Itsn2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
NM_001198968.2	NP_001185897.1	intersectin-2 isoform 1
NM_001198969.2	NP_001185898.1	intersectin-2 isoform 3
XM_006515024.3	XP_006515087.1	intersectin-2 isoform X1
XM_030246627.1	XP_030102487.1	intersectin-2 isoform X1
XM_006515025.4	XP_006515088.1	intersectin-2 isoform X2
XM_006515026.4	XP_006515089.1	intersectin-2 isoform X3
XM_006515023.3	XP_006515086.1	intersectin-2 isoform X1
XM_006515023.4	XP_006515086.1	intersectin-2 isoform X1
XM_006515025.3	XP_006515088.1	intersectin-2 isoform X2
XM_006515026.3	XP_006515089.1	intersectin-2 isoform X3
XM_006515027.3	XP_006515090.1	intersectin-2 isoform X4
XM_006515024.4	XP_006515087.1	intersectin-2 isoform X1
XM_006515027.4	XP_006515090.1	intersectin-2 isoform X4
NM_011365.4	NP_035495.2	intersectin-2 isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01937	NM_001198969.2 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu47269	XM_006515026.4 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu47270	XM_006515027.4 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu01935	XM_006515023.4 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu01935	XM_030246627.1 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu01935	XM_006515024.4 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu47268	XM_006515025.4 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu01935	NM_001198968.2 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu08005	NM_011365.4 Latest version!	Mus musculus intersectin 2 (Itsn2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$972.30 $1389.00
View Old Accession Versions >>
OMu01935	XM_006515023.3	Mus musculus intersectin 2 (Itsn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu47268	XM_006515025.3	Mus musculus intersectin 2 (Itsn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu47269	XM_006515026.3	Mus musculus intersectin 2 (Itsn2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu01935	XM_006515024.3	Mus musculus intersectin 2 (Itsn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu47270	XM_006515027.3	Mus musculus intersectin 2 (Itsn2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

Itsn2 ( NM_001198969.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185898.1 Mus musculus intersectin 2 (Itsn2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu01937
Clone ID Related Accession (Same CDS sequence)	NM_001198969.2
Accession Version	NM_001198969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC241534.2, BC138262.1 and AK031390.1. On Mar 7, 2012 this sequence version replaced NM_001198969.1. Transcript Variant: This variant (3) lacks multiple 3' exons and has a novel 3' terminus, compared to variant 1. It encodes an isoform (3) with a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK034367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGTT ATTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185898.1
CDS	258..2216
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKVIF

Target ORF information:

RefSeq Version	NM_001198969.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198969.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGTT ATTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515026.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515089.1 Mus musculus intersectin 2 (Itsn2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu47269
Clone ID Related Accession (Same CDS sequence)	XM_006515026.4 , XM_006515026.3
Accession Version	XM_006515026.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515026.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515089.1
CDS	349..3891
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	XM_006515026.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515026.4

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515027.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515090.1 Mus musculus intersectin 2 (Itsn2), transcript variant X6, mRNA.

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CloneID	OMu47270
Clone ID Related Accession (Same CDS sequence)	XM_006515027.3 , XM_006515027.4
Accession Version	XM_006515027.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515027.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGGAAC TCAGAGAAAG CTATAATACA CAGCAGTTAG CCCTTGAACA ACTTCATAAA 
ATCAAACGTG ACAAATTGAA GGAAATCGAA AGAAAAAGAT TAGAGCAAAT TCAAAAAAAG 
AAACTAGAAG ATGAGGCTGC AAGGAAAGCA AAGCAAGGAA AAGAAAACTT GTGGAGAGAA 
AGTATTAGAA AGGAAGAAGA GGAAAAGCAA AAACGACTCC AGGAAGAAAA GTCACAGGAC 
AAAACTCAAG AAGAGGAACG AAAAGCTGAG GCAAAACAAA GTGAGACAGC CAGTGCTTTG 
GTGAATTACA GAGCACTGTA CCCTTTTGAA GCAAGAAACC ATGATGAGAT GAGTTTTAGT 
TCTGGGGATA TAATTCAGGT TGATGAAAAA ACTGTAGGAG AGCCTGGTTG GCTTTATGGT 
AGTTTTCAGG GAAAGTTTGG CTGGTTCCCG TGCAACTATG TAGAAAAAGT GCTGTCAAGT 
GAAAAAGCTC TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTGTCTCT CTCTGCTACC 
TCAACTTCTT CCCAGCCACC AGCATCAGTG ACTGATTATC ACAATGTATC CTTCTCAAAC 
CTTACTGTTA ATACAACATG GCAGCAGAAG TCAGCTTTTA CCCGCACTGT GTCCCCTGGA 
TCTGTGTCCC CCATTCACGG ACAGGGGCAG GCTGTAGAAA ACCTGAAAGC CCAGGCCCTT 
TGTTCCTGGA CGGCAAAGAA GGAGAACCAC CTGAACTTCT CAAAGCACGA CGTCATCACT 
GTCCTGGAGC AGCAGGAAAA CTGGTGGTTT GGGGAGGTGC ACGGAGGAAG AGGATGGTTC 
CCCAAGTCTT ATGTCAAGCT CATTCCTGGG AATGAAGTAC AGCGAGGAGA GCCAGAAGCT 
TTGTATGCAG CTGTGACTAA GAAACCTACC TCCACAGCCT ATCCAGTTAC CTCCACAGCC 
TATCCAGTTG GAGAAGACTA CATTGCACTT TATTCATACT CAAGTGTAGA GCCCGGGGAT 
TTGACTTTCA CTGAAGGTGA AGAAATTCTA GTGACCCAGA AAGATGGAGA GTGGTGGACA 
GGAAGTATTG GAGAGAGAAC TGGAATCTTC CCGTCCAACT ACGTCAGACC AAAGGATCAA 
GAGAATTTTG GGAATGCTAG CAAATCTGGA GCATCAAACA AAAAACCCGA GATCGCTCAA 
GTAACTTCAG CATATGCTGC TTCAGGGACT GAGCAGCTCA GCCTTGCGCC AGGACAGTTA 
ATATTAATCT TAAAGAAAAA CACAAGCGGG TGGTGGCAAG GAGAGCTACA GGCCAGAGGG 
AAGAAACGAC AGAAGGGATG GTTTCCTGCC AGCCATGTAA AGCTGCTAGG TCCAAGCAGT 
GAAAGAACCA TGCCTACTTT TCACGCTGTA TGTCAAGTGA TTGCTATGTA TGACTACATG 
GCGAATAACG AAGATGAGCT CAATTTCTCC AAAGGACAGC TGATTAATGT TATGAACAAA 
GATGACCCTG ACTGGTGGCA AGGAGAAACC AATGGTCTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCAGATCCA AGTCAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515090.1
CDS	349..3810
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	XM_006515027.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515027.4

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGGAAC TCAGAGAAAG CTATAATACA CAGCAGTTAG CCCTTGAACA ACTTCATAAA 
ATCAAACGTG ACAAATTGAA GGAAATCGAA AGAAAAAGAT TAGAGCAAAT TCAAAAAAAG 
AAACTAGAAG ATGAGGCTGC AAGGAAAGCA AAGCAAGGAA AAGAAAACTT GTGGAGAGAA 
AGTATTAGAA AGGAAGAAGA GGAAAAGCAA AAACGACTCC AGGAAGAAAA GTCACAGGAC 
AAAACTCAAG AAGAGGAACG AAAAGCTGAG GCAAAACAAA GTGAGACAGC CAGTGCTTTG 
GTGAATTACA GAGCACTGTA CCCTTTTGAA GCAAGAAACC ATGATGAGAT GAGTTTTAGT 
TCTGGGGATA TAATTCAGGT TGATGAAAAA ACTGTAGGAG AGCCTGGTTG GCTTTATGGT 
AGTTTTCAGG GAAAGTTTGG CTGGTTCCCG TGCAACTATG TAGAAAAAGT GCTGTCAAGT 
GAAAAAGCTC TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTGTCTCT CTCTGCTACC 
TCAACTTCTT CCCAGCCACC AGCATCAGTG ACTGATTATC ACAATGTATC CTTCTCAAAC 
CTTACTGTTA ATACAACATG GCAGCAGAAG TCAGCTTTTA CCCGCACTGT GTCCCCTGGA 
TCTGTGTCCC CCATTCACGG ACAGGGGCAG GCTGTAGAAA ACCTGAAAGC CCAGGCCCTT 
TGTTCCTGGA CGGCAAAGAA GGAGAACCAC CTGAACTTCT CAAAGCACGA CGTCATCACT 
GTCCTGGAGC AGCAGGAAAA CTGGTGGTTT GGGGAGGTGC ACGGAGGAAG AGGATGGTTC 
CCCAAGTCTT ATGTCAAGCT CATTCCTGGG AATGAAGTAC AGCGAGGAGA GCCAGAAGCT 
TTGTATGCAG CTGTGACTAA GAAACCTACC TCCACAGCCT ATCCAGTTAC CTCCACAGCC 
TATCCAGTTG GAGAAGACTA CATTGCACTT TATTCATACT CAAGTGTAGA GCCCGGGGAT 
TTGACTTTCA CTGAAGGTGA AGAAATTCTA GTGACCCAGA AAGATGGAGA GTGGTGGACA 
GGAAGTATTG GAGAGAGAAC TGGAATCTTC CCGTCCAACT ACGTCAGACC AAAGGATCAA 
GAGAATTTTG GGAATGCTAG CAAATCTGGA GCATCAAACA AAAAACCCGA GATCGCTCAA 
GTAACTTCAG CATATGCTGC TTCAGGGACT GAGCAGCTCA GCCTTGCGCC AGGACAGTTA 
ATATTAATCT TAAAGAAAAA CACAAGCGGG TGGTGGCAAG GAGAGCTACA GGCCAGAGGG 
AAGAAACGAC AGAAGGGATG GTTTCCTGCC AGCCATGTAA AGCTGCTAGG TCCAAGCAGT 
GAAAGAACCA TGCCTACTTT TCACGCTGTA TGTCAAGTGA TTGCTATGTA TGACTACATG 
GCGAATAACG AAGATGAGCT CAATTTCTCC AAAGGACAGC TGATTAATGT TATGAACAAA 
GATGACCCTG ACTGGTGGCA AGGAGAAACC AATGGTCTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCAGATCCA AGTCAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515026.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515089.1 Mus musculus intersectin 2 (Itsn2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu47269
Clone ID Related Accession (Same CDS sequence)	XM_006515026.4 , XM_006515026.3
Accession Version	XM_006515026.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515089.1
CDS	227..3769
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	XM_006515026.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515026.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515027.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515090.1 Mus musculus intersectin 2 (Itsn2), transcript variant X5, mRNA.

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CloneID	OMu47270
Clone ID Related Accession (Same CDS sequence)	XM_006515027.3 , XM_006515027.4
Accession Version	XM_006515027.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGGAAC TCAGAGAAAG CTATAATACA CAGCAGTTAG CCCTTGAACA ACTTCATAAA 
ATCAAACGTG ACAAATTGAA GGAAATCGAA AGAAAAAGAT TAGAGCAAAT TCAAAAAAAG 
AAACTAGAAG ATGAGGCTGC AAGGAAAGCA AAGCAAGGAA AAGAAAACTT GTGGAGAGAA 
AGTATTAGAA AGGAAGAAGA GGAAAAGCAA AAACGACTCC AGGAAGAAAA GTCACAGGAC 
AAAACTCAAG AAGAGGAACG AAAAGCTGAG GCAAAACAAA GTGAGACAGC CAGTGCTTTG 
GTGAATTACA GAGCACTGTA CCCTTTTGAA GCAAGAAACC ATGATGAGAT GAGTTTTAGT 
TCTGGGGATA TAATTCAGGT TGATGAAAAA ACTGTAGGAG AGCCTGGTTG GCTTTATGGT 
AGTTTTCAGG GAAAGTTTGG CTGGTTCCCG TGCAACTATG TAGAAAAAGT GCTGTCAAGT 
GAAAAAGCTC TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTGTCTCT CTCTGCTACC 
TCAACTTCTT CCCAGCCACC AGCATCAGTG ACTGATTATC ACAATGTATC CTTCTCAAAC 
CTTACTGTTA ATACAACATG GCAGCAGAAG TCAGCTTTTA CCCGCACTGT GTCCCCTGGA 
TCTGTGTCCC CCATTCACGG ACAGGGGCAG GCTGTAGAAA ACCTGAAAGC CCAGGCCCTT 
TGTTCCTGGA CGGCAAAGAA GGAGAACCAC CTGAACTTCT CAAAGCACGA CGTCATCACT 
GTCCTGGAGC AGCAGGAAAA CTGGTGGTTT GGGGAGGTGC ACGGAGGAAG AGGATGGTTC 
CCCAAGTCTT ATGTCAAGCT CATTCCTGGG AATGAAGTAC AGCGAGGAGA GCCAGAAGCT 
TTGTATGCAG CTGTGACTAA GAAACCTACC TCCACAGCCT ATCCAGTTAC CTCCACAGCC 
TATCCAGTTG GAGAAGACTA CATTGCACTT TATTCATACT CAAGTGTAGA GCCCGGGGAT 
TTGACTTTCA CTGAAGGTGA AGAAATTCTA GTGACCCAGA AAGATGGAGA GTGGTGGACA 
GGAAGTATTG GAGAGAGAAC TGGAATCTTC CCGTCCAACT ACGTCAGACC AAAGGATCAA 
GAGAATTTTG GGAATGCTAG CAAATCTGGA GCATCAAACA AAAAACCCGA GATCGCTCAA 
GTAACTTCAG CATATGCTGC TTCAGGGACT GAGCAGCTCA GCCTTGCGCC AGGACAGTTA 
ATATTAATCT TAAAGAAAAA CACAAGCGGG TGGTGGCAAG GAGAGCTACA GGCCAGAGGG 
AAGAAACGAC AGAAGGGATG GTTTCCTGCC AGCCATGTAA AGCTGCTAGG TCCAAGCAGT 
GAAAGAACCA TGCCTACTTT TCACGCTGTA TGTCAAGTGA TTGCTATGTA TGACTACATG 
GCGAATAACG AAGATGAGCT CAATTTCTCC AAAGGACAGC TGATTAATGT TATGAACAAA 
GATGACCCTG ACTGGTGGCA AGGAGAAACC AATGGTCTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCAGATCCA AGTCAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515090.1
CDS	227..3688
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQ

Target ORF information:

RefSeq Version	XM_006515027.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515027.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGGAAC TCAGAGAAAG CTATAATACA CAGCAGTTAG CCCTTGAACA ACTTCATAAA 
ATCAAACGTG ACAAATTGAA GGAAATCGAA AGAAAAAGAT TAGAGCAAAT TCAAAAAAAG 
AAACTAGAAG ATGAGGCTGC AAGGAAAGCA AAGCAAGGAA AAGAAAACTT GTGGAGAGAA 
AGTATTAGAA AGGAAGAAGA GGAAAAGCAA AAACGACTCC AGGAAGAAAA GTCACAGGAC 
AAAACTCAAG AAGAGGAACG AAAAGCTGAG GCAAAACAAA GTGAGACAGC CAGTGCTTTG 
GTGAATTACA GAGCACTGTA CCCTTTTGAA GCAAGAAACC ATGATGAGAT GAGTTTTAGT 
TCTGGGGATA TAATTCAGGT TGATGAAAAA ACTGTAGGAG AGCCTGGTTG GCTTTATGGT 
AGTTTTCAGG GAAAGTTTGG CTGGTTCCCG TGCAACTATG TAGAAAAAGT GCTGTCAAGT 
GAAAAAGCTC TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTGTCTCT CTCTGCTACC 
TCAACTTCTT CCCAGCCACC AGCATCAGTG ACTGATTATC ACAATGTATC CTTCTCAAAC 
CTTACTGTTA ATACAACATG GCAGCAGAAG TCAGCTTTTA CCCGCACTGT GTCCCCTGGA 
TCTGTGTCCC CCATTCACGG ACAGGGGCAG GCTGTAGAAA ACCTGAAAGC CCAGGCCCTT 
TGTTCCTGGA CGGCAAAGAA GGAGAACCAC CTGAACTTCT CAAAGCACGA CGTCATCACT 
GTCCTGGAGC AGCAGGAAAA CTGGTGGTTT GGGGAGGTGC ACGGAGGAAG AGGATGGTTC 
CCCAAGTCTT ATGTCAAGCT CATTCCTGGG AATGAAGTAC AGCGAGGAGA GCCAGAAGCT 
TTGTATGCAG CTGTGACTAA GAAACCTACC TCCACAGCCT ATCCAGTTAC CTCCACAGCC 
TATCCAGTTG GAGAAGACTA CATTGCACTT TATTCATACT CAAGTGTAGA GCCCGGGGAT 
TTGACTTTCA CTGAAGGTGA AGAAATTCTA GTGACCCAGA AAGATGGAGA GTGGTGGACA 
GGAAGTATTG GAGAGAGAAC TGGAATCTTC CCGTCCAACT ACGTCAGACC AAAGGATCAA 
GAGAATTTTG GGAATGCTAG CAAATCTGGA GCATCAAACA AAAAACCCGA GATCGCTCAA 
GTAACTTCAG CATATGCTGC TTCAGGGACT GAGCAGCTCA GCCTTGCGCC AGGACAGTTA 
ATATTAATCT TAAAGAAAAA CACAAGCGGG TGGTGGCAAG GAGAGCTACA GGCCAGAGGG 
AAGAAACGAC AGAAGGGATG GTTTCCTGCC AGCCATGTAA AGCTGCTAGG TCCAAGCAGT 
GAAAGAACCA TGCCTACTTT TCACGCTGTA TGTCAAGTGA TTGCTATGTA TGACTACATG 
GCGAATAACG AAGATGAGCT CAATTTCTCC AAAGGACAGC TGATTAATGT TATGAACAAA 
GATGACCCTG ACTGGTGGCA AGGAGAAACC AATGGTCTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCAGATCCA AGTCAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_030246627.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102487.1 Mus musculus intersectin 2 (Itsn2), transcript variant X1, mRNA.

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CloneID	OMu01935
Clone ID Related Accession (Same CDS sequence)	NM_001198968.2 , XM_006515024.3 , XM_030246627.1 , XM_006515023.3 , XM_006515023.4 , XM_006515024.4
Accession Version	XM_030246627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102487.1
CDS	293..5350
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_030246627.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246627.1

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515023.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515086.1 Mus musculus intersectin 2 (Itsn2), transcript variant X2, mRNA.

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CloneID	OMu01935
Clone ID Related Accession (Same CDS sequence)	NM_001198968.2 , XM_006515024.3 , XM_030246627.1 , XM_006515023.3 , XM_006515023.4 , XM_006515024.4
Accession Version	XM_006515023.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515023.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515086.1
CDS	141..5198
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_006515023.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515023.4

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515024.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515087.1 Mus musculus intersectin 2 (Itsn2), transcript variant X3, mRNA.

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CloneID	OMu01935
Clone ID Related Accession (Same CDS sequence)	NM_001198968.2 , XM_006515024.3 , XM_030246627.1 , XM_006515023.3 , XM_006515023.4 , XM_006515024.4
Accession Version	XM_006515024.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515024.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515087.1
CDS	322..5379
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_006515024.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515024.4

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515025.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515088.1 Mus musculus intersectin 2 (Itsn2), transcript variant X4, mRNA.

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CloneID	OMu47268
Clone ID Related Accession (Same CDS sequence)	XM_006515025.4 , XM_006515025.3
Accession Version	XM_006515025.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACATCTGA AGAACGTACT AAGCATGATA AACAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCCCGTACTT TTTTCCTACA GTCAGGTCTG 
CCGGCCCCGG TTTTAGCTGA AATATGGGCC TTATCAGATC TGAACAAGGA TGGGAAGATG 
GACCAGCAAG AGTTCTCTAT AGCTATGAAA CTCATCAAGT TAAAGTTGCA GGGCCAACAG 
CTGCCTGTAG TCCTCCCTCC TATCATGAAA CAACCCCCTA TGTTCTCTCC ACTAATCTCT 
GCTCGTTTTG GGATGGGAAG CATGCCCAAT CTGTCCATTC ATCAGCCATT GCCTCCAGTT 
GCACCTATAG CAACACCCTT GTCTTCTGCT ACTTCAGGGA CCAGTATTCC TCCCCTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGT ACATCCTCAT TACCAAATGG AACTGCCAGT 
CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATGC ATCATCTTAC 
AGCCTGATGA TGGGAGGATT TGGTGGTGCT AGTATCCAGA AGGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAACTC ACCTAAGACA 
GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AGTATCGGCA AAAATTTAAT 
AGTCTAGACA AAGGCATGAG CGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TCTCTCAAAC TCAGCTAGCT ACTATTTGGA CTCTGGCTGA CATCGATGGT 
GACGGACAGT TGAAAGCTGA AGAATTTATT CTGGCGATGC ACCTCACTGA CATGGCCAAA 
GCTGGACAGC CACTACCACT GACGTTGCCT CCCGAGCTTG TCCCTCCATC TTTCAGAGGG 
GGAAAGCAAG TTGATTCTGT TAATGGAACT CTGCCTTCAT ATCAGAAAAC ACAAGAAGAA 
GAGCCTCAGA AGAAACTGCC AGTTACTTTT GAGGACAAAC GGAAAGCCAA CTATGAACGA 
GGAAACATGG AGCTGGAGAA GCGACGCCAA GTGTTGATGG AGCAGCAGCA GAGGGAGGCT 
GAACGCAAAG CCCAGAAAGA GAAGGAAGAG TGGGAGCGGA AACAGAGAGA ACTGCAAGAG 
CAAGAATGGA AGAAGCAGCT GGAGTTGGAG AAACGCTTGG AGAAACAGAG AGAGCTGGAG 
AGACAGCGGG AGGAAGAGAG GAGAAAGGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAG 
CTTGAGAGAC AACGCCGTTT AGAATGGGAA AGACTCCGTC GGCAGGAGCT GCTCAGTCAG 
AAGACCAGGG AACAAGAAGA CATTGTCAGG CTGAGCTCCA GAAAGAAAAG TCTCCACCTG 
GAACTGGAAG CAGTGAATGG AAAACATCAG CAGATCTCAG GCAGACTACA AGATGTCCAA 
ATCAGAAAGC AAACACAAAA GACTGAGCTA GAAGTTTTGG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAACAACT TCAACAAGAG CTTAAGGAAT ATCAAAATAA GCTTATCTAT 
CTGGTCCCTG AGAAGCAGCT ATTAAACGAA AGAATTAAAA ACATGCAGCT CAGTAACACA 
CCTGATTCAG GGATCAGTTT ACTTCATAAA AAGTCATCAG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT CTCAGAAATG 
GATTCATTTA ACAATCAGCT GAAGTGTGGG AGTATGGATG ACTGTGTCCT TCAGTGCCTT 
TTGTCTCTGC TGAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT CAGAGAAAGC 
TATAATACAC AGCAGTTAGC CCTTGAACAA CTTCATAAAA TCAAACGTGA CAAATTGAAG 
GAAATCGAAA GAAAAAGATT AGAGCAAATT CAAAAAAAGA AACTAGAAGA TGAGGCTGCA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTG TGGAGAGAAA GTATTAGAAA GGAAGAAGAG 
GAAAAGCAAA AACGACTCCA GGAAGAAAAG TCACAGGACA AAACTCAAGA AGAGGAACGA 
AAAGCTGAGG CAAAACAAAG TGAGACAGCC AGTGCTTTGG TGAATTACAG AGCACTGTAC 
CCTTTTGAAG CAAGAAACCA TGATGAGATG AGTTTTAGTT CTGGGGATAT AATTCAGGTT 
GATGAAAAAA CTGTAGGAGA GCCTGGTTGG CTTTATGGTA GTTTTCAGGG AAAGTTTGGC 
TGGTTCCCGT GCAACTATGT AGAAAAAGTG CTGTCAAGTG AAAAAGCTCT GTCTCCTAAG 
AAGGCCTTAC TTCCTCCTAC AGTGTCTCTC TCTGCTACCT CAACTTCTTC CCAGCCACCA 
GCATCAGTGA CTGATTATCA CAATGTATCC TTCTCAAACC TTACTGTTAA TACAACATGG 
CAGCAGAAGT CAGCTTTTAC CCGCACTGTG TCCCCTGGAT CTGTGTCCCC CATTCACGGA 
CAGGGGCAGG CTGTAGAAAA CCTGAAAGCC CAGGCCCTTT GTTCCTGGAC GGCAAAGAAG 
GAGAACCACC TGAACTTCTC AAAGCACGAC GTCATCACTG TCCTGGAGCA GCAGGAAAAC 
TGGTGGTTTG GGGAGGTGCA CGGAGGAAGA GGATGGTTCC CCAAGTCTTA TGTCAAGCTC 
ATTCCTGGGA ATGAAGTACA GCGAGGAGAG CCAGAAGCTT TGTATGCAGC TGTGACTAAG 
AAACCTACCT CCACAGCCTA TCCAGTTACC TCCACAGCCT ATCCAGTTGG AGAAGACTAC 
ATTGCACTTT ATTCATACTC AAGTGTAGAG CCCGGGGATT TGACTTTCAC TGAAGGTGAA 
GAAATTCTAG TGACCCAGAA AGATGGAGAG TGGTGGACAG GAAGTATTGG AGAGAGAACT 
GGAATCTTCC CGTCCAACTA CGTCAGACCA AAGGATCAAG AGAATTTTGG GAATGCTAGC 
AAATCTGGAG CATCAAACAA AAAACCCGAG ATCGCTCAAG TAACTTCAGC ATATGCTGCT 
TCAGGGACTG AGCAGCTCAG CCTTGCGCCA GGACAGTTAA TATTAATCTT AAAGAAAAAC 
ACAAGCGGGT GGTGGCAAGG AGAGCTACAG GCCAGAGGGA AGAAACGACA GAAGGGATGG 
TTTCCTGCCA GCCATGTAAA GCTGCTAGGT CCAAGCAGTG AAAGAACCAT GCCTACTTTT 
CACGCTGTAT GTCAAGTGAT TGCTATGTAT GACTACATGG CGAATAACGA AGATGAGCTC 
AATTTCTCCA AAGGACAGCT GATTAATGTT ATGAACAAAG ATGACCCTGA CTGGTGGCAA 
GGAGAAACCA ATGGTCTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCAGATCCAA GTCAACAGTG GTGTGCTGAC CTCCAAGCCC TGGACACAAT GCAGCCTACG 
GAGAGGAAGC GACAGGGCTA CATTCACGAG CTCATTCAGA CAGAGGAGCG GTACATGGAC 
GACCTGCAGC TGGTCATCGA GGTCTTCCAG AAACGGATGG CTGAGTCAGG CTTCCTCACT 
GAAGCAGACA TGGCTCTGAT CTTTGTGAAC TGGAAAGAGC TCATCATGTC CAACACGAAG 
CTGCTGAGGG CCTTGCGGGT GAGGAAGAAG ACTGGGGGTG AGAAGATGCC AGTTCAGATG 
ATTGGAGACA TCCTGGCGGC AGAGCTGTCC CACATGCAGG CCTACATCCG CTTCTGCAGC 
TGTCAGCTTA ATGGGGCAAC CCTGTTACAG CAGAAGACAG ACGAGGACAC GGACTTCAAG 
GAATTTCTAA AGAAGTTGGC ATCAGACCCA CGATGCAAAG GGATGCCCCT CTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG GATCACTCGC TACCCGCTGC TCATCCGAAG TATCCTGGAG 
AACACTCCAC AGAGTCATGT TGACCACTCC TCCCTGAAGC TGGCCCTAGA ACGTGCTGAG 
GAGCTGTGCT CTCAGGTGAA CGAGGGAGTC CGGGAGAAGG AAAATTCAGA CCGGCTGGAG 
TGGATCCAGG CACACGTGCA GTGCGAAGGC TTGGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGCCCCCG GAAGCTTCTG CACAGCGGGA AGCTGTACAA GACCAAGAGC 
AATAAGGAGC TGCACGCCTT CCTCTTCAAC GACTTCCTGC TGCTCACCTA CCTGGTCAGG 
CAGTTTGCCG CCGCCTCTGG CCACGAGAAG CTCTTCAACT CCAAGTCCAG TGCTCAGTTC 
CGGATGTACA AAACGCCCAT TTTCCTGAAT GAAGTGTTGG TGAAACTTCC CACAGACCCT 
TCCAGCGATG AGCCCGTCTT CCACATTTCC CACATTGATC GTGTGTACAC ACTCCGAACA 
GACAACATCA ACGAGAGGAC GGCCTGGGTC CAGAAGATCA AGGGTGCCTC AGAGCAGTAC 
ATCGACACTG AGAAGAAGAA ACGGGAAAAG GCTTACCAAG CCCGTTCTCA AAAGACTTCA 
GGTATTGGGC GTCTGATGGT GCATGTCATT GAAGCTACAG AATTAAAAGC CTGCAAACCA 
AACGGGAAAA GTAATCCATA CTGTGAAGTC AGCATGGGCT CCCAAAGCTA TACCACCAGG 
ACCCTGCAGG ACACACTAAA CCCCAAGTGG AACTTCAACT GCCAGTTCTT CATCAAGGAT 
CTTTACCAGG ACGTTCTGTG TCTCACTATG TTTGACAGAG ACCAGTTTTC TCCAGATGAC 
TTCTTGGGTC GTACTGAAGT TCCAGTGGCA AAAATCCGAA CAGAACAGGA AAGCAAAGGC 
CCCACCACCC GCCGACTACT ACTGCACGAA GTCCCCACTG GAGAAGTCTG GGTCCGCTTT 
GACCTGCAAC TTTTTGAACA AAAAACTCTC CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515088.1
CDS	88..5103
Translation	MWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_006515025.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515025.4

ORF Insert Sequence:

ATGTGGGCTA TTACATCTGA AGAACGTACT AAGCATGATA AACAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCCCGTACTT TTTTCCTACA GTCAGGTCTG 
CCGGCCCCGG TTTTAGCTGA AATATGGGCC TTATCAGATC TGAACAAGGA TGGGAAGATG 
GACCAGCAAG AGTTCTCTAT AGCTATGAAA CTCATCAAGT TAAAGTTGCA GGGCCAACAG 
CTGCCTGTAG TCCTCCCTCC TATCATGAAA CAACCCCCTA TGTTCTCTCC ACTAATCTCT 
GCTCGTTTTG GGATGGGAAG CATGCCCAAT CTGTCCATTC ATCAGCCATT GCCTCCAGTT 
GCACCTATAG CAACACCCTT GTCTTCTGCT ACTTCAGGGA CCAGTATTCC TCCCCTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGT ACATCCTCAT TACCAAATGG AACTGCCAGT 
CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATGC ATCATCTTAC 
AGCCTGATGA TGGGAGGATT TGGTGGTGCT AGTATCCAGA AGGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAACTC ACCTAAGACA 
GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AGTATCGGCA AAAATTTAAT 
AGTCTAGACA AAGGCATGAG CGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TCTCTCAAAC TCAGCTAGCT ACTATTTGGA CTCTGGCTGA CATCGATGGT 
GACGGACAGT TGAAAGCTGA AGAATTTATT CTGGCGATGC ACCTCACTGA CATGGCCAAA 
GCTGGACAGC CACTACCACT GACGTTGCCT CCCGAGCTTG TCCCTCCATC TTTCAGAGGG 
GGAAAGCAAG TTGATTCTGT TAATGGAACT CTGCCTTCAT ATCAGAAAAC ACAAGAAGAA 
GAGCCTCAGA AGAAACTGCC AGTTACTTTT GAGGACAAAC GGAAAGCCAA CTATGAACGA 
GGAAACATGG AGCTGGAGAA GCGACGCCAA GTGTTGATGG AGCAGCAGCA GAGGGAGGCT 
GAACGCAAAG CCCAGAAAGA GAAGGAAGAG TGGGAGCGGA AACAGAGAGA ACTGCAAGAG 
CAAGAATGGA AGAAGCAGCT GGAGTTGGAG AAACGCTTGG AGAAACAGAG AGAGCTGGAG 
AGACAGCGGG AGGAAGAGAG GAGAAAGGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAG 
CTTGAGAGAC AACGCCGTTT AGAATGGGAA AGACTCCGTC GGCAGGAGCT GCTCAGTCAG 
AAGACCAGGG AACAAGAAGA CATTGTCAGG CTGAGCTCCA GAAAGAAAAG TCTCCACCTG 
GAACTGGAAG CAGTGAATGG AAAACATCAG CAGATCTCAG GCAGACTACA AGATGTCCAA 
ATCAGAAAGC AAACACAAAA GACTGAGCTA GAAGTTTTGG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAACAACT TCAACAAGAG CTTAAGGAAT ATCAAAATAA GCTTATCTAT 
CTGGTCCCTG AGAAGCAGCT ATTAAACGAA AGAATTAAAA ACATGCAGCT CAGTAACACA 
CCTGATTCAG GGATCAGTTT ACTTCATAAA AAGTCATCAG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT CTCAGAAATG 
GATTCATTTA ACAATCAGCT GAAGTGTGGG AGTATGGATG ACTGTGTCCT TCAGTGCCTT 
TTGTCTCTGC TGAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT CAGAGAAAGC 
TATAATACAC AGCAGTTAGC CCTTGAACAA CTTCATAAAA TCAAACGTGA CAAATTGAAG 
GAAATCGAAA GAAAAAGATT AGAGCAAATT CAAAAAAAGA AACTAGAAGA TGAGGCTGCA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTG TGGAGAGAAA GTATTAGAAA GGAAGAAGAG 
GAAAAGCAAA AACGACTCCA GGAAGAAAAG TCACAGGACA AAACTCAAGA AGAGGAACGA 
AAAGCTGAGG CAAAACAAAG TGAGACAGCC AGTGCTTTGG TGAATTACAG AGCACTGTAC 
CCTTTTGAAG CAAGAAACCA TGATGAGATG AGTTTTAGTT CTGGGGATAT AATTCAGGTT 
GATGAAAAAA CTGTAGGAGA GCCTGGTTGG CTTTATGGTA GTTTTCAGGG AAAGTTTGGC 
TGGTTCCCGT GCAACTATGT AGAAAAAGTG CTGTCAAGTG AAAAAGCTCT GTCTCCTAAG 
AAGGCCTTAC TTCCTCCTAC AGTGTCTCTC TCTGCTACCT CAACTTCTTC CCAGCCACCA 
GCATCAGTGA CTGATTATCA CAATGTATCC TTCTCAAACC TTACTGTTAA TACAACATGG 
CAGCAGAAGT CAGCTTTTAC CCGCACTGTG TCCCCTGGAT CTGTGTCCCC CATTCACGGA 
CAGGGGCAGG CTGTAGAAAA CCTGAAAGCC CAGGCCCTTT GTTCCTGGAC GGCAAAGAAG 
GAGAACCACC TGAACTTCTC AAAGCACGAC GTCATCACTG TCCTGGAGCA GCAGGAAAAC 
TGGTGGTTTG GGGAGGTGCA CGGAGGAAGA GGATGGTTCC CCAAGTCTTA TGTCAAGCTC 
ATTCCTGGGA ATGAAGTACA GCGAGGAGAG CCAGAAGCTT TGTATGCAGC TGTGACTAAG 
AAACCTACCT CCACAGCCTA TCCAGTTACC TCCACAGCCT ATCCAGTTGG AGAAGACTAC 
ATTGCACTTT ATTCATACTC AAGTGTAGAG CCCGGGGATT TGACTTTCAC TGAAGGTGAA 
GAAATTCTAG TGACCCAGAA AGATGGAGAG TGGTGGACAG GAAGTATTGG AGAGAGAACT 
GGAATCTTCC CGTCCAACTA CGTCAGACCA AAGGATCAAG AGAATTTTGG GAATGCTAGC 
AAATCTGGAG CATCAAACAA AAAACCCGAG ATCGCTCAAG TAACTTCAGC ATATGCTGCT 
TCAGGGACTG AGCAGCTCAG CCTTGCGCCA GGACAGTTAA TATTAATCTT AAAGAAAAAC 
ACAAGCGGGT GGTGGCAAGG AGAGCTACAG GCCAGAGGGA AGAAACGACA GAAGGGATGG 
TTTCCTGCCA GCCATGTAAA GCTGCTAGGT CCAAGCAGTG AAAGAACCAT GCCTACTTTT 
CACGCTGTAT GTCAAGTGAT TGCTATGTAT GACTACATGG CGAATAACGA AGATGAGCTC 
AATTTCTCCA AAGGACAGCT GATTAATGTT ATGAACAAAG ATGACCCTGA CTGGTGGCAA 
GGAGAAACCA ATGGTCTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCAGATCCAA GTCAACAGTG GTGTGCTGAC CTCCAAGCCC TGGACACAAT GCAGCCTACG 
GAGAGGAAGC GACAGGGCTA CATTCACGAG CTCATTCAGA CAGAGGAGCG GTACATGGAC 
GACCTGCAGC TGGTCATCGA GGTCTTCCAG AAACGGATGG CTGAGTCAGG CTTCCTCACT 
GAAGCAGACA TGGCTCTGAT CTTTGTGAAC TGGAAAGAGC TCATCATGTC CAACACGAAG 
CTGCTGAGGG CCTTGCGGGT GAGGAAGAAG ACTGGGGGTG AGAAGATGCC AGTTCAGATG 
ATTGGAGACA TCCTGGCGGC AGAGCTGTCC CACATGCAGG CCTACATCCG CTTCTGCAGC 
TGTCAGCTTA ATGGGGCAAC CCTGTTACAG CAGAAGACAG ACGAGGACAC GGACTTCAAG 
GAATTTCTAA AGAAGTTGGC ATCAGACCCA CGATGCAAAG GGATGCCCCT CTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG GATCACTCGC TACCCGCTGC TCATCCGAAG TATCCTGGAG 
AACACTCCAC AGAGTCATGT TGACCACTCC TCCCTGAAGC TGGCCCTAGA ACGTGCTGAG 
GAGCTGTGCT CTCAGGTGAA CGAGGGAGTC CGGGAGAAGG AAAATTCAGA CCGGCTGGAG 
TGGATCCAGG CACACGTGCA GTGCGAAGGC TTGGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGCCCCCG GAAGCTTCTG CACAGCGGGA AGCTGTACAA GACCAAGAGC 
AATAAGGAGC TGCACGCCTT CCTCTTCAAC GACTTCCTGC TGCTCACCTA CCTGGTCAGG 
CAGTTTGCCG CCGCCTCTGG CCACGAGAAG CTCTTCAACT CCAAGTCCAG TGCTCAGTTC 
CGGATGTACA AAACGCCCAT TTTCCTGAAT GAAGTGTTGG TGAAACTTCC CACAGACCCT 
TCCAGCGATG AGCCCGTCTT CCACATTTCC CACATTGATC GTGTGTACAC ACTCCGAACA 
GACAACATCA ACGAGAGGAC GGCCTGGGTC CAGAAGATCA AGGGTGCCTC AGAGCAGTAC 
ATCGACACTG AGAAGAAGAA ACGGGAAAAG GCTTACCAAG CCCGTTCTCA AAAGACTTCA 
GGTATTGGGC GTCTGATGGT GCATGTCATT GAAGCTACAG AATTAAAAGC CTGCAAACCA 
AACGGGAAAA GTAATCCATA CTGTGAAGTC AGCATGGGCT CCCAAAGCTA TACCACCAGG 
ACCCTGCAGG ACACACTAAA CCCCAAGTGG AACTTCAACT GCCAGTTCTT CATCAAGGAT 
CTTTACCAGG ACGTTCTGTG TCTCACTATG TTTGACAGAG ACCAGTTTTC TCCAGATGAC 
TTCTTGGGTC GTACTGAAGT TCCAGTGGCA AAAATCCGAA CAGAACAGGA AAGCAAAGGC 
CCCACCACCC GCCGACTACT ACTGCACGAA GTCCCCACTG GAGAAGTCTG GGTCCGCTTT 
GACCTGCAAC TTTTTGAACA AAAAACTCTC CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( NM_001198968.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185897.1 Mus musculus intersectin 2 (Itsn2), transcript variant 1, mRNA.

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CloneID	OMu01935
Clone ID Related Accession (Same CDS sequence)	NM_001198968.2 , XM_006515024.3 , XM_030246627.1 , XM_006515023.3 , XM_006515023.4 , XM_006515024.4
Accession Version	NM_001198968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC241534.2, BC138262.1 and BC037119.1. On Mar 7, 2012 this sequence version replaced NM_001198968.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185897.1
CDS	258..5315
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_001198968.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198968.2

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( NM_011365.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035495.2 Mus musculus intersectin 2 (Itsn2), transcript variant 2, mRNA.

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CloneID	OMu08005
Clone ID Related Accession (Same CDS sequence)	NM_011365.4
Accession Version	NM_011365.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC241534.2, BC138262.1 and BC037119.1. On Mar 23, 2012 this sequence version replaced NM_011365.3. Transcript Variant: This variant (2) lacks an in-frame alternate exon in the 5' coding region, compared to variant 1, which results in a shorter isoform (2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGGAAC TCAGAGAAAG CTATAATACA CAGCAGTTAG CCCTTGAACA ACTTCATAAA 
ATCAAACGTG ACAAATTGAA GGAAATCGAA AGAAAAAGAT TAGAGCAAAT TCAAAAAAAG 
AAACTAGAAG ATGAGGCTGC AAGGAAAGCA AAGCAAGGAA AAGAAAACTT GTGGAGAGAA 
AGTATTAGAA AGGAAGAAGA GGAAAAGCAA AAACGACTCC AGGAAGAAAA GTCACAGGAC 
AAAACTCAAG AAGAGGAACG AAAAGCTGAG GCAAAACAAA GTGAGACAGC CAGTGCTTTG 
GTGAATTACA GAGCACTGTA CCCTTTTGAA GCAAGAAACC ATGATGAGAT GAGTTTTAGT 
TCTGGGGATA TAATTCAGGT TGATGAAAAA ACTGTAGGAG AGCCTGGTTG GCTTTATGGT 
AGTTTTCAGG GAAAGTTTGG CTGGTTCCCG TGCAACTATG TAGAAAAAGT GCTGTCAAGT 
GAAAAAGCTC TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTGTCTCT CTCTGCTACC 
TCAACTTCTT CCCAGCCACC AGCATCAGTG ACTGATTATC ACAATGTATC CTTCTCAAAC 
CTTACTGTTA ATACAACATG GCAGCAGAAG TCAGCTTTTA CCCGCACTGT GTCCCCTGGA 
TCTGTGTCCC CCATTCACGG ACAGGGGCAG GCTGTAGAAA ACCTGAAAGC CCAGGCCCTT 
TGTTCCTGGA CGGCAAAGAA GGAGAACCAC CTGAACTTCT CAAAGCACGA CGTCATCACT 
GTCCTGGAGC AGCAGGAAAA CTGGTGGTTT GGGGAGGTGC ACGGAGGAAG AGGATGGTTC 
CCCAAGTCTT ATGTCAAGCT CATTCCTGGG AATGAAGTAC AGCGAGGAGA GCCAGAAGCT 
TTGTATGCAG CTGTGACTAA GAAACCTACC TCCACAGCCT ATCCAGTTAC CTCCACAGCC 
TATCCAGTTG GAGAAGACTA CATTGCACTT TATTCATACT CAAGTGTAGA GCCCGGGGAT 
TTGACTTTCA CTGAAGGTGA AGAAATTCTA GTGACCCAGA AAGATGGAGA GTGGTGGACA 
GGAAGTATTG GAGAGAGAAC TGGAATCTTC CCGTCCAACT ACGTCAGACC AAAGGATCAA 
GAGAATTTTG GGAATGCTAG CAAATCTGGA GCATCAAACA AAAAACCCGA GATCGCTCAA 
GTAACTTCAG CATATGCTGC TTCAGGGACT GAGCAGCTCA GCCTTGCGCC AGGACAGTTA 
ATATTAATCT TAAAGAAAAA CACAAGCGGG TGGTGGCAAG GAGAGCTACA GGCCAGAGGG 
AAGAAACGAC AGAAGGGATG GTTTCCTGCC AGCCATGTAA AGCTGCTAGG TCCAAGCAGT 
GAAAGAACCA TGCCTACTTT TCACGCTGTA TGTCAAGTGA TTGCTATGTA TGACTACATG 
GCGAATAACG AAGATGAGCT CAATTTCTCC AAAGGACAGC TGATTAATGT TATGAACAAA 
GATGACCCTG ACTGGTGGCA AGGAGAAACC AATGGTCTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCAGATCCA AGTCAACAGT GGTGTGCTGA CCTCCAAGCC 
CTGGACACAA TGCAGCCTAC GGAGAGGAAG CGACAGGGCT ACATTCACGA GCTCATTCAG 
ACAGAGGAGC GGTACATGGA CGACCTGCAG CTGGTCATCG AGGTCTTCCA GAAACGGATG 
GCTGAGTCAG GCTTCCTCAC TGAAGCAGAC ATGGCTCTGA TCTTTGTGAA CTGGAAAGAG 
CTCATCATGT CCAACACGAA GCTGCTGAGG GCCTTGCGGG TGAGGAAGAA GACTGGGGGT 
GAGAAGATGC CAGTTCAGAT GATTGGAGAC ATCCTGGCGG CAGAGCTGTC CCACATGCAG 
GCCTACATCC GCTTCTGCAG CTGTCAGCTT AATGGGGCAA CCCTGTTACA GCAGAAGACA 
GACGAGGACA CGGACTTCAA GGAATTTCTA AAGAAGTTGG CATCAGACCC ACGATGCAAA 
GGGATGCCCC TCTCCAGCTT CCTGCTGAAG CCCATGCAGA GGATCACTCG CTACCCGCTG 
CTCATCCGAA GTATCCTGGA GAACACTCCA CAGAGTCATG TTGACCACTC CTCCCTGAAG 
CTGGCCCTAG AACGTGCTGA GGAGCTGTGC TCTCAGGTGA ACGAGGGAGT CCGGGAGAAG 
GAAAATTCAG ACCGGCTGGA GTGGATCCAG GCACACGTGC AGTGCGAAGG CTTGGCAGAG 
CAACTTATTT TCAACTCCCT CACCAACTGC CTGGGCCCCC GGAAGCTTCT GCACAGCGGG 
AAGCTGTACA AGACCAAGAG CAATAAGGAG CTGCACGCCT TCCTCTTCAA CGACTTCCTG 
CTGCTCACCT ACCTGGTCAG GCAGTTTGCC GCCGCCTCTG GCCACGAGAA GCTCTTCAAC 
TCCAAGTCCA GTGCTCAGTT CCGGATGTAC AAAACGCCCA TTTTCCTGAA TGAAGTGTTG 
GTGAAACTTC CCACAGACCC TTCCAGCGAT GAGCCCGTCT TCCACATTTC CCACATTGAT 
CGTGTGTACA CACTCCGAAC AGACAACATC AACGAGAGGA CGGCCTGGGT CCAGAAGATC 
AAGGGTGCCT CAGAGCAGTA CATCGACACT GAGAAGAAGA AACGGGAAAA GGCTTACCAA 
GCCCGTTCTC AAAAGACTTC AGGTATTGGG CGTCTGATGG TGCATGTCAT TGAAGCTACA 
GAATTAAAAG CCTGCAAACC AAACGGGAAA AGTAATCCAT ACTGTGAAGT CAGCATGGGC 
TCCCAAAGCT ATACCACCAG GACCCTGCAG GACACACTAA ACCCCAAGTG GAACTTCAAC 
TGCCAGTTCT TCATCAAGGA TCTTTACCAG GACGTTCTGT GTCTCACTAT GTTTGACAGA 
GACCAGTTTT CTCCAGATGA CTTCTTGGGT CGTACTGAAG TTCCAGTGGC AAAAATCCGA 
ACAGAACAGG AAAGCAAAGG CCCCACCACC CGCCGACTAC TACTGCACGA AGTCCCCACT 
GGAGAAGTCT GGGTCCGCTT TGACCTGCAA CTTTTTGAAC AAAAAACTCT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035495.2
CDS	258..5234
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	NM_011365.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011365.4

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGGAAC TCAGAGAAAG CTATAATACA CAGCAGTTAG CCCTTGAACA ACTTCATAAA 
ATCAAACGTG ACAAATTGAA GGAAATCGAA AGAAAAAGAT TAGAGCAAAT TCAAAAAAAG 
AAACTAGAAG ATGAGGCTGC AAGGAAAGCA AAGCAAGGAA AAGAAAACTT GTGGAGAGAA 
AGTATTAGAA AGGAAGAAGA GGAAAAGCAA AAACGACTCC AGGAAGAAAA GTCACAGGAC 
AAAACTCAAG AAGAGGAACG AAAAGCTGAG GCAAAACAAA GTGAGACAGC CAGTGCTTTG 
GTGAATTACA GAGCACTGTA CCCTTTTGAA GCAAGAAACC ATGATGAGAT GAGTTTTAGT 
TCTGGGGATA TAATTCAGGT TGATGAAAAA ACTGTAGGAG AGCCTGGTTG GCTTTATGGT 
AGTTTTCAGG GAAAGTTTGG CTGGTTCCCG TGCAACTATG TAGAAAAAGT GCTGTCAAGT 
GAAAAAGCTC TGTCTCCTAA GAAGGCCTTA CTTCCTCCTA CAGTGTCTCT CTCTGCTACC 
TCAACTTCTT CCCAGCCACC AGCATCAGTG ACTGATTATC ACAATGTATC CTTCTCAAAC 
CTTACTGTTA ATACAACATG GCAGCAGAAG TCAGCTTTTA CCCGCACTGT GTCCCCTGGA 
TCTGTGTCCC CCATTCACGG ACAGGGGCAG GCTGTAGAAA ACCTGAAAGC CCAGGCCCTT 
TGTTCCTGGA CGGCAAAGAA GGAGAACCAC CTGAACTTCT CAAAGCACGA CGTCATCACT 
GTCCTGGAGC AGCAGGAAAA CTGGTGGTTT GGGGAGGTGC ACGGAGGAAG AGGATGGTTC 
CCCAAGTCTT ATGTCAAGCT CATTCCTGGG AATGAAGTAC AGCGAGGAGA GCCAGAAGCT 
TTGTATGCAG CTGTGACTAA GAAACCTACC TCCACAGCCT ATCCAGTTAC CTCCACAGCC 
TATCCAGTTG GAGAAGACTA CATTGCACTT TATTCATACT CAAGTGTAGA GCCCGGGGAT 
TTGACTTTCA CTGAAGGTGA AGAAATTCTA GTGACCCAGA AAGATGGAGA GTGGTGGACA 
GGAAGTATTG GAGAGAGAAC TGGAATCTTC CCGTCCAACT ACGTCAGACC AAAGGATCAA 
GAGAATTTTG GGAATGCTAG CAAATCTGGA GCATCAAACA AAAAACCCGA GATCGCTCAA 
GTAACTTCAG CATATGCTGC TTCAGGGACT GAGCAGCTCA GCCTTGCGCC AGGACAGTTA 
ATATTAATCT TAAAGAAAAA CACAAGCGGG TGGTGGCAAG GAGAGCTACA GGCCAGAGGG 
AAGAAACGAC AGAAGGGATG GTTTCCTGCC AGCCATGTAA AGCTGCTAGG TCCAAGCAGT 
GAAAGAACCA TGCCTACTTT TCACGCTGTA TGTCAAGTGA TTGCTATGTA TGACTACATG 
GCGAATAACG AAGATGAGCT CAATTTCTCC AAAGGACAGC TGATTAATGT TATGAACAAA 
GATGACCCTG ACTGGTGGCA AGGAGAAACC AATGGTCTGA CTGGTCTCTT TCCTTCAAAC 
TATGTTAAGA TGACAACAGA CTCAGATCCA AGTCAACAGT GGTGTGCTGA CCTCCAAGCC 
CTGGACACAA TGCAGCCTAC GGAGAGGAAG CGACAGGGCT ACATTCACGA GCTCATTCAG 
ACAGAGGAGC GGTACATGGA CGACCTGCAG CTGGTCATCG AGGTCTTCCA GAAACGGATG 
GCTGAGTCAG GCTTCCTCAC TGAAGCAGAC ATGGCTCTGA TCTTTGTGAA CTGGAAAGAG 
CTCATCATGT CCAACACGAA GCTGCTGAGG GCCTTGCGGG TGAGGAAGAA GACTGGGGGT 
GAGAAGATGC CAGTTCAGAT GATTGGAGAC ATCCTGGCGG CAGAGCTGTC CCACATGCAG 
GCCTACATCC GCTTCTGCAG CTGTCAGCTT AATGGGGCAA CCCTGTTACA GCAGAAGACA 
GACGAGGACA CGGACTTCAA GGAATTTCTA AAGAAGTTGG CATCAGACCC ACGATGCAAA 
GGGATGCCCC TCTCCAGCTT CCTGCTGAAG CCCATGCAGA GGATCACTCG CTACCCGCTG 
CTCATCCGAA GTATCCTGGA GAACACTCCA CAGAGTCATG TTGACCACTC CTCCCTGAAG 
CTGGCCCTAG AACGTGCTGA GGAGCTGTGC TCTCAGGTGA ACGAGGGAGT CCGGGAGAAG 
GAAAATTCAG ACCGGCTGGA GTGGATCCAG GCACACGTGC AGTGCGAAGG CTTGGCAGAG 
CAACTTATTT TCAACTCCCT CACCAACTGC CTGGGCCCCC GGAAGCTTCT GCACAGCGGG 
AAGCTGTACA AGACCAAGAG CAATAAGGAG CTGCACGCCT TCCTCTTCAA CGACTTCCTG 
CTGCTCACCT ACCTGGTCAG GCAGTTTGCC GCCGCCTCTG GCCACGAGAA GCTCTTCAAC 
TCCAAGTCCA GTGCTCAGTT CCGGATGTAC AAAACGCCCA TTTTCCTGAA TGAAGTGTTG 
GTGAAACTTC CCACAGACCC TTCCAGCGAT GAGCCCGTCT TCCACATTTC CCACATTGAT 
CGTGTGTACA CACTCCGAAC AGACAACATC AACGAGAGGA CGGCCTGGGT CCAGAAGATC 
AAGGGTGCCT CAGAGCAGTA CATCGACACT GAGAAGAAGA AACGGGAAAA GGCTTACCAA 
GCCCGTTCTC AAAAGACTTC AGGTATTGGG CGTCTGATGG TGCATGTCAT TGAAGCTACA 
GAATTAAAAG CCTGCAAACC AAACGGGAAA AGTAATCCAT ACTGTGAAGT CAGCATGGGC 
TCCCAAAGCT ATACCACCAG GACCCTGCAG GACACACTAA ACCCCAAGTG GAACTTCAAC 
TGCCAGTTCT TCATCAAGGA TCTTTACCAG GACGTTCTGT GTCTCACTAT GTTTGACAGA 
GACCAGTTTT CTCCAGATGA CTTCTTGGGT CGTACTGAAG TTCCAGTGGC AAAAATCCGA 
ACAGAACAGG AAAGCAAAGG CCCCACCACC CGCCGACTAC TACTGCACGA AGTCCCCACT 
GGAGAAGTCT GGGTCCGCTT TGACCTGCAA CTTTTTGAAC AAAAAACTCT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515024.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515087.1 Mus musculus intersectin 2 (Itsn2), transcript variant X2, mRNA.

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CloneID	OMu01935
Clone ID Related Accession (Same CDS sequence)	NM_001198968.2 , XM_006515024.3 , XM_030246627.1 , XM_006515023.3 , XM_006515023.4 , XM_006515024.4
Accession Version	XM_006515024.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515087.1
CDS	498..5555
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_006515024.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515024.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515023.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515086.1 Mus musculus intersectin 2 (Itsn2), transcript variant X1, mRNA.

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CloneID	OMu01935
Clone ID Related Accession (Same CDS sequence)	NM_001198968.2 , XM_006515024.3 , XM_030246627.1 , XM_006515023.3 , XM_006515023.4 , XM_006515024.4
Accession Version	XM_006515023.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515086.1
CDS	141..5198
Translation	MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_006515023.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515023.3

ORF Insert Sequence:

ATGATGGCTC AGTTTCCCAC AGCGATGAAT GGAGGGCCAA ATATGTGGGC TATTACATCT 
GAAGAACGTA CTAAGCATGA TAAACAGTTT GATAACCTCA AACCTTCAGG AGGTTACATA 
ACAGGTGATC AAGCCCGTAC TTTTTTCCTA CAGTCAGGTC TGCCGGCCCC GGTTTTAGCT 
GAAATATGGG CCTTATCAGA TCTGAACAAG GATGGGAAGA TGGACCAGCA AGAGTTCTCT 
ATAGCTATGA AACTCATCAA GTTAAAGTTG CAGGGCCAAC AGCTGCCTGT AGTCCTCCCT 
CCTATCATGA AACAACCCCC TATGTTCTCT CCACTAATCT CTGCTCGTTT TGGGATGGGA 
AGCATGCCCA ATCTGTCCAT TCATCAGCCA TTGCCTCCAG TTGCACCTAT AGCAACACCC 
TTGTCTTCTG CTACTTCAGG GACCAGTATT CCTCCCCTAA TGATGCCTGC TCCCCTAGTG 
CCTTCTGTTA GTACATCCTC ATTACCAAAT GGAACTGCCA GTCTCATTCA GCCTTTATCC 
ATTCCTTATT CTTCTTCAAC ATTGCCTCAT GCATCATCTT ACAGCCTGAT GATGGGAGGA 
TTTGGTGGTG CTAGTATCCA GAAGGCCCAG TCTCTGATTG ATTTAGGATC TAGTAGCTCA 
ACTTCCTCAA CTGCTTCCCT CTCAGGGAAC TCACCTAAGA CAGGGACCTC AGAGTGGGCA 
GTTCCTCAGC CTTCAAGATT AAAGTATCGG CAAAAATTTA ATAGTCTAGA CAAAGGCATG 
AGCGGATACC TCTCAGGTTT TCAAGCTAGA AATGCCCTTC TTCAGTCAAA TCTCTCTCAA 
ACTCAGCTAG CTACTATTTG GACTCTGGCT GACATCGATG GTGACGGACA GTTGAAAGCT 
GAAGAATTTA TTCTGGCGAT GCACCTCACT GACATGGCCA AAGCTGGACA GCCACTACCA 
CTGACGTTGC CTCCCGAGCT TGTCCCTCCA TCTTTCAGAG GGGGAAAGCA AGTTGATTCT 
GTTAATGGAA CTCTGCCTTC ATATCAGAAA ACACAAGAAG AAGAGCCTCA GAAGAAACTG 
CCAGTTACTT TTGAGGACAA ACGGAAAGCC AACTATGAAC GAGGAAACAT GGAGCTGGAG 
AAGCGACGCC AAGTGTTGAT GGAGCAGCAG CAGAGGGAGG CTGAACGCAA AGCCCAGAAA 
GAGAAGGAAG AGTGGGAGCG GAAACAGAGA GAACTGCAAG AGCAAGAATG GAAGAAGCAG 
CTGGAGTTGG AGAAACGCTT GGAGAAACAG AGAGAGCTGG AGAGACAGCG GGAGGAAGAG 
AGGAGAAAGG AGATAGAAAG ACGAGAGGCA GCAAAACAGG AGCTTGAGAG ACAACGCCGT 
TTAGAATGGG AAAGACTCCG TCGGCAGGAG CTGCTCAGTC AGAAGACCAG GGAACAAGAA 
GACATTGTCA GGCTGAGCTC CAGAAAGAAA AGTCTCCACC TGGAACTGGA AGCAGTGAAT 
GGAAAACATC AGCAGATCTC AGGCAGACTA CAAGATGTCC AAATCAGAAA GCAAACACAA 
AAGACTGAGC TAGAAGTTTT GGATAAACAG TGTGACCTGG AAATTATGGA AATCAAACAA 
CTTCAACAAG AGCTTAAGGA ATATCAAAAT AAGCTTATCT ATCTGGTCCC TGAGAAGCAG 
CTATTAAACG AAAGAATTAA AAACATGCAG CTCAGTAACA CACCTGATTC AGGGATCAGT 
TTACTTCATA AAAAGTCATC AGAAAAGGAA GAATTATGCC AAAGACTTAA AGAACAATTA 
GATGCTCTTG AAAAAGAAAC TGCATCTAAG CTCTCAGAAA TGGATTCATT TAACAATCAG 
CTGAAGTGTG GGAGTATGGA TGACTGTGTC CTTCAGTGCC TTTTGTCTCT GCTGAGCTGT 
CTCAACAACC TCTTCCTCTT ACTTAAGGAA CTCAGAGAAA GCTATAATAC ACAGCAGTTA 
GCCCTTGAAC AACTTCATAA AATCAAACGT GACAAATTGA AGGAAATCGA AAGAAAAAGA 
TTAGAGCAAA TTCAAAAAAA GAAACTAGAA GATGAGGCTG CAAGGAAAGC AAAGCAAGGA 
AAAGAAAACT TGTGGAGAGA AAGTATTAGA AAGGAAGAAG AGGAAAAGCA AAAACGACTC 
CAGGAAGAAA AGTCACAGGA CAAAACTCAA GAAGAGGAAC GAAAAGCTGA GGCAAAACAA 
AGTGAGACAG CCAGTGCTTT GGTGAATTAC AGAGCACTGT ACCCTTTTGA AGCAAGAAAC 
CATGATGAGA TGAGTTTTAG TTCTGGGGAT ATAATTCAGG TTGATGAAAA AACTGTAGGA 
GAGCCTGGTT GGCTTTATGG TAGTTTTCAG GGAAAGTTTG GCTGGTTCCC GTGCAACTAT 
GTAGAAAAAG TGCTGTCAAG TGAAAAAGCT CTGTCTCCTA AGAAGGCCTT ACTTCCTCCT 
ACAGTGTCTC TCTCTGCTAC CTCAACTTCT TCCCAGCCAC CAGCATCAGT GACTGATTAT 
CACAATGTAT CCTTCTCAAA CCTTACTGTT AATACAACAT GGCAGCAGAA GTCAGCTTTT 
ACCCGCACTG TGTCCCCTGG ATCTGTGTCC CCCATTCACG GACAGGGGCA GGCTGTAGAA 
AACCTGAAAG CCCAGGCCCT TTGTTCCTGG ACGGCAAAGA AGGAGAACCA CCTGAACTTC 
TCAAAGCACG ACGTCATCAC TGTCCTGGAG CAGCAGGAAA ACTGGTGGTT TGGGGAGGTG 
CACGGAGGAA GAGGATGGTT CCCCAAGTCT TATGTCAAGC TCATTCCTGG GAATGAAGTA 
CAGCGAGGAG AGCCAGAAGC TTTGTATGCA GCTGTGACTA AGAAACCTAC CTCCACAGCC 
TATCCAGTTA CCTCCACAGC CTATCCAGTT GGAGAAGACT ACATTGCACT TTATTCATAC 
TCAAGTGTAG AGCCCGGGGA TTTGACTTTC ACTGAAGGTG AAGAAATTCT AGTGACCCAG 
AAAGATGGAG AGTGGTGGAC AGGAAGTATT GGAGAGAGAA CTGGAATCTT CCCGTCCAAC 
TACGTCAGAC CAAAGGATCA AGAGAATTTT GGGAATGCTA GCAAATCTGG AGCATCAAAC 
AAAAAACCCG AGATCGCTCA AGTAACTTCA GCATATGCTG CTTCAGGGAC TGAGCAGCTC 
AGCCTTGCGC CAGGACAGTT AATATTAATC TTAAAGAAAA ACACAAGCGG GTGGTGGCAA 
GGAGAGCTAC AGGCCAGAGG GAAGAAACGA CAGAAGGGAT GGTTTCCTGC CAGCCATGTA 
AAGCTGCTAG GTCCAAGCAG TGAAAGAACC ATGCCTACTT TTCACGCTGT ATGTCAAGTG 
ATTGCTATGT ATGACTACAT GGCGAATAAC GAAGATGAGC TCAATTTCTC CAAAGGACAG 
CTGATTAATG TTATGAACAA AGATGACCCT GACTGGTGGC AAGGAGAAAC CAATGGTCTG 
ACTGGTCTCT TTCCTTCAAA CTATGTTAAG ATGACAACAG ACTCAGATCC AAGTCAACAG 
TGGTGTGCTG ACCTCCAAGC CCTGGACACA ATGCAGCCTA CGGAGAGGAA GCGACAGGGC 
TACATTCACG AGCTCATTCA GACAGAGGAG CGGTACATGG ACGACCTGCA GCTGGTCATC 
GAGGTCTTCC AGAAACGGAT GGCTGAGTCA GGCTTCCTCA CTGAAGCAGA CATGGCTCTG 
ATCTTTGTGA ACTGGAAAGA GCTCATCATG TCCAACACGA AGCTGCTGAG GGCCTTGCGG 
GTGAGGAAGA AGACTGGGGG TGAGAAGATG CCAGTTCAGA TGATTGGAGA CATCCTGGCG 
GCAGAGCTGT CCCACATGCA GGCCTACATC CGCTTCTGCA GCTGTCAGCT TAATGGGGCA 
ACCCTGTTAC AGCAGAAGAC AGACGAGGAC ACGGACTTCA AGGAATTTCT AAAGAAGTTG 
GCATCAGACC CACGATGCAA AGGGATGCCC CTCTCCAGCT TCCTGCTGAA GCCCATGCAG 
AGGATCACTC GCTACCCGCT GCTCATCCGA AGTATCCTGG AGAACACTCC ACAGAGTCAT 
GTTGACCACT CCTCCCTGAA GCTGGCCCTA GAACGTGCTG AGGAGCTGTG CTCTCAGGTG 
AACGAGGGAG TCCGGGAGAA GGAAAATTCA GACCGGCTGG AGTGGATCCA GGCACACGTG 
CAGTGCGAAG GCTTGGCAGA GCAACTTATT TTCAACTCCC TCACCAACTG CCTGGGCCCC 
CGGAAGCTTC TGCACAGCGG GAAGCTGTAC AAGACCAAGA GCAATAAGGA GCTGCACGCC 
TTCCTCTTCA ACGACTTCCT GCTGCTCACC TACCTGGTCA GGCAGTTTGC CGCCGCCTCT 
GGCCACGAGA AGCTCTTCAA CTCCAAGTCC AGTGCTCAGT TCCGGATGTA CAAAACGCCC 
ATTTTCCTGA ATGAAGTGTT GGTGAAACTT CCCACAGACC CTTCCAGCGA TGAGCCCGTC 
TTCCACATTT CCCACATTGA TCGTGTGTAC ACACTCCGAA CAGACAACAT CAACGAGAGG 
ACGGCCTGGG TCCAGAAGAT CAAGGGTGCC TCAGAGCAGT ACATCGACAC TGAGAAGAAG 
AAACGGGAAA AGGCTTACCA AGCCCGTTCT CAAAAGACTT CAGGTATTGG GCGTCTGATG 
GTGCATGTCA TTGAAGCTAC AGAATTAAAA GCCTGCAAAC CAAACGGGAA AAGTAATCCA 
TACTGTGAAG TCAGCATGGG CTCCCAAAGC TATACCACCA GGACCCTGCA GGACACACTA 
AACCCCAAGT GGAACTTCAA CTGCCAGTTC TTCATCAAGG ATCTTTACCA GGACGTTCTG 
TGTCTCACTA TGTTTGACAG AGACCAGTTT TCTCCAGATG ACTTCTTGGG TCGTACTGAA 
GTTCCAGTGG CAAAAATCCG AACAGAACAG GAAAGCAAAG GCCCCACCAC CCGCCGACTA 
CTACTGCACG AAGTCCCCAC TGGAGAAGTC TGGGTCCGCT TTGACCTGCA ACTTTTTGAA 
CAAAAAACTC TCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itsn2 ( XM_006515025.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515088.1 Mus musculus intersectin 2 (Itsn2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu47268
Clone ID Related Accession (Same CDS sequence)	XM_006515025.4 , XM_006515025.3
Accession Version	XM_006515025.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	intersectin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTA TTACATCTGA AGAACGTACT AAGCATGATA AACAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCCCGTACTT TTTTCCTACA GTCAGGTCTG 
CCGGCCCCGG TTTTAGCTGA AATATGGGCC TTATCAGATC TGAACAAGGA TGGGAAGATG 
GACCAGCAAG AGTTCTCTAT AGCTATGAAA CTCATCAAGT TAAAGTTGCA GGGCCAACAG 
CTGCCTGTAG TCCTCCCTCC TATCATGAAA CAACCCCCTA TGTTCTCTCC ACTAATCTCT 
GCTCGTTTTG GGATGGGAAG CATGCCCAAT CTGTCCATTC ATCAGCCATT GCCTCCAGTT 
GCACCTATAG CAACACCCTT GTCTTCTGCT ACTTCAGGGA CCAGTATTCC TCCCCTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGT ACATCCTCAT TACCAAATGG AACTGCCAGT 
CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATGC ATCATCTTAC 
AGCCTGATGA TGGGAGGATT TGGTGGTGCT AGTATCCAGA AGGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAACTC ACCTAAGACA 
GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AGTATCGGCA AAAATTTAAT 
AGTCTAGACA AAGGCATGAG CGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TCTCTCAAAC TCAGCTAGCT ACTATTTGGA CTCTGGCTGA CATCGATGGT 
GACGGACAGT TGAAAGCTGA AGAATTTATT CTGGCGATGC ACCTCACTGA CATGGCCAAA 
GCTGGACAGC CACTACCACT GACGTTGCCT CCCGAGCTTG TCCCTCCATC TTTCAGAGGG 
GGAAAGCAAG TTGATTCTGT TAATGGAACT CTGCCTTCAT ATCAGAAAAC ACAAGAAGAA 
GAGCCTCAGA AGAAACTGCC AGTTACTTTT GAGGACAAAC GGAAAGCCAA CTATGAACGA 
GGAAACATGG AGCTGGAGAA GCGACGCCAA GTGTTGATGG AGCAGCAGCA GAGGGAGGCT 
GAACGCAAAG CCCAGAAAGA GAAGGAAGAG TGGGAGCGGA AACAGAGAGA ACTGCAAGAG 
CAAGAATGGA AGAAGCAGCT GGAGTTGGAG AAACGCTTGG AGAAACAGAG AGAGCTGGAG 
AGACAGCGGG AGGAAGAGAG GAGAAAGGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAG 
CTTGAGAGAC AACGCCGTTT AGAATGGGAA AGACTCCGTC GGCAGGAGCT GCTCAGTCAG 
AAGACCAGGG AACAAGAAGA CATTGTCAGG CTGAGCTCCA GAAAGAAAAG TCTCCACCTG 
GAACTGGAAG CAGTGAATGG AAAACATCAG CAGATCTCAG GCAGACTACA AGATGTCCAA 
ATCAGAAAGC AAACACAAAA GACTGAGCTA GAAGTTTTGG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAACAACT TCAACAAGAG CTTAAGGAAT ATCAAAATAA GCTTATCTAT 
CTGGTCCCTG AGAAGCAGCT ATTAAACGAA AGAATTAAAA ACATGCAGCT CAGTAACACA 
CCTGATTCAG GGATCAGTTT ACTTCATAAA AAGTCATCAG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT CTCAGAAATG 
GATTCATTTA ACAATCAGCT GAAGTGTGGG AGTATGGATG ACTGTGTCCT TCAGTGCCTT 
TTGTCTCTGC TGAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT CAGAGAAAGC 
TATAATACAC AGCAGTTAGC CCTTGAACAA CTTCATAAAA TCAAACGTGA CAAATTGAAG 
GAAATCGAAA GAAAAAGATT AGAGCAAATT CAAAAAAAGA AACTAGAAGA TGAGGCTGCA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTG TGGAGAGAAA GTATTAGAAA GGAAGAAGAG 
GAAAAGCAAA AACGACTCCA GGAAGAAAAG TCACAGGACA AAACTCAAGA AGAGGAACGA 
AAAGCTGAGG CAAAACAAAG TGAGACAGCC AGTGCTTTGG TGAATTACAG AGCACTGTAC 
CCTTTTGAAG CAAGAAACCA TGATGAGATG AGTTTTAGTT CTGGGGATAT AATTCAGGTT 
GATGAAAAAA CTGTAGGAGA GCCTGGTTGG CTTTATGGTA GTTTTCAGGG AAAGTTTGGC 
TGGTTCCCGT GCAACTATGT AGAAAAAGTG CTGTCAAGTG AAAAAGCTCT GTCTCCTAAG 
AAGGCCTTAC TTCCTCCTAC AGTGTCTCTC TCTGCTACCT CAACTTCTTC CCAGCCACCA 
GCATCAGTGA CTGATTATCA CAATGTATCC TTCTCAAACC TTACTGTTAA TACAACATGG 
CAGCAGAAGT CAGCTTTTAC CCGCACTGTG TCCCCTGGAT CTGTGTCCCC CATTCACGGA 
CAGGGGCAGG CTGTAGAAAA CCTGAAAGCC CAGGCCCTTT GTTCCTGGAC GGCAAAGAAG 
GAGAACCACC TGAACTTCTC AAAGCACGAC GTCATCACTG TCCTGGAGCA GCAGGAAAAC 
TGGTGGTTTG GGGAGGTGCA CGGAGGAAGA GGATGGTTCC CCAAGTCTTA TGTCAAGCTC 
ATTCCTGGGA ATGAAGTACA GCGAGGAGAG CCAGAAGCTT TGTATGCAGC TGTGACTAAG 
AAACCTACCT CCACAGCCTA TCCAGTTACC TCCACAGCCT ATCCAGTTGG AGAAGACTAC 
ATTGCACTTT ATTCATACTC AAGTGTAGAG CCCGGGGATT TGACTTTCAC TGAAGGTGAA 
GAAATTCTAG TGACCCAGAA AGATGGAGAG TGGTGGACAG GAAGTATTGG AGAGAGAACT 
GGAATCTTCC CGTCCAACTA CGTCAGACCA AAGGATCAAG AGAATTTTGG GAATGCTAGC 
AAATCTGGAG CATCAAACAA AAAACCCGAG ATCGCTCAAG TAACTTCAGC ATATGCTGCT 
TCAGGGACTG AGCAGCTCAG CCTTGCGCCA GGACAGTTAA TATTAATCTT AAAGAAAAAC 
ACAAGCGGGT GGTGGCAAGG AGAGCTACAG GCCAGAGGGA AGAAACGACA GAAGGGATGG 
TTTCCTGCCA GCCATGTAAA GCTGCTAGGT CCAAGCAGTG AAAGAACCAT GCCTACTTTT 
CACGCTGTAT GTCAAGTGAT TGCTATGTAT GACTACATGG CGAATAACGA AGATGAGCTC 
AATTTCTCCA AAGGACAGCT GATTAATGTT ATGAACAAAG ATGACCCTGA CTGGTGGCAA 
GGAGAAACCA ATGGTCTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCAGATCCAA GTCAACAGTG GTGTGCTGAC CTCCAAGCCC TGGACACAAT GCAGCCTACG 
GAGAGGAAGC GACAGGGCTA CATTCACGAG CTCATTCAGA CAGAGGAGCG GTACATGGAC 
GACCTGCAGC TGGTCATCGA GGTCTTCCAG AAACGGATGG CTGAGTCAGG CTTCCTCACT 
GAAGCAGACA TGGCTCTGAT CTTTGTGAAC TGGAAAGAGC TCATCATGTC CAACACGAAG 
CTGCTGAGGG CCTTGCGGGT GAGGAAGAAG ACTGGGGGTG AGAAGATGCC AGTTCAGATG 
ATTGGAGACA TCCTGGCGGC AGAGCTGTCC CACATGCAGG CCTACATCCG CTTCTGCAGC 
TGTCAGCTTA ATGGGGCAAC CCTGTTACAG CAGAAGACAG ACGAGGACAC GGACTTCAAG 
GAATTTCTAA AGAAGTTGGC ATCAGACCCA CGATGCAAAG GGATGCCCCT CTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG GATCACTCGC TACCCGCTGC TCATCCGAAG TATCCTGGAG 
AACACTCCAC AGAGTCATGT TGACCACTCC TCCCTGAAGC TGGCCCTAGA ACGTGCTGAG 
GAGCTGTGCT CTCAGGTGAA CGAGGGAGTC CGGGAGAAGG AAAATTCAGA CCGGCTGGAG 
TGGATCCAGG CACACGTGCA GTGCGAAGGC TTGGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGCCCCCG GAAGCTTCTG CACAGCGGGA AGCTGTACAA GACCAAGAGC 
AATAAGGAGC TGCACGCCTT CCTCTTCAAC GACTTCCTGC TGCTCACCTA CCTGGTCAGG 
CAGTTTGCCG CCGCCTCTGG CCACGAGAAG CTCTTCAACT CCAAGTCCAG TGCTCAGTTC 
CGGATGTACA AAACGCCCAT TTTCCTGAAT GAAGTGTTGG TGAAACTTCC CACAGACCCT 
TCCAGCGATG AGCCCGTCTT CCACATTTCC CACATTGATC GTGTGTACAC ACTCCGAACA 
GACAACATCA ACGAGAGGAC GGCCTGGGTC CAGAAGATCA AGGGTGCCTC AGAGCAGTAC 
ATCGACACTG AGAAGAAGAA ACGGGAAAAG GCTTACCAAG CCCGTTCTCA AAAGACTTCA 
GGTATTGGGC GTCTGATGGT GCATGTCATT GAAGCTACAG AATTAAAAGC CTGCAAACCA 
AACGGGAAAA GTAATCCATA CTGTGAAGTC AGCATGGGCT CCCAAAGCTA TACCACCAGG 
ACCCTGCAGG ACACACTAAA CCCCAAGTGG AACTTCAACT GCCAGTTCTT CATCAAGGAT 
CTTTACCAGG ACGTTCTGTG TCTCACTATG TTTGACAGAG ACCAGTTTTC TCCAGATGAC 
TTCTTGGGTC GTACTGAAGT TCCAGTGGCA AAAATCCGAA CAGAACAGGA AAGCAAAGGC 
CCCACCACCC GCCGACTACT ACTGCACGAA GTCCCCACTG GAGAAGTCTG GGTCCGCTTT 
GACCTGCAAC TTTTTGAACA AAAAACTCTC CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515088.1
CDS	88..5103
Translation	MWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKKSLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQLLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGSMDDCVLQCLLSLLSCLNNLFLLLKELRESYNTQQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Target ORF information:

RefSeq Version	XM_006515025.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intersectin 2 (Itsn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515025.3

ORF Insert Sequence:

ATGTGGGCTA TTACATCTGA AGAACGTACT AAGCATGATA AACAGTTTGA TAACCTCAAA 
CCTTCAGGAG GTTACATAAC AGGTGATCAA GCCCGTACTT TTTTCCTACA GTCAGGTCTG 
CCGGCCCCGG TTTTAGCTGA AATATGGGCC TTATCAGATC TGAACAAGGA TGGGAAGATG 
GACCAGCAAG AGTTCTCTAT AGCTATGAAA CTCATCAAGT TAAAGTTGCA GGGCCAACAG 
CTGCCTGTAG TCCTCCCTCC TATCATGAAA CAACCCCCTA TGTTCTCTCC ACTAATCTCT 
GCTCGTTTTG GGATGGGAAG CATGCCCAAT CTGTCCATTC ATCAGCCATT GCCTCCAGTT 
GCACCTATAG CAACACCCTT GTCTTCTGCT ACTTCAGGGA CCAGTATTCC TCCCCTAATG 
ATGCCTGCTC CCCTAGTGCC TTCTGTTAGT ACATCCTCAT TACCAAATGG AACTGCCAGT 
CTCATTCAGC CTTTATCCAT TCCTTATTCT TCTTCAACAT TGCCTCATGC ATCATCTTAC 
AGCCTGATGA TGGGAGGATT TGGTGGTGCT AGTATCCAGA AGGCCCAGTC TCTGATTGAT 
TTAGGATCTA GTAGCTCAAC TTCCTCAACT GCTTCCCTCT CAGGGAACTC ACCTAAGACA 
GGGACCTCAG AGTGGGCAGT TCCTCAGCCT TCAAGATTAA AGTATCGGCA AAAATTTAAT 
AGTCTAGACA AAGGCATGAG CGGATACCTC TCAGGTTTTC AAGCTAGAAA TGCCCTTCTT 
CAGTCAAATC TCTCTCAAAC TCAGCTAGCT ACTATTTGGA CTCTGGCTGA CATCGATGGT 
GACGGACAGT TGAAAGCTGA AGAATTTATT CTGGCGATGC ACCTCACTGA CATGGCCAAA 
GCTGGACAGC CACTACCACT GACGTTGCCT CCCGAGCTTG TCCCTCCATC TTTCAGAGGG 
GGAAAGCAAG TTGATTCTGT TAATGGAACT CTGCCTTCAT ATCAGAAAAC ACAAGAAGAA 
GAGCCTCAGA AGAAACTGCC AGTTACTTTT GAGGACAAAC GGAAAGCCAA CTATGAACGA 
GGAAACATGG AGCTGGAGAA GCGACGCCAA GTGTTGATGG AGCAGCAGCA GAGGGAGGCT 
GAACGCAAAG CCCAGAAAGA GAAGGAAGAG TGGGAGCGGA AACAGAGAGA ACTGCAAGAG 
CAAGAATGGA AGAAGCAGCT GGAGTTGGAG AAACGCTTGG AGAAACAGAG AGAGCTGGAG 
AGACAGCGGG AGGAAGAGAG GAGAAAGGAG ATAGAAAGAC GAGAGGCAGC AAAACAGGAG 
CTTGAGAGAC AACGCCGTTT AGAATGGGAA AGACTCCGTC GGCAGGAGCT GCTCAGTCAG 
AAGACCAGGG AACAAGAAGA CATTGTCAGG CTGAGCTCCA GAAAGAAAAG TCTCCACCTG 
GAACTGGAAG CAGTGAATGG AAAACATCAG CAGATCTCAG GCAGACTACA AGATGTCCAA 
ATCAGAAAGC AAACACAAAA GACTGAGCTA GAAGTTTTGG ATAAACAGTG TGACCTGGAA 
ATTATGGAAA TCAAACAACT TCAACAAGAG CTTAAGGAAT ATCAAAATAA GCTTATCTAT 
CTGGTCCCTG AGAAGCAGCT ATTAAACGAA AGAATTAAAA ACATGCAGCT CAGTAACACA 
CCTGATTCAG GGATCAGTTT ACTTCATAAA AAGTCATCAG AAAAGGAAGA ATTATGCCAA 
AGACTTAAAG AACAATTAGA TGCTCTTGAA AAAGAAACTG CATCTAAGCT CTCAGAAATG 
GATTCATTTA ACAATCAGCT GAAGTGTGGG AGTATGGATG ACTGTGTCCT TCAGTGCCTT 
TTGTCTCTGC TGAGCTGTCT CAACAACCTC TTCCTCTTAC TTAAGGAACT CAGAGAAAGC 
TATAATACAC AGCAGTTAGC CCTTGAACAA CTTCATAAAA TCAAACGTGA CAAATTGAAG 
GAAATCGAAA GAAAAAGATT AGAGCAAATT CAAAAAAAGA AACTAGAAGA TGAGGCTGCA 
AGGAAAGCAA AGCAAGGAAA AGAAAACTTG TGGAGAGAAA GTATTAGAAA GGAAGAAGAG 
GAAAAGCAAA AACGACTCCA GGAAGAAAAG TCACAGGACA AAACTCAAGA AGAGGAACGA 
AAAGCTGAGG CAAAACAAAG TGAGACAGCC AGTGCTTTGG TGAATTACAG AGCACTGTAC 
CCTTTTGAAG CAAGAAACCA TGATGAGATG AGTTTTAGTT CTGGGGATAT AATTCAGGTT 
GATGAAAAAA CTGTAGGAGA GCCTGGTTGG CTTTATGGTA GTTTTCAGGG AAAGTTTGGC 
TGGTTCCCGT GCAACTATGT AGAAAAAGTG CTGTCAAGTG AAAAAGCTCT GTCTCCTAAG 
AAGGCCTTAC TTCCTCCTAC AGTGTCTCTC TCTGCTACCT CAACTTCTTC CCAGCCACCA 
GCATCAGTGA CTGATTATCA CAATGTATCC TTCTCAAACC TTACTGTTAA TACAACATGG 
CAGCAGAAGT CAGCTTTTAC CCGCACTGTG TCCCCTGGAT CTGTGTCCCC CATTCACGGA 
CAGGGGCAGG CTGTAGAAAA CCTGAAAGCC CAGGCCCTTT GTTCCTGGAC GGCAAAGAAG 
GAGAACCACC TGAACTTCTC AAAGCACGAC GTCATCACTG TCCTGGAGCA GCAGGAAAAC 
TGGTGGTTTG GGGAGGTGCA CGGAGGAAGA GGATGGTTCC CCAAGTCTTA TGTCAAGCTC 
ATTCCTGGGA ATGAAGTACA GCGAGGAGAG CCAGAAGCTT TGTATGCAGC TGTGACTAAG 
AAACCTACCT CCACAGCCTA TCCAGTTACC TCCACAGCCT ATCCAGTTGG AGAAGACTAC 
ATTGCACTTT ATTCATACTC AAGTGTAGAG CCCGGGGATT TGACTTTCAC TGAAGGTGAA 
GAAATTCTAG TGACCCAGAA AGATGGAGAG TGGTGGACAG GAAGTATTGG AGAGAGAACT 
GGAATCTTCC CGTCCAACTA CGTCAGACCA AAGGATCAAG AGAATTTTGG GAATGCTAGC 
AAATCTGGAG CATCAAACAA AAAACCCGAG ATCGCTCAAG TAACTTCAGC ATATGCTGCT 
TCAGGGACTG AGCAGCTCAG CCTTGCGCCA GGACAGTTAA TATTAATCTT AAAGAAAAAC 
ACAAGCGGGT GGTGGCAAGG AGAGCTACAG GCCAGAGGGA AGAAACGACA GAAGGGATGG 
TTTCCTGCCA GCCATGTAAA GCTGCTAGGT CCAAGCAGTG AAAGAACCAT GCCTACTTTT 
CACGCTGTAT GTCAAGTGAT TGCTATGTAT GACTACATGG CGAATAACGA AGATGAGCTC 
AATTTCTCCA AAGGACAGCT GATTAATGTT ATGAACAAAG ATGACCCTGA CTGGTGGCAA 
GGAGAAACCA ATGGTCTGAC TGGTCTCTTT CCTTCAAACT ATGTTAAGAT GACAACAGAC 
TCAGATCCAA GTCAACAGTG GTGTGCTGAC CTCCAAGCCC TGGACACAAT GCAGCCTACG 
GAGAGGAAGC GACAGGGCTA CATTCACGAG CTCATTCAGA CAGAGGAGCG GTACATGGAC 
GACCTGCAGC TGGTCATCGA GGTCTTCCAG AAACGGATGG CTGAGTCAGG CTTCCTCACT 
GAAGCAGACA TGGCTCTGAT CTTTGTGAAC TGGAAAGAGC TCATCATGTC CAACACGAAG 
CTGCTGAGGG CCTTGCGGGT GAGGAAGAAG ACTGGGGGTG AGAAGATGCC AGTTCAGATG 
ATTGGAGACA TCCTGGCGGC AGAGCTGTCC CACATGCAGG CCTACATCCG CTTCTGCAGC 
TGTCAGCTTA ATGGGGCAAC CCTGTTACAG CAGAAGACAG ACGAGGACAC GGACTTCAAG 
GAATTTCTAA AGAAGTTGGC ATCAGACCCA CGATGCAAAG GGATGCCCCT CTCCAGCTTC 
CTGCTGAAGC CCATGCAGAG GATCACTCGC TACCCGCTGC TCATCCGAAG TATCCTGGAG 
AACACTCCAC AGAGTCATGT TGACCACTCC TCCCTGAAGC TGGCCCTAGA ACGTGCTGAG 
GAGCTGTGCT CTCAGGTGAA CGAGGGAGTC CGGGAGAAGG AAAATTCAGA CCGGCTGGAG 
TGGATCCAGG CACACGTGCA GTGCGAAGGC TTGGCAGAGC AACTTATTTT CAACTCCCTC 
ACCAACTGCC TGGGCCCCCG GAAGCTTCTG CACAGCGGGA AGCTGTACAA GACCAAGAGC 
AATAAGGAGC TGCACGCCTT CCTCTTCAAC GACTTCCTGC TGCTCACCTA CCTGGTCAGG 
CAGTTTGCCG CCGCCTCTGG CCACGAGAAG CTCTTCAACT CCAAGTCCAG TGCTCAGTTC 
CGGATGTACA AAACGCCCAT TTTCCTGAAT GAAGTGTTGG TGAAACTTCC CACAGACCCT 
TCCAGCGATG AGCCCGTCTT CCACATTTCC CACATTGATC GTGTGTACAC ACTCCGAACA 
GACAACATCA ACGAGAGGAC GGCCTGGGTC CAGAAGATCA AGGGTGCCTC AGAGCAGTAC 
ATCGACACTG AGAAGAAGAA ACGGGAAAAG GCTTACCAAG CCCGTTCTCA AAAGACTTCA 
GGTATTGGGC GTCTGATGGT GCATGTCATT GAAGCTACAG AATTAAAAGC CTGCAAACCA 
AACGGGAAAA GTAATCCATA CTGTGAAGTC AGCATGGGCT CCCAAAGCTA TACCACCAGG 
ACCCTGCAGG ACACACTAAA CCCCAAGTGG AACTTCAACT GCCAGTTCTT CATCAAGGAT 
CTTTACCAGG ACGTTCTGTG TCTCACTATG TTTGACAGAG ACCAGTTTTC TCCAGATGAC 
TTCTTGGGTC GTACTGAAGT TCCAGTGGCA AAAATCCGAA CAGAACAGGA AAGCAAAGGC 
CCCACCACCC GCCGACTACT ACTGCACGAA GTCCCCACTG GAGAAGTCTG GGTCCGCTTT 
GACCTGCAAC TTTTTGAACA AAAAACTCTC CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Itsn2
Entrez Gene ID	20403
Full Name	intersectin 2
Synonyms	AI327390,Ese2,Sh3d1B,Sh3p18,mKIAA1256
General protein information	Preferred Names intersectin 2 Names intersectin-2 EH and SH3 domains protein 2 EH domain and SH3 domain regulator of endocytosis 2 Eh domain, SH3 domain regulator of endocytosis 2 SH3 domain protein 1B SH3 domain-containing protein 1B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1

	LOC704858	XP_002808077.1
Rattus norvegicus (Norway rat)	Itsn2	XP_003750174.1
Mus musculus (house mouse)	Itsn2	NP_001185897.1
Gallus gallus (chicken)	ITSN2	XP_419989.4
Pan troglodytes (chimpanzee)	ITSN2	XP_001145237.3
Canis lupus familiaris (dog)	ITSN2	XP_849291.2
Danio rerio (zebrafish)	itsn2a	NP_001138272.1
Xenopus tropicalis (tropical clawed frog)	itsn2	XP_004918242.1
Homo sapiens (human)	ITSN2	NP_062541.3
Bos taurus (cattle)	ITSN2	XP_002691512.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Itsn2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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