Pcsk2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcsk2
Entrez Gene ID	18549
Full Name	proprotein convertase subtilisin/kexin type 2
Synonyms	6330411F23Rik,AI839700,Nec-2,Nec2,PC2,Phpp-2,SPC2
General protein information	Preferred Names proprotein convertase subtilisin/kexin type 2 Names neuroendocrine convertase 2 KEX2-like endoprotease 2 NEC 2 prohormone convertase 2 proprotein convertase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 G1\|2 70.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008792.4	NP_032818.1	neuroendocrine convertase 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCSK2	NP_002585.2
Danio rerio (zebrafish)	pcsk2	NP_001135738.1
Drosophila melanogaster (fruit fly)	amon	NP_477318.1
Xenopus tropicalis (tropical clawed frog)	pcsk2	NP_001090660.1
Pan troglodytes (chimpanzee)	PCSK2	XP_001138346.1
	PCSK2	XP_001087532.1
Rattus norvegicus (Norway rat)	Pcsk2	NP_036878.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002176	XP_308012.5
Canis lupus familiaris (dog)	PCSK2	XP_003433232.1
Bos taurus (cattle)	PCSK2	NP_776838.1
Caenorhabditis elegans (roundworm)	egl-3	NP_001023732.1
Mus musculus (house mouse)	Pcsk2	NP_032818.1
Gallus gallus (chicken)	PCSK2	XP_004940271.1

Gene Ontology
Bibliography

Related articles in PubMed

Targeted high-resolution quadrupole-Orbitrap mass spectrometry analyses reveal a significant reduction of tachykinin and opioid neuropeptides level in PC1 and PC2 mutant mouse spinal cords.
Saidi M, Beaudry F
Neuropeptides6537-44(2017 Oct)

Loss of prohormone convertase 2 promotes beta cell dysfunction in a rodent transplant model expressing human pro-islet amyloid polypeptide.
Courtade JA, Wang EY, Yen P, Dai DL, Soukhatcheva G, Orban PC, Verchere CB
Diabetologia60(3)453-463(2017 Mar)

Prohormone convertase 2 (PC2) null mice have increased mu opioid receptor levels accompanied by altered morphine-induced antinociception, tolerance and dependence.
Lutfy K, Parikh D, Lee DL, Liu Y, Ferrini MG, Hamid A, Friedman TC
Neuroscience329318-25(2016 08)

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
Abdellatif AM, Oishi H, Itagaki T, Jung Y, Shawki HH, Okita Y, Hasegawa Y, Suzuki H, El-Morsy SE, El-Sayed MA, Shoaib MB, Sugiyama F, Takahashi S
PloS one11(2)e0150010(2016)

The MAFB transcription factor impacts islet ?-cell function in rodents and represents a unique signature of primate islet ?-cells.
Conrad E, Dai C, Spaeth J, Guo M, Cyphert HA, Scoville D, Carroll J, Yu WM, Goodrich LV, Harlan DM, Grove KL, Roberts CT Jr, Powers AC, Gu G, Stein R
American journal of physiology. Endocrinology and metabolism310(1)E91-E102(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports that PC2 null mice had increased number of mu opioid receptors in different brain areas. These mice also exhibited enhanced morphine-induced antinociception but reduced tolerance to and dependence on morphine.
Title: Prohormone convertase 2 (PC2) null mice have increased mu opioid receptor levels accompanied by altered morphine-induced antinociception, tolerance and dependence.

PC1 and PC2 are involved in the C-terminal processing of protachykinin peptides and suggest a major role in the maturation of the protachykinin-1 protein
Title: Tachykinins Processing is Significantly Impaired in PC1 and PC2 Mutant Mouse Spinal Cord S9 Fractions.

activation of the hypothalamic GPR40/FFA1 signaling pathway may regulate beta-endorphin release via PC2, and regulate the endogenous pain control system
Title: Regulation of prohormone convertase 2 protein expression via GPR40/FFA1 in the hypothalamus.

Verified the roles of proprotein convertase subtilisin/kexin type 2 in regulating IGF1 and female sexual maturation, it also might be involved in the regulation of aging.
Title: Genetic Regulation of Female Sexual Maturation and Longevity Through Circulating IGF1.

Islets of Langerhans from prohormone convertase-2 knockout mice show alpha-cell hyperplasia and tumorigenesis with elevated alpha-cell neogenesis.
Title: Islets of Langerhans from prohormone convertase-2 knockout mice show ?-cell hyperplasia and tumorigenesis with elevated ?-cell neogenesis.

The following Pcsk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcsk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16133	NM_008792.4 Latest version!	Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcsk2 ( NM_008792.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032818.1 Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2), mRNA.

Price & Availability↑

CloneID	OMu16133
Clone ID Related Accession (Same CDS sequence)	NM_008792.4
Accession Version	NM_008792.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	neuroendocrine convertase 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158598.1, BC057348.1 and AL807801.3. On Apr 22, 2009 this sequence version replaced NM_008792.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057348.1, SRR1660813.253941.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849389 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG GTTGTGGATC CCAGTGGAAG GCGGCCGGGT TCCTCTTCTG TGTGATGGTT 
TTTGCGTCTG CCGAGAGACC CGTCTTCACG AATCATTTTC TTGTGGAGTT GCATAAAGAC 
GGAGAGGAAG AGGCTCGCCA AGTTGCAGCA GAACACGGCT TTGGAGTCCG AAAGCTCCCC 
TTTGCAGAAG GCCTGTATCA CTTTTATCAC AATGGGCTTG CAAAGGCCAA AAGAAGACGC 
AGCCTACACC ATAAGCGGCA GCTAGAGAGA GACCCCAGGA TAAAGATGGC GCTGCAACAA 
GAAGGATTTG ACCGTAAAAA GAGAGGGTAC AGGGACATCA ATGAGATTGA CATCAACATG 
AATGATCCTC TCTTTACAAA GCAATGGTAC CTGTTCAACA CTGGGCAAGC CGATGGAACT 
CCTGGGCTAG ACTTGAACGT GGCCGAAGCC TGGGAGCTGG GATACACAGG AAAAGGAGTG 
ACCATTGGAA TTATGGATGA TGGAATTGAC TATCTCCACC CAGACCTGGC CTACAACTAC 
AACGCTGATG CAAGTTATGA CTTCAGCAGC AATGACCCCT ACCCATACCC TCGATACACA 
GATGACTGGT TCAACAGCCA TGGAACTAGG TGTGCAGGAG AAGTTTCTGC TGCAGCCAGC 
AACAATATCT GTGGAGTCGG CGTAGCATAC AACTCCAAGG TGGCAGGGAT CCGGATGCTG 
GACCAGCCCT TTATGACAGA CATCATCGAA GCCTCCTCCA TCAGCCACAT GCCTCAACTG 
ATCGACATCT ACAGTGCAAG CTGGGGCCCC ACAGACAATG GGAAGACGGT TGATGGGCCC 
CGAGAGCTCA CACTCCAGGC CATGGCTGAT GGCGTGAACA AGGGCCGTGG GGGCAAAGGC 
AGCATCTATG TGTGGGCCTC TGGGGACGGT GGCAGCTACG ATGACTGCAA CTGTGACGGC 
TATGCTTCAA GCATGTGGAC CATCTCCATC AACTCAGCCA TCAATGATGG CAGGACTGCC 
TTGTATGATG AGAGTTGCTC TTCCACCTTA GCATCCACCT TCAGCAATGG GAGGAAGAGG 
AATCCTGAGG CTGGTGTGGC TACCACAGAC TTGTATGGCA ACTGTACTCT GAGACACTCT 
GGGACATCTG CAGCTGCTCC GGAGGCAGCT GGCGTGTTTG CATTAGCTTT GGAGGCTAAC 
CTGGATCTGA CCTGGAGAGA CATGCAACAT CTGACTGTGC TCACCTCCAA GCGGAACCAG 
CTTCATGATG AGGTTCATCA GTGGCGACGG AATGGGGTTG GCCTGGAATT TAATCACCTC 
TTTGGCTACG GAGTCCTTGA TGCAGGTGCC ATGGTGAAAA TGGCTAAAGA CTGGAAAACT 
GTTCCGGAGA GATTCCATTG TGTGGGAGGC TCTGTGCAGA ACCCTGAAAA AATACCACCC 
ACCGGCAAGC TGGTACTGAC CCTCAAAACA AATGCATGTG AGGGGAAGGA AAACTTCGTC 
CGCTACCTGG AGCACGTCCA AGCTGTCATC ACAGTCAACG CGACCAGGAG AGGAGACCTG 
AACATCAACA TGACCTCCCC AATGGGCACC AAGTCCATTT TGCTGAGCCG GCGTCCCAGA 
GACGACGACT CCAAGGTGGG CTTTGACAAG TGGCCTTTCA TGACCACCCA CACCTGGGGG 
GAGGATGCCC GAGGGACCTG GACCCTGGAG CTGGGGTTTG TGGGCAGTGC ACCACAGAAG 
GGGTTGCTGA AGGAATGGAC CCTGATGCTT CACGGCACAC AGAGCGCCCC ATACATCGAT 
CAGGTGGTGA GGGATTACCA GTCTAAGCTG GCCATGTCCA AGAAGCAGGA GCTGGAGGAA 
GAGCTGGATG AGGCTGTGGA GAGAAGCCTG CAAAGTATCC TGAGAAAGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032818.1
CDS	359..2272
Translation	MEGGCGSQWKAAGFLFCVMVFASAERPVFTNHFLVELHKDGEEEARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKRQLERDPRIKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLFNTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLAYNYNADASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAASNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLDLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQNPEKIPPTGKLVLTLKTNACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGLLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKQELEEELDEAVERSLQSILRKN

Target ORF information:

RefSeq Version	NM_008792.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008792.4

ORF Insert Sequence:

ATGGAGGGCG GTTGTGGATC CCAGTGGAAG GCGGCCGGGT TCCTCTTCTG TGTGATGGTT 
TTTGCGTCTG CCGAGAGACC CGTCTTCACG AATCATTTTC TTGTGGAGTT GCATAAAGAC 
GGAGAGGAAG AGGCTCGCCA AGTTGCAGCA GAACACGGCT TTGGAGTCCG AAAGCTCCCC 
TTTGCAGAAG GCCTGTATCA CTTTTATCAC AATGGGCTTG CAAAGGCCAA AAGAAGACGC 
AGCCTACACC ATAAGCGGCA GCTAGAGAGA GACCCCAGGA TAAAGATGGC GCTGCAACAA 
GAAGGATTTG ACCGTAAAAA GAGAGGGTAC AGGGACATCA ATGAGATTGA CATCAACATG 
AATGATCCTC TCTTTACAAA GCAATGGTAC CTGTTCAACA CTGGGCAAGC CGATGGAACT 
CCTGGGCTAG ACTTGAACGT GGCCGAAGCC TGGGAGCTGG GATACACAGG AAAAGGAGTG 
ACCATTGGAA TTATGGATGA TGGAATTGAC TATCTCCACC CAGACCTGGC CTACAACTAC 
AACGCTGATG CAAGTTATGA CTTCAGCAGC AATGACCCCT ACCCATACCC TCGATACACA 
GATGACTGGT TCAACAGCCA TGGAACTAGG TGTGCAGGAG AAGTTTCTGC TGCAGCCAGC 
AACAATATCT GTGGAGTCGG CGTAGCATAC AACTCCAAGG TGGCAGGGAT CCGGATGCTG 
GACCAGCCCT TTATGACAGA CATCATCGAA GCCTCCTCCA TCAGCCACAT GCCTCAACTG 
ATCGACATCT ACAGTGCAAG CTGGGGCCCC ACAGACAATG GGAAGACGGT TGATGGGCCC 
CGAGAGCTCA CACTCCAGGC CATGGCTGAT GGCGTGAACA AGGGCCGTGG GGGCAAAGGC 
AGCATCTATG TGTGGGCCTC TGGGGACGGT GGCAGCTACG ATGACTGCAA CTGTGACGGC 
TATGCTTCAA GCATGTGGAC CATCTCCATC AACTCAGCCA TCAATGATGG CAGGACTGCC 
TTGTATGATG AGAGTTGCTC TTCCACCTTA GCATCCACCT TCAGCAATGG GAGGAAGAGG 
AATCCTGAGG CTGGTGTGGC TACCACAGAC TTGTATGGCA ACTGTACTCT GAGACACTCT 
GGGACATCTG CAGCTGCTCC GGAGGCAGCT GGCGTGTTTG CATTAGCTTT GGAGGCTAAC 
CTGGATCTGA CCTGGAGAGA CATGCAACAT CTGACTGTGC TCACCTCCAA GCGGAACCAG 
CTTCATGATG AGGTTCATCA GTGGCGACGG AATGGGGTTG GCCTGGAATT TAATCACCTC 
TTTGGCTACG GAGTCCTTGA TGCAGGTGCC ATGGTGAAAA TGGCTAAAGA CTGGAAAACT 
GTTCCGGAGA GATTCCATTG TGTGGGAGGC TCTGTGCAGA ACCCTGAAAA AATACCACCC 
ACCGGCAAGC TGGTACTGAC CCTCAAAACA AATGCATGTG AGGGGAAGGA AAACTTCGTC 
CGCTACCTGG AGCACGTCCA AGCTGTCATC ACAGTCAACG CGACCAGGAG AGGAGACCTG 
AACATCAACA TGACCTCCCC AATGGGCACC AAGTCCATTT TGCTGAGCCG GCGTCCCAGA 
GACGACGACT CCAAGGTGGG CTTTGACAAG TGGCCTTTCA TGACCACCCA CACCTGGGGG 
GAGGATGCCC GAGGGACCTG GACCCTGGAG CTGGGGTTTG TGGGCAGTGC ACCACAGAAG 
GGGTTGCTGA AGGAATGGAC CCTGATGCTT CACGGCACAC AGAGCGCCCC ATACATCGAT 
CAGGTGGTGA GGGATTACCA GTCTAAGCTG GCCATGTCCA AGAAGCAGGA GCTGGAGGAA 
GAGCTGGATG AGGCTGTGGA GAGAAGCCTG CAAAGTATCC TGAGAAAGAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeted high-resolution quadrupole-Orbitrap mass spectrometry analyses reveal a significant reduction of tachykinin and opioid neuropeptides level in PC1 and PC2 mutant mouse spinal cords.
Neuropeptides6537-44(2017 Oct)
Saidi M,Beaudry F

Loss of prohormone convertase 2 promotes beta cell dysfunction in a rodent transplant model expressing human pro-islet amyloid polypeptide.
Diabetologia60(3)453-463(2017 Mar)
Courtade JA,Wang EY,Yen P,Dai DL,Soukhatcheva G,Orban PC,Verchere CB

Prohormone convertase 2 (PC2) null mice have increased mu opioid receptor levels accompanied by altered morphine-induced antinociception, tolerance and dependence.
Neuroscience329318-25(2016 08)
Lutfy K,Parikh D,Lee DL,Liu Y,Ferrini MG,Hamid A,Friedman TC

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
PloS one11(2)e0150010(2016)
Abdellatif AM,Oishi H,Itagaki T,Jung Y,Shawki HH,Okita Y,Hasegawa Y,Suzuki H,El-Morsy SE,El-Sayed MA,Shoaib MB,Sugiyama F,Takahashi S

The MAFB transcription factor impacts islet ?-cell function in rodents and represents a unique signature of primate islet ?-cells.
American journal of physiology. Endocrinology and metabolism310(1)E91-E102(2016 Jan)
Conrad E,Dai C,Spaeth J,Guo M,Cyphert HA,Scoville D,Carroll J,Yu WM,Goodrich LV,Harlan DM,Grove KL,Roberts CT Jr,Powers AC,Gu G,Stein R

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Pcsk2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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