Rit2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rit2
Entrez Gene ID	19762
Full Name	Ras-like without CAAX 2
Synonyms	RIBA,Rin,Roc2
General protein information	Preferred Names Ras-like without CAAX 2 Names GTP-binding protein Rit2 GTP-binding protein Roc2 RAS-like protein expressed in neuron ras-like protein expressed in neurons ras-like without CAAX protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_009065.2	NP_033091.1	GTP-binding protein Rit2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rit2	NP_001013078.1
Homo sapiens (human)	RIT2	NP_002921.1
Canis lupus familiaris (dog)	RIT2	XP_547597.3
Mus musculus (house mouse)	Rit2	NP_033091.1
Gallus gallus (chicken)	RIT2	XP_001233997.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005159	XP_314049.4
	RIT2	XP_001083497.1
Drosophila melanogaster (fruit fly)	Ric	NP_001163165.1

Gene Ontology
Bibliography

Related articles in PubMed

RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors.
Zhang L, Wahlin K, Li Y, Masuda T, Yang Z, Zack DJ, Esumi N
Molecular vision191371-86(2013)

Pituitary adenylate cyclase-activating polypeptide 38-mediated Rin activation requires Src and contributes to the regulation of HSP27 signaling during neuronal differentiation.
Shi GX, Jin L, Andres DA
Molecular and cellular biology28(16)4940-51(2008 Aug)

Plexin B3 promotes neurite outgrowth, interacts homophilically, and interacts with Rin.
Hartwig C, Veske A, Krejcova S, Rosenberger G, Finckh U
BMC neuroscience653(2005 Aug)

Small GTPase proteins Rin and Rit Bind to PAR6 GTP-dependently and regulate cell transformation.
Hoshino M, Yoshimori T, Nakamura S
The Journal of biological chemistry280(24)22868-74(2005 Jun)

Cell-free cotranslation and selection using in vitro virus for high-throughput analysis of protein-protein interactions and complexes.
Miyamoto-Sato E, Ishizaka M, Horisawa K, Tateyama S, Takashima H, Fuse S, Sue K, Hirai N, Masuoka K, Yanagawa H
Genome research15(5)710-7(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies confirm the expression of RIT2 in retinal neuronal cells.
Title: RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors.

Neuron-specific small GTPase Rin is involved in downstream signaling of plexin B3.
Title: Plexin B3 promotes neurite outgrowth, interacts homophilically, and interacts with Rin.

Rin and Rit Bind to PAR6 GTP-dependently and regulate cell transformation
Title: Small GTPase proteins Rin and Rit Bind to PAR6 GTP-dependently and regulate cell transformation.

The Ras-like small GTP-binding protein Rin is activated by growth factor stimulation
Title: The Ras-like small GTP-binding protein Rin is activated by growth factor stimulation.

stimulation of PC6 cells with either epidermal growth factor or nerve growth factor (NGF) results in rapid activation of Rin
Title: Nerve growth factor-dependent activation of the small GTPase Rin.

The following Rit2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rit2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03448	NM_009065.2 Latest version!	Mus musculus Ras-like without CAAX 2 (Rit2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rit2 ( NM_009065.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033091.1 Mus musculus Ras-like without CAAX 2 (Rit2), mRNA.

Price & Availability↑

CloneID	OMu03448
Clone ID Related Accession (Same CDS sequence)	NM_009065.2
Accession Version	NM_009065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	GTP-binding protein Rit2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC018267.1. On or before Aug 30, 2004 this sequence version replaced XM_356992.1, NM_009065.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018267.1, AK140726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGTAG AAAACGAAGC CCACTGCTGC CCTGGCAGCT CATCAGGCGG GTCCAGAGAG 
TACAAGGTGG TAATGCTGGG CGCAGGGGGC GTTGGTAAAA GCGCAGTCAC AATGCAGTTT 
ATAAGCCACC AGTTCCCGGA CTATCACGAC CCCACAATCG AAGATGCTTA TAAAACCCAG 
GTGAGGATTG ATAATGAGCC TGCTTACTTA GACATCTTGG ACACTGCTGG TCAGGCAGAG 
TTCACGGCCA TGCGGGAGCA GTACATGCGT GGGGGAGAGG GCTTCATCAT CTGCTATTCT 
GTCACTGACC GCCAGTCATT CCAGGAGGCT GCCAAGTTCA AGGAGCTTAT TTTCCAGGTC 
CGTCACACCT ATGAAATTCC CCTTGTGCTA GTGGGTAACA AAATTGACTT GGAGCAGTTC 
CGTCAGGTAT CTACAGAAGA AGGCATGAAT CTTGCTCGAG ACTACAACTG TGCCTTCTTT 
GAGACATCTG CAGCCCTGCG ATTCGGTATC GATGATGCTT TTCAAGGCTT AGTGAGAGAA 
ATTCGCAGGA AGGAATCCAT GCTGTCCTTG GTGGAAAGGA AATTGAAGAG GAAGGACAGC 
CTGTGGAAGA AGATAAAAGC CTCCCTGAAG AAGAAGAGAG AAAACATGTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033091.1
CDS	186..839
Translation	MEVENEAHCCPGSSSGGSREYKVVMLGAGGVGKSAVTMQFISHQFPDYHDPTIEDAYKTQVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIPLVLVGNKIDLEQFRQVSTEEGMNLARDYNCAFFETSAALRFGIDDAFQGLVREIRRKESMLSLVERKLKRKDSLWKKIKASLKKKRENML

Target ORF information:

RefSeq Version	NM_009065.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ras-like without CAAX 2 (Rit2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009065.2

ORF Insert Sequence:

ATGGAAGTAG AAAACGAAGC CCACTGCTGC CCTGGCAGCT CATCAGGCGG GTCCAGAGAG 
TACAAGGTGG TAATGCTGGG CGCAGGGGGC GTTGGTAAAA GCGCAGTCAC AATGCAGTTT 
ATAAGCCACC AGTTCCCGGA CTATCACGAC CCCACAATCG AAGATGCTTA TAAAACCCAG 
GTGAGGATTG ATAATGAGCC TGCTTACTTA GACATCTTGG ACACTGCTGG TCAGGCAGAG 
TTCACGGCCA TGCGGGAGCA GTACATGCGT GGGGGAGAGG GCTTCATCAT CTGCTATTCT 
GTCACTGACC GCCAGTCATT CCAGGAGGCT GCCAAGTTCA AGGAGCTTAT TTTCCAGGTC 
CGTCACACCT ATGAAATTCC CCTTGTGCTA GTGGGTAACA AAATTGACTT GGAGCAGTTC 
CGTCAGGTAT CTACAGAAGA AGGCATGAAT CTTGCTCGAG ACTACAACTG TGCCTTCTTT 
GAGACATCTG CAGCCCTGCG ATTCGGTATC GATGATGCTT TTCAAGGCTT AGTGAGAGAA 
ATTCGCAGGA AGGAATCCAT GCTGTCCTTG GTGGAAAGGA AATTGAAGAG GAAGGACAGC 
CTGTGGAAGA AGATAAAAGC CTCCCTGAAG AAGAAGAGAG AAAACATGTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors.
Molecular vision191371-86(2013)
Zhang L,Wahlin K,Li Y,Masuda T,Yang Z,Zack DJ,Esumi N

Pituitary adenylate cyclase-activating polypeptide 38-mediated Rin activation requires Src and contributes to the regulation of HSP27 signaling during neuronal differentiation.
Molecular and cellular biology28(16)4940-51(2008 Aug)
Shi GX,Jin L,Andres DA

Plexin B3 promotes neurite outgrowth, interacts homophilically, and interacts with Rin.
BMC neuroscience653(2005 Aug)
Hartwig C,Veske A,Krejcova S,Rosenberger G,Finckh U

Small GTPase proteins Rin and Rit Bind to PAR6 GTP-dependently and regulate cell transformation.
The Journal of biological chemistry280(24)22868-74(2005 Jun)
Hoshino M,Yoshimori T,Nakamura S

Cell-free cotranslation and selection using in vitro virus for high-throughput analysis of protein-protein interactions and complexes.
Genome research15(5)710-7(2005 May)
Miyamoto-Sato E,Ishizaka M,Horisawa K,Tateyama S,Takashima H,Fuse S,Sue K,Hirai N,Masuoka K,Yanagawa H