Prss12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prss12
Entrez Gene ID	19142
Full Name	protease, serine 12 neurotrypsin (motopsin)
Synonyms	Bssp-3,motopsin
General protein information	Preferred Names protease, serine 12 neurotrypsin (motopsin) Names neurotrypsin brain-specific serine protease 3 serine protease 12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G1

mRNA and Protein(s)

mRNA	Protein	Name
NM_008939.2	NP_032965.1	neurotrypsin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prss12	NP_445956.1
Bos taurus (cattle)	PRSS12	NP_001180151.1
Mus musculus (house mouse)	Prss12	NP_032965.1
Gallus gallus (chicken)	PRSS12	XP_420637.4
	PRSS12	NP_001121559.1
Xenopus tropicalis (tropical clawed frog)	prss12	XP_002934300.2
Homo sapiens (human)	PRSS12	NP_003610.2
Pan troglodytes (chimpanzee)	PRSS12	NP_001065275.1
Canis lupus familiaris (dog)	PRSS12	XP_003434056.1
Danio rerio (zebrafish)	LOC566809	XP_001920299.2

Gene Ontology
Bibliography

Related articles in PubMed

Linker proteins restore basement membrane and correct
Reinhard JR, Lin S, McKee KK, Meinen S, Crosson SC, Sury M, Hobbs S, Maier G, Yurchenco PD, R?egg MA
Science translational medicine9(396)(2017 Jun)

The C-Terminal Fragment of Agrin (CAF), a Novel Marker of Renal Function, Is Filtered by the Kidney and Reabsorbed by the Proximal Tubule.
Daryadel A, Haubitz M, Figueiredo M, Steubl D, Roos M, M?der A, Hettwer S, Wagner CA
PloS one11(7)e0157905(2016)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.
Almenar-Queralt A, Kim SN, Benner C, Herrera CM, Kang DE, Garcia-Bassets I, Goldstein LS
The Journal of biological chemistry288(49)35222-36(2013 Dec)

A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6.
Mitsui S, Hidaka C, Furihata M, Osako Y, Yuri K
Biochemical and biophysical research communications436(4)638-44(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study identifies Tequila and neurotrypsin as new genes repressed by presenilins and reveals a novel mechanism used by presenilins to modulate CREB signaling based on controlling CREB recruitment.
Title: Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

co-expression of sez-6 with motopsin restored the effect of motopsin at the basal level, while sez-6 expression alone showed no effects on cell morphology. Our results suggest that the interaction of motopsin and sez-6 modulates neuronal cell morphology
Title: A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6.

Our results indicate that an extracellular protease, motopsin, preferentially affects social behaviors, and modulates the functions of hippocampal neurons.
Title: A mental retardation gene, motopsin/neurotrypsin/prss12, modulates hippocampal function and social interaction.

Detailed analyses indicated that presynaptic action potential firing is necessary for the release of neurotrypsin, whereas postsynaptic NMDA receptor activation is necessary for the neurotrypsin-dependent cleavage of agrin.
Title: Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage.

neurotrypsin exclusively cleaves glycanated variants of agrin; neurotrypsin-dependent cleavage of agrin was concentrated at synapses, where the most heavily glycanated variants of agrin predominate
Title: Neurotrypsin cleaves agrin locally at the synapse.

The following Prss12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prss12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03128	NM_008939.2 Latest version!	Mus musculus protease, serine 12 neurotrypsin (motopsin) (Prss12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prss12 ( NM_008939.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032965.1 Mus musculus protease, serine 12 neurotrypsin (motopsin) (Prss12), mRNA.

Price & Availability↑

CloneID	OMu03128
Clone ID Related Accession (Same CDS sequence)	NM_008939.2
Accession Version	NM_008939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	neurotrypsin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110240.8 and AC124005.13. On Jun 16, 2009 this sequence version replaced NM_008939.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D89871.1, BC031429.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCTCG CCCGCTGCGT GCTGGCTGTG ATTTTAGGGG CACTGTCTGT AGTGGCCCGC 
GCTGATCCGG TCTCGCGCTC TCCCCTTCAC CGCCCGCATC CGTCCCCACC GCGTTCCCAA 
CACGCGCACT ACCTTCCCAG CTCGCGGCGG CCACCCAGGA CCCCGCGCTT CCCGCTCCCG 
CTGCGGATCC CCGCTGCCCA GCGCCCGCAG GTCCTCAGCA CCGGGCACAC GCCCCCGACG 
ATTCCACGCC GCTGCGGGGC AGGAGAGTCG TGGGGCAATG CCACCAACCT CGGCGTCCCG 
TGTCTACACT GGGACGAGGT GCCGCCCTTC CTGGAGCGGT CGCCCCCGGC CAGTTGGGCT 
GAGCTGCGAG GGCAGCCGCA CAACTTCTGC CGGAGCCCGG ATGGCTCGGG CAGACCTTGG 
TGCTTCTATC GGAATGCCCA GGGCAAAGTA GACTGGGGCT ACTGCGATTG TGGTCAAGGC 
CCGGCGTTGC CCGTCATTCG CCTTGTTGGT GGGAACAGTG GGCATGAAGG TCGAGTGGAG 
CTGTACCACG CTGGCCAGTG GGGGACCATC TGTGACGACC AATGGGACAA TGCAGACGCA 
GACGTCATCT GTAGGCAGCT GGGGCTCAGT GGCATTGCCA AAGCATGGCA TCAGGCACAT 
TTTGGGGAAG GATCTGGCCC AATATTGTTG GATGAAGTAC GCTGCACCGG AAACGAGCTG 
TCAATTGAGC AATGTCCAAA GAGTTCCTGG GGCGAACATA ACTGTGGCCA TAAAGAAGAT 
GCTGGAGTGT CTTGTGTTCC TCTAACAGAT GGTGTCATCA GACTGGCAGG AGGAAAAAGT 
ACCCATGAAG GTCGCCTGGA GGTCTACTAC AAGGGGCAGT GGGGGACAGT CTGTGATGAT 
GGCTGGACTG AGATGAACAC ATACGTGGCT TGTCGACTGC TGGGATTTAA ATACGGCAAA 
CAGTCCTCTG TGAACCATTT TGATGGCAGC AACAGGCCCA TATGGCTGGA TGACGTCAGC 
TGCTCAGGAA AAGAAGTCAG CTTCATTCAG TGTTCCAGGA GACAGTGGGG AAGGCATGAC 
TGCAGCCATA GAGAAGATGT GGGCCTCACC TGCTATCCTG ACAGCGATGG ACATAGGCTT 
TCTCCAGGTT TTCCCATCAG ACTAGTGGAT GGAGAGAATA AGAAGGAAGG ACGAGTGGAG 
GTTTTTGTCA ATGGCCAATG GGGAACAATC TGCGATGACG GATGGACCGA TAAGCATGCA 
GCTGTGATCT GCCGGCAGCT TGGCTATAAG GGTCCTGCCA GAGCAAGGAC TATGGCTTAT 
TTTGGGGAAG GAAAAGGCCC CATCCACATG GATAATGTGA AGTGCACAGG AAATGAGAAG 
GCCCTGGCTG ACTGTGTCAA ACAAGACATT GGAAGGCACA ACTGCCGCCA CAGTGAGGAT 
GCAGGAGTCA TCTGTGACTA TTTAGAGAAG AAAGCATCAA GTAGTGGTAA TAAAGAGATG 
CTCTCATCTG GATGTGGACT GAGGTTACTG CACCGTCGGC AGAAACGGAT CATTGGTGGG 
AACAATTCTT TAAGGGGTGC CTGGCCTTGG CAGGCTTCCC TCAGGCTGAG GTCGGCCCAT 
GGAGACGGCA GGCTGCTTTG TGGAGCTACC CTTCTGAGTA GCTGCTGGGT CCTGACAGCT 
GCACACTGCT TCAAAAGGTA CGGAAACAAC TCGAGGAGCT ATGCAGTTCG AGTTGGGGAT 
TATCATACTC TGGTACCAGA GGAGTTTGAA CAAGAAATAG GGGTTCAACA GATTGTGATT 
CACAGGAACT ACAGGCCAGA CAGAAGCGAC TATGACATTG CCCTGGTTAG ATTGCAAGGA 
CCAGGGGAGC AATGTGCCAG ACTAAGCACC CACGTTTTGC CAGCCTGTTT ACCTCTATGG 
AGAGAGAGGC CACAGAAAAC AGCCTCCAAC TGTCACATAA CAGGATGGGG AGACACAGGT 
CGTGCCTACT CAAGAACTCT ACAACAAGCT GCTGTGCCTC TGTTACCCAA GAGGTTTTGT 
AAAGAGAGGT ACAAGGGACT ATTTACTGGG AGAATGCTCT GTGCTGGGAA CCTCCAAGAA 
GACAACCGTG TGGACAGCTG CCAGGGAGAC AGTGGAGGAC CACTCATGTG TGAAAAGCCT 
GATGAGTCCT GGGTTGTGTA TGGGGTGACT TCCTGGGGGT ATGGATGTGG AGTCAAAGAC 
ACTCCTGGAG TTTATACCAG AGTCCCCGCC TTTGTACCTT GGATAAAAAG TGTCACCAGT 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032965.1
CDS	247..2532
Translation	MALARCVLAVILGALSVVARADPVSRSPLHRPHPSPPRSQHAHYLPSSRRPPRTPRFPLPLRIPAAQRPQVLSTGHTPPTIPRRCGAGESWGNATNLGVPCLHWDEVPPFLERSPPASWAELRGQPHNFCRSPDGSGRPWCFYRNAQGKVDWGYCDCGQGPALPVIRLVGGNSGHEGRVELYHAGQWGTICDDQWDNADADVICRQLGLSGIAKAWHQAHFGEGSGPILLDEVRCTGNELSIEQCPKSSWGEHNCGHKEDAGVSCVPLTDGVIRLAGGKSTHEGRLEVYYKGQWGTVCDDGWTEMNTYVACRLLGFKYGKQSSVNHFDGSNRPIWLDDVSCSGKEVSFIQCSRRQWGRHDCSHREDVGLTCYPDSDGHRLSPGFPIRLVDGENKKEGRVEVFVNGQWGTICDDGWTDKHAAVICRQLGYKGPARARTMAYFGEGKGPIHMDNVKCTGNEKALADCVKQDIGRHNCRHSEDAGVICDYLEKKASSSGNKEMLSSGCGLRLLHRRQKRIIGGNNSLRGAWPWQASLRLRSAHGDGRLLCGATLLSSCWVLTAAHCFKRYGNNSRSYAVRVGDYHTLVPEEFEQEIGVQQIVIHRNYRPDRSDYDIALVRLQGPGEQCARLSTHVLPACLPLWRERPQKTASNCHITGWGDTGRAYSRTLQQAAVPLLPKRFCKERYKGLFTGRMLCAGNLQEDNRVDSCQGDSGGPLMCEKPDESWVVYGVTSWGYGCGVKDTPGVYTRVPAFVPWIKSVTSL

Target ORF information:

RefSeq Version	NM_008939.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 12 neurotrypsin (motopsin) (Prss12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008939.2

ORF Insert Sequence:

ATGGCGCTCG CCCGCTGCGT GCTGGCTGTG ATTTTAGGGG CACTGTCTGT AGTGGCCCGC 
GCTGATCCGG TCTCGCGCTC TCCCCTTCAC CGCCCGCATC CGTCCCCACC GCGTTCCCAA 
CACGCGCACT ACCTTCCCAG CTCGCGGCGG CCACCCAGGA CCCCGCGCTT CCCGCTCCCG 
CTGCGGATCC CCGCTGCCCA GCGCCCGCAG GTCCTCAGCA CCGGGCACAC GCCCCCGACG 
ATTCCACGCC GCTGCGGGGC AGGAGAGTCG TGGGGCAATG CCACCAACCT CGGCGTCCCG 
TGTCTACACT GGGACGAGGT GCCGCCCTTC CTGGAGCGGT CGCCCCCGGC CAGTTGGGCT 
GAGCTGCGAG GGCAGCCGCA CAACTTCTGC CGGAGCCCGG ATGGCTCGGG CAGACCTTGG 
TGCTTCTATC GGAATGCCCA GGGCAAAGTA GACTGGGGCT ACTGCGATTG TGGTCAAGGC 
CCGGCGTTGC CCGTCATTCG CCTTGTTGGT GGGAACAGTG GGCATGAAGG TCGAGTGGAG 
CTGTACCACG CTGGCCAGTG GGGGACCATC TGTGACGACC AATGGGACAA TGCAGACGCA 
GACGTCATCT GTAGGCAGCT GGGGCTCAGT GGCATTGCCA AAGCATGGCA TCAGGCACAT 
TTTGGGGAAG GATCTGGCCC AATATTGTTG GATGAAGTAC GCTGCACCGG AAACGAGCTG 
TCAATTGAGC AATGTCCAAA GAGTTCCTGG GGCGAACATA ACTGTGGCCA TAAAGAAGAT 
GCTGGAGTGT CTTGTGTTCC TCTAACAGAT GGTGTCATCA GACTGGCAGG AGGAAAAAGT 
ACCCATGAAG GTCGCCTGGA GGTCTACTAC AAGGGGCAGT GGGGGACAGT CTGTGATGAT 
GGCTGGACTG AGATGAACAC ATACGTGGCT TGTCGACTGC TGGGATTTAA ATACGGCAAA 
CAGTCCTCTG TGAACCATTT TGATGGCAGC AACAGGCCCA TATGGCTGGA TGACGTCAGC 
TGCTCAGGAA AAGAAGTCAG CTTCATTCAG TGTTCCAGGA GACAGTGGGG AAGGCATGAC 
TGCAGCCATA GAGAAGATGT GGGCCTCACC TGCTATCCTG ACAGCGATGG ACATAGGCTT 
TCTCCAGGTT TTCCCATCAG ACTAGTGGAT GGAGAGAATA AGAAGGAAGG ACGAGTGGAG 
GTTTTTGTCA ATGGCCAATG GGGAACAATC TGCGATGACG GATGGACCGA TAAGCATGCA 
GCTGTGATCT GCCGGCAGCT TGGCTATAAG GGTCCTGCCA GAGCAAGGAC TATGGCTTAT 
TTTGGGGAAG GAAAAGGCCC CATCCACATG GATAATGTGA AGTGCACAGG AAATGAGAAG 
GCCCTGGCTG ACTGTGTCAA ACAAGACATT GGAAGGCACA ACTGCCGCCA CAGTGAGGAT 
GCAGGAGTCA TCTGTGACTA TTTAGAGAAG AAAGCATCAA GTAGTGGTAA TAAAGAGATG 
CTCTCATCTG GATGTGGACT GAGGTTACTG CACCGTCGGC AGAAACGGAT CATTGGTGGG 
AACAATTCTT TAAGGGGTGC CTGGCCTTGG CAGGCTTCCC TCAGGCTGAG GTCGGCCCAT 
GGAGACGGCA GGCTGCTTTG TGGAGCTACC CTTCTGAGTA GCTGCTGGGT CCTGACAGCT 
GCACACTGCT TCAAAAGGTA CGGAAACAAC TCGAGGAGCT ATGCAGTTCG AGTTGGGGAT 
TATCATACTC TGGTACCAGA GGAGTTTGAA CAAGAAATAG GGGTTCAACA GATTGTGATT 
CACAGGAACT ACAGGCCAGA CAGAAGCGAC TATGACATTG CCCTGGTTAG ATTGCAAGGA 
CCAGGGGAGC AATGTGCCAG ACTAAGCACC CACGTTTTGC CAGCCTGTTT ACCTCTATGG 
AGAGAGAGGC CACAGAAAAC AGCCTCCAAC TGTCACATAA CAGGATGGGG AGACACAGGT 
CGTGCCTACT CAAGAACTCT ACAACAAGCT GCTGTGCCTC TGTTACCCAA GAGGTTTTGT 
AAAGAGAGGT ACAAGGGACT ATTTACTGGG AGAATGCTCT GTGCTGGGAA CCTCCAAGAA 
GACAACCGTG TGGACAGCTG CCAGGGAGAC AGTGGAGGAC CACTCATGTG TGAAAAGCCT 
GATGAGTCCT GGGTTGTGTA TGGGGTGACT TCCTGGGGGT ATGGATGTGG AGTCAAAGAC 
ACTCCTGGAG TTTATACCAG AGTCCCCGCC TTTGTACCTT GGATAAAAAG TGTCACCAGT 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Linker proteins restore basement membrane and correct
Science translational medicine9(396)(2017 Jun)
Reinhard JR,Lin S,McKee KK,Meinen S,Crosson SC,Sury M,Hobbs S,Maier G,Yurchenco PD,R?egg MA

The C-Terminal Fragment of Agrin (CAF), a Novel Marker of Renal Function, Is Filtered by the Kidney and Reabsorbed by the Proximal Tubule.
PloS one11(7)e0157905(2016)
Daryadel A,Haubitz M,Figueiredo M,Steubl D,Roos M,M?der A,Hettwer S,Wagner CA

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.
The Journal of biological chemistry288(49)35222-36(2013 Dec)
Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6.
Biochemical and biophysical research communications436(4)638-44(2013 Jul)
Mitsui S,Hidaka C,Furihata M,Osako Y,Yuri K

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Prss12 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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