Spic cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spic
Entrez Gene ID	20728
Full Name	Spi-C transcription factor (Spi-1/PU.1 related)
Synonyms	AU019198,C76795,Prf,Spi-C
General protein information	Preferred Names Spi-C transcription factor (Spi-1/PU.1 related) Names transcription factor Spi-C pu.1-related factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_011461.3	NP_035591.3	transcription factor Spi-C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPIC	XP_001154302.1
Rattus norvegicus (Norway rat)	Spic	NP_001101550.1
Danio rerio (zebrafish)	spic	NP_001004621.1
Mus musculus (house mouse)	Spic	NP_035591.3
Gallus gallus (chicken)	SPIC	XP_425488.3
Homo sapiens (human)	SPIC	NP_689536.1
	SPIC	XP_001084463.1
Canis lupus familiaris (dog)	SPIC	XP_854994.1
Bos taurus (cattle)	SPIC	NP_001069889.1
Xenopus tropicalis (tropical clawed frog)	spic	XP_002936885.1

Gene Ontology
Bibliography

Related articles in PubMed

The macrophage contribution to stress erythropoiesis: when less is enough.
Ulyanova T, Phelps SR, Papayannopoulou T
Blood128(13)1756-65(2016 09)

RAG-mediated DNA double-strand breaks activate a cell type-specific checkpoint to inhibit pre-B cell receptor signals.
Bednarski JJ, Pandey R, Schulte E, White LS, Chen BR, Sandoval GJ, Kohyama M, Haldar M, Nickless A, Trott A, Cheng G, Murphy KM, Bassing CH, Payton JE, Sleckman BP
The Journal of experimental medicine213(2)209-23(2016 Feb)

Articular cartilage endurance and resistance to osteoarthritic changes require transcription factor Erg.
Ohta Y, Okabe T, Larmour C, Di Rocco A, Maijenburg MW, Phillips A, Speck NA, Wakitani S, Nakamura T, Yamada Y, Enomoto-Iwamoto M, Pacifici M, Iwamoto M
Arthritis & rheumatology (Hoboken, N.J.)67(10)2679-90(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate a novel role for Spi-C as a negative regulator of B cell development and function.
Title: Identification of a negative regulatory role for spi-C in the murine B cell lineage.

Study reports that Spic regulated the development of F4/80(+)VCAM1(+) bone marrow macrophages (BMM) and that Spic expression in BMM and red pulp macrophages development was induced by heme, a metabolite of erythrocyte degradation.
Title: Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C.
Title: Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C.

Spi-C controls development of red pulp macrophages required for red blood cell recycling and iron homeostasis
Title: Role for Spi-C in the development of red pulp macrophages and splenic iron homeostasis.

data indicate that ectopic expression of Spi-C can impair B-cell development and function by affecting genes associated with BCR signaling
Title: Transgenic expression of Spi-C impairs B-cell development and function by affecting genes associated with BCR signaling.

The following Spic gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spic cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08199	NM_011461.3 Latest version!	Mus musculus Spi-C transcription factor (Spi-1/PU.1 related) (Spic), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spic ( NM_011461.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035591.3 Mus musculus Spi-C transcription factor (Spi-1/PU.1 related) (Spic), mRNA.

Price & Availability↑

CloneID	OMu08199
Clone ID Related Accession (Same CDS sequence)	NM_011461.3
Accession Version	NM_011461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Mus musculus(house mouse)
Product	transcription factor Spi-C
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK137522.1 and BC064038.1. On Jan 18, 2008 this sequence version replaced NM_011461.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064038.1, AK156892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGACTTGTT GTATTGATCA AGACAGTTTG GGGCAAACAT TTCAAGACGC CATTGACATA 
TTGATCCAAC AATCAGCTGG AGAAAGTCAA TACTCCTCAG AAAATAGAAA TTACATGGCT 
ATCATTAACC CTTATCCTCA CGTCAGAGGC AACGCTAACT ACTATGGAAT GTCACCCACA 
GAGAACCCCC TCTATGACTG GAGAGGTGTA ACAAATGGTT CTGCAGACCT GTATTTGGAA 
GGAGGCTTCC ACCAATCCGT ACAGAACATA GCTGAAAGCC AGCTGGTACA ACCCCCCTTC 
TTCCAGCAAA AGGGAGGAAG AGGCAGGAGA AAGCTTCGGC TGTTTGAATA CCTTTTCGAA 
TCCCTGTGCA ATTCAGAGAT GGTGTCTTGT ATTCAGTGGG TTGACAAAGC CAGAGGCATC 
TTTCAGTTTA TATCAAAAAA CAAAGAAACA CTCGCTGAAC TCTGGGGGCA GAGAAAAGGC 
AACCGGAAAC CCATGACTTA CCAGAAGATG GCCAGAGCCC TGAGGAATTA TGCCAGAACT 
GGGGAGATCA TAAAAATCCG GAGAAAGCTG ACCTACCAGT TCAGCGAGGC TGTTCTCCAA 
AGACTTGCTC CAGCTAACTA CCTGGGAAAA GACCTCTTCT ACCCTCAGTA TGGCCAACCT 
GATCAGGGCT ACCTCAGTTT AAACCACTGG AATGCAAATC ACTATGCTCA CGGCAGTTAC 
CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035591.3
CDS	143..871
Translation	MTCCIDQDSLGQTFQDAIDILIQQSAGESQYSSENRNYMAIINPYPHVRGNANYYGMSPTENPLYDWRGVTNGSADLYLEGGFHQSVQNIAESQLVQPPFFQQKGGRGRRKLRLFEYLFESLCNSEMVSCIQWVDKARGIFQFISKNKETLAELWGQRKGNRKPMTYQKMARALRNYARTGEIIKIRRKLTYQFSEAVLQRLAPANYLGKDLFYPQYGQPDQGYLSLNHWNANHYAHGSYQS

Target ORF information:

RefSeq Version	NM_011461.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Spi-C transcription factor (Spi-1/PU.1 related) (Spic), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011461.3

ORF Insert Sequence:

ATGACTTGTT GTATTGATCA AGACAGTTTG GGGCAAACAT TTCAAGACGC CATTGACATA 
TTGATCCAAC AATCAGCTGG AGAAAGTCAA TACTCCTCAG AAAATAGAAA TTACATGGCT 
ATCATTAACC CTTATCCTCA CGTCAGAGGC AACGCTAACT ACTATGGAAT GTCACCCACA 
GAGAACCCCC TCTATGACTG GAGAGGTGTA ACAAATGGTT CTGCAGACCT GTATTTGGAA 
GGAGGCTTCC ACCAATCCGT ACAGAACATA GCTGAAAGCC AGCTGGTACA ACCCCCCTTC 
TTCCAGCAAA AGGGAGGAAG AGGCAGGAGA AAGCTTCGGC TGTTTGAATA CCTTTTCGAA 
TCCCTGTGCA ATTCAGAGAT GGTGTCTTGT ATTCAGTGGG TTGACAAAGC CAGAGGCATC 
TTTCAGTTTA TATCAAAAAA CAAAGAAACA CTCGCTGAAC TCTGGGGGCA GAGAAAAGGC 
AACCGGAAAC CCATGACTTA CCAGAAGATG GCCAGAGCCC TGAGGAATTA TGCCAGAACT 
GGGGAGATCA TAAAAATCCG GAGAAAGCTG ACCTACCAGT TCAGCGAGGC TGTTCTCCAA 
AGACTTGCTC CAGCTAACTA CCTGGGAAAA GACCTCTTCT ACCCTCAGTA TGGCCAACCT 
GATCAGGGCT ACCTCAGTTT AAACCACTGG AATGCAAATC ACTATGCTCA CGGCAGTTAC 
CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The macrophage contribution to stress erythropoiesis: when less is enough.
Blood128(13)1756-65(2016 09)
Ulyanova T,Phelps SR,Papayannopoulou T

RAG-mediated DNA double-strand breaks activate a cell type-specific checkpoint to inhibit pre-B cell receptor signals.
The Journal of experimental medicine213(2)209-23(2016 Feb)
Bednarski JJ,Pandey R,Schulte E,White LS,Chen BR,Sandoval GJ,Kohyama M,Haldar M,Nickless A,Trott A,Cheng G,Murphy KM,Bassing CH,Payton JE,Sleckman BP

Articular cartilage endurance and resistance to osteoarthritic changes require transcription factor Erg.
Arthritis & rheumatology (Hoboken, N.J.)67(10)2679-90(2015 Oct)
Ohta Y,Okabe T,Larmour C,Di Rocco A,Maijenburg MW,Phillips A,Speck NA,Wakitani S,Nakamura T,Yamada Y,Enomoto-Iwamoto M,Pacifici M,Iwamoto M

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Spic cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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