Rab20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab20
Entrez Gene ID	19332
Full Name	RAB20, member RAS oncogene family
Synonyms	AA536966,D8Ertd350e
General protein information	Preferred Names RAB20, member RAS oncogene family Names ras-related protein Rab-20
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 5.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242042.2	XP_011240344.1	ras-related protein Rab-20 isoform X1
XM_011242042.3	XP_011240344.1	ras-related protein Rab-20 isoform X1
NM_011227.1	NP_035357.1	ras-related protein Rab-20
NM_011227.2	NP_035357.1	ras-related protein Rab-20

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rab20	NP_035357.1
Xenopus tropicalis (tropical clawed frog)	rab20	NP_001017295.1
Bos taurus (cattle)	RAB20	NP_001180018.1
Homo sapiens (human)	RAB20	NP_060287.1
Gallus gallus (chicken)	RAB20	XP_416953.2
Pan troglodytes (chimpanzee)	RAB20	XP_001137007.1
	RAB20	XP_001082373.1
Canis lupus familiaris (dog)	RAB20	XP_542668.2
Rattus norvegicus (Norway rat)	Rab20	NP_001103005.1
Danio rerio (zebrafish)	rab20	NP_997936.1

Gene Ontology
Bibliography

Related articles in PubMed

A Rab20-Dependent Membrane Trafficking Pathway Controls M.?tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Schnettger L, Rodgers A, Repnik U, Lai RP, Pei G, Verdoes M, Wilkinson RJ, Young DB, Gutierrez MG
Cell host & microbe21(5)619-628.e5(2017 May)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Inpp5e increases the Rab5 association and phosphatidylinositol 3-phosphate accumulation at the phagosome through an interaction with Rab20.
Segawa T, Hazeki K, Nigorikawa K, Morioka S, Guo Y, Takasuga S, Asanuma K, Hazeki O
The Biochemical journal464(3)365-75(2014 Dec)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Expression profiling of Rab GTPases reveals the involvement of Rab20 and Rab32 in acute brain inflammation in mice.
Liang Y, Lin S, Zou L, Zhou H, Zhang J, Su B, Wan Y
Neuroscience letters527(2)110-4(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inpp5e, through functional interactions with Rab20 on the phagosome, activates Rab5, which, in turn, increases PtdIns3P and delays phagosome acidification.
Title: Inpp5e increases the Rab5 association and phosphatidylinositol 3-phosphate accumulation at the phagosome through an interaction with Rab20.

Rabs may participate in inflammatory response in microglia during brain inflammation.
Title: Expression profiling of Rab GTPases reveals the involvement of Rab20 and Rab32 in acute brain inflammation in mice.

Rab20 is an important component of phagosome and regulates the phagosome maturation during Fc gamma receptor-mediated phagocytosis.
Title: Rab20 regulates phagosome maturation in RAW264 macrophages during Fc gamma receptor-mediated phagocytosis.

The following Rab20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07723	NM_011227.2 Latest version!	Mus musculus RAB20, member RAS oncogene family (Rab20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu68570	XM_011242042.3 Latest version!	Mus musculus RAB20, member RAS oncogene family (Rab20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu68570	XM_011242042.2	Mus musculus RAB20, member RAS oncogene family (Rab20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu07723	NM_011227.1	Mus musculus RAB20, member RAS oncogene family (Rab20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab20 ( NM_011227.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035357.1 Mus musculus RAB20, member RAS oncogene family (Rab20), mRNA.

Price & Availability↑

CloneID	OMu07723
Clone ID Related Accession (Same CDS sequence)	NM_011227.1 , NM_011227.2
Accession Version	NM_011227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X80332.1 and BG066967.2. On Mar 23, 2004 this sequence version replaced XM_134046.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB232614.1, BC089570.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGGAAGC CGGATGGGAA GATCGTACTG CTGGGGGACA TGAACGTGGG TAAAACGTCG 
CTGCTGCAGC GCTACATGGA GCGTCGCTTC CCGGACACGG TCAGCACCGT GGGCGGCGCC 
TTCTACCTGA AGCAGTGGCG TTCCTTCAAC ATCTCCATAT GGGACACCGC AGGGCGGGAG 
CAGTTTCATG GTCTGGGCTC CCTGTACTGC CGCGGAGCGG CCGCTATCAT CCTTACATAC 
GATGTGAACC ACCCACAGAG CCTGTTTGAG CTGGAGGACA GATTCCTGGG CCTGACAGAA 
ACAGCCAACA ATGACTGCCT GTTTGCCATT GTGGGGAACA AAGTGGACCT GACCTCGGAG 
CGGGACACCG AGGGCGGGGA GAAGGAAGGA CCGGCCTCTG GCAAGGTGGG CAGCTGTGTC 
TCCACCAAGG TACCCAAGCA GGTGCAGCCA GAGGATGCAG TGGCCCTTTA CAAGAAGATC 
CTGAAGTACA AGATGCTGGA TGAGAGGGAG ATGCCAGCTG CTGAGCAGAT GTGCTTCGAG 
ACCAGTGCCA AGACTGGATA CAATGTGGAC CTCCTCTTTG AAACCTTGTT TGACCTGGTG 
GTACCTATGA TCATGCGGCA GAGGGCTGAG GAGTCAGATC AGACTGTGGA TATAGCCAGC 
TGCAAAACAC CCAAACAGAC TAGATCCGGG TGTTGTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035357.1
CDS	1..702
Translation	MRKPDGKIVLLGDMNVGKTSLLQRYMERRFPDTVSTVGGAFYLKQWRSFNISIWDTAGREQFHGLGSLYCRGAAAIILTYDVNHPQSLFELEDRFLGLTETANNDCLFAIVGNKVDLTSERDTEGGEKEGPASGKVGSCVSTKVPKQVQPEDAVALYKKILKYKMLDEREMPAAEQMCFETSAKTGYNVDLLFETLFDLVVPMIMRQRAEESDQTVDIASCKTPKQTRSGCCA

Target ORF information:

RefSeq Version	NM_011227.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB20, member RAS oncogene family (Rab20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011227.1

ORF Insert Sequence:

ATGCGGAAGC CGGATGGGAA GATCGTACTG CTGGGGGACA TGAACGTGGG TAAAACGTCG 
CTGCTGCAGC GCTACATGGA GCGTCGCTTC CCGGACACGG TCAGCACCGT GGGCGGCGCC 
TTCTACCTGA AGCAGTGGCG TTCCTTCAAC ATCTCCATAT GGGACACCGC AGGGCGGGAG 
CAGTTTCATG GTCTGGGCTC CCTGTACTGC CGCGGAGCGG CCGCTATCAT CCTTACATAC 
GATGTGAACC ACCCACAGAG CCTGTTTGAG CTGGAGGACA GATTCCTGGG CCTGACAGAA 
ACAGCCAACA ATGACTGCCT GTTTGCCATT GTGGGGAACA AAGTGGACCT GACCTCGGAG 
CGGGACACCG AGGGCGGGGA GAAGGAAGGA CCGGCCTCTG GCAAGGTGGG CAGCTGTGTC 
TCCACCAAGG TACCCAAGCA GGTGCAGCCA GAGGATGCAG TGGCCCTTTA CAAGAAGATC 
CTGAAGTACA AGATGCTGGA TGAGAGGGAG ATGCCAGCTG CTGAGCAGAT GTGCTTCGAG 
ACCAGTGCCA AGACTGGATA CAATGTGGAC CTCCTCTTTG AAACCTTGTT TGACCTGGTG 
GTACCTATGA TCATGCGGCA GAGGGCTGAG GAGTCAGATC AGACTGTGGA TATAGCCAGC 
TGCAAAACAC CCAAACAGAC TAGATCCGGG TGTTGTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab20 ( NM_011227.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035357.1 Mus musculus RAB20, member RAS oncogene family (Rab20), mRNA.

Price & Availability↑

CloneID	OMu07723
Clone ID Related Accession (Same CDS sequence)	NM_011227.1 , NM_011227.2
Accession Version	NM_011227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124475.3. On Jun 18, 2019 this sequence version replaced NM_011227.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1795193.1, SRR5189685.70139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGGAAGC CGGATGGGAA GATCGTACTG CTGGGGGACA TGAACGTGGG TAAAACGTCG 
CTGCTGCAGC GCTACATGGA GCGTCGCTTC CCGGACACGG TCAGCACCGT GGGCGGCGCC 
TTCTACCTGA AGCAGTGGCG TTCCTTCAAC ATCTCCATAT GGGACACCGC AGGGCGGGAG 
CAGTTTCATG GTCTGGGCTC CCTGTACTGC CGCGGAGCGG CCGCTATCAT CCTTACATAC 
GATGTGAACC ACCCACAGAG CCTGTTTGAG CTGGAGGACA GATTCCTGGG CCTGACAGAA 
ACAGCCAACA ATGACTGCCT GTTTGCCATT GTGGGGAACA AAGTGGACCT GACCTCGGAG 
CGGGACACCG AGGGCGGGGA GAAGGAAGGA CCGGCCTCTG GCAAGGTGGG CAGCTGTGTC 
TCCACCAAGG TACCCAAGCA GGTGCAGCCA GAGGATGCAG TGGCCCTTTA CAAGAAGATC 
CTGAAGTACA AGATGCTGGA TGAGAGGGAG ATGCCAGCTG CTGAGCAGAT GTGCTTCGAG 
ACCAGTGCCA AGACTGGATA CAATGTGGAC CTCCTCTTTG AAACCTTGTT TGACCTGGTG 
GTACCTATGA TCATGCGGCA GAGGGCTGAG GAGTCAGATC AGACTGTGGA TATAGCCAGC 
TGCAAAACAC CCAAACAGAC TAGATCCGGG TGTTGTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035357.1
CDS	142..843
Translation	MRKPDGKIVLLGDMNVGKTSLLQRYMERRFPDTVSTVGGAFYLKQWRSFNISIWDTAGREQFHGLGSLYCRGAAAIILTYDVNHPQSLFELEDRFLGLTETANNDCLFAIVGNKVDLTSERDTEGGEKEGPASGKVGSCVSTKVPKQVQPEDAVALYKKILKYKMLDEREMPAAEQMCFETSAKTGYNVDLLFETLFDLVVPMIMRQRAEESDQTVDIASCKTPKQTRSGCCA

Target ORF information:

RefSeq Version	NM_011227.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB20, member RAS oncogene family (Rab20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011227.2

ORF Insert Sequence:

ATGCGGAAGC CGGATGGGAA GATCGTACTG CTGGGGGACA TGAACGTGGG TAAAACGTCG 
CTGCTGCAGC GCTACATGGA GCGTCGCTTC CCGGACACGG TCAGCACCGT GGGCGGCGCC 
TTCTACCTGA AGCAGTGGCG TTCCTTCAAC ATCTCCATAT GGGACACCGC AGGGCGGGAG 
CAGTTTCATG GTCTGGGCTC CCTGTACTGC CGCGGAGCGG CCGCTATCAT CCTTACATAC 
GATGTGAACC ACCCACAGAG CCTGTTTGAG CTGGAGGACA GATTCCTGGG CCTGACAGAA 
ACAGCCAACA ATGACTGCCT GTTTGCCATT GTGGGGAACA AAGTGGACCT GACCTCGGAG 
CGGGACACCG AGGGCGGGGA GAAGGAAGGA CCGGCCTCTG GCAAGGTGGG CAGCTGTGTC 
TCCACCAAGG TACCCAAGCA GGTGCAGCCA GAGGATGCAG TGGCCCTTTA CAAGAAGATC 
CTGAAGTACA AGATGCTGGA TGAGAGGGAG ATGCCAGCTG CTGAGCAGAT GTGCTTCGAG 
ACCAGTGCCA AGACTGGATA CAATGTGGAC CTCCTCTTTG AAACCTTGTT TGACCTGGTG 
GTACCTATGA TCATGCGGCA GAGGGCTGAG GAGTCAGATC AGACTGTGGA TATAGCCAGC 
TGCAAAACAC CCAAACAGAC TAGATCCGGG TGTTGTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab20 ( XM_011242042.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240344.1 Mus musculus RAB20, member RAS oncogene family (Rab20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68570
Clone ID Related Accession (Same CDS sequence)	XM_011242042.2 , XM_011242042.3
Accession Version	XM_011242042.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA TCCTTCTCCA AGGGCACACA GGGCGGGAGC AGTTTCATGG TCTGGGCTCC 
CTGTACTGCC GCGGAGCGGC CGCTATCATC CTTACATACG ATGTGAACCA CCCACAGAGC 
CTGTTTGAGC TGGAGGACAG ATTCCTGGGC CTGACAGAAA CAGCCAACAA TGACTGCCTG 
TTTGCCATTG TGGGGAACAA AGTGGACCTG ACCTCGGAGC GGGACACCGA GGGCGGGGAG 
AAGGAAGGAC CGGCCTCTGG CAAGGTGGGC AGCTGTGTCT CCACCAAGGT ACCCAAGCAG 
GTGCAGCCAG AGGATGCAGT GGCCCTTTAC AAGAAGATCC TGAAGTACAA GATGCTGGAT 
GAGAGGGAGA TGCCAGCTGC TGAGCAGATG TGCTTCGAGA CCAGTGCCAA GACTGGATAC 
AATGTGGACC TCCTCTTTGA AACCTTGTTT GACCTGGTGG TACCTATGAT CATGCGGCAG 
AGGGCTGAGG AGTCAGATCA GACTGTGGAT ATAGCCAGCT GCAAAACACC CAAACAGACT 
AGATCCGGGT GTTGTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240344.1
CDS	204..764
Translation	MSAILLQGHTGREQFHGLGSLYCRGAAAIILTYDVNHPQSLFELEDRFLGLTETANNDCLFAIVGNKVDLTSERDTEGGEKEGPASGKVGSCVSTKVPKQVQPEDAVALYKKILKYKMLDEREMPAAEQMCFETSAKTGYNVDLLFETLFDLVVPMIMRQRAEESDQTVDIASCKTPKQTRSGCCA

Target ORF information:

RefSeq Version	XM_011242042.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB20, member RAS oncogene family (Rab20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242042.2

ORF Insert Sequence:

ATGTCTGCGA TCCTTCTCCA AGGGCACACA GGGCGGGAGC AGTTTCATGG TCTGGGCTCC 
CTGTACTGCC GCGGAGCGGC CGCTATCATC CTTACATACG ATGTGAACCA CCCACAGAGC 
CTGTTTGAGC TGGAGGACAG ATTCCTGGGC CTGACAGAAA CAGCCAACAA TGACTGCCTG 
TTTGCCATTG TGGGGAACAA AGTGGACCTG ACCTCGGAGC GGGACACCGA GGGCGGGGAG 
AAGGAAGGAC CGGCCTCTGG CAAGGTGGGC AGCTGTGTCT CCACCAAGGT ACCCAAGCAG 
GTGCAGCCAG AGGATGCAGT GGCCCTTTAC AAGAAGATCC TGAAGTACAA GATGCTGGAT 
GAGAGGGAGA TGCCAGCTGC TGAGCAGATG TGCTTCGAGA CCAGTGCCAA GACTGGATAC 
AATGTGGACC TCCTCTTTGA AACCTTGTTT GACCTGGTGG TACCTATGAT CATGCGGCAG 
AGGGCTGAGG AGTCAGATCA GACTGTGGAT ATAGCCAGCT GCAAAACACC CAAACAGACT 
AGATCCGGGT GTTGTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab20 ( XM_011242042.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240344.1 Mus musculus RAB20, member RAS oncogene family (Rab20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68570
Clone ID Related Accession (Same CDS sequence)	XM_011242042.2 , XM_011242042.3
Accession Version	XM_011242042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA TCCTTCTCCA AGGGCACACA GGGCGGGAGC AGTTTCATGG TCTGGGCTCC 
CTGTACTGCC GCGGAGCGGC CGCTATCATC CTTACATACG ATGTGAACCA CCCACAGAGC 
CTGTTTGAGC TGGAGGACAG ATTCCTGGGC CTGACAGAAA CAGCCAACAA TGACTGCCTG 
TTTGCCATTG TGGGGAACAA AGTGGACCTG ACCTCGGAGC GGGACACCGA GGGCGGGGAG 
AAGGAAGGAC CGGCCTCTGG CAAGGTGGGC AGCTGTGTCT CCACCAAGGT ACCCAAGCAG 
GTGCAGCCAG AGGATGCAGT GGCCCTTTAC AAGAAGATCC TGAAGTACAA GATGCTGGAT 
GAGAGGGAGA TGCCAGCTGC TGAGCAGATG TGCTTCGAGA CCAGTGCCAA GACTGGATAC 
AATGTGGACC TCCTCTTTGA AACCTTGTTT GACCTGGTGG TACCTATGAT CATGCGGCAG 
AGGGCTGAGG AGTCAGATCA GACTGTGGAT ATAGCCAGCT GCAAAACACC CAAACAGACT 
AGATCCGGGT GTTGTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240344.1
CDS	203..763
Translation	MSAILLQGHTGREQFHGLGSLYCRGAAAIILTYDVNHPQSLFELEDRFLGLTETANNDCLFAIVGNKVDLTSERDTEGGEKEGPASGKVGSCVSTKVPKQVQPEDAVALYKKILKYKMLDEREMPAAEQMCFETSAKTGYNVDLLFETLFDLVVPMIMRQRAEESDQTVDIASCKTPKQTRSGCCA

Target ORF information:

RefSeq Version	XM_011242042.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB20, member RAS oncogene family (Rab20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242042.3

ORF Insert Sequence:

ATGTCTGCGA TCCTTCTCCA AGGGCACACA GGGCGGGAGC AGTTTCATGG TCTGGGCTCC 
CTGTACTGCC GCGGAGCGGC CGCTATCATC CTTACATACG ATGTGAACCA CCCACAGAGC 
CTGTTTGAGC TGGAGGACAG ATTCCTGGGC CTGACAGAAA CAGCCAACAA TGACTGCCTG 
TTTGCCATTG TGGGGAACAA AGTGGACCTG ACCTCGGAGC GGGACACCGA GGGCGGGGAG 
AAGGAAGGAC CGGCCTCTGG CAAGGTGGGC AGCTGTGTCT CCACCAAGGT ACCCAAGCAG 
GTGCAGCCAG AGGATGCAGT GGCCCTTTAC AAGAAGATCC TGAAGTACAA GATGCTGGAT 
GAGAGGGAGA TGCCAGCTGC TGAGCAGATG TGCTTCGAGA CCAGTGCCAA GACTGGATAC 
AATGTGGACC TCCTCTTTGA AACCTTGTTT GACCTGGTGG TACCTATGAT CATGCGGCAG 
AGGGCTGAGG AGTCAGATCA GACTGTGGAT ATAGCCAGCT GCAAAACACC CAAACAGACT 
AGATCCGGGT GTTGTGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Rab20-Dependent Membrane Trafficking Pathway Controls M.?tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Cell host & microbe21(5)619-628.e5(2017 May)
Schnettger L,Rodgers A,Repnik U,Lai RP,Pei G,Verdoes M,Wilkinson RJ,Young DB,Gutierrez MG

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Inpp5e increases the Rab5 association and phosphatidylinositol 3-phosphate accumulation at the phagosome through an interaction with Rab20.
The Biochemical journal464(3)365-75(2014 Dec)
Segawa T,Hazeki K,Nigorikawa K,Morioka S,Guo Y,Takasuga S,Asanuma K,Hazeki O

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
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Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Expression profiling of Rab GTPases reveals the involvement of Rab20 and Rab32 in acute brain inflammation in mice.
Neuroscience letters527(2)110-4(2012 Oct)
Liang Y,Lin S,Zou L,Zhou H,Zhang J,Su B,Wan Y

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Rab20 cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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