Pla2g2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pla2g2a
Entrez Gene ID	18780
Full Name	phospholipase A2, group IIA (platelets, synovial fluid)
Synonyms	EF,Mom1,Pla2,sPLA2,sPla2-IIA
General protein information	Preferred Names phospholipase A2, group IIA (platelets, synovial fluid) Names phospholipase A2, membrane associated GIIC sPLA2 enhancing factor group IIA phospholipase A2 modifier of Min1 non-pancreatic secreted type II phospholipase A2 phosphatidylcholine 2-acylhydrolase 2A secretory group II phospholipase A2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D3\|4 70.57 cM
Summary	Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082531.1	NP_001076000.1	phospholipase A2, membrane associated precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cytosolic Phospholipase A
Bhowmick R, Clark S, Bonventre JV, Leong JM, McCormick BA
Infection and immunity85(11)(2017 Nov)

sPLA2 -IIA Overexpression in Mice Epidermis Depletes Hair Follicle Stem Cells and Induces Differentiation Mediated Through Enhanced JNK/c-Jun Activation.
Sarate RM, Chovatiya GL, Ravi V, Khade B, Gupta S, Waghmare SK
Stem cells (Dayton, Ohio)34(9)2407-17(2016 09)

Secreted Phospholipases A2 Are Intestinal Stem Cell Niche Factors with Distinct Roles in Homeostasis, Inflammation, and Cancer.
Schewe M, Franken PF, Sacchetti A, Schmitt M, Joosten R, B?ttcher R, van Royen ME, Jeammet L, Payr? C, Scott PM, Webb NR, Gelb M, Cormier RT, Lambeau G, Fodde R
Cell stem cell19(1)38-51(2016 Jul)

Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase.
Joshi V, Umashankara M, Ramakrishnan C, Nanjaraj Urs AN, Suvilesh KN, Velmurugan D, Rangappa KS, Vishwanath BS
Archives of biochemistry and biophysics59828-39(2016 May)

Paneth cell differentiation in the developing intestine of normal and transgenic mice.
Bry L, Falk P, Huttner K, Ouellette A, Midtvedt T, Gordon JI
Proceedings of the National Academy of Sciences of the United States of America91(22)10335-9(1994 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLA2 promotes pulmonary inflammation and systemic disease during Streptococcus pneumoniae infection.
Title: Cytosolic Phospholipase A

transgenic mice overexpressing sPLA2 -IIA keratinocytes showed enhanced activation of EGFR and JNK1/2 that led to c-Jun activation.
Title: sPLA2 -IIA Overexpression in Mice Epidermis Depletes Hair Follicle Stem Cells and Induces Differentiation Mediated Through Enhanced JNK/c-Jun Activation.

secretory PLA2s have important functions as genetic modifiers of inflammation and colon cancer.
Title: Secreted Phospholipases A2 Are Intestinal Stem Cell Niche Factors with Distinct Roles in Homeostasis, Inflammation, and Cancer.

Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase.
Title: Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase.

Polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases.
Title: Inhibitory effect of polyozellin on secretory group IIA phospholipase A2.

The following Pla2g2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pla2g2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18755	NM_001082531.1 Latest version!	Mus musculus phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), transcript variant 1, coding, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pla2g2a ( NM_001082531.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001076000.1 Mus musculus phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), transcript variant 1, coding, mRNA.

Price & Availability↑

CloneID	OMu18755
Clone ID Related Accession (Same CDS sequence)	NM_001082531.1
Accession Version	NM_001082531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	phospholipase A2, membrane associated precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC045156.1. Transcript Variant: This variant (1, coding) contains a gap in the coding region to represent the functional allele that is not present in the the strain of the reference genome, C57BL/6J. Sequence Note: This RefSeq record was created to represent the transcript and the full-length, functional protein that is expressed in several strains. It does not contain a one nucleotide deletion that causes a frameshift in the CDS of several strains, including the strain of the reference genome, C57BL/6J. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045156.1, X74266.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: PMID: 7673223 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTCC TCCTGCTGCT AGCAGCCTCG ATCATGGCCT TTGGCTCAAT ACAGGTCCAA 
GGGAACATTG CGCAGTTTGG GGAAATGATT CGGCTTAAGA CAGGAAAGAG AGCTGAGCTT 
AGCTATGCCT TCTATGGATG CCACTGTGGC CTGGGTGGCA AAGGATCCCC CAAGGATGCC 
ACAGACCGGT GCTGTGTTAC TCATGACTGT TGCTACAAGA GCCTGGAGAA AAGTGGATGT 
GGTACTAAGT TACTGAAATA CAAGTACTCC CACCAAGGGG GCCAAATCAC CTGTTCTGCA 
AACCAGAACT CCTGTCAGAA ACGGCTGTGT CAGTGCGATA AAGCCGCCGC TGAATGTTTC 
GCCCGGAACA AGAAAACCTA CAGTTTAAAG TACCAGTTCT ACCCCAACAT GTTTTGCAAA 
GGGAAGAAGC CCAAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076000.1
CDS	37..477
Translation	MKVLLLLAASIMAFGSIQVQGNIAQFGEMIRLKTGKRAELSYAFYGCHCGLGGKGSPKDATDRCCVTHDCCYKSLEKSGCGTKLLKYKYSHQGGQITCSANQNSCQKRLCQCDKAAAECFARNKKTYSLKYQFYPNMFCKGKKPKC

Target ORF information:

RefSeq Version	NM_001082531.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase A2, group IIA (platelets, synovial fluid) (Pla2g2a), transcript variant 1, coding, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082531.1

ORF Insert Sequence:

ATGAAGGTCC TCCTGCTGCT AGCAGCCTCG ATCATGGCCT TTGGCTCAAT ACAGGTCCAA 
GGGAACATTG CGCAGTTTGG GGAAATGATT CGGCTTAAGA CAGGAAAGAG AGCTGAGCTT 
AGCTATGCCT TCTATGGATG CCACTGTGGC CTGGGTGGCA AAGGATCCCC CAAGGATGCC 
ACAGACCGGT GCTGTGTTAC TCATGACTGT TGCTACAAGA GCCTGGAGAA AAGTGGATGT 
GGTACTAAGT TACTGAAATA CAAGTACTCC CACCAAGGGG GCCAAATCAC CTGTTCTGCA 
AACCAGAACT CCTGTCAGAA ACGGCTGTGT CAGTGCGATA AAGCCGCCGC TGAATGTTTC 
GCCCGGAACA AGAAAACCTA CAGTTTAAAG TACCAGTTCT ACCCCAACAT GTTTTGCAAA 
GGGAAGAAGC CCAAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytosolic Phospholipase A
Infection and immunity85(11)(2017 Nov)
Bhowmick R,Clark S,Bonventre JV,Leong JM,McCormick BA

sPLA2 -IIA Overexpression in Mice Epidermis Depletes Hair Follicle Stem Cells and Induces Differentiation Mediated Through Enhanced JNK/c-Jun Activation.
Stem cells (Dayton, Ohio)34(9)2407-17(2016 09)
Sarate RM,Chovatiya GL,Ravi V,Khade B,Gupta S,Waghmare SK

Secreted Phospholipases A2 Are Intestinal Stem Cell Niche Factors with Distinct Roles in Homeostasis, Inflammation, and Cancer.
Cell stem cell19(1)38-51(2016 Jul)
Schewe M,Franken PF,Sacchetti A,Schmitt M,Joosten R,B?ttcher R,van Royen ME,Jeammet L,Payr? C,Scott PM,Webb NR,Gelb M,Cormier RT,Lambeau G,Fodde R

Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase.
Archives of biochemistry and biophysics59828-39(2016 May)
Joshi V,Umashankara M,Ramakrishnan C,Nanjaraj Urs AN,Suvilesh KN,Velmurugan D,Rangappa KS,Vishwanath BS

Paneth cell differentiation in the developing intestine of normal and transgenic mice.
Proceedings of the National Academy of Sciences of the United States of America91(22)10335-9(1994 Oct)
Bry L,Falk P,Huttner K,Ouellette A,Midtvedt T,Gordon JI

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Pla2g2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報