Six4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Six4
Entrez Gene ID	20474
Full Name	sine oculis-related homeobox 4
Synonyms	AI047561,AREC3,TrexBF
General protein information	Preferred Names sine oculis-related homeobox 4 Names homeobox protein SIX4 sine oculis homeobox homolog 4 sine oculis-related homeobox 4 homolog skeletal muscle-specific ARE-binding protein AREC3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362272.1	NP_001349201.1	homeobox protein SIX4 isoform 2
NM_011382.3	NP_035512.1	homeobox protein SIX4 isoform 1
XM_006515632.4	XP_006515695.1	homeobox protein SIX4 isoform X1
XM_006515632.3	XP_006515695.1	homeobox protein SIX4 isoform X1
NM_011382.2	NP_035512.1	homeobox protein SIX4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	six4a	NP_571793.2
Xenopus tropicalis (tropical clawed frog)	six4	XP_002933781.2
Homo sapiens (human)	SIX4	NP_059116.3
Danio rerio (zebrafish)	six4b	NP_571792.1
Mus musculus (house mouse)	Six4	NP_035512.1
	SIX4	XP_001096803.2
Pan troglodytes (chimpanzee)	SIX4	XP_003314345.1
Canis lupus familiaris (dog)	SIX4	XP_547842.2
Rattus norvegicus (Norway rat)	Six4	NP_001100209.1
Gallus gallus (chicken)	SIX4	XP_003641490.1

Gene Ontology
Bibliography

Related articles in PubMed

Specification of jaw identity by the Hand2 transcription factor.
Funato N, Kokubo H, Nakamura M, Yanagisawa H, Saga Y
Scientific reports628405(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

significant co-localization of binding sites for MyoD and Six proteins on over a thousand mouse genomic DNA regions, were found.
Title: MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis.

The results indicated that low Six4 and Six5 gene dosage improved dystrophic phenotypes of Dmd(mdx) /Y mice by enhancing muscle regeneration, and suggested that SIX4 and SIX5 are potentially useful de novo targets in therapeutic applications against muscle disorders, including DMD.
Title: Low Six4 and Six5 gene dosage improves dystrophic phenotype and prolongs life span of mdx mice.

results reveal a role for Six4 during adult muscle regeneration and suggest a widespread mechanism of cooperation between Six4 and MyoD.
Title: Genome-wide association between Six4, MyoD, and the histone demethylase Utx during myogenesis.

data support a model where Eya-Six may form a complex to regulate nephron progenitor cell development before metanephric specification and are critical mesenchymal factors for inducing nephric duct development.
Title: Eya-six are necessary for survival of nephrogenic cord progenitors and inducing nephric duct development before ureteric bud formation.

the transcription factors Six1 and Six4 are required for male gonadal differentiation.
Title: Homeoproteins Six1 and Six4 regulate male sex determination and mouse gonadal development.

The following Six4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Six4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20018	NM_011382.3 Latest version!	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu84251	NM_001362272.1 Latest version!	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu47690	XM_006515632.4 Latest version!	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu47690	XM_006515632.3	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu20018	NM_011382.2	Mus musculus sine oculis-related homeobox 4 (Six4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Six4 ( XM_006515632.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515695.1 Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47690
Clone ID Related Accession (Same CDS sequence)	XM_006515632.4 , XM_006515632.3
Accession Version	XM_006515632.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	homeobox protein SIX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCAA GTGCGGCGGA CATCAAGCAG GAGAATGGGA TGGAAAGCGC CTCGGAAGGA 
CAGGAGGCGC ACCGAGAAGT GGCGGGGGGC GCGGCGGCTG GGCTGAGCCC CCCGGCTCCA 
GCTCCTTTCC CCCTGGAGCC GGGGGACGCC GCGGCTGCCT CCAGGGTAAG CCGGGAGGAA 
GGGGCAGCGG CGGCGGGAGC GGCAGATCAG GTACAACTCC ACTCGGAACT TCTGGGCAGG 
CACCAGCACG CAGCCGCCGC GCAGCCCCCA CTGGCCTTCT CGCCGGACCA TGTCGCCTGC 
GTGTGCGAGG CGTTGCAGCA GGGGGGCAAC CTGGACCGCC TGGCCCGGTT TCTTTGGTCC 
CTGCCCCAGA GCGATCTGCT ACGTGGCAAC GAGAGCCTTC TGAAGGCGCG AGCGCTCGTG 
GCCTTCCACC AGGGCATCTA CCCTGAGTTG TACAGCATCC TCGAGAGCCA CAGCTTCGAG 
TCGGCCAACC ATCCGCTGCT GCAGCAGCTC TGGTACAAGG CGCGCTACAC CGAGGCCGAG 
CGAGCGCGCG GCCGGCCGCT GGGCGCCGTG GACAAGTACC GGCTGCGCAG GAAATTCCCC 
TTGCCCCGCA CCATCTGGGA CGGCGAGGAG ACGGTGTATT GTTTCAAGGA GAAGTCGCGC 
AACGCGCTCA AGGAGCTCTA CAAGCAGAAT CGCTACCCCT CGCCGGCTGA GAAGCGGCAC 
CTGGCCAAGA TCACCGGCCT CTCCCTCACC CAGGTCAGCA ACTGGTTCAA GAACCGGCGG 
CAGCGTGACC GAAACCCCTC CGAGACCCAG TCCAAAAGCG AATCGGATGG CAACCCCAGT 
ACCGAGGATG AATCCAGCAA GGGACATGAG GATTTGTCTC CTCATCCACT TTCAGGCGCA 
TCTGATGGCG TCACCAACCT CAGCCTCTCT AGCCACGTGG AGCCAGTATA TATGCAACAA 
ATTGGAAATG CTAAGATATC ACTAAGCTCG TCTGGAGTTT TGTTGAATGG AAGCTTAGTA 
CCTGCAAGTA CTTCACCTGT CTTCCTTAAT GGCAATTCTT TTATTCAGGG ACACAATGGA 
GTTATCCTTA ATGGACTGAA TGTGGGAAAT ACACAGACAG TGTCACTGAA CCCACCCAAA 
ATGTCTTCAA ACATTGTGGG CAATGGCATA GCCATGACAG ACATCCTGGG ATCTACCTCC 
CAGGATGTGA AAGAATTCAA AGTCCTCCAG AGTTCTGCTG TGAACTCAGC AGCCACCACC 
TCCTACAGCC CTAGTGCCCC TGTGTCATTC CCAGGGCTGA TACCCTGCAC TGAAGTGAAA 
AGAGAAGGCA TTCAGACAGT GGCCTCCCAG GACGGTGGCT CTGTGGTGAC TTTTACTACA 
CCCGTGCAAA TTAACCAGTA TGGCATTGTC CAGATCCCTA ATTCTGGAGC CAACGGCCAG 
TTCCTTAATG GGAGCATTGG ATTCTCTCCA CTGCAGCTGC CTCCTGTCTC AGTAGCAGCT 
TCACAAGGTA ATCTTTCAGT TACCCCAAGC ACTTCGGACG GAAGCACATT TACAAGTGAG 
CCAGCCACAG TCCAGCACGG TAAACTTTTC CTGAGCCCTC TTACCCCCAG TGCAGTGGTA 
TACACCGTTC CTAATTCAGG CCAGACTGTA GGAGCTGTGA AACAGGAAGG CTTAGAAAGA 
GGCCTGGTCT TTTCTCAGTT GATGCCTGTC AATCACAGTG CACAAGTAAA TGCAAGCCTG 
TCTTCGGAAA ATCTCTCTGG GAGTGGCCTC CATCCTCTGA CCTCGTCGTT GGTTAATGTG 
TCCGCCGCCC ACGGTTTTTC CCTGACCCCC CCTACACTAC TTAATCCCAC TGAGCTCAAC 
CCTGACCTTG CCGAGAGCCA GCCCGTGTCT GCACCTGTGG CAAGCAAATG TACTGTGTCA 
TCTGTCAGCA ACACTAACTA TGCAACCCTT CAGAACTGTT CCCTTATTCC GGGGCAAGAC 
CTATTGTCCG GCCCGATGAC CCAGGCTGCC CTGGGTGAGA TCGTACCCAC AGCTGAAGAG 
CAGGTGAGCC ACGCCTCAAC AGCAGTCCAC CAGGATTTTG TCAGAGAACA GCGCTTGGTT 
CTGCAGTCAG TCCCTAACAT AAAAGAGAAT TTCTTACAAA ATTCTGAGAA CAAAGCAACA 
AACAACTTAA TGATGTTAGA CTCGAAATCC AAGTACGTCC TGGACGGCAT GGTTGAAGCC 
GGCTGTGAAG ACCTGGGAAC GGACAAAAAA GAGCTGGCCA AGCTCCAAAC CGTCCAGTTG 
GATGAAGACA TGCAAGACTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515695.1
CDS	1405..3708
Translation	MIASAADIKQENGMESASEGQEAHREVAGGAAAGLSPPAPAPFPLEPGDAAAASRVSREEGAAAAGAADQVQLHSELLGRHQHAAAAQPPLAFSPDHVACVCEALQQGGNLDRLARFLWSLPQSDLLRGNESLLKARALVAFHQGIYPELYSILESHSFESANHPLLQQLWYKARYTEAERARGRPLGAVDKYRLRRKFPLPRTIWDGEETVYCFKEKSRNALKELYKQNRYPSPAEKRHLAKITGLSLTQVSNWFKNRRQRDRNPSETQSKSESDGNPSTEDESSKGHEDLSPHPLSGASDGVTNLSLSSHVEPVYMQQIGNAKISLSSSGVLLNGSLVPASTSPVFLNGNSFIQGHNGVILNGLNVGNTQTVSLNPPKMSSNIVGNGIAMTDILGSTSQDVKEFKVLQSSAVNSAATTSYSPSAPVSFPGLIPCTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANGQFLNGSIGFSPLQLPPVSVAASQGNLSVTPSTSDGSTFTSEPATVQHGKLFLSPLTPSAVVYTVPNSGQTVGAVKQEGLERGLVFSQLMPVNHSAQVNASLSSENLSGSGLHPLTSSLVNVSAAHGFSLTPPTLLNPTELNPDLAESQPVSAPVASKCTVSSVSNTNYATLQNCSLIPGQDLLSGPMTQAALGEIVPTAEEQVSHASTAVHQDFVREQRLVLQSVPNIKENFLQNSENKATNNLMMLDSKSKYVLDGMVEAGCEDLGTDKKELAKLQTVQLDEDMQDL

Target ORF information:

RefSeq Version	XM_006515632.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515632.3

ORF Insert Sequence:

ATGATTGCAA GTGCGGCGGA CATCAAGCAG GAGAATGGGA TGGAAAGCGC CTCGGAAGGA 
CAGGAGGCGC ACCGAGAAGT GGCGGGGGGC GCGGCGGCTG GGCTGAGCCC CCCGGCTCCA 
GCTCCTTTCC CCCTGGAGCC GGGGGACGCC GCGGCTGCCT CCAGGGTAAG CCGGGAGGAA 
GGGGCAGCGG CGGCGGGAGC GGCAGATCAG GTACAACTCC ACTCGGAACT TCTGGGCAGG 
CACCAGCACG CAGCCGCCGC GCAGCCCCCA CTGGCCTTCT CGCCGGACCA TGTCGCCTGC 
GTGTGCGAGG CGTTGCAGCA GGGGGGCAAC CTGGACCGCC TGGCCCGGTT TCTTTGGTCC 
CTGCCCCAGA GCGATCTGCT ACGTGGCAAC GAGAGCCTTC TGAAGGCGCG AGCGCTCGTG 
GCCTTCCACC AGGGCATCTA CCCTGAGTTG TACAGCATCC TCGAGAGCCA CAGCTTCGAG 
TCGGCCAACC ATCCGCTGCT GCAGCAGCTC TGGTACAAGG CGCGCTACAC CGAGGCCGAG 
CGAGCGCGCG GCCGGCCGCT GGGCGCCGTG GACAAGTACC GGCTGCGCAG GAAATTCCCC 
TTGCCCCGCA CCATCTGGGA CGGCGAGGAG ACGGTGTATT GTTTCAAGGA GAAGTCGCGC 
AACGCGCTCA AGGAGCTCTA CAAGCAGAAT CGCTACCCCT CGCCGGCTGA GAAGCGGCAC 
CTGGCCAAGA TCACCGGCCT CTCCCTCACC CAGGTCAGCA ACTGGTTCAA GAACCGGCGG 
CAGCGTGACC GAAACCCCTC CGAGACCCAG TCCAAAAGCG AATCGGATGG CAACCCCAGT 
ACCGAGGATG AATCCAGCAA GGGACATGAG GATTTGTCTC CTCATCCACT TTCAGGCGCA 
TCTGATGGCG TCACCAACCT CAGCCTCTCT AGCCACGTGG AGCCAGTATA TATGCAACAA 
ATTGGAAATG CTAAGATATC ACTAAGCTCG TCTGGAGTTT TGTTGAATGG AAGCTTAGTA 
CCTGCAAGTA CTTCACCTGT CTTCCTTAAT GGCAATTCTT TTATTCAGGG ACACAATGGA 
GTTATCCTTA ATGGACTGAA TGTGGGAAAT ACACAGACAG TGTCACTGAA CCCACCCAAA 
ATGTCTTCAA ACATTGTGGG CAATGGCATA GCCATGACAG ACATCCTGGG ATCTACCTCC 
CAGGATGTGA AAGAATTCAA AGTCCTCCAG AGTTCTGCTG TGAACTCAGC AGCCACCACC 
TCCTACAGCC CTAGTGCCCC TGTGTCATTC CCAGGGCTGA TACCCTGCAC TGAAGTGAAA 
AGAGAAGGCA TTCAGACAGT GGCCTCCCAG GACGGTGGCT CTGTGGTGAC TTTTACTACA 
CCCGTGCAAA TTAACCAGTA TGGCATTGTC CAGATCCCTA ATTCTGGAGC CAACGGCCAG 
TTCCTTAATG GGAGCATTGG ATTCTCTCCA CTGCAGCTGC CTCCTGTCTC AGTAGCAGCT 
TCACAAGGTA ATCTTTCAGT TACCCCAAGC ACTTCGGACG GAAGCACATT TACAAGTGAG 
CCAGCCACAG TCCAGCACGG TAAACTTTTC CTGAGCCCTC TTACCCCCAG TGCAGTGGTA 
TACACCGTTC CTAATTCAGG CCAGACTGTA GGAGCTGTGA AACAGGAAGG CTTAGAAAGA 
GGCCTGGTCT TTTCTCAGTT GATGCCTGTC AATCACAGTG CACAAGTAAA TGCAAGCCTG 
TCTTCGGAAA ATCTCTCTGG GAGTGGCCTC CATCCTCTGA CCTCGTCGTT GGTTAATGTG 
TCCGCCGCCC ACGGTTTTTC CCTGACCCCC CCTACACTAC TTAATCCCAC TGAGCTCAAC 
CCTGACCTTG CCGAGAGCCA GCCCGTGTCT GCACCTGTGG CAAGCAAATG TACTGTGTCA 
TCTGTCAGCA ACACTAACTA TGCAACCCTT CAGAACTGTT CCCTTATTCC GGGGCAAGAC 
CTATTGTCCG GCCCGATGAC CCAGGCTGCC CTGGGTGAGA TCGTACCCAC AGCTGAAGAG 
CAGGTGAGCC ACGCCTCAAC AGCAGTCCAC CAGGATTTTG TCAGAGAACA GCGCTTGGTT 
CTGCAGTCAG TCCCTAACAT AAAAGAGAAT TTCTTACAAA ATTCTGAGAA CAAAGCAACA 
AACAACTTAA TGATGTTAGA CTCGAAATCC AAGTACGTCC TGGACGGCAT GGTTGAAGCC 
GGCTGTGAAG ACCTGGGAAC GGACAAAAAA GAGCTGGCCA AGCTCCAAAC CGTCCAGTTG 
GATGAAGACA TGCAAGACTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Six4 ( NM_011382.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035512.1 Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20018
Clone ID Related Accession (Same CDS sequence)	NM_011382.3 , NM_011382.2
Accession Version	NM_011382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	homeobox protein SIX4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159274.2. On Apr 11, 2018 this sequence version replaced NM_011382.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D50416.1, BC137931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTCTT CCTCCCCCAC CGGGCAGATT GCAAGTGCGG CGGACATCAA GCAGGAGAAT 
GGGATGGAAA GCGCCTCGGA AGGACAGGAG GCGCACCGAG AAGTGGCGGG GGGCGCGGCG 
GCTGGGCTGA GCCCCCCGGC TCCAGCTCCT TTCCCCCTGG AGCCGGGGGA CGCCGCGGCT 
GCCTCCAGGG TAAGCCGGGA GGAAGGGGCA GCGGCGGCGG GAGCGGCAGA TCAGGTACAA 
CTCCACTCGG AACTTCTGGG CAGGCACCAG CACGCAGCCG CCGCGCAGCC CCCACTGGCC 
TTCTCGCCGG ACCATGTCGC CTGCGTGTGC GAGGCGTTGC AGCAGGGGGG CAACCTGGAC 
CGCCTGGCCC GGTTTCTTTG GTCCCTGCCC CAGAGCGATC TGCTACGTGG CAACGAGAGC 
CTTCTGAAGG CGCGAGCGCT CGTGGCCTTC CACCAGGGCA TCTACCCTGA GTTGTACAGC 
ATCCTCGAGA GCCACAGCTT CGAGTCGGCC AACCATCCGC TGCTGCAGCA GCTCTGGTAC 
AAGGCGCGCT ACACCGAGGC CGAGCGAGCG CGCGGCCGGC CGCTGGGCGC CGTGGACAAG 
TACCGGCTGC GCAGGAAATT CCCCTTGCCC CGCACCATCT GGGACGGCGA GGAGACGGTG 
TATTGTTTCA AGGAGAAGTC GCGCAACGCG CTCAAGGAGC TCTACAAGCA GAATCGCTAC 
CCCTCGCCGG CTGAGAAGCG GCACCTGGCC AAGATCACCG GCCTCTCCCT CACCCAGGTC 
AGCAACTGGT TCAAGAACCG GCGGCAGCGT GACCGAAACC CCTCCGAGAC CCAGTCCAAA 
AGCGAATCGG ATGGCAACCC CAGTACCGAG GATGAATCCA GCAAGGGACA TGAGGATTTG 
TCTCCTCATC CACTTTCAGG CGCATCTGAT GGCGTCACCA ACCTCAGCCT CTCTAGCCAC 
GTGGAGCCAG TATATATGCA ACAAATTGGA AATGCTAAGA TATCACTAAG CTCGTCTGGA 
GTTTTGTTGA ATGGAAGCTT AGTACCTGCA AGTACTTCAC CTGTCTTCCT TAATGGCAAT 
TCTTTTATTC AGGGACACAA TGGAGTTATC CTTAATGGAC TGAATGTGGG AAATACACAG 
ACAGTGTCAC TGAACCCACC CAAAATGTCT TCAAACATTG TGGGCAATGG CATAGCCATG 
ACAGACATCC TGGGATCTAC CTCCCAGGAT GTGAAAGAAT TCAAAGTCCT CCAGAGTTCT 
GCTGTGAACT CAGCAGCCAC CACCTCCTAC AGCCCTAGTG CCCCTGTGTC ATTCCCAGGG 
CTGATACCCT GCACTGAAGT GAAAAGAGAA GGCATTCAGA CAGTGGCCTC CCAGGACGGT 
GGCTCTGTGG TGACTTTTAC TACACCCGTG CAAATTAACC AGTATGGCAT TGTCCAGATC 
CCTAATTCTG GAGCCAACGG CCAGTTCCTT AATGGGAGCA TTGGATTCTC TCCACTGCAG 
CTGCCTCCTG TCTCAGTAGC AGCTTCACAA GGTAATCTTT CAGTTACCCC AAGCACTTCG 
GACGGAAGCA CATTTACAAG TGAGCCAGCC ACAGTCCAGC ACGGTAAACT TTTCCTGAGC 
CCTCTTACCC CCAGTGCAGT GGTATACACC GTTCCTAATT CAGGCCAGAC TGTAGGAGCT 
GTGAAACAGG AAGGCTTAGA AAGAGGCCTG GTCTTTTCTC AGTTGATGCC TGTCAATCAC 
AGTGCACAAG TAAATGCAAG CCTGTCTTCG GAAAATCTCT CTGGGAGTGG CCTCCATCCT 
CTGACCTCGT CGTTGGTTAA TGTGTCCGCC GCCCACGGTT TTTCCCTGAC CCCCCCTACA 
CTACTTAATC CCACTGAGCT CAACCCTGAC CTTGCCGAGA GCCAGCCCGT GTCTGCACCT 
GTGGCAAGCA AATGTACTGT GTCATCTGTC AGCAACACTA ACTATGCAAC CCTTCAGAAC 
TGTTCCCTTA TTCCGGGGCA AGACCTATTG TCCGGCCCGA TGACCCAGGC TGCCCTGGGT 
GAGATCGTAC CCACAGCTGA AGAGCAGGTG AGCCACGCCT CAACAGCAGT CCACCAGGAT 
TTTGTCAGAG AACAGCGCTT GGTTCTGCAG TCAGTCCCTA ACATAAAAGA GAATTTCTTA 
CAAAATTCTG AGAACAAAGC AACAAACAAC TTAATGATGT TAGACTCGAA ATCCAAGTAC 
GTCCTGGACG GCATGGTTGA AGCCGGCTGT GAAGACCTGG GAACGGACAA AAAAGAGCTG 
GCCAAGCTCC AAACCGTCCA GTTGGATGAA GACATGCAAG ACTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035512.1
CDS	62..2389
Translation	MSSSSPTGQIASAADIKQENGMESASEGQEAHREVAGGAAAGLSPPAPAPFPLEPGDAAAASRVSREEGAAAAGAADQVQLHSELLGRHQHAAAAQPPLAFSPDHVACVCEALQQGGNLDRLARFLWSLPQSDLLRGNESLLKARALVAFHQGIYPELYSILESHSFESANHPLLQQLWYKARYTEAERARGRPLGAVDKYRLRRKFPLPRTIWDGEETVYCFKEKSRNALKELYKQNRYPSPAEKRHLAKITGLSLTQVSNWFKNRRQRDRNPSETQSKSESDGNPSTEDESSKGHEDLSPHPLSGASDGVTNLSLSSHVEPVYMQQIGNAKISLSSSGVLLNGSLVPASTSPVFLNGNSFIQGHNGVILNGLNVGNTQTVSLNPPKMSSNIVGNGIAMTDILGSTSQDVKEFKVLQSSAVNSAATTSYSPSAPVSFPGLIPCTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANGQFLNGSIGFSPLQLPPVSVAASQGNLSVTPSTSDGSTFTSEPATVQHGKLFLSPLTPSAVVYTVPNSGQTVGAVKQEGLERGLVFSQLMPVNHSAQVNASLSSENLSGSGLHPLTSSLVNVSAAHGFSLTPPTLLNPTELNPDLAESQPVSAPVASKCTVSSVSNTNYATLQNCSLIPGQDLLSGPMTQAALGEIVPTAEEQVSHASTAVHQDFVREQRLVLQSVPNIKENFLQNSENKATNNLMMLDSKSKYVLDGMVEAGCEDLGTDKKELAKLQTVQLDEDMQDL

Target ORF information:

RefSeq Version	NM_011382.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011382.3

ORF Insert Sequence:

ATGTCCTCTT CCTCCCCCAC CGGGCAGATT GCAAGTGCGG CGGACATCAA GCAGGAGAAT 
GGGATGGAAA GCGCCTCGGA AGGACAGGAG GCGCACCGAG AAGTGGCGGG GGGCGCGGCG 
GCTGGGCTGA GCCCCCCGGC TCCAGCTCCT TTCCCCCTGG AGCCGGGGGA CGCCGCGGCT 
GCCTCCAGGG TAAGCCGGGA GGAAGGGGCA GCGGCGGCGG GAGCGGCAGA TCAGGTACAA 
CTCCACTCGG AACTTCTGGG CAGGCACCAG CACGCAGCCG CCGCGCAGCC CCCACTGGCC 
TTCTCGCCGG ACCATGTCGC CTGCGTGTGC GAGGCGTTGC AGCAGGGGGG CAACCTGGAC 
CGCCTGGCCC GGTTTCTTTG GTCCCTGCCC CAGAGCGATC TGCTACGTGG CAACGAGAGC 
CTTCTGAAGG CGCGAGCGCT CGTGGCCTTC CACCAGGGCA TCTACCCTGA GTTGTACAGC 
ATCCTCGAGA GCCACAGCTT CGAGTCGGCC AACCATCCGC TGCTGCAGCA GCTCTGGTAC 
AAGGCGCGCT ACACCGAGGC CGAGCGAGCG CGCGGCCGGC CGCTGGGCGC CGTGGACAAG 
TACCGGCTGC GCAGGAAATT CCCCTTGCCC CGCACCATCT GGGACGGCGA GGAGACGGTG 
TATTGTTTCA AGGAGAAGTC GCGCAACGCG CTCAAGGAGC TCTACAAGCA GAATCGCTAC 
CCCTCGCCGG CTGAGAAGCG GCACCTGGCC AAGATCACCG GCCTCTCCCT CACCCAGGTC 
AGCAACTGGT TCAAGAACCG GCGGCAGCGT GACCGAAACC CCTCCGAGAC CCAGTCCAAA 
AGCGAATCGG ATGGCAACCC CAGTACCGAG GATGAATCCA GCAAGGGACA TGAGGATTTG 
TCTCCTCATC CACTTTCAGG CGCATCTGAT GGCGTCACCA ACCTCAGCCT CTCTAGCCAC 
GTGGAGCCAG TATATATGCA ACAAATTGGA AATGCTAAGA TATCACTAAG CTCGTCTGGA 
GTTTTGTTGA ATGGAAGCTT AGTACCTGCA AGTACTTCAC CTGTCTTCCT TAATGGCAAT 
TCTTTTATTC AGGGACACAA TGGAGTTATC CTTAATGGAC TGAATGTGGG AAATACACAG 
ACAGTGTCAC TGAACCCACC CAAAATGTCT TCAAACATTG TGGGCAATGG CATAGCCATG 
ACAGACATCC TGGGATCTAC CTCCCAGGAT GTGAAAGAAT TCAAAGTCCT CCAGAGTTCT 
GCTGTGAACT CAGCAGCCAC CACCTCCTAC AGCCCTAGTG CCCCTGTGTC ATTCCCAGGG 
CTGATACCCT GCACTGAAGT GAAAAGAGAA GGCATTCAGA CAGTGGCCTC CCAGGACGGT 
GGCTCTGTGG TGACTTTTAC TACACCCGTG CAAATTAACC AGTATGGCAT TGTCCAGATC 
CCTAATTCTG GAGCCAACGG CCAGTTCCTT AATGGGAGCA TTGGATTCTC TCCACTGCAG 
CTGCCTCCTG TCTCAGTAGC AGCTTCACAA GGTAATCTTT CAGTTACCCC AAGCACTTCG 
GACGGAAGCA CATTTACAAG TGAGCCAGCC ACAGTCCAGC ACGGTAAACT TTTCCTGAGC 
CCTCTTACCC CCAGTGCAGT GGTATACACC GTTCCTAATT CAGGCCAGAC TGTAGGAGCT 
GTGAAACAGG AAGGCTTAGA AAGAGGCCTG GTCTTTTCTC AGTTGATGCC TGTCAATCAC 
AGTGCACAAG TAAATGCAAG CCTGTCTTCG GAAAATCTCT CTGGGAGTGG CCTCCATCCT 
CTGACCTCGT CGTTGGTTAA TGTGTCCGCC GCCCACGGTT TTTCCCTGAC CCCCCCTACA 
CTACTTAATC CCACTGAGCT CAACCCTGAC CTTGCCGAGA GCCAGCCCGT GTCTGCACCT 
GTGGCAAGCA AATGTACTGT GTCATCTGTC AGCAACACTA ACTATGCAAC CCTTCAGAAC 
TGTTCCCTTA TTCCGGGGCA AGACCTATTG TCCGGCCCGA TGACCCAGGC TGCCCTGGGT 
GAGATCGTAC CCACAGCTGA AGAGCAGGTG AGCCACGCCT CAACAGCAGT CCACCAGGAT 
TTTGTCAGAG AACAGCGCTT GGTTCTGCAG TCAGTCCCTA ACATAAAAGA GAATTTCTTA 
CAAAATTCTG AGAACAAAGC AACAAACAAC TTAATGATGT TAGACTCGAA ATCCAAGTAC 
GTCCTGGACG GCATGGTTGA AGCCGGCTGT GAAGACCTGG GAACGGACAA AAAAGAGCTG 
GCCAAGCTCC AAACCGTCCA GTTGGATGAA GACATGCAAG ACTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Six4 ( NM_011382.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035512.1 Mus musculus sine oculis-related homeobox 4 (Six4), mRNA.

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CloneID	OMu20018
Clone ID Related Accession (Same CDS sequence)	NM_011382.3 , NM_011382.2
Accession Version	NM_011382.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	homeobox protein SIX4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC159274.2. On Nov 17, 2006 this sequence version replaced NM_011382.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D50416.1, BC137931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTCTT CCTCCCCCAC CGGGCAGATT GCAAGTGCGG CGGACATCAA GCAGGAGAAT 
GGGATGGAAA GCGCCTCGGA AGGACAGGAG GCGCACCGAG AAGTGGCGGG GGGCGCGGCG 
GCTGGGCTGA GCCCCCCGGC TCCAGCTCCT TTCCCCCTGG AGCCGGGGGA CGCCGCGGCT 
GCCTCCAGGG TAAGCCGGGA GGAAGGGGCA GCGGCGGCGG GAGCGGCAGA TCAGGTACAA 
CTCCACTCGG AACTTCTGGG CAGGCACCAG CACGCAGCCG CCGCGCAGCC CCCACTGGCC 
TTCTCGCCGG ACCATGTCGC CTGCGTGTGC GAGGCGTTGC AGCAGGGGGG CAACCTGGAC 
CGCCTGGCCC GGTTTCTTTG GTCCCTGCCC CAGAGCGATC TGCTACGTGG CAACGAGAGC 
CTTCTGAAGG CGCGAGCGCT CGTGGCCTTC CACCAGGGCA TCTACCCTGA GTTGTACAGC 
ATCCTCGAGA GCCACAGCTT CGAGTCGGCC AACCATCCGC TGCTGCAGCA GCTCTGGTAC 
AAGGCGCGCT ACACCGAGGC CGAGCGAGCG CGCGGCCGGC CGCTGGGCGC CGTGGACAAG 
TACCGGCTGC GCAGGAAATT CCCCTTGCCC CGCACCATCT GGGACGGCGA GGAGACGGTG 
TATTGTTTCA AGGAGAAGTC GCGCAACGCG CTCAAGGAGC TCTACAAGCA GAATCGCTAC 
CCCTCGCCGG CTGAGAAGCG GCACCTGGCC AAGATCACCG GCCTCTCCCT CACCCAGGTC 
AGCAACTGGT TCAAGAACCG GCGGCAGCGT GACCGAAACC CCTCCGAGAC CCAGTCCAAA 
AGCGAATCGG ATGGCAACCC CAGTACCGAG GATGAATCCA GCAAGGGACA TGAGGATTTG 
TCTCCTCATC CACTTTCAGG CGCATCTGAT GGCGTCACCA ACCTCAGCCT CTCTAGCCAC 
GTGGAGCCAG TATATATGCA ACAAATTGGA AATGCTAAGA TATCACTAAG CTCGTCTGGA 
GTTTTGTTGA ATGGAAGCTT AGTACCTGCA AGTACTTCAC CTGTCTTCCT TAATGGCAAT 
TCTTTTATTC AGGGACACAA TGGAGTTATC CTTAATGGAC TGAATGTGGG AAATACACAG 
ACAGTGTCAC TGAACCCACC CAAAATGTCT TCAAACATTG TGGGCAATGG CATAGCCATG 
ACAGACATCC TGGGATCTAC CTCCCAGGAT GTGAAAGAAT TCAAAGTCCT CCAGAGTTCT 
GCTGTGAACT CAGCAGCCAC CACCTCCTAC AGCCCTAGTG CCCCTGTGTC ATTCCCAGGG 
CTGATACCCT GCACTGAAGT GAAAAGAGAA GGCATTCAGA CAGTGGCCTC CCAGGACGGT 
GGCTCTGTGG TGACTTTTAC TACACCCGTG CAAATTAACC AGTATGGCAT TGTCCAGATC 
CCTAATTCTG GAGCCAACGG CCAGTTCCTT AATGGGAGCA TTGGATTCTC TCCACTGCAG 
CTGCCTCCTG TCTCAGTAGC AGCTTCACAA GGTAATCTTT CAGTTACCCC AAGCACTTCG 
GACGGAAGCA CATTTACAAG TGAGCCAGCC ACAGTCCAGC ACGGTAAACT TTTCCTGAGC 
CCTCTTACCC CCAGTGCAGT GGTATACACC GTTCCTAATT CAGGCCAGAC TGTAGGAGCT 
GTGAAACAGG AAGGCTTAGA AAGAGGCCTG GTCTTTTCTC AGTTGATGCC TGTCAATCAC 
AGTGCACAAG TAAATGCAAG CCTGTCTTCG GAAAATCTCT CTGGGAGTGG CCTCCATCCT 
CTGACCTCGT CGTTGGTTAA TGTGTCCGCC GCCCACGGTT TTTCCCTGAC CCCCCCTACA 
CTACTTAATC CCACTGAGCT CAACCCTGAC CTTGCCGAGA GCCAGCCCGT GTCTGCACCT 
GTGGCAAGCA AATGTACTGT GTCATCTGTC AGCAACACTA ACTATGCAAC CCTTCAGAAC 
TGTTCCCTTA TTCCGGGGCA AGACCTATTG TCCGGCCCGA TGACCCAGGC TGCCCTGGGT 
GAGATCGTAC CCACAGCTGA AGAGCAGGTG AGCCACGCCT CAACAGCAGT CCACCAGGAT 
TTTGTCAGAG AACAGCGCTT GGTTCTGCAG TCAGTCCCTA ACATAAAAGA GAATTTCTTA 
CAAAATTCTG AGAACAAAGC AACAAACAAC TTAATGATGT TAGACTCGAA ATCCAAGTAC 
GTCCTGGACG GCATGGTTGA AGCCGGCTGT GAAGACCTGG GAACGGACAA AAAAGAGCTG 
GCCAAGCTCC AAACCGTCCA GTTGGATGAA GACATGCAAG ACTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035512.1
CDS	62..2389
Translation	MSSSSPTGQIASAADIKQENGMESASEGQEAHREVAGGAAAGLSPPAPAPFPLEPGDAAAASRVSREEGAAAAGAADQVQLHSELLGRHQHAAAAQPPLAFSPDHVACVCEALQQGGNLDRLARFLWSLPQSDLLRGNESLLKARALVAFHQGIYPELYSILESHSFESANHPLLQQLWYKARYTEAERARGRPLGAVDKYRLRRKFPLPRTIWDGEETVYCFKEKSRNALKELYKQNRYPSPAEKRHLAKITGLSLTQVSNWFKNRRQRDRNPSETQSKSESDGNPSTEDESSKGHEDLSPHPLSGASDGVTNLSLSSHVEPVYMQQIGNAKISLSSSGVLLNGSLVPASTSPVFLNGNSFIQGHNGVILNGLNVGNTQTVSLNPPKMSSNIVGNGIAMTDILGSTSQDVKEFKVLQSSAVNSAATTSYSPSAPVSFPGLIPCTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANGQFLNGSIGFSPLQLPPVSVAASQGNLSVTPSTSDGSTFTSEPATVQHGKLFLSPLTPSAVVYTVPNSGQTVGAVKQEGLERGLVFSQLMPVNHSAQVNASLSSENLSGSGLHPLTSSLVNVSAAHGFSLTPPTLLNPTELNPDLAESQPVSAPVASKCTVSSVSNTNYATLQNCSLIPGQDLLSGPMTQAALGEIVPTAEEQVSHASTAVHQDFVREQRLVLQSVPNIKENFLQNSENKATNNLMMLDSKSKYVLDGMVEAGCEDLGTDKKELAKLQTVQLDEDMQDL

Target ORF information:

RefSeq Version	NM_011382.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sine oculis-related homeobox 4 (Six4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011382.2

ORF Insert Sequence:

ATGTCCTCTT CCTCCCCCAC CGGGCAGATT GCAAGTGCGG CGGACATCAA GCAGGAGAAT 
GGGATGGAAA GCGCCTCGGA AGGACAGGAG GCGCACCGAG AAGTGGCGGG GGGCGCGGCG 
GCTGGGCTGA GCCCCCCGGC TCCAGCTCCT TTCCCCCTGG AGCCGGGGGA CGCCGCGGCT 
GCCTCCAGGG TAAGCCGGGA GGAAGGGGCA GCGGCGGCGG GAGCGGCAGA TCAGGTACAA 
CTCCACTCGG AACTTCTGGG CAGGCACCAG CACGCAGCCG CCGCGCAGCC CCCACTGGCC 
TTCTCGCCGG ACCATGTCGC CTGCGTGTGC GAGGCGTTGC AGCAGGGGGG CAACCTGGAC 
CGCCTGGCCC GGTTTCTTTG GTCCCTGCCC CAGAGCGATC TGCTACGTGG CAACGAGAGC 
CTTCTGAAGG CGCGAGCGCT CGTGGCCTTC CACCAGGGCA TCTACCCTGA GTTGTACAGC 
ATCCTCGAGA GCCACAGCTT CGAGTCGGCC AACCATCCGC TGCTGCAGCA GCTCTGGTAC 
AAGGCGCGCT ACACCGAGGC CGAGCGAGCG CGCGGCCGGC CGCTGGGCGC CGTGGACAAG 
TACCGGCTGC GCAGGAAATT CCCCTTGCCC CGCACCATCT GGGACGGCGA GGAGACGGTG 
TATTGTTTCA AGGAGAAGTC GCGCAACGCG CTCAAGGAGC TCTACAAGCA GAATCGCTAC 
CCCTCGCCGG CTGAGAAGCG GCACCTGGCC AAGATCACCG GCCTCTCCCT CACCCAGGTC 
AGCAACTGGT TCAAGAACCG GCGGCAGCGT GACCGAAACC CCTCCGAGAC CCAGTCCAAA 
AGCGAATCGG ATGGCAACCC CAGTACCGAG GATGAATCCA GCAAGGGACA TGAGGATTTG 
TCTCCTCATC CACTTTCAGG CGCATCTGAT GGCGTCACCA ACCTCAGCCT CTCTAGCCAC 
GTGGAGCCAG TATATATGCA ACAAATTGGA AATGCTAAGA TATCACTAAG CTCGTCTGGA 
GTTTTGTTGA ATGGAAGCTT AGTACCTGCA AGTACTTCAC CTGTCTTCCT TAATGGCAAT 
TCTTTTATTC AGGGACACAA TGGAGTTATC CTTAATGGAC TGAATGTGGG AAATACACAG 
ACAGTGTCAC TGAACCCACC CAAAATGTCT TCAAACATTG TGGGCAATGG CATAGCCATG 
ACAGACATCC TGGGATCTAC CTCCCAGGAT GTGAAAGAAT TCAAAGTCCT CCAGAGTTCT 
GCTGTGAACT CAGCAGCCAC CACCTCCTAC AGCCCTAGTG CCCCTGTGTC ATTCCCAGGG 
CTGATACCCT GCACTGAAGT GAAAAGAGAA GGCATTCAGA CAGTGGCCTC CCAGGACGGT 
GGCTCTGTGG TGACTTTTAC TACACCCGTG CAAATTAACC AGTATGGCAT TGTCCAGATC 
CCTAATTCTG GAGCCAACGG CCAGTTCCTT AATGGGAGCA TTGGATTCTC TCCACTGCAG 
CTGCCTCCTG TCTCAGTAGC AGCTTCACAA GGTAATCTTT CAGTTACCCC AAGCACTTCG 
GACGGAAGCA CATTTACAAG TGAGCCAGCC ACAGTCCAGC ACGGTAAACT TTTCCTGAGC 
CCTCTTACCC CCAGTGCAGT GGTATACACC GTTCCTAATT CAGGCCAGAC TGTAGGAGCT 
GTGAAACAGG AAGGCTTAGA AAGAGGCCTG GTCTTTTCTC AGTTGATGCC TGTCAATCAC 
AGTGCACAAG TAAATGCAAG CCTGTCTTCG GAAAATCTCT CTGGGAGTGG CCTCCATCCT 
CTGACCTCGT CGTTGGTTAA TGTGTCCGCC GCCCACGGTT TTTCCCTGAC CCCCCCTACA 
CTACTTAATC CCACTGAGCT CAACCCTGAC CTTGCCGAGA GCCAGCCCGT GTCTGCACCT 
GTGGCAAGCA AATGTACTGT GTCATCTGTC AGCAACACTA ACTATGCAAC CCTTCAGAAC 
TGTTCCCTTA TTCCGGGGCA AGACCTATTG TCCGGCCCGA TGACCCAGGC TGCCCTGGGT 
GAGATCGTAC CCACAGCTGA AGAGCAGGTG AGCCACGCCT CAACAGCAGT CCACCAGGAT 
TTTGTCAGAG AACAGCGCTT GGTTCTGCAG TCAGTCCCTA ACATAAAAGA GAATTTCTTA 
CAAAATTCTG AGAACAAAGC AACAAACAAC TTAATGATGT TAGACTCGAA ATCCAAGTAC 
GTCCTGGACG GCATGGTTGA AGCCGGCTGT GAAGACCTGG GAACGGACAA AAAAGAGCTG 
GCCAAGCTCC AAACCGTCCA GTTGGATGAA GACATGCAAG ACTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Six4 ( NM_001362272.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349201.1 Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant 2, mRNA.

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CloneID	OMu84251
Clone ID Related Accession (Same CDS sequence)	NM_001362272.1
Accession Version	NM_001362272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	homeobox protein SIX4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ180841.1, D50417.1 and AC159274.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATTGCAA GTGCGGCGGA CATCAAGCAG GAGAATGGGA TGGAAAGCGC CTCGGAAGGA 
CAGGAGGCGC ACCGAGAAGT GGCGGGGGGC GCGGCGGCTG GGCTGAGCCC CCCGGCTCCA 
GCTCCTTTCC CCCTGGAGCC GGGGGACGCC GCGGCTGCCT CCAGGGTAAG CCGGGAGGAA 
GGGGCAGCGG CGGCGGGAGC GGCAGATCAG GTACAACTCC ACTCGGAACT TCTGGGCAGG 
CACCAGCACG CAGCCGCCGC GCAGCCCCCA CTGGCCTTCT CGCCGGACCA TGTCGCCTGC 
GTGTGCGAGG CGTTGCAGCA GGGGGGCAAC CTGGACCGCC TGGCCCGGTT TCTTTGGTCC 
CTGCCCCAGA GCGATCTGCT ACGTGGCAAC GAGAGCCTTC TGAAGGCGCG AGCGCTCGTG 
GCCTTCCACC AGGGCATCTA CCCTGAGTTG TACAGCATCC TCGAGAGCCA CAGCTTCGAG 
TCGGCCAACC ATCCGCTGCT GCAGCAGCTC TGGTACAAGG CGCGCTACAC CGAGGCCGAG 
CGAGCGCGCG GCCGGCCGCT GGGCGCCGTG GACAAGTACC GGCTGCGCAG GAAATTCCCC 
TTGCCCCGCA CCATCTGGGA CGGCGAGGAG ACGGTGTATT GTTTCAAGGA GAAGTCGCGC 
AACGCGCTCA AGGAGCTCTA CAAGCAGAAT CGCTACCCCT CGCCGGCTGA GAAGCGGCAC 
CTGGCCAAGA TCACCGGCCT CTCCCTCACC CAGGTCAGCA ACTGGTTCAA GAACCGGCGG 
CAGCGTGACC GAAACCCCTC CGAGACCCAG TCCAAAAGCG AATCGGATGG CAACCCCAGT 
ACCGAGGATG AATCCAGCAA GGGACATGAG GATTTGTCTC CTCATCCACT TTCAGGCGCA 
TCTGATGGCG TCACCAACCT CAGCCTCTCT AGCCACGTGG AGCCAGTATA TATGCAACAA 
ATTGGAAATG CTAAGATATC ACTAAGCTCG TCTGGAGTTT TGTTGAATGG AAGCTTAGTA 
CCTGCAAGTA CTTCACCTGT CTTCCTTAAT GGCAATTCTT TTATTCAGGG ACACAATGGA 
GTTATCCTTA ATGGACTGAA TGTGGGAAAT ACACAGACAG TGTCACTGAA CCCACCCAAA 
ATGTCTTCAA ACATTGTGGG CAATGGCATA GCCATGACAG ACATCCTGGG ATCTACCTCC 
CAGGATGTGA AAGAATTCAA AGTCCTCCAG AGTTCTGCTG TGAACTCAGC AGCCACCACC 
TCCTACAGCC CTAGTGCCCC TGTGTCATTC CCAGGGCTGA TACCCTGCAC TGAAGTGAAA 
AGAGAAGGCA TTCAGACAGT GGCCTCCCAG GACGGTGGCT CTGTGGTGAC TTTTACTACA 
CCCGTGCAAA TTAACCAGTA TGGCATTGTC CAGATCCCTA ATTCTGGAGC CAACGGCCAG 
TTCCTTAATG GGAGCATTGG ATTCTCTCCA CTGCAGCTGC CTCCTGTCTC AGTAGCAGCT 
TCACAAGGTA AAAGCTTCAT TTGGTACCAG CAATGCACCA GGGCATGTGT TGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349201.1
CDS	60..1616
Translation	MIASAADIKQENGMESASEGQEAHREVAGGAAAGLSPPAPAPFPLEPGDAAAASRVSREEGAAAAGAADQVQLHSELLGRHQHAAAAQPPLAFSPDHVACVCEALQQGGNLDRLARFLWSLPQSDLLRGNESLLKARALVAFHQGIYPELYSILESHSFESANHPLLQQLWYKARYTEAERARGRPLGAVDKYRLRRKFPLPRTIWDGEETVYCFKEKSRNALKELYKQNRYPSPAEKRHLAKITGLSLTQVSNWFKNRRQRDRNPSETQSKSESDGNPSTEDESSKGHEDLSPHPLSGASDGVTNLSLSSHVEPVYMQQIGNAKISLSSSGVLLNGSLVPASTSPVFLNGNSFIQGHNGVILNGLNVGNTQTVSLNPPKMSSNIVGNGIAMTDILGSTSQDVKEFKVLQSSAVNSAATTSYSPSAPVSFPGLIPCTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANGQFLNGSIGFSPLQLPPVSVAASQGKSFIWYQQCTRACVG

Target ORF information:

RefSeq Version	NM_001362272.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362272.1

ORF Insert Sequence:

ATGATTGCAA GTGCGGCGGA CATCAAGCAG GAGAATGGGA TGGAAAGCGC CTCGGAAGGA 
CAGGAGGCGC ACCGAGAAGT GGCGGGGGGC GCGGCGGCTG GGCTGAGCCC CCCGGCTCCA 
GCTCCTTTCC CCCTGGAGCC GGGGGACGCC GCGGCTGCCT CCAGGGTAAG CCGGGAGGAA 
GGGGCAGCGG CGGCGGGAGC GGCAGATCAG GTACAACTCC ACTCGGAACT TCTGGGCAGG 
CACCAGCACG CAGCCGCCGC GCAGCCCCCA CTGGCCTTCT CGCCGGACCA TGTCGCCTGC 
GTGTGCGAGG CGTTGCAGCA GGGGGGCAAC CTGGACCGCC TGGCCCGGTT TCTTTGGTCC 
CTGCCCCAGA GCGATCTGCT ACGTGGCAAC GAGAGCCTTC TGAAGGCGCG AGCGCTCGTG 
GCCTTCCACC AGGGCATCTA CCCTGAGTTG TACAGCATCC TCGAGAGCCA CAGCTTCGAG 
TCGGCCAACC ATCCGCTGCT GCAGCAGCTC TGGTACAAGG CGCGCTACAC CGAGGCCGAG 
CGAGCGCGCG GCCGGCCGCT GGGCGCCGTG GACAAGTACC GGCTGCGCAG GAAATTCCCC 
TTGCCCCGCA CCATCTGGGA CGGCGAGGAG ACGGTGTATT GTTTCAAGGA GAAGTCGCGC 
AACGCGCTCA AGGAGCTCTA CAAGCAGAAT CGCTACCCCT CGCCGGCTGA GAAGCGGCAC 
CTGGCCAAGA TCACCGGCCT CTCCCTCACC CAGGTCAGCA ACTGGTTCAA GAACCGGCGG 
CAGCGTGACC GAAACCCCTC CGAGACCCAG TCCAAAAGCG AATCGGATGG CAACCCCAGT 
ACCGAGGATG AATCCAGCAA GGGACATGAG GATTTGTCTC CTCATCCACT TTCAGGCGCA 
TCTGATGGCG TCACCAACCT CAGCCTCTCT AGCCACGTGG AGCCAGTATA TATGCAACAA 
ATTGGAAATG CTAAGATATC ACTAAGCTCG TCTGGAGTTT TGTTGAATGG AAGCTTAGTA 
CCTGCAAGTA CTTCACCTGT CTTCCTTAAT GGCAATTCTT TTATTCAGGG ACACAATGGA 
GTTATCCTTA ATGGACTGAA TGTGGGAAAT ACACAGACAG TGTCACTGAA CCCACCCAAA 
ATGTCTTCAA ACATTGTGGG CAATGGCATA GCCATGACAG ACATCCTGGG ATCTACCTCC 
CAGGATGTGA AAGAATTCAA AGTCCTCCAG AGTTCTGCTG TGAACTCAGC AGCCACCACC 
TCCTACAGCC CTAGTGCCCC TGTGTCATTC CCAGGGCTGA TACCCTGCAC TGAAGTGAAA 
AGAGAAGGCA TTCAGACAGT GGCCTCCCAG GACGGTGGCT CTGTGGTGAC TTTTACTACA 
CCCGTGCAAA TTAACCAGTA TGGCATTGTC CAGATCCCTA ATTCTGGAGC CAACGGCCAG 
TTCCTTAATG GGAGCATTGG ATTCTCTCCA CTGCAGCTGC CTCCTGTCTC AGTAGCAGCT 
TCACAAGGTA AAAGCTTCAT TTGGTACCAG CAATGCACCA GGGCATGTGT TGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Six4 ( XM_006515632.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515695.1 Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant X1, mRNA.

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CloneID	OMu47690
Clone ID Related Accession (Same CDS sequence)	XM_006515632.4 , XM_006515632.3
Accession Version	XM_006515632.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	homeobox protein SIX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515632.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCAA GTGCGGCGGA CATCAAGCAG GAGAATGGGA TGGAAAGCGC CTCGGAAGGA 
CAGGAGGCGC ACCGAGAAGT GGCGGGGGGC GCGGCGGCTG GGCTGAGCCC CCCGGCTCCA 
GCTCCTTTCC CCCTGGAGCC GGGGGACGCC GCGGCTGCCT CCAGGGTAAG CCGGGAGGAA 
GGGGCAGCGG CGGCGGGAGC GGCAGATCAG GTACAACTCC ACTCGGAACT TCTGGGCAGG 
CACCAGCACG CAGCCGCCGC GCAGCCCCCA CTGGCCTTCT CGCCGGACCA TGTCGCCTGC 
GTGTGCGAGG CGTTGCAGCA GGGGGGCAAC CTGGACCGCC TGGCCCGGTT TCTTTGGTCC 
CTGCCCCAGA GCGATCTGCT ACGTGGCAAC GAGAGCCTTC TGAAGGCGCG AGCGCTCGTG 
GCCTTCCACC AGGGCATCTA CCCTGAGTTG TACAGCATCC TCGAGAGCCA CAGCTTCGAG 
TCGGCCAACC ATCCGCTGCT GCAGCAGCTC TGGTACAAGG CGCGCTACAC CGAGGCCGAG 
CGAGCGCGCG GCCGGCCGCT GGGCGCCGTG GACAAGTACC GGCTGCGCAG GAAATTCCCC 
TTGCCCCGCA CCATCTGGGA CGGCGAGGAG ACGGTGTATT GTTTCAAGGA GAAGTCGCGC 
AACGCGCTCA AGGAGCTCTA CAAGCAGAAT CGCTACCCCT CGCCGGCTGA GAAGCGGCAC 
CTGGCCAAGA TCACCGGCCT CTCCCTCACC CAGGTCAGCA ACTGGTTCAA GAACCGGCGG 
CAGCGTGACC GAAACCCCTC CGAGACCCAG TCCAAAAGCG AATCGGATGG CAACCCCAGT 
ACCGAGGATG AATCCAGCAA GGGACATGAG GATTTGTCTC CTCATCCACT TTCAGGCGCA 
TCTGATGGCG TCACCAACCT CAGCCTCTCT AGCCACGTGG AGCCAGTATA TATGCAACAA 
ATTGGAAATG CTAAGATATC ACTAAGCTCG TCTGGAGTTT TGTTGAATGG AAGCTTAGTA 
CCTGCAAGTA CTTCACCTGT CTTCCTTAAT GGCAATTCTT TTATTCAGGG ACACAATGGA 
GTTATCCTTA ATGGACTGAA TGTGGGAAAT ACACAGACAG TGTCACTGAA CCCACCCAAA 
ATGTCTTCAA ACATTGTGGG CAATGGCATA GCCATGACAG ACATCCTGGG ATCTACCTCC 
CAGGATGTGA AAGAATTCAA AGTCCTCCAG AGTTCTGCTG TGAACTCAGC AGCCACCACC 
TCCTACAGCC CTAGTGCCCC TGTGTCATTC CCAGGGCTGA TACCCTGCAC TGAAGTGAAA 
AGAGAAGGCA TTCAGACAGT GGCCTCCCAG GACGGTGGCT CTGTGGTGAC TTTTACTACA 
CCCGTGCAAA TTAACCAGTA TGGCATTGTC CAGATCCCTA ATTCTGGAGC CAACGGCCAG 
TTCCTTAATG GGAGCATTGG ATTCTCTCCA CTGCAGCTGC CTCCTGTCTC AGTAGCAGCT 
TCACAAGGTA ATCTTTCAGT TACCCCAAGC ACTTCGGACG GAAGCACATT TACAAGTGAG 
CCAGCCACAG TCCAGCACGG TAAACTTTTC CTGAGCCCTC TTACCCCCAG TGCAGTGGTA 
TACACCGTTC CTAATTCAGG CCAGACTGTA GGAGCTGTGA AACAGGAAGG CTTAGAAAGA 
GGCCTGGTCT TTTCTCAGTT GATGCCTGTC AATCACAGTG CACAAGTAAA TGCAAGCCTG 
TCTTCGGAAA ATCTCTCTGG GAGTGGCCTC CATCCTCTGA CCTCGTCGTT GGTTAATGTG 
TCCGCCGCCC ACGGTTTTTC CCTGACCCCC CCTACACTAC TTAATCCCAC TGAGCTCAAC 
CCTGACCTTG CCGAGAGCCA GCCCGTGTCT GCACCTGTGG CAAGCAAATG TACTGTGTCA 
TCTGTCAGCA ACACTAACTA TGCAACCCTT CAGAACTGTT CCCTTATTCC GGGGCAAGAC 
CTATTGTCCG GCCCGATGAC CCAGGCTGCC CTGGGTGAGA TCGTACCCAC AGCTGAAGAG 
CAGGTGAGCC ACGCCTCAAC AGCAGTCCAC CAGGATTTTG TCAGAGAACA GCGCTTGGTT 
CTGCAGTCAG TCCCTAACAT AAAAGAGAAT TTCTTACAAA ATTCTGAGAA CAAAGCAACA 
AACAACTTAA TGATGTTAGA CTCGAAATCC AAGTACGTCC TGGACGGCAT GGTTGAAGCC 
GGCTGTGAAG ACCTGGGAAC GGACAAAAAA GAGCTGGCCA AGCTCCAAAC CGTCCAGTTG 
GATGAAGACA TGCAAGACTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515695.1
CDS	666..2969
Translation	MIASAADIKQENGMESASEGQEAHREVAGGAAAGLSPPAPAPFPLEPGDAAAASRVSREEGAAAAGAADQVQLHSELLGRHQHAAAAQPPLAFSPDHVACVCEALQQGGNLDRLARFLWSLPQSDLLRGNESLLKARALVAFHQGIYPELYSILESHSFESANHPLLQQLWYKARYTEAERARGRPLGAVDKYRLRRKFPLPRTIWDGEETVYCFKEKSRNALKELYKQNRYPSPAEKRHLAKITGLSLTQVSNWFKNRRQRDRNPSETQSKSESDGNPSTEDESSKGHEDLSPHPLSGASDGVTNLSLSSHVEPVYMQQIGNAKISLSSSGVLLNGSLVPASTSPVFLNGNSFIQGHNGVILNGLNVGNTQTVSLNPPKMSSNIVGNGIAMTDILGSTSQDVKEFKVLQSSAVNSAATTSYSPSAPVSFPGLIPCTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANGQFLNGSIGFSPLQLPPVSVAASQGNLSVTPSTSDGSTFTSEPATVQHGKLFLSPLTPSAVVYTVPNSGQTVGAVKQEGLERGLVFSQLMPVNHSAQVNASLSSENLSGSGLHPLTSSLVNVSAAHGFSLTPPTLLNPTELNPDLAESQPVSAPVASKCTVSSVSNTNYATLQNCSLIPGQDLLSGPMTQAALGEIVPTAEEQVSHASTAVHQDFVREQRLVLQSVPNIKENFLQNSENKATNNLMMLDSKSKYVLDGMVEAGCEDLGTDKKELAKLQTVQLDEDMQDL

Target ORF information:

RefSeq Version	XM_006515632.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sine oculis-related homeobox 4 (Six4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515632.4

ORF Insert Sequence:

ATGATTGCAA GTGCGGCGGA CATCAAGCAG GAGAATGGGA TGGAAAGCGC CTCGGAAGGA 
CAGGAGGCGC ACCGAGAAGT GGCGGGGGGC GCGGCGGCTG GGCTGAGCCC CCCGGCTCCA 
GCTCCTTTCC CCCTGGAGCC GGGGGACGCC GCGGCTGCCT CCAGGGTAAG CCGGGAGGAA 
GGGGCAGCGG CGGCGGGAGC GGCAGATCAG GTACAACTCC ACTCGGAACT TCTGGGCAGG 
CACCAGCACG CAGCCGCCGC GCAGCCCCCA CTGGCCTTCT CGCCGGACCA TGTCGCCTGC 
GTGTGCGAGG CGTTGCAGCA GGGGGGCAAC CTGGACCGCC TGGCCCGGTT TCTTTGGTCC 
CTGCCCCAGA GCGATCTGCT ACGTGGCAAC GAGAGCCTTC TGAAGGCGCG AGCGCTCGTG 
GCCTTCCACC AGGGCATCTA CCCTGAGTTG TACAGCATCC TCGAGAGCCA CAGCTTCGAG 
TCGGCCAACC ATCCGCTGCT GCAGCAGCTC TGGTACAAGG CGCGCTACAC CGAGGCCGAG 
CGAGCGCGCG GCCGGCCGCT GGGCGCCGTG GACAAGTACC GGCTGCGCAG GAAATTCCCC 
TTGCCCCGCA CCATCTGGGA CGGCGAGGAG ACGGTGTATT GTTTCAAGGA GAAGTCGCGC 
AACGCGCTCA AGGAGCTCTA CAAGCAGAAT CGCTACCCCT CGCCGGCTGA GAAGCGGCAC 
CTGGCCAAGA TCACCGGCCT CTCCCTCACC CAGGTCAGCA ACTGGTTCAA GAACCGGCGG 
CAGCGTGACC GAAACCCCTC CGAGACCCAG TCCAAAAGCG AATCGGATGG CAACCCCAGT 
ACCGAGGATG AATCCAGCAA GGGACATGAG GATTTGTCTC CTCATCCACT TTCAGGCGCA 
TCTGATGGCG TCACCAACCT CAGCCTCTCT AGCCACGTGG AGCCAGTATA TATGCAACAA 
ATTGGAAATG CTAAGATATC ACTAAGCTCG TCTGGAGTTT TGTTGAATGG AAGCTTAGTA 
CCTGCAAGTA CTTCACCTGT CTTCCTTAAT GGCAATTCTT TTATTCAGGG ACACAATGGA 
GTTATCCTTA ATGGACTGAA TGTGGGAAAT ACACAGACAG TGTCACTGAA CCCACCCAAA 
ATGTCTTCAA ACATTGTGGG CAATGGCATA GCCATGACAG ACATCCTGGG ATCTACCTCC 
CAGGATGTGA AAGAATTCAA AGTCCTCCAG AGTTCTGCTG TGAACTCAGC AGCCACCACC 
TCCTACAGCC CTAGTGCCCC TGTGTCATTC CCAGGGCTGA TACCCTGCAC TGAAGTGAAA 
AGAGAAGGCA TTCAGACAGT GGCCTCCCAG GACGGTGGCT CTGTGGTGAC TTTTACTACA 
CCCGTGCAAA TTAACCAGTA TGGCATTGTC CAGATCCCTA ATTCTGGAGC CAACGGCCAG 
TTCCTTAATG GGAGCATTGG ATTCTCTCCA CTGCAGCTGC CTCCTGTCTC AGTAGCAGCT 
TCACAAGGTA ATCTTTCAGT TACCCCAAGC ACTTCGGACG GAAGCACATT TACAAGTGAG 
CCAGCCACAG TCCAGCACGG TAAACTTTTC CTGAGCCCTC TTACCCCCAG TGCAGTGGTA 
TACACCGTTC CTAATTCAGG CCAGACTGTA GGAGCTGTGA AACAGGAAGG CTTAGAAAGA 
GGCCTGGTCT TTTCTCAGTT GATGCCTGTC AATCACAGTG CACAAGTAAA TGCAAGCCTG 
TCTTCGGAAA ATCTCTCTGG GAGTGGCCTC CATCCTCTGA CCTCGTCGTT GGTTAATGTG 
TCCGCCGCCC ACGGTTTTTC CCTGACCCCC CCTACACTAC TTAATCCCAC TGAGCTCAAC 
CCTGACCTTG CCGAGAGCCA GCCCGTGTCT GCACCTGTGG CAAGCAAATG TACTGTGTCA 
TCTGTCAGCA ACACTAACTA TGCAACCCTT CAGAACTGTT CCCTTATTCC GGGGCAAGAC 
CTATTGTCCG GCCCGATGAC CCAGGCTGCC CTGGGTGAGA TCGTACCCAC AGCTGAAGAG 
CAGGTGAGCC ACGCCTCAAC AGCAGTCCAC CAGGATTTTG TCAGAGAACA GCGCTTGGTT 
CTGCAGTCAG TCCCTAACAT AAAAGAGAAT TTCTTACAAA ATTCTGAGAA CAAAGCAACA 
AACAACTTAA TGATGTTAGA CTCGAAATCC AAGTACGTCC TGGACGGCAT GGTTGAAGCC 
GGCTGTGAAG ACCTGGGAAC GGACAAAAAA GAGCTGGCCA AGCTCCAAAC CGTCCAGTTG 
GATGAAGACA TGCAAGACTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Specification of jaw identity by the Hand2 transcription factor.
Scientific reports628405(2016 Jun)
Funato N,Kokubo H,Nakamura M,Yanagisawa H,Saga Y

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Six4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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