Nedd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nedd1
Entrez Gene ID	17997
Full Name	neural precursor cell expressed, developmentally down-regulated gene 1
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated gene 1 Names protein NEDD1 NEDD-1 neural precursor cell expressed developmentally down-regulated protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513334.1	XP_006513397.1	protein NEDD1 isoform X1
NM_008682.2	NP_032708.2	protein NEDD1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEDD1	NP_001128647.1
Bos taurus (cattle)	NEDD1	NP_001030410.1
Mus musculus (house mouse)	Nedd1	NP_032708.2
Rattus norvegicus (Norway rat)	Nedd1	NP_001100249.1
Pan troglodytes (chimpanzee)	NEDD1	XP_001148080.1
	NEDD1	XP_001108208.2
Xenopus tropicalis (tropical clawed frog)	nedd1	NP_001072456.2
Canis lupus familiaris (dog)	NEDD1	XP_005629313.1
Gallus gallus (chicken)	NEDD1	NP_001025921.1
Danio rerio (zebrafish)	nedd1	NP_998671.1

Gene Ontology
Bibliography

Related articles in PubMed

Divergent regulation of functionally distinct ?-tubulin complexes during differentiation.
Muroyama A, Seldin L, Lechler T
The Journal of cell biology213(6)679-92(2016 Jun)

CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination.
Marjanovi? M, S?nchez-Huertas C, Terr? B, G?mez R, Scheel JF, Pacheco S, Knobel PA, Mart?nez-Marchal A, Aivio S, Palenzuela L, Wolfrum U, McKinnon PJ, Suja JA, Roig I, Costanzo V, L?ders J, Stracker TH
Nature communications67676(2015 Jul)

Intraperitoneal delivery of a small interfering RNA targeting NEDD1 prolongs the survival of scirrhous gastric cancer model mice.
Fujita T, Yanagihara K, Takeshita F, Aoyagi K, Nishimura T, Takigahira M, Chiwaki F, Fukagawa T, Katai H, Ochiya T, Sakamoto H, Konno H, Yoshida T, Sasaki H
Cancer science104(2)214-22(2013 Feb)

Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip.
Machold R, Klein C, Fishell G
Gene expression patterns : GEP11(5-6)349-59(2011 Jun-Jul)

A potential role for NEDD1 and the centrosome in senescence of mouse embryonic fibroblasts.
Manning JA, Kumar S
Cell death & disease1e35(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mice treated with small interfering (si) RNA targeting NEDD1 by the atelocollagen-mediated delivery system showed a significantly prolonged survival.
Title: Intraperitoneal delivery of a small interfering RNA targeting NEDD1 prolongs the survival of scirrhous gastric cancer model mice.

Disrupting the centrosome in early-passage mouse embryonic fibroblasts by depletion of NEDD1 also resulted in centrosomal fragmentation and subsequent premature entry into senescence.
Title: A potential role for NEDD1 and the centrosome in senescence of mouse embryonic fibroblasts.

a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome
Title: A direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome.

NEDD1 is an essential component of acentriolar oocyte MTOCs, which functions in the regulation of meiotic spindle stability
Title: NEDD1 is crucial for meiotic spindle stability and accurate chromosome segregation in mammalian oocytes.

the localization of Nedd1 and the centrosome during important processes in mouse embryogenesis and provides a basis for further study into its role in development.
Title: Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development.

The following Nedd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu44283	XM_006513334.1 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu02524	NM_008682.2 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$384.30 $549.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd1 ( XM_006513334.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513397.1 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44283
Clone ID Related Accession (Same CDS sequence)	XM_006513334.1
Accession Version	XM_006513334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein NEDD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGCG GGACTTCCGG CCGCCCAAGT TTAACAGTCC AGGCGGGAGC CGGAAGCGCA 
GCGAGGAACG GGTGGAGCTG GGCCTCCCCT GTGGCCCCAC GCTTGAGAGG CCCAGTCATG 
CAGGAAAACC TCAGATTTGC TTCATCAGGA GATGACGTTA AAATATGGGA TGCTTCGTTC 
CTGACATTGG TGGATAAATT CAACCCTCAC ACGTCACCGC ATGGAATCAG TTCAATCTGT 
TGGAGCAGCA ATAATAACTT TCTAGTGACA GCTTCTTCCA GTGGTGACAA AATAGTGGTG 
TCAAGTTGCA AATGTAAGCC CGTTCCACTT TTGGAGCTTG CGGAAGGGCA AAAGCAGACA 
TGTGTCGATT TAAATTCTAC ATCAATGTAT TTGGCAAGTG GAGGCCTAAA TAACACTGTT 
AATATTTGGG ATTTAAAGTC CAAACGACTT CATCGGTCTC TTAAGGATCA TAAATGTGAA 
GTAACTTGTG TGGCATACAA TTGGAATGAC TGTTACATTG CTTCTGGGTC TCTCAGTGGT 
GAGATTATCT TACACAGTGT AACCACTAAT ACATCCAGCA CACCTTTTGG CCATGGGAGT 
AAGCAGCCTA TCCGGCACAT CAAGTACTCA TTGTTTAGGA AGTCTCTCTT GGGCAGCGTT 
TCGGATAATG GAGTAGTGAC TCTCTGGGAT GTGAACAGTC AGAGTTCATA CCACACCTTT 
GACAGTACAC ACAAAGCCCC AGCTTCAGGC ATCTGCTTCT CTCCTGTCAA TGAACTGCTG 
TTTGTAACCA TAGGCCTGGA TAAAAGAATC ATCCTCTATG ATACTTCAAG TAAGAAGCTA 
GTGAAAACTT TAGTGGCCGA CACCCCCCTG ACTGCAGTAG ATTTTATGCC GGATGGAGCC 
ACTTTGGCTA TTGGATCTTC CCGTGGGAAA ATATATCAGT ATGACTTAAG GATGCTGAAA 
TCTCCCGTGA AAACCATCAG TGCCCACAAG ACATCTGTGC AGTGTATAGC ATTTCAGTAC 
TCCACATCTC TGACGAAGGC AAGTTTAAGC AAAGGCAGCT CAAATAAGGC CACAGCAGTG 
AACAAACGGA GTGTTCCTGT GAGCAGCAGC AGTGGGGCTG CCCAGAATTC TGGAATTGTC 
AGAGAAGCAC CCAGCCCTTC CATTGCCACA GTTCTCCCAC AGCCCGTGAC AACAGCTCTG 
GGCAAAGGAT CAGGGGCTGC TCAGGACGAA GCAGGTTTGG CTCGAAGCAA AAGTACAGAT 
ATTTTCTCTA AGGAAACAGA TGCTGGAAAA AGTCAGGATT TCTCCAGCTT TGATGATACT 
GGGAAAAATA GTTTAGGTGA CATGTTCTCA CCAATCAGAG ATGATGCTGT AGTCAGCAAA 
GGAGGTGATG AATCTATAGG CAAAGGAGAT GGCCTTGACT TTCTACCACA GCTAAACTCA 
GTGTTCCCTC TGAGGAAGAA TGCAGGTGCC TCCAGCAGTC TGGTGCTGCA CTCCAGCCCT 
CTTAATGTCC TCATGGGATC TTCAGGGAAA GAAGAGAATG AAAGCCATGA GCCGTCAGCT 
GAGTCTAAAA GAGCATACCT AGGGAAGCAG GAACCTAAGG ACGCCATGAA GCAGTTTACA 
AAGTTGATCT CCTCTGGTGC TGAACCTGGA ATTCTAAATA CCTGTCCATC GTCTAACCAA 
GCAAGAAATC TTGAAAAATT TGAAAAGCCA GAAAAAGATA TTGAAGCCCA GTTGATACAT 
GAACCCTCAG TCAACGGCTC CTCAACTACA GTACCAAAGG CAGCATCCTC CGTCACTGCT 
GGAGTGGCCA GTTCACTCTC AGAGAAAATA GTCGATACCC TTGGGAACAG TCGGCCAGGG 
GCGCCGCTTA CCTCAGTCCA GATCCGCTTC ATTCAGAACA TGATACAAGA AACACTGGAT 
GACTTCAGGG AAGCTTGCCA TAGAGACATT GTGAATCTGC AAGTGGAAAT GATTAAACAG 
TTTCATATAC AGCTGAATGA AATGCACTCT TTGCTGGAAA GATACTCCGT GAATGAAGGC 
TTAGTGGCTG AGATCGAAAG GCTGCGAGAA GAAAACAAAA GGCTGCGGGC CCATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513397.1
CDS	1252..3351
Translation	MRGGTSGRPSLTVQAGAGSAARNGWSWASPVAPRLRGPVMQENLRFASSGDDVKIWDASFLTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVPLLELAEGQKQTCVDLNSTSMYLASGGLNNTVNIWDLKSKRLHRSLKDHKCEVTCVAYNWNDCYIASGSLSGEIILHSVTTNTSSTPFGHGSKQPIRHIKYSLFRKSLLGSVSDNGVVTLWDVNSQSSYHTFDSTHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTSVQCIAFQYSTSLTKASLSKGSSNKATAVNKRSVPVSSSSGAAQNSGIVREAPSPSIATVLPQPVTTALGKGSGAAQDEAGLARSKSTDIFSKETDAGKSQDFSSFDDTGKNSLGDMFSPIRDDAVVSKGGDESIGKGDGLDFLPQLNSVFPLRKNAGASSSLVLHSSPLNVLMGSSGKEENESHEPSAESKRAYLGKQEPKDAMKQFTKLISSGAEPGILNTCPSSNQARNLEKFEKPEKDIEAQLIHEPSVNGSSTTVPKAASSVTAGVASSLSEKIVDTLGNSRPGAPLTSVQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHIQLNEMHSLLERYSVNEGLVAEIERLREENKRLRAHF

Target ORF information:

RefSeq Version	XM_006513334.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513334.1

ORF Insert Sequence:

ATGCGAGGCG GGACTTCCGG CCGCCCAAGT TTAACAGTCC AGGCGGGAGC CGGAAGCGCA 
GCGAGGAACG GGTGGAGCTG GGCCTCCCCT GTGGCCCCAC GCTTGAGAGG CCCAGTCATG 
CAGGAAAACC TCAGATTTGC TTCATCAGGA GATGACGTTA AAATATGGGA TGCTTCGTTC 
CTGACATTGG TGGATAAATT CAACCCTCAC ACGTCACCGC ATGGAATCAG TTCAATCTGT 
TGGAGCAGCA ATAATAACTT TCTAGTGACA GCTTCTTCCA GTGGTGACAA AATAGTGGTG 
TCAAGTTGCA AATGTAAGCC CGTTCCACTT TTGGAGCTTG CGGAAGGGCA AAAGCAGACA 
TGTGTCGATT TAAATTCTAC ATCAATGTAT TTGGCAAGTG GAGGCCTAAA TAACACTGTT 
AATATTTGGG ATTTAAAGTC CAAACGACTT CATCGGTCTC TTAAGGATCA TAAATGTGAA 
GTAACTTGTG TGGCATACAA TTGGAATGAC TGTTACATTG CTTCTGGGTC TCTCAGTGGT 
GAGATTATCT TACACAGTGT AACCACTAAT ACATCCAGCA CACCTTTTGG CCATGGGAGT 
AAGCAGCCTA TCCGGCACAT CAAGTACTCA TTGTTTAGGA AGTCTCTCTT GGGCAGCGTT 
TCGGATAATG GAGTAGTGAC TCTCTGGGAT GTGAACAGTC AGAGTTCATA CCACACCTTT 
GACAGTACAC ACAAAGCCCC AGCTTCAGGC ATCTGCTTCT CTCCTGTCAA TGAACTGCTG 
TTTGTAACCA TAGGCCTGGA TAAAAGAATC ATCCTCTATG ATACTTCAAG TAAGAAGCTA 
GTGAAAACTT TAGTGGCCGA CACCCCCCTG ACTGCAGTAG ATTTTATGCC GGATGGAGCC 
ACTTTGGCTA TTGGATCTTC CCGTGGGAAA ATATATCAGT ATGACTTAAG GATGCTGAAA 
TCTCCCGTGA AAACCATCAG TGCCCACAAG ACATCTGTGC AGTGTATAGC ATTTCAGTAC 
TCCACATCTC TGACGAAGGC AAGTTTAAGC AAAGGCAGCT CAAATAAGGC CACAGCAGTG 
AACAAACGGA GTGTTCCTGT GAGCAGCAGC AGTGGGGCTG CCCAGAATTC TGGAATTGTC 
AGAGAAGCAC CCAGCCCTTC CATTGCCACA GTTCTCCCAC AGCCCGTGAC AACAGCTCTG 
GGCAAAGGAT CAGGGGCTGC TCAGGACGAA GCAGGTTTGG CTCGAAGCAA AAGTACAGAT 
ATTTTCTCTA AGGAAACAGA TGCTGGAAAA AGTCAGGATT TCTCCAGCTT TGATGATACT 
GGGAAAAATA GTTTAGGTGA CATGTTCTCA CCAATCAGAG ATGATGCTGT AGTCAGCAAA 
GGAGGTGATG AATCTATAGG CAAAGGAGAT GGCCTTGACT TTCTACCACA GCTAAACTCA 
GTGTTCCCTC TGAGGAAGAA TGCAGGTGCC TCCAGCAGTC TGGTGCTGCA CTCCAGCCCT 
CTTAATGTCC TCATGGGATC TTCAGGGAAA GAAGAGAATG AAAGCCATGA GCCGTCAGCT 
GAGTCTAAAA GAGCATACCT AGGGAAGCAG GAACCTAAGG ACGCCATGAA GCAGTTTACA 
AAGTTGATCT CCTCTGGTGC TGAACCTGGA ATTCTAAATA CCTGTCCATC GTCTAACCAA 
GCAAGAAATC TTGAAAAATT TGAAAAGCCA GAAAAAGATA TTGAAGCCCA GTTGATACAT 
GAACCCTCAG TCAACGGCTC CTCAACTACA GTACCAAAGG CAGCATCCTC CGTCACTGCT 
GGAGTGGCCA GTTCACTCTC AGAGAAAATA GTCGATACCC TTGGGAACAG TCGGCCAGGG 
GCGCCGCTTA CCTCAGTCCA GATCCGCTTC ATTCAGAACA TGATACAAGA AACACTGGAT 
GACTTCAGGG AAGCTTGCCA TAGAGACATT GTGAATCTGC AAGTGGAAAT GATTAAACAG 
TTTCATATAC AGCTGAATGA AATGCACTCT TTGCTGGAAA GATACTCCGT GAATGAAGGC 
TTAGTGGCTG AGATCGAAAG GCTGCGAGAA GAAAACAAAA GGCTGCGGGC CCATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd1 ( NM_008682.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032708.2 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), mRNA.

Price & Availability↑

CloneID	OMu02524
Clone ID Related Accession (Same CDS sequence)	NM_008682.2
Accession Version	NM_008682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	protein NEDD1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK153746.1 and BC052430.1. On Apr 1, 2008 this sequence version replaced NM_008682.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052430.1, AK153746.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGAAA ACCTCAGATT TGCTTCATCA GGAGATGACG TTAAAATATG GGATGCTTCG 
TTCCTGACAT TGGTGGATAA ATTCAACCCT CACACGTCAC CGCATGGAAT CAGTTCAATC 
TGTTGGAGCA GCAATAATAA CTTTCTAGTG ACAGCTTCTT CCAGTGGTGA CAAAATAGTG 
GTGTCAAGTT GCAAATGTAA GCCCGTTCCA CTTTTGGAGC TTGCGGAAGG GCAAAAGCAG 
ACATGTGTCG ATTTAAATTC TACATCAATG TATTTGGCAA GTGGAGGCCT AAATAACACT 
GTTAATATTT GGGATTTAAA GTCCAAACGA CTTCATCGGT CTCTTAAGGA TCATAAATGT 
GAAGTAACTT GTGTGGCATA CAATTGGAAT GACTGTTACA TTGCTTCTGG GTCTCTCAGT 
GGTGAGATTA TCTTACACAG TGTAACCACT AATACATCCA GCACACCTTT TGGCCATGGG 
AGTAAGCAGC CTATCCGGCA CATCAAGTAC TCATTGTTTA GGAAGTCTCT CTTGGGCAGC 
GTTTCGGATA ATGGAGTAGT GACTCTCTGG GATGTGAACA GTCAGAGTTC ATACCACACC 
TTTGACAGTA CACACAAAGC CCCAGCTTCA GGCATCTGCT TCTCTCCTGT CAATGAACTG 
CTGTTTGTAA CCATAGGCCT GGATAAAAGA ATCATCCTCT ATGATACTTC AAGTAAGAAG 
CTAGTGAAAA CTTTAGTGGC CGACACCCCC CTGACTGCAG TAGATTTTAT GCCGGATGGA 
GCCACTTTGG CTATTGGATC TTCCCGTGGG AAAATATATC AGTATGACTT AAGGATGCTG 
AAATCTCCCG TGAAAACCAT CAGTGCCCAC AAGACATCTG TGCAGTGTAT AGCATTTCAG 
TACTCCACAT CTCTGACGAA GGCAAGTTTA AGCAAAGGCA GCTCAAATAA GGCCACAGCA 
GTGAACAAAC GGAGTGTTCC TGTGAGCAGC AGCAGTGGGG CTGCCCAGAA TTCTGGAATT 
GTCAGAGAAG CACCCAGCCC TTCCATTGCC ACAGTTCTCC CACAGCCCGT GACAACAGCT 
CTGGGCAAAG GATCAGGGGC TGCTCAGGAC GAAGCAGGTT TGGCTCGAAG CAAAAGTACA 
GATATTTTCT CTAAGGAAAC AGATGCTGGA AAAAGTCAGG ATTTCTCCAG CTTTGATGAT 
ACTGGGAAAA ATAGTTTAGG TGACATGTTC TCACCAATCA GAGATGATGC TGTAGTCAGC 
AAAGGAGGTG ATGAATCTAT AGGCAAAGGA GATGGCCTTG ACTTTCTACC ACAGCTAAAC 
TCAGTGTTCC CTCTGAGGAA GAATGCAGGT GCCTCCAGCA GTCTGGTGCT GCACTCCAGC 
CCTCTTAATG TCCTCATGGG ATCTTCAGGG AAAGAAGAGA ATGAAAGCCA TGAGCCGTCA 
GCTGAGTCTA AAAGAGCATA CCTAGGGAAG CAGGAACCTA AGGACGCCAT GAAGCAGTTT 
ACAAAGTTGA TCTCCTCTGG TGCTGAACCT GGAATTCTAA ATACCTGTCC ATCGTCTAAC 
CAAGCAAGAA ATCTTGAAAA ATTTGAAAAG CCAGAAAAAG ATATTGAAGC CCAGTTGATA 
CATGAACCCT CAGTCAACGG CTCCTCAACT ACAGTACCAA AGGCAGCATC CTCCGTCACT 
GCTGGAGTGG CCAGTTCACT CTCAGAGAAA ATAGTCGATA CCCTTGGGAA CAGTCGGCCA 
GGGGCGCCGC TTACCTCAGT CCAGATCCGC TTCATTCAGA ACATGATACA AGAAACACTG 
GATGACTTCA GGGAAGCTTG CCATAGAGAC ATTGTGAATC TGCAAGTGGA AATGATTAAA 
CAGTTTCATA TACAGCTGAA TGAAATGCAC TCTTTGCTGG AAAGATACTC CGTGAATGAA 
GGCTTAGTGG CTGAGATCGA AAGGCTGCGA GAAGAAAACA AAAGGCTGCG GGCCCATTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032708.2
CDS	105..2087
Translation	MQENLRFASSGDDVKIWDASFLTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVPLLELAEGQKQTCVDLNSTSMYLASGGLNNTVNIWDLKSKRLHRSLKDHKCEVTCVAYNWNDCYIASGSLSGEIILHSVTTNTSSTPFGHGSKQPIRHIKYSLFRKSLLGSVSDNGVVTLWDVNSQSSYHTFDSTHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTSVQCIAFQYSTSLTKASLSKGSSNKATAVNKRSVPVSSSSGAAQNSGIVREAPSPSIATVLPQPVTTALGKGSGAAQDEAGLARSKSTDIFSKETDAGKSQDFSSFDDTGKNSLGDMFSPIRDDAVVSKGGDESIGKGDGLDFLPQLNSVFPLRKNAGASSSLVLHSSPLNVLMGSSGKEENESHEPSAESKRAYLGKQEPKDAMKQFTKLISSGAEPGILNTCPSSNQARNLEKFEKPEKDIEAQLIHEPSVNGSSTTVPKAASSVTAGVASSLSEKIVDTLGNSRPGAPLTSVQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHIQLNEMHSLLERYSVNEGLVAEIERLREENKRLRAHF

Target ORF information:

RefSeq Version	NM_008682.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008682.2

ORF Insert Sequence:

ATGCAGGAAA ACCTCAGATT TGCTTCATCA GGAGATGACG TTAAAATATG GGATGCTTCG 
TTCCTGACAT TGGTGGATAA ATTCAACCCT CACACGTCAC CGCATGGAAT CAGTTCAATC 
TGTTGGAGCA GCAATAATAA CTTTCTAGTG ACAGCTTCTT CCAGTGGTGA CAAAATAGTG 
GTGTCAAGTT GCAAATGTAA GCCCGTTCCA CTTTTGGAGC TTGCGGAAGG GCAAAAGCAG 
ACATGTGTCG ATTTAAATTC TACATCAATG TATTTGGCAA GTGGAGGCCT AAATAACACT 
GTTAATATTT GGGATTTAAA GTCCAAACGA CTTCATCGGT CTCTTAAGGA TCATAAATGT 
GAAGTAACTT GTGTGGCATA CAATTGGAAT GACTGTTACA TTGCTTCTGG GTCTCTCAGT 
GGTGAGATTA TCTTACACAG TGTAACCACT AATACATCCA GCACACCTTT TGGCCATGGG 
AGTAAGCAGC CTATCCGGCA CATCAAGTAC TCATTGTTTA GGAAGTCTCT CTTGGGCAGC 
GTTTCGGATA ATGGAGTAGT GACTCTCTGG GATGTGAACA GTCAGAGTTC ATACCACACC 
TTTGACAGTA CACACAAAGC CCCAGCTTCA GGCATCTGCT TCTCTCCTGT CAATGAACTG 
CTGTTTGTAA CCATAGGCCT GGATAAAAGA ATCATCCTCT ATGATACTTC AAGTAAGAAG 
CTAGTGAAAA CTTTAGTGGC CGACACCCCC CTGACTGCAG TAGATTTTAT GCCGGATGGA 
GCCACTTTGG CTATTGGATC TTCCCGTGGG AAAATATATC AGTATGACTT AAGGATGCTG 
AAATCTCCCG TGAAAACCAT CAGTGCCCAC AAGACATCTG TGCAGTGTAT AGCATTTCAG 
TACTCCACAT CTCTGACGAA GGCAAGTTTA AGCAAAGGCA GCTCAAATAA GGCCACAGCA 
GTGAACAAAC GGAGTGTTCC TGTGAGCAGC AGCAGTGGGG CTGCCCAGAA TTCTGGAATT 
GTCAGAGAAG CACCCAGCCC TTCCATTGCC ACAGTTCTCC CACAGCCCGT GACAACAGCT 
CTGGGCAAAG GATCAGGGGC TGCTCAGGAC GAAGCAGGTT TGGCTCGAAG CAAAAGTACA 
GATATTTTCT CTAAGGAAAC AGATGCTGGA AAAAGTCAGG ATTTCTCCAG CTTTGATGAT 
ACTGGGAAAA ATAGTTTAGG TGACATGTTC TCACCAATCA GAGATGATGC TGTAGTCAGC 
AAAGGAGGTG ATGAATCTAT AGGCAAAGGA GATGGCCTTG ACTTTCTACC ACAGCTAAAC 
TCAGTGTTCC CTCTGAGGAA GAATGCAGGT GCCTCCAGCA GTCTGGTGCT GCACTCCAGC 
CCTCTTAATG TCCTCATGGG ATCTTCAGGG AAAGAAGAGA ATGAAAGCCA TGAGCCGTCA 
GCTGAGTCTA AAAGAGCATA CCTAGGGAAG CAGGAACCTA AGGACGCCAT GAAGCAGTTT 
ACAAAGTTGA TCTCCTCTGG TGCTGAACCT GGAATTCTAA ATACCTGTCC ATCGTCTAAC 
CAAGCAAGAA ATCTTGAAAA ATTTGAAAAG CCAGAAAAAG ATATTGAAGC CCAGTTGATA 
CATGAACCCT CAGTCAACGG CTCCTCAACT ACAGTACCAA AGGCAGCATC CTCCGTCACT 
GCTGGAGTGG CCAGTTCACT CTCAGAGAAA ATAGTCGATA CCCTTGGGAA CAGTCGGCCA 
GGGGCGCCGC TTACCTCAGT CCAGATCCGC TTCATTCAGA ACATGATACA AGAAACACTG 
GATGACTTCA GGGAAGCTTG CCATAGAGAC ATTGTGAATC TGCAAGTGGA AATGATTAAA 
CAGTTTCATA TACAGCTGAA TGAAATGCAC TCTTTGCTGG AAAGATACTC CGTGAATGAA 
GGCTTAGTGG CTGAGATCGA AAGGCTGCGA GAAGAAAACA AAAGGCTGCG GGCCCATTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Divergent regulation of functionally distinct ?-tubulin complexes during differentiation.
The Journal of cell biology213(6)679-92(2016 Jun)
Muroyama A,Seldin L,Lechler T

CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination.
Nature communications67676(2015 Jul)
Marjanovi? M,S?nchez-Huertas C,Terr? B,G?mez R,Scheel JF,Pacheco S,Knobel PA,Mart?nez-Marchal A,Aivio S,Palenzuela L,Wolfrum U,McKinnon PJ,Suja JA,Roig I,Costanzo V,L?ders J,Stracker TH

Intraperitoneal delivery of a small interfering RNA targeting NEDD1 prolongs the survival of scirrhous gastric cancer model mice.
Cancer science104(2)214-22(2013 Feb)
Fujita T,Yanagihara K,Takeshita F,Aoyagi K,Nishimura T,Takigahira M,Chiwaki F,Fukagawa T,Katai H,Ochiya T,Sakamoto H,Konno H,Yoshida T,Sasaki H

Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip.
Gene expression patterns : GEP11(5-6)349-59(2011 Jun-Jul)
Machold R,Klein C,Fishell G

A potential role for NEDD1 and the centrosome in senescence of mouse embryonic fibroblasts.
Cell death & disease1e35(2010 Apr)
Manning JA,Kumar S

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Nedd1 cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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