Mstn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mstn
Entrez Gene ID	17700
Full Name	myostatin
Synonyms	Cmpt,Gdf8
General protein information	Preferred Names myostatin Names growth/differentiation factor 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C1.1\|1 26.99 cM
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Homozygous knockout mice for this gene exhibit increased muscle mass and bone density, and reduced adiposity. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010834.3	NP_034964.1	growth/differentiation factor 8 preproprotein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MSTN	NP_001001461.1
Xenopus tropicalis (tropical clawed frog)	LOC100496408	XP_002931568.1
Homo sapiens (human)	MSTN	NP_005250.1
	MSTN	NP_001073588.1
Pan troglodytes (chimpanzee)	MSTN	NP_001073388.1
Canis lupus familiaris (dog)	MSTN	NP_001002959.1
Bos taurus (cattle)	MSTN	NP_001001525.1
Mus musculus (house mouse)	Mstn	NP_034964.1
Danio rerio (zebrafish)	mstnb	NP_571094.1
Rattus norvegicus (Norway rat)	Mstn	NP_062024.1
Drosophila melanogaster (fruit fly)	myo	NP_726604.1

Gene Ontology

The following Mstn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mstn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19703	NM_010834.3 Latest version!	Mus musculus myostatin (Mstn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mstn ( NM_010834.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034964.1 Mus musculus myostatin (Mstn), mRNA.

Price & Availability↑

CloneID	OMu19703
Clone ID Related Accession (Same CDS sequence)	NM_010834.3
Accession Version	NM_010834.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	growth/differentiation factor 8 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK142222.1 and AK132415.1. On Aug 27, 2015 this sequence version replaced NM_010834.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK142222.1, AK132415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGATGCAAA AACTGCAAAT GTATGTTTAT ATTTACCTGT TCATGCTGAT TGCTGCTGGC 
CCAGTGGATC TAAATGAGGG CAGTGAGAGA GAAGAAAATG TGGAAAAAGA GGGGCTGTGT 
AATGCATGTG CGTGGAGACA AAACACGAGG TACTCCAGAA TAGAAGCCAT AAAAATTCAA 
ATCCTCAGTA AGCTGCGCCT GGAAACAGCT CCTAACATCA GCAAAGATGC TATAAGACAA 
CTTCTGCCAA GAGCGCCTCC ACTCCGGGAA CTGATCGATC AGTACGACGT CCAGAGGGAT 
GACAGCAGTG ATGGCTCTTT GGAAGATGAC GATTATCACG CTACCACGGA AACAATCATT 
ACCATGCCTA CAGAGTCTGA CTTTCTAATG CAAGCGGATG GCAAGCCCAA ATGTTGCTTT 
TTTAAATTTA GCTCTAAAAT ACAGTACAAC AAAGTAGTAA AAGCCCAACT GTGGATATAT 
CTCAGACCCG TCAAGACTCC TACAACAGTG TTTGTGCAAA TCCTGAGACT CATCAAACCC 
ATGAAAGACG GTACAAGGTA TACTGGAATC CGATCTCTGA AACTTGACAT GAGCCCAGGC 
ACTGGTATTT GGCAGAGTAT TGATGTGAAG ACAGTGTTGC AAAATTGGCT CAAACAGCCT 
GAATCCAACT TAGGCATTGA AATCAAAGCT TTGGATGAGA ATGGCCATGA TCTTGCTGTA 
ACCTTCCCAG GACCAGGAGA AGATGGGCTG AATCCCTTTT TAGAAGTCAA GGTGACAGAC 
ACACCCAAGA GGTCCCGGAG AGACTTTGGG CTTGACTGCG ATGAGCACTC CACGGAATCC 
CGGTGCTGCC GCTACCCCCT CACGGTCGAT TTTGAAGCCT TTGGATGGGA CTGGATTATC 
GCACCCAAAA GATATAAGGC CAATTACTGC TCAGGAGAGT GTGAATTTGT GTTTTTACAA 
AAATATCCGC ATACTCATCT TGTGCACCAA GCAAACCCCA GAGGCTCAGC AGGCCCTTGC 
TGCACTCCGA CAAAAATGTC TCCCATTAAT ATGCTATATT TTAATGGCAA AGAACAAATA 
ATATATGGGA AAATTCCAGC CATGGTAGTA GACCGCTGTG GGTGCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034964.1
CDS	127..1257
Translation	MMQKLQMYVYIYLFMLIAAGPVDLNEGSEREENVEKEGLCNACAWRQNTRYSRIEAIKIQILSKLRLETAPNISKDAIRQLLPRAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQADGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMSPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS

Target ORF information:

RefSeq Version	NM_010834.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myostatin (Mstn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010834.3

ORF Insert Sequence:

ATGATGCAAA AACTGCAAAT GTATGTTTAT ATTTACCTGT TCATGCTGAT TGCTGCTGGC 
CCAGTGGATC TAAATGAGGG CAGTGAGAGA GAAGAAAATG TGGAAAAAGA GGGGCTGTGT 
AATGCATGTG CGTGGAGACA AAACACGAGG TACTCCAGAA TAGAAGCCAT AAAAATTCAA 
ATCCTCAGTA AGCTGCGCCT GGAAACAGCT CCTAACATCA GCAAAGATGC TATAAGACAA 
CTTCTGCCAA GAGCGCCTCC ACTCCGGGAA CTGATCGATC AGTACGACGT CCAGAGGGAT 
GACAGCAGTG ATGGCTCTTT GGAAGATGAC GATTATCACG CTACCACGGA AACAATCATT 
ACCATGCCTA CAGAGTCTGA CTTTCTAATG CAAGCGGATG GCAAGCCCAA ATGTTGCTTT 
TTTAAATTTA GCTCTAAAAT ACAGTACAAC AAAGTAGTAA AAGCCCAACT GTGGATATAT 
CTCAGACCCG TCAAGACTCC TACAACAGTG TTTGTGCAAA TCCTGAGACT CATCAAACCC 
ATGAAAGACG GTACAAGGTA TACTGGAATC CGATCTCTGA AACTTGACAT GAGCCCAGGC 
ACTGGTATTT GGCAGAGTAT TGATGTGAAG ACAGTGTTGC AAAATTGGCT CAAACAGCCT 
GAATCCAACT TAGGCATTGA AATCAAAGCT TTGGATGAGA ATGGCCATGA TCTTGCTGTA 
ACCTTCCCAG GACCAGGAGA AGATGGGCTG AATCCCTTTT TAGAAGTCAA GGTGACAGAC 
ACACCCAAGA GGTCCCGGAG AGACTTTGGG CTTGACTGCG ATGAGCACTC CACGGAATCC 
CGGTGCTGCC GCTACCCCCT CACGGTCGAT TTTGAAGCCT TTGGATGGGA CTGGATTATC 
GCACCCAAAA GATATAAGGC CAATTACTGC TCAGGAGAGT GTGAATTTGT GTTTTTACAA 
AAATATCCGC ATACTCATCT TGTGCACCAA GCAAACCCCA GAGGCTCAGC AGGCCCTTGC 
TGCACTCCGA CAAAAATGTC TCCCATTAAT ATGCTATATT TTAATGGCAA AGAACAAATA 
ATATATGGGA AAATTCCAGC CATGGTAGTA GACCGCTGTG GGTGCTCATG A