Rab6a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab6a
Entrez Gene ID	19346
Full Name	RAB6A, member RAS oncogene family
Synonyms	2610028L11Rik,AA419671,Rab6
General protein information	Preferred Names RAB6A, member RAS oncogene family Names ras-related protein Rab-6A RAB6, member RAS oncogene family rab-6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163663.1	NP_001157135.1	ras-related protein Rab-6A isoform 1
NM_024287.4	NP_077249.1	ras-related protein Rab-6A isoform 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	rab-6.1	NP_498993.1
Arabidopsis thaliana (thale cress)	RAB6A	NP_181989.1
Oryza sativa (rice)	Os07g0496000	NP_001059699.1
Pan troglodytes (chimpanzee)	RAB6A	XP_003313253.2
Mus musculus (house mouse)	Rab6a	NP_001157135.1
Gallus gallus (chicken)	RAB6A	NP_001038105.1
Xenopus tropicalis (tropical clawed frog)	LOC100124772	NP_001096221.1
Homo sapiens (human)	RAB6A	NP_002860.2
	RAB6A	XP_001115437.1
Rattus norvegicus (Norway rat)	Rab6a	NP_445818.1
Danio rerio (zebrafish)	zgc:63637	NP_998530.1

Gene Ontology
Bibliography

Related articles in PubMed

Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.
Miserey-Lenkei S, Bousquet H, Pylypenko O, Bardin S, Dimitrov A, Bressanelli G, Bonifay R, Fraisier V, Guillou C, Bougeret C, Houdusse A, Echard A, Goud B
Nature communications8(1)1254(2017 Nov)

Routing of the RAB6 secretory pathway towards the lysosome related organelle of melanocytes.
Patwardhan A, Bardin S, Miserey-Lenkei S, Larue L, Goud B, Raposo G, Delevoye C
Nature communications815835(2017 Jun)

Neuronal expression of ILEI/FAM3C and its reduction in Alzheimer's disease.
Liu L, Watanabe N, Akatsu H, Nishimura M
Neuroscience330236-46(2016 Aug)

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data reveal that Rab6a not only participates in modulating the organization of oocyte organelles, but also is a novel regulator of meiotic apparatus in mammalian oocytes.
Title: Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation.

Rab6a knockdown in oocytes may compromise the interaction between kinetochore and microtubule, which in turn leads to the activation of spindle assembly checkpoint signal, and, as a result, chromosome misalignment and spindle defects are established during meiosis, causing the production of aneuploid eggs and persistent defects in embryos.
Title: Rab6a is a novel regulator of meiotic apparatus and maturational progression in mouse oocytes.

Our study shows that RAB6 is an essential gene required for normal embryonic development
Title: Phenotypic characterisation of RAB6A knockout mouse embryonic fibroblasts.

several analyses of vesicular transport demonstrated that Rab6A and BICD2 play crucial roles in Golgi tubule fusion with the endoplasmic reticulum (ER) in brefeldin A (BFA)-treated cells
Title: Reconstitution of the targeting of Rab6A to the Golgi apparatus in semi-intact HeLa cells: A role of BICD2 in stabilizing Rab6A on Golgi membranes and a concerted role of Rab6A/BICD2 interactions in Golgi-to-ER retrograde transport.

Rab6 GTPase was required during the phagosome maturation by its interaction with bicaudal-D1 (BICD1) protein. Further data presented that the Rab6 GTPase-regulated phagocytosis could influence the proliferation of Staphylococcus aureus in macrophages.
Title: The role of Rab6 GTPase in the maturation of phagosome against Staphylococcus aureus.

The following Rab6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08166	NM_024287.4 Latest version!	Mus musculus RAB6A, member RAS oncogene family (Rab6a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu09294	NM_001163663.1 Latest version!	Mus musculus RAB6A, member RAS oncogene family (Rab6a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$146.30 $209.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab6a ( NM_024287.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077249.1 Mus musculus RAB6A, member RAS oncogene family (Rab6a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08166
Clone ID Related Accession (Same CDS sequence)	NM_024287.4
Accession Version	NM_024287.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-6A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124531.5, AK084131.1, CX734203.1 and BP765476.1. On Jul 28, 2009 this sequence version replaced NM_024287.3. Transcript Variant: Variants 1 and 2 include either one of two duplicated, alternatively spliced coding exons and are the same length. The resulting proteins (isoforms 1 and 2, respectively) are the same length but differ at three amino acid positions. This variant (2) encodes isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169842.1, AK083262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCGG GCGGAGACTT CGGGAATCCG CTGAGGAAAT TCAAGCTGGT GTTCCTGGGA 
GAGCAGAGCG TTGGAAAGAC GTCCTTGATC ACCCGATTCA TGTATGACAG TTTTGACAAC 
ACCTATCAGG CAACAATTGG CATTGACTTC TTATCAAAAA CAATGTACTT GGAGGATAGA 
ACCGTGCGAT TGCAATTATG GGACACAGCA GGTCAAGAGC GGTTCAGGAG CTTGATTCCT 
AGCTACATTC GAGACTCCAC TGTGGCAGTT GTTGTTTATG ATATCACAAA TGTTAACTCA 
TTCCAGCAAA CTACAAAATG GATTGATGAT GTCAGAACAG AGAGAGGAAG TGATGTCATC 
ATCATGCTAG TAGGAAATAA AACAGATCTT GCTGATAAGA GGCAAGTGTC AATTGAGGAG 
GGAGAGAGGA AAGCCAAAGA GCTGAATGTT ATGTTTATTG AAACCAGTGC AAAAGCAGGA 
TACAATGTAA AGCAGCTTTT CCGGCGTGTT GCTGCAGCTT TACCTGGAAT GGAAAGCACA 
CAGGACAGAA GCAGAGAAGA CATGATTGAC ATAAAACTGG AAAAGCCTCA GGAGCAACCA 
GTCAATGAAG GCGGCTGTTC CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077249.1
CDS	639..1265
Translation	MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVNEGGCSC

Target ORF information:

RefSeq Version	NM_024287.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB6A, member RAS oncogene family (Rab6a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024287.4

ORF Insert Sequence:

ATGTCCGCGG GCGGAGACTT CGGGAATCCG CTGAGGAAAT TCAAGCTGGT GTTCCTGGGA 
GAGCAGAGCG TTGGAAAGAC GTCCTTGATC ACCCGATTCA TGTATGACAG TTTTGACAAC 
ACCTATCAGG CAACAATTGG CATTGACTTC TTATCAAAAA CAATGTACTT GGAGGATAGA 
ACCGTGCGAT TGCAATTATG GGACACAGCA GGTCAAGAGC GGTTCAGGAG CTTGATTCCT 
AGCTACATTC GAGACTCCAC TGTGGCAGTT GTTGTTTATG ATATCACAAA TGTTAACTCA 
TTCCAGCAAA CTACAAAATG GATTGATGAT GTCAGAACAG AGAGAGGAAG TGATGTCATC 
ATCATGCTAG TAGGAAATAA AACAGATCTT GCTGATAAGA GGCAAGTGTC AATTGAGGAG 
GGAGAGAGGA AAGCCAAAGA GCTGAATGTT ATGTTTATTG AAACCAGTGC AAAAGCAGGA 
TACAATGTAA AGCAGCTTTT CCGGCGTGTT GCTGCAGCTT TACCTGGAAT GGAAAGCACA 
CAGGACAGAA GCAGAGAAGA CATGATTGAC ATAAAACTGG AAAAGCCTCA GGAGCAACCA 
GTCAATGAAG GCGGCTGTTC CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab6a ( NM_001163663.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157135.1 Mus musculus RAB6A, member RAS oncogene family (Rab6a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09294
Clone ID Related Accession (Same CDS sequence)	NM_001163663.1
Accession Version	NM_001163663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-6A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124531.5, BE134038.1, AK084131.1, CX734203.1 and BP765476.1. Transcript Variant: Variants 1 and 2 include either one of two duplicated, alternatively spliced coding exons and are the same length. The resulting proteins (isoforms 1 and 2, respectively) are the same length but differ at three amino acid positions. This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.197399.1, SRR1660813.140565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGCGG GCGGAGACTT CGGGAATCCG CTGAGGAAAT TCAAGCTGGT GTTCCTGGGA 
GAGCAGAGCG TTGGAAAGAC GTCCTTGATC ACCCGATTCA TGTATGACAG TTTTGACAAC 
ACCTATCAGG CAACAATTGG CATTGACTTC TTATCAAAAA CAATGTACTT GGAGGATAGA 
ACCATCAGGC TGCAGCTGTG GGATACTGCG GGTCAGGAAC GTTTCCGTAG CCTCATTCCC 
AGTTACATCC GTGACTCTGC TGCAGCCGTA GTAGTTTACG ATATCACAAA TGTTAACTCA 
TTCCAGCAAA CTACAAAATG GATTGATGAT GTCAGAACAG AGAGAGGAAG TGATGTCATC 
ATCATGCTAG TAGGAAATAA AACAGATCTT GCTGATAAGA GGCAAGTGTC AATTGAGGAG 
GGAGAGAGGA AAGCCAAAGA GCTGAATGTT ATGTTTATTG AAACCAGTGC AAAAGCAGGA 
TACAATGTAA AGCAGCTTTT CCGGCGTGTT GCTGCAGCTT TACCTGGAAT GGAAAGCACA 
CAGGACAGAA GCAGAGAAGA CATGATTGAC ATAAAACTGG AAAAGCCTCA GGAGCAACCA 
GTCAATGAAG GCGGCTGTTC CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157135.1
CDS	639..1265
Translation	MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVNEGGCSC

Target ORF information:

RefSeq Version	NM_001163663.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB6A, member RAS oncogene family (Rab6a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163663.1

ORF Insert Sequence:

ATGTCCGCGG GCGGAGACTT CGGGAATCCG CTGAGGAAAT TCAAGCTGGT GTTCCTGGGA 
GAGCAGAGCG TTGGAAAGAC GTCCTTGATC ACCCGATTCA TGTATGACAG TTTTGACAAC 
ACCTATCAGG CAACAATTGG CATTGACTTC TTATCAAAAA CAATGTACTT GGAGGATAGA 
ACCATCAGGC TGCAGCTGTG GGATACTGCG GGTCAGGAAC GTTTCCGTAG CCTCATTCCC 
AGTTACATCC GTGACTCTGC TGCAGCCGTA GTAGTTTACG ATATCACAAA TGTTAACTCA 
TTCCAGCAAA CTACAAAATG GATTGATGAT GTCAGAACAG AGAGAGGAAG TGATGTCATC 
ATCATGCTAG TAGGAAATAA AACAGATCTT GCTGATAAGA GGCAAGTGTC AATTGAGGAG 
GGAGAGAGGA AAGCCAAAGA GCTGAATGTT ATGTTTATTG AAACCAGTGC AAAAGCAGGA 
TACAATGTAA AGCAGCTTTT CCGGCGTGTT GCTGCAGCTT TACCTGGAAT GGAAAGCACA 
CAGGACAGAA GCAGAGAAGA CATGATTGAC ATAAAACTGG AAAAGCCTCA GGAGCAACCA 
GTCAATGAAG GCGGCTGTTC CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.
Nature communications8(1)1254(2017 Nov)
Miserey-Lenkei S,Bousquet H,Pylypenko O,Bardin S,Dimitrov A,Bressanelli G,Bonifay R,Fraisier V,Guillou C,Bougeret C,Houdusse A,Echard A,Goud B

Routing of the RAB6 secretory pathway towards the lysosome related organelle of melanocytes.
Nature communications815835(2017 Jun)
Patwardhan A,Bardin S,Miserey-Lenkei S,Larue L,Goud B,Raposo G,Delevoye C

Neuronal expression of ILEI/FAM3C and its reduction in Alzheimer's disease.
Neuroscience330236-46(2016 Aug)
Liu L,Watanabe N,Akatsu H,Nishimura M

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W