Psmb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psmb1
Entrez Gene ID	19170
Full Name	proteasome (prosome, macropain) subunit, beta type 1
Synonyms	AA409053,C81484,Lmpc5
General protein information	Preferred Names proteasome (prosome, macropain) subunit, beta type 1 Names proteasome subunit beta type-1 component C5 of proteasome macropain subunit C5 multicatalytic endopeptidase complex subunit C5 proteasome beta-subunit C5 proteasome component C5 proteasome gamma chain proteasome subunit C5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A2\|17 8.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011185.3	NP_035315.1	proteasome subunit beta type-1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	psmb1	NP_001003889.1
Drosophila melanogaster (fruit fly)	Pros26	NP_524115.1
Schizosaccharomyces pombe (fission yeast)	pam1	NP_594729.1
Magnaporthe oryzae (rice blast fungus)	MGG_04622	XP_003710882.1
Neurospora crassa	NCU09366	XP_963776.2
Homo sapiens (human)	PSMB1	NP_002784.1
Mus musculus (house mouse)	Psmb1	NP_035315.1
Caenorhabditis elegans (roundworm)	pbs-6	NP_498806.1
	PSMB1	XP_001084784.1
Bos taurus (cattle)	PSMB1	NP_001033628.1
Gallus gallus (chicken)	PSMB1	NP_001007906.1
Kluyveromyces lactis	KLLA0E05633g	XP_454200.1
Oryza sativa (rice)	Os08g0529100	NP_001062318.1
Canis lupus familiaris (dog)	PSMB1	XP_532275.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004991	XP_315096.4
Xenopus tropicalis (tropical clawed frog)	psmb1	NP_988993.1
Pan troglodytes (chimpanzee)	PSMB1	XP_003311646.1
Rattus norvegicus (Norway rat)	Psmb1	NP_446042.1
Saccharomyces cerevisiae (baker's yeast)	PRE7	NP_009512.1
Eremothecium gossypii	AGOS_AGL324W	NP_986343.2
Arabidopsis thaliana (thale cress)	PBF1	NP_001030905.1
Oryza sativa (rice)	Os09g0505600	NP_001063603.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of proteasome activity by P2Y
de Diego-Garc?a L, Ram?rez-Escudero M, Sebasti?n-Serrano ?, Diaz-Hern?ndez JI, Pintor J, Lucas JJ, D?az-Hern?ndez M
Biochimica et biophysica acta1863(1)43-51(2017 Jan)

Sequence analysis of ?-subunit genes of the 20S proteasome in patients with relapsed multiple myeloma treated with bortezomib or dexamethasone.
Lichter DI, Danaee H, Pickard MD, Tayber O, Sintchak M, Shi H, Richardson PG, Cavenagh J, Blad? J, Fa?on T, Niesvizky R, Alsina M, Dalton W, Sonneveld P, Lonial S, van de Velde H, Ricci D, Esseltine DL, Trepicchio WL, Mulligan G, Anderson KC
Blood120(23)4513-6(2012 Nov)

Characterization of potential S-nitrosylation sites in the myocardium.
Kohr MJ, Aponte AM, Sun J, Wang G, Murphy E, Gucek M, Steenbergen C
American journal of physiology. Heart and circulatory physiology300(4)H1327-35(2011 Apr)

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Sasaki K, Hamazaki J, Koike M, Hirano Y, Komatsu M, Uchiyama Y, Tanaka K, Murata S
Molecular and cellular biology30(15)3864-74(2010 Aug)

A mouse speciation gene encodes a meiotic histone H3 methyltransferase.
Mihola O, Trachtulec Z, Vlcek C, Schimenti JC, Forejt J
Science (New York, N.Y.)323(5912)373-5(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that treatment-emergent resistance to single-agent bortezomib was independent of variants in the proteasome genes PSMB1, PSMB5, PSMB6, PSMB8, PSMB9, and PSMB10.
Title: Sequence analysis of ?-subunit genes of the 20S proteasome in patients with relapsed multiple myeloma treated with bortezomib or dexamethasone.

The following Psmb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psmb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07624	NM_011185.3 Latest version!	Mus musculus proteasome (prosome, macropain) subunit, beta type 1 (Psmb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psmb1 ( NM_011185.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035315.1 Mus musculus proteasome (prosome, macropain) subunit, beta type 1 (Psmb1), mRNA.

Price & Availability↑

CloneID	OMu07624
Clone ID Related Accession (Same CDS sequence)	NM_011185.3
Accession Version	NM_011185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	proteasome subunit beta type-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK132161.1. On Apr 6, 2007 this sequence version replaced NM_011185.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132161.1, SRR1660811.423420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGCTTTCCA CTGCTGCTTA CCGAGACGTG GAACGAGAGC TGGGAATGGG GCCTCACGGC 
TCCGCCGGCC CTGTGCAGCT GCGGTTTTCG CCTTATGCCT TCAACGGAGG TACTGTATTG 
GCAATTGCTG GAGAAGATTT TTCCATCGTC GCTTCAGACA CTCGATTGAG TGAAGGATTT 
TCAATTCATA CCCGAGATAG CCCCAAATGC TACAAACTGA CAGACAAGAC AGTAATTGGC 
TGCAGTGGTT TCCATGGAGA TTGTCTCACT TTGACAAAGA TTATTGAAGC AAGATTAAAG 
ATGTACAAGC ATTCCAATAA CAAGGCCATG ACAACGGGGG CGATTGCTGC AATGCTGTCT 
ACCATCCTGT ACTCACGGCG CTTCTTCCCT TACTATGTTT ACAACATCAT TGGAGGACTT 
GATGAAGAAG GAAAGGGAGC TGTGTACAGC TTTGACCCAG TGGGCTCTTA CCAGAGAGAC 
TCTTTCAAGG CGGGAGGCTC AGCAAGTGCC ATGCTGCAGC CTCTGCTCGA CAACCAGGTT 
GGCTTCAAAA ATATGCAGAA TGTAGAGCAC GTCCCCCTGA CGCTGGACAG AGCCATGAGG 
CTGGTGAAAG ATGTCTTCAT TTCTGCAGCC GAGAGGGATG TGTATACTGG AGATGCTCTC 
AGGATCTGCA TCGTGACCAA AGAGGGCATC AGGGAGGAAA CTGTTCCCCT GCGGAAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035315.1
CDS	15..737
Translation	MLSTAAYRDVERELGMGPHGSAGPVQLRFSPYAFNGGTVLAIAGEDFSIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLTLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVPLRKD

Target ORF information:

RefSeq Version	NM_011185.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) subunit, beta type 1 (Psmb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011185.3

ORF Insert Sequence:

ATGCTTTCCA CTGCTGCTTA CCGAGACGTG GAACGAGAGC TGGGAATGGG GCCTCACGGC 
TCCGCCGGCC CTGTGCAGCT GCGGTTTTCG CCTTATGCCT TCAACGGAGG TACTGTATTG 
GCAATTGCTG GAGAAGATTT TTCCATCGTC GCTTCAGACA CTCGATTGAG TGAAGGATTT 
TCAATTCATA CCCGAGATAG CCCCAAATGC TACAAACTGA CAGACAAGAC AGTAATTGGC 
TGCAGTGGTT TCCATGGAGA TTGTCTCACT TTGACAAAGA TTATTGAAGC AAGATTAAAG 
ATGTACAAGC ATTCCAATAA CAAGGCCATG ACAACGGGGG CGATTGCTGC AATGCTGTCT 
ACCATCCTGT ACTCACGGCG CTTCTTCCCT TACTATGTTT ACAACATCAT TGGAGGACTT 
GATGAAGAAG GAAAGGGAGC TGTGTACAGC TTTGACCCAG TGGGCTCTTA CCAGAGAGAC 
TCTTTCAAGG CGGGAGGCTC AGCAAGTGCC ATGCTGCAGC CTCTGCTCGA CAACCAGGTT 
GGCTTCAAAA ATATGCAGAA TGTAGAGCAC GTCCCCCTGA CGCTGGACAG AGCCATGAGG 
CTGGTGAAAG ATGTCTTCAT TTCTGCAGCC GAGAGGGATG TGTATACTGG AGATGCTCTC 
AGGATCTGCA TCGTGACCAA AGAGGGCATC AGGGAGGAAA CTGTTCCCCT GCGGAAAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of proteasome activity by P2Y
Biochimica et biophysica acta1863(1)43-51(2017 Jan)
de Diego-Garc?a L,Ram?rez-Escudero M,Sebasti?n-Serrano ?,Diaz-Hern?ndez JI,Pintor J,Lucas JJ,D?az-Hern?ndez M

Sequence analysis of ?-subunit genes of the 20S proteasome in patients with relapsed multiple myeloma treated with bortezomib or dexamethasone.
Blood120(23)4513-6(2012 Nov)
Lichter DI,Danaee H,Pickard MD,Tayber O,Sintchak M,Shi H,Richardson PG,Cavenagh J,Blad? J,Fa?on T,Niesvizky R,Alsina M,Dalton W,Sonneveld P,Lonial S,van de Velde H,Ricci D,Esseltine DL,Trepicchio WL,Mulligan G,Anderson KC

Characterization of potential S-nitrosylation sites in the myocardium.
American journal of physiology. Heart and circulatory physiology300(4)H1327-35(2011 Apr)
Kohr MJ,Aponte AM,Sun J,Wang G,Murphy E,Gucek M,Steenbergen C

PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Molecular and cellular biology30(15)3864-74(2010 Aug)
Sasaki K,Hamazaki J,Koike M,Hirano Y,Komatsu M,Uchiyama Y,Tanaka K,Murata S

A mouse speciation gene encodes a meiotic histone H3 methyltransferase.
Science (New York, N.Y.)323(5912)373-5(2009 Jan)
Mihola O,Trachtulec Z,Vlcek C,Schimenti JC,Forejt J