAC TTATGTGCAT GGGCGACATT AAAAGCAATA TGAGCAAACT AATCTATGAG
    TCCCGCGGCG ACAGAGACTT GCACTGTACT GAAAGTCAGT CGGAAGCTTC TACAGAAGAA
    GGCCATGACT CCCTCTCAGT GGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATCCTC
    GATCCCCCTA AGCCCAAACC CCCAGCCTGG TTTAATGGGA TTTTAACTTG GGAAGATTTT
    GAACTAGTAA ATCCTCACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATTAAG
    AGGCGCCAGA TTCTAGGCAA TAAAAGTCTT TCTGAAGATG AGAAGAACAC AAAGTTACAG
    GAACTCGTGC TCAGGAATCC ATCAGGCTCG GGACCTCCAC TGAGCATCGA GGACTTAGGT
    TTAAATTTCC AGTTTTGCCC ATCTTCAAGA ATATATGGCT TTACAGCTGT GGATCTCAAG
    CCAAGTGGGG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTCT GTATGCATAC GGGTATTCAG AAACAGATGG AAGCTTTTCG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAGGAAGT CCAGATGATT
    CTTTGTGGAA ATCAGTCACC ATCCTGGGCA GCAGAGGACA TTATCAATTA CACGGAACCT
    AAGCTTGGCT ATACACGTGA CAGTCCTGGC TTCCTGCGGT TTGTGAGGGT TTTATGTGGC
    ATGTCCTCAG ATGAAAGGAA AGCATTCCTG CAGTTTACCA CTGGCTGTTC AACTCTGCCC
    CCAGGTGGAC TGGCTAACCT GCATCCGAGG CTCACAGTTG TACGAAAGGT TGATGCTACT
    GATGCGAGCT ATCCATCGGT CAACACCTGT GTCCACTATC TTAAGTTACC TGAGTATTCT
    TCTGAGGAGA TCATGAGAGA GCGCTTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170497.1
    CDS357..8042
    Translation

    Target ORF information:

    RefSeq Version XM_017315008.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain containing 1 (Hectd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCCTCCCTGC AACGCCGAGC TGGCTCTCGC TCTGACGTAA CACACCATGT CGTCACCTCC
    CAGCTCCCAC AGGTCCCTTC TGGAGCAGGG AGCCGGCCTG TTGGGGAACA GGAAGAAGAA
    GAGTATGAAA CCAAAGGAGG CCGCCGCAGA GCCTGGGATG ACGACTATGT GCTAAAGCGC
    CAGTTTTCTG CACTGGTCCC TGCTTTTGAT CCTAGACCTG GTCGTACCAA TGTCCAGCAG
    ACAACTGACC TAGAAATTCC TCCCCCAGGA ACACCTCACT CAGAGCTCTT GGAGGAAGTT
    GAATGTACTC CGTCACCTCG CTTGGCTCTC ACACTGAAAG TGACGGGGCT TGGAACAACG
    CGGGAAGTTG AACTGCCACT TACCAATTTC AGATCCACCA TCTTTTACTA TGTACAAAAA
    CTGCTTCAAC TGTCTTGTAA TGGCAATGTG AAGTCAGATA AACTTAGGCG TATTTGGGAG
    CCCACTTACA CAATCATGTA TAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGG
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA CGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGATG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTTATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGAT TAAACCAGGG GGCTATTTCT
    AGTCTTCAAA GTAGTGATAT TCTTAATTTG ACAAAAGAGC AGCCTCAGGC TAAAGCAGGC
    AACGGGCAGA GCCCCTGTGG AGTAGAGGAC GTCCTTCAGC TTCTGCGGAT TCTGTATATA
    GTTGCAAGTG ATCCTTATTC AAGGATATCC CAAGAAGATG GCGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CTGATGAATT TACAAGTAAA AAAATCACCA CAAAAATTTT GCAGCAGATT
    GAGGAACCGT TGGCACTGGC CAGTGGGGCT CTGCCAGACT GGTGTGAACA GTTAACCAGC
    AAGTGTCCTT TCCTGATCCC ATTCGAGACG AGGCAGCTTT ATTTCACCTG TACTGCATTT
    GGTGCGTCCA GAGCAATAGT GTGGCTACAG AACAGGCGGG AAGCCACTGT GGAGCGAACT
    CGGACCACGA GCAGTGTACG GCGAGATGAT CCTGGGGAAT TTAGAGTGGG TCGCCTCAAG
    CACGAAAGAG TGAAAGTTCC CCGTGGAGAG TCTCTGATGG AGTGGGCCGA GAATGTCATG
    CAGATACACG CAGATCGGAA GTCAGTTCTC GAGGTTGAAT TTCTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACTTTAGA GTTTTATGCT CTTGTGGCGG CTGAGTTTCA GAGGACTGAC
    TTGGGGACTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA TGTTGATCTT
    GGAGGTGGAT TGAAGCCTCC AGGATACTAT GTACAGCGGT CATGTGGACT GTTCACAGCG
    CCATTCCCAC AGGACAGTGA TGAGCTTGAA AGGATCACAA AACTCTTCCA TTTCCTTGGG
    ATTTTCTTGG CCAAATGTAT TCAGGACAAT AGACTGGTGG ACTTGCCTAT CTCTAAGCCT
    TTCTTTAAAC TTATGTGCAT GGGCGACATT AAAAGCAATA TGAGCAAACT AATCTATGAG
    TCCCGCGGCG ACAGAGACTT GCACTGTACT GAAAGTCAGT CGGAAGCTTC TACAGAAGAA
    GGCCATGACT CCCTCTCAGT GGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATCCTC
    GATCCCCCTA AGCCCAAACC CCCAGCCTGG TTTAATGGGA TTTTAACTTG GGAAGATTTT
    GAACTAGTAA ATCCTCACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATTAAG
    AGGCGCCAGA TTCTAGGCAA TAAAAGTCTT TCTGAAGATG AGAAGAACAC AAAGTTACAG
    GAACTCGTGC TCAGGAATCC ATCAGGCTCG GGACCTCCAC TGAGCATCGA GGACTTAGGT
    TTAAATTTCC AGTTTTGCCC ATCTTCAAGA ATATATGGCT TTACAGCTGT GGATCTCAAG
    CCAAGTGGGG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTCT GTATGCATAC GGGTATTCAG AAACAGATGG AAGCTTTTCG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAGGAAGT CCAGATGATT
    CTTTGTGGAA ATCAGTCACC ATCCTGGGCA GCAGAGGACA TTATCAATTA CACGGAACCT
    AAGCTTGGCT ATACACGTGA CAGTCCTGGC TTCCTGCGGT TTGTGAGGGT TTTATGTGGC
    ATGTCCTCAG ATGAAAGGAA AGCATTCCTG CAGTTTACCA CTGGCTGTTC AACTCTGCCC
    CCAGGTGGAC TGGCTAACCT GCATCCGAGG CTCACAGTTG TACGAAAGGT TGATGCTACT
    GATGCGAGCT ATCCATCGGT CAACACCTGT GTCCACTATC TTAAGTTACC TGAGTATTCT
    TCTGAGGAGA TCATGAGAGA GCGCTTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu78179
    Clone ID Related Accession (Same CDS sequence) XM_017315008.1 , XM_017315008.2
    Accession Version XM_017315008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCCTCCCTGC AACGCCGAGC TGGCTCTCGC TCTGACGTAA CACACCATGT CGTCACCTCC
    CAGCTCCCAC AGGTCCCTTC TGGAGCAGGG AGCCGGCCTG TTGGGGAACA GGAAGAAGAA
    GAGTATGAAA CCAAAGGAGG CCGCCGCAGA GCCTGGGATG ACGACTATGT GCTAAAGCGC
    CAGTTTTCTG CACTGGTCCC TGCTTTTGAT CCTAGACCTG GTCGTACCAA TGTCCAGCAG
    ACAACTGACC TAGAAATTCC TCCCCCAGGA ACACCTCACT CAGAGCTCTT GGAGGAAGTT
    GAATGTACTC CGTCACCTCG CTTGGCTCTC ACACTGAAAG TGACGGGGCT TGGAACAACG
    CGGGAAGTTG AACTGCCACT TACCAATTTC AGATCCACCA TCTTTTACTA TGTACAAAAA
    CTGCTTCAAC TGTCTTGTAA TGGCAATGTG AAGTCAGATA AACTTAGGCG TATTTGGGAG
    CCCACTTACA CAATCATGTA TAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGG
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA CGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGATG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTTATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGAT TAAACCAGGG GGCTATTTCT
    AGTCTTCAAA GTAGTGATAT TCTTAATTTG ACAAAAGAGC AGCCTCAGGC TAAAGCAGGC
    AACGGGCAGA GCCCCTGTGG AGTAGAGGAC GTCCTTCAGC TTCTGCGGAT TCTGTATATA
    GTTGCAAGTG ATCCTTATTC AAGGATATCC CAAGAAGATG GCGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CTGATGAATT TACAAGTAAA AAAATCACCA CAAAAATTTT GCAGCAGATT
    GAGGAACCGT TGGCACTGGC CAGTGGGGCT CTGCCAGACT GGTGTGAACA GTTAACCAGC
    AAGTGTCCTT TCCTGATCCC ATTCGAGACG AGGCAGCTTT ATTTCACCTG TACTGCATTT
    GGTGCGTCCA GAGCAATAGT GTGGCTACAG AACAGGCGGG AAGCCACTGT GGAGCGAACT
    CGGACCACGA GCAGTGTACG GCGAGATGAT CCTGGGGAAT TTAGAGTGGG TCGCCTCAAG
    CACGAAAGAG TGAAAGTTCC CCGTGGAGAG TCTCTGATGG AGTGGGCCGA GAATGTCATG
    CAGATACACG CAGATCGGAA GTCAGTTCTC GAGGTTGAAT TTCTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACTTTAGA GTTTTATGCT CTTGTGGCGG CTGAGTTTCA GAGGACTGAC
    TTGGGGACTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA TGTTGATCTT
    GGAGGTGGAT TGAAGCCTCC AGGATACTAT GTACAGCGGT CATGTGGACT GTTCACAGCG
    CCATTCCCAC AGGACAGTGA TGAGCTTGAA AGGATCACAA AACTCTTCCA TTTCCTTGGG
    ATTTTCTTGG CCAAATGTAT TCAGGACAAT AGACTGGTGG ACTTGCCTAT CTCTAAGCCT
    TTCTTTAAAC TTATGTGCAT GGGCGACATT AAAAGCAATA TGAGCAAACT AATCTATGAG
    TCCCGCGGCG ACAGAGACTT GCACTGTACT GAAAGTCAGT CGGAAGCTTC TACAGAAGAA
    GGCCATGACT CCCTCTCAGT GGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATCCTC
    GATCCCCCTA AGCCCAAACC CCCAGCCTGG TTTAATGGGA TTTTAACTTG GGAAGATTTT
    GAACTAGTAA ATCCTCACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATTAAG
    AGGCGCCAGA TTCTAGGCAA TAAAAGTCTT TCTGAAGATG AGAAGAACAC AAAGTTACAG
    GAACTCGTGC TCAGGAATCC ATCAGGCTCG GGACCTCCAC TGAGCATCGA GGACTTAGGT
    TTAAATTTCC AGTTTTGCCC ATCTTCAAGA ATATATGGCT TTACAGCTGT GGATCTCAAG
    CCAAGTGGGG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTCT GTATGCATAC GGGTATTCAG AAACAGATGG AAGCTTTTCG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAGGAAGT CCAGATGATT
    CTTTGTGGAA ATCAGTCACC ATCCTGGGCA GCAGAGGACA TTATCAATTA CACGGAACCT
    AAGCTTGGCT ATACACGTGA CAGTCCTGGC TTCCTGCGGT TTGTGAGGGT TTTATGTGGC
    ATGTCCTCAG ATGAAAGGAA AGCATTCCTG CAGTTTACCA CTGGCTGTTC AACTCTGCCC
    CCAGGTGGAC TGGCTAACCT GCATCCGAGG CTCACAGTTG TACGAAAGGT TGATGCTACT
    GATGCGAGCT ATCCATCGGT CAACACCTGT GTCCACTATC TTAAGTTACC TGAGTATTCT
    TCTGAGGAGA TCATGAGAGA GCGCTTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170497.1
    CDS578..8263
    Translation

    Target ORF information:

    RefSeq Version XM_017315008.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGAT TCTGAGACGT
    CCCTCCCTGC AACGCCGAGC TGGCTCTCGC TCTGACGTAA CACACCATGT CGTCACCTCC
    CAGCTCCCAC AGGTCCCTTC TGGAGCAGGG AGCCGGCCTG TTGGGGAACA GGAAGAAGAA
    GAGTATGAAA CCAAAGGAGG CCGCCGCAGA GCCTGGGATG ACGACTATGT GCTAAAGCGC
    CAGTTTTCTG CACTGGTCCC TGCTTTTGAT CCTAGACCTG GTCGTACCAA TGTCCAGCAG
    ACAACTGACC TAGAAATTCC TCCCCCAGGA ACACCTCACT CAGAGCTCTT GGAGGAAGTT
    GAATGTACTC CGTCACCTCG CTTGGCTCTC ACACTGAAAG TGACGGGGCT TGGAACAACG
    CGGGAAGTTG AACTGCCACT TACCAATTTC AGATCCACCA TCTTTTACTA TGTACAAAAA
    CTGCTTCAAC TGTCTTGTAA TGGCAATGTG AAGTCAGATA AACTTAGGCG TATTTGGGAG
    CCCACTTACA CAATCATGTA TAGAGAAATG AAGGATTCTG ATAAAGAAAA GGAAAATGGG
    AAAATGGGTT GCTGGTCTAT AGAGCATGTG GAACAGTACC TTGGCACTGA CGAATTACCA
    AAGAATGACT TGATAACCTA CCTGCAGAAG AATGCAGATG CCGCTTTCCT GCGCCACTGG
    AAATTAACTG GCACTAATAA AAGTATTAGG AAAAACAGAA ATTGTTCTCA GCTTATAGCT
    GCATATAAGG ATTTTTGTGA GCATGGAACA AAGTCTGGAT TAAACCAGGG GGCTATTTCT
    AGTCTTCAAA GTAGTGATAT TCTTAATTTG ACAAAAGAGC AGCCTCAGGC TAAAGCAGGC
    AACGGGCAGA GCCCCTGTGG AGTAGAGGAC GTCCTTCAGC TTCTGCGGAT TCTGTATATA
    GTTGCAAGTG ATCCTTATTC AAGGATATCC CAAGAAGATG GCGATGAGCA GCCTCAGTTT
    ACTTTTCCAC CTGATGAATT TACAAGTAAA AAAATCACCA CAAAAATTTT GCAGCAGATT
    GAGGAACCGT TGGCACTGGC CAGTGGGGCT CTGCCAGACT GGTGTGAACA GTTAACCAGC
    AAGTGTCCTT TCCTGATCCC ATTCGAGACG AGGCAGCTTT ATTTCACCTG TACTGCATTT
    GGTGCGTCCA GAGCAATAGT GTGGCTACAG AACAGGCGGG AAGCCACTGT GGAGCGAACT
    CGGACCACGA GCAGTGTACG GCGAGATGAT CCTGGGGAAT TTAGAGTGGG TCGCCTCAAG
    CACGAAAGAG TGAAAGTTCC CCGTGGAGAG TCTCTGATGG AGTGGGCCGA GAATGTCATG
    CAGATACACG CAGATCGGAA GTCAGTTCTC GAGGTTGAAT TTCTAGGAGA AGAAGGAACT
    GGCTTGGGAC CCACTTTAGA GTTTTATGCT CTTGTGGCGG CTGAGTTTCA GAGGACTGAC
    TTGGGGACTT GGCTTTGTGA TGATAATTTT CCAGATGATG AATCTCGTCA TGTTGATCTT
    GGAGGTGGAT TGAAGCCTCC AGGATACTAT GTACAGCGGT CATGTGGACT GTTCACAGCG
    CCATTCCCAC AGGACAGTGA TGAGCTTGAA AGGATCACAA AACTCTTCCA TTTCCTTGGG
    ATTTTCTTGG CCAAATGTAT TCAGGACAAT AGACTGGTGG ACTTGCCTAT CTCTAAGCCT
    TTCTTTAAAC TTATGTGCAT GGGCGACATT AAAAGCAATA TGAGCAAACT AATCTATGAG
    TCCCGCGGCG ACAGAGACTT GCACTGTACT GAAAGTCAGT CGGAAGCTTC TACAGAAGAA
    GGCCATGACT CCCTCTCAGT GGGAAGCTTT GAAGAGGATT CAAAATCAGA ATTTATCCTC
    GATCCCCCTA AGCCCAAACC CCCAGCCTGG TTTAATGGGA TTTTAACTTG GGAAGATTTT
    GAACTAGTAA ATCCTCACAG AGCCAGATTT TTAAAAGAAA TTAAAGACCT TGCTATTAAG
    AGGCGCCAGA TTCTAGGCAA TAAAAGTCTT TCTGAAGATG AGAAGAACAC AAAGTTACAG
    GAACTCGTGC TCAGGAATCC ATCAGGCTCG GGACCTCCAC TGAGCATCGA GGACTTAGGT
    TTAAATTTCC AGTTTTGCCC ATCTTCAAGA ATATATGGCT TTACAGCTGT GGATCTCAAG
    CCAAGTGGGG AAGATGAGAT GATAACAATG GATAATGCAG AAGAATATGT GGATTTGATG
    TTTGACTTCT GTATGCATAC GGGTATTCAG AAACAGATGG AAGCTTTTCG AGATGGGTTT
    AATAAAGTTT TTCCAATGGA GAAATTAAGT TCCTTCAGCC ATGAGGAAGT CCAGATGATT
    CTTTGTGGAA ATCAGTCACC ATCCTGGGCA GCAGAGGACA TTATCAATTA CACGGAACCT
    AAGCTTGGCT ATACACGTGA CAGTCCTGGC TTCCTGCGGT TTGTGAGGGT TTTATGTGGC
    ATGTCCTCAG ATGAAAGGAA AGCATTCCTG CAGTTTACCA CTGGCTGTTC AACTCTGCCC
    CCAGGTGGAC TGGCTAACCT GCATCCGAGG CTCACAGTTG TACGAAAGGT TGATGCTACT
    GATGCGAGCT ATCCATCGGT CAACACCTGT GTCCACTATC TTAAGTTACC TGAGTATTCT
    TCTGAGGAGA TCATGAGAGA GCGCTTGCTA GCTGCTACAA TGGAGAAAGG CTTTCATCTC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu11077
    Clone ID Related Accession (Same CDS sequence) NM_144788.2
    Accession Version NM_144788.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157213.2 and AC159644.3. On or before Sep 20, 2008 this sequence version replaced XM_915167.3, XM_974905.2, NM_144788.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

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    1021
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    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGGA GCAGCAGCTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGCT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCG
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCGATATCC
    ATGGGGATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCGG TATCTGAATT AACCAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCT TCGGCAAGTA ACAGACTGTC GGTGAGCTCT
    CTGTTGGCAG CCGGGGCCCC TATGAGCTCC AGTGCGAGTG TACCTAACCT GTCCTCAAGA
    GAGACATCTA GCTTGGAGAG CTTTGTCAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTAAG CCGAAGCAGC AGTGACAACA ACACTAACAC TTTGGGGAGG
    AACGTGATGA GCACCGCAAC TTCTCCACTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCGA GTGTTACGAG CAGCAGCAAT
    GTAGCTACGG CAACCACAGT TTTATCAGTG GGTCAATCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGCCAGG AAGCAGAATA CTCCTTATAT
    GATTTCCTCG ACAGCTGCCG TGCCAGTACT CTCTTAGCTG AACTAGATGA TGACGAGGAT
    TTGCCTGAGC CTGACGAGGA AGACGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCC TCCCTGCAAC GCCGAGCTGG CTCTCGCTCT
    GACGTAACAC ACCATGTCGT CACCTCCCAG CTCCCACAGG TCCCTTCTGG AGCAGGGAGC
    CGGCCTGTTG GGGAACAGGA AGAAGAAGAG TATGAAACCA AAGGAGGCCG CCGCAGAGCC
    TGGGATGACG ACTATGTGCT AAAGCGCCAG TTTTCTGCAC TGGTCCCTGC TTTTGATCCT
    AGACCTGGTC GTACCAATGT CCAGCAGACA ACTGACCTAG AAATTCCTCC CCCAGGAACA
    CCTCACTCAG AGCTCTTGGA GGAAGTTGAA TGTACTCCGT CACCTCGCTT GGCTCTCACA
    CTGAAAGTGA CGGGGCTTGG AACAACGCGG GAAGTTGAAC TGCCACTTAC CAATTTCAGA
    TCCACCATCT TTTACTATGT ACAAAAACTG CTTCAACTGT CTTGTAATGG CAATGTGAAG
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACTTACACAA TCATGTATAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGGAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGACGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGATGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT TATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGATTAA ACCAGGGGGC TATTTCTAGT CTTCAAAGTA GTGATATTCT TAATTTGACA
    AAAGAGCAGC CTCAGGCTAA AGCAGGCAAC GGGCAGAGCC CCTGTGGAGT AGAGGACGTC
    CTTCAGCTTC TGCGGATTCT GTATATAGTT GCAAGTGATC CTTATTCAAG GATATCCCAA
    GAAGATGGCG ATGAGCAGCC TCAGTTTACT TTTCCACCTG ATGAATTTAC AAGTAAAAAA
    ATCACCACAA AAATTTTGCA GCAGATTGAG GAACCGTTGG CACTGGCCAG TGGGGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTCC TGATCCCATT CGAGACGAGG
    CAGCTTTATT TCACCTGTAC TGCATTTGGT GCGTCCAGAG CAATAGTGTG GCTACAGAAC
    AGGCGGGAAG CCACTGTGGA GCGAACTCGG ACCACGAGCA GTGTACGGCG AGATGATCCT
    GGGGAATTTA GAGTGGGTCG CCTCAAGCAC GAAAGAGTGA AAGTTCCCCG TGGAGAGTCT
    CTGATGGAGT GGGCCGAGAA TGTCATGCAG ATACACGCAG ATCGGAAGTC AGTTCTCGAG
    GTTGAATTTC TAGGAGAAGA AGGAACTGGC TTGGGACCCA CTTTAGAGTT TTATGCTCTT
    GTGGCGGCTG AGTTTCAGAG GACTGACTTG GGGACTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AGCCTCCAGG ATACTATGTA
    CAGCGGTCAT GTGGACTGTT CACAGCGCCA TTCCCACAGG ACAGTGATGA GCTTGAAAGG
    ATCACAAAAC TCTTCCATTT CCTTGGGATT TTCTTGGCCA AATGTATTCA GGACAATAGA
    CTGGTGGACT TGCCTATCTC TAAGCCTTTC TTTAAACTTA TGTGCATGGG CGACATTAAA
    AGCAATATGA GCAAACTAAT CTATGAGTCC CGCGGCGACA GAGACTTGCA CTGTACTGAA
    AGTCAGTCGG AAGCTTCTAC AGAAGAAGGC CATGACTCCC TCTCAGTGGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATCCTCGAT CCCCCTAAGC CCAAACCCCC AGCCTGGTTT
    AATGGGATTT TAACTTGGGA AGATTTTGAA CTAGTAAATC CTCACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATTAAGAGG CGCCAGATTC TAGGCAATAA AAGTCTTTCT
    GAAGATGAGA AGAACACAAA GTTACAGGAA CTCGTGCTCA GGAATCCATC AGGCTCGGGA
    CCTCCACTGA GCATCGAGGA CTTAGGTTTA AATTTCCAGT TTTGCCCATC TTCAAGAATA
    TATGGCTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTCTGTA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CTTTTCGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AGGAAGTCCA GATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGACATTA TCAATTACAC GGAACCTAAG CTTGGCTATA CACGTGACAG TCCTGGCTTC
    CTGCGGTTTG TGAGGGTTTT ATGTGGCATG TCCTCAGATG AAAGGAAAGC ATTCCTGCAG
    TTTACCACTG GCTGTTCAAC TCTGCCCCCA GGTGGACTGG CTAACCTGCA TCCGAGGCTC
    ACAGTTGTAC GAAAGGTTGA TGCTACTGAT GCGAGCTATC CATCGGTCAA CACCTGTGTC
    CACTATCTTA AGTTACCTGA GTATTCTTCT GAGGAGATCA TGAGAGAGCG CTTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_659037.2
    CDS357..8189
    Translation

    Target ORF information:

    RefSeq Version NM_144788.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_144788.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGGA GCAGCAGCTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGCT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCG
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCGATATCC
    ATGGGGATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCGG TATCTGAATT AACCAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCT TCGGCAAGTA ACAGACTGTC GGTGAGCTCT
    CTGTTGGCAG CCGGGGCCCC TATGAGCTCC AGTGCGAGTG TACCTAACCT GTCCTCAAGA
    GAGACATCTA GCTTGGAGAG CTTTGTCAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTAAG CCGAAGCAGC AGTGACAACA ACACTAACAC TTTGGGGAGG
    AACGTGATGA GCACCGCAAC TTCTCCACTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCGA GTGTTACGAG CAGCAGCAAT
    GTAGCTACGG CAACCACAGT TTTATCAGTG GGTCAATCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGCCAGG AAGCAGAATA CTCCTTATAT
    GATTTCCTCG ACAGCTGCCG TGCCAGTACT CTCTTAGCTG AACTAGATGA TGACGAGGAT
    TTGCCTGAGC CTGACGAGGA AGACGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCC TCCCTGCAAC GCCGAGCTGG CTCTCGCTCT
    GACGTAACAC ACCATGTCGT CACCTCCCAG CTCCCACAGG TCCCTTCTGG AGCAGGGAGC
    CGGCCTGTTG GGGAACAGGA AGAAGAAGAG TATGAAACCA AAGGAGGCCG CCGCAGAGCC
    TGGGATGACG ACTATGTGCT AAAGCGCCAG TTTTCTGCAC TGGTCCCTGC TTTTGATCCT
    AGACCTGGTC GTACCAATGT CCAGCAGACA ACTGACCTAG AAATTCCTCC CCCAGGAACA
    CCTCACTCAG AGCTCTTGGA GGAAGTTGAA TGTACTCCGT CACCTCGCTT GGCTCTCACA
    CTGAAAGTGA CGGGGCTTGG AACAACGCGG GAAGTTGAAC TGCCACTTAC CAATTTCAGA
    TCCACCATCT TTTACTATGT ACAAAAACTG CTTCAACTGT CTTGTAATGG CAATGTGAAG
    TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACTTACACAA TCATGTATAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGGAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGACGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCAGATGCCG CTTTCCTGCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT TATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG
    TCTGGATTAA ACCAGGGGGC TATTTCTAGT CTTCAAAGTA GTGATATTCT TAATTTGACA
    AAAGAGCAGC CTCAGGCTAA AGCAGGCAAC GGGCAGAGCC CCTGTGGAGT AGAGGACGTC
    CTTCAGCTTC TGCGGATTCT GTATATAGTT GCAAGTGATC CTTATTCAAG GATATCCCAA
    GAAGATGGCG ATGAGCAGCC TCAGTTTACT TTTCCACCTG ATGAATTTAC AAGTAAAAAA
    ATCACCACAA AAATTTTGCA GCAGATTGAG GAACCGTTGG CACTGGCCAG TGGGGCTCTG
    CCAGACTGGT GTGAACAGTT AACCAGCAAG TGTCCTTTCC TGATCCCATT CGAGACGAGG
    CAGCTTTATT TCACCTGTAC TGCATTTGGT GCGTCCAGAG CAATAGTGTG GCTACAGAAC
    AGGCGGGAAG CCACTGTGGA GCGAACTCGG ACCACGAGCA GTGTACGGCG AGATGATCCT
    GGGGAATTTA GAGTGGGTCG CCTCAAGCAC GAAAGAGTGA AAGTTCCCCG TGGAGAGTCT
    CTGATGGAGT GGGCCGAGAA TGTCATGCAG ATACACGCAG ATCGGAAGTC AGTTCTCGAG
    GTTGAATTTC TAGGAGAAGA AGGAACTGGC TTGGGACCCA CTTTAGAGTT TTATGCTCTT
    GTGGCGGCTG AGTTTCAGAG GACTGACTTG GGGACTTGGC TTTGTGATGA TAATTTTCCA
    GATGATGAAT CTCGTCATGT TGATCTTGGA GGTGGATTGA AGCCTCCAGG ATACTATGTA
    CAGCGGTCAT GTGGACTGTT CACAGCGCCA TTCCCACAGG ACAGTGATGA GCTTGAAAGG
    ATCACAAAAC TCTTCCATTT CCTTGGGATT TTCTTGGCCA AATGTATTCA GGACAATAGA
    CTGGTGGACT TGCCTATCTC TAAGCCTTTC TTTAAACTTA TGTGCATGGG CGACATTAAA
    AGCAATATGA GCAAACTAAT CTATGAGTCC CGCGGCGACA GAGACTTGCA CTGTACTGAA
    AGTCAGTCGG AAGCTTCTAC AGAAGAAGGC CATGACTCCC TCTCAGTGGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATCCTCGAT CCCCCTAAGC CCAAACCCCC AGCCTGGTTT
    AATGGGATTT TAACTTGGGA AGATTTTGAA CTAGTAAATC CTCACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATTAAGAGG CGCCAGATTC TAGGCAATAA AAGTCTTTCT
    GAAGATGAGA AGAACACAAA GTTACAGGAA CTCGTGCTCA GGAATCCATC AGGCTCGGGA
    CCTCCACTGA GCATCGAGGA CTTAGGTTTA AATTTCCAGT TTTGCCCATC TTCAAGAATA
    TATGGCTTTA CAGCTGTGGA TCTCAAGCCA AGTGGGGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTCTGTA TGCATACGGG TATTCAGAAA
    CAGATGGAAG CTTTTCGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AGGAAGTCCA GATGATTCTT TGTGGAAATC AGTCACCATC CTGGGCAGCA
    GAGGACATTA TCAATTACAC GGAACCTAAG CTTGGCTATA CACGTGACAG TCCTGGCTTC
    CTGCGGTTTG TGAGGGTTTT ATGTGGCATG TCCTCAGATG AAAGGAAAGC ATTCCTGCAG
    TTTACCACTG GCTGTTCAAC TCTGCCCCCA GGTGGACTGG CTAACCTGCA TCCGAGGCTC
    ACAGTTGTAC GAAAGGTTGA TGCTACTGAT GCGAGCTATC CATCGGTCAA CACCTGTGTC
    CACTATCTTA AGTTACCTGA GTATTCTTCT GAGGAGATCA TGAGAGAGCG CTTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72600
    Clone ID Related Accession (Same CDS sequence) XM_011244049.3 , XM_011244049.2
    Accession Version XM_011244049.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGACCAGCA
    GTTGCATTAA TTCGAAAATT AATAGCTGTA CTAGAATCAA TTGAACGCCT ACCTCTCCAT
    TTGTATGACA CACCAGGATC CACGTATAAC CTCCAGATAC TTACAAGAAG ATTACGCTTT
    CGGTTGGAAC GTGCACCTGG TGAAACTTCA TTAATAGACC GGACAGGCAG GATGCTGAAG
    ATGGAGCCCT TAGCTACAGT TGAATCTCTG GAACAGTATC TGCTGAAAAT GGTGGCAAAG
    CAGTGGTACG ACTTTGACAG ATCCTCATTT GTTTTTGTTC GAAAATTAAG AGAAGGCCAA
    AATTTTATAT TCCGGCACCA ACATGACTTT GATGAGAATG GAATCATTTA CTGGATTGGA
    ACAAATGCAA AAACTGCGTA TGAATGGGTA AATCCTGCAG CTTATGGACT CGTAGTCGTC
    ACATCATCAG AAGGAAGGAA TCTGCCCTAT GGCCGCTTGG AGGACATACT AAGTCGAGAT
    AATTCAGCTT TAAATTGTCA CAGCAATGAT GATAAAAATG CCTGGTTTGC CATAGATCTG
    GGTGTCTGGG TAATACCTTC AGCATACACA CTTCGTCATG CTCGTGGCTA TGGAAGGTCT
    GCACTGAGAA ATTGGGTTTT CCAGGTATCC AAAGATGGAC AGAACTGGAC TTCTTTGTAT
    ACCCATGTTG ATGACTGCAG TCTCAATGAA CCAGGGTCAA CTGCAACATG GCCCCTTGAC
    CCAGCGAAGG ATGAAAAGCA AGGGTGGCGG CATGTCAGGC TGAAGCAGAT GGGGAAGAAC
    GCCAGTGGAC AGACGCACTA CCTCTCGCTC TCCGGCTTTG AACTCTATGG CACTGTGAAT
    GGCGTGTGCG AAGACCAACT AGGGAAAGCA GCTAAAGAAG CAGAAGCTAA TCTTAGAAGG
    CAGAGGCGCC TGGTGCGCTC TCAGGTCCTC AAGTACATGG TTCCAGGAGC ACGTGTTATC
    AGGGGCCTTG ATTGGAAGTG GCGAGATCAG GATGGCAGCC CGCAGGGAGA AGGCACAGTC
    ACAGGCGAGC TCCACAATGG CTGGATTGAT GTCACCTGGG ATGCTGGTGG CTCAAACTCT
    TACCGTATGG GCGCAGAAGG AAAATTTGAC CTCAAGCTTG CACCAGGGTA CGACCCTGAT
    ACAGTGGCAT CACCCAAACC TGTTTCATCC ACTGTTTCAG GCACAACGCA ATCATGGAGC
    AGCTTGGTGA AAAACAACTG TCCAGACAAG ACGTCTGCTG CTGCAGGCTC CTCAAGTAGA
    AAAGGGAGCA GCAGCTCTGT GTGTAGCGTG GCCAGTAGCA GCGACATCAG CTTGGCTTCG
    ACCAAAACGG AACGGAGATC AGAAATTGTA ATGGAACACA GTATAGTTTC AGGAGCTGAT
    GTCCATGAAC CAATTGTTGT TCTTTCATCT GCTGAAAACG TCCCTCAAAC AGAAGTAGGG
    TCATCTTCCA GTGCAAGCAC CAGCACCCTA ACAGCGGAAA CGGGAAGTGA AAATGCGGAA
    AGGAAGTTAG GCCCTGATAG TTCTGTTCGA GCTCCTGGGG AGTCTAGTGC GATATCCATG
    GGGATTGTTA GCGTTAGCTC TCCTGATGTT AGTTCGGTAT CTGAATTAAC CAATAAGGAA
    GCAGCTTCAC AACGACCCCT TAGCTCTTCG GCAAGTAACA GACTGTCGGT GAGCTCTCTG
    TTGGCAGCCG GGGCCCCTAT GAGCTCCAGT GCGAGTGTAC CTAACCTGTC CTCAAGAGAG
    ACATCTAGCT TGGAGAGCTT TGTCAGGAGA GTGGCAAACA TAGCACGGAC TAATGCCACC
    AACAACATGA ACCTAAGCCG AAGCAGCAGT GACAACAACA CTAACACTTT GGGGAGGAAC
    GTGATGAGCA CCGCAACTTC TCCACTTATG GGTGCTCAGA GTTTCCCTAA TTTGACCACA
    CCTGGTACTA CATCAACAGT GACAATGTCA ACATCGAGTG TTACGAGCAG CAGCAATGTA
    GCTACGGCAA CCACAGTTTT ATCAGTGGGT CAATCATTAA GTAATACTTT AACCACCAGC
    CTCACATCAA CCTCTAGTGA GAGTGACACA GGCCAGGAAG CAGAATACTC CTTATATGAT
    TTCCTCGACA GCTGCCGTGC CAGTACTCTC TTAGCTGAAC TAGATGATGA CGAGGATTTG
    CCTGAGCCTG ACGAGGAAGA CGATGAGAAT GAAGATGACA ATCAGGAGGA CCAAGAATAC
    GAGGAGGTTA TGATTCTGAG ACGTCCCTCC CTGCAACGCC GAGCTGGCTC TCGCTCTGAC
    GTAACACACC ATGTCGTCAC CTCCCAGCTC CCACAGGTCC CTTCTGGAGC AGGGAGCCGG
    CCTGTTGGGG AACAGGAAGA AGAAGAGTAT GAAACCAAAG GAGGCCGCCG CAGAGCCTGG
    GATGACGACT ATGTGCTAAA GCGCCAGTTT TCTGCACTGG TCCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CCAATGTCCA GCAGACAACT GACCTAGAAA TTCCTCCCCC AGGAACACCT
    CACTCAGAGC TCTTGGAGGA AGTTGAATGT ACTCCGTCAC CTCGCTTGGC TCTCACACTG
    AAAGTGACGG GGCTTGGAAC AACGCGGGAA GTTGAACTGC CACTTACCAA TTTCAGATCC
    ACCATCTTTT ACTATGTACA AAAACTGCTT CAACTGTCTT GTAATGGCAA TGTGAAGTCA
    GATAAACTTA GGCGTATTTG GGAGCCCACT TACACAATCA TGTATAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGGAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAACAG
    TACCTTGGCA CTGACGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATGCCGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTTAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGATTAAACC AGGGGGCTAT TTCTAGTCTT CAAAGTAGTG ATATTCTTAA TTTGACAAAA
    GAGCAGCCTC AGGCTAAAGC AGGCAACGGG CAGAGCCCCT GTGGAGTAGA GGACGTCCTT
    CAGCTTCTGC GGATTCTGTA TATAGTTGCA AGTGATCCTT ATTCAAGGAT ATCCCAAGAA
    GATGGCGATG AGCAGCCTCA GTTTACTTTT CCACCTGATG AATTTACAAG TAAAAAAATC
    ACCACAAAAA TTTTGCAGCA GATTGAGGAA CCGTTGGCAC TGGCCAGTGG GGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTGA TCCCATTCGA GACGAGGCAG
    CTTTATTTCA CCTGTACTGC ATTTGGTGCG TCCAGAGCAA TAGTGTGGCT ACAGAACAGG
    CGGGAAGCCA CTGTGGAGCG AACTCGGACC ACGAGCAGTG TACGGCGAGA TGATCCTGGG
    GAATTTAGAG TGGGTCGCCT CAAGCACGAA AGAGTGAAAG TTCCCCGTGG AGAGTCTCTG
    ATGGAGTGGG CCGAGAATGT CATGCAGATA CACGCAGATC GGAAGTCAGT TCTCGAGGTT
    GAATTTCTAG GAGAAGAAGG AACTGGCTTG GGACCCACTT TAGAGTTTTA TGCTCTTGTG
    GCGGCTGAGT TTCAGAGGAC TGACTTGGGG ACTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCATGTTGA TCTTGGAGGT GGATTGAAGC CTCCAGGATA CTATGTACAG
    CGGTCATGTG GACTGTTCAC AGCGCCATTC CCACAGGACA GTGATGAGCT TGAAAGGATC
    ACAAAACTCT TCCATTTCCT TGGGATTTTC TTGGCCAAAT GTATTCAGGA CAATAGACTG
    GTGGACTTGC CTATCTCTAA GCCTTTCTTT AAACTTATGT GCATGGGCGA CATTAAAAGC
    AATATGAGCA AACTAATCTA TGAGTCCCGC GGCGACAGAG ACTTGCACTG TACTGAAAGT
    CAGTCGGAAG CTTCTACAGA AGAAGGCCAT GACTCCCTCT CAGTGGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT CCTCGATCCC CCTAAGCCCA AACCCCCAGC CTGGTTTAAT
    GGGATTTTAA CTTGGGAAGA TTTTGAACTA GTAAATCCTC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT TAAGAGGCGC CAGATTCTAG GCAATAAAAG TCTTTCTGAA
    GATGAGAAGA ACACAAAGTT ACAGGAACTC GTGCTCAGGA ATCCATCAGG CTCGGGACCT
    CCACTGAGCA TCGAGGACTT AGGTTTAAAT TTCCAGTTTT GCCCATCTTC AAGAATATAT
    GGCTTTACAG CTGTGGATCT CAAGCCAAGT GGGGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTCTGTATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCTT TTCGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAGG AAGTCCAGAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GACATTATCA ATTACACGGA ACCTAAGCTT GGCTATACAC GTGACAGTCC TGGCTTCCTG
    CGGTTTGTGA GGGTTTTATG TGGCATGTCC TCAGATGAAA GGAAAGCATT CCTGCAGTTT
    ACCACTGGCT GTTCAACTCT GCCCCCAGGT GGACTGGCTA ACCTGCATCC GAGGCTCACA
    GTTGTACGAA AGGTTGATGC TACTGATGCG AGCTATCCAT CGGTCAACAC CTGTGTCCAC
    TATCTTAAGT TACCTGAGTA TTCTTCTGAG GAGATCATGA GAGAGCGCTT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242351.1
    CDS357..8186
    Translation

    Target ORF information:

    RefSeq Version XM_011244049.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain containing 1 (Hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244049.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGACCAGCA
    GTTGCATTAA TTCGAAAATT AATAGCTGTA CTAGAATCAA TTGAACGCCT ACCTCTCCAT
    TTGTATGACA CACCAGGATC CACGTATAAC CTCCAGATAC TTACAAGAAG ATTACGCTTT
    CGGTTGGAAC GTGCACCTGG TGAAACTTCA TTAATAGACC GGACAGGCAG GATGCTGAAG
    ATGGAGCCCT TAGCTACAGT TGAATCTCTG GAACAGTATC TGCTGAAAAT GGTGGCAAAG
    CAGTGGTACG ACTTTGACAG ATCCTCATTT GTTTTTGTTC GAAAATTAAG AGAAGGCCAA
    AATTTTATAT TCCGGCACCA ACATGACTTT GATGAGAATG GAATCATTTA CTGGATTGGA
    ACAAATGCAA AAACTGCGTA TGAATGGGTA AATCCTGCAG CTTATGGACT CGTAGTCGTC
    ACATCATCAG AAGGAAGGAA TCTGCCCTAT GGCCGCTTGG AGGACATACT AAGTCGAGAT
    AATTCAGCTT TAAATTGTCA CAGCAATGAT GATAAAAATG CCTGGTTTGC CATAGATCTG
    GGTGTCTGGG TAATACCTTC AGCATACACA CTTCGTCATG CTCGTGGCTA TGGAAGGTCT
    GCACTGAGAA ATTGGGTTTT CCAGGTATCC AAAGATGGAC AGAACTGGAC TTCTTTGTAT
    ACCCATGTTG ATGACTGCAG TCTCAATGAA CCAGGGTCAA CTGCAACATG GCCCCTTGAC
    CCAGCGAAGG ATGAAAAGCA AGGGTGGCGG CATGTCAGGC TGAAGCAGAT GGGGAAGAAC
    GCCAGTGGAC AGACGCACTA CCTCTCGCTC TCCGGCTTTG AACTCTATGG CACTGTGAAT
    GGCGTGTGCG AAGACCAACT AGGGAAAGCA GCTAAAGAAG CAGAAGCTAA TCTTAGAAGG
    CAGAGGCGCC TGGTGCGCTC TCAGGTCCTC AAGTACATGG TTCCAGGAGC ACGTGTTATC
    AGGGGCCTTG ATTGGAAGTG GCGAGATCAG GATGGCAGCC CGCAGGGAGA AGGCACAGTC
    ACAGGCGAGC TCCACAATGG CTGGATTGAT GTCACCTGGG ATGCTGGTGG CTCAAACTCT
    TACCGTATGG GCGCAGAAGG AAAATTTGAC CTCAAGCTTG CACCAGGGTA CGACCCTGAT
    ACAGTGGCAT CACCCAAACC TGTTTCATCC ACTGTTTCAG GCACAACGCA ATCATGGAGC
    AGCTTGGTGA AAAACAACTG TCCAGACAAG ACGTCTGCTG CTGCAGGCTC CTCAAGTAGA
    AAAGGGAGCA GCAGCTCTGT GTGTAGCGTG GCCAGTAGCA GCGACATCAG CTTGGCTTCG
    ACCAAAACGG AACGGAGATC AGAAATTGTA ATGGAACACA GTATAGTTTC AGGAGCTGAT
    GTCCATGAAC CAATTGTTGT TCTTTCATCT GCTGAAAACG TCCCTCAAAC AGAAGTAGGG
    TCATCTTCCA GTGCAAGCAC CAGCACCCTA ACAGCGGAAA CGGGAAGTGA AAATGCGGAA
    AGGAAGTTAG GCCCTGATAG TTCTGTTCGA GCTCCTGGGG AGTCTAGTGC GATATCCATG
    GGGATTGTTA GCGTTAGCTC TCCTGATGTT AGTTCGGTAT CTGAATTAAC CAATAAGGAA
    GCAGCTTCAC AACGACCCCT TAGCTCTTCG GCAAGTAACA GACTGTCGGT GAGCTCTCTG
    TTGGCAGCCG GGGCCCCTAT GAGCTCCAGT GCGAGTGTAC CTAACCTGTC CTCAAGAGAG
    ACATCTAGCT TGGAGAGCTT TGTCAGGAGA GTGGCAAACA TAGCACGGAC TAATGCCACC
    AACAACATGA ACCTAAGCCG AAGCAGCAGT GACAACAACA CTAACACTTT GGGGAGGAAC
    GTGATGAGCA CCGCAACTTC TCCACTTATG GGTGCTCAGA GTTTCCCTAA TTTGACCACA
    CCTGGTACTA CATCAACAGT GACAATGTCA ACATCGAGTG TTACGAGCAG CAGCAATGTA
    GCTACGGCAA CCACAGTTTT ATCAGTGGGT CAATCATTAA GTAATACTTT AACCACCAGC
    CTCACATCAA CCTCTAGTGA GAGTGACACA GGCCAGGAAG CAGAATACTC CTTATATGAT
    TTCCTCGACA GCTGCCGTGC CAGTACTCTC TTAGCTGAAC TAGATGATGA CGAGGATTTG
    CCTGAGCCTG ACGAGGAAGA CGATGAGAAT GAAGATGACA ATCAGGAGGA CCAAGAATAC
    GAGGAGGTTA TGATTCTGAG ACGTCCCTCC CTGCAACGCC GAGCTGGCTC TCGCTCTGAC
    GTAACACACC ATGTCGTCAC CTCCCAGCTC CCACAGGTCC CTTCTGGAGC AGGGAGCCGG
    CCTGTTGGGG AACAGGAAGA AGAAGAGTAT GAAACCAAAG GAGGCCGCCG CAGAGCCTGG
    GATGACGACT ATGTGCTAAA GCGCCAGTTT TCTGCACTGG TCCCTGCTTT TGATCCTAGA
    CCTGGTCGTA CCAATGTCCA GCAGACAACT GACCTAGAAA TTCCTCCCCC AGGAACACCT
    CACTCAGAGC TCTTGGAGGA AGTTGAATGT ACTCCGTCAC CTCGCTTGGC TCTCACACTG
    AAAGTGACGG GGCTTGGAAC AACGCGGGAA GTTGAACTGC CACTTACCAA TTTCAGATCC
    ACCATCTTTT ACTATGTACA AAAACTGCTT CAACTGTCTT GTAATGGCAA TGTGAAGTCA
    GATAAACTTA GGCGTATTTG GGAGCCCACT TACACAATCA TGTATAGAGA AATGAAGGAT
    TCTGATAAAG AAAAGGAAAA TGGGAAAATG GGTTGCTGGT CTATAGAGCA TGTGGAACAG
    TACCTTGGCA CTGACGAATT ACCAAAGAAT GACTTGATAA CCTACCTGCA GAAGAATGCA
    GATGCCGCTT TCCTGCGCCA CTGGAAATTA ACTGGCACTA ATAAAAGTAT TAGGAAAAAC
    AGAAATTGTT CTCAGCTTAT AGCTGCATAT AAGGATTTTT GTGAGCATGG AACAAAGTCT
    GGATTAAACC AGGGGGCTAT TTCTAGTCTT CAAAGTAGTG ATATTCTTAA TTTGACAAAA
    GAGCAGCCTC AGGCTAAAGC AGGCAACGGG CAGAGCCCCT GTGGAGTAGA GGACGTCCTT
    CAGCTTCTGC GGATTCTGTA TATAGTTGCA AGTGATCCTT ATTCAAGGAT ATCCCAAGAA
    GATGGCGATG AGCAGCCTCA GTTTACTTTT CCACCTGATG AATTTACAAG TAAAAAAATC
    ACCACAAAAA TTTTGCAGCA GATTGAGGAA CCGTTGGCAC TGGCCAGTGG GGCTCTGCCA
    GACTGGTGTG AACAGTTAAC CAGCAAGTGT CCTTTCCTGA TCCCATTCGA GACGAGGCAG
    CTTTATTTCA CCTGTACTGC ATTTGGTGCG TCCAGAGCAA TAGTGTGGCT ACAGAACAGG
    CGGGAAGCCA CTGTGGAGCG AACTCGGACC ACGAGCAGTG TACGGCGAGA TGATCCTGGG
    GAATTTAGAG TGGGTCGCCT CAAGCACGAA AGAGTGAAAG TTCCCCGTGG AGAGTCTCTG
    ATGGAGTGGG CCGAGAATGT CATGCAGATA CACGCAGATC GGAAGTCAGT TCTCGAGGTT
    GAATTTCTAG GAGAAGAAGG AACTGGCTTG GGACCCACTT TAGAGTTTTA TGCTCTTGTG
    GCGGCTGAGT TTCAGAGGAC TGACTTGGGG ACTTGGCTTT GTGATGATAA TTTTCCAGAT
    GATGAATCTC GTCATGTTGA TCTTGGAGGT GGATTGAAGC CTCCAGGATA CTATGTACAG
    CGGTCATGTG GACTGTTCAC AGCGCCATTC CCACAGGACA GTGATGAGCT TGAAAGGATC
    ACAAAACTCT TCCATTTCCT TGGGATTTTC TTGGCCAAAT GTATTCAGGA CAATAGACTG
    GTGGACTTGC CTATCTCTAA GCCTTTCTTT AAACTTATGT GCATGGGCGA CATTAAAAGC
    AATATGAGCA AACTAATCTA TGAGTCCCGC GGCGACAGAG ACTTGCACTG TACTGAAAGT
    CAGTCGGAAG CTTCTACAGA AGAAGGCCAT GACTCCCTCT CAGTGGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT CCTCGATCCC CCTAAGCCCA AACCCCCAGC CTGGTTTAAT
    GGGATTTTAA CTTGGGAAGA TTTTGAACTA GTAAATCCTC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT TAAGAGGCGC CAGATTCTAG GCAATAAAAG TCTTTCTGAA
    GATGAGAAGA ACACAAAGTT ACAGGAACTC GTGCTCAGGA ATCCATCAGG CTCGGGACCT
    CCACTGAGCA TCGAGGACTT AGGTTTAAAT TTCCAGTTTT GCCCATCTTC AAGAATATAT
    GGCTTTACAG CTGTGGATCT CAAGCCAAGT GGGGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTCTGTATGC ATACGGGTAT TCAGAAACAG
    ATGGAAGCTT TTCGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAGG AAGTCCAGAT GATTCTTTGT GGAAATCAGT CACCATCCTG GGCAGCAGAG
    GACATTATCA ATTACACGGA ACCTAAGCTT GGCTATACAC GTGACAGTCC TGGCTTCCTG
    CGGTTTGTGA GGGTTTTATG TGGCATGTCC TCAGATGAAA GGAAAGCATT CCTGCAGTTT
    ACCACTGGCT GTTCAACTCT GCCCCCAGGT GGACTGGCTA ACCTGCATCC GAGGCTCACA
    GTTGTACGAA AGGTTGATGC TACTGATGCG AGCTATCCAT CGGTCAACAC CTGTGTCCAC
    TATCTTAAGT TACCTGAGTA TTCTTCTGAG GAGATCATGA GAGAGCGCTT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72601
    Clone ID Related Accession (Same CDS sequence) XM_011244050.2 , XM_011244050.3
    Accession Version XM_011244050.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGGA GCAGCAGCTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGCT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCG
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCGATATCC
    ATGGGGATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCGG TATCTGAATT AACCAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCT TCGGCAAGTA ACAGACTGTC GGTGAGCTCT
    CTGTTGGCAG CCGGGGCCCC TATGAGCTCC AGTGCGAGTG TACCTAACCT GTCCTCAAGA
    GAGACATCTA GCTTGGAGAG CTTTGTCAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTAAG CCGAAGCAGC AGTGACAACA ACACTAACAC TTTGGGGAGG
    AACGTGATGA GCACCGCAAC TTCTCCACTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCGA GTGTTACGAG CAGCAGCAAT
    GTAGCTACGG CAACCACAGT TTTATCAGTG GGTCAATCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGCCAGG AAGCAGAATA CTCCTTATAT
    GATTTCCTCG ACAGCTGCCG TGCCAGTACT CTCTTAGCTG AACTAGATGA TGACGAGGAT
    TTGCCTGAGC CTGACGAGGA AGACGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCCTCCCTG CAACGCCGAG CTGGCTCTCG CTCTGACGTA
    ACACACCATG TCGTCACCTC CCAGCTCCCA CAGGTCCCTT CTGGAGCAGG GAGCCGGCCT
    GTTGGGGAAC AGGAAGAAGA AGAGTATGAA ACCAAAGGAG GCCGCCGCAG AGCCTGGGAT
    GACGACTATG TGCTAAAGCG CCAGTTTTCT GCACTGGTCC CTGCTTTTGA TCCTAGACCT
    GGTCGTACCA ATGTCCAGCA GACAACTGAC CTAGAAATTC CTCCCCCAGG AACACCTCAC
    TCAGAGCTCT TGGAGGAAGT TGAATGTACT CCGTCACCTC GCTTGGCTCT CACACTGAAA
    GTGACGGGGC TTGGAACAAC GCGGGAAGTT GAACTGCCAC TTACCAATTT CAGATCCACC
    ATCTTTTACT ATGTACAAAA ACTGCTTCAA CTGTCTTGTA ATGGCAATGT GAAGTCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACTTAC ACAATCATGT ATAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG GAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ACGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAT
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTTATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGA
    TTAAACCAGG GGGCTATTTC TAGTCTTCAA AGTAGTGATA TTCTTAATTT GACAAAAGAG
    CAGCCTCAGG CTAAAGCAGG CAACGGGCAG AGCCCCTGTG GAGTAGAGGA CGTCCTTCAG
    CTTCTGCGGA TTCTGTATAT AGTTGCAAGT GATCCTTATT CAAGGATATC CCAAGAAGAT
    GGCGATGAGC AGCCTCAGTT TACTTTTCCA CCTGATGAAT TTACAAGTAA AAAAATCACC
    ACAAAAATTT TGCAGCAGAT TGAGGAACCG TTGGCACTGG CCAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTCCTGATCC CATTCGAGAC GAGGCAGCTT
    TATTTCACCT GTACTGCATT TGGTGCGTCC AGAGCAATAG TGTGGCTACA GAACAGGCGG
    GAAGCCACTG TGGAGCGAAC TCGGACCACG AGCAGTGTAC GGCGAGATGA TCCTGGGGAA
    TTTAGAGTGG GTCGCCTCAA GCACGAAAGA GTGAAAGTTC CCCGTGGAGA GTCTCTGATG
    GAGTGGGCCG AGAATGTCAT GCAGATACAC GCAGATCGGA AGTCAGTTCT CGAGGTTGAA
    TTTCTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACTTTAG AGTTTTATGC TCTTGTGGCG
    GCTGAGTTTC AGAGGACTGA CTTGGGGACT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ATGTTGATCT TGGAGGTGGA TTGAAGCCTC CAGGATACTA TGTACAGCGG
    TCATGTGGAC TGTTCACAGC GCCATTCCCA CAGGACAGTG ATGAGCTTGA AAGGATCACA
    AAACTCTTCC ATTTCCTTGG GATTTTCTTG GCCAAATGTA TTCAGGACAA TAGACTGGTG
    GACTTGCCTA TCTCTAAGCC TTTCTTTAAA CTTATGTGCA TGGGCGACAT TAAAAGCAAT
    ATGAGCAAAC TAATCTATGA GTCCCGCGGC GACAGAGACT TGCACTGTAC TGAAAGTCAG
    TCGGAAGCTT CTACAGAAGA AGGCCATGAC TCCCTCTCAG TGGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATCCT CGATCCCCCT AAGCCCAAAC CCCCAGCCTG GTTTAATGGG
    ATTTTAACTT GGGAAGATTT TGAACTAGTA AATCCTCACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATTAA GAGGCGCCAG ATTCTAGGCA ATAAAAGTCT TTCTGAAGAT
    GAGAAGAACA CAAAGTTACA GGAACTCGTG CTCAGGAATC CATCAGGCTC GGGACCTCCA
    CTGAGCATCG AGGACTTAGG TTTAAATTTC CAGTTTTGCC CATCTTCAAG AATATATGGC
    TTTACAGCTG TGGATCTCAA GCCAAGTGGG GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTC TGTATGCATA CGGGTATTCA GAAACAGATG
    GAAGCTTTTC GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAGGAAG TCCAGATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAC
    ATTATCAATT ACACGGAACC TAAGCTTGGC TATACACGTG ACAGTCCTGG CTTCCTGCGG
    TTTGTGAGGG TTTTATGTGG CATGTCCTCA GATGAAAGGA AAGCATTCCT GCAGTTTACC
    ACTGGCTGTT CAACTCTGCC CCCAGGTGGA CTGGCTAACC TGCATCCGAG GCTCACAGTT
    GTACGAAAGG TTGATGCTAC TGATGCGAGC TATCCATCGG TCAACACCTG TGTCCACTAT
    CTTAAGTTAC CTGAGTATTC TTCTGAGGAG ATCATGAGAG AGCGCTTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242352.1
    CDS357..8183
    Translation

    Target ORF information:

    RefSeq Version XM_011244050.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain containing 1 (Hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGGA GCAGCAGCTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGCT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCG
    GAAAGGAAGT TAGGCCCTGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCGATATCC
    ATGGGGATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCGG TATCTGAATT AACCAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCT TCGGCAAGTA ACAGACTGTC GGTGAGCTCT
    CTGTTGGCAG CCGGGGCCCC TATGAGCTCC AGTGCGAGTG TACCTAACCT GTCCTCAAGA
    GAGACATCTA GCTTGGAGAG CTTTGTCAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTAAG CCGAAGCAGC AGTGACAACA ACACTAACAC TTTGGGGAGG
    AACGTGATGA GCACCGCAAC TTCTCCACTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCAAC AGTGACAATG TCAACATCGA GTGTTACGAG CAGCAGCAAT
    GTAGCTACGG CAACCACAGT TTTATCAGTG GGTCAATCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGCCAGG AAGCAGAATA CTCCTTATAT
    GATTTCCTCG ACAGCTGCCG TGCCAGTACT CTCTTAGCTG AACTAGATGA TGACGAGGAT
    TTGCCTGAGC CTGACGAGGA AGACGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCCTCCCTG CAACGCCGAG CTGGCTCTCG CTCTGACGTA
    ACACACCATG TCGTCACCTC CCAGCTCCCA CAGGTCCCTT CTGGAGCAGG GAGCCGGCCT
    GTTGGGGAAC AGGAAGAAGA AGAGTATGAA ACCAAAGGAG GCCGCCGCAG AGCCTGGGAT
    GACGACTATG TGCTAAAGCG CCAGTTTTCT GCACTGGTCC CTGCTTTTGA TCCTAGACCT
    GGTCGTACCA ATGTCCAGCA GACAACTGAC CTAGAAATTC CTCCCCCAGG AACACCTCAC
    TCAGAGCTCT TGGAGGAAGT TGAATGTACT CCGTCACCTC GCTTGGCTCT CACACTGAAA
    GTGACGGGGC TTGGAACAAC GCGGGAAGTT GAACTGCCAC TTACCAATTT CAGATCCACC
    ATCTTTTACT ATGTACAAAA ACTGCTTCAA CTGTCTTGTA ATGGCAATGT GAAGTCAGAT
    AAACTTAGGC GTATTTGGGA GCCCACTTAC ACAATCATGT ATAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG GAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ACGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAT
    GCCGCTTTCC TGCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTTATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGA
    TTAAACCAGG GGGCTATTTC TAGTCTTCAA AGTAGTGATA TTCTTAATTT GACAAAAGAG
    CAGCCTCAGG CTAAAGCAGG CAACGGGCAG AGCCCCTGTG GAGTAGAGGA CGTCCTTCAG
    CTTCTGCGGA TTCTGTATAT AGTTGCAAGT GATCCTTATT CAAGGATATC CCAAGAAGAT
    GGCGATGAGC AGCCTCAGTT TACTTTTCCA CCTGATGAAT TTACAAGTAA AAAAATCACC
    ACAAAAATTT TGCAGCAGAT TGAGGAACCG TTGGCACTGG CCAGTGGGGC TCTGCCAGAC
    TGGTGTGAAC AGTTAACCAG CAAGTGTCCT TTCCTGATCC CATTCGAGAC GAGGCAGCTT
    TATTTCACCT GTACTGCATT TGGTGCGTCC AGAGCAATAG TGTGGCTACA GAACAGGCGG
    GAAGCCACTG TGGAGCGAAC TCGGACCACG AGCAGTGTAC GGCGAGATGA TCCTGGGGAA
    TTTAGAGTGG GTCGCCTCAA GCACGAAAGA GTGAAAGTTC CCCGTGGAGA GTCTCTGATG
    GAGTGGGCCG AGAATGTCAT GCAGATACAC GCAGATCGGA AGTCAGTTCT CGAGGTTGAA
    TTTCTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACTTTAG AGTTTTATGC TCTTGTGGCG
    GCTGAGTTTC AGAGGACTGA CTTGGGGACT TGGCTTTGTG ATGATAATTT TCCAGATGAT
    GAATCTCGTC ATGTTGATCT TGGAGGTGGA TTGAAGCCTC CAGGATACTA TGTACAGCGG
    TCATGTGGAC TGTTCACAGC GCCATTCCCA CAGGACAGTG ATGAGCTTGA AAGGATCACA
    AAACTCTTCC ATTTCCTTGG GATTTTCTTG GCCAAATGTA TTCAGGACAA TAGACTGGTG
    GACTTGCCTA TCTCTAAGCC TTTCTTTAAA CTTATGTGCA TGGGCGACAT TAAAAGCAAT
    ATGAGCAAAC TAATCTATGA GTCCCGCGGC GACAGAGACT TGCACTGTAC TGAAAGTCAG
    TCGGAAGCTT CTACAGAAGA AGGCCATGAC TCCCTCTCAG TGGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATCCT CGATCCCCCT AAGCCCAAAC CCCCAGCCTG GTTTAATGGG
    ATTTTAACTT GGGAAGATTT TGAACTAGTA AATCCTCACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATTAA GAGGCGCCAG ATTCTAGGCA ATAAAAGTCT TTCTGAAGAT
    GAGAAGAACA CAAAGTTACA GGAACTCGTG CTCAGGAATC CATCAGGCTC GGGACCTCCA
    CTGAGCATCG AGGACTTAGG TTTAAATTTC CAGTTTTGCC CATCTTCAAG AATATATGGC
    TTTACAGCTG TGGATCTCAA GCCAAGTGGG GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTC TGTATGCATA CGGGTATTCA GAAACAGATG
    GAAGCTTTTC GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAGGAAG TCCAGATGAT TCTTTGTGGA AATCAGTCAC CATCCTGGGC AGCAGAGGAC
    ATTATCAATT ACACGGAACC TAAGCTTGGC TATACACGTG ACAGTCCTGG CTTCCTGCGG
    TTTGTGAGGG TTTTATGTGG CATGTCCTCA GATGAAAGGA AAGCATTCCT GCAGTTTACC
    ACTGGCTGTT CAACTCTGCC CCCAGGTGGA CTGGCTAACC TGCATCCGAG GCTCACAGTT
    GTACGAAAGG TTGATGCTAC TGATGCGAGC TATCCATCGG TCAACACCTG TGTCCACTAT
    CTTAAGTTAC CTGAGTATTC TTCTGAGGAG ATCATGAGAG AGCGCTTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72602
    Clone ID Related Accession (Same CDS sequence) XM_011244051.3 , XM_011244051.2
    Accession Version XM_011244051.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244051.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCCT CCCTGCAACG CCGAGCTGGC TCTCGCTCTG ACGTAACACA CCATGTCGTC
    ACCTCCCAGC TCCCACAGGT CCCTTCTGGA GCAGGGAGCC GGCCTGTTGG GGAACAGGAA
    GAAGAAGAGT ATGAAACCAA AGGAGGCCGC CGCAGAGCCT GGGATGACGA CTATGTGCTA
    AAGCGCCAGT TTTCTGCACT GGTCCCTGCT TTTGATCCTA GACCTGGTCG TACCAATGTC
    CAGCAGACAA CTGACCTAGA AATTCCTCCC CCAGGAACAC CTCACTCAGA GCTCTTGGAG
    GAAGTTGAAT GTACTCCGTC ACCTCGCTTG GCTCTCACAC TGAAAGTGAC GGGGCTTGGA
    ACAACGCGGG AAGTTGAACT GCCACTTACC AATTTCAGAT CCACCATCTT TTACTATGTA
    CAAAAACTGC TTCAACTGTC TTGTAATGGC AATGTGAAGT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CTTACACAAT CATGTATAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGGAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGACGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGATGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTT
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGATTAAA CCAGGGGGCT
    ATTTCTAGTC TTCAAAGTAG TGATATTCTT AATTTGACAA AAGAGCAGCC TCAGGCTAAA
    GCAGGCAACG GGCAGAGCCC CTGTGGAGTA GAGGACGTCC TTCAGCTTCT GCGGATTCTG
    TATATAGTTG CAAGTGATCC TTATTCAAGG ATATCCCAAG AAGATGGCGA TGAGCAGCCT
    CAGTTTACTT TTCCACCTGA TGAATTTACA AGTAAAAAAA TCACCACAAA AATTTTGCAG
    CAGATTGAGG AACCGTTGGC ACTGGCCAGT GGGGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT GATCCCATTC GAGACGAGGC AGCTTTATTT CACCTGTACT
    GCATTTGGTG CGTCCAGAGC AATAGTGTGG CTACAGAACA GGCGGGAAGC CACTGTGGAG
    CGAACTCGGA CCACGAGCAG TGTACGGCGA GATGATCCTG GGGAATTTAG AGTGGGTCGC
    CTCAAGCACG AAAGAGTGAA AGTTCCCCGT GGAGAGTCTC TGATGGAGTG GGCCGAGAAT
    GTCATGCAGA TACACGCAGA TCGGAAGTCA GTTCTCGAGG TTGAATTTCT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC TTTAGAGTTT TATGCTCTTG TGGCGGCTGA GTTTCAGAGG
    ACTGACTTGG GGACTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCATGTT
    GATCTTGGAG GTGGATTGAA GCCTCCAGGA TACTATGTAC AGCGGTCATG TGGACTGTTC
    ACAGCGCCAT TCCCACAGGA CAGTGATGAG CTTGAAAGGA TCACAAAACT CTTCCATTTC
    CTTGGGATTT TCTTGGCCAA ATGTATTCAG GACAATAGAC TGGTGGACTT GCCTATCTCT
    AAGCCTTTCT TTAAACTTAT GTGCATGGGC GACATTAAAA GCAATATGAG CAAACTAATC
    TATGAGTCCC GCGGCGACAG AGACTTGCAC TGTACTGAAA GTCAGTCGGA AGCTTCTACA
    GAAGAAGGCC ATGACTCCCT CTCAGTGGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATCCTCGATC CCCCTAAGCC CAAACCCCCA GCCTGGTTTA ATGGGATTTT AACTTGGGAA
    GATTTTGAAC TAGTAAATCC TCACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATTAAGAGGC GCCAGATTCT AGGCAATAAA AGTCTTTCTG AAGATGAGAA GAACACAAAG
    TTACAGGAAC TCGTGCTCAG GAATCCATCA GGCTCGGGAC CTCCACTGAG CATCGAGGAC
    TTAGGTTTAA ATTTCCAGTT TTGCCCATCT TCAAGAATAT ATGGCTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGGGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTCTGTAT GCATACGGGT ATTCAGAAAC AGATGGAAGC TTTTCGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA GGAAGTCCAG
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGACATTAT CAATTACACG
    GAACCTAAGC TTGGCTATAC ACGTGACAGT CCTGGCTTCC TGCGGTTTGT GAGGGTTTTA
    TGTGGCATGT CCTCAGATGA AAGGAAAGCA TTCCTGCAGT TTACCACTGG CTGTTCAACT
    CTGCCCCCAG GTGGACTGGC TAACCTGCAT CCGAGGCTCA CAGTTGTACG AAAGGTTGAT
    GCTACTGATG CGAGCTATCC ATCGGTCAAC ACCTGTGTCC ACTATCTTAA GTTACCTGAG
    TATTCTTCTG AGGAGATCAT GAGAGAGCGC TTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011242353.1
    CDS357..8048
    Translation

    Target ORF information:

    RefSeq Version XM_011244051.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain containing 1 (Hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011244051.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TAGATCCTGA TACGTTGCTG GAATGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAGCAG CTATGCATGC TGCTTTTGAT GTCTGACAAT
    GTGGATCGCT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TGCCAGCTCT CTGCAAAATT
    TTCCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCCCGTGC CATAACATAC
    TACCTGGATG TTTCTGCAGA ATGCACCCGG AGGATAGTAG GGGTGGATGG AGCTATAAAA
    GCACTTTGTA ATCGCTTGGT GGTAGTTGAA CTTAACAACA GGACCAGCAG AGATTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATC TGCACGCGTG AGTCAGGGGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT CGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCTGTGGTA TCGAGACTCT GTGGCAAAAT GGAGCCTCAG
    GATTCCTCTT TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTAAAGCA TGAAGATCAT
    CAGGTTTCCG ATGGAGCTCT GCGGTGTTTT GCATCACTAG CTGACCGGTT TACTCGTCGT
    GGGGTAGACC CAGCTCCATT GGCCAAGCAT GGATTAACTG AGGAGCTGTT GTCTCGAATG
    GCAGCGGCTG GTGGTACTGT ATCAGGACCA TCTTCAGCAT GCAAACCAGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCTGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC GACAATTGTA
    AGTCTCCTCT CAACGCTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATCGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTGCGGA GATTGGATAG TTCTGGGGAG
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCGATTGACA CAGGAGCCTT TGAAGTCAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AACTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGGGGTGCT
    GATGTTAATA GAGGCCAAAG GTCCTCCTCA TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ACAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAGGAATG CAATGAACCC AGAGGGGATC CAGAAATGGC ACCGCTCTAC
    TTGAAGAGGT TGCTGCCAGT GTTTGCACAG ACATTTCAGC ACACTATGCT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATCCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTTT GTGATTCTGA CGTTGGTCAC AATTTGCCCA CAACACTAGT GGAAATCACT
    GCAACTGTCC TTGATCAAGA GGATGATGAT GATGGCCACT TGCTAGCTTT GCAGATCATA
    AGGGATCTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTTGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAACGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GATGCTAAGG AATTGCAGCA AGGTAAACCG
    TATCATTGGA GAGACTGGTC AATAATTAGA GGAAGGGACT GCTTATATAT TTGGAGTGAT
    GCTGCAGCTT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCTA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGGGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TACTGTCAGC ACCGGGACCC ACGAAACTGA CTGTAGGGAA CTGGTCATTA
    ACATGCTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAAGAAG ATTTGCCTGG TTTTGTATTT GAATCTAATA GAGGAACGAA ACATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA
    AAACTCAAAT CCAAGTTGGA GAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGACTTG
    TATGACGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GGGTGGTAGT GACACTCAGA
    AATATAGCAA CTCAGCTGGA GTCATCTTGG GAACTCCATA CGAATAGACA ATGTATCGAA
    GGTGAAAACA CTTGGCGAGA CTTAATGAAG ACAGCCTTAG AAAACCTAAT TGTACTGCTG
    AAGGATGAGA ACACGATCTC ACCGTATGAA ATGTGTAGTA GTGGTTTAGT CCAGGCACTT
    CTTACTGTTC TAAACAATAG TATAGATTTG GATATGAAGC AAGATTGTAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTT AGTGAAAGTG AAGATGATGA GAGCAGACCA
    GCAGTTGCAT TAATTCGAAA ATTAATAGCT GTACTAGAAT CAATTGAACG CCTACCTCTC
    CATTTGTATG ACACACCAGG ATCCACGTAT AACCTCCAGA TACTTACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT TCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGGCA
    AAGCAGTGGT ACGACTTTGA CAGATCCTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGC
    CAAAATTTTA TATTCCGGCA CCAACATGAC TTTGATGAGA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC GTATGAATGG GTAAATCCTG CAGCTTATGG ACTCGTAGTC
    GTCACATCAT CAGAAGGAAG GAATCTGCCC TATGGCCGCT TGGAGGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCACAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC TTCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC ATGGCCCCTT
    GACCCAGCGA AGGATGAAAA GCAAGGGTGG CGGCATGTCA GGCTGAAGCA GATGGGGAAG
    AACGCCAGTG GACAGACGCA CTACCTCTCG CTCTCCGGCT TTGAACTCTA TGGCACTGTG
    AATGGCGTGT GCGAAGACCA ACTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG CTCTCAGGTC CTCAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGGGGCC TTGATTGGAA GTGGCGAGAT CAGGATGGCA GCCCGCAGGG AGAAGGCACA
    GTCACAGGCG AGCTCCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGGAG CAGCAGCTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGCTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCGG AAAGGAAGTT AGGCCCTGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCGATATCCA TGGGGATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCGGT ATCTGAATTA ACCAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCTT CGGCAAGTAA CAGACTGTCG GTGAGCTCTC TGTTGGCAGC CGGGGCCCCT
    ATGAGCTCCA GTGCGAGTGT ACCTAACCTG TCCTCAAGAG AGACATCTAG CTTGGAGAGC
    TTTGTCAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTAAGC
    CGAAGCAGCA GTGACAACAA CACTAACACT TTGGGGAGGA ACGTGATGAG CACCGCAACT
    TCTCCACTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA
    GTGACAATGT CAACATCGAG TGTTACGAGC AGCAGCAATG TAGCTACGGC AACCACAGTT
    TTATCAGTGG GTCAATCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGCCAGGA AGCAGAATAC TCCTTATATG ATTTCCTCGA CAGCTGCCGT
    GCCAGTACTC TCTTAGCTGA ACTAGATGAT GACGAGGATT TGCCTGAGCC TGACGAGGAA
    GACGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCCT CCCTGCAACG CCGAGCTGGC TCTCGCTCTG ACGTAACACA CCATGTCGTC
    ACCTCCCAGC TCCCACAGGT CCCTTCTGGA GCAGGGAGCC GGCCTGTTGG GGAACAGGAA
    GAAGAAGAGT ATGAAACCAA AGGAGGCCGC CGCAGAGCCT GGGATGACGA CTATGTGCTA
    AAGCGCCAGT TTTCTGCACT GGTCCCTGCT TTTGATCCTA GACCTGGTCG TACCAATGTC
    CAGCAGACAA CTGACCTAGA AATTCCTCCC CCAGGAACAC CTCACTCAGA GCTCTTGGAG
    GAAGTTGAAT GTACTCCGTC ACCTCGCTTG GCTCTCACAC TGAAAGTGAC GGGGCTTGGA
    ACAACGCGGG AAGTTGAACT GCCACTTACC AATTTCAGAT CCACCATCTT TTACTATGTA
    CAAAAACTGC TTCAACTGTC TTGTAATGGC AATGTGAAGT CAGATAAACT TAGGCGTATT
    TGGGAGCCCA CTTACACAAT CATGTATAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGGAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGACGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGATGCCGC TTTCCTGCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTT
    ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGATTAAA CCAGGGGGCT
    ATTTCTAGTC TTCAAAGTAG TGATATTCTT AATTTGACAA AAGAGCAGCC TCAGGCTAAA
    GCAGGCAACG GGCAGAGCCC CTGTGGAGTA GAGGACGTCC TTCAGCTTCT GCGGATTCTG
    TATATAGTTG CAAGTGATCC TTATTCAAGG ATATCCCAAG AAGATGGCGA TGAGCAGCCT
    CAGTTTACTT TTCCACCTGA TGAATTTACA AGTAAAAAAA TCACCACAAA AATTTTGCAG
    CAGATTGAGG AACCGTTGGC ACTGGCCAGT GGGGCTCTGC CAGACTGGTG TGAACAGTTA
    ACCAGCAAGT GTCCTTTCCT GATCCCATTC GAGACGAGGC AGCTTTATTT CACCTGTACT
    GCATTTGGTG CGTCCAGAGC AATAGTGTGG CTACAGAACA GGCGGGAAGC CACTGTGGAG
    CGAACTCGGA CCACGAGCAG TGTACGGCGA GATGATCCTG GGGAATTTAG AGTGGGTCGC
    CTCAAGCACG AAAGAGTGAA AGTTCCCCGT GGAGAGTCTC TGATGGAGTG GGCCGAGAAT
    GTCATGCAGA TACACGCAGA TCGGAAGTCA GTTCTCGAGG TTGAATTTCT AGGAGAAGAA
    GGAACTGGCT TGGGACCCAC TTTAGAGTTT TATGCTCTTG TGGCGGCTGA GTTTCAGAGG
    ACTGACTTGG GGACTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCATGTT
    GATCTTGGAG GTGGATTGAA GCCTCCAGGA TACTATGTAC AGCGGTCATG TGGACTGTTC
    ACAGCGCCAT TCCCACAGGA CAGTGATGAG CTTGAAAGGA TCACAAAACT CTTCCATTTC
    CTTGGGATTT TCTTGGCCAA ATGTATTCAG GACAATAGAC TGGTGGACTT GCCTATCTCT
    AAGCCTTTCT TTAAACTTAT GTGCATGGGC GACATTAAAA GCAATATGAG CAAACTAATC
    TATGAGTCCC GCGGCGACAG AGACTTGCAC TGTACTGAAA GTCAGTCGGA AGCTTCTACA
    GAAGAAGGCC ATGACTCCCT CTCAGTGGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATCCTCGATC CCCCTAAGCC CAAACCCCCA GCCTGGTTTA ATGGGATTTT AACTTGGGAA
    GATTTTGAAC TAGTAAATCC TCACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATTAAGAGGC GCCAGATTCT AGGCAATAAA AGTCTTTCTG AAGATGAGAA GAACACAAAG
    TTACAGGAAC TCGTGCTCAG GAATCCATCA GGCTCGGGAC CTCCACTGAG CATCGAGGAC
    TTAGGTTTAA ATTTCCAGTT TTGCCCATCT TCAAGAATAT ATGGCTTTAC AGCTGTGGAT
    CTCAAGCCAA GTGGGGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTCTGTAT GCATACGGGT ATTCAGAAAC AGATGGAAGC TTTTCGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA GGAAGTCCAG
    ATGATTCTTT GTGGAAATCA GTCACCATCC TGGGCAGCAG AGGACATTAT CAATTACACG
    GAACCTAAGC TTGGCTATAC ACGTGACAGT CCTGGCTTCC TGCGGTTTGT GAGGGTTTTA
    TGTGGCATGT CCTCAGATGA AAGGAAAGCA TTCCTGCAGT TTACCACTGG CTGTTCAACT
    CTGCCCCCAG GTGGACTGGC TAACCTGCAT CCGAGGCTCA CAGTTGTACG AAAGGTTGAT
    GCTACTGATG CGAGCTATCC ATCGGTCAAC ACCTGTGTCC ACTATCTTAA GTTACCTGAG
    TATTCTTCTG AGGAGATCAT GAGAGAGCGC TTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.