Ptch2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptch2
Entrez Gene ID	19207
Full Name	patched 2
Synonyms	ptc2
General protein information	Preferred Names patched 2 Names protein patched homolog 2 patched homolog 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D1\|4 53.41 cM
Summary	This gene encodes a member of the patched family of transmembrane receptor proteins. The encoded protein may be a functional receptor for the morphogen sonic hedgehog (Shh) and is reportedly involved in limb and skin development. Homozygous mutant mice for this gene exhibit hair loss and epidermal hyperplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240470.2	XP_011238772.1	protein patched homolog 2 isoform X1
XM_011240472.1	XP_011238774.1	protein patched homolog 2 isoform X3
XM_011240476.2	XP_011238778.1	protein patched homolog 2 isoform X4
NM_008958.3	NP_032984.1	protein patched homolog 2 isoform 1
XM_011240471.2	XP_011238773.1	protein patched homolog 2 isoform X2
NM_001312903.1	NP_001299832.1	protein patched homolog 2 isoform 2
XM_011240473.1	XP_011238775.1	protein patched homolog 2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PTCH2	NP_003729.3
Canis lupus familiaris (dog)	PTCH2	XP_539636.3
Mus musculus (house mouse)	Ptch2	NP_032984.1
Pan troglodytes (chimpanzee)	PTCH2	XP_003954411.1
Bos taurus (cattle)	PTCH2	XP_005204949.1

Gene Ontology
Bibliography

Related articles in PubMed

Activated Hedgehog-GLI Signaling Causes Congenital Ureteropelvic Junction Obstruction.
Sheybani-Deloui S, Chi L, Staite MV, Cain JE, Nieman BJ, Henkelman RM, Wainwright BJ, Potter SS, Bagli DJ, Lorenzo AJ, Rosenblum ND
Journal of the American Society of Nephrology : JASN(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings support a model in which Ptch1/2 mediate secretion of a Smo-inhibitory cholesterol precursor.
Title: Patched1 and Patched2 inhibit Smoothened non-cell autonomously.

Ptch2(-/-) niche cells show hyperactive noncanonical HH signaling, resulting in reduced production of essential HSC regulators (Scf, Cxcl12, and Jag1) and depletion of osteoblasts.
Title: Ptch2 loss drives myeloproliferation and myeloproliferative neoplasm progression.

Ptch2 is a functional Shh receptor that shares overlapping functions with Ptch1 in Smo regulation and limb development.
Title: Ptch2 shares overlapping functions with Ptch1 in Smo regulation and limb development.

Ptch1(-/-);Ptch2(-/-) cells cannot further activate the Shh response, demonstrating that Ptch2 mediates the response to Shh in the absence of Ptch1.
Title: Ptch2 mediates the Shh response in Ptch1-/- cells.

Our studies indicate that concomitant loss of Ptch1 and Ptch2 activity inhibits epidermal lineage specification and differentiation.
Title: Patched 1 and patched 2 redundancy has a key role in regulating epidermal differentiation.

The following Ptch2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptch2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68509	NM_001312903.1 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu68512	XM_011240472.1 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu68512	XM_011240473.1 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu68513	XM_011240476.2 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu03183	NM_008958.3 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
OMu68510	XM_011240470.2 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu68511	XM_011240471.2 Latest version!	Mus musculus patched 2 (Ptch2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptch2 ( NM_001312903.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001299832.1 Mus musculus patched 2 (Ptch2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68509
Clone ID Related Accession (Same CDS sequence)	NM_001312903.1
Accession Version	NM_001312903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ056518.1, BC058397.1 and AK042653.1. On Aug 22, 2015 this sequence version replaced XM_011240475.1. Transcript Variant: This variant (2) uses an alternate splice site in its 5' UTR, resulting in the use of a downstream translation start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC058397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCCCC GTCAGCTGTA CGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGCGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCGGC CAACGCGTCC CAGCAGATCC ATGCCTTCTC CTCCACCACC 
CTGGATGACA TCCTGCGCGC GTTCTCTGAA GTCAGCACCA CCCGTGTGGT AGGAGGCTAT 
CTGCTTATGC TGGCCTATGC CTGCGTAACA ATGCTGCGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTAG GTCTTGCTGG GGTGTTGCTG GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CACAGGTGCT GCCCTTCTTG 
GCTCTGGGCA TTGGCGTGGA TGACATCTTC CTGCTGGCGC ATGCCTTCAC AAAAGCCCCA 
CCTGACACCC CTCTCCCAGA GCGTATGGGT GAGTGTTTGA GGAGCACGGG CACCAGCGTG 
GCACTCACAT CCGTCAACAA CATGGTCGCC TTTTTCATGG CTGCCCTGGT TCCCATTCCT 
GCGCTGCGGG CCTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTCGTCT TCCCTGCCAT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAACGCCTG 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCCGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAGCTAGGGG ACAGAGCAGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC CGTGCAAGCC 
TTCACCCACT GCGAAGCCAG CAGCCAGCAT GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAGCTTCTGA CCCACTGGGC TCCGAACTCT ATAGCCCTGG AGGGTCTACA 
CGGGACCTTC TCAGTCAGGA GGAGGGGACA GGGCCACAGG CGGCCTGCAG GCCCCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCACCTTT ACTGCTCCAG 
ACACGAGCCA AGGCCCTGGT GTTGCTGTTC TTTGGGGCTC TTTTGGGCCT GAGCCTCTAT 
GGAGCCACCT TGGTACAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTAG GGGCACCAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCTCTAGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGCTCCC TCAAGGCTGT GCTGCCCCCA CCTGCCACCC AGGCACCCCG CACCTGGCTT 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGGCTGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCTGCCACTC TTACCGCAAC GGCTCAGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAG CCTCTGGATT TCAGCCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAGCTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTCGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCAGAGTGGC TTCACGACAA ATATGATACC ACCGGGGAGA ACCTTCGCAT CCCGGCAGCC 
CAGCCCTTGG AGTTTGCCCA GTTCCCCTTC CTGCTGCATG GACTCCAGAA GACTGCAGAC 
TTTGTAGAAG CCATCGAAGG GGCCAGGGCG GCATGCACAG AGGCAGGCCA GGCAGGGGTG 
CATGCCTACC CCAGTGGCTC CCCCTTCCTC TTCTGGGAGC AGTATCTGGG TCTTCGGCGC 
TGCTTCCTGC TGGCAGTCTG CATCTTGCTG GTGTGCACCT TCCTCGTCTG TGCCCTGCTT 
CTACTCAGCC CGTGGACAGC TGGCCTCATA GTGCTGGTCC TGGCAATGAT GACTGTGGAG 
CTCTTTGGTA TCATGGGATT CCTGGGCATC AAACTGAGTG CCATCCCCGT GGTAATCCTC 
GTGGCCTCTA TAGGCATTGG TGTTGAATTC ACAGTTCACG TGGCTCTGGG CTTCCTGACC 
AGCCATGGTA GCCGGAACCT GCGGGCTGCT AGCGCTCTGG AACAGACCTT TGCCCCTGTG 
ACTGATGGAG CTGTCTCCAC CTTGCTGGGT CTGCTCATGC TTGCTGGTTC TAACTTTGAC 
TTCATCATAA GGTATTTCTT TGTGGTGCTG ACGGTGCTGA CACTCTTGGG CCTGCTCCAT 
GGACTCCTGC TGCTGCCTGT GCTGCTCTCT ATCCTAGGCC CCCCGCCACA GGTGGTGCAG 
GTATACAAGG AGAGTCCACA GACCCTAAAC TCCGCTGCTC CACAGAGAGG TGGGCTCAGG 
TGGGACAGGC CCCCCACCCT GCCCCAGAGT TTTGCCAGAG TGACTACCTC CATGACTGTG 
GCCCTCCACC CACCACCTCT GCCTGGAGCA TACGTCCACC CAGCCTCCGA GGAGCCCACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299832.1
CDS	1262..3844
Translation	MSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT

Target ORF information:

RefSeq Version	NM_001312903.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312903.1

ORF Insert Sequence:

ATGAGTCCCC GTCAGCTGTA CGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGCGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCGGC CAACGCGTCC CAGCAGATCC ATGCCTTCTC CTCCACCACC 
CTGGATGACA TCCTGCGCGC GTTCTCTGAA GTCAGCACCA CCCGTGTGGT AGGAGGCTAT 
CTGCTTATGC TGGCCTATGC CTGCGTAACA ATGCTGCGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTAG GTCTTGCTGG GGTGTTGCTG GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CACAGGTGCT GCCCTTCTTG 
GCTCTGGGCA TTGGCGTGGA TGACATCTTC CTGCTGGCGC ATGCCTTCAC AAAAGCCCCA 
CCTGACACCC CTCTCCCAGA GCGTATGGGT GAGTGTTTGA GGAGCACGGG CACCAGCGTG 
GCACTCACAT CCGTCAACAA CATGGTCGCC TTTTTCATGG CTGCCCTGGT TCCCATTCCT 
GCGCTGCGGG CCTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTCGTCT TCCCTGCCAT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAACGCCTG 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCCGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAGCTAGGGG ACAGAGCAGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC CGTGCAAGCC 
TTCACCCACT GCGAAGCCAG CAGCCAGCAT GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAGCTTCTGA CCCACTGGGC TCCGAACTCT ATAGCCCTGG AGGGTCTACA 
CGGGACCTTC TCAGTCAGGA GGAGGGGACA GGGCCACAGG CGGCCTGCAG GCCCCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCACCTTT ACTGCTCCAG 
ACACGAGCCA AGGCCCTGGT GTTGCTGTTC TTTGGGGCTC TTTTGGGCCT GAGCCTCTAT 
GGAGCCACCT TGGTACAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTAG GGGCACCAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCTCTAGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGCTCCC TCAAGGCTGT GCTGCCCCCA CCTGCCACCC AGGCACCCCG CACCTGGCTT 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGGCTGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCTGCCACTC TTACCGCAAC GGCTCAGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAG CCTCTGGATT TCAGCCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAGCTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTCGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCAGAGTGGC TTCACGACAA ATATGATACC ACCGGGGAGA ACCTTCGCAT CCCGGCAGCC 
CAGCCCTTGG AGTTTGCCCA GTTCCCCTTC CTGCTGCATG GACTCCAGAA GACTGCAGAC 
TTTGTAGAAG CCATCGAAGG GGCCAGGGCG GCATGCACAG AGGCAGGCCA GGCAGGGGTG 
CATGCCTACC CCAGTGGCTC CCCCTTCCTC TTCTGGGAGC AGTATCTGGG TCTTCGGCGC 
TGCTTCCTGC TGGCAGTCTG CATCTTGCTG GTGTGCACCT TCCTCGTCTG TGCCCTGCTT 
CTACTCAGCC CGTGGACAGC TGGCCTCATA GTGCTGGTCC TGGCAATGAT GACTGTGGAG 
CTCTTTGGTA TCATGGGATT CCTGGGCATC AAACTGAGTG CCATCCCCGT GGTAATCCTC 
GTGGCCTCTA TAGGCATTGG TGTTGAATTC ACAGTTCACG TGGCTCTGGG CTTCCTGACC 
AGCCATGGTA GCCGGAACCT GCGGGCTGCT AGCGCTCTGG AACAGACCTT TGCCCCTGTG 
ACTGATGGAG CTGTCTCCAC CTTGCTGGGT CTGCTCATGC TTGCTGGTTC TAACTTTGAC 
TTCATCATAA GGTATTTCTT TGTGGTGCTG ACGGTGCTGA CACTCTTGGG CCTGCTCCAT 
GGACTCCTGC TGCTGCCTGT GCTGCTCTCT ATCCTAGGCC CCCCGCCACA GGTGGTGCAG 
GTATACAAGG AGAGTCCACA GACCCTAAAC TCCGCTGCTC CACAGAGAGG TGGGCTCAGG 
TGGGACAGGC CCCCCACCCT GCCCCAGAGT TTTGCCAGAG TGACTACCTC CATGACTGTG 
GCCCTCCACC CACCACCTCT GCCTGGAGCA TACGTCCACC CAGCCTCCGA GGAGCCCACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_011240472.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238774.1 Mus musculus patched 2 (Ptch2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu68512
Clone ID Related Accession (Same CDS sequence)	XM_011240472.1 , XM_011240473.1
Accession Version	XM_011240472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCCC GTCAGCTGTA CGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGCGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCGGC CAACGCGTCC CAGCAGATCC ATGCCTTCTC CTCCACCACC 
CTGGATGACA TCCTGCGCGC GTTCTCTGAA GTCAGCACCA CCCGTGTGGT AGGAGGCTAT 
CTGCTTATGC TGGCCTATGC CTGCGTAACA ATGCTGCGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTAG GTCTTGCTGG GGTGTTGCTG GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CACAGGTGCT GCCCTTCTTG 
GCTCTGGGCA TTGGCGTGGA TGACATCTTC CTGCTGGCGC ATGCCTTCAC AAAAGCCCCA 
CCTGACACCC CTCTCCCAGA GCGTATGGGT GAGTGTTTGA GGAGCACGGG CACCAGCGTG 
GCACTCACAT CCGTCAACAA CATGGTCGCC TTTTTCATGG CTGCCCTGGT TCCCATTCCT 
GCGCTGCGGG CCTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTCGTCT TCCCTGCCAT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAACGCCTG 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCCGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAGCTAGGGG ACAGAGCAGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC CGTGCAAGCC 
TTCACCCACT GCGAAGCCAG CAGCCAGCAT GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAGCTTCTGA CCCACTGGGC TCCGAACTCT ATAGCCCTGG AGGGTCTACA 
CGGGACCTTC TCAGTCAGGA GGAGGGGACA GGGCCACAGG CGGCCTGCAG GCCCCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCACCTTT ACTGCTCCAG 
ACACGAGCCA AGGCCCTGGT GTTGCTGTTC TTTGGGGCTC TTTTGGGCCT GAGCCTCTAT 
GGAGCCACCT TGGTACAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTAG GGGCACCAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCTCTAGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGCTCCC TCAAGGCTGT GCTGCCCCCA CCTGCCACCC AGGCACCCCG CACCTGGCTT 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGGCTGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCTGCCACTC TTACCGCAAC GGCTCAGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAG CCTCTGGATT TCAGCCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAGCTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTCGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCAGAGTGGC TTCACGACAA ATATGATACC ACCGGGGAGA ACCTTCGCAG TGAGTCCCAT 
GGGAGCTCGC CCGGAACCTG GTCCTCAGCC CACACAGGCC CTGACCCTCC AACCCCTCAC 
TGCGCCCACT CCATCTGGTC CCTCTGCTCC TCCATCCTCT CCACAGTCCC GGCAGCCCAG 
CCCTTGGAGT TTGCCCAGTT CCCCTTCCTG CTGCATGGAC TCCAGAAGAC TGCAGACTTT 
GTAGAAGCCA TCGAAGGGGC CAGGGCGGCA TGCACAGAGG CAGGCCAGGC AGGGGTGCAT 
GCCTACCCCA GTGGCTCCCC CTTCCTCTTC TGGGAGCAGT ATCTGGGTCT TCGGCGCTGC 
TTCCTGCTGG CAGTCTGCAT CTTGCTGGTG TGCACCTTCC TCGTCTGTGC CCTGCTTCTA 
CTCAGCCCGT GGACAGCTGG CCTCATAGTG CTGGTCCTGG CAATGATGAC TGTGGAGCTC 
TTTGGTATCA TGGGATTCCT GGGCATCAAA CTGAGTGCCA TCCCCGTGGT AATCCTCGTG 
GCCTCTATAG GCATTGGTGT TGAATTCACA GTTCACGTGG CTCTGGGCTT CCTGACCAGC 
CATGGTAGCC GGAACCTGCG GGCTGCTAGC GCTCTGGAAC AGACCTTTGC CCCTGTGACT 
GATGGAGCTG TCTCCACCTT GCTGGGTCTG CTCATGCTTG CTGGTTCTAA CTTTGACTTC 
ATCATAAGGT ATTTCTTTGT GGTGCTGACG GTGCTGACAC TCTTGGGCCT GCTCCATGGA 
CTCCTGCTGC TGCCTGTGCT GCTCTCTATC CTAGGCCCCC CGCCACAGGT GGTGCAGGTA 
TACAAGGAGA GTCCACAGAC CCTAAACTCC GCTGCTCCAC AGAGAGGTGG GCTCAGGTGG 
GACAGGCCCC CCACCCTGCC CCAGAGTTTT GCCAGAGTGA CTACCTCCAT GACTGTGGCC 
CTCCACCCAC CACCTCTGCC TGGAGCATAC GTCCACCCAG CCTCCGAGGA GCCCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238774.1
CDS	460..3159
Translation	MSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRSESHGSSPGTWSSAHTGPDPPTPHCAHSIWSLCSSILSTVPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT

Target ORF information:

RefSeq Version	XM_011240472.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240472.1

ORF Insert Sequence:

ATGAGTCCCC GTCAGCTGTA CGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGCGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCGGC CAACGCGTCC CAGCAGATCC ATGCCTTCTC CTCCACCACC 
CTGGATGACA TCCTGCGCGC GTTCTCTGAA GTCAGCACCA CCCGTGTGGT AGGAGGCTAT 
CTGCTTATGC TGGCCTATGC CTGCGTAACA ATGCTGCGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTAG GTCTTGCTGG GGTGTTGCTG GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CACAGGTGCT GCCCTTCTTG 
GCTCTGGGCA TTGGCGTGGA TGACATCTTC CTGCTGGCGC ATGCCTTCAC AAAAGCCCCA 
CCTGACACCC CTCTCCCAGA GCGTATGGGT GAGTGTTTGA GGAGCACGGG CACCAGCGTG 
GCACTCACAT CCGTCAACAA CATGGTCGCC TTTTTCATGG CTGCCCTGGT TCCCATTCCT 
GCGCTGCGGG CCTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTCGTCT TCCCTGCCAT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAACGCCTG 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCCGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAGCTAGGGG ACAGAGCAGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC CGTGCAAGCC 
TTCACCCACT GCGAAGCCAG CAGCCAGCAT GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAGCTTCTGA CCCACTGGGC TCCGAACTCT ATAGCCCTGG AGGGTCTACA 
CGGGACCTTC TCAGTCAGGA GGAGGGGACA GGGCCACAGG CGGCCTGCAG GCCCCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCACCTTT ACTGCTCCAG 
ACACGAGCCA AGGCCCTGGT GTTGCTGTTC TTTGGGGCTC TTTTGGGCCT GAGCCTCTAT 
GGAGCCACCT TGGTACAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTAG GGGCACCAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCTCTAGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGCTCCC TCAAGGCTGT GCTGCCCCCA CCTGCCACCC AGGCACCCCG CACCTGGCTT 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGGCTGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCTGCCACTC TTACCGCAAC GGCTCAGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAG CCTCTGGATT TCAGCCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAGCTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTCGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCAGAGTGGC TTCACGACAA ATATGATACC ACCGGGGAGA ACCTTCGCAG TGAGTCCCAT 
GGGAGCTCGC CCGGAACCTG GTCCTCAGCC CACACAGGCC CTGACCCTCC AACCCCTCAC 
TGCGCCCACT CCATCTGGTC CCTCTGCTCC TCCATCCTCT CCACAGTCCC GGCAGCCCAG 
CCCTTGGAGT TTGCCCAGTT CCCCTTCCTG CTGCATGGAC TCCAGAAGAC TGCAGACTTT 
GTAGAAGCCA TCGAAGGGGC CAGGGCGGCA TGCACAGAGG CAGGCCAGGC AGGGGTGCAT 
GCCTACCCCA GTGGCTCCCC CTTCCTCTTC TGGGAGCAGT ATCTGGGTCT TCGGCGCTGC 
TTCCTGCTGG CAGTCTGCAT CTTGCTGGTG TGCACCTTCC TCGTCTGTGC CCTGCTTCTA 
CTCAGCCCGT GGACAGCTGG CCTCATAGTG CTGGTCCTGG CAATGATGAC TGTGGAGCTC 
TTTGGTATCA TGGGATTCCT GGGCATCAAA CTGAGTGCCA TCCCCGTGGT AATCCTCGTG 
GCCTCTATAG GCATTGGTGT TGAATTCACA GTTCACGTGG CTCTGGGCTT CCTGACCAGC 
CATGGTAGCC GGAACCTGCG GGCTGCTAGC GCTCTGGAAC AGACCTTTGC CCCTGTGACT 
GATGGAGCTG TCTCCACCTT GCTGGGTCTG CTCATGCTTG CTGGTTCTAA CTTTGACTTC 
ATCATAAGGT ATTTCTTTGT GGTGCTGACG GTGCTGACAC TCTTGGGCCT GCTCCATGGA 
CTCCTGCTGC TGCCTGTGCT GCTCTCTATC CTAGGCCCCC CGCCACAGGT GGTGCAGGTA 
TACAAGGAGA GTCCACAGAC CCTAAACTCC GCTGCTCCAC AGAGAGGTGG GCTCAGGTGG 
GACAGGCCCC CCACCCTGCC CCAGAGTTTT GCCAGAGTGA CTACCTCCAT GACTGTGGCC 
CTCCACCCAC CACCTCTGCC TGGAGCATAC GTCCACCCAG CCTCCGAGGA GCCCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_011240473.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238775.1 Mus musculus patched 2 (Ptch2), transcript variant X4, mRNA.

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CloneID	OMu68512
Clone ID Related Accession (Same CDS sequence)	XM_011240472.1 , XM_011240473.1
Accession Version	XM_011240473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCCC GTCAGCTGTA CGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGCGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCGGC CAACGCGTCC CAGCAGATCC ATGCCTTCTC CTCCACCACC 
CTGGATGACA TCCTGCGCGC GTTCTCTGAA GTCAGCACCA CCCGTGTGGT AGGAGGCTAT 
CTGCTTATGC TGGCCTATGC CTGCGTAACA ATGCTGCGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTAG GTCTTGCTGG GGTGTTGCTG GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CACAGGTGCT GCCCTTCTTG 
GCTCTGGGCA TTGGCGTGGA TGACATCTTC CTGCTGGCGC ATGCCTTCAC AAAAGCCCCA 
CCTGACACCC CTCTCCCAGA GCGTATGGGT GAGTGTTTGA GGAGCACGGG CACCAGCGTG 
GCACTCACAT CCGTCAACAA CATGGTCGCC TTTTTCATGG CTGCCCTGGT TCCCATTCCT 
GCGCTGCGGG CCTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTCGTCT TCCCTGCCAT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAACGCCTG 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCCGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAGCTAGGGG ACAGAGCAGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC CGTGCAAGCC 
TTCACCCACT GCGAAGCCAG CAGCCAGCAT GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAGCTTCTGA CCCACTGGGC TCCGAACTCT ATAGCCCTGG AGGGTCTACA 
CGGGACCTTC TCAGTCAGGA GGAGGGGACA GGGCCACAGG CGGCCTGCAG GCCCCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCACCTTT ACTGCTCCAG 
ACACGAGCCA AGGCCCTGGT GTTGCTGTTC TTTGGGGCTC TTTTGGGCCT GAGCCTCTAT 
GGAGCCACCT TGGTACAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTAG GGGCACCAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCTCTAGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGCTCCC TCAAGGCTGT GCTGCCCCCA CCTGCCACCC AGGCACCCCG CACCTGGCTT 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGGCTGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCTGCCACTC TTACCGCAAC GGCTCAGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAG CCTCTGGATT TCAGCCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAGCTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTCGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCAGAGTGGC TTCACGACAA ATATGATACC ACCGGGGAGA ACCTTCGCAG TGAGTCCCAT 
GGGAGCTCGC CCGGAACCTG GTCCTCAGCC CACACAGGCC CTGACCCTCC AACCCCTCAC 
TGCGCCCACT CCATCTGGTC CCTCTGCTCC TCCATCCTCT CCACAGTCCC GGCAGCCCAG 
CCCTTGGAGT TTGCCCAGTT CCCCTTCCTG CTGCATGGAC TCCAGAAGAC TGCAGACTTT 
GTAGAAGCCA TCGAAGGGGC CAGGGCGGCA TGCACAGAGG CAGGCCAGGC AGGGGTGCAT 
GCCTACCCCA GTGGCTCCCC CTTCCTCTTC TGGGAGCAGT ATCTGGGTCT TCGGCGCTGC 
TTCCTGCTGG CAGTCTGCAT CTTGCTGGTG TGCACCTTCC TCGTCTGTGC CCTGCTTCTA 
CTCAGCCCGT GGACAGCTGG CCTCATAGTG CTGGTCCTGG CAATGATGAC TGTGGAGCTC 
TTTGGTATCA TGGGATTCCT GGGCATCAAA CTGAGTGCCA TCCCCGTGGT AATCCTCGTG 
GCCTCTATAG GCATTGGTGT TGAATTCACA GTTCACGTGG CTCTGGGCTT CCTGACCAGC 
CATGGTAGCC GGAACCTGCG GGCTGCTAGC GCTCTGGAAC AGACCTTTGC CCCTGTGACT 
GATGGAGCTG TCTCCACCTT GCTGGGTCTG CTCATGCTTG CTGGTTCTAA CTTTGACTTC 
ATCATAAGGT ATTTCTTTGT GGTGCTGACG GTGCTGACAC TCTTGGGCCT GCTCCATGGA 
CTCCTGCTGC TGCCTGTGCT GCTCTCTATC CTAGGCCCCC CGCCACAGGT GGTGCAGGTA 
TACAAGGAGA GTCCACAGAC CCTAAACTCC GCTGCTCCAC AGAGAGGTGG GCTCAGGTGG 
GACAGGCCCC CCACCCTGCC CCAGAGTTTT GCCAGAGTGA CTACCTCCAT GACTGTGGCC 
CTCCACCCAC CACCTCTGCC TGGAGCATAC GTCCACCCAG CCTCCGAGGA GCCCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238775.1
CDS	119..2818
Translation	MSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRSESHGSSPGTWSSAHTGPDPPTPHCAHSIWSLCSSILSTVPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT

Target ORF information:

RefSeq Version	XM_011240473.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240473.1

ORF Insert Sequence:

ATGAGTCCCC GTCAGCTGTA CGAGCACTTC CGGGGCGACT ACCAGACACA TGACATCGGC 
TGGAGCGAGG AGCAGGCCAG CATGGTGCTG CAGGCCTGGC AGAGGCGCTT TGTGCAGCTA 
GCCCAGGAGG CTCTGCCGGC CAACGCGTCC CAGCAGATCC ATGCCTTCTC CTCCACCACC 
CTGGATGACA TCCTGCGCGC GTTCTCTGAA GTCAGCACCA CCCGTGTGGT AGGAGGCTAT 
CTGCTTATGC TGGCCTATGC CTGCGTAACA ATGCTGCGGT GGGACTGCGC CCAGTCCCAG 
GGTGCCGTAG GTCTTGCTGG GGTGTTGCTG GTGGCCCTGG CGGTGGCCTC AGGTCTTGGG 
CTTTGTGCCC TACTTGGCAT CACTTTCAAT GCTGCCACTA CACAGGTGCT GCCCTTCTTG 
GCTCTGGGCA TTGGCGTGGA TGACATCTTC CTGCTGGCGC ATGCCTTCAC AAAAGCCCCA 
CCTGACACCC CTCTCCCAGA GCGTATGGGT GAGTGTTTGA GGAGCACGGG CACCAGCGTG 
GCACTCACAT CCGTCAACAA CATGGTCGCC TTTTTCATGG CTGCCCTGGT TCCCATTCCT 
GCGCTGCGGG CCTTCTCCCT GCAGGCAGCC ATAGTGGTCG GCTGCAACTT TGCAGCGGTA 
ATGCTCGTCT TCCCTGCCAT CCTCAGCCTC GACCTGCGCC GGCGCCACCG CCAACGCCTG 
GACGTTCTCT GCTGCTTTTC TAGCCCCTGC TCCGCTCAGG TCATTCAGAT GCTGCCCCAG 
GAGCTAGGGG ACAGAGCAGT ACCAGTGGGC ATTGCCCACC TGACTGCCAC CGTGCAAGCC 
TTCACCCACT GCGAAGCCAG CAGCCAGCAT GTAGTCACCA TTTTGCCTCC TCAAGCCCAC 
CTGCTGTCTC CAGCTTCTGA CCCACTGGGC TCCGAACTCT ATAGCCCTGG AGGGTCTACA 
CGGGACCTTC TCAGTCAGGA GGAGGGGACA GGGCCACAGG CGGCCTGCAG GCCCCTGCTC 
TGTGCCCACT GGACTCTCGC CCATTTTGCC CGCTATCAGT TTGCACCTTT ACTGCTCCAG 
ACACGAGCCA AGGCCCTGGT GTTGCTGTTC TTTGGGGCTC TTTTGGGCCT GAGCCTCTAT 
GGAGCCACCT TGGTACAAGA TGGGCTGGCC CTGACAGATG TGGTCCCTAG GGGCACCAAG 
GAACATGCTT TCCTGAGCGC CCAGCTCAGG TACTTCTCCC TGTACGAGGT GGCTCTAGTG 
ACACAGGGTG GCTTTGACTA CGCCCACTCC CAACGCGCCC TCTTTGATCT GCACCAGCGC 
TTCAGCTCCC TCAAGGCTGT GCTGCCCCCA CCTGCCACCC AGGCACCCCG CACCTGGCTT 
CACTACTACC GCAGCTGGCT ACAGGGTATC CAGGCTGCAT TTGACCAAGA CTGGGCTTCT 
GGGCGCATCA CCTGCCACTC TTACCGCAAC GGCTCAGAAG ATGGCGCCCT GGCCTACAAG 
CTGCTCATCC AAACCGGGAA TGCCCAGGAG CCTCTGGATT TCAGCCAGCT GACCACAAGG 
AAACTGGTGG ACAAGGAGGG ACTCATTCCC CCAGAGCTCT TCTACATGGG GCTAACCGTG 
TGGGTGAGCA GCGACCCCCT GGGCCTCGCA GCCTCTCAGG CCAACTTCTA CCCCCCACCT 
CCAGAGTGGC TTCACGACAA ATATGATACC ACCGGGGAGA ACCTTCGCAG TGAGTCCCAT 
GGGAGCTCGC CCGGAACCTG GTCCTCAGCC CACACAGGCC CTGACCCTCC AACCCCTCAC 
TGCGCCCACT CCATCTGGTC CCTCTGCTCC TCCATCCTCT CCACAGTCCC GGCAGCCCAG 
CCCTTGGAGT TTGCCCAGTT CCCCTTCCTG CTGCATGGAC TCCAGAAGAC TGCAGACTTT 
GTAGAAGCCA TCGAAGGGGC CAGGGCGGCA TGCACAGAGG CAGGCCAGGC AGGGGTGCAT 
GCCTACCCCA GTGGCTCCCC CTTCCTCTTC TGGGAGCAGT ATCTGGGTCT TCGGCGCTGC 
TTCCTGCTGG CAGTCTGCAT CTTGCTGGTG TGCACCTTCC TCGTCTGTGC CCTGCTTCTA 
CTCAGCCCGT GGACAGCTGG CCTCATAGTG CTGGTCCTGG CAATGATGAC TGTGGAGCTC 
TTTGGTATCA TGGGATTCCT GGGCATCAAA CTGAGTGCCA TCCCCGTGGT AATCCTCGTG 
GCCTCTATAG GCATTGGTGT TGAATTCACA GTTCACGTGG CTCTGGGCTT CCTGACCAGC 
CATGGTAGCC GGAACCTGCG GGCTGCTAGC GCTCTGGAAC AGACCTTTGC CCCTGTGACT 
GATGGAGCTG TCTCCACCTT GCTGGGTCTG CTCATGCTTG CTGGTTCTAA CTTTGACTTC 
ATCATAAGGT ATTTCTTTGT GGTGCTGACG GTGCTGACAC TCTTGGGCCT GCTCCATGGA 
CTCCTGCTGC TGCCTGTGCT GCTCTCTATC CTAGGCCCCC CGCCACAGGT GGTGCAGGTA 
TACAAGGAGA GTCCACAGAC CCTAAACTCC GCTGCTCCAC AGAGAGGTGG GCTCAGGTGG 
GACAGGCCCC CCACCCTGCC CCAGAGTTTT GCCAGAGTGA CTACCTCCAT GACTGTGGCC 
CTCCACCCAC CACCTCTGCC TGGAGCATAC GTCCACCCAG CCTCCGAGGA GCCCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_011240476.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238778.1 Mus musculus patched 2 (Ptch2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu68513
Clone ID Related Accession (Same CDS sequence)	XM_011240476.2
Accession Version	XM_011240476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGGTAGGA 
GGCTATCTGC TTATGCTGGC CTATGCCTGC GTAACAATGC TGCGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTAGGTCT TGCTGGGGTG TTGCTGGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACACA GGTGCTGCCC 
TTCTTGGCTC TGGGCATTGG CGTGGATGAC ATCTTCCTGC TGGCGCATGC CTTCACAAAA 
GCCCCACCTG ACACCCCTCT CCCAGAGCGT ATGGGTGAGT GTTTGAGGAG CACGGGCACC 
AGCGTGGCAC TCACATCCGT CAACAACATG GTCGCCTTTT TCATGGCTGC CCTGGTTCCC 
ATTCCTGCGC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TCGTCTTCCC TGCCATCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAA 
CGCCTGGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCCG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAGC TAGGGGACAG AGCAGTACCA GTGGGCATTG CCCACCTGAC TGCCACCGTG 
CAAGCCTTCA CCCACTGCGA AGCCAGCAGC CAGCATGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAGC TTCTGACCCA CTGGGCTCCG AACTCTATAG CCCTGGAGGG 
TCTACACGGG ACCTTCTCAG TCAGGAGGAG GGGACAGGGC CACAGGCGGC CTGCAGGCCC 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC ACCTTTACTG 
CTCCAGACAC GAGCCAAGGC CCTGGTGTTG CTGTTCTTTG GGGCTCTTTT GGGCCTGAGC 
CTCTATGGAG CCACCTTGGT ACAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTAGGGGC 
ACCAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGCGCTT CAGCTCCCTC AAGGCTGTGC 
TGCCCCCACC TGCCACCCAG GCACCCCGCA CCTGGCTTCA CTACTACCGC AGCTGGCTAC 
AGGGTATCCA GGCTGCATTT GACCAAGACT GGGCTTCTGG GCGCATCACC TGCCACTCTT 
ACCGCAACGG CTCAGAAGAT GGCGCCCTGG CCTACAAGCT GCTCATCCAA ACCGGGAATG 
CCCAGGAGCC TCTGGATTTC AGCCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238778.1
CDS	577..3006
Translation	MVRPLSLGELPPSYTPPARSSAPHILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVAFGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTAHQEGGNVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLSASAPSRLCCPHLPPRHPAPGFTTTAAGYRVSRLHLTKTGLLGASPATLTATAQKMAPWPTSCSSKPGMPRSLWISAS

Target ORF information:

RefSeq Version	XM_011240476.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240476.2

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGGTAGGA 
GGCTATCTGC TTATGCTGGC CTATGCCTGC GTAACAATGC TGCGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTAGGTCT TGCTGGGGTG TTGCTGGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACACA GGTGCTGCCC 
TTCTTGGCTC TGGGCATTGG CGTGGATGAC ATCTTCCTGC TGGCGCATGC CTTCACAAAA 
GCCCCACCTG ACACCCCTCT CCCAGAGCGT ATGGGTGAGT GTTTGAGGAG CACGGGCACC 
AGCGTGGCAC TCACATCCGT CAACAACATG GTCGCCTTTT TCATGGCTGC CCTGGTTCCC 
ATTCCTGCGC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TCGTCTTCCC TGCCATCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAA 
CGCCTGGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCCG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAGC TAGGGGACAG AGCAGTACCA GTGGGCATTG CCCACCTGAC TGCCACCGTG 
CAAGCCTTCA CCCACTGCGA AGCCAGCAGC CAGCATGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAGC TTCTGACCCA CTGGGCTCCG AACTCTATAG CCCTGGAGGG 
TCTACACGGG ACCTTCTCAG TCAGGAGGAG GGGACAGGGC CACAGGCGGC CTGCAGGCCC 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC ACCTTTACTG 
CTCCAGACAC GAGCCAAGGC CCTGGTGTTG CTGTTCTTTG GGGCTCTTTT GGGCCTGAGC 
CTCTATGGAG CCACCTTGGT ACAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTAGGGGC 
ACCAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGCGCTT CAGCTCCCTC AAGGCTGTGC 
TGCCCCCACC TGCCACCCAG GCACCCCGCA CCTGGCTTCA CTACTACCGC AGCTGGCTAC 
AGGGTATCCA GGCTGCATTT GACCAAGACT GGGCTTCTGG GCGCATCACC TGCCACTCTT 
ACCGCAACGG CTCAGAAGAT GGCGCCCTGG CCTACAAGCT GCTCATCCAA ACCGGGAATG 
CCCAGGAGCC TCTGGATTTC AGCCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( NM_008958.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032984.1 Mus musculus patched 2 (Ptch2), transcript variant 1, mRNA.

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CloneID	OMu03183
Clone ID Related Accession (Same CDS sequence)	NM_008958.3
Accession Version	NM_008958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ056518.1, BC058397.1, CF733501.1 and AK042653.1. On Aug 22, 2015 this sequence version replaced NM_008958.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB010833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGGTAGGA 
GGCTATCTGC TTATGCTGGC CTATGCCTGC GTAACAATGC TGCGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTAGGTCT TGCTGGGGTG TTGCTGGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACACA GGTGCTGCCC 
TTCTTGGCTC TGGGCATTGG CGTGGATGAC ATCTTCCTGC TGGCGCATGC CTTCACAAAA 
GCCCCACCTG ACACCCCTCT CCCAGAGCGT ATGGGTGAGT GTTTGAGGAG CACGGGCACC 
AGCGTGGCAC TCACATCCGT CAACAACATG GTCGCCTTTT TCATGGCTGC CCTGGTTCCC 
ATTCCTGCGC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TCGTCTTCCC TGCCATCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAA 
CGCCTGGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCCG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAGC TAGGGGACAG AGCAGTACCA GTGGGCATTG CCCACCTGAC TGCCACCGTG 
CAAGCCTTCA CCCACTGCGA AGCCAGCAGC CAGCATGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAGC TTCTGACCCA CTGGGCTCCG AACTCTATAG CCCTGGAGGG 
TCTACACGGG ACCTTCTCAG TCAGGAGGAG GGGACAGGGC CACAGGCGGC CTGCAGGCCC 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC ACCTTTACTG 
CTCCAGACAC GAGCCAAGGC CCTGGTGTTG CTGTTCTTTG GGGCTCTTTT GGGCCTGAGC 
CTCTATGGAG CCACCTTGGT ACAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTAGGGGC 
ACCAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCT 
CTAGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCCCTCAA GGCTGTGCTG CCCCCACCTG CCACCCAGGC ACCCCGCACC 
TGGCTTCACT ACTACCGCAG CTGGCTACAG GGTATCCAGG CTGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCTG CCACTCTTAC CGCAACGGCT CAGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAGCCTC TGGATTTCAG CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AGCTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTCGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCAG AGTGGCTTCA CGACAAATAT GATACCACCG GGGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGC TGCATGGACT CCAGAAGACT 
GCAGACTTTG TAGAAGCCAT CGAAGGGGCC AGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCATG CCTACCCCAG TGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGTCTT 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC TTGCTGGTGT GCACCTTCCT CGTCTGTGCC 
CTGCTTCTAC TCAGCCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC AATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTATAGG CATTGGTGTT GAATTCACAG TTCACGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTAGCG CTCTGGAACA GACCTTTGCC 
CCTGTGACTG ATGGAGCTGT CTCCACCTTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA TTTCTTTGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT GCCTGTGCTG CTCTCTATCC TAGGCCCCCC GCCACAGGTG 
GTGCAGGTAT ACAAGGAGAG TCCACAGACC CTAAACTCCG CTGCTCCACA GAGAGGTGGG 
CTCAGGTGGG ACAGGCCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCTCTGCCT GGAGCATACG TCCACCCAGC CTCCGAGGAG 
CCCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032984.1
CDS	301..3849
Translation	MVRPLSLGELPPSYTPPARSSAPHILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVAFGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTAHQEGGNVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT

Target ORF information:

RefSeq Version	NM_008958.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008958.3

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGGTAGGA 
GGCTATCTGC TTATGCTGGC CTATGCCTGC GTAACAATGC TGCGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTAGGTCT TGCTGGGGTG TTGCTGGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACACA GGTGCTGCCC 
TTCTTGGCTC TGGGCATTGG CGTGGATGAC ATCTTCCTGC TGGCGCATGC CTTCACAAAA 
GCCCCACCTG ACACCCCTCT CCCAGAGCGT ATGGGTGAGT GTTTGAGGAG CACGGGCACC 
AGCGTGGCAC TCACATCCGT CAACAACATG GTCGCCTTTT TCATGGCTGC CCTGGTTCCC 
ATTCCTGCGC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TCGTCTTCCC TGCCATCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAA 
CGCCTGGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCCG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAGC TAGGGGACAG AGCAGTACCA GTGGGCATTG CCCACCTGAC TGCCACCGTG 
CAAGCCTTCA CCCACTGCGA AGCCAGCAGC CAGCATGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAGC TTCTGACCCA CTGGGCTCCG AACTCTATAG CCCTGGAGGG 
TCTACACGGG ACCTTCTCAG TCAGGAGGAG GGGACAGGGC CACAGGCGGC CTGCAGGCCC 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC ACCTTTACTG 
CTCCAGACAC GAGCCAAGGC CCTGGTGTTG CTGTTCTTTG GGGCTCTTTT GGGCCTGAGC 
CTCTATGGAG CCACCTTGGT ACAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTAGGGGC 
ACCAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCT 
CTAGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCCCTCAA GGCTGTGCTG CCCCCACCTG CCACCCAGGC ACCCCGCACC 
TGGCTTCACT ACTACCGCAG CTGGCTACAG GGTATCCAGG CTGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCTG CCACTCTTAC CGCAACGGCT CAGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAGCCTC TGGATTTCAG CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AGCTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTCGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCAG AGTGGCTTCA CGACAAATAT GATACCACCG GGGAGAACCT TCGCATCCCG 
GCAGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTGC TGCATGGACT CCAGAAGACT 
GCAGACTTTG TAGAAGCCAT CGAAGGGGCC AGGGCGGCAT GCACAGAGGC AGGCCAGGCA 
GGGGTGCATG CCTACCCCAG TGGCTCCCCC TTCCTCTTCT GGGAGCAGTA TCTGGGTCTT 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC TTGCTGGTGT GCACCTTCCT CGTCTGTGCC 
CTGCTTCTAC TCAGCCCGTG GACAGCTGGC CTCATAGTGC TGGTCCTGGC AATGATGACT 
GTGGAGCTCT TTGGTATCAT GGGATTCCTG GGCATCAAAC TGAGTGCCAT CCCCGTGGTA 
ATCCTCGTGG CCTCTATAGG CATTGGTGTT GAATTCACAG TTCACGTGGC TCTGGGCTTC 
CTGACCAGCC ATGGTAGCCG GAACCTGCGG GCTGCTAGCG CTCTGGAACA GACCTTTGCC 
CCTGTGACTG ATGGAGCTGT CTCCACCTTG CTGGGTCTGC TCATGCTTGC TGGTTCTAAC 
TTTGACTTCA TCATAAGGTA TTTCTTTGTG GTGCTGACGG TGCTGACACT CTTGGGCCTG 
CTCCATGGAC TCCTGCTGCT GCCTGTGCTG CTCTCTATCC TAGGCCCCCC GCCACAGGTG 
GTGCAGGTAT ACAAGGAGAG TCCACAGACC CTAAACTCCG CTGCTCCACA GAGAGGTGGG 
CTCAGGTGGG ACAGGCCCCC CACCCTGCCC CAGAGTTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC ACCTCTGCCT GGAGCATACG TCCACCCAGC CTCCGAGGAG 
CCCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_011240470.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238772.1 Mus musculus patched 2 (Ptch2), transcript variant X1, mRNA.

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CloneID	OMu68510
Clone ID Related Accession (Same CDS sequence)	XM_011240470.2
Accession Version	XM_011240470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGGTAGGA 
GGCTATCTGC TTATGCTGGC CTATGCCTGC GTAACAATGC TGCGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTAGGTCT TGCTGGGGTG TTGCTGGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACACA GGTGCTGCCC 
TTCTTGGCTC TGGGCATTGG CGTGGATGAC ATCTTCCTGC TGGCGCATGC CTTCACAAAA 
GCCCCACCTG ACACCCCTCT CCCAGAGCGT ATGGGTGAGT GTTTGAGGAG CACGGGCACC 
AGCGTGGCAC TCACATCCGT CAACAACATG GTCGCCTTTT TCATGGCTGC CCTGGTTCCC 
ATTCCTGCGC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TCGTCTTCCC TGCCATCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAA 
CGCCTGGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCCG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAGC TAGGGGACAG AGCAGTACCA GTGGGCATTG CCCACCTGAC TGCCACCGTG 
CAAGCCTTCA CCCACTGCGA AGCCAGCAGC CAGCATGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAGC TTCTGACCCA CTGGGCTCCG AACTCTATAG CCCTGGAGGG 
TCTACACGGG ACCTTCTCAG TCAGGAGGAG GGGACAGGGC CACAGGCGGC CTGCAGGCCC 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC ACCTTTACTG 
CTCCAGACAC GAGCCAAGGC CCTGGTGTTG CTGTTCTTTG GGGCTCTTTT GGGCCTGAGC 
CTCTATGGAG CCACCTTGGT ACAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTAGGGGC 
ACCAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCT 
CTAGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCCCTCAA GGCTGTGCTG CCCCCACCTG CCACCCAGGC ACCCCGCACC 
TGGCTTCACT ACTACCGCAG CTGGCTACAG GGTATCCAGG CTGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCTG CCACTCTTAC CGCAACGGCT CAGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAGCCTC TGGATTTCAG CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AGCTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTCGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCAG AGTGGCTTCA CGACAAATAT GATACCACCG GGGAGAACCT TCGCAGTGAG 
TCCCATGGGA GCTCGCCCGG AACCTGGTCC TCAGCCCACA CAGGCCCTGA CCCTCCAACC 
CCTCACTGCG CCCACTCCAT CTGGTCCCTC TGCTCCTCCA TCCTCTCCAC AGTCCCGGCA 
GCCCAGCCCT TGGAGTTTGC CCAGTTCCCC TTCCTGCTGC ATGGACTCCA GAAGACTGCA 
GACTTTGTAG AAGCCATCGA AGGGGCCAGG GCGGCATGCA CAGAGGCAGG CCAGGCAGGG 
GTGCATGCCT ACCCCAGTGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGTCTTCGG 
CGCTGCTTCC TGCTGGCAGT CTGCATCTTG CTGGTGTGCA CCTTCCTCGT CTGTGCCCTG 
CTTCTACTCA GCCCGTGGAC AGCTGGCCTC ATAGTGCTGG TCCTGGCAAT GATGACTGTG 
GAGCTCTTTG GTATCATGGG ATTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTAATC 
CTCGTGGCCT CTATAGGCAT TGGTGTTGAA TTCACAGTTC ACGTGGCTCT GGGCTTCCTG 
ACCAGCCATG GTAGCCGGAA CCTGCGGGCT GCTAGCGCTC TGGAACAGAC CTTTGCCCCT 
GTGACTGATG GAGCTGTCTC CACCTTGCTG GGTCTGCTCA TGCTTGCTGG TTCTAACTTT 
GACTTCATCA TAAGGTATTT CTTTGTGGTG CTGACGGTGC TGACACTCTT GGGCCTGCTC 
CATGGACTCC TGCTGCTGCC TGTGCTGCTC TCTATCCTAG GCCCCCCGCC ACAGGTGGTG 
CAGGTATACA AGGAGAGTCC ACAGACCCTA AACTCCGCTG CTCCACAGAG AGGTGGGCTC 
AGGTGGGACA GGCCCCCCAC CCTGCCCCAG AGTTTTGCCA GAGTGACTAC CTCCATGACT 
GTGGCCCTCC ACCCACCACC TCTGCCTGGA GCATACGTCC ACCCAGCCTC CGAGGAGCCC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238772.1
CDS	571..4236
Translation	MVRPLSLGELPPSYTPPARSSAPHILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVAFGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTAHQEGGNVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRSESHGSSPGTWSSAHTGPDPPTPHCAHSIWSLCSSILSTVPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT

Target ORF information:

RefSeq Version	XM_011240470.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240470.2

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGGTAGGA 
GGCTATCTGC TTATGCTGGC CTATGCCTGC GTAACAATGC TGCGGTGGGA CTGCGCCCAG 
TCCCAGGGTG CCGTAGGTCT TGCTGGGGTG TTGCTGGTGG CCCTGGCGGT GGCCTCAGGT 
CTTGGGCTTT GTGCCCTACT TGGCATCACT TTCAATGCTG CCACTACACA GGTGCTGCCC 
TTCTTGGCTC TGGGCATTGG CGTGGATGAC ATCTTCCTGC TGGCGCATGC CTTCACAAAA 
GCCCCACCTG ACACCCCTCT CCCAGAGCGT ATGGGTGAGT GTTTGAGGAG CACGGGCACC 
AGCGTGGCAC TCACATCCGT CAACAACATG GTCGCCTTTT TCATGGCTGC CCTGGTTCCC 
ATTCCTGCGC TGCGGGCCTT CTCCCTGCAG GCAGCCATAG TGGTCGGCTG CAACTTTGCA 
GCGGTAATGC TCGTCTTCCC TGCCATCCTC AGCCTCGACC TGCGCCGGCG CCACCGCCAA 
CGCCTGGACG TTCTCTGCTG CTTTTCTAGC CCCTGCTCCG CTCAGGTCAT TCAGATGCTG 
CCCCAGGAGC TAGGGGACAG AGCAGTACCA GTGGGCATTG CCCACCTGAC TGCCACCGTG 
CAAGCCTTCA CCCACTGCGA AGCCAGCAGC CAGCATGTAG TCACCATTTT GCCTCCTCAA 
GCCCACCTGC TGTCTCCAGC TTCTGACCCA CTGGGCTCCG AACTCTATAG CCCTGGAGGG 
TCTACACGGG ACCTTCTCAG TCAGGAGGAG GGGACAGGGC CACAGGCGGC CTGCAGGCCC 
CTGCTCTGTG CCCACTGGAC TCTCGCCCAT TTTGCCCGCT ATCAGTTTGC ACCTTTACTG 
CTCCAGACAC GAGCCAAGGC CCTGGTGTTG CTGTTCTTTG GGGCTCTTTT GGGCCTGAGC 
CTCTATGGAG CCACCTTGGT ACAAGATGGG CTGGCCCTGA CAGATGTGGT CCCTAGGGGC 
ACCAAGGAAC ATGCTTTCCT GAGCGCCCAG CTCAGGTACT TCTCCCTGTA CGAGGTGGCT 
CTAGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GCTCCCTCAA GGCTGTGCTG CCCCCACCTG CCACCCAGGC ACCCCGCACC 
TGGCTTCACT ACTACCGCAG CTGGCTACAG GGTATCCAGG CTGCATTTGA CCAAGACTGG 
GCTTCTGGGC GCATCACCTG CCACTCTTAC CGCAACGGCT CAGAAGATGG CGCCCTGGCC 
TACAAGCTGC TCATCCAAAC CGGGAATGCC CAGGAGCCTC TGGATTTCAG CCAGCTGACC 
ACAAGGAAAC TGGTGGACAA GGAGGGACTC ATTCCCCCAG AGCTCTTCTA CATGGGGCTA 
ACCGTGTGGG TGAGCAGCGA CCCCCTGGGC CTCGCAGCCT CTCAGGCCAA CTTCTACCCC 
CCACCTCCAG AGTGGCTTCA CGACAAATAT GATACCACCG GGGAGAACCT TCGCAGTGAG 
TCCCATGGGA GCTCGCCCGG AACCTGGTCC TCAGCCCACA CAGGCCCTGA CCCTCCAACC 
CCTCACTGCG CCCACTCCAT CTGGTCCCTC TGCTCCTCCA TCCTCTCCAC AGTCCCGGCA 
GCCCAGCCCT TGGAGTTTGC CCAGTTCCCC TTCCTGCTGC ATGGACTCCA GAAGACTGCA 
GACTTTGTAG AAGCCATCGA AGGGGCCAGG GCGGCATGCA CAGAGGCAGG CCAGGCAGGG 
GTGCATGCCT ACCCCAGTGG CTCCCCCTTC CTCTTCTGGG AGCAGTATCT GGGTCTTCGG 
CGCTGCTTCC TGCTGGCAGT CTGCATCTTG CTGGTGTGCA CCTTCCTCGT CTGTGCCCTG 
CTTCTACTCA GCCCGTGGAC AGCTGGCCTC ATAGTGCTGG TCCTGGCAAT GATGACTGTG 
GAGCTCTTTG GTATCATGGG ATTCCTGGGC ATCAAACTGA GTGCCATCCC CGTGGTAATC 
CTCGTGGCCT CTATAGGCAT TGGTGTTGAA TTCACAGTTC ACGTGGCTCT GGGCTTCCTG 
ACCAGCCATG GTAGCCGGAA CCTGCGGGCT GCTAGCGCTC TGGAACAGAC CTTTGCCCCT 
GTGACTGATG GAGCTGTCTC CACCTTGCTG GGTCTGCTCA TGCTTGCTGG TTCTAACTTT 
GACTTCATCA TAAGGTATTT CTTTGTGGTG CTGACGGTGC TGACACTCTT GGGCCTGCTC 
CATGGACTCC TGCTGCTGCC TGTGCTGCTC TCTATCCTAG GCCCCCCGCC ACAGGTGGTG 
CAGGTATACA AGGAGAGTCC ACAGACCCTA AACTCCGCTG CTCCACAGAG AGGTGGGCTC 
AGGTGGGACA GGCCCCCCAC CCTGCCCCAG AGTTTTGCCA GAGTGACTAC CTCCATGACT 
GTGGCCCTCC ACCCACCACC TCTGCCTGGA GCATACGTCC ACCCAGCCTC CGAGGAGCCC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptch2 ( XM_011240471.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238773.1 Mus musculus patched 2 (Ptch2), transcript variant X2, mRNA.

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CloneID	OMu68511
Clone ID Related Accession (Same CDS sequence)	XM_011240471.2
Accession Version	XM_011240471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGCTGGCC 
TATGCCTGCG TAACAATGCT GCGGTGGGAC TGCGCCCAGT CCCAGGGTGC CGTAGGTCTT 
GCTGGGGTGT TGCTGGTGGC CCTGGCGGTG GCCTCAGGTC TTGGGCTTTG TGCCCTACTT 
GGCATCACTT TCAATGCTGC CACTACACAG GTGCTGCCCT TCTTGGCTCT GGGCATTGGC 
GTGGATGACA TCTTCCTGCT GGCGCATGCC TTCACAAAAG CCCCACCTGA CACCCCTCTC 
CCAGAGCGTA TGGGTGAGTG TTTGAGGAGC ACGGGCACCA GCGTGGCACT CACATCCGTC 
AACAACATGG TCGCCTTTTT CATGGCTGCC CTGGTTCCCA TTCCTGCGCT GCGGGCCTTC 
TCCCTGCAGG CAGCCATAGT GGTCGGCTGC AACTTTGCAG CGGTAATGCT CGTCTTCCCT 
GCCATCCTCA GCCTCGACCT GCGCCGGCGC CACCGCCAAC GCCTGGACGT TCTCTGCTGC 
TTTTCTAGCC CCTGCTCCGC TCAGGTCATT CAGATGCTGC CCCAGGAGCT AGGGGACAGA 
GCAGTACCAG TGGGCATTGC CCACCTGACT GCCACCGTGC AAGCCTTCAC CCACTGCGAA 
GCCAGCAGCC AGCATGTAGT CACCATTTTG CCTCCTCAAG CCCACCTGCT GTCTCCAGCT 
TCTGACCCAC TGGGCTCCGA ACTCTATAGC CCTGGAGGGT CTACACGGGA CCTTCTCAGT 
CAGGAGGAGG GGACAGGGCC ACAGGCGGCC TGCAGGCCCC TGCTCTGTGC CCACTGGACT 
CTCGCCCATT TTGCCCGCTA TCAGTTTGCA CCTTTACTGC TCCAGACACG AGCCAAGGCC 
CTGGTGTTGC TGTTCTTTGG GGCTCTTTTG GGCCTGAGCC TCTATGGAGC CACCTTGGTA 
CAAGATGGGC TGGCCCTGAC AGATGTGGTC CCTAGGGGCA CCAAGGAACA TGCTTTCCTG 
AGCGCCCAGC TCAGGTACTT CTCCCTGTAC GAGGTGGCTC TAGTGACACA GGGTGGCTTT 
GACTACGCCC ACTCCCAACG CGCCCTCTTT GATCTGCACC AGCGCTTCAG CTCCCTCAAG 
GCTGTGCTGC CCCCACCTGC CACCCAGGCA CCCCGCACCT GGCTTCACTA CTACCGCAGC 
TGGCTACAGG GTATCCAGGC TGCATTTGAC CAAGACTGGG CTTCTGGGCG CATCACCTGC 
CACTCTTACC GCAACGGCTC AGAAGATGGC GCCCTGGCCT ACAAGCTGCT CATCCAAACC 
GGGAATGCCC AGGAGCCTCT GGATTTCAGC CAGCTGACCA CAAGGAAACT GGTGGACAAG 
GAGGGACTCA TTCCCCCAGA GCTCTTCTAC ATGGGGCTAA CCGTGTGGGT GAGCAGCGAC 
CCCCTGGGCC TCGCAGCCTC TCAGGCCAAC TTCTACCCCC CACCTCCAGA GTGGCTTCAC 
GACAAATATG ATACCACCGG GGAGAACCTT CGCAGTGAGT CCCATGGGAG CTCGCCCGGA 
ACCTGGTCCT CAGCCCACAC AGGCCCTGAC CCTCCAACCC CTCACTGCGC CCACTCCATC 
TGGTCCCTCT GCTCCTCCAT CCTCTCCACA GTCCCGGCAG CCCAGCCCTT GGAGTTTGCC 
CAGTTCCCCT TCCTGCTGCA TGGACTCCAG AAGACTGCAG ACTTTGTAGA AGCCATCGAA 
GGGGCCAGGG CGGCATGCAC AGAGGCAGGC CAGGCAGGGG TGCATGCCTA CCCCAGTGGC 
TCCCCCTTCC TCTTCTGGGA GCAGTATCTG GGTCTTCGGC GCTGCTTCCT GCTGGCAGTC 
TGCATCTTGC TGGTGTGCAC CTTCCTCGTC TGTGCCCTGC TTCTACTCAG CCCGTGGACA 
GCTGGCCTCA TAGTGCTGGT CCTGGCAATG ATGACTGTGG AGCTCTTTGG TATCATGGGA 
TTCCTGGGCA TCAAACTGAG TGCCATCCCC GTGGTAATCC TCGTGGCCTC TATAGGCATT 
GGTGTTGAAT TCACAGTTCA CGTGGCTCTG GGCTTCCTGA CCAGCCATGG TAGCCGGAAC 
CTGCGGGCTG CTAGCGCTCT GGAACAGACC TTTGCCCCTG TGACTGATGG AGCTGTCTCC 
ACCTTGCTGG GTCTGCTCAT GCTTGCTGGT TCTAACTTTG ACTTCATCAT AAGGTATTTC 
TTTGTGGTGC TGACGGTGCT GACACTCTTG GGCCTGCTCC ATGGACTCCT GCTGCTGCCT 
GTGCTGCTCT CTATCCTAGG CCCCCCGCCA CAGGTGGTGC AGGTATACAA GGAGAGTCCA 
CAGACCCTAA ACTCCGCTGC TCCACAGAGA GGTGGGCTCA GGTGGGACAG GCCCCCCACC 
CTGCCCCAGA GTTTTGCCAG AGTGACTACC TCCATGACTG TGGCCCTCCA CCCACCACCT 
CTGCCTGGAG CATACGTCCA CCCAGCCTCC GAGGAGCCCA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238773.1
CDS	573..4217
Translation	MVRPLSLGELPPSYTPPARSSAPHILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVAFGALALGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTAHQEGGNVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASMVLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPERMGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQRLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDPLGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLTTRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRSESHGSSPGTWSSAHTGPDPPTPHCAHSIWSLCSSILSTVPAAQPLEFAQFPFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLRAASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQVVQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT

Target ORF information:

RefSeq Version	XM_011240471.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patched 2 (Ptch2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240471.2

ORF Insert Sequence:

ATGGTTCGGC CACTGTCCCT TGGAGAGCTA CCTCCCAGCT ACACACCTCC AGCTCGGTCC 
TCCGCACCTC ATATCCTAGC TGGGAGCCTG CAGGCTCCTC TCTGGCTTCG AGCTTACTTC 
CAAGGTCTAC TCTTCTCCCT GGGGTGCAGG ATCCAGAAAC ACTGCGGCAA AGTGCTCTTC 
CTGGGACTGG TGGCCTTTGG GGCTCTGGCA CTGGGTCTCC GAGTGGCTGT AATTGAGACA 
GACCTAGAAC AGCTCTGGGT AGAAGTGGGC AGCAGGGTGA GCCAGGAGCT GCACTACACC 
AAGGAGAAGC TGGGGGAAGA GGCTGCGTAC ACCTCCCAGA TGTTGATTCA GACTGCGCAC 
CAGGAAGGGG GAAACGTCCT CACCCCCGAG GCACTTGACT TGCACCTCCA GGCGGCGCTC 
ACTGCCAGTA AAGTCCAAGT ATCACTCTAT GGGAAATCCT GGGATTTGAA CAAGATCTGC 
TACAAATCAG GGGTTCCCCT TATTGAAAAT GGGATGATCG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGTG TGATCCTCAC CCCGCTTGAC TGCTTCTGGG AAGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCTGAT ATCCAGTGGA CCAACCTGGA CCCTCAGCAG 
CTGCTCGAGG AGCTGGGCCC CTTTGCCTCT CTGGAGGGCT TCCGGGAACT GCTAGATAAG 
GCACAGGTGG GCCAGGCCTA TGTGGGGCGA CCCTGTCTGG ACCCTGATGA CCCCCACTGT 
CCACCTAGTG CTCCCAACCG GCACAGCAGG CAGGCTCCCA ATGTGGCTCA GGAGCTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTC ATGCACTGGC AGGAGGAACT GCTACTAGGG 
GGCACAGCCA GAGATCTCCA AGGACAGCTG CTGAGGGCAG AGGCCTTGCA GAGCACCTTC 
CTGCTCATGA GTCCCCGTCA GCTGTACGAG CACTTCCGGG GCGACTACCA GACACATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCAGCATG GTGCTGCAGG CCTGGCAGAG GCGCTTTGTG 
CAGCTAGCCC AGGAGGCTCT GCCGGCCAAC GCGTCCCAGC AGATCCATGC CTTCTCCTCC 
ACCACCCTGG ATGACATCCT GCGCGCGTTC TCTGAAGTCA GCACCACCCG TGTGCTGGCC 
TATGCCTGCG TAACAATGCT GCGGTGGGAC TGCGCCCAGT CCCAGGGTGC CGTAGGTCTT 
GCTGGGGTGT TGCTGGTGGC CCTGGCGGTG GCCTCAGGTC TTGGGCTTTG TGCCCTACTT 
GGCATCACTT TCAATGCTGC CACTACACAG GTGCTGCCCT TCTTGGCTCT GGGCATTGGC 
GTGGATGACA TCTTCCTGCT GGCGCATGCC TTCACAAAAG CCCCACCTGA CACCCCTCTC 
CCAGAGCGTA TGGGTGAGTG TTTGAGGAGC ACGGGCACCA GCGTGGCACT CACATCCGTC 
AACAACATGG TCGCCTTTTT CATGGCTGCC CTGGTTCCCA TTCCTGCGCT GCGGGCCTTC 
TCCCTGCAGG CAGCCATAGT GGTCGGCTGC AACTTTGCAG CGGTAATGCT CGTCTTCCCT 
GCCATCCTCA GCCTCGACCT GCGCCGGCGC CACCGCCAAC GCCTGGACGT TCTCTGCTGC 
TTTTCTAGCC CCTGCTCCGC TCAGGTCATT CAGATGCTGC CCCAGGAGCT AGGGGACAGA 
GCAGTACCAG TGGGCATTGC CCACCTGACT GCCACCGTGC AAGCCTTCAC CCACTGCGAA 
GCCAGCAGCC AGCATGTAGT CACCATTTTG CCTCCTCAAG CCCACCTGCT GTCTCCAGCT 
TCTGACCCAC TGGGCTCCGA ACTCTATAGC CCTGGAGGGT CTACACGGGA CCTTCTCAGT 
CAGGAGGAGG GGACAGGGCC ACAGGCGGCC TGCAGGCCCC TGCTCTGTGC CCACTGGACT 
CTCGCCCATT TTGCCCGCTA TCAGTTTGCA CCTTTACTGC TCCAGACACG AGCCAAGGCC 
CTGGTGTTGC TGTTCTTTGG GGCTCTTTTG GGCCTGAGCC TCTATGGAGC CACCTTGGTA 
CAAGATGGGC TGGCCCTGAC AGATGTGGTC CCTAGGGGCA CCAAGGAACA TGCTTTCCTG 
AGCGCCCAGC TCAGGTACTT CTCCCTGTAC GAGGTGGCTC TAGTGACACA GGGTGGCTTT 
GACTACGCCC ACTCCCAACG CGCCCTCTTT GATCTGCACC AGCGCTTCAG CTCCCTCAAG 
GCTGTGCTGC CCCCACCTGC CACCCAGGCA CCCCGCACCT GGCTTCACTA CTACCGCAGC 
TGGCTACAGG GTATCCAGGC TGCATTTGAC CAAGACTGGG CTTCTGGGCG CATCACCTGC 
CACTCTTACC GCAACGGCTC AGAAGATGGC GCCCTGGCCT ACAAGCTGCT CATCCAAACC 
GGGAATGCCC AGGAGCCTCT GGATTTCAGC CAGCTGACCA CAAGGAAACT GGTGGACAAG 
GAGGGACTCA TTCCCCCAGA GCTCTTCTAC ATGGGGCTAA CCGTGTGGGT GAGCAGCGAC 
CCCCTGGGCC TCGCAGCCTC TCAGGCCAAC TTCTACCCCC CACCTCCAGA GTGGCTTCAC 
GACAAATATG ATACCACCGG GGAGAACCTT CGCAGTGAGT CCCATGGGAG CTCGCCCGGA 
ACCTGGTCCT CAGCCCACAC AGGCCCTGAC CCTCCAACCC CTCACTGCGC CCACTCCATC 
TGGTCCCTCT GCTCCTCCAT CCTCTCCACA GTCCCGGCAG CCCAGCCCTT GGAGTTTGCC 
CAGTTCCCCT TCCTGCTGCA TGGACTCCAG AAGACTGCAG ACTTTGTAGA AGCCATCGAA 
GGGGCCAGGG CGGCATGCAC AGAGGCAGGC CAGGCAGGGG TGCATGCCTA CCCCAGTGGC 
TCCCCCTTCC TCTTCTGGGA GCAGTATCTG GGTCTTCGGC GCTGCTTCCT GCTGGCAGTC 
TGCATCTTGC TGGTGTGCAC CTTCCTCGTC TGTGCCCTGC TTCTACTCAG CCCGTGGACA 
GCTGGCCTCA TAGTGCTGGT CCTGGCAATG ATGACTGTGG AGCTCTTTGG TATCATGGGA 
TTCCTGGGCA TCAAACTGAG TGCCATCCCC GTGGTAATCC TCGTGGCCTC TATAGGCATT 
GGTGTTGAAT TCACAGTTCA CGTGGCTCTG GGCTTCCTGA CCAGCCATGG TAGCCGGAAC 
CTGCGGGCTG CTAGCGCTCT GGAACAGACC TTTGCCCCTG TGACTGATGG AGCTGTCTCC 
ACCTTGCTGG GTCTGCTCAT GCTTGCTGGT TCTAACTTTG ACTTCATCAT AAGGTATTTC 
TTTGTGGTGC TGACGGTGCT GACACTCTTG GGCCTGCTCC ATGGACTCCT GCTGCTGCCT 
GTGCTGCTCT CTATCCTAGG CCCCCCGCCA CAGGTGGTGC AGGTATACAA GGAGAGTCCA 
CAGACCCTAA ACTCCGCTGC TCCACAGAGA GGTGGGCTCA GGTGGGACAG GCCCCCCACC 
CTGCCCCAGA GTTTTGCCAG AGTGACTACC TCCATGACTG TGGCCCTCCA CCCACCACCT 
CTGCCTGGAG CATACGTCCA CCCAGCCTCC GAGGAGCCCA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activated Hedgehog-GLI Signaling Causes Congenital Ureteropelvic Junction Obstruction.
Journal of the American Society of Nephrology : JASN(2017 Nov)
Sheybani-Deloui S,Chi L,Staite MV,Cain JE,Nieman BJ,Henkelman RM,Wainwright BJ,Potter SS,Bagli DJ,Lorenzo AJ,Rosenblum ND

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Ptch2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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