Rasgrf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasgrf1
Entrez Gene ID	19417
Full Name	RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms	AI844718,CDC25,CDC25Mm,Gnrp,Grf1,Grfbeta,P190-A,Ras-GRF1,p190,p190RhoGEF
General protein information	Preferred Names RAS protein-specific guanine nucleotide-releasing factor 1 Names ras-specific guanine nucleotide-releasing factor 1 Ras guanine release factor beta ras-specific nucleotide exchange factor CDC25
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 E3.1\|9 47.31 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357744.1	NP_001344673.1	ras-specific guanine nucleotide-releasing factor 1 isoform 4
NM_001357743.1	NP_001344672.1	ras-specific guanine nucleotide-releasing factor 1 isoform 3
NM_001039655.1	NP_001034744.1	ras-specific guanine nucleotide-releasing factor 1 isoform 2
XM_006510875.3	XP_006510938.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
NM_011245.2	NP_035375.1	ras-specific guanine nucleotide-releasing factor 1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGRF1	NP_002882.3
Mus musculus (house mouse)	Rasgrf1	NP_035375.1
Danio rerio (zebrafish)	si:ch211-234p18.3	XP_002665395.1
Xenopus tropicalis (tropical clawed frog)	rasgrf1	XP_002933009.2
Gallus gallus (chicken)	RASGRF1	XP_004944008.1
Pan troglodytes (chimpanzee)	RASGRF1	XP_001153586.1
Canis lupus familiaris (dog)	RASGRF1	XP_545892.5
Rattus norvegicus (Norway rat)	Rasgrf1	NP_001164002.1
Bos taurus (cattle)	RASGRF1	NP_001178386.1

Gene Ontology

The following Rasgrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasgrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18654	NM_001039655.1 Latest version!	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu83773	NM_001357744.1 Latest version!	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu18799	NM_011245.2 Latest version!	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$741.30 $1059.00
OMu42600	NM_001357743.1 Latest version!	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu42600	XM_006510875.3 Latest version!	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasgrf1 ( NM_001039655.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034744.1 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18654
Clone ID Related Accession (Same CDS sequence)	NM_001039655.1
Accession Version	NM_001039655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102545.9 and AK140227.1. Transcript Variant: This variant (2) lacks multiple 3' coding exons and uses an alternate 3' terminal exon, compared to variant 1. The resulting protein (isoform 2, also known as GRF beta) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK140227.1, AK162908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCGTC ATAGCAGGAG TCGACGTCGA 
CGTAATGTTC TGAGAAGACA ACCAACCAGG ATCTCAAAAA CATTCAGGCT GGTGGCAATT 
GAATTGACTG GGATGACAGC CACCCTTGAA GTCTCCTTCT CCATAATTAG GACAGAGATC 
GGTACCGTTG CTCCAGCTCC ACAGACATGG ATGGGAAAGC CAGAAGACTT GACAACGCTG 
CGTGACAGAG TGGAGTCGAA GCGTCTCTTC CACCATCCCT GTAGATTGAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034744.1
CDS	224..760
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQRHSRSRRRRNVLRRQPTRISKTFRLVAIELTGMTATLEVSFSIIRTEIGTVAPAPQTWMGKPEDLTTLRDRVESKRLFHHPCRLII

Target ORF information:

RefSeq Version	NM_001039655.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039655.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCGTC ATAGCAGGAG TCGACGTCGA 
CGTAATGTTC TGAGAAGACA ACCAACCAGG ATCTCAAAAA CATTCAGGCT GGTGGCAATT 
GAATTGACTG GGATGACAGC CACCCTTGAA GTCTCCTTCT CCATAATTAG GACAGAGATC 
GGTACCGTTG CTCCAGCTCC ACAGACATGG ATGGGAAAGC CAGAAGACTT GACAACGCTG 
CGTGACAGAG TGGAGTCGAA GCGTCTCTTC CACCATCCCT GTAGATTGAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( NM_001357744.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344673.1 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu83773
Clone ID Related Accession (Same CDS sequence)	NM_001357744.1
Accession Version	NM_001357744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102545.9 and DV043084.1. Transcript Variant: This variant (4) lacks multiple 3' coding exons and uses an alternate 3' terminal exon compared to variant 1. The resulting isoform (4) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DV043084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGGTAC CGTTGCTCCA GCTCCACAGA 
CATGGATGGG AAAGCCAGAA GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344673.1
CDS	224..550
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQVPLLQLHRHGWESQKT

Target ORF information:

RefSeq Version	NM_001357744.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357744.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGGTAC CGTTGCTCCA GCTCCACAGA 
CATGGATGGG AAAGCCAGAA GACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( NM_011245.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035375.1 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18799
Clone ID Related Accession (Same CDS sequence)	NM_011245.2
Accession Version	NM_011245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102545.9, AK140380.1, AK079638.1 and CD775453.1. On Mar 1, 2006 this sequence version replaced NM_011245.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138683.1, AK140380.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ATTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGTTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCTG CGATCGCTCG CGCCAGCTAC AAGATACTGG CTACAGAACA TGAGGCGCTT 
ATGCAGAAGT ACTTACACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAGCAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGGTAACT 
ATTACAAACC TGATCAAGGA CAATGATCGT ATCCAATCCA GCAACAAAGC CGGTTCTGCT 
GATGATGAGG ACAGTGACAT CAAGAAAATT AAGAAGGTTC AGAGCTTCCT TCGGGGATGG 
CTGTGCCGGC GAAAGTGGAA GAACATCATC CAGGACTACA TCCGGTCTCC CCACGCGGAC 
AGCATGCGCA AGAGGAACCA GGTGGTGTTC AGCATGCTGG AGGCCGAGGC TGAGTACGTC 
CAGCAGCTAC ACATCCTTGT CAACAACTTC CTGCGCCCAC TGCGCATGGC CGCCAGCTCT 
AAGAAACCCC CTATAACACA CGACGACGTC AGCAGTATCT TTCTGAACAG CGAGACCATC 
ATGTTCCTGC ATCAGATCTT CTACCAAGGC CTGAAGGCCC GTATCTCCAG CTGGCCCACC 
CTGGTGCTGG CGGACCTGTT CGATATCCTG CTGCCCATGC TGAACATCTA CCAGGAGTTC 
GTCCGCAACC ACCAGTACAG CCTCCAGATC CTTGCTCACT GCAAGCAAAA CCGGGACTTT 
GACAAGCTCC TCAAGCAGTA CGAGGCCAAG CCAGACTGCG AGGAGCGCAC ACTGGAGACC 
TTCCTCACCT ACCCAATGTT CCAGATCCCC AGGTACATCC TGACACTCCA TGAGCTGCTG 
GCCCACACAC CTCATGAGCA TGTGGAGCGC AACAGCCTGG ACTATGCCAA ATCCAAACTA 
GAGGAACTGT CCAGGATCAT GCATGACGAA GTCAGTGAAA CCGAGAACAT CCGCAAAAAC 
CTGGCCATTG AGCGTATGAT CACGGAGGGC TGTGAGATCC TCCTGGACAC CAGCCAGACC 
TTCGTGCGCC AAGGTTCCCT CATGCAGATG TCCTTGTCTG AAAAGAGCAA GTCCTCTAGG 
GGCCGCCTGG GGTCTCTGTC CACTAAGAAA GAGGGTGAGC GCCAGTGTTT CCTATTCTCC 
AAGCATCTCA TTATCTGCAC CAGAGGCTCT GGTGGGAAAC TGCACCTAAC CAAGAATGGA 
GTCATATCCC TCATTGACTG CACCTTACTG GATGAACCCG AAAACCTGGA CGACGAAGCC 
AAAGGAGCCG GACCAGAGAT AGAACACCTG GAGTTTAAGA TCGGGGTGGA GCCAAAGGAC 
TCCCTACCCT TCACTGTCAT CCTGGTAGCC TCAACCAGGC AGGAGAAGGC AGCATGGACC 
AGTGACATCA TCCAGTGCGT GGATAATATC CGCTGCAATG GGCTCATGAT GAATGCCTTT 
GAAGAAAATT CCAAGGTCAC CGTGCCTCAG ATGATCAAGT CTGACGCTTC CTTATATTGT 
GATGATGTTG ACATTCGCTT CAGCAAAACC ATGAATTCTT GCAAAGTGCT TCAGATCCGC 
TATGCCAGCG TGGAGCGCCT GCTGGAGCGC CTGACTGATC TTCGCTTCCT GAGCATTGAC 
TTTCTCAACA CCTTCCTGCA TTCCTATCGC GTCTTCACCA ATGCTATGGT GGTCCTGGAC 
AAGCTGATCA ATATCTACAG AAAACCCATG AGTGCGATTC CTGCCAGGTC CCTGGAACTC 
TTGTTTTCCA GCAGCCACAA CGCCAAGCTT CTGTACGGAG ATGCCCCTAA GTCCCCTCGC 
GCCAGCCGCA AGTTCTCCTC GCCACCGCCC TTGGCCATCG GGACGTCATC CCCCAGCCGC 
CGTCGGAAGC TGTCCCTCAA CATCCCCATC ATCACAGGCG GCAAGGCGCT GGAACTGGCT 
TCCCTTGGGT GCAGCTCCGA TAGCTACGCC AACATACACT CACCCATATC TCCCTTCGGC 
AAAACCACAC TGGACACTGG CAAGCTCTGT ATGGCCAGCA GCTTACCCAA GACTCCAGAG 
GAGATTGATG TGCCTGCCAC GATTCCAGAG AAGCCTGGGG AGCTCTCAGC CTCCAGGAAG 
CATAGCTCTG ATGTCCTGAA AGAAGAGTCC GAGGATGACC AGAACCACAG TGATGAAGAC 
AACACAGAAG TGTCACCAGT TAAATCCCCA CCAACACCAA AATCGTTCTT AAACAGAACC 
ATTACAGAGT TTCCATTCTT TAACTACAAC AATGGAATCC TGATGACCAC GTGTCGTGAT 
CTGGTGGACA ATAACCGCAG CACTCTCTCA GCTACTTCTG CCTTTGCCAT AGCGACTGCA 
GGAGCCAATG AAGGCCCCTC AAACAAGGAG GTGTTTCGAA GGATGTCTTT GGCCAACACA 
GGCTTTTCCT CTGACCAGAG AAATATCGAC AAGGAGTTTG TGATCCGCAG AGCTGCCACC 
AATCGTGTGC TGAATGTGCT CCGCCACTGG GTCACCAAGC ACACCCAGGA CTTTGATACC 
GACGATACGC TCAAATACAG GGTGATCTGT TTCCTGGAGG AGGTCATGCA TGACCCAGAC 
CTTCTGACCC AAGAGCGAAA GGCAGCAGCC AACATCATCA GGACTCTGAC CCTAGAAGAA 
ACAACTGAAC AACATAGCAT GCTGGAAGAA GTCATACTGA TGACGGAGGG TGTGAAGACT 
GAGCCCTTCG AAAACCACCC AGCCCTGGAG ATAGCAGAGC AGCTGACCCT GCTGGATCAC 
CTTGTCTTCA AGAGTATTCC TTATGAAGAG TTCTTTGGCC AGGGCTGGAT GAAGGCCGAG 
AAGTATGAAA GGACACCTTA CATTATGAAA ACCACCAAAC ACTTCAACCA TGTCAGTAAC 
TTCATTGCTT CAGAAATTAT CCGAAACGAG GACATCAGCG CAAGGGCAAG CGCCATCGAG 
AAGTGGGTGG CTGTGGCCGA CATATGTCGC TGCCTGCACA ACTACAATGC TGTGCTGGAG 
ATCACCTCCT CCATCAACCG CAGCGCCATC TTCCGTCTCA AAAAGACATG GCTCAAAGTT 
TCTAAGCAGA CGAAATCTTT GCTTGACAAG CTCCAAAAGC TTGTGTCATC AGATGGCAGA 
TTTAAGAACC TCAGAGAGTC TTTGAGAAAT TGTGATCCAC CCTGTGTCCC TTACCTGGGG 
ATGTATCTCA CCGACTTGGT GTTCATCGAG GAGGGGACAC CCAATTACAC AGAGGACGGC 
CTGGTCAACT TCTCCAAGAT GAGAATGATC TCCCATATTA TCCGAGAGAT TCGCCAGTTT 
CAGCAGACTA CCTACAAAAT CGACCCCCAG CCAAAGGTAA TTCAGTACTT ACTGGATGAA 
TCTTTCATGT TGGACGAAGA AAGCCTGTAT GAGTCTTCGC TCCTAATCGA ACCAAAACTC 
CCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035375.1
CDS	224..4012
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRIQSSNKAGSADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	NM_011245.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011245.2

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ATTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGTTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCTG CGATCGCTCG CGCCAGCTAC AAGATACTGG CTACAGAACA TGAGGCGCTT 
ATGCAGAAGT ACTTACACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAGCAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGGTAACT 
ATTACAAACC TGATCAAGGA CAATGATCGT ATCCAATCCA GCAACAAAGC CGGTTCTGCT 
GATGATGAGG ACAGTGACAT CAAGAAAATT AAGAAGGTTC AGAGCTTCCT TCGGGGATGG 
CTGTGCCGGC GAAAGTGGAA GAACATCATC CAGGACTACA TCCGGTCTCC CCACGCGGAC 
AGCATGCGCA AGAGGAACCA GGTGGTGTTC AGCATGCTGG AGGCCGAGGC TGAGTACGTC 
CAGCAGCTAC ACATCCTTGT CAACAACTTC CTGCGCCCAC TGCGCATGGC CGCCAGCTCT 
AAGAAACCCC CTATAACACA CGACGACGTC AGCAGTATCT TTCTGAACAG CGAGACCATC 
ATGTTCCTGC ATCAGATCTT CTACCAAGGC CTGAAGGCCC GTATCTCCAG CTGGCCCACC 
CTGGTGCTGG CGGACCTGTT CGATATCCTG CTGCCCATGC TGAACATCTA CCAGGAGTTC 
GTCCGCAACC ACCAGTACAG CCTCCAGATC CTTGCTCACT GCAAGCAAAA CCGGGACTTT 
GACAAGCTCC TCAAGCAGTA CGAGGCCAAG CCAGACTGCG AGGAGCGCAC ACTGGAGACC 
TTCCTCACCT ACCCAATGTT CCAGATCCCC AGGTACATCC TGACACTCCA TGAGCTGCTG 
GCCCACACAC CTCATGAGCA TGTGGAGCGC AACAGCCTGG ACTATGCCAA ATCCAAACTA 
GAGGAACTGT CCAGGATCAT GCATGACGAA GTCAGTGAAA CCGAGAACAT CCGCAAAAAC 
CTGGCCATTG AGCGTATGAT CACGGAGGGC TGTGAGATCC TCCTGGACAC CAGCCAGACC 
TTCGTGCGCC AAGGTTCCCT CATGCAGATG TCCTTGTCTG AAAAGAGCAA GTCCTCTAGG 
GGCCGCCTGG GGTCTCTGTC CACTAAGAAA GAGGGTGAGC GCCAGTGTTT CCTATTCTCC 
AAGCATCTCA TTATCTGCAC CAGAGGCTCT GGTGGGAAAC TGCACCTAAC CAAGAATGGA 
GTCATATCCC TCATTGACTG CACCTTACTG GATGAACCCG AAAACCTGGA CGACGAAGCC 
AAAGGAGCCG GACCAGAGAT AGAACACCTG GAGTTTAAGA TCGGGGTGGA GCCAAAGGAC 
TCCCTACCCT TCACTGTCAT CCTGGTAGCC TCAACCAGGC AGGAGAAGGC AGCATGGACC 
AGTGACATCA TCCAGTGCGT GGATAATATC CGCTGCAATG GGCTCATGAT GAATGCCTTT 
GAAGAAAATT CCAAGGTCAC CGTGCCTCAG ATGATCAAGT CTGACGCTTC CTTATATTGT 
GATGATGTTG ACATTCGCTT CAGCAAAACC ATGAATTCTT GCAAAGTGCT TCAGATCCGC 
TATGCCAGCG TGGAGCGCCT GCTGGAGCGC CTGACTGATC TTCGCTTCCT GAGCATTGAC 
TTTCTCAACA CCTTCCTGCA TTCCTATCGC GTCTTCACCA ATGCTATGGT GGTCCTGGAC 
AAGCTGATCA ATATCTACAG AAAACCCATG AGTGCGATTC CTGCCAGGTC CCTGGAACTC 
TTGTTTTCCA GCAGCCACAA CGCCAAGCTT CTGTACGGAG ATGCCCCTAA GTCCCCTCGC 
GCCAGCCGCA AGTTCTCCTC GCCACCGCCC TTGGCCATCG GGACGTCATC CCCCAGCCGC 
CGTCGGAAGC TGTCCCTCAA CATCCCCATC ATCACAGGCG GCAAGGCGCT GGAACTGGCT 
TCCCTTGGGT GCAGCTCCGA TAGCTACGCC AACATACACT CACCCATATC TCCCTTCGGC 
AAAACCACAC TGGACACTGG CAAGCTCTGT ATGGCCAGCA GCTTACCCAA GACTCCAGAG 
GAGATTGATG TGCCTGCCAC GATTCCAGAG AAGCCTGGGG AGCTCTCAGC CTCCAGGAAG 
CATAGCTCTG ATGTCCTGAA AGAAGAGTCC GAGGATGACC AGAACCACAG TGATGAAGAC 
AACACAGAAG TGTCACCAGT TAAATCCCCA CCAACACCAA AATCGTTCTT AAACAGAACC 
ATTACAGAGT TTCCATTCTT TAACTACAAC AATGGAATCC TGATGACCAC GTGTCGTGAT 
CTGGTGGACA ATAACCGCAG CACTCTCTCA GCTACTTCTG CCTTTGCCAT AGCGACTGCA 
GGAGCCAATG AAGGCCCCTC AAACAAGGAG GTGTTTCGAA GGATGTCTTT GGCCAACACA 
GGCTTTTCCT CTGACCAGAG AAATATCGAC AAGGAGTTTG TGATCCGCAG AGCTGCCACC 
AATCGTGTGC TGAATGTGCT CCGCCACTGG GTCACCAAGC ACACCCAGGA CTTTGATACC 
GACGATACGC TCAAATACAG GGTGATCTGT TTCCTGGAGG AGGTCATGCA TGACCCAGAC 
CTTCTGACCC AAGAGCGAAA GGCAGCAGCC AACATCATCA GGACTCTGAC CCTAGAAGAA 
ACAACTGAAC AACATAGCAT GCTGGAAGAA GTCATACTGA TGACGGAGGG TGTGAAGACT 
GAGCCCTTCG AAAACCACCC AGCCCTGGAG ATAGCAGAGC AGCTGACCCT GCTGGATCAC 
CTTGTCTTCA AGAGTATTCC TTATGAAGAG TTCTTTGGCC AGGGCTGGAT GAAGGCCGAG 
AAGTATGAAA GGACACCTTA CATTATGAAA ACCACCAAAC ACTTCAACCA TGTCAGTAAC 
TTCATTGCTT CAGAAATTAT CCGAAACGAG GACATCAGCG CAAGGGCAAG CGCCATCGAG 
AAGTGGGTGG CTGTGGCCGA CATATGTCGC TGCCTGCACA ACTACAATGC TGTGCTGGAG 
ATCACCTCCT CCATCAACCG CAGCGCCATC TTCCGTCTCA AAAAGACATG GCTCAAAGTT 
TCTAAGCAGA CGAAATCTTT GCTTGACAAG CTCCAAAAGC TTGTGTCATC AGATGGCAGA 
TTTAAGAACC TCAGAGAGTC TTTGAGAAAT TGTGATCCAC CCTGTGTCCC TTACCTGGGG 
ATGTATCTCA CCGACTTGGT GTTCATCGAG GAGGGGACAC CCAATTACAC AGAGGACGGC 
CTGGTCAACT TCTCCAAGAT GAGAATGATC TCCCATATTA TCCGAGAGAT TCGCCAGTTT 
CAGCAGACTA CCTACAAAAT CGACCCCCAG CCAAAGGTAA TTCAGTACTT ACTGGATGAA 
TCTTTCATGT TGGACGAAGA AAGCCTGTAT GAGTCTTCGC TCCTAATCGA ACCAAAACTC 
CCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( NM_001357743.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344672.1 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 3, mRNA.

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CloneID	OMu42600
Clone ID Related Accession (Same CDS sequence)	NM_001357743.1 , XM_006510875.3
Accession Version	NM_001357743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC102545.9. On Nov 4, 2017 this sequence version replaced XM_006510875.3. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is 2 aa shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.24957.1, SRR1660821.190629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ATTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGTTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCTG CGATCGCTCG CGCCAGCTAC AAGATACTGG CTACAGAACA TGAGGCGCTT 
ATGCAGAAGT ACTTACACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAGCAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA TCGTATCCAA TCCAGCAACA AAGCCGGTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGGCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT GTTCAGCATG CTGGAGGCCG AGGCTGAGTA CGTCCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CCAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGATAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTACGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCTCATG AGCATGTGGA GCGCAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAAACCGAGA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACGGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GATGTCCTTG TCTGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCACTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTATT CTCCAAGCAT 
CTCATTATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCCGGACCAG AGATAGAACA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCTA 
CCCTTCACTG TCATCCTGGT AGCCTCAACC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGGCTCA TGATGAATGC CTTTGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA AACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACT GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGCGTCTTC ACCAATGCTA TGGTGGTCCT GGACAAGCTG 
ATCAATATCT ACAGAAAACC CATGAGTGCG ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CTAAGTCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCACC GCCCTTGGCC ATCGGGACGT CATCCCCCAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGCGGCAAGG CGCTGGAACT GGCTTCCCTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCTCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCTTAC CCAAGACTCC AGAGGAGATT 
GATGTGCCTG CCACGATTCC AGAGAAGCCT GGGGAGCTCT CAGCCTCCAG GAAGCATAGC 
TCTGATGTCC TGAAAGAAGA GTCCGAGGAT GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCAC CAGTTAAATC CCCACCAACA CCAAAATCGT TCTTAAACAG AACCATTACA 
GAGTTTCCAT TCTTTAACTA CAACAATGGA ATCCTGATGA CCACGTGTCG TGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTGTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGT 
GTGCTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGTTTCCTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAACC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTGCTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGGC CGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG TAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AGCGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCTGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGT CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGTCTTTGAG AAATTGTGAT CCACCCTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA GTTTCAGCAG 
ACTACCTACA AAATCGACCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAAAGCCT GTATGAGTCT TCGCTCCTAA TCGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344672.1
CDS	224..4006
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEITNLIKDNDRIQSSNKAGSADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	NM_001357743.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357743.1

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ATTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGTTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCTG CGATCGCTCG CGCCAGCTAC AAGATACTGG CTACAGAACA TGAGGCGCTT 
ATGCAGAAGT ACTTACACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAGCAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA TCGTATCCAA TCCAGCAACA AAGCCGGTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGGCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT GTTCAGCATG CTGGAGGCCG AGGCTGAGTA CGTCCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CCAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGATAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTACGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCTCATG AGCATGTGGA GCGCAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAAACCGAGA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACGGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GATGTCCTTG TCTGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCACTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTATT CTCCAAGCAT 
CTCATTATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCCGGACCAG AGATAGAACA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCTA 
CCCTTCACTG TCATCCTGGT AGCCTCAACC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGGCTCA TGATGAATGC CTTTGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA AACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACT GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGCGTCTTC ACCAATGCTA TGGTGGTCCT GGACAAGCTG 
ATCAATATCT ACAGAAAACC CATGAGTGCG ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CTAAGTCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCACC GCCCTTGGCC ATCGGGACGT CATCCCCCAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGCGGCAAGG CGCTGGAACT GGCTTCCCTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCTCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCTTAC CCAAGACTCC AGAGGAGATT 
GATGTGCCTG CCACGATTCC AGAGAAGCCT GGGGAGCTCT CAGCCTCCAG GAAGCATAGC 
TCTGATGTCC TGAAAGAAGA GTCCGAGGAT GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCAC CAGTTAAATC CCCACCAACA CCAAAATCGT TCTTAAACAG AACCATTACA 
GAGTTTCCAT TCTTTAACTA CAACAATGGA ATCCTGATGA CCACGTGTCG TGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTGTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGT 
GTGCTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGTTTCCTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAACC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTGCTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGGC CGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG TAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AGCGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCTGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGT CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGTCTTTGAG AAATTGTGAT CCACCCTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA GTTTCAGCAG 
ACTACCTACA AAATCGACCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAAAGCCT GTATGAGTCT TCGCTCCTAA TCGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasgrf1 ( XM_006510875.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510938.1 Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42600
Clone ID Related Accession (Same CDS sequence)	NM_001357743.1 , XM_006510875.3
Accession Version	XM_006510875.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510875.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ATTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGTTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCTG CGATCGCTCG CGCCAGCTAC AAGATACTGG CTACAGAACA TGAGGCGCTT 
ATGCAGAAGT ACTTACACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAGCAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA TCGTATCCAA TCCAGCAACA AAGCCGGTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGGCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT GTTCAGCATG CTGGAGGCCG AGGCTGAGTA CGTCCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CCAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGATAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTACGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCTCATG AGCATGTGGA GCGCAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAAACCGAGA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACGGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GATGTCCTTG TCTGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCACTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTATT CTCCAAGCAT 
CTCATTATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCCGGACCAG AGATAGAACA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCTA 
CCCTTCACTG TCATCCTGGT AGCCTCAACC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGGCTCA TGATGAATGC CTTTGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA AACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACT GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGCGTCTTC ACCAATGCTA TGGTGGTCCT GGACAAGCTG 
ATCAATATCT ACAGAAAACC CATGAGTGCG ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CTAAGTCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCACC GCCCTTGGCC ATCGGGACGT CATCCCCCAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGCGGCAAGG CGCTGGAACT GGCTTCCCTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCTCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCTTAC CCAAGACTCC AGAGGAGATT 
GATGTGCCTG CCACGATTCC AGAGAAGCCT GGGGAGCTCT CAGCCTCCAG GAAGCATAGC 
TCTGATGTCC TGAAAGAAGA GTCCGAGGAT GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCAC CAGTTAAATC CCCACCAACA CCAAAATCGT TCTTAAACAG AACCATTACA 
GAGTTTCCAT TCTTTAACTA CAACAATGGA ATCCTGATGA CCACGTGTCG TGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTGTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGT 
GTGCTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGTTTCCTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAACC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTGCTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGGC CGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG TAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AGCGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCTGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGT CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGTCTTTGAG AAATTGTGAT CCACCCTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA GTTTCAGCAG 
ACTACCTACA AAATCGACCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAAAGCCT GTATGAGTCT TCGCTCCTAA TCGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510938.1
CDS	513..4295
Translation	MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEGSICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEITNLIKDNDRIQSSNKAGSADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKDSLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKPMSAIPARSLELLFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYANIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKEVFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKLPT

Target ORF information:

RefSeq Version	XM_006510875.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510875.3

ORF Insert Sequence:

ATGCAGAAAG CCATCCGGCT TAACGATGGC CACGTCGTGA CCCTGGGACT GCTGGCACAG 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGAGGAGTG CGGACAACCC AAAATGGCAA 
ACCAAGTGGT TTGCCCTGCT CCAGAACCTG CTTTTCTACT TCGAAAGCGA CTCCAGCCCT 
CGGCCCTCGG GGCTCTACCT GCTCGAGGGA AGTATCTGTA AACGCGCGCC CTCCCCCAAG 
CGAGGGACCT CCTCCAAGGA GTCCGGCGAG AAACAGCAAC ATTACTTCAC AGTGAACTTC 
TCCAATGACA GCCAGAAGAC CTTGGAGTTG AGGACCGAGG ATGCCAAGGA CTGTGACGAG 
TGGGTGGCTG CGATCGCTCG CGCCAGCTAC AAGATACTGG CTACAGAACA TGAGGCGCTT 
ATGCAGAAGT ACTTACACCT GCTGCAGGTA GTGGAGACAG AGAAGACTGT GGCTAAGCAG 
CTGCGCCAGC AGCTTGAGGA CGGTGAGGTT GAGATCGAGC GCCTGAAGAC GGAGATTACA 
AACCTGATCA AGGACAATGA TCGTATCCAA TCCAGCAACA AAGCCGGTTC TGCTGATGAT 
GAGGACAGTG ACATCAAGAA AATTAAGAAG GTTCAGAGCT TCCTTCGGGG ATGGCTGTGC 
CGGCGAAAGT GGAAGAACAT CATCCAGGAC TACATCCGGT CTCCCCACGC GGACAGCATG 
CGCAAGAGGA ACCAGGTGGT GTTCAGCATG CTGGAGGCCG AGGCTGAGTA CGTCCAGCAG 
CTACACATCC TTGTCAACAA CTTCCTGCGC CCACTGCGCA TGGCCGCCAG CTCTAAGAAA 
CCCCCTATAA CACACGACGA CGTCAGCAGT ATCTTTCTGA ACAGCGAGAC CATCATGTTC 
CTGCATCAGA TCTTCTACCA AGGCCTGAAG GCCCGTATCT CCAGCTGGCC CACCCTGGTG 
CTGGCGGACC TGTTCGATAT CCTGCTGCCC ATGCTGAACA TCTACCAGGA GTTCGTCCGC 
AACCACCAGT ACAGCCTCCA GATCCTTGCT CACTGCAAGC AAAACCGGGA CTTTGACAAG 
CTCCTCAAGC AGTACGAGGC CAAGCCAGAC TGCGAGGAGC GCACACTGGA GACCTTCCTC 
ACCTACCCAA TGTTCCAGAT CCCCAGGTAC ATCCTGACAC TCCATGAGCT GCTGGCCCAC 
ACACCTCATG AGCATGTGGA GCGCAACAGC CTGGACTATG CCAAATCCAA ACTAGAGGAA 
CTGTCCAGGA TCATGCATGA CGAAGTCAGT GAAACCGAGA ACATCCGCAA AAACCTGGCC 
ATTGAGCGTA TGATCACGGA GGGCTGTGAG ATCCTCCTGG ACACCAGCCA GACCTTCGTG 
CGCCAAGGTT CCCTCATGCA GATGTCCTTG TCTGAAAAGA GCAAGTCCTC TAGGGGCCGC 
CTGGGGTCTC TGTCCACTAA GAAAGAGGGT GAGCGCCAGT GTTTCCTATT CTCCAAGCAT 
CTCATTATCT GCACCAGAGG CTCTGGTGGG AAACTGCACC TAACCAAGAA TGGAGTCATA 
TCCCTCATTG ACTGCACCTT ACTGGATGAA CCCGAAAACC TGGACGACGA AGCCAAAGGA 
GCCGGACCAG AGATAGAACA CCTGGAGTTT AAGATCGGGG TGGAGCCAAA GGACTCCCTA 
CCCTTCACTG TCATCCTGGT AGCCTCAACC AGGCAGGAGA AGGCAGCATG GACCAGTGAC 
ATCATCCAGT GCGTGGATAA TATCCGCTGC AATGGGCTCA TGATGAATGC CTTTGAAGAA 
AATTCCAAGG TCACCGTGCC TCAGATGATC AAGTCTGACG CTTCCTTATA TTGTGATGAT 
GTTGACATTC GCTTCAGCAA AACCATGAAT TCTTGCAAAG TGCTTCAGAT CCGCTATGCC 
AGCGTGGAGC GCCTGCTGGA GCGCCTGACT GATCTTCGCT TCCTGAGCAT TGACTTTCTC 
AACACCTTCC TGCATTCCTA TCGCGTCTTC ACCAATGCTA TGGTGGTCCT GGACAAGCTG 
ATCAATATCT ACAGAAAACC CATGAGTGCG ATTCCTGCCA GGTCCCTGGA ACTCTTGTTT 
TCCAGCAGCC ACAACGCCAA GCTTCTGTAC GGAGATGCCC CTAAGTCCCC TCGCGCCAGC 
CGCAAGTTCT CCTCGCCACC GCCCTTGGCC ATCGGGACGT CATCCCCCAG CCGCCGTCGG 
AAGCTGTCCC TCAACATCCC CATCATCACA GGCGGCAAGG CGCTGGAACT GGCTTCCCTT 
GGGTGCAGCT CCGATAGCTA CGCCAACATA CACTCACCCA TATCTCCCTT CGGCAAAACC 
ACACTGGACA CTGGCAAGCT CTGTATGGCC AGCAGCTTAC CCAAGACTCC AGAGGAGATT 
GATGTGCCTG CCACGATTCC AGAGAAGCCT GGGGAGCTCT CAGCCTCCAG GAAGCATAGC 
TCTGATGTCC TGAAAGAAGA GTCCGAGGAT GACCAGAACC ACAGTGATGA AGACAACACA 
GAAGTGTCAC CAGTTAAATC CCCACCAACA CCAAAATCGT TCTTAAACAG AACCATTACA 
GAGTTTCCAT TCTTTAACTA CAACAATGGA ATCCTGATGA CCACGTGTCG TGATCTGGTG 
GACAATAACC GCAGCACTCT CTCAGCTACT TCTGCCTTTG CCATAGCGAC TGCAGGAGCC 
AATGAAGGCC CCTCAAACAA GGAGGTGTTT CGAAGGATGT CTTTGGCCAA CACAGGCTTT 
TCCTCTGACC AGAGAAATAT CGACAAGGAG TTTGTGATCC GCAGAGCTGC CACCAATCGT 
GTGCTGAATG TGCTCCGCCA CTGGGTCACC AAGCACACCC AGGACTTTGA TACCGACGAT 
ACGCTCAAAT ACAGGGTGAT CTGTTTCCTG GAGGAGGTCA TGCATGACCC AGACCTTCTG 
ACCCAAGAGC GAAAGGCAGC AGCCAACATC ATCAGGACTC TGACCCTAGA AGAAACAACT 
GAACAACATA GCATGCTGGA AGAAGTCATA CTGATGACGG AGGGTGTGAA GACTGAGCCC 
TTCGAAAACC ACCCAGCCCT GGAGATAGCA GAGCAGCTGA CCCTGCTGGA TCACCTTGTC 
TTCAAGAGTA TTCCTTATGA AGAGTTCTTT GGCCAGGGCT GGATGAAGGC CGAGAAGTAT 
GAAAGGACAC CTTACATTAT GAAAACCACC AAACACTTCA ACCATGTCAG TAACTTCATT 
GCTTCAGAAA TTATCCGAAA CGAGGACATC AGCGCAAGGG CAAGCGCCAT CGAGAAGTGG 
GTGGCTGTGG CCGACATATG TCGCTGCCTG CACAACTACA ATGCTGTGCT GGAGATCACC 
TCCTCCATCA ACCGCAGCGC CATCTTCCGT CTCAAAAAGA CATGGCTCAA AGTTTCTAAG 
CAGACGAAAT CTTTGCTTGA CAAGCTCCAA AAGCTTGTGT CATCAGATGG CAGATTTAAG 
AACCTCAGAG AGTCTTTGAG AAATTGTGAT CCACCCTGTG TCCCTTACCT GGGGATGTAT 
CTCACCGACT TGGTGTTCAT CGAGGAGGGG ACACCCAATT ACACAGAGGA CGGCCTGGTC 
AACTTCTCCA AGATGAGAAT GATCTCCCAT ATTATCCGAG AGATTCGCCA GTTTCAGCAG 
ACTACCTACA AAATCGACCC CCAGCCAAAG GTAATTCAGT ACTTACTGGA TGAATCTTTC 
ATGTTGGACG AAGAAAGCCT GTATGAGTCT TCGCTCCTAA TCGAACCAAA ACTCCCCACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Rasgrf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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