Sema4d cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema4d
Entrez Gene ID	20354
Full Name	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Synonyms	CD100,Semacl2,Semaj,Semcl2,coll-4
General protein information	Preferred Names sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D Names semaphorin-4D M-sema G sema J semaphorin H semaphorin-C-like 2 semaphorin-J
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A5

mRNA and Protein(s)

mRNA	Protein	Name
XM_030247216.1	XP_030103076.1	semaphorin-4D isoform X1
NM_001281880.1	NP_001268809.1	semaphorin-4D precursor
NM_013660.4	NP_038688.2	semaphorin-4D precursor
XM_006516891.3	XP_006516954.1	semaphorin-4D isoform X1
XM_030247214.1	XP_030103074.1	semaphorin-4D isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sema4d	NP_001164034.1
Homo sapiens (human)	SEMA4D	NP_006369.3
Pan troglodytes (chimpanzee)	SEMA4D	XP_001141771.2
Canis lupus familiaris (dog)	SEMA4D	XP_005616015.1
Gallus gallus (chicken)	SEMA4D	XP_424426.4
Danio rerio (zebrafish)	sema4d	XP_003200859.2
	SEMA4D	XP_001090890.1
Mus musculus (house mouse)	Sema4d	NP_038688.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SEMA4D-/- neutrophils show impaired NET formation when in contact with endothelial cells.
Title: Semaphorin 4D inhibits neutrophil activation and is involved in the pathogenesis of neutrophil-mediated autoimmune vasculitis.

These results reveal a novel signaling network, the Sema4D-RhoA-Akt signal cascade, that coordinates cellular function and morphology and highlights the importance of specific spatiotemporally restricted components of a signaling pathway in the regulation of ameloblast differentiation.
Title: The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination With Cell Polarization During Ameloblast Differentiation.

our findings revealed for the first time that Sema4D positively regulated both the adaptive and innate immune responses in contact hypersensitivity
Title: Sema4D is required in both the adaptive and innate immune responses of contact hypersensitivity.

Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice
Title: Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice.

Sema4D-deficiency affected ischemia-induced microglial activation in morphology, iNOS expression, and proliferation, and this deficiency most likely resulted in moderation of cytotoxicity and locomotor behavioral abnormality after cerebral ischemia
Title: Effect of Sema4D on microglial function in middle cerebral artery occlusion mice.

The following Sema4d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema4d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13736	NM_013660.4 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu13736	NM_001281880.1 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48522	XM_006516891.3 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu13736	XM_030247216.1 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13736	XM_030247214.1 Latest version!	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema4d ( NM_001281880.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268809.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13736
Clone ID Related Accession (Same CDS sequence)	XM_030247216.1 , NM_001281880.1 , NM_013660.4 , XM_030247214.1
Accession Version	NM_001281880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	semaphorin-4D precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126247.3, AK132688.1, AC126547.4 and BE692125.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132688.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268809.1
CDS	598..3183
Translation	MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNRLLMSLLLFIFVLFLCLFSYNCYKGYLPGQCLKFRSALLLGKKTPKSDFSDLEQSVKETLVEPGSFSQQNGDHPKPALDTGYETEQDTITSKVPTDREDSQRIDELSARDKPFDVKCELKFADSDADGD

Target ORF information:

RefSeq Version	NM_001281880.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281880.1

ORF Insert Sequence:

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4d ( NM_013660.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038688.2 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13736
Clone ID Related Accession (Same CDS sequence)	XM_030247216.1 , NM_001281880.1 , NM_013660.4 , XM_030247214.1
Accession Version	NM_013660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	semaphorin-4D precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126247.3, AK132688.1, AC126547.4 and BE692125.1. On Aug 9, 2013 this sequence version replaced NM_013660.3. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049780.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038688.2
CDS	534..3119
Translation	MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNRLLMSLLLFIFVLFLCLFSYNCYKGYLPGQCLKFRSALLLGKKTPKSDFSDLEQSVKETLVEPGSFSQQNGDHPKPALDTGYETEQDTITSKVPTDREDSQRIDELSARDKPFDVKCELKFADSDADGD

Target ORF information:

RefSeq Version	NM_013660.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013660.4

ORF Insert Sequence:

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4d ( XM_006516891.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516954.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X10, mRNA.

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CloneID	OMu48522
Clone ID Related Accession (Same CDS sequence)	XM_006516891.3
Accession Version	XM_006516891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	semaphorin-4D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGCCTCATC TCCTAAGTCC 
TTCCCTCCTC CTGGCTCCTC TCTATCCTGT CTGGGCCCTG ACTCATCTTC TCCCAGAAGC 
CCTCGGCCGG CCTTGGCCTC GGGGCCCAAC AGCCGTAAGC AGGTCACCTT ACTGCCACCC 
TTCTTGAGTG ACCAGGCGCA GAAGGTGCAG GCGCTGGGGA CCTTCTACCT CTTCTGCCAG 
GCCACAGGTC CAGCAGATAT CCGATTCATT TGGGAGAAGA ACAGGCATGC CCTGGAGACG 
TGTGTACCTA TGCAGACCCA TGCACTGCCC GATGGCAGGG CCCATGCCCT CAGCTGGCTT 
CGGGACACCA TCCAAGAAAC CACCGAATAC AGCTGCTCTG TCCTCTCCTC AGCAGGAAAC 
CAGACTTCAA AGGTTCACAT CACTGTGATG AGACACGAGG CACTGCAGCA GGAAAAGTGG 
AGCCAGGAAC TTGCTACGTG GCGTGCTGTG GCCGGGGAGC ATGACAGGCT GATGCAGGGC 
TGGAGGAAGG CATGGGAAAG CTGCAGCAAG GATACCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516954.1
CDS	530..2731
Translation	MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLASSPKSFPPPGSSLSCLGPDSSSPRSPRPALASGPNSRKQVTLLPPFLSDQAQKVQALGTFYLFCQATGPADIRFIWEKNRHALETCVPMQTHALPDGRAHALSWLRDTIQETTEYSCSVLSSAGNQTSKVHITVMRHEALQQEKWSQELATWRAVAGEHDRLMQGWRKAWESCSKDTF

Target ORF information:

RefSeq Version	XM_006516891.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516891.3

ORF Insert Sequence:

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGCCTCATC TCCTAAGTCC 
TTCCCTCCTC CTGGCTCCTC TCTATCCTGT CTGGGCCCTG ACTCATCTTC TCCCAGAAGC 
CCTCGGCCGG CCTTGGCCTC GGGGCCCAAC AGCCGTAAGC AGGTCACCTT ACTGCCACCC 
TTCTTGAGTG ACCAGGCGCA GAAGGTGCAG GCGCTGGGGA CCTTCTACCT CTTCTGCCAG 
GCCACAGGTC CAGCAGATAT CCGATTCATT TGGGAGAAGA ACAGGCATGC CCTGGAGACG 
TGTGTACCTA TGCAGACCCA TGCACTGCCC GATGGCAGGG CCCATGCCCT CAGCTGGCTT 
CGGGACACCA TCCAAGAAAC CACCGAATAC AGCTGCTCTG TCCTCTCCTC AGCAGGAAAC 
CAGACTTCAA AGGTTCACAT CACTGTGATG AGACACGAGG CACTGCAGCA GGAAAAGTGG 
AGCCAGGAAC TTGCTACGTG GCGTGCTGTG GCCGGGGAGC ATGACAGGCT GATGCAGGGC 
TGGAGGAAGG CATGGGAAAG CTGCAGCAAG GATACCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4d ( XM_030247216.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103076.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X2, mRNA.

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CloneID	OMu13736
Clone ID Related Accession (Same CDS sequence)	XM_030247216.1 , NM_001281880.1 , NM_013660.4 , XM_030247214.1
Accession Version	XM_030247216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-4D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103076.1
CDS	1133..3718
Translation	MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNRLLMSLLLFIFVLFLCLFSYNCYKGYLPGQCLKFRSALLLGKKTPKSDFSDLEQSVKETLVEPGSFSQQNGDHPKPALDTGYETEQDTITSKVPTDREDSQRIDELSARDKPFDVKCELKFADSDADGD

Target ORF information:

RefSeq Version	XM_030247216.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247216.1

ORF Insert Sequence:

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4d ( XM_030247214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103074.1 Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X1, mRNA.

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CloneID	OMu13736
Clone ID Related Accession (Same CDS sequence)	XM_030247216.1 , NM_001281880.1 , NM_013660.4 , XM_030247214.1
Accession Version	XM_030247214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	semaphorin-4D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103074.1
CDS	536..3121
Translation	MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNRLLMSLLLFIFVLFLCLFSYNCYKGYLPGQCLKFRSALLLGKKTPKSDFSDLEQSVKETLVEPGSFSQQNGDHPKPALDTGYETEQDTITSKVPTDREDSQRIDELSARDKPFDVKCELKFADSDADGD

Target ORF information:

RefSeq Version	XM_030247214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247214.1

ORF Insert Sequence:

ATGAGGATGT GTGCCCCCGT TAGGGGGCTG TTCTTGGCCC TGGTGGTAGT GTTGAGAACC 
GCGGTGGCAT TTGCACCTGT GCCTCGGCTC ACCTGGGAAC ATGGAGAGGT AGGTCTGGTG 
CAGTTTCACA AGCCAGGCAT CTTTAACTAC TCGGCCTTGC TGATGAGTGA GGACAAAGAC 
ACTCTGTATG TAGGCGCCCG GGAAGCAGTC TTTGCAGTGA ATGCGCTGAA CATCTCTGAG 
AAGCAACATG AGGTATATTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG TGCAGAGAAG 
GGGAAATCAA AGCAGACGGA ATGCCTAAAC TACATTCGAG TACTACAGCC ACTAAGCAGC 
ACTTCCCTCT ATGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG 
ACATCCTTCA AGTTTCTGGG GAAAAGTGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC 
GCCCACAGCT ACACATCAGT CATGGTTGGG GGCGAGCTCT ACTCTGGGAC GTCCTATAAT 
TTCTTGGGCA GTGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCTT GAGGACGGAG 
TATGCCATCC CGTGGCTGAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCAGAAAAGC 
CCAGATGGTC CGGAGGGTGA AGATGACAAG GTCTACTTCT TTTTTACGGA GGTATCCGTG 
GAGTACGAAT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC 
CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGGCTGATC 
TGCTCCAAGC CAGACAGTGG CCTGGTCTTC AACATACTTC AGGATGTGTT TGTGCTGAGG 
GCCCCGGGCC TCAAGGAGCC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAATGTG 
GGTCTGTCAG CGGTGTGCGC CTACACACTG GCCACGGTGG AGGCAGTCTT CTCCCGTGGA 
AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTGCG CTACAATGGC 
CCAGTGCCCA CTCCCCGACC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC 
ACCAGCTCCT TGAATCTCCC AGACAAAACA CTGCAGTTTG TAAAAGACCA CCCTTTGATG 
GATGACTCAG TGACCCCGAT AGACAACAGA CCCAAGCTGA TCAAAAAAGA TGTAAACTAC 
ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CTTTCTACGA CGTCATGTTC 
ATCAGCACAG ACCGGGGAGC TCTGCATAAA GCAGTCATCC TCACAAAAGA GGTGCATGTC 
ATCGAGGAGA CCCAACTCTT CCGGGACTCT GAACCGGTCC TAACTCTGCT GCTATCGTCA 
AAGAAGGGGA GGAAGTTTGT CTATGCAGGC TCCAACTCTG GAGTGGTCCA AGCGCCCCTG 
GCATTCTGCG AAAAGCACGG TAGCTGTGAA GACTGTGTGT TAGCACGGGA CCCCTACTGT 
GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCCTGCACC AGGAAGAGGC CTCCAGCAGG 
GGCTGGATTC AGGACATGAG CGGTGACACA TCCTCATGCC TGGATAAGAG TAAAGAAAGT 
TTCAACCAGC ATTTTTTCAA GCACGGCGGC ACAGCGGAAC TCAAATGTTT CCAAAAGTCC 
AACCTAGCCC GGGTGGTATG GAAGTTCCAG AATGGCGAGT TGAAGGCCGC AAGTCCCAAG 
TACGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTCAACC TGTCGGACGG AGACAGCGGC 
GTGTACCAGT GCCTGTCAGA GGAAAGGGTG AGGAATAAAA CGGTCTCCCA GCTGCTGGCC 
AAGCACGTTC TGGAAGTGAA GATGGTACCT CGGACCCCCC CCTCACCTAC CTCAGAGGAT 
GCTCAGACAG AAGGTAGTAA GATCACATCC AAAATGCCGG TTGCATCTAC CCAGGGGTCC 
TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGAG CCGCCACCCT ACCTCCCAAG 
TCCTCCTCCG GCACATCCTG TGAACCAAAG ATGGTCATCA ACACGGTCCC CCAGCTCCAC 
TCAGAGAAGA CGGTGTATCT CAAGTCCAGT GACAACCGCC TGCTCATGTC TCTCCTCCTC 
TTCATCTTTG TCCTCTTCCT CTGCCTCTTT TCCTACAACT GCTACAAGGG CTACCTGCCC 
GGACAGTGCT TAAAATTCCG CTCAGCCCTG CTGCTTGGAA AGAAAACACC CAAGTCAGAC 
TTCTCTGACC TGGAGCAGAG TGTGAAGGAG ACACTGGTCG AGCCTGGGAG CTTCTCCCAG 
CAGAACGGCG ACCACCCCAA GCCAGCCCTG GATACGGGCT ATGAAACGGA GCAGGACACC 
ATCACCAGCA AAGTCCCCAC GGATCGTGAG GACTCGCAAC GGATCGATGA ACTCTCTGCC 
CGGGACAAAC CGTTTGATGT CAAGTGTGAA CTGAAGTTTG CAGATTCGGA TGCTGACGGG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

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Sema4d cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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