Sphk1 cDNA ORF clone, Mus musculus(house mouse)

The following Sphk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sphk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu45872 XM_006532674.3
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu16548 NM_001172475.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu16701 NM_011451.3
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $188.30
$269.00
OMu16548 XM_006532671.3
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu16548 XM_006532670.3
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu16548 XM_006532672.3
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu16499 NM_025367.7
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu16499 NM_001372484.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu16499 NM_001172473.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00
OMu16499 NM_001172472.2
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu16499 NM_001372486.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu16548 NM_001372489.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu16548 NM_001372488.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu45872 NM_001372487.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu45872 NM_001372485.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu16499 XM_006532673.3
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00
OMu45871 XM_006532669.4
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu77820 XM_017314398.1
Latest version!
Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu16499 NM_001172472.1 Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00
OMu16499 NM_025367.6 Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00
OMu45871 XM_006532669.3 Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu16499 XM_006532673.2 Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $188.30
$269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu45872
    Clone ID Related Accession (Same CDS sequence) XM_006532674.3 , NM_001372485.1 , NM_001372487.1
    Accession Version XM_006532674.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532674.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCCGGTG ATGGTCTGAT GCATGAGGTG GTGAATGGGC TAATGGAACG GCCAGACTGG 
    GAGACTGCCA TCCAGAAACC CCTGTGTAGC CTCCCTGGAG GCTCCGGCAA TGCGCTGGCA
    GCTTCTGTGA ACCACTATGC TGGGTACGAG CAGGTGACTA ATGAAGACCT GCTCATCAAC
    TGCACACTGC TGTTGTGCCG CCGGCGCCTG TCACCCATGA ACCTGCTGTC CCTGCACACT
    GCTTCTGGGC TGCGGCTCTA TTCTGTGCTC AGTCTGTCCT GGGGCTTTGT TGCTGACGTG
    GACCTCGAGA GTGAGAAGTA CAGGCGCTTG GGGGAGATTC GTTTCACAGT GGGCACCTTC
    TTTCGCCTAG CAAGCCTGCG CATCTACCAA GGCCAACTGG CCTACCTTCC TGTAGGAACT
    GTGGCCTCTA AGAGACCCGC CTCTACACTG GTGCAGAAGG GCCCCGTCGA CACACACCTT
    GTTCCTCTGG AGGAGCCAGT GCCTTCTCAT TGGACTGTGG TACCAGAACA GGACTTCGTC
    CTGGTGCTGG TGCTGCTACA CACCCACCTG AGCTCCGAGC TGTTTGCAGC ACCCATGGGC
    CGCTGTGAGG CTGGTGTTAT GCATCTGTTC TACGTACGTG CGGGGGTGTC AAGGGCTGCG
    CTGCTGCGCC TCTTCCTGGC CATGCAGAAG GGCAAGCATA TGGAACTTGA CTGTCCATAC
    CTGGTTCATG TGCCCGTGGT TGCTTTCCGC CTGGAGCCCA GGAGCCAGAG GGGCGTGTTT
    TCTGTGGATG GAGAGCTGAT GGTATGTGAA GCTGTGCAGG GCCAAGTGCA CCCAAACTAC
    CTTTGGATGG TCTGTGGCAG CAGAGATGCC CCATCCGGCC GGGACTCCCG GCGGGGGCCA
    CCTCCAGAAG AACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532737.1
    CDS1833..2750
    Translation

    Target ORF information:

    RefSeq Version XM_006532674.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532674.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCCGGTG ATGGTCTGAT GCATGAGGTG GTGAATGGGC TAATGGAACG GCCAGACTGG 
    GAGACTGCCA TCCAGAAACC CCTGTGTAGC CTCCCTGGAG GCTCCGGCAA TGCGCTGGCA
    GCTTCTGTGA ACCACTATGC TGGGTACGAG CAGGTGACTA ATGAAGACCT GCTCATCAAC
    TGCACACTGC TGTTGTGCCG CCGGCGCCTG TCACCCATGA ACCTGCTGTC CCTGCACACT
    GCTTCTGGGC TGCGGCTCTA TTCTGTGCTC AGTCTGTCCT GGGGCTTTGT TGCTGACGTG
    GACCTCGAGA GTGAGAAGTA CAGGCGCTTG GGGGAGATTC GTTTCACAGT GGGCACCTTC
    TTTCGCCTAG CAAGCCTGCG CATCTACCAA GGCCAACTGG CCTACCTTCC TGTAGGAACT
    GTGGCCTCTA AGAGACCCGC CTCTACACTG GTGCAGAAGG GCCCCGTCGA CACACACCTT
    GTTCCTCTGG AGGAGCCAGT GCCTTCTCAT TGGACTGTGG TACCAGAACA GGACTTCGTC
    CTGGTGCTGG TGCTGCTACA CACCCACCTG AGCTCCGAGC TGTTTGCAGC ACCCATGGGC
    CGCTGTGAGG CTGGTGTTAT GCATCTGTTC TACGTACGTG CGGGGGTGTC AAGGGCTGCG
    CTGCTGCGCC TCTTCCTGGC CATGCAGAAG GGCAAGCATA TGGAACTTGA CTGTCCATAC
    CTGGTTCATG TGCCCGTGGT TGCTTTCCGC CTGGAGCCCA GGAGCCAGAG GGGCGTGTTT
    TCTGTGGATG GAGAGCTGAT GGTATGTGAA GCTGTGCAGG GCCAAGTGCA CCCAAACTAC
    CTTTGGATGG TCTGTGGCAG CAGAGATGCC CCATCCGGCC GGGACTCCCG GCGGGGGCCA
    CCTCCAGAAG AACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16548
    Clone ID Related Accession (Same CDS sequence) XM_006532671.3 , XM_006532672.3 , NM_001172475.1 , XM_006532670.3 , NM_001372488.1 , NM_001372489.1
    Accession Version NM_001172475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. This isoform is also known as Sphk1a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF068748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849387 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165946.1
    CDS371..1516
    Translation

    Target ORF information:

    RefSeq Version NM_001172475.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001172475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16701
    Clone ID Related Accession (Same CDS sequence) NM_011451.3
    Accession Version NM_011451.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-22
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Feb 20, 2010 this sequence version replaced NM_011451.2. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1). This isoform is also known as Sphk1b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF068749.1, BI648186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTGGTGGT GTTGTGTTTT GTTTGTAGTA GAATGCCCTC GAGGACTGCT CCCACGGCCA 
    TGCAGAGTGC TGGTGCTGCT GAACCCCCAG GGTGGCAAGG GCAAGGCTCT GCAGCTCTTC
    CAGAGCCGTG TGCAGCCCTT CCTGGAGGAG GCAGAGATAA CCTTTAAACT GATACTCACC
    GAACGGAAGA ACCATGCCAG GGAGCTGGTG TGTGCAGAGG AGTTGGGTCA CTGGGACGCC
    CTGGCAGTCA TGTCCGGTGA TGGTCTGATG CATGAGGTGG TGAATGGGCT AATGGAACGG
    CCAGACTGGG AGACTGCCAT CCAGAAACCC CTGTGTAGCC TCCCTGGAGG CTCCGGCAAT
    GCGCTGGCAG CTTCTGTGAA CCACTATGCT GGGTACGAGC AGGTGACTAA TGAAGACCTG
    CTCATCAACT GCACACTGCT GTTGTGCCGC CGGCGCCTGT CACCCATGAA CCTGCTGTCC
    CTGCACACTG CTTCTGGGCT GCGGCTCTAT TCTGTGCTCA GTCTGTCCTG GGGCTTTGTT
    GCTGACGTGG ACCTCGAGAG TGAGAAGTAC AGGCGCTTGG GGGAGATTCG TTTCACAGTG
    GGCACCTTCT TTCGCCTAGC AAGCCTGCGC ATCTACCAAG GCCAACTGGC CTACCTTCCT
    GTAGGAACTG TGGCCTCTAA GAGACCCGCC TCTACACTGG TGCAGAAGGG CCCCGTCGAC
    ACACACCTTG TTCCTCTGGA GGAGCCAGTG CCTTCTCATT GGACTGTGGT ACCAGAACAG
    GACTTCGTCC TGGTGCTGGT GCTGCTACAC ACCCACCTGA GCTCCGAGCT GTTTGCAGCA
    CCCATGGGCC GCTGTGAGGC TGGTGTTATG CATCTGTTCT ACGTACGTGC GGGGGTGTCA
    AGGGCTGCGC TGCTGCGCCT CTTCCTGGCC ATGCAGAAGG GCAAGCATAT GGAACTTGAC
    TGTCCATACC TGGTTCATGT GCCCGTGGTT GCTTTCCGCC TGGAGCCCAG GAGCCAGAGG
    GGCGTGTTTT CTGTGGATGG AGAGCTGATG GTATGTGAAG CTGTGCAGGG CCAAGTGCAC
    CCAAACTACC TTTGGATGGT CTGTGGCAGC AGAGATGCCC CATCCGGCCG GGACTCCCGG
    CGGGGGCCAC CTCCAGAAGA ACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035581.1
    CDS82..1248
    Translation

    Target ORF information:

    RefSeq Version NM_011451.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011451.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTGGTGGT GTTGTGTTTT GTTTGTAGTA GAATGCCCTC GAGGACTGCT CCCACGGCCA 
    TGCAGAGTGC TGGTGCTGCT GAACCCCCAG GGTGGCAAGG GCAAGGCTCT GCAGCTCTTC
    CAGAGCCGTG TGCAGCCCTT CCTGGAGGAG GCAGAGATAA CCTTTAAACT GATACTCACC
    GAACGGAAGA ACCATGCCAG GGAGCTGGTG TGTGCAGAGG AGTTGGGTCA CTGGGACGCC
    CTGGCAGTCA TGTCCGGTGA TGGTCTGATG CATGAGGTGG TGAATGGGCT AATGGAACGG
    CCAGACTGGG AGACTGCCAT CCAGAAACCC CTGTGTAGCC TCCCTGGAGG CTCCGGCAAT
    GCGCTGGCAG CTTCTGTGAA CCACTATGCT GGGTACGAGC AGGTGACTAA TGAAGACCTG
    CTCATCAACT GCACACTGCT GTTGTGCCGC CGGCGCCTGT CACCCATGAA CCTGCTGTCC
    CTGCACACTG CTTCTGGGCT GCGGCTCTAT TCTGTGCTCA GTCTGTCCTG GGGCTTTGTT
    GCTGACGTGG ACCTCGAGAG TGAGAAGTAC AGGCGCTTGG GGGAGATTCG TTTCACAGTG
    GGCACCTTCT TTCGCCTAGC AAGCCTGCGC ATCTACCAAG GCCAACTGGC CTACCTTCCT
    GTAGGAACTG TGGCCTCTAA GAGACCCGCC TCTACACTGG TGCAGAAGGG CCCCGTCGAC
    ACACACCTTG TTCCTCTGGA GGAGCCAGTG CCTTCTCATT GGACTGTGGT ACCAGAACAG
    GACTTCGTCC TGGTGCTGGT GCTGCTACAC ACCCACCTGA GCTCCGAGCT GTTTGCAGCA
    CCCATGGGCC GCTGTGAGGC TGGTGTTATG CATCTGTTCT ACGTACGTGC GGGGGTGTCA
    AGGGCTGCGC TGCTGCGCCT CTTCCTGGCC ATGCAGAAGG GCAAGCATAT GGAACTTGAC
    TGTCCATACC TGGTTCATGT GCCCGTGGTT GCTTTCCGCC TGGAGCCCAG GAGCCAGAGG
    GGCGTGTTTT CTGTGGATGG AGAGCTGATG GTATGTGAAG CTGTGCAGGG CCAAGTGCAC
    CCAAACTACC TTTGGATGGT CTGTGGCAGC AGAGATGCCC CATCCGGCCG GGACTCCCGG
    CGGGGGCCAC CTCCAGAAGA ACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16548
    Clone ID Related Accession (Same CDS sequence) XM_006532671.3 , XM_006532672.3 , NM_001172475.1 , XM_006532670.3 , NM_001372488.1 , NM_001372489.1
    Accession Version XM_006532671.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532734.1
    CDS771..1916
    Translation

    Target ORF information:

    RefSeq Version XM_006532671.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532671.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16548
    Clone ID Related Accession (Same CDS sequence) XM_006532671.3 , XM_006532672.3 , NM_001172475.1 , XM_006532670.3 , NM_001372488.1 , NM_001372489.1
    Accession Version XM_006532672.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532735.1
    CDS561..1706
    Translation

    Target ORF information:

    RefSeq Version XM_006532672.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532672.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16548
    Clone ID Related Accession (Same CDS sequence) XM_006532671.3 , XM_006532672.3 , NM_001172475.1 , XM_006532670.3 , NM_001372488.1 , NM_001372489.1
    Accession Version XM_006532670.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532733.1
    CDS1457..2602
    Translation

    Target ORF information:

    RefSeq Version XM_006532670.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532670.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45871
    Clone ID Related Accession (Same CDS sequence) XM_006532669.4 , XM_006532669.3
    Accession Version XM_006532669.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTCGGG TCCGAATCCG GTGTCCCCTG GAACGGAATG TTCGTGGGGT GGGAAGCTCA 
    TTCTGGAAAG CTAGATCCCC CACCCACGGG GCTCTGGTGA TTCAGTCGCG AGCCAGGGGC
    TCTGGTTGGG CACTTTGTCC CACGTGCATG GAAGCCCAGG CAGAGAACCC CACCACTCTT
    TCCTCCTGTC CCTCAGATCT GGCCGCCCCG GGGAATGACG AGGGCGCTCA CACAGCCCAG
    GGAGGTGGAG AGGGCGAGCC CCACGGCCAG TCGCCAGACA CCCTCCTGGG CAACACCGAT
    AAGAAGCTGA ACGCAGGAGC CGCCGTTACC TCTAGCAGCG CCGGGGCAGC ACCGGTGGCC
    CCTTGTCAGC GGGAGCCCCG GGACCTGGCT ATGGAACCAG AATGCCCTCG AGGACTGCTC
    CCACGGCCAT GCAGAGTGCT GGTGCTGCTG AACCCCCAGG GTGGCAAGGG CAAGGCTCTG
    CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC CTGGAGGAGG CAGAGATAAC CTTTAAACTG
    ATACTCACCG AACGGAAGAA CCATGCCAGG GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC
    TGGGACGCCC TGGCAGTCAT GTCCGGTGAT GGTCTGATGC ATGAGGTGGT GAATGGGCTA
    ATGGAACGGC CAGACTGGGA GACTGCCATC CAGAAACCCC TGTGTAGCCT CCCTGGAGGC
    TCCGGCAATG CGCTGGCAGC TTCTGTGAAC CACTATGCTG GGTACGAGCA GGTGACTAAT
    GAAGACCTGC TCATCAACTG CACACTGCTG TTGTGCCGCC GGCGCCTGTC ACCCATGAAC
    CTGCTGTCCC TGCACACTGC TTCTGGGCTG CGGCTCTATT CTGTGCTCAG TCTGTCCTGG
    GGCTTTGTTG CTGACGTGGA CCTCGAGAGT GAGAAGTACA GGCGCTTGGG GGAGATTCGT
    TTCACAGTGG GCACCTTCTT TCGCCTAGCA AGCCTGCGCA TCTACCAAGG CCAACTGGCC
    TACCTTCCTG TAGGAACTGT GGCCTCTAAG AGACCCGCCT CTACACTGGT GCAGAAGGGC
    CCCGTCGACA CACACCTTGT TCCTCTGGAG GAGCCAGTGC CTTCTCATTG GACTGTGGTA
    CCAGAACAGG ACTTCGTCCT GGTGCTGGTG CTGCTACACA CCCACCTGAG CTCCGAGCTG
    TTTGCAGCAC CCATGGGCCG CTGTGAGGCT GGTGTTATGC ATCTGTTCTA CGTACGTGCG
    GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC TTCCTGGCCA TGCAGAAGGG CAAGCATATG
    GAACTTGACT GTCCATACCT GGTTCATGTG CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG
    AGCCAGAGGG GCGTGTTTTC TGTGGATGGA GAGCTGATGG TATGTGAAGC TGTGCAGGGC
    CAAGTGCACC CAAACTACCT TTGGATGGTC TGTGGCAGCA GAGATGCCCC ATCCGGCCGG
    GACTCCCGGC GGGGGCCACC TCCAGAAGAA CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532732.1
    CDS1668..3203
    Translation

    Target ORF information:

    RefSeq Version XM_006532669.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTCGGG TCCGAATCCG GTGTCCCCTG GAACGGAATG TTCGTGGGGT GGGAAGCTCA 
    TTCTGGAAAG CTAGATCCCC CACCCACGGG GCTCTGGTGA TTCAGTCGCG AGCCAGGGGC
    TCTGGTTGGG CACTTTGTCC CACGTGCATG GAAGCCCAGG CAGAGAACCC CACCACTCTT
    TCCTCCTGTC CCTCAGATCT GGCCGCCCCG GGGAATGACG AGGGCGCTCA CACAGCCCAG
    GGAGGTGGAG AGGGCGAGCC CCACGGCCAG TCGCCAGACA CCCTCCTGGG CAACACCGAT
    AAGAAGCTGA ACGCAGGAGC CGCCGTTACC TCTAGCAGCG CCGGGGCAGC ACCGGTGGCC
    CCTTGTCAGC GGGAGCCCCG GGACCTGGCT ATGGAACCAG AATGCCCTCG AGGACTGCTC
    CCACGGCCAT GCAGAGTGCT GGTGCTGCTG AACCCCCAGG GTGGCAAGGG CAAGGCTCTG
    CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC CTGGAGGAGG CAGAGATAAC CTTTAAACTG
    ATACTCACCG AACGGAAGAA CCATGCCAGG GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC
    TGGGACGCCC TGGCAGTCAT GTCCGGTGAT GGTCTGATGC ATGAGGTGGT GAATGGGCTA
    ATGGAACGGC CAGACTGGGA GACTGCCATC CAGAAACCCC TGTGTAGCCT CCCTGGAGGC
    TCCGGCAATG CGCTGGCAGC TTCTGTGAAC CACTATGCTG GGTACGAGCA GGTGACTAAT
    GAAGACCTGC TCATCAACTG CACACTGCTG TTGTGCCGCC GGCGCCTGTC ACCCATGAAC
    CTGCTGTCCC TGCACACTGC TTCTGGGCTG CGGCTCTATT CTGTGCTCAG TCTGTCCTGG
    GGCTTTGTTG CTGACGTGGA CCTCGAGAGT GAGAAGTACA GGCGCTTGGG GGAGATTCGT
    TTCACAGTGG GCACCTTCTT TCGCCTAGCA AGCCTGCGCA TCTACCAAGG CCAACTGGCC
    TACCTTCCTG TAGGAACTGT GGCCTCTAAG AGACCCGCCT CTACACTGGT GCAGAAGGGC
    CCCGTCGACA CACACCTTGT TCCTCTGGAG GAGCCAGTGC CTTCTCATTG GACTGTGGTA
    CCAGAACAGG ACTTCGTCCT GGTGCTGGTG CTGCTACACA CCCACCTGAG CTCCGAGCTG
    TTTGCAGCAC CCATGGGCCG CTGTGAGGCT GGTGTTATGC ATCTGTTCTA CGTACGTGCG
    GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC TTCCTGGCCA TGCAGAAGGG CAAGCATATG
    GAACTTGACT GTCCATACCT GGTTCATGTG CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG
    AGCCAGAGGG GCGTGTTTTC TGTGGATGGA GAGCTGATGG TATGTGAAGC TGTGCAGGGC
    CAAGTGCACC CAAACTACCT TTGGATGGTC TGTGGCAGCA GAGATGCCCC ATCCGGCCGG
    GACTCCCGGC GGGGGCCACC TCCAGAAGAA CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_001172472.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-04
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. Transcript Variant: This variant (4) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165943.1
    CDS443..1591
    Translation

    Target ORF information:

    RefSeq Version NM_001172472.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001172472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_025367.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced NM_025367.6. Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1427394.1, SRR7345562.2648278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079643.2
    CDS1113..2261
    Translation

    Target ORF information:

    RefSeq Version NM_025367.7
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025367.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_001172472.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced NM_001172472.1. Transcript Variant: This variant (4) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155332.1, SRR5189676.122982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165943.1
    CDS436..1584
    Translation

    Target ORF information:

    RefSeq Version NM_001172472.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001172472.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_001372486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced XM_006532673.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.761751.1, SRR7345562.1318706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359415.1
    CDS445..1593
    Translation

    Target ORF information:

    RefSeq Version NM_001372486.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_001372484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced XM_030245731.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2716910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359413.1
    CDS297..1445
    Translation

    Target ORF information:

    RefSeq Version NM_001372484.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_025367.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Feb 20, 2010 this sequence version replaced NM_025367.5. Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI413658.1, BM934157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079643.2
    CDS326..1474
    Translation

    Target ORF information:

    RefSeq Version NM_025367.6
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025367.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version NM_001172473.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8 and AK160900.1. Transcript Variant: This variant (3) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001165944.1
    CDS329..1477
    Translation

    Target ORF information:

    RefSeq Version NM_001172473.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001172473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16548
    Clone ID Related Accession (Same CDS sequence) XM_006532671.3 , XM_006532672.3 , NM_001172475.1 , XM_006532670.3 , NM_001372488.1 , NM_001372489.1
    Accession Version NM_001372488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced XM_006532670.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2059438.1, SRR7345562.1052043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359417.1
    CDS1113..2258
    Translation

    Target ORF information:

    RefSeq Version NM_001372488.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16548
    Clone ID Related Accession (Same CDS sequence) XM_006532671.3 , XM_006532672.3 , NM_001172475.1 , XM_006532670.3 , NM_001372488.1 , NM_001372489.1
    Accession Version NM_001372489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced XM_006532671.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.362542.1, SRR7345562.130819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359418.1
    CDS436..1581
    Translation

    Target ORF information:

    RefSeq Version NM_001372489.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG AATGCCCTCG AGGACTGCTC CCACGGCCAT GCAGAGTGCT GGTGCTGCTG 
    AACCCCCAGG GTGGCAAGGG CAAGGCTCTG CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC
    CTGGAGGAGG CAGAGATAAC CTTTAAACTG ATACTCACCG AACGGAAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC TGGGACGCCC TGGCAGTCAT GTCCGGTGAT
    GGTCTGATGC ATGAGGTGGT GAATGGGCTA ATGGAACGGC CAGACTGGGA GACTGCCATC
    CAGAAACCCC TGTGTAGCCT CCCTGGAGGC TCCGGCAATG CGCTGGCAGC TTCTGTGAAC
    CACTATGCTG GGTACGAGCA GGTGACTAAT GAAGACCTGC TCATCAACTG CACACTGCTG
    TTGTGCCGCC GGCGCCTGTC ACCCATGAAC CTGCTGTCCC TGCACACTGC TTCTGGGCTG
    CGGCTCTATT CTGTGCTCAG TCTGTCCTGG GGCTTTGTTG CTGACGTGGA CCTCGAGAGT
    GAGAAGTACA GGCGCTTGGG GGAGATTCGT TTCACAGTGG GCACCTTCTT TCGCCTAGCA
    AGCCTGCGCA TCTACCAAGG CCAACTGGCC TACCTTCCTG TAGGAACTGT GGCCTCTAAG
    AGACCCGCCT CTACACTGGT GCAGAAGGGC CCCGTCGACA CACACCTTGT TCCTCTGGAG
    GAGCCAGTGC CTTCTCATTG GACTGTGGTA CCAGAACAGG ACTTCGTCCT GGTGCTGGTG
    CTGCTACACA CCCACCTGAG CTCCGAGCTG TTTGCAGCAC CCATGGGCCG CTGTGAGGCT
    GGTGTTATGC ATCTGTTCTA CGTACGTGCG GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GAACTTGACT GTCCATACCT GGTTCATGTG
    CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG AGCCAGAGGG GCGTGTTTTC TGTGGATGGA
    GAGCTGATGG TATGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TGTGGCAGCA GAGATGCCCC ATCCGGCCGG GACTCCCGGC GGGGGCCACC TCCAGAAGAA
    CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45872
    Clone ID Related Accession (Same CDS sequence) XM_006532674.3 , NM_001372485.1 , NM_001372487.1
    Accession Version NM_001372485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced XM_030245732.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345558.133344.1, SRR7345560.872770.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCCGGTG ATGGTCTGAT GCATGAGGTG GTGAATGGGC TAATGGAACG GCCAGACTGG 
    GAGACTGCCA TCCAGAAACC CCTGTGTAGC CTCCCTGGAG GCTCCGGCAA TGCGCTGGCA
    GCTTCTGTGA ACCACTATGC TGGGTACGAG CAGGTGACTA ATGAAGACCT GCTCATCAAC
    TGCACACTGC TGTTGTGCCG CCGGCGCCTG TCACCCATGA ACCTGCTGTC CCTGCACACT
    GCTTCTGGGC TGCGGCTCTA TTCTGTGCTC AGTCTGTCCT GGGGCTTTGT TGCTGACGTG
    GACCTCGAGA GTGAGAAGTA CAGGCGCTTG GGGGAGATTC GTTTCACAGT GGGCACCTTC
    TTTCGCCTAG CAAGCCTGCG CATCTACCAA GGCCAACTGG CCTACCTTCC TGTAGGAACT
    GTGGCCTCTA AGAGACCCGC CTCTACACTG GTGCAGAAGG GCCCCGTCGA CACACACCTT
    GTTCCTCTGG AGGAGCCAGT GCCTTCTCAT TGGACTGTGG TACCAGAACA GGACTTCGTC
    CTGGTGCTGG TGCTGCTACA CACCCACCTG AGCTCCGAGC TGTTTGCAGC ACCCATGGGC
    CGCTGTGAGG CTGGTGTTAT GCATCTGTTC TACGTACGTG CGGGGGTGTC AAGGGCTGCG
    CTGCTGCGCC TCTTCCTGGC CATGCAGAAG GGCAAGCATA TGGAACTTGA CTGTCCATAC
    CTGGTTCATG TGCCCGTGGT TGCTTTCCGC CTGGAGCCCA GGAGCCAGAG GGGCGTGTTT
    TCTGTGGATG GAGAGCTGAT GGTATGTGAA GCTGTGCAGG GCCAAGTGCA CCCAAACTAC
    CTTTGGATGG TCTGTGGCAG CAGAGATGCC CCATCCGGCC GGGACTCCCG GCGGGGGCCA
    CCTCCAGAAG AACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359414.1
    CDS673..1590
    Translation

    Target ORF information:

    RefSeq Version NM_001372485.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCCGGTG ATGGTCTGAT GCATGAGGTG GTGAATGGGC TAATGGAACG GCCAGACTGG 
    GAGACTGCCA TCCAGAAACC CCTGTGTAGC CTCCCTGGAG GCTCCGGCAA TGCGCTGGCA
    GCTTCTGTGA ACCACTATGC TGGGTACGAG CAGGTGACTA ATGAAGACCT GCTCATCAAC
    TGCACACTGC TGTTGTGCCG CCGGCGCCTG TCACCCATGA ACCTGCTGTC CCTGCACACT
    GCTTCTGGGC TGCGGCTCTA TTCTGTGCTC AGTCTGTCCT GGGGCTTTGT TGCTGACGTG
    GACCTCGAGA GTGAGAAGTA CAGGCGCTTG GGGGAGATTC GTTTCACAGT GGGCACCTTC
    TTTCGCCTAG CAAGCCTGCG CATCTACCAA GGCCAACTGG CCTACCTTCC TGTAGGAACT
    GTGGCCTCTA AGAGACCCGC CTCTACACTG GTGCAGAAGG GCCCCGTCGA CACACACCTT
    GTTCCTCTGG AGGAGCCAGT GCCTTCTCAT TGGACTGTGG TACCAGAACA GGACTTCGTC
    CTGGTGCTGG TGCTGCTACA CACCCACCTG AGCTCCGAGC TGTTTGCAGC ACCCATGGGC
    CGCTGTGAGG CTGGTGTTAT GCATCTGTTC TACGTACGTG CGGGGGTGTC AAGGGCTGCG
    CTGCTGCGCC TCTTCCTGGC CATGCAGAAG GGCAAGCATA TGGAACTTGA CTGTCCATAC
    CTGGTTCATG TGCCCGTGGT TGCTTTCCGC CTGGAGCCCA GGAGCCAGAG GGGCGTGTTT
    TCTGTGGATG GAGAGCTGAT GGTATGTGAA GCTGTGCAGG GCCAAGTGCA CCCAAACTAC
    CTTTGGATGG TCTGTGGCAG CAGAGATGCC CCATCCGGCC GGGACTCCCG GCGGGGGCCA
    CCTCCAGAAG AACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45872
    Clone ID Related Accession (Same CDS sequence) XM_006532674.3 , NM_001372485.1 , NM_001372487.1
    Accession Version NM_001372487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645851.8. On Sep 3, 2019 this sequence version replaced XM_006532674.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.3553602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCCGGTG ATGGTCTGAT GCATGAGGTG GTGAATGGGC TAATGGAACG GCCAGACTGG 
    GAGACTGCCA TCCAGAAACC CCTGTGTAGC CTCCCTGGAG GCTCCGGCAA TGCGCTGGCA
    GCTTCTGTGA ACCACTATGC TGGGTACGAG CAGGTGACTA ATGAAGACCT GCTCATCAAC
    TGCACACTGC TGTTGTGCCG CCGGCGCCTG TCACCCATGA ACCTGCTGTC CCTGCACACT
    GCTTCTGGGC TGCGGCTCTA TTCTGTGCTC AGTCTGTCCT GGGGCTTTGT TGCTGACGTG
    GACCTCGAGA GTGAGAAGTA CAGGCGCTTG GGGGAGATTC GTTTCACAGT GGGCACCTTC
    TTTCGCCTAG CAAGCCTGCG CATCTACCAA GGCCAACTGG CCTACCTTCC TGTAGGAACT
    GTGGCCTCTA AGAGACCCGC CTCTACACTG GTGCAGAAGG GCCCCGTCGA CACACACCTT
    GTTCCTCTGG AGGAGCCAGT GCCTTCTCAT TGGACTGTGG TACCAGAACA GGACTTCGTC
    CTGGTGCTGG TGCTGCTACA CACCCACCTG AGCTCCGAGC TGTTTGCAGC ACCCATGGGC
    CGCTGTGAGG CTGGTGTTAT GCATCTGTTC TACGTACGTG CGGGGGTGTC AAGGGCTGCG
    CTGCTGCGCC TCTTCCTGGC CATGCAGAAG GGCAAGCATA TGGAACTTGA CTGTCCATAC
    CTGGTTCATG TGCCCGTGGT TGCTTTCCGC CTGGAGCCCA GGAGCCAGAG GGGCGTGTTT
    TCTGTGGATG GAGAGCTGAT GGTATGTGAA GCTGTGCAGG GCCAAGTGCA CCCAAACTAC
    CTTTGGATGG TCTGTGGCAG CAGAGATGCC CCATCCGGCC GGGACTCCCG GCGGGGGCCA
    CCTCCAGAAG AACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001359416.1
    CDS1489..2406
    Translation

    Target ORF information:

    RefSeq Version NM_001372487.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCCGGTG ATGGTCTGAT GCATGAGGTG GTGAATGGGC TAATGGAACG GCCAGACTGG 
    GAGACTGCCA TCCAGAAACC CCTGTGTAGC CTCCCTGGAG GCTCCGGCAA TGCGCTGGCA
    GCTTCTGTGA ACCACTATGC TGGGTACGAG CAGGTGACTA ATGAAGACCT GCTCATCAAC
    TGCACACTGC TGTTGTGCCG CCGGCGCCTG TCACCCATGA ACCTGCTGTC CCTGCACACT
    GCTTCTGGGC TGCGGCTCTA TTCTGTGCTC AGTCTGTCCT GGGGCTTTGT TGCTGACGTG
    GACCTCGAGA GTGAGAAGTA CAGGCGCTTG GGGGAGATTC GTTTCACAGT GGGCACCTTC
    TTTCGCCTAG CAAGCCTGCG CATCTACCAA GGCCAACTGG CCTACCTTCC TGTAGGAACT
    GTGGCCTCTA AGAGACCCGC CTCTACACTG GTGCAGAAGG GCCCCGTCGA CACACACCTT
    GTTCCTCTGG AGGAGCCAGT GCCTTCTCAT TGGACTGTGG TACCAGAACA GGACTTCGTC
    CTGGTGCTGG TGCTGCTACA CACCCACCTG AGCTCCGAGC TGTTTGCAGC ACCCATGGGC
    CGCTGTGAGG CTGGTGTTAT GCATCTGTTC TACGTACGTG CGGGGGTGTC AAGGGCTGCG
    CTGCTGCGCC TCTTCCTGGC CATGCAGAAG GGCAAGCATA TGGAACTTGA CTGTCCATAC
    CTGGTTCATG TGCCCGTGGT TGCTTTCCGC CTGGAGCCCA GGAGCCAGAG GGGCGTGTTT
    TCTGTGGATG GAGAGCTGAT GGTATGTGAA GCTGTGCAGG GCCAAGTGCA CCCAAACTAC
    CTTTGGATGG TCTGTGGCAG CAGAGATGCC CCATCCGGCC GGGACTCCCG GCGGGGGCCA
    CCTCCAGAAG AACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version XM_006532673.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product sphingosine kinase 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532736.1
    Translation

    Target ORF information:

    RefSeq Version XM_006532673.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16499
    Clone ID Related Accession (Same CDS sequence) XM_006532673.2 , NM_001172472.1 , NM_025367.7 , NM_001172472.2 , NM_001372486.1 , NM_001372484.1 , NM_025367.6 , XM_006532673.3 , NM_001172473.1
    Accession Version XM_006532673.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532736.1
    CDS424..1572
    Translation

    Target ORF information:

    RefSeq Version XM_006532673.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532673.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGAACCAG TAGAATGCCC TCGAGGACTG CTCCCACGGC CATGCAGAGT GCTGGTGCTG 
    CTGAACCCCC AGGGTGGCAA GGGCAAGGCT CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC
    TTCCTGGAGG AGGCAGAGAT AACCTTTAAA CTGATACTCA CCGAACGGAA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT CACTGGGACG CCCTGGCAGT CATGTCCGGT
    GATGGTCTGA TGCATGAGGT GGTGAATGGG CTAATGGAAC GGCCAGACTG GGAGACTGCC
    ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG
    AACCACTATG CTGGGTACGA GCAGGTGACT AATGAAGACC TGCTCATCAA CTGCACACTG
    CTGTTGTGCC GCCGGCGCCT GTCACCCATG AACCTGCTGT CCCTGCACAC TGCTTCTGGG
    CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC TGGGGCTTTG TTGCTGACGT GGACCTCGAG
    AGTGAGAAGT ACAGGCGCTT GGGGGAGATT CGTTTCACAG TGGGCACCTT CTTTCGCCTA
    GCAAGCCTGC GCATCTACCA AGGCCAACTG GCCTACCTTC CTGTAGGAAC TGTGGCCTCT
    AAGAGACCCG CCTCTACACT GGTGCAGAAG GGCCCCGTCG ACACACACCT TGTTCCTCTG
    GAGGAGCCAG TGCCTTCTCA TTGGACTGTG GTACCAGAAC AGGACTTCGT CCTGGTGCTG
    GTGCTGCTAC ACACCCACCT GAGCTCCGAG CTGTTTGCAG CACCCATGGG CCGCTGTGAG
    GCTGGTGTTA TGCATCTGTT CTACGTACGT GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGAACTTG ACTGTCCATA CCTGGTTCAT
    GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT
    GGAGAGCTGA TGGTATGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTGTGGCA GCAGAGATGC CCCATCCGGC CGGGACTCCC GGCGGGGGCC ACCTCCAGAA
    GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45871
    Clone ID Related Accession (Same CDS sequence) XM_006532669.4 , XM_006532669.3
    Accession Version XM_006532669.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532669.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTCGGG TCCGAATCCG GTGTCCCCTG GAACGGAATG TTCGTGGGGT GGGAAGCTCA 
    TTCTGGAAAG CTAGATCCCC CACCCACGGG GCTCTGGTGA TTCAGTCGCG AGCCAGGGGC
    TCTGGTTGGG CACTTTGTCC CACGTGCATG GAAGCCCAGG CAGAGAACCC CACCACTCTT
    TCCTCCTGTC CCTCAGATCT GGCCGCCCCG GGGAATGACG AGGGCGCTCA CACAGCCCAG
    GGAGGTGGAG AGGGCGAGCC CCACGGCCAG TCGCCAGACA CCCTCCTGGG CAACACCGAT
    AAGAAGCTGA ACGCAGGAGC CGCCGTTACC TCTAGCAGCG CCGGGGCAGC ACCGGTGGCC
    CCTTGTCAGC GGGAGCCCCG GGACCTGGCT ATGGAACCAG AATGCCCTCG AGGACTGCTC
    CCACGGCCAT GCAGAGTGCT GGTGCTGCTG AACCCCCAGG GTGGCAAGGG CAAGGCTCTG
    CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC CTGGAGGAGG CAGAGATAAC CTTTAAACTG
    ATACTCACCG AACGGAAGAA CCATGCCAGG GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC
    TGGGACGCCC TGGCAGTCAT GTCCGGTGAT GGTCTGATGC ATGAGGTGGT GAATGGGCTA
    ATGGAACGGC CAGACTGGGA GACTGCCATC CAGAAACCCC TGTGTAGCCT CCCTGGAGGC
    TCCGGCAATG CGCTGGCAGC TTCTGTGAAC CACTATGCTG GGTACGAGCA GGTGACTAAT
    GAAGACCTGC TCATCAACTG CACACTGCTG TTGTGCCGCC GGCGCCTGTC ACCCATGAAC
    CTGCTGTCCC TGCACACTGC TTCTGGGCTG CGGCTCTATT CTGTGCTCAG TCTGTCCTGG
    GGCTTTGTTG CTGACGTGGA CCTCGAGAGT GAGAAGTACA GGCGCTTGGG GGAGATTCGT
    TTCACAGTGG GCACCTTCTT TCGCCTAGCA AGCCTGCGCA TCTACCAAGG CCAACTGGCC
    TACCTTCCTG TAGGAACTGT GGCCTCTAAG AGACCCGCCT CTACACTGGT GCAGAAGGGC
    CCCGTCGACA CACACCTTGT TCCTCTGGAG GAGCCAGTGC CTTCTCATTG GACTGTGGTA
    CCAGAACAGG ACTTCGTCCT GGTGCTGGTG CTGCTACACA CCCACCTGAG CTCCGAGCTG
    TTTGCAGCAC CCATGGGCCG CTGTGAGGCT GGTGTTATGC ATCTGTTCTA CGTACGTGCG
    GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC TTCCTGGCCA TGCAGAAGGG CAAGCATATG
    GAACTTGACT GTCCATACCT GGTTCATGTG CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG
    AGCCAGAGGG GCGTGTTTTC TGTGGATGGA GAGCTGATGG TATGTGAAGC TGTGCAGGGC
    CAAGTGCACC CAAACTACCT TTGGATGGTC TGTGGCAGCA GAGATGCCCC ATCCGGCCGG
    GACTCCCGGC GGGGGCCACC TCCAGAAGAA CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006532732.1
    CDS1792..3327
    Translation

    Target ORF information:

    RefSeq Version XM_006532669.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006532669.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTCGGG TCCGAATCCG GTGTCCCCTG GAACGGAATG TTCGTGGGGT GGGAAGCTCA 
    TTCTGGAAAG CTAGATCCCC CACCCACGGG GCTCTGGTGA TTCAGTCGCG AGCCAGGGGC
    TCTGGTTGGG CACTTTGTCC CACGTGCATG GAAGCCCAGG CAGAGAACCC CACCACTCTT
    TCCTCCTGTC CCTCAGATCT GGCCGCCCCG GGGAATGACG AGGGCGCTCA CACAGCCCAG
    GGAGGTGGAG AGGGCGAGCC CCACGGCCAG TCGCCAGACA CCCTCCTGGG CAACACCGAT
    AAGAAGCTGA ACGCAGGAGC CGCCGTTACC TCTAGCAGCG CCGGGGCAGC ACCGGTGGCC
    CCTTGTCAGC GGGAGCCCCG GGACCTGGCT ATGGAACCAG AATGCCCTCG AGGACTGCTC
    CCACGGCCAT GCAGAGTGCT GGTGCTGCTG AACCCCCAGG GTGGCAAGGG CAAGGCTCTG
    CAGCTCTTCC AGAGCCGTGT GCAGCCCTTC CTGGAGGAGG CAGAGATAAC CTTTAAACTG
    ATACTCACCG AACGGAAGAA CCATGCCAGG GAGCTGGTGT GTGCAGAGGA GTTGGGTCAC
    TGGGACGCCC TGGCAGTCAT GTCCGGTGAT GGTCTGATGC ATGAGGTGGT GAATGGGCTA
    ATGGAACGGC CAGACTGGGA GACTGCCATC CAGAAACCCC TGTGTAGCCT CCCTGGAGGC
    TCCGGCAATG CGCTGGCAGC TTCTGTGAAC CACTATGCTG GGTACGAGCA GGTGACTAAT
    GAAGACCTGC TCATCAACTG CACACTGCTG TTGTGCCGCC GGCGCCTGTC ACCCATGAAC
    CTGCTGTCCC TGCACACTGC TTCTGGGCTG CGGCTCTATT CTGTGCTCAG TCTGTCCTGG
    GGCTTTGTTG CTGACGTGGA CCTCGAGAGT GAGAAGTACA GGCGCTTGGG GGAGATTCGT
    TTCACAGTGG GCACCTTCTT TCGCCTAGCA AGCCTGCGCA TCTACCAAGG CCAACTGGCC
    TACCTTCCTG TAGGAACTGT GGCCTCTAAG AGACCCGCCT CTACACTGGT GCAGAAGGGC
    CCCGTCGACA CACACCTTGT TCCTCTGGAG GAGCCAGTGC CTTCTCATTG GACTGTGGTA
    CCAGAACAGG ACTTCGTCCT GGTGCTGGTG CTGCTACACA CCCACCTGAG CTCCGAGCTG
    TTTGCAGCAC CCATGGGCCG CTGTGAGGCT GGTGTTATGC ATCTGTTCTA CGTACGTGCG
    GGGGTGTCAA GGGCTGCGCT GCTGCGCCTC TTCCTGGCCA TGCAGAAGGG CAAGCATATG
    GAACTTGACT GTCCATACCT GGTTCATGTG CCCGTGGTTG CTTTCCGCCT GGAGCCCAGG
    AGCCAGAGGG GCGTGTTTTC TGTGGATGGA GAGCTGATGG TATGTGAAGC TGTGCAGGGC
    CAAGTGCACC CAAACTACCT TTGGATGGTC TGTGGCAGCA GAGATGCCCC ATCCGGCCGG
    GACTCCCGGC GGGGGCCACC TCCAGAAGAA CCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77820
    Clone ID Related Accession (Same CDS sequence) XM_017314398.1
    Accession Version XM_017314398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product sphingosine kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTCGGG TCCGAATCCG GTGTCCCCTG GAACGGAATG TTCGTGGGGT GGGAAGCTCA 
    TTCTGGAAAG CTAGATCCCC CACCCACGGG GCTCTGGTGA TTCAGTCGCG AGCCAGGGGC
    TCTGGTTGGG CACTTTGTCC CACGTGCATG GAAGCCCAGG CAGAGAACCC CACCACTCTT
    TCCTCCTGTC CCTCAGATCT GGCCGCCCCG GGGAATGACG AGGGCGCTCA CACAGCCCAG
    GGAGGTGGAG AGGGCGAGCC CCACGGCCAG TCGCCAGACA CCCTCCTGGG CAACACCGAT
    AAGAAGCTGA ACGCAGGAGC CGCCGTTACC TCTAGCAGCG CCGGGGCAGC ACCGGTGGCC
    CCTTGTCAGC GGGAGCCCCG GGACCTGGCT ATGGAACCAG TAGAATGCCC TCGAGGACTG
    CTCCCACGGC CATGCAGAGT GCTGGTGCTG CTGAACCCCC AGGGTGGCAA GGGCAAGGCT
    CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC TTCCTGGAGG AGGCAGAGAT AACCTTTAAA
    CTGATACTCA CCGAACGGAA GAACCATGCC AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT
    CACTGGGACG CCCTGGCAGT CATGTCCGGT GATGGTCTGA TGCATGAGGT GGTGAATGGG
    CTAATGGAAC GGCCAGACTG GGAGACTGCC ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA
    GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG AACCACTATG CTGGGTACGA GCAGGTGACT
    AATGAAGACC TGCTCATCAA CTGCACACTG CTGTTGTGCC GCCGGCGCCT GTCACCCATG
    AACCTGCTGT CCCTGCACAC TGCTTCTGGG CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC
    TGGGGCTTTG TTGCTGACGT GGACCTCGAG AGTGAGAAGT ACAGGCGCTT GGGGGAGATT
    CGTTTCACAG TGGGCACCTT CTTTCGCCTA GCAAGCCTGC GCATCTACCA AGGCCAACTG
    GCCTACCTTC CTGTAGGAAC TGTGGCCTCT AAGAGACCCG CCTCTACACT GGTGCAGAAG
    GGCCCCGTCG ACACACACCT TGTTCCTCTG GAGGAGCCAG TGCCTTCTCA TTGGACTGTG
    GTACCAGAAC AGGACTTCGT CCTGGTGCTG GTGCTGCTAC ACACCCACCT GAGCTCCGAG
    CTGTTTGCAG CACCCATGGG CCGCTGTGAG GCTGGTGTTA TGCATCTGTT CTACGTACGT
    GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC CTCTTCCTGG CCATGCAGAA GGGCAAGCAT
    ATGGAACTTG ACTGTCCATA CCTGGTTCAT GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC
    AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT GGAGAGCTGA TGGTATGTGA AGCTGTGCAG
    GGCCAAGTGC ACCCAAACTA CCTTTGGATG GTCTGTGGCA GCAGAGATGC CCCATCCGGC
    CGGGACTCCC GGCGGGGGCC ACCTCCAGAA GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169887.1
    CDS1668..3206
    Translation

    Target ORF information:

    RefSeq Version XM_017314398.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017314398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTCGGG TCCGAATCCG GTGTCCCCTG GAACGGAATG TTCGTGGGGT GGGAAGCTCA 
    TTCTGGAAAG CTAGATCCCC CACCCACGGG GCTCTGGTGA TTCAGTCGCG AGCCAGGGGC
    TCTGGTTGGG CACTTTGTCC CACGTGCATG GAAGCCCAGG CAGAGAACCC CACCACTCTT
    TCCTCCTGTC CCTCAGATCT GGCCGCCCCG GGGAATGACG AGGGCGCTCA CACAGCCCAG
    GGAGGTGGAG AGGGCGAGCC CCACGGCCAG TCGCCAGACA CCCTCCTGGG CAACACCGAT
    AAGAAGCTGA ACGCAGGAGC CGCCGTTACC TCTAGCAGCG CCGGGGCAGC ACCGGTGGCC
    CCTTGTCAGC GGGAGCCCCG GGACCTGGCT ATGGAACCAG TAGAATGCCC TCGAGGACTG
    CTCCCACGGC CATGCAGAGT GCTGGTGCTG CTGAACCCCC AGGGTGGCAA GGGCAAGGCT
    CTGCAGCTCT TCCAGAGCCG TGTGCAGCCC TTCCTGGAGG AGGCAGAGAT AACCTTTAAA
    CTGATACTCA CCGAACGGAA GAACCATGCC AGGGAGCTGG TGTGTGCAGA GGAGTTGGGT
    CACTGGGACG CCCTGGCAGT CATGTCCGGT GATGGTCTGA TGCATGAGGT GGTGAATGGG
    CTAATGGAAC GGCCAGACTG GGAGACTGCC ATCCAGAAAC CCCTGTGTAG CCTCCCTGGA
    GGCTCCGGCA ATGCGCTGGC AGCTTCTGTG AACCACTATG CTGGGTACGA GCAGGTGACT
    AATGAAGACC TGCTCATCAA CTGCACACTG CTGTTGTGCC GCCGGCGCCT GTCACCCATG
    AACCTGCTGT CCCTGCACAC TGCTTCTGGG CTGCGGCTCT ATTCTGTGCT CAGTCTGTCC
    TGGGGCTTTG TTGCTGACGT GGACCTCGAG AGTGAGAAGT ACAGGCGCTT GGGGGAGATT
    CGTTTCACAG TGGGCACCTT CTTTCGCCTA GCAAGCCTGC GCATCTACCA AGGCCAACTG
    GCCTACCTTC CTGTAGGAAC TGTGGCCTCT AAGAGACCCG CCTCTACACT GGTGCAGAAG
    GGCCCCGTCG ACACACACCT TGTTCCTCTG GAGGAGCCAG TGCCTTCTCA TTGGACTGTG
    GTACCAGAAC AGGACTTCGT CCTGGTGCTG GTGCTGCTAC ACACCCACCT GAGCTCCGAG
    CTGTTTGCAG CACCCATGGG CCGCTGTGAG GCTGGTGTTA TGCATCTGTT CTACGTACGT
    GCGGGGGTGT CAAGGGCTGC GCTGCTGCGC CTCTTCCTGG CCATGCAGAA GGGCAAGCAT
    ATGGAACTTG ACTGTCCATA CCTGGTTCAT GTGCCCGTGG TTGCTTTCCG CCTGGAGCCC
    AGGAGCCAGA GGGGCGTGTT TTCTGTGGAT GGAGAGCTGA TGGTATGTGA AGCTGTGCAG
    GGCCAAGTGC ACCCAAACTA CCTTTGGATG GTCTGTGGCA GCAGAGATGC CCCATCCGGC
    CGGGACTCCC GGCGGGGGCC ACCTCCAGAA GAACCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.