Psap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psap
Entrez Gene ID	19156
Full Name	prosaposin
Synonyms	AI037048,SGP-1
General protein information	Preferred Names prosaposin Names prosaposin snoRNA MBII-198 sulfated glycoprotein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B4\|10 30.02 cM
Summary	This gene encodes a multifunctional glycoprotein that plays a role in the intracellular metabolism of various sphingolipids or secreted into the plasma, milk or cerebrospinal fluid. The encoded protein undergoes proteolytic processing to generate four different polypeptides known as saposin A, B, C or D, that are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. Alternately, the encoded protein is secreted into body fluids where it exhibits neurotrophic and myelinotrophic activities. A complete lack of the encoded protein is fatal to mice either at the neonatal stage or within the first month due to severe leukodystrophy and sphingolipid accumulation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature saposins. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001146124.1	NP_001139596.1	prosaposin isoform F
NM_001146120.1	NP_001139592.1	prosaposin isoform A precursor
NM_011179.3	NP_035309.3	prosaposin isoform B precursor
NM_001146121.1	NP_001139593.1	prosaposin isoform C precursor
NM_001146123.1	NP_001139595.1	prosaposin isoform E precursor
XM_017313848.1	XP_017169337.1	prosaposin isoform X1
XM_017313849.1	XP_017169338.1	prosaposin isoform X2
NM_001146122.1	NP_001139594.1	prosaposin isoform D precursor

Pathways from BioSystems

Homologies

	PSAP	XP_001107102.1
Mus musculus (house mouse)	Psap	NP_001139593.1
Gallus gallus (chicken)	PSAP	NP_990142.1
Drosophila melanogaster (fruit fly)	Sap-r	NP_524597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001082	XP_003437209.1
Xenopus tropicalis (tropical clawed frog)	psap	NP_001017285.1
Canis lupus familiaris (dog)	PSAP	XP_005619049.1
Bos taurus (cattle)	PSAP	NP_776586.1
Rattus norvegicus (Norway rat)	Psap	NP_001177166.1
Danio rerio (zebrafish)	psap	NP_571958.1
Homo sapiens (human)	PSAP	NP_001035931.1
Pan troglodytes (chimpanzee)	PSAP	XP_507840.2

Gene Ontology
Bibliography

Related articles in PubMed

Mechano-Signal Transduction in Mesenchymal Stem Cells Induces Prosaposin Secretion to Drive the Proliferation of Breast Cancer Cells.
Ishihara S, Inman DR, Li WJ, Ponik SM, Keely PJ
Cancer research77(22)6179-6189(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that matrix (ECM) stiffness leads to mechano-signal transduction in mesenchymal stem cells (MSC), which promotes mammary tumor growth in part through secretion of the signaling protein prosaposin.
Title: Mechano-Signal Transduction in Mesenchymal Stem Cells Induces Prosaposin Secretion to Drive the Proliferation of Breast Cancer Cells.

Prosaposin facilitates sortilin-independent lysosomal trafficking of progranulin.
Title: Prosaposin facilitates sortilin-independent lysosomal trafficking of progranulin.

This study demonistrated that Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice.
Title: Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice.

Data suggested that the abundance of Psap in sperm sample may be a sensitive endpoint to predict PCB exposure.
Title: Decrease in prosaposin in spermatozoon is associated with polychlorinated biphenyl exposure.

Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models.
Title: Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation.

The following Psap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18922	NM_011179.3 Latest version!	Mus musculus prosaposin (Psap), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$328.30 $469.00
OMu18962	NM_001146121.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu18994	NM_001146124.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00
OMu19012	NM_001146122.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19024	NM_001146123.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19038	NM_001146120.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$328.30 $469.00
OMu77463	XM_017313848.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu77464	XM_017313849.1 Latest version!	Mus musculus prosaposin (Psap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psap ( NM_011179.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035309.3 Mus musculus prosaposin (Psap), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18922
Clone ID Related Accession (Same CDS sequence)	NM_011179.3
Accession Version	NM_011179.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prosaposin isoform B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY325400.1, AK148545.1 and S36200.1. On Mar 28, 2009 this sequence version replaced NM_011179.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (B). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.223430.1, SRR1660815.110933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGCTGGCT GGCTTCTGTA ATGAGGTCAA GAGAGTGCCA 
ATGAAGACTC TGGTCCCTGC CACCGAGACC ATTAAGAACA TCCTCCCTGC CCTGGAGATG 
ATGGACCCCT ATGAGCAGAA TCTGGTCCAG GCCCACAATG TGATTTTATG CCAGACCTGT 
CAGTTTGTGA TGAATAAGTT TTCTGAGCTG ATTGTCAATA ATGCCACTGA GGAGCTCCTA 
GTTAAAGGTT TGAGCAACGC ATGCGCACTG CTCCCCGATC CTGCCAGAAC CAAGTGCCAG 
GAGGTGGTGG GAACATTTGG CCCCTCCCTG TTGGACATCT TTATCCATGA GGTAAACCCC 
AGCTCTCTGT GCGGTGTGAT CGGCCTCTGT GCTGCCCGCC CGGAGTTGGT GGAGGCACTT 
GAGCAGCCTG CGCCAGCCAT TGTATCTGCA CTGCTCAAAG AGCCCACACC GCCAAAGCAG 
CCCGCACAGC CCAAGCAGTC GGCATTGCCC GCCCATGTGC CTCCTCAGAA GAATGGTGGG 
TTCTGTGAGG TGTGCAAGAA ACTGGTCCTC TATTTGGAAC ATAACCTGGA GAAAAACAGC 
ACCAAGGAGG AAATCCTGGC CGCACTTGAG AAGGGCTGCA GCTTCCTGCC AGACCCTTAC 
CAGAAGCAGT GCGATGACTT TGTGGCTGAG TATGAGCCCT TGCTATTGGA GATCCTCGTG 
GAAGTGATGG ATCCTGGATT TGTGTGCTCG AAAATTGGAG TTTGCCCTTC TGCCTATAAG 
CTGCTGCTGG GAACCGAGAA GTGTGTCTGG GGCCCTAGCT ACTGGTGTCA GAACATGGAG 
ACTGCCGCCC GATGCAATGC TGTCGATCAT TGCAAACGCC ATGTGTGGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035309.3
CDS	116..1789
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_011179.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011179.3

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGCTGGCT GGCTTCTGTA ATGAGGTCAA GAGAGTGCCA 
ATGAAGACTC TGGTCCCTGC CACCGAGACC ATTAAGAACA TCCTCCCTGC CCTGGAGATG 
ATGGACCCCT ATGAGCAGAA TCTGGTCCAG GCCCACAATG TGATTTTATG CCAGACCTGT 
CAGTTTGTGA TGAATAAGTT TTCTGAGCTG ATTGTCAATA ATGCCACTGA GGAGCTCCTA 
GTTAAAGGTT TGAGCAACGC ATGCGCACTG CTCCCCGATC CTGCCAGAAC CAAGTGCCAG 
GAGGTGGTGG GAACATTTGG CCCCTCCCTG TTGGACATCT TTATCCATGA GGTAAACCCC 
AGCTCTCTGT GCGGTGTGAT CGGCCTCTGT GCTGCCCGCC CGGAGTTGGT GGAGGCACTT 
GAGCAGCCTG CGCCAGCCAT TGTATCTGCA CTGCTCAAAG AGCCCACACC GCCAAAGCAG 
CCCGCACAGC CCAAGCAGTC GGCATTGCCC GCCCATGTGC CTCCTCAGAA GAATGGTGGG 
TTCTGTGAGG TGTGCAAGAA ACTGGTCCTC TATTTGGAAC ATAACCTGGA GAAAAACAGC 
ACCAAGGAGG AAATCCTGGC CGCACTTGAG AAGGGCTGCA GCTTCCTGCC AGACCCTTAC 
CAGAAGCAGT GCGATGACTT TGTGGCTGAG TATGAGCCCT TGCTATTGGA GATCCTCGTG 
GAAGTGATGG ATCCTGGATT TGTGTGCTCG AAAATTGGAG TTTGCCCTTC TGCCTATAAG 
CTGCTGCTGG GAACCGAGAA GTGTGTCTGG GGCCCTAGCT ACTGGTGTCA GAACATGGAG 
ACTGCCGCCC GATGCAATGC TGTCGATCAT TGCAAACGCC ATGTGTGGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001146121.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139593.1 Mus musculus prosaposin (Psap), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu18962
Clone ID Related Accession (Same CDS sequence)	NM_001146121.1
Accession Version	NM_001146121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prosaposin isoform C precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY325400.1, BC141089.1, AK150645.1 and S36200.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 2, resulting in a shorter protein (isoform C), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC141089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGGATCAG 
CAACCCAAGG AAATCTGTGT GCTGGCTGGC TTCTGTAATG AGGTCAAGAG AGTGCCAATG 
AAGACTCTGG TCCCTGCCAC CGAGACCATT AAGAACATCC TCCCTGCCCT GGAGATGATG 
GACCCCTATG AGCAGAATCT GGTCCAGGCC CACAATGTGA TTTTATGCCA GACCTGTCAG 
TTTGTGATGA ATAAGTTTTC TGAGCTGATT GTCAATAATG CCACTGAGGA GCTCCTAGTT 
AAAGGTTTGA GCAACGCATG CGCACTGCTC CCCGATCCTG CCAGAACCAA GTGCCAGGAG 
GTGGTGGGAA CATTTGGCCC CTCCCTGTTG GACATCTTTA TCCATGAGGT AAACCCCAGC 
TCTCTGTGCG GTGTGATCGG CCTCTGTGCT GCCCGCCCGG AGTTGGTGGA GGCACTTGAG 
CAGCCTGCGC CAGCCATTGT ATCTGCACTG CTCAAAGAGC CCACACCGCC AAAGCAGCCC 
GCACAGCCCA AGCAGTCGGC ATTGCCCGCC CATGTGCCTC CTCAGAAGAA TGGTGGGTTC 
TGTGAGGTGT GCAAGAAACT GGTCCTCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAAA TCCTGGCCGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGCG ATGACTTTGT GGCTGAGTAT GAGCCCTTGC TATTGGAGAT CCTCGTGGAA 
GTGATGGATC CTGGATTTGT GTGCTCGAAA ATTGGAGTTT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCTAGCTACT GGTGTCAGAA CATGGAGACT 
GCCGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139593.1
CDS	116..1786
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001146121.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146121.1

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGGATCAG 
CAACCCAAGG AAATCTGTGT GCTGGCTGGC TTCTGTAATG AGGTCAAGAG AGTGCCAATG 
AAGACTCTGG TCCCTGCCAC CGAGACCATT AAGAACATCC TCCCTGCCCT GGAGATGATG 
GACCCCTATG AGCAGAATCT GGTCCAGGCC CACAATGTGA TTTTATGCCA GACCTGTCAG 
TTTGTGATGA ATAAGTTTTC TGAGCTGATT GTCAATAATG CCACTGAGGA GCTCCTAGTT 
AAAGGTTTGA GCAACGCATG CGCACTGCTC CCCGATCCTG CCAGAACCAA GTGCCAGGAG 
GTGGTGGGAA CATTTGGCCC CTCCCTGTTG GACATCTTTA TCCATGAGGT AAACCCCAGC 
TCTCTGTGCG GTGTGATCGG CCTCTGTGCT GCCCGCCCGG AGTTGGTGGA GGCACTTGAG 
CAGCCTGCGC CAGCCATTGT ATCTGCACTG CTCAAAGAGC CCACACCGCC AAAGCAGCCC 
GCACAGCCCA AGCAGTCGGC ATTGCCCGCC CATGTGCCTC CTCAGAAGAA TGGTGGGTTC 
TGTGAGGTGT GCAAGAAACT GGTCCTCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAAA TCCTGGCCGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGCG ATGACTTTGT GGCTGAGTAT GAGCCCTTGC TATTGGAGAT CCTCGTGGAA 
GTGATGGATC CTGGATTTGT GTGCTCGAAA ATTGGAGTTT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCTAGCTACT GGTGTCAGAA CATGGAGACT 
GCCGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001146124.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139596.1 Mus musculus prosaposin (Psap), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu18994
Clone ID Related Accession (Same CDS sequence)	NM_001146124.1
Accession Version	NM_001146124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prosaposin isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK149785.1, AK159622.1 and S36200.1. Transcript Variant: This variant (6) uses an alternate exon for its 5' UTR and start codon and lacks an exon in the 3' coding region, compared to variant 2, resulting in a protein (isoform F) with a novel N-terminus and shorter C-terminus, compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149785.1, SRR1660815.184392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGGCT CCGGGCAAAC TCTCGTGATG CCTCTGACCA GCCCTGTCCA AGACCCGAAG 
ACATGCTCTG GGGGCTCAGC AGTGCTGTGC AGAGATGTGA AGACGGCGGT GGACTGTGGG 
GCCGTGAAGC ACTGCCAGCA GATGGTCTGG AGCAAGCCCA CAGCGAAATC CCTTCCTTGC 
GACATATGCA AAACTGTTGT CACCGAAGCT GGGAACTTGC TGAAAGATAA TGCTACGCAG 
GAGGAGATCC TTCATTACCT GGAGAAGACC TGTGAGTGGA TTCATGACTC CAGCCTGTCG 
GCCTCGTGCA AGGAGGTGGT TGACTCTTAC CTGCCTGTCA TCCTGGACAT GATTAAGGGC 
GAGATGAGCA ACCCTGGGGA AGTGTGCTCT GCGCTCAACC TCTGCCAGTC CCTTCAGGAG 
TACTTGGCCG AGCAAAACCA GAAACAGCTT GAGTCCAACA AGATCCCGGA GGTGGACATG 
GCCCGTGTGG TTGCCCCCTT CATGTCCAAC ATCCCTCTCC TGCTGTACCC TCAGGATCAC 
CCCCGCAGCC AGCCCCAACC TAAGGCTAAC GAGGACGTCT GCCAGGACTG TATGAAGCTG 
GTGTCTGATG TCCAGACTGC TGTGAAGACC AACTCCAGCT TTATCCAGGG CTTCGTGGAC 
CACGTGAAGG AGGATTGTGA CCGCTTGGGG CCAGGCGTGT CTGACATATG CAAGAACTAC 
GTGGACCAGT ATTCCGAGGT CTGTGTCCAG ATGTTGATGC ACATGCAACC CAAGGAAATC 
TGTGTGCTGG CTGGCTTCTG TAATGAGGTC AAGAGAGTGC CAATGAAGAC TCTGGTCCCT 
GCCACCGAGA CCATTAAGAA CATCCTCCCT GCCCTGGAGA TGATGGACCC CTATGAGCAG 
AATCTGGTCC AGGCCCACAA TGTGATTTTA TGCCAGACCT GTCAGTTTGT GATGAATAAG 
TTTTCTGAGC TGATTGTCAA TAATGCCACT GAGGAGCTCC TAGTTAAAGG TTTGAGCAAC 
GCATGCGCAC TGCTCCCCGA TCCTGCCAGA ACCAAGTGCC AGGAGGTGGT GGGAACATTT 
GGCCCCTCCC TGTTGGACAT CTTTATCCAT GAGGTAAACC CCAGCTCTCT GTGCGGTGTG 
ATCGGCCTCT GTGCTGCCCG CCCGGAGTTG GTGGAGGCAC TTGAGCAGCC TGCGCCAGCC 
ATTGTATCTG CACTGCTCAA AGAGCCCACA CCGCCAAAGC AGCCCGCACA GCCCAAGCAG 
TCGGCATTGC CCGCCCATGT GCCTCCTCAG AAGAATGGTG GGTTCTGTGA GGTGTGCAAG 
AAACTGGTCC TCTATTTGGA ACATAACCTG GAGAAAAACA GCACCAAGGA GGAAATCCTG 
GCCGCACTTG AGAAGGGCTG CAGCTTCCTG CCAGACCCTT ACCAGAAGCA GTGCGATGAC 
TTTGTGGCTG AGTATGAGCC CTTGCTATTG GAGATCCTCG TGGAAGTGAT GGATCCTGGA 
TTTGTGTGCT CGAAAATTGG AGTTTGCCCT TCTGCCTATA AGCTGCTGCT GGGAACCGAG 
AAGTGTGTCT GGGGCCCTAG CTACTGGTGT CAGAACATGG AGACTGCCGC CCGATGCAAT 
GCTGTCGATC ATTGCAAACG CCATGTGTGG AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139596.1
CDS	41..1696
Translation	MGGSGQTLVMPLTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001146124.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146124.1

ORF Insert Sequence:

ATGGGGGGCT CCGGGCAAAC TCTCGTGATG CCTCTGACCA GCCCTGTCCA AGACCCGAAG 
ACATGCTCTG GGGGCTCAGC AGTGCTGTGC AGAGATGTGA AGACGGCGGT GGACTGTGGG 
GCCGTGAAGC ACTGCCAGCA GATGGTCTGG AGCAAGCCCA CAGCGAAATC CCTTCCTTGC 
GACATATGCA AAACTGTTGT CACCGAAGCT GGGAACTTGC TGAAAGATAA TGCTACGCAG 
GAGGAGATCC TTCATTACCT GGAGAAGACC TGTGAGTGGA TTCATGACTC CAGCCTGTCG 
GCCTCGTGCA AGGAGGTGGT TGACTCTTAC CTGCCTGTCA TCCTGGACAT GATTAAGGGC 
GAGATGAGCA ACCCTGGGGA AGTGTGCTCT GCGCTCAACC TCTGCCAGTC CCTTCAGGAG 
TACTTGGCCG AGCAAAACCA GAAACAGCTT GAGTCCAACA AGATCCCGGA GGTGGACATG 
GCCCGTGTGG TTGCCCCCTT CATGTCCAAC ATCCCTCTCC TGCTGTACCC TCAGGATCAC 
CCCCGCAGCC AGCCCCAACC TAAGGCTAAC GAGGACGTCT GCCAGGACTG TATGAAGCTG 
GTGTCTGATG TCCAGACTGC TGTGAAGACC AACTCCAGCT TTATCCAGGG CTTCGTGGAC 
CACGTGAAGG AGGATTGTGA CCGCTTGGGG CCAGGCGTGT CTGACATATG CAAGAACTAC 
GTGGACCAGT ATTCCGAGGT CTGTGTCCAG ATGTTGATGC ACATGCAACC CAAGGAAATC 
TGTGTGCTGG CTGGCTTCTG TAATGAGGTC AAGAGAGTGC CAATGAAGAC TCTGGTCCCT 
GCCACCGAGA CCATTAAGAA CATCCTCCCT GCCCTGGAGA TGATGGACCC CTATGAGCAG 
AATCTGGTCC AGGCCCACAA TGTGATTTTA TGCCAGACCT GTCAGTTTGT GATGAATAAG 
TTTTCTGAGC TGATTGTCAA TAATGCCACT GAGGAGCTCC TAGTTAAAGG TTTGAGCAAC 
GCATGCGCAC TGCTCCCCGA TCCTGCCAGA ACCAAGTGCC AGGAGGTGGT GGGAACATTT 
GGCCCCTCCC TGTTGGACAT CTTTATCCAT GAGGTAAACC CCAGCTCTCT GTGCGGTGTG 
ATCGGCCTCT GTGCTGCCCG CCCGGAGTTG GTGGAGGCAC TTGAGCAGCC TGCGCCAGCC 
ATTGTATCTG CACTGCTCAA AGAGCCCACA CCGCCAAAGC AGCCCGCACA GCCCAAGCAG 
TCGGCATTGC CCGCCCATGT GCCTCCTCAG AAGAATGGTG GGTTCTGTGA GGTGTGCAAG 
AAACTGGTCC TCTATTTGGA ACATAACCTG GAGAAAAACA GCACCAAGGA GGAAATCCTG 
GCCGCACTTG AGAAGGGCTG CAGCTTCCTG CCAGACCCTT ACCAGAAGCA GTGCGATGAC 
TTTGTGGCTG AGTATGAGCC CTTGCTATTG GAGATCCTCG TGGAAGTGAT GGATCCTGGA 
TTTGTGTGCT CGAAAATTGG AGTTTGCCCT TCTGCCTATA AGCTGCTGCT GGGAACCGAG 
AAGTGTGTCT GGGGCCCTAG CTACTGGTGT CAGAACATGG AGACTGCCGC CCGATGCAAT 
GCTGTCGATC ATTGCAAACG CCATGTGTGG AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001146122.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139594.1 Mus musculus prosaposin (Psap), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu19012
Clone ID Related Accession (Same CDS sequence)	NM_001146122.1
Accession Version	NM_001146122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prosaposin isoform D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY325400.1, AK159221.1 and S36200.1. Transcript Variant: This variant (4) lacks an exon in the coding region and uses an alternate in-frame splice site, compared to variant 2, resulting in a shorter protein (isoform D), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159221.1, AK159244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAACCC 
AAGGAAATCT GTGTGCTGGC TGGCTTCTGT AATGAGGTCA AGAGAGTGCC AATGAAGACT 
CTGGTCCCTG CCACCGAGAC CATTAAGAAC ATCCTCCCTG CCCTGGAGAT GATGGACCCC 
TATGAGAATC TGGTCCAGGC CCACAATGTG ATTTTATGCC AGACCTGTCA GTTTGTGATG 
AATAAGTTTT CTGAGCTGAT TGTCAATAAT GCCACTGAGG AGCTCCTAGT TAAAGGTTTG 
AGCAACGCAT GCGCACTGCT CCCCGATCCT GCCAGAACCA AGTGCCAGGA GGTGGTGGGA 
ACATTTGGCC CCTCCCTGTT GGACATCTTT ATCCATGAGG TAAACCCCAG CTCTCTGTGC 
GGTGTGATCG GCCTCTGTGC TGCCCGCCCG GAGTTGGTGG AGGCACTTGA GCAGCCTGCG 
CCAGCCATTG TATCTGCACT GCTCAAAGAG CCCACACCGC CAAAGCAGCC CGCACAGCCC 
AAGCAGTCGG CATTGCCCGC CCATGTGCCT CCTCAGAAGA ATGGTGGGTT CTGTGAGGTG 
TGCAAGAAAC TGGTCCTCTA TTTGGAACAT AACCTGGAGA AAAACAGCAC CAAGGAGGAA 
ATCCTGGCCG CACTTGAGAA GGGCTGCAGC TTCCTGCCAG ACCCTTACCA GAAGCAGTGC 
GATGACTTTG TGGCTGAGTA TGAGCCCTTG CTATTGGAGA TCCTCGTGGA AGTGATGGAT 
CCTGGATTTG TGTGCTCGAA AATTGGAGTT TGCCCTTCTG CCTATAAGCT GCTGCTGGGA 
ACCGAGAAGT GTGTCTGGGG CCCTAGCTAC TGGTGTCAGA ACATGGAGAC TGCCGCCCGA 
TGCAATGCTG TCGATCATTG CAAACGCCAT GTGTGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139594.1
CDS	116..1777
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYENLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001146122.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146122.1

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAACCC 
AAGGAAATCT GTGTGCTGGC TGGCTTCTGT AATGAGGTCA AGAGAGTGCC AATGAAGACT 
CTGGTCCCTG CCACCGAGAC CATTAAGAAC ATCCTCCCTG CCCTGGAGAT GATGGACCCC 
TATGAGAATC TGGTCCAGGC CCACAATGTG ATTTTATGCC AGACCTGTCA GTTTGTGATG 
AATAAGTTTT CTGAGCTGAT TGTCAATAAT GCCACTGAGG AGCTCCTAGT TAAAGGTTTG 
AGCAACGCAT GCGCACTGCT CCCCGATCCT GCCAGAACCA AGTGCCAGGA GGTGGTGGGA 
ACATTTGGCC CCTCCCTGTT GGACATCTTT ATCCATGAGG TAAACCCCAG CTCTCTGTGC 
GGTGTGATCG GCCTCTGTGC TGCCCGCCCG GAGTTGGTGG AGGCACTTGA GCAGCCTGCG 
CCAGCCATTG TATCTGCACT GCTCAAAGAG CCCACACCGC CAAAGCAGCC CGCACAGCCC 
AAGCAGTCGG CATTGCCCGC CCATGTGCCT CCTCAGAAGA ATGGTGGGTT CTGTGAGGTG 
TGCAAGAAAC TGGTCCTCTA TTTGGAACAT AACCTGGAGA AAAACAGCAC CAAGGAGGAA 
ATCCTGGCCG CACTTGAGAA GGGCTGCAGC TTCCTGCCAG ACCCTTACCA GAAGCAGTGC 
GATGACTTTG TGGCTGAGTA TGAGCCCTTG CTATTGGAGA TCCTCGTGGA AGTGATGGAT 
CCTGGATTTG TGTGCTCGAA AATTGGAGTT TGCCCTTCTG CCTATAAGCT GCTGCTGGGA 
ACCGAGAAGT GTGTCTGGGG CCCTAGCTAC TGGTGTCAGA ACATGGAGAC TGCCGCCCGA 
TGCAATGCTG TCGATCATTG CAAACGCCAT GTGTGGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001146123.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139595.1 Mus musculus prosaposin (Psap), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu19024
Clone ID Related Accession (Same CDS sequence)	NM_001146123.1
Accession Version	NM_001146123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prosaposin isoform E precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY325400.1, AK151234.1 and S36200.1. Transcript Variant: This variant (5) lacks an exon in the coding region and uses an alternate in-frame splice site, compared to variant 2, resulting in a shorter protein (isoform E), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151234.1, AK151529.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGCAA CCCAAGGAAA TCTGTGTGCT GGCTGGCTTC 
TGTAATGAGG TCAAGAGAGT GCCAATGAAG ACTCTGGTCC CTGCCACCGA GACCATTAAG 
AACATCCTCC CTGCCCTGGA GATGATGGAC CCCTATGAGC AGAATCTGGT CCAGGCCCAC 
AATGTGATTT TATGCCAGAC CTGTCAGTTT GTGATGAATA AGTTTTCTGA GCTGATTGTC 
AATAATGCCA CTGAGGAGCT CCTAGTTAAA GGTTTGAGCA ACGCATGCGC ACTGCTCCCC 
GATCCTGCCA GAACCAAGTG CCAGGAGGTG GTGGGAACAT TTGGCCCCTC CCTGTTGGAC 
ATCTTTATCC ATGAGGTAAA CCCCAGCTCT CTGTGCGGTG TGATCGGCCT CTGTGCTGCC 
CGCCCGGAGT TGGTGGAGGC ACTTGAGCAG CCTGCGCCAG CCATTGTATC TGCACTGCTC 
AAAGAGCCCA CACCGCCAAA GCAGCCCGCA CAGCCCAAGC AGTCGGCATT GCCCGCCCAT 
GTGCCTCCTC AGAAGAATGG TGGGTTCTGT GAGGTGTGCA AGAAACTGGT CCTCTATTTG 
GAACATAACC TGGAGAAAAA CAGCACCAAG GAGGAAATCC TGGCCGCACT TGAGAAGGGC 
TGCAGCTTCC TGCCAGACCC TTACCAGAAG CAGTGCGATG ACTTTGTGGC TGAGTATGAG 
CCCTTGCTAT TGGAGATCCT CGTGGAAGTG ATGGATCCTG GATTTGTGTG CTCGAAAATT 
GGAGTTTGCC CTTCTGCCTA TAAGCTGCTG CTGGGAACCG AGAAGTGTGT CTGGGGCCCT 
AGCTACTGGT GTCAGAACAT GGAGACTGCC GCCCGATGCA ATGCTGTCGA TCATTGCAAA 
CGCCATGTGT GGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139595.1
CDS	116..1753
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001146123.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146123.1

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGCAA CCCAAGGAAA TCTGTGTGCT GGCTGGCTTC 
TGTAATGAGG TCAAGAGAGT GCCAATGAAG ACTCTGGTCC CTGCCACCGA GACCATTAAG 
AACATCCTCC CTGCCCTGGA GATGATGGAC CCCTATGAGC AGAATCTGGT CCAGGCCCAC 
AATGTGATTT TATGCCAGAC CTGTCAGTTT GTGATGAATA AGTTTTCTGA GCTGATTGTC 
AATAATGCCA CTGAGGAGCT CCTAGTTAAA GGTTTGAGCA ACGCATGCGC ACTGCTCCCC 
GATCCTGCCA GAACCAAGTG CCAGGAGGTG GTGGGAACAT TTGGCCCCTC CCTGTTGGAC 
ATCTTTATCC ATGAGGTAAA CCCCAGCTCT CTGTGCGGTG TGATCGGCCT CTGTGCTGCC 
CGCCCGGAGT TGGTGGAGGC ACTTGAGCAG CCTGCGCCAG CCATTGTATC TGCACTGCTC 
AAAGAGCCCA CACCGCCAAA GCAGCCCGCA CAGCCCAAGC AGTCGGCATT GCCCGCCCAT 
GTGCCTCCTC AGAAGAATGG TGGGTTCTGT GAGGTGTGCA AGAAACTGGT CCTCTATTTG 
GAACATAACC TGGAGAAAAA CAGCACCAAG GAGGAAATCC TGGCCGCACT TGAGAAGGGC 
TGCAGCTTCC TGCCAGACCC TTACCAGAAG CAGTGCGATG ACTTTGTGGC TGAGTATGAG 
CCCTTGCTAT TGGAGATCCT CGTGGAAGTG ATGGATCCTG GATTTGTGTG CTCGAAAATT 
GGAGTTTGCC CTTCTGCCTA TAAGCTGCTG CTGGGAACCG AGAAGTGTGT CTGGGGCCCT 
AGCTACTGGT GTCAGAACAT GGAGACTGCC GCCCGATGCA ATGCTGTCGA TCATTGCAAA 
CGCCATGTGT GGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( NM_001146120.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139592.1 Mus musculus prosaposin (Psap), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19038
Clone ID Related Accession (Same CDS sequence)	NM_001146120.1
Accession Version	NM_001146120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prosaposin isoform A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY325400.1, AK151686.1, AK150645.1 and S36200.1. Transcript Variant: This variant (1) lacks an exon in the coding region, compared to variant 2, resulting in a shorter protein (isoform A), compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK167902.1, SRR1660817.131583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAACCC 
AAGGAAATCT GTGTGCTGGC TGGCTTCTGT AATGAGGTCA AGAGAGTGCC AATGAAGACT 
CTGGTCCCTG CCACCGAGAC CATTAAGAAC ATCCTCCCTG CCCTGGAGAT GATGGACCCC 
TATGAGCAGA ATCTGGTCCA GGCCCACAAT GTGATTTTAT GCCAGACCTG TCAGTTTGTG 
ATGAATAAGT TTTCTGAGCT GATTGTCAAT AATGCCACTG AGGAGCTCCT AGTTAAAGGT 
TTGAGCAACG CATGCGCACT GCTCCCCGAT CCTGCCAGAA CCAAGTGCCA GGAGGTGGTG 
GGAACATTTG GCCCCTCCCT GTTGGACATC TTTATCCATG AGGTAAACCC CAGCTCTCTG 
TGCGGTGTGA TCGGCCTCTG TGCTGCCCGC CCGGAGTTGG TGGAGGCACT TGAGCAGCCT 
GCGCCAGCCA TTGTATCTGC ACTGCTCAAA GAGCCCACAC CGCCAAAGCA GCCCGCACAG 
CCCAAGCAGT CGGCATTGCC CGCCCATGTG CCTCCTCAGA AGAATGGTGG GTTCTGTGAG 
GTGTGCAAGA AACTGGTCCT CTATTTGGAA CATAACCTGG AGAAAAACAG CACCAAGGAG 
GAAATCCTGG CCGCACTTGA GAAGGGCTGC AGCTTCCTGC CAGACCCTTA CCAGAAGCAG 
TGCGATGACT TTGTGGCTGA GTATGAGCCC TTGCTATTGG AGATCCTCGT GGAAGTGATG 
GATCCTGGAT TTGTGTGCTC GAAAATTGGA GTTTGCCCTT CTGCCTATAA GCTGCTGCTG 
GGAACCGAGA AGTGTGTCTG GGGCCCTAGC TACTGGTGTC AGAACATGGA GACTGCCGCC 
CGATGCAATG CTGTCGATCA TTGCAAACGC CATGTGTGGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139592.1
CDS	116..1780
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	NM_001146120.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146120.1

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAACCC 
AAGGAAATCT GTGTGCTGGC TGGCTTCTGT AATGAGGTCA AGAGAGTGCC AATGAAGACT 
CTGGTCCCTG CCACCGAGAC CATTAAGAAC ATCCTCCCTG CCCTGGAGAT GATGGACCCC 
TATGAGCAGA ATCTGGTCCA GGCCCACAAT GTGATTTTAT GCCAGACCTG TCAGTTTGTG 
ATGAATAAGT TTTCTGAGCT GATTGTCAAT AATGCCACTG AGGAGCTCCT AGTTAAAGGT 
TTGAGCAACG CATGCGCACT GCTCCCCGAT CCTGCCAGAA CCAAGTGCCA GGAGGTGGTG 
GGAACATTTG GCCCCTCCCT GTTGGACATC TTTATCCATG AGGTAAACCC CAGCTCTCTG 
TGCGGTGTGA TCGGCCTCTG TGCTGCCCGC CCGGAGTTGG TGGAGGCACT TGAGCAGCCT 
GCGCCAGCCA TTGTATCTGC ACTGCTCAAA GAGCCCACAC CGCCAAAGCA GCCCGCACAG 
CCCAAGCAGT CGGCATTGCC CGCCCATGTG CCTCCTCAGA AGAATGGTGG GTTCTGTGAG 
GTGTGCAAGA AACTGGTCCT CTATTTGGAA CATAACCTGG AGAAAAACAG CACCAAGGAG 
GAAATCCTGG CCGCACTTGA GAAGGGCTGC AGCTTCCTGC CAGACCCTTA CCAGAAGCAG 
TGCGATGACT TTGTGGCTGA GTATGAGCCC TTGCTATTGG AGATCCTCGT GGAAGTGATG 
GATCCTGGAT TTGTGTGCTC GAAAATTGGA GTTTGCCCTT CTGCCTATAA GCTGCTGCTG 
GGAACCGAGA AGTGTGTCTG GGGCCCTAGC TACTGGTGTC AGAACATGGA GACTGCCGCC 
CGATGCAATG CTGTCGATCA TTGCAAACGC CATGTGTGGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( XM_017313848.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169337.1 Mus musculus prosaposin (Psap), transcript variant X1, mRNA.

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CloneID	OMu77463
Clone ID Related Accession (Same CDS sequence)	XM_017313848.1
Accession Version	XM_017313848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prosaposin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGCTGGCT GGCTTCTGTA ATGAGGTCAA GAGAGTGCCA 
ATGAAGACTC TGGTCCCTGC CACCGAGACC ATTAAGAACA TCCTCCCTGC CCTGGAGATG 
ATGGACCCCT ATGAGAATCT GGTCCAGGCC CACAATGTGA TTTTATGCCA GACCTGTCAG 
TTTGTGATGA ATAAGTTTTC TGAGCTGATT GTCAATAATG CCACTGAGGA GCTCCTAGTT 
AAAGGTTTGA GCAACGCATG CGCACTGCTC CCCGATCCTG CCAGAACCAA GTGCCAGGAG 
GTGGTGGGAA CATTTGGCCC CTCCCTGTTG GACATCTTTA TCCATGAGGT AAACCCCAGC 
TCTCTGTGCG GTGTGATCGG CCTCTGTGCT GCCCGCCCGG AGTTGGTGGA GGCACTTGAG 
CAGCCTGCGC CAGCCATTGT ATCTGCACTG CTCAAAGAGC CCACACCGCC AAAGCAGCCC 
GCACAGCCCA AGCAGTCGGC ATTGCCCGCC CATGTGCCTC CTCAGAAGAA TGGTGGGTTC 
TGTGAGGTGT GCAAGAAACT GGTCCTCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAAA TCCTGGCCGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGCG ATGACTTTGT GGCTGAGTAT GAGCCCTTGC TATTGGAGAT CCTCGTGGAA 
GTGATGGATC CTGGATTTGT GTGCTCGAAA ATTGGAGTTT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCTAGCTACT GGTGTCAGAA CATGGAGACT 
GCCGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169337.1
CDS	112..1782
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYENLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	XM_017313848.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313848.1

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGCAGGAT 
CAGCAACCCA AGGAAATCTG TGTGCTGGCT GGCTTCTGTA ATGAGGTCAA GAGAGTGCCA 
ATGAAGACTC TGGTCCCTGC CACCGAGACC ATTAAGAACA TCCTCCCTGC CCTGGAGATG 
ATGGACCCCT ATGAGAATCT GGTCCAGGCC CACAATGTGA TTTTATGCCA GACCTGTCAG 
TTTGTGATGA ATAAGTTTTC TGAGCTGATT GTCAATAATG CCACTGAGGA GCTCCTAGTT 
AAAGGTTTGA GCAACGCATG CGCACTGCTC CCCGATCCTG CCAGAACCAA GTGCCAGGAG 
GTGGTGGGAA CATTTGGCCC CTCCCTGTTG GACATCTTTA TCCATGAGGT AAACCCCAGC 
TCTCTGTGCG GTGTGATCGG CCTCTGTGCT GCCCGCCCGG AGTTGGTGGA GGCACTTGAG 
CAGCCTGCGC CAGCCATTGT ATCTGCACTG CTCAAAGAGC CCACACCGCC AAAGCAGCCC 
GCACAGCCCA AGCAGTCGGC ATTGCCCGCC CATGTGCCTC CTCAGAAGAA TGGTGGGTTC 
TGTGAGGTGT GCAAGAAACT GGTCCTCTAT TTGGAACATA ACCTGGAGAA AAACAGCACC 
AAGGAGGAAA TCCTGGCCGC ACTTGAGAAG GGCTGCAGCT TCCTGCCAGA CCCTTACCAG 
AAGCAGTGCG ATGACTTTGT GGCTGAGTAT GAGCCCTTGC TATTGGAGAT CCTCGTGGAA 
GTGATGGATC CTGGATTTGT GTGCTCGAAA ATTGGAGTTT GCCCTTCTGC CTATAAGCTG 
CTGCTGGGAA CCGAGAAGTG TGTCTGGGGC CCTAGCTACT GGTGTCAGAA CATGGAGACT 
GCCGCCCGAT GCAATGCTGT CGATCATTGC AAACGCCATG TGTGGAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psap ( XM_017313849.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169338.1 Mus musculus prosaposin (Psap), transcript variant X2, mRNA.

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CloneID	OMu77464
Clone ID Related Accession (Same CDS sequence)	XM_017313849.1
Accession Version	XM_017313849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prosaposin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGGATCAG 
CAACCCAAGG AAATCTGTGT GCTGGCTGGC TTCTGTAATG AGGTCAAGAG AGTGCCAATG 
AAGACTCTGG TCCCTGCCAC CGAGACCATT AAGAACATCC TCCCTGCCCT GGAGATGATG 
GACCCCTATG AGAATCTGGT CCAGGCCCAC AATGTGATTT TATGCCAGAC CTGTCAGTTT 
GTGATGAATA AGTTTTCTGA GCTGATTGTC AATAATGCCA CTGAGGAGCT CCTAGTTAAA 
GGTTTGAGCA ACGCATGCGC ACTGCTCCCC GATCCTGCCA GAACCAAGTG CCAGGAGGTG 
GTGGGAACAT TTGGCCCCTC CCTGTTGGAC ATCTTTATCC ATGAGGTAAA CCCCAGCTCT 
CTGTGCGGTG TGATCGGCCT CTGTGCTGCC CGCCCGGAGT TGGTGGAGGC ACTTGAGCAG 
CCTGCGCCAG CCATTGTATC TGCACTGCTC AAAGAGCCCA CACCGCCAAA GCAGCCCGCA 
CAGCCCAAGC AGTCGGCATT GCCCGCCCAT GTGCCTCCTC AGAAGAATGG TGGGTTCTGT 
GAGGTGTGCA AGAAACTGGT CCTCTATTTG GAACATAACC TGGAGAAAAA CAGCACCAAG 
GAGGAAATCC TGGCCGCACT TGAGAAGGGC TGCAGCTTCC TGCCAGACCC TTACCAGAAG 
CAGTGCGATG ACTTTGTGGC TGAGTATGAG CCCTTGCTAT TGGAGATCCT CGTGGAAGTG 
ATGGATCCTG GATTTGTGTG CTCGAAAATT GGAGTTTGCC CTTCTGCCTA TAAGCTGCTG 
CTGGGAACCG AGAAGTGTGT CTGGGGCCCT AGCTACTGGT GTCAGAACAT GGAGACTGCC 
GCCCGATGCA ATGCTGTCGA TCATTGCAAA CGCCATGTGT GGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169338.1
CDS	112..1779
Translation	MYALALFASLLATALTSPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYENLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

Target ORF information:

RefSeq Version	XM_017313849.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prosaposin (Psap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313849.1

ORF Insert Sequence:

ATGTACGCCC TCGCCCTCTT CGCCAGCCTT CTGGCCACCG CTCTGACCAG CCCTGTCCAA 
GACCCGAAGA CATGCTCTGG GGGCTCAGCA GTGCTGTGCA GAGATGTGAA GACGGCGGTG 
GACTGTGGGG CCGTGAAGCA CTGCCAGCAG ATGGTCTGGA GCAAGCCCAC AGCGAAATCC 
CTTCCTTGCG ACATATGCAA AACTGTTGTC ACCGAAGCTG GGAACTTGCT GAAAGATAAT 
GCTACGCAGG AGGAGATCCT TCATTACCTG GAGAAGACCT GTGAGTGGAT TCATGACTCC 
AGCCTGTCGG CCTCGTGCAA GGAGGTGGTT GACTCTTACC TGCCTGTCAT CCTGGACATG 
ATTAAGGGCG AGATGAGCAA CCCTGGGGAA GTGTGCTCTG CGCTCAACCT CTGCCAGTCC 
CTTCAGGAGT ACTTGGCCGA GCAAAACCAG AAACAGCTTG AGTCCAACAA GATCCCGGAG 
GTGGACATGG CCCGTGTGGT TGCCCCCTTC ATGTCCAACA TCCCTCTCCT GCTGTACCCT 
CAGGATCACC CCCGCAGCCA GCCCCAACCT AAGGCTAACG AGGACGTCTG CCAGGACTGT 
ATGAAGCTGG TGTCTGATGT CCAGACTGCT GTGAAGACCA ACTCCAGCTT TATCCAGGGC 
TTCGTGGACC ACGTGAAGGA GGATTGTGAC CGCTTGGGGC CAGGCGTGTC TGACATATGC 
AAGAACTACG TGGACCAGTA TTCCGAGGTC TGTGTCCAGA TGTTGATGCA CATGGATCAG 
CAACCCAAGG AAATCTGTGT GCTGGCTGGC TTCTGTAATG AGGTCAAGAG AGTGCCAATG 
AAGACTCTGG TCCCTGCCAC CGAGACCATT AAGAACATCC TCCCTGCCCT GGAGATGATG 
GACCCCTATG AGAATCTGGT CCAGGCCCAC AATGTGATTT TATGCCAGAC CTGTCAGTTT 
GTGATGAATA AGTTTTCTGA GCTGATTGTC AATAATGCCA CTGAGGAGCT CCTAGTTAAA 
GGTTTGAGCA ACGCATGCGC ACTGCTCCCC GATCCTGCCA GAACCAAGTG CCAGGAGGTG 
GTGGGAACAT TTGGCCCCTC CCTGTTGGAC ATCTTTATCC ATGAGGTAAA CCCCAGCTCT 
CTGTGCGGTG TGATCGGCCT CTGTGCTGCC CGCCCGGAGT TGGTGGAGGC ACTTGAGCAG 
CCTGCGCCAG CCATTGTATC TGCACTGCTC AAAGAGCCCA CACCGCCAAA GCAGCCCGCA 
CAGCCCAAGC AGTCGGCATT GCCCGCCCAT GTGCCTCCTC AGAAGAATGG TGGGTTCTGT 
GAGGTGTGCA AGAAACTGGT CCTCTATTTG GAACATAACC TGGAGAAAAA CAGCACCAAG 
GAGGAAATCC TGGCCGCACT TGAGAAGGGC TGCAGCTTCC TGCCAGACCC TTACCAGAAG 
CAGTGCGATG ACTTTGTGGC TGAGTATGAG CCCTTGCTAT TGGAGATCCT CGTGGAAGTG 
ATGGATCCTG GATTTGTGTG CTCGAAAATT GGAGTTTGCC CTTCTGCCTA TAAGCTGCTG 
CTGGGAACCG AGAAGTGTGT CTGGGGCCCT AGCTACTGGT GTCAGAACAT GGAGACTGCC 
GCCCGATGCA ATGCTGTCGA TCATTGCAAA CGCCATGTGT GGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechano-Signal Transduction in Mesenchymal Stem Cells Induces Prosaposin Secretion to Drive the Proliferation of Breast Cancer Cells.
Cancer research77(22)6179-6189(2017 Nov)
Ishihara S,Inman DR,Li WJ,Ponik SM,Keely PJ

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Psap cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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