Cxcl12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cxcl12
Entrez Gene ID	20315
Full Name	chemokine (C-X-C motif) ligand 12
Synonyms	Pbsf,Scyb12,Sdf1,Tlsf,Tpar1
General protein information	Preferred Names chemokine (C-X-C motif) ligand 12 Names stromal cell-derived factor 1 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 pre-B-cell growth-stimulating factor thymic lymphoma cell-stimulating factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 54.81 cM\|6 F1
Summary	This gene encodes a member of the alpha chemokine protein family. The encoded protein is secreted and functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4. The encoded protein plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013655.4	NP_038683.1	stromal cell-derived factor 1 isoform beta precursor
NM_021704.3	NP_068350.1	stromal cell-derived factor 1 isoform alpha precursor
NM_001012477.2	NP_001012495.1	stromal cell-derived factor 1 isoform gamma precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CXCL12	NP_001171605.1
Rattus norvegicus (Norway rat)	Cxcl12	NP_001029055.1
Mus musculus (house mouse)	Cxcl12	NP_001012495.1
Gallus gallus (chicken)	CXCL12	NP_989841.1
Bos taurus (cattle)	CXCL12	NP_001106645.1
Pan troglodytes (chimpanzee)	LOC741624	XP_001157443.2
	CXCL12	NP_001028106.1
Canis lupus familiaris (dog)	CXCL12	NP_001121569.1
Xenopus tropicalis (tropical clawed frog)	cxcl12	NP_001015764.1

Gene Ontology
Bibliography

Related articles in PubMed

The multiple faces of CXCL12 (SDF-1alpha) in the regulation of immunity during health and disease.
Karin N
Journal of leukocyte biology88(3)463-73(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest that PECAM-1 enhances SDF-1-induced chemotaxis by augmenting and prolonging activation of the PI3K/Akt/mTORC1 pathway and Rap1 and that PECAM-1, at least partly, exerts its activity by inhibiting SDF-1-induced internalization of CXCR4
Title: Molecular mechanisms for enhancement of stromal cell-derived factor 1-induced chemotaxis by platelet endothelial cell adhesion molecule 1 (PECAM-1).

High Cxcl12 expression is associated with Prostate Cancer.
Title: Proinflammatory CXCL12-CXCR4/CXCR7 Signaling Axis Drives Myc-Induced Prostate Cancer in Obese Mice.

Authors demonstrate that targeting the SDF-1/CXCR4 pathway in the context of KLF10 deletion substantially suppresses PDAC progression
Title: KLF10 loss in the pancreas provokes activation of SDF-1 and induces distant metastases of pancreatic ductal adenocarcinoma in the Kras

CD26-cleavage skews CXCL12 towards beta-arrestin dependent recruitment through ACKR3 and destroys the CXCR4-mediated lymphocyte chemoattractant properties of CXCL12 in vivo
Title: Truncation of CXCL12 by CD26 reduces its CXC chemokine receptor 4- and atypical chemokine receptor 3-dependent activity on endothelial cells and lymphocytes.

endothelial CXCR7+ cells regulate CXCL12 gradient direction by controlling concentrations near but not far from the vasculature.
Title: The CXCL12/CXCR7 signaling axis, isoforms, circadian rhythms, and tumor cellular composition dictate gradients in tissue.

The following Cxcl12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cxcl12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22834	NM_021704.3 Latest version!	Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu22845	NM_001012477.2 Latest version!	Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu23034	NM_013655.4 Latest version!	Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cxcl12 ( NM_021704.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_068350.1 Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22834
Clone ID Related Accession (Same CDS sequence)	NM_021704.3
Accession Version	NM_021704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Mus musculus(house mouse)
Product	stromal cell-derived factor 1 isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC155646.4 and AK045092.1. On Dec 8, 2009 this sequence version replaced NM_021704.2. Transcript Variant: This variant (1) differs in the 3' UTR and 3' coding region compared to variant 3. The resulting isoform (alpha) is shorter at the C-terminus, compared to isoform gamma. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF151999.1, D43804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCCA AGGTCGTCGC CGTGCTGGCC CTGGTGCTGG CCGCGCTCTG CATCAGTGAC 
GGTAAACCAG TCAGCCTGAG CTACCGATGC CCCTGCCGGT TCTTCGAGAG CCACATCGCC 
AGAGCCAACG TCAAGCATCT GAAAATCCTC AACACTCCAA ACTGTGCCCT TCAGATTGTT 
GCACGGCTGA AGAACAACAA CAGACAAGTG TGCATTGACC CGAAATTAAA GTGGATCCAA 
GAGTACCTGG AGAAAGCTTT AAACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068350.1
CDS	122..391
Translation	MDAKVVAVLALVLAALCISDGKPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

Target ORF information:

RefSeq Version	NM_021704.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021704.3

ORF Insert Sequence:

ATGGACGCCA AGGTCGTCGC CGTGCTGGCC CTGGTGCTGG CCGCGCTCTG CATCAGTGAC 
GGTAAACCAG TCAGCCTGAG CTACCGATGC CCCTGCCGGT TCTTCGAGAG CCACATCGCC 
AGAGCCAACG TCAAGCATCT GAAAATCCTC AACACTCCAA ACTGTGCCCT TCAGATTGTT 
GCACGGCTGA AGAACAACAA CAGACAAGTG TGCATTGACC CGAAATTAAA GTGGATCCAA 
GAGTACCTGG AGAAAGCTTT AAACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cxcl12 ( NM_001012477.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001012495.1 Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22845
Clone ID Related Accession (Same CDS sequence)	NM_001012477.2
Accession Version	NM_001012477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	stromal cell-derived factor 1 isoform gamma precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC155646.4 and BC046827.1. On Dec 8, 2009 this sequence version replaced NM_001012477.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (gamma). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046827.1, SRR1660819.192626.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCCA AGGTCGTCGC CGTGCTGGCC CTGGTGCTGG CCGCGCTCTG CATCAGTGAC 
GGTAAACCAG TCAGCCTGAG CTACCGATGC CCCTGCCGGT TCTTCGAGAG CCACATCGCC 
AGAGCCAACG TCAAGCATCT GAAAATCCTC AACACTCCAA ACTGTGCCCT TCAGATTGTT 
GCACGGCTGA AGAACAACAA CAGACAAGTG TGCATTGACC CGAAATTAAA GTGGATCCAA 
GAGTACCTGG AGAAAGCTTT AAACAAGGGG CGCAGAGAAG AAAAAGTGGG GAAAAAAGAA 
AAGATAGGAA AAAAGAAGCG ACAGAAGAAG AGAAAGGCTG CCCAGAAAAG GAAAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012495.1
CDS	122..481
Translation	MDAKVVAVLALVLAALCISDGKPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKGRREEKVGKKEKIGKKKRQKKRKAAQKRKN

Target ORF information:

RefSeq Version	NM_001012477.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012477.2

ORF Insert Sequence:

ATGGACGCCA AGGTCGTCGC CGTGCTGGCC CTGGTGCTGG CCGCGCTCTG CATCAGTGAC 
GGTAAACCAG TCAGCCTGAG CTACCGATGC CCCTGCCGGT TCTTCGAGAG CCACATCGCC 
AGAGCCAACG TCAAGCATCT GAAAATCCTC AACACTCCAA ACTGTGCCCT TCAGATTGTT 
GCACGGCTGA AGAACAACAA CAGACAAGTG TGCATTGACC CGAAATTAAA GTGGATCCAA 
GAGTACCTGG AGAAAGCTTT AAACAAGGGG CGCAGAGAAG AAAAAGTGGG GAAAAAAGAA 
AAGATAGGAA AAAAGAAGCG ACAGAAGAAG AGAAAGGCTG CCCAGAAAAG GAAAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cxcl12 ( NM_013655.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038683.1 Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23034
Clone ID Related Accession (Same CDS sequence)	NM_013655.4
Accession Version	NM_013655.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	stromal cell-derived factor 1 isoform beta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC155646.4 and BB840704.1. On Dec 8, 2009 this sequence version replaced NM_013655.3. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region compared to variant 3. The resulting isoform (beta) is shorter and has a distinct C-terminus compared to isoform gamma. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157553.1, D43805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCCA AGGTCGTCGC CGTGCTGGCC CTGGTGCTGG CCGCGCTCTG CATCAGTGAC 
GGTAAACCAG TCAGCCTGAG CTACCGATGC CCCTGCCGGT TCTTCGAGAG CCACATCGCC 
AGAGCCAACG TCAAGCATCT GAAAATCCTC AACACTCCAA ACTGTGCCCT TCAGATTGTT 
GCACGGCTGA AGAACAACAA CAGACAAGTG TGCATTGACC CGAAATTAAA GTGGATCCAA 
GAGTACCTGG AGAAAGCTTT AAACAAGAGG CTCAAGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038683.1
CDS	122..403
Translation	MDAKVVAVLALVLAALCISDGKPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKRLKM

Target ORF information:

RefSeq Version	NM_013655.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013655.4

ORF Insert Sequence:

ATGGACGCCA AGGTCGTCGC CGTGCTGGCC CTGGTGCTGG CCGCGCTCTG CATCAGTGAC 
GGTAAACCAG TCAGCCTGAG CTACCGATGC CCCTGCCGGT TCTTCGAGAG CCACATCGCC 
AGAGCCAACG TCAAGCATCT GAAAATCCTC AACACTCCAA ACTGTGCCCT TCAGATTGTT 
GCACGGCTGA AGAACAACAA CAGACAAGTG TGCATTGACC CGAAATTAAA GTGGATCCAA 
GAGTACCTGG AGAAAGCTTT AAACAAGAGG CTCAAGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The multiple faces of CXCL12 (SDF-1alpha) in the regulation of immunity during health and disease.
Journal of leukocyte biology88(3)463-73(2010 Sep)
Karin N

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Cxcl12 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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