Polr1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr1b
Entrez Gene ID	20017
Full Name	polymerase (RNA) I polypeptide B
Synonyms	128kDa,D630020H17Rik,RPA116,RPA135,RPA2,Rpo1-2
General protein information	Preferred Names polymerase (RNA) I polypeptide B Names DNA-directed RNA polymerase I subunit RPA2 DNA-directed RNA polymerase I 135 kDa polypeptide DNA-directed RNA polymerase I 135kDa polypeptide RNA polymerase 1-2, 128 kDa subunit RNA polymerase I subunit 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_009086.2	NP_033112.2	DNA-directed RNA polymerase I subunit RPA2
XM_006499026.2	XP_006499089.1	DNA-directed RNA polymerase I subunit RPA2 isoform X2
XM_006499025.4	XP_006499088.1	DNA-directed RNA polymerase I subunit RPA2 isoform X1
XM_006499025.3	XP_006499088.1	DNA-directed RNA polymerase I subunit RPA2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR1B	NP_001093762.1
Rattus norvegicus (Norway rat)	Polr1b	NP_113961.1
Drosophila melanogaster (fruit fly)	RpI135	NP_476708.1
Eremothecium gossypii	AGOS_ABR029W	NP_982975.2
Pan troglodytes (chimpanzee)	POLR1B	XP_001145399.1
Canis lupus familiaris (dog)	POLR1B	XP_003639648.1
Saccharomyces cerevisiae (baker's yeast)	RPA135	NP_015335.1
Neurospora crassa	NCU08616	XP_964824.1
Mus musculus (house mouse)	Polr1b	NP_033112.2
Kluyveromyces lactis	KLLA0B06303g	XP_451816.1
Homo sapiens (human)	POLR1B	NP_001269701.1
Gallus gallus (chicken)	POLR1B	XP_004935307.1
Caenorhabditis elegans (roundworm)	F14B4.3	NP_492476.1
Xenopus tropicalis (tropical clawed frog)	polr1b	XP_002940442.1
	POLR1B	XP_001088474.1
Danio rerio (zebrafish)	polr1b	NP_956812.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009539	XP_310152.4
Schizosaccharomyces pombe (fission yeast)	rpa2	NP_595819.2
Magnaporthe oryzae (rice blast fungus)	MGG_01572	XP_003714550.1
Arabidopsis thaliana (thale cress)	NRPA2	NP_564341.2

Gene Ontology
Bibliography

Related articles in PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

Mechanical loading induces the expression of a Pol I regulon at the onset of skeletal muscle hypertrophy.
von Walden F, Casagrande V, ?stlund Farrants AK, Nader GA
American journal of physiology. Cell physiology302(10)C1523-30(2012 May)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene.
Chen H, Li Z, Haruna K, Li Z, Li Z, Semba K, Araki M, Yamamura K, Araki K
Biochemical and biophysical research communications365(4)636-42(2008 Jan)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA
BMC developmental biology514(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased transcription and protein expression of polymerase I regulon is consistent with the increase in ribosomal RNA following skeletal muscle hypertrophy.
Title: Mechanical loading induces the expression of a Pol I regulon at the onset of skeletal muscle hypertrophy.

These results indicate that the loss of rDNA transcription induced nucleolar structure disorganization and apoptosis in preimplantation embryos.
Title: Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene.

The following Polr1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30176	XM_006499026.2 Latest version!	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu30175	XM_006499025.4 Latest version!	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu03493	NM_009086.2 Latest version!	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu30175	XM_006499025.3	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr1b ( XM_006499026.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499089.1 Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30176
Clone ID Related Accession (Same CDS sequence)	XM_006499026.2
Accession Version	XM_006499026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006499026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGCT GGGTGGATAA GGACCTTGCT CCTGAAGTTG CAGACACTCT CCGTCGATTC 
AAGGTGTTGA GAGAAAAAAG AATTCCTCCC TGGATGGAGG TGGCCCTGAT TCCCATGACA 
GGAAAACCAA GTCTGTACCC AGGTCTGTTC CTCTTTACCA CTCCCTGCAG GCTGGTGAGG 
CCTGTGCAGA ACTTGGAACT GGGCAGAGAA GAGCTCATTG GAACCATGGA ACAGCTCTTC 
ATGAACGTCG CCATCTTTGA GGACGAGGTG TTTGGTGGCA TCTCCACACA CCAGGAGCTC 
TTCCCTCACA GCCTGCTGAG CGTGATCGCC AACTTCATCC CCTTCTCGGA TCACAACCAG 
AGTCCTCGGA ACATGTACCA GTGCCAGATG GGTAAGCAGA CCATGGGCTT TCCGCTGCTC 
ACTTACCAAA ACCGATCAGA TAATAAACTC TATCGACTCC AAACCCCTCA GAGCCCTCTA 
GTGAGACCGT GCATGTATGA TTTCTATGAC ATGGACAACT ATCCGATCGG GACAAACGCC 
ATTGTAGCTG TGATCTCCTA CACTGGCTAT GATATGGAGG ACGCCATGAT TGTCAACAAG 
GCTTCCTGGG AACGAGGCTT CGCTCATGGA AGCGTCTACA AGTCTGAGTT CATAGACCTC 
TCAGAGAAGT TTAAGCAGGG AGAGGATAAC CTGGTGTTCG GGGTCAAGCC TGGTGACCCT 
CGGGTGATGC AGAAGCTGGA CGATGATGGG TTGCCATTCA TAGGCGCAAA GCTGGAGTAT 
GGCGACCCTT ACTACAGCTA CCTCAACCTT AACACTGGGG AAGGCTTCGT GGTTTACTAC 
AAGAGTAAAG AAAACTGTGT TGTGGACAAC ATCAAAGTGT GTAGTAACGA CATGGGCAGT 
GGGAAGTTCA AGTGTATCTG CATCACAGTC CGCATCCCCC GGAACCCAAC CATTGGAGAT 
AAGTTTGCCA GCCGACATGG TCAGAAGGGC ATTTTGAGCA GATTGTGGCC AGCCGAGGAC 
ATGCCTTTCA CTGAGAGTGG CATGATGCCG GACATTCTGT TTAATCCTCA CGGGTTTCCC 
TCCCGTATGA CCATAGGTAT GTTAATCGAG AGCATGGCTG GGAAGTCTGC AGCTTTGCAC 
GGTCTCTGCC ATGATGCTAC ACCCTTCATC TTCTCCGAGG AGAACTCTGC CTTAGAGTAC 
TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC AACTTCTACG GCACCGAGAG ACTGTATAGT 
GGCATCAGTG GGATGGAGCT GGAGGCTGAC ATCTTCATTG GTGTGGTTTA TTACCAGCGC 
TTACGACACA TGGTGTCAGA CAAATTTCAA GTCAGAACAA CTGGAGCCAG GGACAAAGTC 
ACCAACCAGC CCCTCGGGGG ACGAAATGTT CAGGGTGGAA TCCGCTTTGG GGAGATGGAG 
CGGGATGCTC TGTTGGCACA CGGTACGTCT TTCCTTCTTC ATGACCGGCT TTTCAACTGC 
TCTGACCGTT CTGTCGCTCA CATGTGTGTA GAGTGTGGCA GTTTGCTTTC TCCGCTACTT 
GAGAAGCCTC CTCCGTCTTG GTCTGCGATG CGCAACAGAA AGTACAACTG CACTGTCTGT 
GGCCGCAGTG ACACCATCGA CACCGTCTCT GTGCCGTATG TCTTCCGGTA CTTTGTAGCT 
GAGCTGGCAG CCATGAACAT CAAAGTGAAA CTGGATGTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499089.1
CDS	161..1885
Translation	MVGWVDKDLAPEVADTLRRFKVLREKRIPPWMEVALIPMTGKPSLYPGLFLFTTPCRLVRPVQNLELGREELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGEDNLVFGVKPGDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVYYKSKENCVVDNIKVCSNDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVI

Target ORF information:

RefSeq Version	XM_006499026.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499026.2

ORF Insert Sequence:

ATGGTGGGCT GGGTGGATAA GGACCTTGCT CCTGAAGTTG CAGACACTCT CCGTCGATTC 
AAGGTGTTGA GAGAAAAAAG AATTCCTCCC TGGATGGAGG TGGCCCTGAT TCCCATGACA 
GGAAAACCAA GTCTGTACCC AGGTCTGTTC CTCTTTACCA CTCCCTGCAG GCTGGTGAGG 
CCTGTGCAGA ACTTGGAACT GGGCAGAGAA GAGCTCATTG GAACCATGGA ACAGCTCTTC 
ATGAACGTCG CCATCTTTGA GGACGAGGTG TTTGGTGGCA TCTCCACACA CCAGGAGCTC 
TTCCCTCACA GCCTGCTGAG CGTGATCGCC AACTTCATCC CCTTCTCGGA TCACAACCAG 
AGTCCTCGGA ACATGTACCA GTGCCAGATG GGTAAGCAGA CCATGGGCTT TCCGCTGCTC 
ACTTACCAAA ACCGATCAGA TAATAAACTC TATCGACTCC AAACCCCTCA GAGCCCTCTA 
GTGAGACCGT GCATGTATGA TTTCTATGAC ATGGACAACT ATCCGATCGG GACAAACGCC 
ATTGTAGCTG TGATCTCCTA CACTGGCTAT GATATGGAGG ACGCCATGAT TGTCAACAAG 
GCTTCCTGGG AACGAGGCTT CGCTCATGGA AGCGTCTACA AGTCTGAGTT CATAGACCTC 
TCAGAGAAGT TTAAGCAGGG AGAGGATAAC CTGGTGTTCG GGGTCAAGCC TGGTGACCCT 
CGGGTGATGC AGAAGCTGGA CGATGATGGG TTGCCATTCA TAGGCGCAAA GCTGGAGTAT 
GGCGACCCTT ACTACAGCTA CCTCAACCTT AACACTGGGG AAGGCTTCGT GGTTTACTAC 
AAGAGTAAAG AAAACTGTGT TGTGGACAAC ATCAAAGTGT GTAGTAACGA CATGGGCAGT 
GGGAAGTTCA AGTGTATCTG CATCACAGTC CGCATCCCCC GGAACCCAAC CATTGGAGAT 
AAGTTTGCCA GCCGACATGG TCAGAAGGGC ATTTTGAGCA GATTGTGGCC AGCCGAGGAC 
ATGCCTTTCA CTGAGAGTGG CATGATGCCG GACATTCTGT TTAATCCTCA CGGGTTTCCC 
TCCCGTATGA CCATAGGTAT GTTAATCGAG AGCATGGCTG GGAAGTCTGC AGCTTTGCAC 
GGTCTCTGCC ATGATGCTAC ACCCTTCATC TTCTCCGAGG AGAACTCTGC CTTAGAGTAC 
TTTGGTGAGA TGTTAAAGGC TGCTGGCTAC AACTTCTACG GCACCGAGAG ACTGTATAGT 
GGCATCAGTG GGATGGAGCT GGAGGCTGAC ATCTTCATTG GTGTGGTTTA TTACCAGCGC 
TTACGACACA TGGTGTCAGA CAAATTTCAA GTCAGAACAA CTGGAGCCAG GGACAAAGTC 
ACCAACCAGC CCCTCGGGGG ACGAAATGTT CAGGGTGGAA TCCGCTTTGG GGAGATGGAG 
CGGGATGCTC TGTTGGCACA CGGTACGTCT TTCCTTCTTC ATGACCGGCT TTTCAACTGC 
TCTGACCGTT CTGTCGCTCA CATGTGTGTA GAGTGTGGCA GTTTGCTTTC TCCGCTACTT 
GAGAAGCCTC CTCCGTCTTG GTCTGCGATG CGCAACAGAA AGTACAACTG CACTGTCTGT 
GGCCGCAGTG ACACCATCGA CACCGTCTCT GTGCCGTATG TCTTCCGGTA CTTTGTAGCT 
GAGCTGGCAG CCATGAACAT CAAAGTGAAA CTGGATGTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr1b ( XM_006499025.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499088.1 Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30175
Clone ID Related Accession (Same CDS sequence)	XM_006499025.4 , XM_006499025.3
Accession Version	XM_006499025.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCG ACGGCCGGTG GCGGAACCTG CCCAGCGGGC CCAGCCTAAA GCATTTAACC 
GACCCCTCGT ACGGGATTCC CCCAGAGCAG CAAAAGGCAG CTTTGCAGGA CCTGACGCGG 
GCGCACGTGG ACTCCTTCAA CTACGCGGCG CTGGAGGGGC TGAGCCACGC GGTGCAGGCC 
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGCATAT CTCTTACCAT TGTGGATGCT 
GTCATCAGTC CACCCTCAGT GCCCAAAGGG ACCATCTGCA AAGATCTCAA CGTTTATCCA 
GCTGAGTGTC GTGGCCGAAA GAGCACCTAC CGAGGGAGGC TGACGGCTGA TATCAGCTGG 
GCCGTGAATG GAGTCCCCAA AGGGATCATT AAACAGTTTC TCGGCTATGT TCCCATCATG 
GTGAAGTCCA AGCTTTGCAA CTTATACAAC CTTCCTCCTC GAGTCCTCAT TGAGCACCAT 
GAGGAGGCAG AGGAAATGGG CGGTTATTTT ATAATCAATG GCATAGAAAA AGTCATCCGC 
ATGTTGATTG TGCCCCGGAG AAATTTCCCC ATCGCCATGG TAAGGCCGAA ATGGAAAAGC 
AGAGGACTCG GCTACACTCA GTTTGGGGTC TCCATGCGCT GTGTGAGAGA AGAGCACTCC 
GCCGTGAACA TGAACCTGCA CTATGTGGAG AACGGCACGG TTATGTTAAA CTTTATTTAC 
CGCAAAGAGC TCTTTTTCCT TCCTTTGGGA TTTGCACTTA AGGCACTCGT GAGCTTTTCT 
GATTATCAGA TTTTCCAGGA GCTTATCAAG GGCAAAGAGG AGGACTCTTT CTTTAGGAAT 
TCTGTTTCTC AGATGCTGCG GATTGTCATA GAAGAGGGCT GTCACTCACA GAAGCAGGTT 
CTTAACTATC TGGGAGAATG CTTCCGAGTG AAGCTCAGCC TTCCTGATTG GTACCCTAAC 
GTGGAAGCTG CCGAGTTCCT GCTTAACCAG TGCATCTGCA TCCACCTGCA GTCCAACACT 
GACAAGTTCT ACCTGCTCTG CCTCATGACC CGGAAGCTGT TCGCTTTAGC CAGAGGGGAG 
TGCATGGACG ACAACCCTGA TAGCCTAGTG AACCAAGAGG TCCTCAGCCC TGGGCAGCTC 
TTCCTGATGT TTCTGAAGGA AAAAATGGAA AACTGGCTAG TGTCTATTAA AATAGTTTTA 
GATAAGAGGG CTCAGAAGGC CAATGTTTCC ATAAACAACG AAAATTTGAT GAAGATTTTT 
AGTATGGGAA CAGAGCTAAC AAGACCATTT GAATATCTTC TTGCTACTGG GAATCTGCGT 
TCTAAAACAG GTCTCGGCTT CCTGCAAGAT TCTGGCCTGT GTGTTGTGGC TGACAAGCTG 
AACTTCCTTC GCTACCTCTC CCATTTCCGC TGTGTGCACA GAGGGGCTGC CTTTGCCAAG 
ATGAGGACCA CCACCGTGCG CAGGCTGCTG CCAGAATCCT GGGGCTTCCT CTGCCCTGTG 
CACACTCCAG ACGGGGCGCC TTGTGGGCTG TTGAACCACT TGACTGCCGT GTGTGAGGAG 
TCACTCCTGT TGATACAGCG CCATGTCGAC CGTACAGTGA CTGCTACCCT GTCCTGCTGG 
ATGGCGTCAT GGTGGGCTGG GTGGATAAGG ACCTTGCTCC TGAAGTTGCA GACACTCTCC 
GTCGATTCAA GGCTGGTGAG GCCTGTGCAG AACTTGGAAC TGGGCAGAGA AGAGCTCATT 
GGAACCATGG AACAGCTCTT CATGAACGTC GCCATCTTTG AGGACGAGGT GTTTGGTGGC 
ATCTCCACAC ACCAGGAGCT CTTCCCTCAC AGCCTGCTGA GCGTGATCGC CAACTTCATC 
CCCTTCTCGG ATCACAACCA GAGTCCTCGG AACATGTACC AGTGCCAGAT GGGTAAGCAG 
ACCATGGGCT TTCCGCTGCT CACTTACCAA AACCGATCAG ATAATAAACT CTATCGACTC 
CAAACCCCTC AGAGCCCTCT AGTGAGACCG TGCATGTATG ATTTCTATGA CATGGACAAC 
TATCCGATCG GGACAAACGC CATTGTAGCT GTGATCTCCT ACACTGGCTA TGATATGGAG 
GACGCCATGA TTGTCAACAA GGCTTCCTGG GAACGAGGCT TCGCTCATGG AAGCGTCTAC 
AAGTCTGAGT TCATAGACCT CTCAGAGAAG TTTAAGCAGG GAGAGGATAA CCTGGTGTTC 
GGGGTCAAGC CTGGTGACCC TCGGGTGATG CAGAAGCTGG ACGATGATGG GTTGCCATTC 
ATAGGCGCAA AGCTGGAGTA TGGCGACCCT TACTACAGCT ACCTCAACCT TAACACTGGG 
GAAGGCTTCG TGGTTTACTA CAAGAGTAAA GAAAACTGTG TTGTGGACAA CATCAAAGTG 
TGTAGTAACG ACATGGGCAG TGGGAAGTTC AAGTGTATCT GCATCACAGT CCGCATCCCC 
CGGAACCCAA CCATTGGAGA TAAGTTTGCC AGCCGACATG GTCAGAAGGG CATTTTGAGC 
AGATTGTGGC CAGCCGAGGA CATGCCTTTC ACTGAGAGTG GCATGATGCC GGACATTCTG 
TTTAATCCTC ACGGGTTTCC CTCCCGTATG ACCATAGGTA TGTTAATCGA GAGCATGGCT 
GGGAAGTCTG CAGCTTTGCA CGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCCGAG 
GAGAACTCTG CCTTAGAGTA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAACTTCTAC 
GGCACCGAGA GACTGTATAG TGGCATCAGT GGGATGGAGC TGGAGGCTGA CATCTTCATT 
GGTGTGGTTT ATTACCAGCG CTTACGACAC ATGGTGTCAG ACAAATTTCA AGTCAGAACA 
ACTGGAGCCA GGGACAAAGT CACCAACCAG CCCCTCGGGG GACGAAATGT TCAGGGTGGA 
ATCCGCTTTG GGGAGATGGA GCGGGATGCT CTGTTGGCAC ACGGTACGTC TTTCCTTCTT 
CATGACCGGC TTTTCAACTG CTCTGACCGT TCTGTCGCTC ACATGTGTGT AGAGTGTGGC 
AGTTTGCTTT CTCCGCTACT TGAGAAGCCT CCTCCGTCTT GGTCTGCGAT GCGCAACAGA 
AAGTACAACT GCACTGTCTG TGGCCGCAGT GACACCATCG ACACCGTCTC TGTGCCGTAT 
GTCTTCCGGT ACTTTGTAGC TGAGCTGGCA GCCATGAACA TCAAAGTGAA ACTGGATGTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499088.1
CDS	116..3361
Translation	MDVDGRWRNLPSGPSLKHLTDPSYGIPPEQQKAALQDLTRAHVDSFNYAALEGLSHAVQAIPPFEFAFKDERISLTIVDAVISPPSVPKGTICKDLNVYPAECRGRKSTYRGRLTADISWAVNGVPKGIIKQFLGYVPIMVKSKLCNLYNLPPRVLIEHHEEAEEMGGYFIINGIEKVIRMLIVPRRNFPIAMVRPKWKSRGLGYTQFGVSMRCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFRNSVSQMLRIVIEEGCHSQKQVLNYLGECFRVKLSLPDWYPNVEAAEFLLNQCICIHLQSNTDKFYLLCLMTRKLFALARGECMDDNPDSLVNQEVLSPGQLFLMFLKEKMENWLVSIKIVLDKRAQKANVSINNENLMKIFSMGTELTRPFEYLLATGNLRSKTGLGFLQDSGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLLPESWGFLCPVHTPDGAPCGLLNHLTAVCEESLLLIQRHVDRTVTATLSCWMASWWAGWIRTLLLKLQTLSVDSRLVRPVQNLELGREELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGEDNLVFGVKPGDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVYYKSKENCVVDNIKVCSNDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVI

Target ORF information:

RefSeq Version	XM_006499025.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499025.4

ORF Insert Sequence:

ATGGATGTCG ACGGCCGGTG GCGGAACCTG CCCAGCGGGC CCAGCCTAAA GCATTTAACC 
GACCCCTCGT ACGGGATTCC CCCAGAGCAG CAAAAGGCAG CTTTGCAGGA CCTGACGCGG 
GCGCACGTGG ACTCCTTCAA CTACGCGGCG CTGGAGGGGC TGAGCCACGC GGTGCAGGCC 
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGCATAT CTCTTACCAT TGTGGATGCT 
GTCATCAGTC CACCCTCAGT GCCCAAAGGG ACCATCTGCA AAGATCTCAA CGTTTATCCA 
GCTGAGTGTC GTGGCCGAAA GAGCACCTAC CGAGGGAGGC TGACGGCTGA TATCAGCTGG 
GCCGTGAATG GAGTCCCCAA AGGGATCATT AAACAGTTTC TCGGCTATGT TCCCATCATG 
GTGAAGTCCA AGCTTTGCAA CTTATACAAC CTTCCTCCTC GAGTCCTCAT TGAGCACCAT 
GAGGAGGCAG AGGAAATGGG CGGTTATTTT ATAATCAATG GCATAGAAAA AGTCATCCGC 
ATGTTGATTG TGCCCCGGAG AAATTTCCCC ATCGCCATGG TAAGGCCGAA ATGGAAAAGC 
AGAGGACTCG GCTACACTCA GTTTGGGGTC TCCATGCGCT GTGTGAGAGA AGAGCACTCC 
GCCGTGAACA TGAACCTGCA CTATGTGGAG AACGGCACGG TTATGTTAAA CTTTATTTAC 
CGCAAAGAGC TCTTTTTCCT TCCTTTGGGA TTTGCACTTA AGGCACTCGT GAGCTTTTCT 
GATTATCAGA TTTTCCAGGA GCTTATCAAG GGCAAAGAGG AGGACTCTTT CTTTAGGAAT 
TCTGTTTCTC AGATGCTGCG GATTGTCATA GAAGAGGGCT GTCACTCACA GAAGCAGGTT 
CTTAACTATC TGGGAGAATG CTTCCGAGTG AAGCTCAGCC TTCCTGATTG GTACCCTAAC 
GTGGAAGCTG CCGAGTTCCT GCTTAACCAG TGCATCTGCA TCCACCTGCA GTCCAACACT 
GACAAGTTCT ACCTGCTCTG CCTCATGACC CGGAAGCTGT TCGCTTTAGC CAGAGGGGAG 
TGCATGGACG ACAACCCTGA TAGCCTAGTG AACCAAGAGG TCCTCAGCCC TGGGCAGCTC 
TTCCTGATGT TTCTGAAGGA AAAAATGGAA AACTGGCTAG TGTCTATTAA AATAGTTTTA 
GATAAGAGGG CTCAGAAGGC CAATGTTTCC ATAAACAACG AAAATTTGAT GAAGATTTTT 
AGTATGGGAA CAGAGCTAAC AAGACCATTT GAATATCTTC TTGCTACTGG GAATCTGCGT 
TCTAAAACAG GTCTCGGCTT CCTGCAAGAT TCTGGCCTGT GTGTTGTGGC TGACAAGCTG 
AACTTCCTTC GCTACCTCTC CCATTTCCGC TGTGTGCACA GAGGGGCTGC CTTTGCCAAG 
ATGAGGACCA CCACCGTGCG CAGGCTGCTG CCAGAATCCT GGGGCTTCCT CTGCCCTGTG 
CACACTCCAG ACGGGGCGCC TTGTGGGCTG TTGAACCACT TGACTGCCGT GTGTGAGGAG 
TCACTCCTGT TGATACAGCG CCATGTCGAC CGTACAGTGA CTGCTACCCT GTCCTGCTGG 
ATGGCGTCAT GGTGGGCTGG GTGGATAAGG ACCTTGCTCC TGAAGTTGCA GACACTCTCC 
GTCGATTCAA GGCTGGTGAG GCCTGTGCAG AACTTGGAAC TGGGCAGAGA AGAGCTCATT 
GGAACCATGG AACAGCTCTT CATGAACGTC GCCATCTTTG AGGACGAGGT GTTTGGTGGC 
ATCTCCACAC ACCAGGAGCT CTTCCCTCAC AGCCTGCTGA GCGTGATCGC CAACTTCATC 
CCCTTCTCGG ATCACAACCA GAGTCCTCGG AACATGTACC AGTGCCAGAT GGGTAAGCAG 
ACCATGGGCT TTCCGCTGCT CACTTACCAA AACCGATCAG ATAATAAACT CTATCGACTC 
CAAACCCCTC AGAGCCCTCT AGTGAGACCG TGCATGTATG ATTTCTATGA CATGGACAAC 
TATCCGATCG GGACAAACGC CATTGTAGCT GTGATCTCCT ACACTGGCTA TGATATGGAG 
GACGCCATGA TTGTCAACAA GGCTTCCTGG GAACGAGGCT TCGCTCATGG AAGCGTCTAC 
AAGTCTGAGT TCATAGACCT CTCAGAGAAG TTTAAGCAGG GAGAGGATAA CCTGGTGTTC 
GGGGTCAAGC CTGGTGACCC TCGGGTGATG CAGAAGCTGG ACGATGATGG GTTGCCATTC 
ATAGGCGCAA AGCTGGAGTA TGGCGACCCT TACTACAGCT ACCTCAACCT TAACACTGGG 
GAAGGCTTCG TGGTTTACTA CAAGAGTAAA GAAAACTGTG TTGTGGACAA CATCAAAGTG 
TGTAGTAACG ACATGGGCAG TGGGAAGTTC AAGTGTATCT GCATCACAGT CCGCATCCCC 
CGGAACCCAA CCATTGGAGA TAAGTTTGCC AGCCGACATG GTCAGAAGGG CATTTTGAGC 
AGATTGTGGC CAGCCGAGGA CATGCCTTTC ACTGAGAGTG GCATGATGCC GGACATTCTG 
TTTAATCCTC ACGGGTTTCC CTCCCGTATG ACCATAGGTA TGTTAATCGA GAGCATGGCT 
GGGAAGTCTG CAGCTTTGCA CGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCCGAG 
GAGAACTCTG CCTTAGAGTA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAACTTCTAC 
GGCACCGAGA GACTGTATAG TGGCATCAGT GGGATGGAGC TGGAGGCTGA CATCTTCATT 
GGTGTGGTTT ATTACCAGCG CTTACGACAC ATGGTGTCAG ACAAATTTCA AGTCAGAACA 
ACTGGAGCCA GGGACAAAGT CACCAACCAG CCCCTCGGGG GACGAAATGT TCAGGGTGGA 
ATCCGCTTTG GGGAGATGGA GCGGGATGCT CTGTTGGCAC ACGGTACGTC TTTCCTTCTT 
CATGACCGGC TTTTCAACTG CTCTGACCGT TCTGTCGCTC ACATGTGTGT AGAGTGTGGC 
AGTTTGCTTT CTCCGCTACT TGAGAAGCCT CCTCCGTCTT GGTCTGCGAT GCGCAACAGA 
AAGTACAACT GCACTGTCTG TGGCCGCAGT GACACCATCG ACACCGTCTC TGTGCCGTAT 
GTCTTCCGGT ACTTTGTAGC TGAGCTGGCA GCCATGAACA TCAAAGTGAA ACTGGATGTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr1b ( NM_009086.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033112.2 Mus musculus polymerase (RNA) I polypeptide B (Polr1b), mRNA.

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CloneID	OMu03493
Clone ID Related Accession (Same CDS sequence)	NM_009086.2
Accession Version	NM_009086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB860575.1, AK143501.1 and U58280.1. On Mar 13, 2009 this sequence version replaced NM_009086.1. ##Evidence-Data-START## Transcript exon combination :: U58280.1, AK144616.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGTCG ACGGCCGGTG GCGGAACCTG CCCAGCGGGC CCAGCCTAAA GCATTTAACC 
GACCCCTCGT ACGGGATTCC CCCAGAGCAG CAAAAGGCAG CTTTGCAGGA CCTGACGCGG 
GCGCACGTGG ACTCCTTCAA CTACGCGGCG CTGGAGGGGC TGAGCCACGC GGTGCAGGCC 
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGCATAT CTCTTACCAT TGTGGATGCT 
GTCATCAGTC CACCCTCAGT GCCCAAAGGG ACCATCTGCA AAGATCTCAA CGTTTATCCA 
GCTGAGTGTC GTGGCCGAAA GAGCACCTAC CGAGGGAGGC TGACGGCTGA TATCAGCTGG 
GCCGTGAATG GAGTCCCCAA AGGGATCATT AAACAGTTTC TCGGCTATGT TCCCATCATG 
GTGAAGTCCA AGCTTTGCAA CTTATACAAC CTTCCTCCTC GAGTCCTCAT TGAGCACCAT 
GAGGAGGCAG AGGAAATGGG CGGTTATTTT ATAATCAATG GCATAGAAAA AGTCATCCGC 
ATGTTGATTG TGCCCCGGAG AAATTTCCCC ATCGCCATGG TAAGGCCGAA ATGGAAAAGC 
AGAGGACTCG GCTACACTCA GTTTGGGGTC TCCATGCGCT GTGTGAGAGA AGAGCACTCC 
GCCGTGAACA TGAACCTGCA CTATGTGGAG AACGGCACGG TTATGTTAAA CTTTATTTAC 
CGCAAAGAGC TCTTTTTCCT TCCTTTGGGA TTTGCACTTA AGGCACTCGT GAGCTTTTCT 
GATTATCAGA TTTTCCAGGA GCTTATCAAG GGCAAAGAGG AGGACTCTTT CTTTAGGAAT 
TCTGTTTCTC AGATGCTGCG GATTGTCATA GAAGAGGGCT GTCACTCACA GAAGCAGGTT 
CTTAACTATC TGGGAGAATG CTTCCGAGTG AAGCTCAGCC TTCCTGATTG GTACCCTAAC 
GTGGAAGCTG CCGAGTTCCT GCTTAACCAG TGCATCTGCA TCCACCTGCA GTCCAACACT 
GACAAGTTCT ACCTGCTCTG CCTCATGACC CGGAAGCTGT TCGCTTTAGC CAGAGGGGAG 
TGCATGGACG ACAACCCTGA TAGCCTAGTG AACCAAGAGG TCCTCAGCCC TGGGCAGCTC 
TTCCTGATGT TTCTGAAGGA AAAAATGGAA AACTGGCTAG TGTCTATTAA AATAGTTTTA 
GATAAGAGGG CTCAGAAGGC CAATGTTTCC ATAAACAACG AAAATTTGAT GAAGATTTTT 
AGTATGGGAA CAGAGCTAAC AAGACCATTT GAATATCTTC TTGCTACTGG GAATCTGCGT 
TCTAAAACAG GTCTCGGCTT CCTGCAAGAT TCTGGCCTGT GTGTTGTGGC TGACAAGCTG 
AACTTCCTTC GCTACCTCTC CCATTTCCGC TGTGTGCACA GAGGGGCTGC CTTTGCCAAG 
ATGAGGACCA CCACCGTGCG CAGGCTGCTG CCAGAATCCT GGGGCTTCCT CTGCCCTGTG 
CACACTCCAG ACGGGGCGCC TTGTGGGCTG TTGAACCACT TGACTGCCGT GTGTGAGGTT 
GTTACCAAGT TTGTGTACAC GGCATCTATT CCAGCCTTGC TCTGTGGCTT AGGAGTCACT 
CCTGTTGATA CAGCGCCATG TCGACCGTAC AGTGACTGCT ACCCTGTCCT GCTGGATGGC 
GTCATGGTGG GCTGGGTGGA TAAGGACCTT GCTCCTGAAG TTGCAGACAC TCTCCGTCGA 
TTCAAGGTGT TGAGAGAAAA AAGAATTCCT CCCTGGATGG AGGTGGCCCT GATTCCCATG 
ACAGGAAAAC CAAGTCTGTA CCCAGGTCTG TTCCTCTTTA CCACTCCCTG CAGGCTGGTG 
AGGCCTGTGC AGAACTTGGA ACTGGGCAGA GAAGAGCTCA TTGGAACCAT GGAACAGCTC 
TTCATGAACG TCGCCATCTT TGAGGACGAG GTGTTTGGTG GCATCTCCAC ACACCAGGAG 
CTCTTCCCTC ACAGCCTGCT GAGCGTGATC GCCAACTTCA TCCCCTTCTC GGATCACAAC 
CAGAGTCCTC GGAACATGTA CCAGTGCCAG ATGGGTAAGC AGACCATGGG CTTTCCGCTG 
CTCACTTACC AAAACCGATC AGATAATAAA CTCTATCGAC TCCAAACCCC TCAGAGCCCT 
CTAGTGAGAC CGTGCATGTA TGATTTCTAT GACATGGACA ACTATCCGAT CGGGACAAAC 
GCCATTGTAG CTGTGATCTC CTACACTGGC TATGATATGG AGGACGCCAT GATTGTCAAC 
AAGGCTTCCT GGGAACGAGG CTTCGCTCAT GGAAGCGTCT ACAAGTCTGA GTTCATAGAC 
CTCTCAGAGA AGTTTAAGCA GGGAGAGGAT AACCTGGTGT TCGGGGTCAA GCCTGGTGAC 
CCTCGGGTGA TGCAGAAGCT GGACGATGAT GGGTTGCCAT TCATAGGCGC AAAGCTGGAG 
TATGGCGACC CTTACTACAG CTACCTCAAC CTTAACACTG GGGAAGGCTT CGTGGTTTAC 
TACAAGAGTA AAGAAAACTG TGTTGTGGAC AACATCAAAG TGTGTAGTAA CGACATGGGC 
AGTGGGAAGT TCAAGTGTAT CTGCATCACA GTCCGCATCC CCCGGAACCC AACCATTGGA 
GATAAGTTTG CCAGCCGACA TGGTCAGAAG GGCATTTTGA GCAGATTGTG GCCAGCCGAG 
GACATGCCTT TCACTGAGAG TGGCATGATG CCGGACATTC TGTTTAATCC TCACGGGTTT 
CCCTCCCGTA TGACCATAGG TATGTTAATC GAGAGCATGG CTGGGAAGTC TGCAGCTTTG 
CACGGTCTCT GCCATGATGC TACACCCTTC ATCTTCTCCG AGGAGAACTC TGCCTTAGAG 
TACTTTGGTG AGATGTTAAA GGCTGCTGGC TACAACTTCT ACGGCACCGA GAGACTGTAT 
AGTGGCATCA GTGGGATGGA GCTGGAGGCT GACATCTTCA TTGGTGTGGT TTATTACCAG 
CGCTTACGAC ACATGGTGTC AGACAAATTT CAAGTCAGAA CAACTGGAGC CAGGGACAAA 
GTCACCAACC AGCCCCTCGG GGGACGAAAT GTTCAGGGTG GAATCCGCTT TGGGGAGATG 
GAGCGGGATG CTCTGTTGGC ACACGGTACG TCTTTCCTTC TTCATGACCG GCTTTTCAAC 
TGCTCTGACC GTTCTGTCGC TCACATGTGT GTAGAGTGTG GCAGTTTGCT TTCTCCGCTA 
CTTGAGAAGC CTCCTCCGTC TTGGTCTGCG ATGCGCAACA GAAAGTACAA CTGCACTGTC 
TGTGGCCGCA GTGACACCAT CGACACCGTC TCTGTGCCGT ATGTCTTCCG GTACTTTGTA 
GCTGAGCTGG CAGCCATGAA CATCAAAGTG AAACTGGATG TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033112.2
CDS	92..3499
Translation	MDVDGRWRNLPSGPSLKHLTDPSYGIPPEQQKAALQDLTRAHVDSFNYAALEGLSHAVQAIPPFEFAFKDERISLTIVDAVISPPSVPKGTICKDLNVYPAECRGRKSTYRGRLTADISWAVNGVPKGIIKQFLGYVPIMVKSKLCNLYNLPPRVLIEHHEEAEEMGGYFIINGIEKVIRMLIVPRRNFPIAMVRPKWKSRGLGYTQFGVSMRCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFRNSVSQMLRIVIEEGCHSQKQVLNYLGECFRVKLSLPDWYPNVEAAEFLLNQCICIHLQSNTDKFYLLCLMTRKLFALARGECMDDNPDSLVNQEVLSPGQLFLMFLKEKMENWLVSIKIVLDKRAQKANVSINNENLMKIFSMGTELTRPFEYLLATGNLRSKTGLGFLQDSGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLLPESWGFLCPVHTPDGAPCGLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDTAPCRPYSDCYPVLLDGVMVGWVDKDLAPEVADTLRRFKVLREKRIPPWMEVALIPMTGKPSLYPGLFLFTTPCRLVRPVQNLELGREELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGEDNLVFGVKPGDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVYYKSKENCVVDNIKVCSNDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVI

Target ORF information:

RefSeq Version	NM_009086.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009086.2

ORF Insert Sequence:

ATGGATGTCG ACGGCCGGTG GCGGAACCTG CCCAGCGGGC CCAGCCTAAA GCATTTAACC 
GACCCCTCGT ACGGGATTCC CCCAGAGCAG CAAAAGGCAG CTTTGCAGGA CCTGACGCGG 
GCGCACGTGG ACTCCTTCAA CTACGCGGCG CTGGAGGGGC TGAGCCACGC GGTGCAGGCC 
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGCATAT CTCTTACCAT TGTGGATGCT 
GTCATCAGTC CACCCTCAGT GCCCAAAGGG ACCATCTGCA AAGATCTCAA CGTTTATCCA 
GCTGAGTGTC GTGGCCGAAA GAGCACCTAC CGAGGGAGGC TGACGGCTGA TATCAGCTGG 
GCCGTGAATG GAGTCCCCAA AGGGATCATT AAACAGTTTC TCGGCTATGT TCCCATCATG 
GTGAAGTCCA AGCTTTGCAA CTTATACAAC CTTCCTCCTC GAGTCCTCAT TGAGCACCAT 
GAGGAGGCAG AGGAAATGGG CGGTTATTTT ATAATCAATG GCATAGAAAA AGTCATCCGC 
ATGTTGATTG TGCCCCGGAG AAATTTCCCC ATCGCCATGG TAAGGCCGAA ATGGAAAAGC 
AGAGGACTCG GCTACACTCA GTTTGGGGTC TCCATGCGCT GTGTGAGAGA AGAGCACTCC 
GCCGTGAACA TGAACCTGCA CTATGTGGAG AACGGCACGG TTATGTTAAA CTTTATTTAC 
CGCAAAGAGC TCTTTTTCCT TCCTTTGGGA TTTGCACTTA AGGCACTCGT GAGCTTTTCT 
GATTATCAGA TTTTCCAGGA GCTTATCAAG GGCAAAGAGG AGGACTCTTT CTTTAGGAAT 
TCTGTTTCTC AGATGCTGCG GATTGTCATA GAAGAGGGCT GTCACTCACA GAAGCAGGTT 
CTTAACTATC TGGGAGAATG CTTCCGAGTG AAGCTCAGCC TTCCTGATTG GTACCCTAAC 
GTGGAAGCTG CCGAGTTCCT GCTTAACCAG TGCATCTGCA TCCACCTGCA GTCCAACACT 
GACAAGTTCT ACCTGCTCTG CCTCATGACC CGGAAGCTGT TCGCTTTAGC CAGAGGGGAG 
TGCATGGACG ACAACCCTGA TAGCCTAGTG AACCAAGAGG TCCTCAGCCC TGGGCAGCTC 
TTCCTGATGT TTCTGAAGGA AAAAATGGAA AACTGGCTAG TGTCTATTAA AATAGTTTTA 
GATAAGAGGG CTCAGAAGGC CAATGTTTCC ATAAACAACG AAAATTTGAT GAAGATTTTT 
AGTATGGGAA CAGAGCTAAC AAGACCATTT GAATATCTTC TTGCTACTGG GAATCTGCGT 
TCTAAAACAG GTCTCGGCTT CCTGCAAGAT TCTGGCCTGT GTGTTGTGGC TGACAAGCTG 
AACTTCCTTC GCTACCTCTC CCATTTCCGC TGTGTGCACA GAGGGGCTGC CTTTGCCAAG 
ATGAGGACCA CCACCGTGCG CAGGCTGCTG CCAGAATCCT GGGGCTTCCT CTGCCCTGTG 
CACACTCCAG ACGGGGCGCC TTGTGGGCTG TTGAACCACT TGACTGCCGT GTGTGAGGTT 
GTTACCAAGT TTGTGTACAC GGCATCTATT CCAGCCTTGC TCTGTGGCTT AGGAGTCACT 
CCTGTTGATA CAGCGCCATG TCGACCGTAC AGTGACTGCT ACCCTGTCCT GCTGGATGGC 
GTCATGGTGG GCTGGGTGGA TAAGGACCTT GCTCCTGAAG TTGCAGACAC TCTCCGTCGA 
TTCAAGGTGT TGAGAGAAAA AAGAATTCCT CCCTGGATGG AGGTGGCCCT GATTCCCATG 
ACAGGAAAAC CAAGTCTGTA CCCAGGTCTG TTCCTCTTTA CCACTCCCTG CAGGCTGGTG 
AGGCCTGTGC AGAACTTGGA ACTGGGCAGA GAAGAGCTCA TTGGAACCAT GGAACAGCTC 
TTCATGAACG TCGCCATCTT TGAGGACGAG GTGTTTGGTG GCATCTCCAC ACACCAGGAG 
CTCTTCCCTC ACAGCCTGCT GAGCGTGATC GCCAACTTCA TCCCCTTCTC GGATCACAAC 
CAGAGTCCTC GGAACATGTA CCAGTGCCAG ATGGGTAAGC AGACCATGGG CTTTCCGCTG 
CTCACTTACC AAAACCGATC AGATAATAAA CTCTATCGAC TCCAAACCCC TCAGAGCCCT 
CTAGTGAGAC CGTGCATGTA TGATTTCTAT GACATGGACA ACTATCCGAT CGGGACAAAC 
GCCATTGTAG CTGTGATCTC CTACACTGGC TATGATATGG AGGACGCCAT GATTGTCAAC 
AAGGCTTCCT GGGAACGAGG CTTCGCTCAT GGAAGCGTCT ACAAGTCTGA GTTCATAGAC 
CTCTCAGAGA AGTTTAAGCA GGGAGAGGAT AACCTGGTGT TCGGGGTCAA GCCTGGTGAC 
CCTCGGGTGA TGCAGAAGCT GGACGATGAT GGGTTGCCAT TCATAGGCGC AAAGCTGGAG 
TATGGCGACC CTTACTACAG CTACCTCAAC CTTAACACTG GGGAAGGCTT CGTGGTTTAC 
TACAAGAGTA AAGAAAACTG TGTTGTGGAC AACATCAAAG TGTGTAGTAA CGACATGGGC 
AGTGGGAAGT TCAAGTGTAT CTGCATCACA GTCCGCATCC CCCGGAACCC AACCATTGGA 
GATAAGTTTG CCAGCCGACA TGGTCAGAAG GGCATTTTGA GCAGATTGTG GCCAGCCGAG 
GACATGCCTT TCACTGAGAG TGGCATGATG CCGGACATTC TGTTTAATCC TCACGGGTTT 
CCCTCCCGTA TGACCATAGG TATGTTAATC GAGAGCATGG CTGGGAAGTC TGCAGCTTTG 
CACGGTCTCT GCCATGATGC TACACCCTTC ATCTTCTCCG AGGAGAACTC TGCCTTAGAG 
TACTTTGGTG AGATGTTAAA GGCTGCTGGC TACAACTTCT ACGGCACCGA GAGACTGTAT 
AGTGGCATCA GTGGGATGGA GCTGGAGGCT GACATCTTCA TTGGTGTGGT TTATTACCAG 
CGCTTACGAC ACATGGTGTC AGACAAATTT CAAGTCAGAA CAACTGGAGC CAGGGACAAA 
GTCACCAACC AGCCCCTCGG GGGACGAAAT GTTCAGGGTG GAATCCGCTT TGGGGAGATG 
GAGCGGGATG CTCTGTTGGC ACACGGTACG TCTTTCCTTC TTCATGACCG GCTTTTCAAC 
TGCTCTGACC GTTCTGTCGC TCACATGTGT GTAGAGTGTG GCAGTTTGCT TTCTCCGCTA 
CTTGAGAAGC CTCCTCCGTC TTGGTCTGCG ATGCGCAACA GAAAGTACAA CTGCACTGTC 
TGTGGCCGCA GTGACACCAT CGACACCGTC TCTGTGCCGT ATGTCTTCCG GTACTTTGTA 
GCTGAGCTGG CAGCCATGAA CATCAAAGTG AAACTGGATG TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr1b ( XM_006499025.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499088.1 Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X1, mRNA.

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CloneID	OMu30175
Clone ID Related Accession (Same CDS sequence)	XM_006499025.4 , XM_006499025.3
Accession Version	XM_006499025.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCG ACGGCCGGTG GCGGAACCTG CCCAGCGGGC CCAGCCTAAA GCATTTAACC 
GACCCCTCGT ACGGGATTCC CCCAGAGCAG CAAAAGGCAG CTTTGCAGGA CCTGACGCGG 
GCGCACGTGG ACTCCTTCAA CTACGCGGCG CTGGAGGGGC TGAGCCACGC GGTGCAGGCC 
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGCATAT CTCTTACCAT TGTGGATGCT 
GTCATCAGTC CACCCTCAGT GCCCAAAGGG ACCATCTGCA AAGATCTCAA CGTTTATCCA 
GCTGAGTGTC GTGGCCGAAA GAGCACCTAC CGAGGGAGGC TGACGGCTGA TATCAGCTGG 
GCCGTGAATG GAGTCCCCAA AGGGATCATT AAACAGTTTC TCGGCTATGT TCCCATCATG 
GTGAAGTCCA AGCTTTGCAA CTTATACAAC CTTCCTCCTC GAGTCCTCAT TGAGCACCAT 
GAGGAGGCAG AGGAAATGGG CGGTTATTTT ATAATCAATG GCATAGAAAA AGTCATCCGC 
ATGTTGATTG TGCCCCGGAG AAATTTCCCC ATCGCCATGG TAAGGCCGAA ATGGAAAAGC 
AGAGGACTCG GCTACACTCA GTTTGGGGTC TCCATGCGCT GTGTGAGAGA AGAGCACTCC 
GCCGTGAACA TGAACCTGCA CTATGTGGAG AACGGCACGG TTATGTTAAA CTTTATTTAC 
CGCAAAGAGC TCTTTTTCCT TCCTTTGGGA TTTGCACTTA AGGCACTCGT GAGCTTTTCT 
GATTATCAGA TTTTCCAGGA GCTTATCAAG GGCAAAGAGG AGGACTCTTT CTTTAGGAAT 
TCTGTTTCTC AGATGCTGCG GATTGTCATA GAAGAGGGCT GTCACTCACA GAAGCAGGTT 
CTTAACTATC TGGGAGAATG CTTCCGAGTG AAGCTCAGCC TTCCTGATTG GTACCCTAAC 
GTGGAAGCTG CCGAGTTCCT GCTTAACCAG TGCATCTGCA TCCACCTGCA GTCCAACACT 
GACAAGTTCT ACCTGCTCTG CCTCATGACC CGGAAGCTGT TCGCTTTAGC CAGAGGGGAG 
TGCATGGACG ACAACCCTGA TAGCCTAGTG AACCAAGAGG TCCTCAGCCC TGGGCAGCTC 
TTCCTGATGT TTCTGAAGGA AAAAATGGAA AACTGGCTAG TGTCTATTAA AATAGTTTTA 
GATAAGAGGG CTCAGAAGGC CAATGTTTCC ATAAACAACG AAAATTTGAT GAAGATTTTT 
AGTATGGGAA CAGAGCTAAC AAGACCATTT GAATATCTTC TTGCTACTGG GAATCTGCGT 
TCTAAAACAG GTCTCGGCTT CCTGCAAGAT TCTGGCCTGT GTGTTGTGGC TGACAAGCTG 
AACTTCCTTC GCTACCTCTC CCATTTCCGC TGTGTGCACA GAGGGGCTGC CTTTGCCAAG 
ATGAGGACCA CCACCGTGCG CAGGCTGCTG CCAGAATCCT GGGGCTTCCT CTGCCCTGTG 
CACACTCCAG ACGGGGCGCC TTGTGGGCTG TTGAACCACT TGACTGCCGT GTGTGAGGAG 
TCACTCCTGT TGATACAGCG CCATGTCGAC CGTACAGTGA CTGCTACCCT GTCCTGCTGG 
ATGGCGTCAT GGTGGGCTGG GTGGATAAGG ACCTTGCTCC TGAAGTTGCA GACACTCTCC 
GTCGATTCAA GGCTGGTGAG GCCTGTGCAG AACTTGGAAC TGGGCAGAGA AGAGCTCATT 
GGAACCATGG AACAGCTCTT CATGAACGTC GCCATCTTTG AGGACGAGGT GTTTGGTGGC 
ATCTCCACAC ACCAGGAGCT CTTCCCTCAC AGCCTGCTGA GCGTGATCGC CAACTTCATC 
CCCTTCTCGG ATCACAACCA GAGTCCTCGG AACATGTACC AGTGCCAGAT GGGTAAGCAG 
ACCATGGGCT TTCCGCTGCT CACTTACCAA AACCGATCAG ATAATAAACT CTATCGACTC 
CAAACCCCTC AGAGCCCTCT AGTGAGACCG TGCATGTATG ATTTCTATGA CATGGACAAC 
TATCCGATCG GGACAAACGC CATTGTAGCT GTGATCTCCT ACACTGGCTA TGATATGGAG 
GACGCCATGA TTGTCAACAA GGCTTCCTGG GAACGAGGCT TCGCTCATGG AAGCGTCTAC 
AAGTCTGAGT TCATAGACCT CTCAGAGAAG TTTAAGCAGG GAGAGGATAA CCTGGTGTTC 
GGGGTCAAGC CTGGTGACCC TCGGGTGATG CAGAAGCTGG ACGATGATGG GTTGCCATTC 
ATAGGCGCAA AGCTGGAGTA TGGCGACCCT TACTACAGCT ACCTCAACCT TAACACTGGG 
GAAGGCTTCG TGGTTTACTA CAAGAGTAAA GAAAACTGTG TTGTGGACAA CATCAAAGTG 
TGTAGTAACG ACATGGGCAG TGGGAAGTTC AAGTGTATCT GCATCACAGT CCGCATCCCC 
CGGAACCCAA CCATTGGAGA TAAGTTTGCC AGCCGACATG GTCAGAAGGG CATTTTGAGC 
AGATTGTGGC CAGCCGAGGA CATGCCTTTC ACTGAGAGTG GCATGATGCC GGACATTCTG 
TTTAATCCTC ACGGGTTTCC CTCCCGTATG ACCATAGGTA TGTTAATCGA GAGCATGGCT 
GGGAAGTCTG CAGCTTTGCA CGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCCGAG 
GAGAACTCTG CCTTAGAGTA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAACTTCTAC 
GGCACCGAGA GACTGTATAG TGGCATCAGT GGGATGGAGC TGGAGGCTGA CATCTTCATT 
GGTGTGGTTT ATTACCAGCG CTTACGACAC ATGGTGTCAG ACAAATTTCA AGTCAGAACA 
ACTGGAGCCA GGGACAAAGT CACCAACCAG CCCCTCGGGG GACGAAATGT TCAGGGTGGA 
ATCCGCTTTG GGGAGATGGA GCGGGATGCT CTGTTGGCAC ACGGTACGTC TTTCCTTCTT 
CATGACCGGC TTTTCAACTG CTCTGACCGT TCTGTCGCTC ACATGTGTGT AGAGTGTGGC 
AGTTTGCTTT CTCCGCTACT TGAGAAGCCT CCTCCGTCTT GGTCTGCGAT GCGCAACAGA 
AAGTACAACT GCACTGTCTG TGGCCGCAGT GACACCATCG ACACCGTCTC TGTGCCGTAT 
GTCTTCCGGT ACTTTGTAGC TGAGCTGGCA GCCATGAACA TCAAAGTGAA ACTGGATGTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499088.1
CDS	93..3338
Translation	MDVDGRWRNLPSGPSLKHLTDPSYGIPPEQQKAALQDLTRAHVDSFNYAALEGLSHAVQAIPPFEFAFKDERISLTIVDAVISPPSVPKGTICKDLNVYPAECRGRKSTYRGRLTADISWAVNGVPKGIIKQFLGYVPIMVKSKLCNLYNLPPRVLIEHHEEAEEMGGYFIINGIEKVIRMLIVPRRNFPIAMVRPKWKSRGLGYTQFGVSMRCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFRNSVSQMLRIVIEEGCHSQKQVLNYLGECFRVKLSLPDWYPNVEAAEFLLNQCICIHLQSNTDKFYLLCLMTRKLFALARGECMDDNPDSLVNQEVLSPGQLFLMFLKEKMENWLVSIKIVLDKRAQKANVSINNENLMKIFSMGTELTRPFEYLLATGNLRSKTGLGFLQDSGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLLPESWGFLCPVHTPDGAPCGLLNHLTAVCEESLLLIQRHVDRTVTATLSCWMASWWAGWIRTLLLKLQTLSVDSRLVRPVQNLELGREELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKFKQGEDNLVFGVKPGDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVYYKSKENCVVDNIKVCSNDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMMPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVI

Target ORF information:

RefSeq Version	XM_006499025.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) I polypeptide B (Polr1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499025.3

ORF Insert Sequence:

ATGGATGTCG ACGGCCGGTG GCGGAACCTG CCCAGCGGGC CCAGCCTAAA GCATTTAACC 
GACCCCTCGT ACGGGATTCC CCCAGAGCAG CAAAAGGCAG CTTTGCAGGA CCTGACGCGG 
GCGCACGTGG ACTCCTTCAA CTACGCGGCG CTGGAGGGGC TGAGCCACGC GGTGCAGGCC 
ATACCTCCCT TTGAATTTGC TTTCAAAGAT GAGCGCATAT CTCTTACCAT TGTGGATGCT 
GTCATCAGTC CACCCTCAGT GCCCAAAGGG ACCATCTGCA AAGATCTCAA CGTTTATCCA 
GCTGAGTGTC GTGGCCGAAA GAGCACCTAC CGAGGGAGGC TGACGGCTGA TATCAGCTGG 
GCCGTGAATG GAGTCCCCAA AGGGATCATT AAACAGTTTC TCGGCTATGT TCCCATCATG 
GTGAAGTCCA AGCTTTGCAA CTTATACAAC CTTCCTCCTC GAGTCCTCAT TGAGCACCAT 
GAGGAGGCAG AGGAAATGGG CGGTTATTTT ATAATCAATG GCATAGAAAA AGTCATCCGC 
ATGTTGATTG TGCCCCGGAG AAATTTCCCC ATCGCCATGG TAAGGCCGAA ATGGAAAAGC 
AGAGGACTCG GCTACACTCA GTTTGGGGTC TCCATGCGCT GTGTGAGAGA AGAGCACTCC 
GCCGTGAACA TGAACCTGCA CTATGTGGAG AACGGCACGG TTATGTTAAA CTTTATTTAC 
CGCAAAGAGC TCTTTTTCCT TCCTTTGGGA TTTGCACTTA AGGCACTCGT GAGCTTTTCT 
GATTATCAGA TTTTCCAGGA GCTTATCAAG GGCAAAGAGG AGGACTCTTT CTTTAGGAAT 
TCTGTTTCTC AGATGCTGCG GATTGTCATA GAAGAGGGCT GTCACTCACA GAAGCAGGTT 
CTTAACTATC TGGGAGAATG CTTCCGAGTG AAGCTCAGCC TTCCTGATTG GTACCCTAAC 
GTGGAAGCTG CCGAGTTCCT GCTTAACCAG TGCATCTGCA TCCACCTGCA GTCCAACACT 
GACAAGTTCT ACCTGCTCTG CCTCATGACC CGGAAGCTGT TCGCTTTAGC CAGAGGGGAG 
TGCATGGACG ACAACCCTGA TAGCCTAGTG AACCAAGAGG TCCTCAGCCC TGGGCAGCTC 
TTCCTGATGT TTCTGAAGGA AAAAATGGAA AACTGGCTAG TGTCTATTAA AATAGTTTTA 
GATAAGAGGG CTCAGAAGGC CAATGTTTCC ATAAACAACG AAAATTTGAT GAAGATTTTT 
AGTATGGGAA CAGAGCTAAC AAGACCATTT GAATATCTTC TTGCTACTGG GAATCTGCGT 
TCTAAAACAG GTCTCGGCTT CCTGCAAGAT TCTGGCCTGT GTGTTGTGGC TGACAAGCTG 
AACTTCCTTC GCTACCTCTC CCATTTCCGC TGTGTGCACA GAGGGGCTGC CTTTGCCAAG 
ATGAGGACCA CCACCGTGCG CAGGCTGCTG CCAGAATCCT GGGGCTTCCT CTGCCCTGTG 
CACACTCCAG ACGGGGCGCC TTGTGGGCTG TTGAACCACT TGACTGCCGT GTGTGAGGAG 
TCACTCCTGT TGATACAGCG CCATGTCGAC CGTACAGTGA CTGCTACCCT GTCCTGCTGG 
ATGGCGTCAT GGTGGGCTGG GTGGATAAGG ACCTTGCTCC TGAAGTTGCA GACACTCTCC 
GTCGATTCAA GGCTGGTGAG GCCTGTGCAG AACTTGGAAC TGGGCAGAGA AGAGCTCATT 
GGAACCATGG AACAGCTCTT CATGAACGTC GCCATCTTTG AGGACGAGGT GTTTGGTGGC 
ATCTCCACAC ACCAGGAGCT CTTCCCTCAC AGCCTGCTGA GCGTGATCGC CAACTTCATC 
CCCTTCTCGG ATCACAACCA GAGTCCTCGG AACATGTACC AGTGCCAGAT GGGTAAGCAG 
ACCATGGGCT TTCCGCTGCT CACTTACCAA AACCGATCAG ATAATAAACT CTATCGACTC 
CAAACCCCTC AGAGCCCTCT AGTGAGACCG TGCATGTATG ATTTCTATGA CATGGACAAC 
TATCCGATCG GGACAAACGC CATTGTAGCT GTGATCTCCT ACACTGGCTA TGATATGGAG 
GACGCCATGA TTGTCAACAA GGCTTCCTGG GAACGAGGCT TCGCTCATGG AAGCGTCTAC 
AAGTCTGAGT TCATAGACCT CTCAGAGAAG TTTAAGCAGG GAGAGGATAA CCTGGTGTTC 
GGGGTCAAGC CTGGTGACCC TCGGGTGATG CAGAAGCTGG ACGATGATGG GTTGCCATTC 
ATAGGCGCAA AGCTGGAGTA TGGCGACCCT TACTACAGCT ACCTCAACCT TAACACTGGG 
GAAGGCTTCG TGGTTTACTA CAAGAGTAAA GAAAACTGTG TTGTGGACAA CATCAAAGTG 
TGTAGTAACG ACATGGGCAG TGGGAAGTTC AAGTGTATCT GCATCACAGT CCGCATCCCC 
CGGAACCCAA CCATTGGAGA TAAGTTTGCC AGCCGACATG GTCAGAAGGG CATTTTGAGC 
AGATTGTGGC CAGCCGAGGA CATGCCTTTC ACTGAGAGTG GCATGATGCC GGACATTCTG 
TTTAATCCTC ACGGGTTTCC CTCCCGTATG ACCATAGGTA TGTTAATCGA GAGCATGGCT 
GGGAAGTCTG CAGCTTTGCA CGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCCGAG 
GAGAACTCTG CCTTAGAGTA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAACTTCTAC 
GGCACCGAGA GACTGTATAG TGGCATCAGT GGGATGGAGC TGGAGGCTGA CATCTTCATT 
GGTGTGGTTT ATTACCAGCG CTTACGACAC ATGGTGTCAG ACAAATTTCA AGTCAGAACA 
ACTGGAGCCA GGGACAAAGT CACCAACCAG CCCCTCGGGG GACGAAATGT TCAGGGTGGA 
ATCCGCTTTG GGGAGATGGA GCGGGATGCT CTGTTGGCAC ACGGTACGTC TTTCCTTCTT 
CATGACCGGC TTTTCAACTG CTCTGACCGT TCTGTCGCTC ACATGTGTGT AGAGTGTGGC 
AGTTTGCTTT CTCCGCTACT TGAGAAGCCT CCTCCGTCTT GGTCTGCGAT GCGCAACAGA 
AAGTACAACT GCACTGTCTG TGGCCGCAGT GACACCATCG ACACCGTCTC TGTGCCGTAT 
GTCTTCCGGT ACTTTGTAGC TGAGCTGGCA GCCATGAACA TCAAAGTGAA ACTGGATGTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W

Mechanical loading induces the expression of a Pol I regulon at the onset of skeletal muscle hypertrophy.
American journal of physiology. Cell physiology302(10)C1523-30(2012 May)
von Walden F,Casagrande V,?stlund Farrants AK,Nader GA

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene.
Biochemical and biophysical research communications365(4)636-42(2008 Jan)
Chen H,Li Z,Haruna K,Li Z,Li Z,Semba K,Araki M,Yamamura K,Araki K

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
BMC developmental biology514(2005 Jul)
McKee AE,Minet E,Stern C,Riahi S,Stiles CD,Silver PA

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Polr1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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