ORF » Species Summary » Mus musculus » Mid1 cDNA ORF clone

Mid1 cDNA ORF clone, Mus musculus(house mouse)

The following Mid1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu13871 NM_001290506.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu13877 NM_001290512.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu56596 XM_006528736.4
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu56597 XM_006528737.4
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu56599 XM_006528739.4
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu13850 NM_001290505.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu13850 NM_001290504.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu13885 NM_010797.3
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu13850 XM_011247789.3
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu13850 XM_030251241.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu13871 XM_030251244.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu13885 XM_017318408.2
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu13885 XM_017318410.2
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu13885 XM_017318409.2
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu83087 XM_017318411.2
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu83088 XM_017318412.2
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu83089 XM_017318413.2
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu90363 XM_030251243.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu90363 XM_030251242.1
Latest version!		 Mus musculus midline 1 (Mid1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu56599 XM_006528739.3 Mus musculus midline 1 (Mid1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu13885 XM_017318410.1 Mus musculus midline 1 (Mid1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu13850 XM_011247789.2 Mus musculus midline 1 (Mid1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu56596 XM_006528736.3 Mus musculus midline 1 (Mid1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu13885 XM_017318408.1 Mus musculus midline 1 (Mid1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu83087 XM_017318411.1 Mus musculus midline 1 (Mid1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu83088 XM_017318412.1 Mus musculus midline 1 (Mid1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu56597 XM_006528737.3 Mus musculus midline 1 (Mid1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OMu13885 XM_017318409.1 Mus musculus midline 1 (Mid1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu83089 XM_017318413.1 Mus musculus midline 1 (Mid1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Mid1 ( XM_006528737.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528800.1 Mus musculus midline 1 (Mid1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OMu56597
    Clone ID Related Accession (Same CDS sequence) XM_006528737.4 , XM_006528737.3
    Accession Version XM_006528737.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528800.1
    CDS263..1465
    Translation

    Target ORF information:

    RefSeq Version XM_006528737.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528737.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( NM_001290506.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277435.1 Mus musculus midline 1 (Mid1), transcript variant 5, mRNA.
    Price & Availability↑
    CloneID OMu13871
    Clone ID Related Accession (Same CDS sequence) NM_001290506.1 , XM_030251244.1
    Accession Version NM_001290506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-02
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672015.7 and CR478112.2. On Mar 13, 2014 this sequence version replaced XM_006528734.1. Transcript Variant: This variant (5) differs in the 5' UTR and uses two alternate in-frame splice sites, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AGTCTGTGTA ACTCGGCGGA CAGCTGGATG 
    ATCGTGCCCA ACATCAAGCA GAACCACTAC ACCGTGCACG GCCTGCAAAG TGGCACCAAG 
    TATATCTTCA CGGTGAAGGC CATCAACCAG GCGGGCAGCC GTAGCAGCGA GCCCGGAAAG 
    CTGAAGACCA ACAGTCAGCC GTTTAGACTG GATCCCAAAT CGGCTCATCG CAAGCTGAAG 
    GTGTCCCACG ACAACCTGAC TGTCGAGCGC GACGAGTCGT CCTCTAAGAA GAGTCACGCG 
    CCGGAGCGCT TCGCTGGTCA GGGGAGCTAC GGAGTGGCTG GCAACGTGTT CATCGACAGC 
    GGCCGTCACT ACTGGGAAGT GGTCACCAGC GGAAGCACAT GGTACGCCAT CGGCCTGGCG 
    TACAGATCGG CGCCGAAACA CGAGTGGATC GGGAAGAACG CGGCGTCCTG GGCCCTCTGC 
    CGCTGCCACA ACCACTGGGC GGTGCGACAC GACGGCAAGG AGACCCCCAT CGCGCCGGCC 
    CCTCACCTCA GGCGCGTCGG CGTCCTGCTG GACTACGACA ACGGCTCCAT CGCCTTCTAC 
    GACGCTCTGA GCTCCGTCCA CCTCCACACC TTCCACGCGG CGCTCGCGCA GCCCGTGTGC 
    CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACCATCGTCA CGGGTCTGCC CATCCCGGAC 
    CATCTGGACT GTACGGAGCA GCGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277435.1
    CDS348..2237
    Translation

    Target ORF information:

    RefSeq Version NM_001290506.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AGTCTGTGTA ACTCGGCGGA CAGCTGGATG 
    ATCGTGCCCA ACATCAAGCA GAACCACTAC ACCGTGCACG GCCTGCAAAG TGGCACCAAG 
    TATATCTTCA CGGTGAAGGC CATCAACCAG GCGGGCAGCC GTAGCAGCGA GCCCGGAAAG 
    CTGAAGACCA ACAGTCAGCC GTTTAGACTG GATCCCAAAT CGGCTCATCG CAAGCTGAAG 
    GTGTCCCACG ACAACCTGAC TGTCGAGCGC GACGAGTCGT CCTCTAAGAA GAGTCACGCG 
    CCGGAGCGCT TCGCTGGTCA GGGGAGCTAC GGAGTGGCTG GCAACGTGTT CATCGACAGC 
    GGCCGTCACT ACTGGGAAGT GGTCACCAGC GGAAGCACAT GGTACGCCAT CGGCCTGGCG 
    TACAGATCGG CGCCGAAACA CGAGTGGATC GGGAAGAACG CGGCGTCCTG GGCCCTCTGC 
    CGCTGCCACA ACCACTGGGC GGTGCGACAC GACGGCAAGG AGACCCCCAT CGCGCCGGCC 
    CCTCACCTCA GGCGCGTCGG CGTCCTGCTG GACTACGACA ACGGCTCCAT CGCCTTCTAC 
    GACGCTCTGA GCTCCGTCCA CCTCCACACC TTCCACGCGG CGCTCGCGCA GCCCGTGTGC 
    CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACCATCGTCA CGGGTCTGCC CATCCCGGAC 
    CATCTGGACT GTACGGAGCA GCGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( NM_001290512.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277441.1 Mus musculus midline 1 (Mid1), transcript variant 6, mRNA.
    Price & Availability↑
    CloneID OMu13877
    Clone ID Related Accession (Same CDS sequence) NM_001290512.1
    Accession Version NM_001290512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-02
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671865.11, AL672015.7 and CR478112.2. Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an alternate in-frame splice site, compared to variant 1. It initiates translation at an alternate start codon. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160120.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGACCTTGC ACAAGGATGC ATTTTCAAAG CTTGACACTG AGCAAAACTT GGAGAGTAAT 
    CTCACCAATC TTATTAAGAG AAACACAGAA CTGGAGACTC TTTTGGCTAA ACTCATCCAA 
    ACTTGTCAAC ATGTTGAAGT CAATGCATCC CGTCAAGAAG CCAAACTGAC AGAAGAATGT 
    GATCTTCTCA TTGAAATCAT TCAGCAACGA AGACAAATTA TTGGAACAAA GATTAAAGAA 
    GGCAAGGTGA TCAGGCTCCG CAAGTTAGCT CAGCAGATTG CAAACTGTAA ACAGTGCCTT 
    GAGAGGTCTG CATCGCTCAT CTCGCAAGCG GAGCACTCGC TGAAGGAAAA TGACCACGCC 
    CGTTTTCTAC AGACAGCAAA GAATATCACT GAGAGAGTCT CCATGGCAAC TGCATCCTCC 
    CAGGTCCTAA TTCCCGAAAT CAACCTCAAT GACACGTTTG ACACTTTTGC CTTGGATTTT 
    TCCCGGGAGA AGAAACTGCT AGAATGTCTG GATTACCTAA CAGCTCCCAA CCCTCCCGCG 
    ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC GACACCATCA CCGTCCACTG GACCTCAGAG 
    GACGAGTTCA GCGTGGTCTC CTACGAGCTC CAGTACACCA TATTCACCGG ACAAGCCAAT 
    GTTGTCAGTC TGTGTAACTC GGCGGACAGC TGGATGATCG TGCCCAACAT CAAGCAGAAC 
    CACTACACCG TGCACGGCCT GCAAAGTGGC ACCAAGTATA TCTTCACGGT GAAGGCCATC 
    AACCAGGCGG GCAGCCGTAG CAGCGAGCCC GGAAAGCTGA AGACCAACAG TCAGCCGTTT 
    AGACTGGATC CCAAATCGGC TCATCGCAAG CTGAAGGTGT CCCACGACAA CCTGACTGTC 
    GAGCGCGACG AGTCGTCCTC TAAGAAGAGT CACGCGCCGG AGCGCTTCGC TGGTCAGGGG 
    AGCTACGGAG TGGCTGGCAA CGTGTTCATC GACAGCGGCC GTCACTACTG GGAAGTGGTC 
    ACCAGCGGAA GCACATGGTA CGCCATCGGC CTGGCGTACA GATCGGCGCC GAAACACGAG 
    TGGATCGGGA AGAACGCGGC GTCCTGGGCC CTCTGCCGCT GCCACAACCA CTGGGCGGTG 
    CGACACGACG GCAAGGAGAC CCCCATCGCG CCGGCCCCTC ACCTCAGGCG CGTCGGCGTC 
    CTGCTGGACT ACGACAACGG CTCCATCGCC TTCTACGACG CTCTGAGCTC CGTCCACCTC 
    CACACCTTCC ACGCGGCGCT CGCGCAGCCC GTGTGCCCCA CCTTCACCGT GTGGAACAAG 
    TGTCTGACCA TCGTCACGGG TCTGCCCATC CCGGACCATC TGGACTGTAC GGAGCAGCGA 
    CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277441.1
    CDS186..1571
    Translation

    Target ORF information:

    RefSeq Version NM_001290512.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGACCTTGC ACAAGGATGC ATTTTCAAAG CTTGACACTG AGCAAAACTT GGAGAGTAAT 
    CTCACCAATC TTATTAAGAG AAACACAGAA CTGGAGACTC TTTTGGCTAA ACTCATCCAA 
    ACTTGTCAAC ATGTTGAAGT CAATGCATCC CGTCAAGAAG CCAAACTGAC AGAAGAATGT 
    GATCTTCTCA TTGAAATCAT TCAGCAACGA AGACAAATTA TTGGAACAAA GATTAAAGAA 
    GGCAAGGTGA TCAGGCTCCG CAAGTTAGCT CAGCAGATTG CAAACTGTAA ACAGTGCCTT 
    GAGAGGTCTG CATCGCTCAT CTCGCAAGCG GAGCACTCGC TGAAGGAAAA TGACCACGCC 
    CGTTTTCTAC AGACAGCAAA GAATATCACT GAGAGAGTCT CCATGGCAAC TGCATCCTCC 
    CAGGTCCTAA TTCCCGAAAT CAACCTCAAT GACACGTTTG ACACTTTTGC CTTGGATTTT 
    TCCCGGGAGA AGAAACTGCT AGAATGTCTG GATTACCTAA CAGCTCCCAA CCCTCCCGCG 
    ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC GACACCATCA CCGTCCACTG GACCTCAGAG 
    GACGAGTTCA GCGTGGTCTC CTACGAGCTC CAGTACACCA TATTCACCGG ACAAGCCAAT 
    GTTGTCAGTC TGTGTAACTC GGCGGACAGC TGGATGATCG TGCCCAACAT CAAGCAGAAC 
    CACTACACCG TGCACGGCCT GCAAAGTGGC ACCAAGTATA TCTTCACGGT GAAGGCCATC 
    AACCAGGCGG GCAGCCGTAG CAGCGAGCCC GGAAAGCTGA AGACCAACAG TCAGCCGTTT 
    AGACTGGATC CCAAATCGGC TCATCGCAAG CTGAAGGTGT CCCACGACAA CCTGACTGTC 
    GAGCGCGACG AGTCGTCCTC TAAGAAGAGT CACGCGCCGG AGCGCTTCGC TGGTCAGGGG 
    AGCTACGGAG TGGCTGGCAA CGTGTTCATC GACAGCGGCC GTCACTACTG GGAAGTGGTC 
    ACCAGCGGAA GCACATGGTA CGCCATCGGC CTGGCGTACA GATCGGCGCC GAAACACGAG 
    TGGATCGGGA AGAACGCGGC GTCCTGGGCC CTCTGCCGCT GCCACAACCA CTGGGCGGTG 
    CGACACGACG GCAAGGAGAC CCCCATCGCG CCGGCCCCTC ACCTCAGGCG CGTCGGCGTC 
    CTGCTGGACT ACGACAACGG CTCCATCGCC TTCTACGACG CTCTGAGCTC CGTCCACCTC 
    CACACCTTCC ACGCGGCGCT CGCGCAGCCC GTGTGCCCCA CCTTCACCGT GTGGAACAAG 
    TGTCTGACCA TCGTCACGGG TCTGCCCATC CCGGACCATC TGGACTGTAC GGAGCAGCGA 
    CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_006528736.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528799.1 Mus musculus midline 1 (Mid1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OMu56596
    Clone ID Related Accession (Same CDS sequence) XM_006528736.3 , XM_006528736.4
    Accession Version XM_006528736.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528736.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAACGTGG CCTGTGACGG CACCTGTCTC CTCGGCTCCG CAGGTCTGTG TAACTCGGCG 
    GACAGCTGGA TGATCGTGCC CAACATCAAG CAGAACCACT ACACCGTGCA CGGCCTGCAA 
    AGTGGCACCA AGTATATCTT CACGGTGAAG GCCATCAACC AGGCGGGCAG CCGTAGCAGC 
    GAGCCCGGAA AGCTGAAGAC CAACAGTCAG CCGTTTAGAC TGGATCCCAA ATCGGCTCAT 
    CGCAAGCTGA AGGTGTCCCA CGACAACCTG ACTGTCGAGC GCGACGAGTC GTCCTCTAAG 
    AAGAGTCACG CGCCGGAGCG CTTCGCTGGT CAGGGGAGCT ACGGAGTGGC TGGCAACGTG 
    TTCATCGACA GCGGCCGTCA CTACTGGGAA GTGGTCACCA GCGGAAGCAC ATGGTACGCC 
    ATCGGCCTGG CGTACAGATC GGCGCCGAAA CACGAGTGGA TCGGGAAGAA CGCGGCGTCC 
    TGGGCCCTCT GCCGCTGCCA CAACCACTGG GCGGTGCGAC ACGACGGCAA GGAGACCCCC 
    ATCGCGCCGG CCCCTCACCT CAGGCGCGTC GGCGTCCTGC TGGACTACGA CAACGGCTCC 
    ATCGCCTTCT ACGACGCTCT GAGCTCCGTC CACCTCCACA CCTTCCACGC GGCGCTCGCG 
    CAGCCCGTGT GCCCCACCTT CACCGTGTGG AACAAGTGTC TGACCATCGT CACGGGTCTG 
    CCCATCCCGG ACCATCTGGA CTGTACGGAG CAGCGACCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528799.1
    CDS268..1509
    Translation

    Target ORF information:

    RefSeq Version XM_006528736.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528736.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAACGTGG CCTGTGACGG CACCTGTCTC CTCGGCTCCG CAGGTCTGTG TAACTCGGCG 
    GACAGCTGGA TGATCGTGCC CAACATCAAG CAGAACCACT ACACCGTGCA CGGCCTGCAA 
    AGTGGCACCA AGTATATCTT CACGGTGAAG GCCATCAACC AGGCGGGCAG CCGTAGCAGC 
    GAGCCCGGAA AGCTGAAGAC CAACAGTCAG CCGTTTAGAC TGGATCCCAA ATCGGCTCAT 
    CGCAAGCTGA AGGTGTCCCA CGACAACCTG ACTGTCGAGC GCGACGAGTC GTCCTCTAAG 
    AAGAGTCACG CGCCGGAGCG CTTCGCTGGT CAGGGGAGCT ACGGAGTGGC TGGCAACGTG 
    TTCATCGACA GCGGCCGTCA CTACTGGGAA GTGGTCACCA GCGGAAGCAC ATGGTACGCC 
    ATCGGCCTGG CGTACAGATC GGCGCCGAAA CACGAGTGGA TCGGGAAGAA CGCGGCGTCC 
    TGGGCCCTCT GCCGCTGCCA CAACCACTGG GCGGTGCGAC ACGACGGCAA GGAGACCCCC 
    ATCGCGCCGG CCCCTCACCT CAGGCGCGTC GGCGTCCTGC TGGACTACGA CAACGGCTCC 
    ATCGCCTTCT ACGACGCTCT GAGCTCCGTC CACCTCCACA CCTTCCACGC GGCGCTCGCG 
    CAGCCCGTGT GCCCCACCTT CACCGTGTGG AACAAGTGTC TGACCATCGT CACGGGTCTG 
    CCCATCCCGG ACCATCTGGA CTGTACGGAG CAGCGACCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_006528739.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528802.1 Mus musculus midline 1 (Mid1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OMu56599
    Clone ID Related Accession (Same CDS sequence) XM_006528739.3 , XM_006528739.4
    Accession Version XM_006528739.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528739.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528802.1
    CDS243..1265
    Translation

    Target ORF information:

    RefSeq Version XM_006528739.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528739.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_006528736.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528799.1 Mus musculus midline 1 (Mid1), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OMu56596
    Clone ID Related Accession (Same CDS sequence) XM_006528736.3 , XM_006528736.4
    Accession Version XM_006528736.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528736.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAACGTGG CCTGTGACGG CACCTGTCTC CTCGGCTCCG CAGGTCTGTG TAACTCGGCG 
    GACAGCTGGA TGATCGTGCC CAACATCAAG CAGAACCACT ACACCGTGCA CGGCCTGCAA 
    AGTGGCACCA AGTATATCTT CACGGTGAAG GCCATCAACC AGGCGGGCAG CCGTAGCAGC 
    GAGCCCGGAA AGCTGAAGAC CAACAGTCAG CCGTTTAGAC TGGATCCCAA ATCGGCTCAT 
    CGCAAGCTGA AGGTGTCCCA CGACAACCTG ACTGTCGAGC GCGACGAGTC GTCCTCTAAG 
    AAGAGTCACG CGCCGGAGCG CTTCGCTGGT CAGGGGAGCT ACGGAGTGGC TGGCAACGTG 
    TTCATCGACA GCGGCCGTCA CTACTGGGAA GTGGTCACCA GCGGAAGCAC ATGGTACGCC 
    ATCGGCCTGG CGTACAGATC GGCGCCGAAA CACGAGTGGA TCGGGAAGAA CGCGGCGTCC 
    TGGGCCCTCT GCCGCTGCCA CAACCACTGG GCGGTGCGAC ACGACGGCAA GGAGACCCCC 
    ATCGCGCCGG CCCCTCACCT CAGGCGCGTC GGCGTCCTGC TGGACTACGA CAACGGCTCC 
    ATCGCCTTCT ACGACGCTCT GAGCTCCGTC CACCTCCACA CCTTCCACGC GGCGCTCGCG 
    CAGCCCGTGT GCCCCACCTT CACCGTGTGG AACAAGTGTC TGACCATCGT CACGGGTCTG 
    CCCATCCCGG ACCATCTGGA CTGTACGGAG CAGCGACCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528799.1
    CDS148..1389
    Translation

    Target ORF information:

    RefSeq Version XM_006528736.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528736.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAACGTGG CCTGTGACGG CACCTGTCTC CTCGGCTCCG CAGGTCTGTG TAACTCGGCG 
    GACAGCTGGA TGATCGTGCC CAACATCAAG CAGAACCACT ACACCGTGCA CGGCCTGCAA 
    AGTGGCACCA AGTATATCTT CACGGTGAAG GCCATCAACC AGGCGGGCAG CCGTAGCAGC 
    GAGCCCGGAA AGCTGAAGAC CAACAGTCAG CCGTTTAGAC TGGATCCCAA ATCGGCTCAT 
    CGCAAGCTGA AGGTGTCCCA CGACAACCTG ACTGTCGAGC GCGACGAGTC GTCCTCTAAG 
    AAGAGTCACG CGCCGGAGCG CTTCGCTGGT CAGGGGAGCT ACGGAGTGGC TGGCAACGTG 
    TTCATCGACA GCGGCCGTCA CTACTGGGAA GTGGTCACCA GCGGAAGCAC ATGGTACGCC 
    ATCGGCCTGG CGTACAGATC GGCGCCGAAA CACGAGTGGA TCGGGAAGAA CGCGGCGTCC 
    TGGGCCCTCT GCCGCTGCCA CAACCACTGG GCGGTGCGAC ACGACGGCAA GGAGACCCCC 
    ATCGCGCCGG CCCCTCACCT CAGGCGCGTC GGCGTCCTGC TGGACTACGA CAACGGCTCC 
    ATCGCCTTCT ACGACGCTCT GAGCTCCGTC CACCTCCACA CCTTCCACGC GGCGCTCGCG 
    CAGCCCGTGT GCCCCACCTT CACCGTGTGG AACAAGTGTC TGACCATCGT CACGGGTCTG 
    CCCATCCCGG ACCATCTGGA CTGTACGGAG CAGCGACCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_006528737.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528800.1 Mus musculus midline 1 (Mid1), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID OMu56597
    Clone ID Related Accession (Same CDS sequence) XM_006528737.4 , XM_006528737.3
    Accession Version XM_006528737.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528737.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528800.1
    CDS148..1350
    Translation

    Target ORF information:

    RefSeq Version XM_006528737.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528737.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTC TTCTGCTTCC GTGGGGACTG AGTTTTGGCC TCTGCCTGGG GAGTTCCATC 
    GAGGTGATCA GGCTCCGCAA GTTAGCTCAG CAGATTGCAA ACTGTAAACA GTGCCTTGAG 
    AGGTCTGCAT CGCTCATCTC GCAAGCGGAG CACTCGCTGA AGGAAAATGA CCACGCCCGT 
    TTTCTACAGA CAGCAAAGAA TATCACTGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
    GTCCTAATTC CCGAAATCAA CCTCAATGAC ACGTTTGACA CTTTTGCCTT GGATTTTTCC 
    CGGGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTAACAG CTCCCAACCC TCCCGCGATT 
    AGAGAAGAGC TCTGCACCGC TTCCTACGAC ACCATCACCG TCCACTGGAC CTCAGAGGAC 
    GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAATGTT 
    GTCAGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_006528739.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528802.1 Mus musculus midline 1 (Mid1), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID OMu56599
    Clone ID Related Accession (Same CDS sequence) XM_006528739.3 , XM_006528739.4
    Accession Version XM_006528739.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006528802.1
    CDS283..1305
    Translation

    Target ORF information:

    RefSeq Version XM_006528739.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006528739.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( NM_001290504.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277433.1 Mus musculus midline 1 (Mid1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OMu13850
    Clone ID Related Accession (Same CDS sequence) XM_011247789.2 , NM_001290504.1 , XM_011247789.3 , NM_001290505.1 , XM_030251241.1
    Accession Version NM_001290504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-01
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672015.7 and CR478112.2. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 3 and 4 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF026565.1, AK145142.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849387 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277433.1
    CDS124..2127
    Translation

    Target ORF information:

    RefSeq Version NM_001290504.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( NM_001290505.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277434.1 Mus musculus midline 1 (Mid1), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OMu13850
    Clone ID Related Accession (Same CDS sequence) XM_011247789.2 , NM_001290504.1 , XM_011247789.3 , NM_001290505.1 , XM_030251241.1
    Accession Version NM_001290505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-01
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672015.7 and CR478112.2. On Mar 13, 2014 this sequence version replaced XM_006528731.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 3 and 4 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145142.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277434.1
    CDS348..2351
    Translation

    Target ORF information:

    RefSeq Version NM_001290505.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001290505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( NM_010797.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034927.2 Mus musculus midline 1 (Mid1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version NM_010797.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-01
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671865.11, AL672015.7 and CR478112.2. On Mar 13, 2014 this sequence version replaced NM_010797.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: Y14848.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_034927.2
    CDS231..2273
    Translation

    Target ORF information:

    RefSeq Version NM_010797.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_010797.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_011247789.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246091.1 Mus musculus midline 1 (Mid1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMu13850
    Clone ID Related Accession (Same CDS sequence) XM_011247789.2 , NM_001290504.1 , XM_011247789.3 , NM_001290505.1 , XM_030251241.1
    Accession Version XM_011247789.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246091.1
    CDS843..2846
    Translation

    Target ORF information:

    RefSeq Version XM_011247789.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011247789.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318409.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173898.1 Mus musculus midline 1 (Mid1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version XM_017318409.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173898.1
    CDS854..2896
    Translation

    Target ORF information:

    RefSeq Version XM_017318409.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318410.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173899.1 Mus musculus midline 1 (Mid1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version XM_017318410.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173899.1
    CDS407..2449
    Translation

    Target ORF information:

    RefSeq Version XM_017318410.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318408.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173897.1 Mus musculus midline 1 (Mid1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version XM_017318408.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173897.1
    CDS371..2413
    Translation

    Target ORF information:

    RefSeq Version XM_017318408.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_011247789.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246091.1 Mus musculus midline 1 (Mid1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMu13850
    Clone ID Related Accession (Same CDS sequence) XM_011247789.2 , NM_001290504.1 , XM_011247789.3 , NM_001290505.1 , XM_030251241.1
    Accession Version XM_011247789.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246091.1
    CDS839..2842
    Translation

    Target ORF information:

    RefSeq Version XM_011247789.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011247789.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_030251241.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107101.1 Mus musculus midline 1 (Mid1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OMu13850
    Clone ID Related Accession (Same CDS sequence) XM_011247789.2 , NM_001290504.1 , XM_011247789.3 , NM_001290505.1 , XM_030251241.1
    Accession Version XM_030251241.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107101.1
    CDS386..2389
    Translation

    Target ORF information:

    RefSeq Version XM_030251241.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_030251241.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTCTG TGTAACTCGG CGGACAGCTG GATGATCGTG 
    CCCAACATCA AGCAGAACCA CTACACCGTG CACGGCCTGC AAAGTGGCAC CAAGTATATC 
    TTCACGGTGA AGGCCATCAA CCAGGCGGGC AGCCGTAGCA GCGAGCCCGG AAAGCTGAAG 
    ACCAACAGTC AGCCGTTTAG ACTGGATCCC AAATCGGCTC ATCGCAAGCT GAAGGTGTCC 
    CACGACAACC TGACTGTCGA GCGCGACGAG TCGTCCTCTA AGAAGAGTCA CGCGCCGGAG 
    CGCTTCGCTG GTCAGGGGAG CTACGGAGTG GCTGGCAACG TGTTCATCGA CAGCGGCCGT 
    CACTACTGGG AAGTGGTCAC CAGCGGAAGC ACATGGTACG CCATCGGCCT GGCGTACAGA 
    TCGGCGCCGA AACACGAGTG GATCGGGAAG AACGCGGCGT CCTGGGCCCT CTGCCGCTGC 
    CACAACCACT GGGCGGTGCG ACACGACGGC AAGGAGACCC CCATCGCGCC GGCCCCTCAC 
    CTCAGGCGCG TCGGCGTCCT GCTGGACTAC GACAACGGCT CCATCGCCTT CTACGACGCT 
    CTGAGCTCCG TCCACCTCCA CACCTTCCAC GCGGCGCTCG CGCAGCCCGT GTGCCCCACC 
    TTCACCGTGT GGAACAAGTG TCTGACCATC GTCACGGGTC TGCCCATCCC GGACCATCTG 
    GACTGTACGG AGCAGCGACC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_030251244.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107104.1 Mus musculus midline 1 (Mid1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OMu13871
    Clone ID Related Accession (Same CDS sequence) NM_001290506.1 , XM_030251244.1
    Accession Version XM_030251244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AGTCTGTGTA ACTCGGCGGA CAGCTGGATG 
    ATCGTGCCCA ACATCAAGCA GAACCACTAC ACCGTGCACG GCCTGCAAAG TGGCACCAAG 
    TATATCTTCA CGGTGAAGGC CATCAACCAG GCGGGCAGCC GTAGCAGCGA GCCCGGAAAG 
    CTGAAGACCA ACAGTCAGCC GTTTAGACTG GATCCCAAAT CGGCTCATCG CAAGCTGAAG 
    GTGTCCCACG ACAACCTGAC TGTCGAGCGC GACGAGTCGT CCTCTAAGAA GAGTCACGCG 
    CCGGAGCGCT TCGCTGGTCA GGGGAGCTAC GGAGTGGCTG GCAACGTGTT CATCGACAGC 
    GGCCGTCACT ACTGGGAAGT GGTCACCAGC GGAAGCACAT GGTACGCCAT CGGCCTGGCG 
    TACAGATCGG CGCCGAAACA CGAGTGGATC GGGAAGAACG CGGCGTCCTG GGCCCTCTGC 
    CGCTGCCACA ACCACTGGGC GGTGCGACAC GACGGCAAGG AGACCCCCAT CGCGCCGGCC 
    CCTCACCTCA GGCGCGTCGG CGTCCTGCTG GACTACGACA ACGGCTCCAT CGCCTTCTAC 
    GACGCTCTGA GCTCCGTCCA CCTCCACACC TTCCACGCGG CGCTCGCGCA GCCCGTGTGC 
    CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACCATCGTCA CGGGTCTGCC CATCCCGGAC 
    CATCTGGACT GTACGGAGCA GCGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107104.1
    CDS836..2725
    Translation

    Target ORF information:

    RefSeq Version XM_030251244.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_030251244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AGTCTGTGTA ACTCGGCGGA CAGCTGGATG 
    ATCGTGCCCA ACATCAAGCA GAACCACTAC ACCGTGCACG GCCTGCAAAG TGGCACCAAG 
    TATATCTTCA CGGTGAAGGC CATCAACCAG GCGGGCAGCC GTAGCAGCGA GCCCGGAAAG 
    CTGAAGACCA ACAGTCAGCC GTTTAGACTG GATCCCAAAT CGGCTCATCG CAAGCTGAAG 
    GTGTCCCACG ACAACCTGAC TGTCGAGCGC GACGAGTCGT CCTCTAAGAA GAGTCACGCG 
    CCGGAGCGCT TCGCTGGTCA GGGGAGCTAC GGAGTGGCTG GCAACGTGTT CATCGACAGC 
    GGCCGTCACT ACTGGGAAGT GGTCACCAGC GGAAGCACAT GGTACGCCAT CGGCCTGGCG 
    TACAGATCGG CGCCGAAACA CGAGTGGATC GGGAAGAACG CGGCGTCCTG GGCCCTCTGC 
    CGCTGCCACA ACCACTGGGC GGTGCGACAC GACGGCAAGG AGACCCCCAT CGCGCCGGCC 
    CCTCACCTCA GGCGCGTCGG CGTCCTGCTG GACTACGACA ACGGCTCCAT CGCCTTCTAC 
    GACGCTCTGA GCTCCGTCCA CCTCCACACC TTCCACGCGG CGCTCGCGCA GCCCGTGTGC 
    CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACCATCGTCA CGGGTCTGCC CATCCCGGAC 
    CATCTGGACT GTACGGAGCA GCGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318410.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173899.1 Mus musculus midline 1 (Mid1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version XM_017318410.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173899.1
    CDS434..2476
    Translation

    Target ORF information:

    RefSeq Version XM_017318410.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318410.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318408.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173897.1 Mus musculus midline 1 (Mid1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version XM_017318408.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173897.1
    CDS372..2414
    Translation

    Target ORF information:

    RefSeq Version XM_017318408.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318408.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318409.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173898.1 Mus musculus midline 1 (Mid1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMu13885
    Clone ID Related Accession (Same CDS sequence) NM_010797.3 , XM_017318410.2 , XM_017318409.1 , XM_017318410.1 , XM_017318408.1 , XM_017318408.2 , XM_017318409.2
    Accession Version XM_017318409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173898.1
    CDS846..2888
    Translation

    Target ORF information:

    RefSeq Version XM_017318409.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAACGTG GCCTGTGACG GCACCTGTCT CCTCGGCTCC 
    GCAGGTCTGT GTAACTCGGC GGACAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
    TACACCGTGC ACGGCCTGCA AAGTGGCACC AAGTATATCT TCACGGTGAA GGCCATCAAC 
    CAGGCGGGCA GCCGTAGCAG CGAGCCCGGA AAGCTGAAGA CCAACAGTCA GCCGTTTAGA 
    CTGGATCCCA AATCGGCTCA TCGCAAGCTG AAGGTGTCCC ACGACAACCT GACTGTCGAG 
    CGCGACGAGT CGTCCTCTAA GAAGAGTCAC GCGCCGGAGC GCTTCGCTGG TCAGGGGAGC 
    TACGGAGTGG CTGGCAACGT GTTCATCGAC AGCGGCCGTC ACTACTGGGA AGTGGTCACC 
    AGCGGAAGCA CATGGTACGC CATCGGCCTG GCGTACAGAT CGGCGCCGAA ACACGAGTGG 
    ATCGGGAAGA ACGCGGCGTC CTGGGCCCTC TGCCGCTGCC ACAACCACTG GGCGGTGCGA 
    CACGACGGCA AGGAGACCCC CATCGCGCCG GCCCCTCACC TCAGGCGCGT CGGCGTCCTG 
    CTGGACTACG ACAACGGCTC CATCGCCTTC TACGACGCTC TGAGCTCCGT CCACCTCCAC 
    ACCTTCCACG CGGCGCTCGC GCAGCCCGTG TGCCCCACCT TCACCGTGTG GAACAAGTGT 
    CTGACCATCG TCACGGGTCT GCCCATCCCG GACCATCTGG ACTGTACGGA GCAGCGACCT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318411.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173900.1 Mus musculus midline 1 (Mid1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OMu83087
    Clone ID Related Accession (Same CDS sequence) XM_017318411.2 , XM_017318411.1
    Accession Version XM_017318411.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAACG TGGCCTGTGA CGGCACCTGT 
    CTCCTCGGCT CCGCAGGTCT GTGTAACTCG GCGGACAGCT GGATGATCGT GCCCAACATC 
    AAGCAGAACC ACTACACCGT GCACGGCCTG CAAAGTGGCA CCAAGTATAT CTTCACGGTG 
    AAGGCCATCA ACCAGGCGGG CAGCCGTAGC AGCGAGCCCG GAAAGCTGAA GACCAACAGT 
    CAGCCGTTTA GACTGGATCC CAAATCGGCT CATCGCAAGC TGAAGGTGTC CCACGACAAC 
    CTGACTGTCG AGCGCGACGA GTCGTCCTCT AAGAAGAGTC ACGCGCCGGA GCGCTTCGCT 
    GGTCAGGGGA GCTACGGAGT GGCTGGCAAC GTGTTCATCG ACAGCGGCCG TCACTACTGG 
    GAAGTGGTCA CCAGCGGAAG CACATGGTAC GCCATCGGCC TGGCGTACAG ATCGGCGCCG 
    AAACACGAGT GGATCGGGAA GAACGCGGCG TCCTGGGCCC TCTGCCGCTG CCACAACCAC 
    TGGGCGGTGC GACACGACGG CAAGGAGACC CCCATCGCGC CGGCCCCTCA CCTCAGGCGC 
    GTCGGCGTCC TGCTGGACTA CGACAACGGC TCCATCGCCT TCTACGACGC TCTGAGCTCC 
    GTCCACCTCC ACACCTTCCA CGCGGCGCTC GCGCAGCCCG TGTGCCCCAC CTTCACCGTG 
    TGGAACAAGT GTCTGACCAT CGTCACGGGT CTGCCCATCC CGGACCATCT GGACTGTACG 
    GAGCAGCGAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173900.1
    CDS372..2306
    Translation

    Target ORF information:

    RefSeq Version XM_017318411.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318411.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAACG TGGCCTGTGA CGGCACCTGT 
    CTCCTCGGCT CCGCAGGTCT GTGTAACTCG GCGGACAGCT GGATGATCGT GCCCAACATC 
    AAGCAGAACC ACTACACCGT GCACGGCCTG CAAAGTGGCA CCAAGTATAT CTTCACGGTG 
    AAGGCCATCA ACCAGGCGGG CAGCCGTAGC AGCGAGCCCG GAAAGCTGAA GACCAACAGT 
    CAGCCGTTTA GACTGGATCC CAAATCGGCT CATCGCAAGC TGAAGGTGTC CCACGACAAC 
    CTGACTGTCG AGCGCGACGA GTCGTCCTCT AAGAAGAGTC ACGCGCCGGA GCGCTTCGCT 
    GGTCAGGGGA GCTACGGAGT GGCTGGCAAC GTGTTCATCG ACAGCGGCCG TCACTACTGG 
    GAAGTGGTCA CCAGCGGAAG CACATGGTAC GCCATCGGCC TGGCGTACAG ATCGGCGCCG 
    AAACACGAGT GGATCGGGAA GAACGCGGCG TCCTGGGCCC TCTGCCGCTG CCACAACCAC 
    TGGGCGGTGC GACACGACGG CAAGGAGACC CCCATCGCGC CGGCCCCTCA CCTCAGGCGC 
    GTCGGCGTCC TGCTGGACTA CGACAACGGC TCCATCGCCT TCTACGACGC TCTGAGCTCC 
    GTCCACCTCC ACACCTTCCA CGCGGCGCTC GCGCAGCCCG TGTGCCCCAC CTTCACCGTG 
    TGGAACAAGT GTCTGACCAT CGTCACGGGT CTGCCCATCC CGGACCATCT GGACTGTACG 
    GAGCAGCGAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318411.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173900.1 Mus musculus midline 1 (Mid1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OMu83087
    Clone ID Related Accession (Same CDS sequence) XM_017318411.2 , XM_017318411.1
    Accession Version XM_017318411.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAACG TGGCCTGTGA CGGCACCTGT 
    CTCCTCGGCT CCGCAGGTCT GTGTAACTCG GCGGACAGCT GGATGATCGT GCCCAACATC 
    AAGCAGAACC ACTACACCGT GCACGGCCTG CAAAGTGGCA CCAAGTATAT CTTCACGGTG 
    AAGGCCATCA ACCAGGCGGG CAGCCGTAGC AGCGAGCCCG GAAAGCTGAA GACCAACAGT 
    CAGCCGTTTA GACTGGATCC CAAATCGGCT CATCGCAAGC TGAAGGTGTC CCACGACAAC 
    CTGACTGTCG AGCGCGACGA GTCGTCCTCT AAGAAGAGTC ACGCGCCGGA GCGCTTCGCT 
    GGTCAGGGGA GCTACGGAGT GGCTGGCAAC GTGTTCATCG ACAGCGGCCG TCACTACTGG 
    GAAGTGGTCA CCAGCGGAAG CACATGGTAC GCCATCGGCC TGGCGTACAG ATCGGCGCCG 
    AAACACGAGT GGATCGGGAA GAACGCGGCG TCCTGGGCCC TCTGCCGCTG CCACAACCAC 
    TGGGCGGTGC GACACGACGG CAAGGAGACC CCCATCGCGC CGGCCCCTCA CCTCAGGCGC 
    GTCGGCGTCC TGCTGGACTA CGACAACGGC TCCATCGCCT TCTACGACGC TCTGAGCTCC 
    GTCCACCTCC ACACCTTCCA CGCGGCGCTC GCGCAGCCCG TGTGCCCCAC CTTCACCGTG 
    TGGAACAAGT GTCTGACCAT CGTCACGGGT CTGCCCATCC CGGACCATCT GGACTGTACG 
    GAGCAGCGAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173900.1
    CDS370..2304
    Translation

    Target ORF information:

    RefSeq Version XM_017318411.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAACG TGGCCTGTGA CGGCACCTGT 
    CTCCTCGGCT CCGCAGGTCT GTGTAACTCG GCGGACAGCT GGATGATCGT GCCCAACATC 
    AAGCAGAACC ACTACACCGT GCACGGCCTG CAAAGTGGCA CCAAGTATAT CTTCACGGTG 
    AAGGCCATCA ACCAGGCGGG CAGCCGTAGC AGCGAGCCCG GAAAGCTGAA GACCAACAGT 
    CAGCCGTTTA GACTGGATCC CAAATCGGCT CATCGCAAGC TGAAGGTGTC CCACGACAAC 
    CTGACTGTCG AGCGCGACGA GTCGTCCTCT AAGAAGAGTC ACGCGCCGGA GCGCTTCGCT 
    GGTCAGGGGA GCTACGGAGT GGCTGGCAAC GTGTTCATCG ACAGCGGCCG TCACTACTGG 
    GAAGTGGTCA CCAGCGGAAG CACATGGTAC GCCATCGGCC TGGCGTACAG ATCGGCGCCG 
    AAACACGAGT GGATCGGGAA GAACGCGGCG TCCTGGGCCC TCTGCCGCTG CCACAACCAC 
    TGGGCGGTGC GACACGACGG CAAGGAGACC CCCATCGCGC CGGCCCCTCA CCTCAGGCGC 
    GTCGGCGTCC TGCTGGACTA CGACAACGGC TCCATCGCCT TCTACGACGC TCTGAGCTCC 
    GTCCACCTCC ACACCTTCCA CGCGGCGCTC GCGCAGCCCG TGTGCCCCAC CTTCACCGTG 
    TGGAACAAGT GTCTGACCAT CGTCACGGGT CTGCCCATCC CGGACCATCT GGACTGTACG 
    GAGCAGCGAC CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318412.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173901.1 Mus musculus midline 1 (Mid1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OMu83088
    Clone ID Related Accession (Same CDS sequence) XM_017318412.2 , XM_017318412.1
    Accession Version XM_017318412.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AACGTGGCCT GTGACGGCAC CTGTCTCCTC 
    GGCTCCGCAG GTCTGTGTAA CTCGGCGGAC AGCTGGATGA TCGTGCCCAA CATCAAGCAG 
    AACCACTACA CCGTGCACGG CCTGCAAAGT GGCACCAAGT ATATCTTCAC GGTGAAGGCC 
    ATCAACCAGG CGGGCAGCCG TAGCAGCGAG CCCGGAAAGC TGAAGACCAA CAGTCAGCCG 
    TTTAGACTGG ATCCCAAATC GGCTCATCGC AAGCTGAAGG TGTCCCACGA CAACCTGACT 
    GTCGAGCGCG ACGAGTCGTC CTCTAAGAAG AGTCACGCGC CGGAGCGCTT CGCTGGTCAG 
    GGGAGCTACG GAGTGGCTGG CAACGTGTTC ATCGACAGCG GCCGTCACTA CTGGGAAGTG 
    GTCACCAGCG GAAGCACATG GTACGCCATC GGCCTGGCGT ACAGATCGGC GCCGAAACAC 
    GAGTGGATCG GGAAGAACGC GGCGTCCTGG GCCCTCTGCC GCTGCCACAA CCACTGGGCG 
    GTGCGACACG ACGGCAAGGA GACCCCCATC GCGCCGGCCC CTCACCTCAG GCGCGTCGGC 
    GTCCTGCTGG ACTACGACAA CGGCTCCATC GCCTTCTACG ACGCTCTGAG CTCCGTCCAC 
    CTCCACACCT TCCACGCGGC GCTCGCGCAG CCCGTGTGCC CCACCTTCAC CGTGTGGAAC 
    AAGTGTCTGA CCATCGTCAC GGGTCTGCCC ATCCCGGACC ATCTGGACTG TACGGAGCAG 
    CGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173901.1
    CDS370..2298
    Translation

    Target ORF information:

    RefSeq Version XM_017318412.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318412.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AACGTGGCCT GTGACGGCAC CTGTCTCCTC 
    GGCTCCGCAG GTCTGTGTAA CTCGGCGGAC AGCTGGATGA TCGTGCCCAA CATCAAGCAG 
    AACCACTACA CCGTGCACGG CCTGCAAAGT GGCACCAAGT ATATCTTCAC GGTGAAGGCC 
    ATCAACCAGG CGGGCAGCCG TAGCAGCGAG CCCGGAAAGC TGAAGACCAA CAGTCAGCCG 
    TTTAGACTGG ATCCCAAATC GGCTCATCGC AAGCTGAAGG TGTCCCACGA CAACCTGACT 
    GTCGAGCGCG ACGAGTCGTC CTCTAAGAAG AGTCACGCGC CGGAGCGCTT CGCTGGTCAG 
    GGGAGCTACG GAGTGGCTGG CAACGTGTTC ATCGACAGCG GCCGTCACTA CTGGGAAGTG 
    GTCACCAGCG GAAGCACATG GTACGCCATC GGCCTGGCGT ACAGATCGGC GCCGAAACAC 
    GAGTGGATCG GGAAGAACGC GGCGTCCTGG GCCCTCTGCC GCTGCCACAA CCACTGGGCG 
    GTGCGACACG ACGGCAAGGA GACCCCCATC GCGCCGGCCC CTCACCTCAG GCGCGTCGGC 
    GTCCTGCTGG ACTACGACAA CGGCTCCATC GCCTTCTACG ACGCTCTGAG CTCCGTCCAC 
    CTCCACACCT TCCACGCGGC GCTCGCGCAG CCCGTGTGCC CCACCTTCAC CGTGTGGAAC 
    AAGTGTCTGA CCATCGTCAC GGGTCTGCCC ATCCCGGACC ATCTGGACTG TACGGAGCAG 
    CGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318412.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173901.1 Mus musculus midline 1 (Mid1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OMu83088
    Clone ID Related Accession (Same CDS sequence) XM_017318412.2 , XM_017318412.1
    Accession Version XM_017318412.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AACGTGGCCT GTGACGGCAC CTGTCTCCTC 
    GGCTCCGCAG GTCTGTGTAA CTCGGCGGAC AGCTGGATGA TCGTGCCCAA CATCAAGCAG 
    AACCACTACA CCGTGCACGG CCTGCAAAGT GGCACCAAGT ATATCTTCAC GGTGAAGGCC 
    ATCAACCAGG CGGGCAGCCG TAGCAGCGAG CCCGGAAAGC TGAAGACCAA CAGTCAGCCG 
    TTTAGACTGG ATCCCAAATC GGCTCATCGC AAGCTGAAGG TGTCCCACGA CAACCTGACT 
    GTCGAGCGCG ACGAGTCGTC CTCTAAGAAG AGTCACGCGC CGGAGCGCTT CGCTGGTCAG 
    GGGAGCTACG GAGTGGCTGG CAACGTGTTC ATCGACAGCG GCCGTCACTA CTGGGAAGTG 
    GTCACCAGCG GAAGCACATG GTACGCCATC GGCCTGGCGT ACAGATCGGC GCCGAAACAC 
    GAGTGGATCG GGAAGAACGC GGCGTCCTGG GCCCTCTGCC GCTGCCACAA CCACTGGGCG 
    GTGCGACACG ACGGCAAGGA GACCCCCATC GCGCCGGCCC CTCACCTCAG GCGCGTCGGC 
    GTCCTGCTGG ACTACGACAA CGGCTCCATC GCCTTCTACG ACGCTCTGAG CTCCGTCCAC 
    CTCCACACCT TCCACGCGGC GCTCGCGCAG CCCGTGTGCC CCACCTTCAC CGTGTGGAAC 
    AAGTGTCTGA CCATCGTCAC GGGTCTGCCC ATCCCGGACC ATCTGGACTG TACGGAGCAG 
    CGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173901.1
    CDS370..2298
    Translation

    Target ORF information:

    RefSeq Version XM_017318412.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGCAAA ACTTGGAGAG TAATCTCACC AATCTTATTA AGAGAAACAC AGAACTGGAG 
    ACTCTTTTGG CTAAACTCAT CCAAACTTGT CAACATGTTG AAGTCAATGC ATCCCGTCAA 
    GAAGCCAAAC TGACAGAAGA ATGTGATCTT CTCATTGAAA TCATTCAGCA ACGAAGACAA 
    ATTATTGGAA CAAAGATTAA AGAAGGCAAG GTGATCAGGC TCCGCAAGTT AGCTCAGCAG 
    ATTGCAAACT GTAAACAGTG CCTTGAGAGG TCTGCATCGC TCATCTCGCA AGCGGAGCAC 
    TCGCTGAAGG AAAATGACCA CGCCCGTTTT CTACAGACAG CAAAGAATAT CACTGAGAGA 
    GTCTCCATGG CAACTGCATC CTCCCAGGTC CTAATTCCCG AAATCAACCT CAATGACACG 
    TTTGACACTT TTGCCTTGGA TTTTTCCCGG GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
    CTAACAGCTC CCAACCCTCC CGCGATTAGA GAAGAGCTCT GCACCGCTTC CTACGACACC 
    ATCACCGTCC ACTGGACCTC AGAGGACGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
    ACCATATTCA CCGGACAAGC CAATGTTGTC AACGTGGCCT GTGACGGCAC CTGTCTCCTC 
    GGCTCCGCAG GTCTGTGTAA CTCGGCGGAC AGCTGGATGA TCGTGCCCAA CATCAAGCAG 
    AACCACTACA CCGTGCACGG CCTGCAAAGT GGCACCAAGT ATATCTTCAC GGTGAAGGCC 
    ATCAACCAGG CGGGCAGCCG TAGCAGCGAG CCCGGAAAGC TGAAGACCAA CAGTCAGCCG 
    TTTAGACTGG ATCCCAAATC GGCTCATCGC AAGCTGAAGG TGTCCCACGA CAACCTGACT 
    GTCGAGCGCG ACGAGTCGTC CTCTAAGAAG AGTCACGCGC CGGAGCGCTT CGCTGGTCAG 
    GGGAGCTACG GAGTGGCTGG CAACGTGTTC ATCGACAGCG GCCGTCACTA CTGGGAAGTG 
    GTCACCAGCG GAAGCACATG GTACGCCATC GGCCTGGCGT ACAGATCGGC GCCGAAACAC 
    GAGTGGATCG GGAAGAACGC GGCGTCCTGG GCCCTCTGCC GCTGCCACAA CCACTGGGCG 
    GTGCGACACG ACGGCAAGGA GACCCCCATC GCGCCGGCCC CTCACCTCAG GCGCGTCGGC 
    GTCCTGCTGG ACTACGACAA CGGCTCCATC GCCTTCTACG ACGCTCTGAG CTCCGTCCAC 
    CTCCACACCT TCCACGCGGC GCTCGCGCAG CCCGTGTGCC CCACCTTCAC CGTGTGGAAC 
    AAGTGTCTGA CCATCGTCAC GGGTCTGCCC ATCCCGGACC ATCTGGACTG TACGGAGCAG 
    CGACCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318413.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173902.1 Mus musculus midline 1 (Mid1), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OMu83089
    Clone ID Related Accession (Same CDS sequence) XM_017318413.1 , XM_017318413.2
    Accession Version XM_017318413.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTGAG TATCTCTCGG CTGATTCCCT TCCGCCTCTC 
    CGTTTTCCAA TGTTCAATGT CCAAATAATC TGCCATTCTG CAGACGTGGC CTGTGACGGC 
    ACCTGTCTCC TCGGCTCCGC AGGTCTGTGT AACTCGGCGG ACAGCTGGAT GATCGTGCCC 
    AACATCAAGC AGAACCACTA CACCGTGCAC GGCCTGCAAA GTGGCACCAA GTATATCTTC 
    ACGGTGAAGG CCATCAACCA GGCGGGCAGC CGTAGCAGCG AGCCCGGAAA GCTGAAGACC 
    AACAGTCAGC CGTTTAGACT GGATCCCAAA TCGGCTCATC GCAAGCTGAA GGTGTCCCAC 
    GACAACCTGA CTGTCGAGCG CGACGAGTCG TCCTCTAAGA AGAGTCACGC GCCGGAGCGC 
    TTCGCTGGTC AGGGGAGCTA CGGAGTGGCT GGCAACGTGT TCATCGACAG CGGCCGTCAC 
    TACTGGGAAG TGGTCACCAG CGGAAGCACA TGGTACGCCA TCGGCCTGGC GTACAGATCG 
    GCGCCGAAAC ACGAGTGGAT CGGGAAGAAC GCGGCGTCCT GGGCCCTCTG CCGCTGCCAC 
    AACCACTGGG CGGTGCGACA CGACGGCAAG GAGACCCCCA TCGCGCCGGC CCCTCACCTC 
    AGGCGCGTCG GCGTCCTGCT GGACTACGAC AACGGCTCCA TCGCCTTCTA CGACGCTCTG 
    AGCTCCGTCC ACCTCCACAC CTTCCACGCG GCGCTCGCGC AGCCCGTGTG CCCCACCTTC 
    ACCGTGTGGA ACAAGTGTCT GACCATCGTC ACGGGTCTGC CCATCCCGGA CCATCTGGAC 
    TGTACGGAGC AGCGACCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173902.1
    CDS49..2169
    Translation

    Target ORF information:

    RefSeq Version XM_017318413.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTGAG TATCTCTCGG CTGATTCCCT TCCGCCTCTC 
    CGTTTTCCAA TGTTCAATGT CCAAATAATC TGCCATTCTG CAGACGTGGC CTGTGACGGC 
    ACCTGTCTCC TCGGCTCCGC AGGTCTGTGT AACTCGGCGG ACAGCTGGAT GATCGTGCCC 
    AACATCAAGC AGAACCACTA CACCGTGCAC GGCCTGCAAA GTGGCACCAA GTATATCTTC 
    ACGGTGAAGG CCATCAACCA GGCGGGCAGC CGTAGCAGCG AGCCCGGAAA GCTGAAGACC 
    AACAGTCAGC CGTTTAGACT GGATCCCAAA TCGGCTCATC GCAAGCTGAA GGTGTCCCAC 
    GACAACCTGA CTGTCGAGCG CGACGAGTCG TCCTCTAAGA AGAGTCACGC GCCGGAGCGC 
    TTCGCTGGTC AGGGGAGCTA CGGAGTGGCT GGCAACGTGT TCATCGACAG CGGCCGTCAC 
    TACTGGGAAG TGGTCACCAG CGGAAGCACA TGGTACGCCA TCGGCCTGGC GTACAGATCG 
    GCGCCGAAAC ACGAGTGGAT CGGGAAGAAC GCGGCGTCCT GGGCCCTCTG CCGCTGCCAC 
    AACCACTGGG CGGTGCGACA CGACGGCAAG GAGACCCCCA TCGCGCCGGC CCCTCACCTC 
    AGGCGCGTCG GCGTCCTGCT GGACTACGAC AACGGCTCCA TCGCCTTCTA CGACGCTCTG 
    AGCTCCGTCC ACCTCCACAC CTTCCACGCG GCGCTCGCGC AGCCCGTGTG CCCCACCTTC 
    ACCGTGTGGA ACAAGTGTCT GACCATCGTC ACGGGTCTGC CCATCCCGGA CCATCTGGAC 
    TGTACGGAGC AGCGACCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_017318413.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173902.1 Mus musculus midline 1 (Mid1), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID OMu83089
    Clone ID Related Accession (Same CDS sequence) XM_017318413.1 , XM_017318413.2
    Accession Version XM_017318413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2121bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTGAG TATCTCTCGG CTGATTCCCT TCCGCCTCTC 
    CGTTTTCCAA TGTTCAATGT CCAAATAATC TGCCATTCTG CAGACGTGGC CTGTGACGGC 
    ACCTGTCTCC TCGGCTCCGC AGGTCTGTGT AACTCGGCGG ACAGCTGGAT GATCGTGCCC 
    AACATCAAGC AGAACCACTA CACCGTGCAC GGCCTGCAAA GTGGCACCAA GTATATCTTC 
    ACGGTGAAGG CCATCAACCA GGCGGGCAGC CGTAGCAGCG AGCCCGGAAA GCTGAAGACC 
    AACAGTCAGC CGTTTAGACT GGATCCCAAA TCGGCTCATC GCAAGCTGAA GGTGTCCCAC 
    GACAACCTGA CTGTCGAGCG CGACGAGTCG TCCTCTAAGA AGAGTCACGC GCCGGAGCGC 
    TTCGCTGGTC AGGGGAGCTA CGGAGTGGCT GGCAACGTGT TCATCGACAG CGGCCGTCAC 
    TACTGGGAAG TGGTCACCAG CGGAAGCACA TGGTACGCCA TCGGCCTGGC GTACAGATCG 
    GCGCCGAAAC ACGAGTGGAT CGGGAAGAAC GCGGCGTCCT GGGCCCTCTG CCGCTGCCAC 
    AACCACTGGG CGGTGCGACA CGACGGCAAG GAGACCCCCA TCGCGCCGGC CCCTCACCTC 
    AGGCGCGTCG GCGTCCTGCT GGACTACGAC AACGGCTCCA TCGCCTTCTA CGACGCTCTG 
    AGCTCCGTCC ACCTCCACAC CTTCCACGCG GCGCTCGCGC AGCCCGTGTG CCCCACCTTC 
    ACCGTGTGGA ACAAGTGTCT GACCATCGTC ACGGGTCTGC CCATCCCGGA CCATCTGGAC 
    TGTACGGAGC AGCGACCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173902.1
    CDS48..2168
    Translation

    Target ORF information:

    RefSeq Version XM_017318413.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017318413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTCA ATGCATCCCG TCAAGAAGCC 
    AAACTGACAG AAGAATGTGA TCTTCTCATT GAAATCATTC AGCAACGAAG ACAAATTATT 
    GGAACAAAGA TTAAAGAAGG CAAGGTGATC AGGCTCCGCA AGTTAGCTCA GCAGATTGCA 
    AACTGTAAAC AGTGCCTTGA GAGGTCTGCA TCGCTCATCT CGCAAGCGGA GCACTCGCTG 
    AAGGAAAATG ACCACGCCCG TTTTCTACAG ACAGCAAAGA ATATCACTGA GAGAGTCTCC 
    ATGGCAACTG CATCCTCCCA GGTCCTAATT CCCGAAATCA ACCTCAATGA CACGTTTGAC 
    ACTTTTGCCT TGGATTTTTC CCGGGAGAAG AAACTGCTAG AATGTCTGGA TTACCTAACA 
    GCTCCCAACC CTCCCGCGAT TAGAGAAGAG CTCTGCACCG CTTCCTACGA CACCATCACC 
    GTCCACTGGA CCTCAGAGGA CGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
    TTCACCGGAC AAGCCAATGT TGTCAGTGAG TATCTCTCGG CTGATTCCCT TCCGCCTCTC 
    CGTTTTCCAA TGTTCAATGT CCAAATAATC TGCCATTCTG CAGACGTGGC CTGTGACGGC 
    ACCTGTCTCC TCGGCTCCGC AGGTCTGTGT AACTCGGCGG ACAGCTGGAT GATCGTGCCC 
    AACATCAAGC AGAACCACTA CACCGTGCAC GGCCTGCAAA GTGGCACCAA GTATATCTTC 
    ACGGTGAAGG CCATCAACCA GGCGGGCAGC CGTAGCAGCG AGCCCGGAAA GCTGAAGACC 
    AACAGTCAGC CGTTTAGACT GGATCCCAAA TCGGCTCATC GCAAGCTGAA GGTGTCCCAC 
    GACAACCTGA CTGTCGAGCG CGACGAGTCG TCCTCTAAGA AGAGTCACGC GCCGGAGCGC 
    TTCGCTGGTC AGGGGAGCTA CGGAGTGGCT GGCAACGTGT TCATCGACAG CGGCCGTCAC 
    TACTGGGAAG TGGTCACCAG CGGAAGCACA TGGTACGCCA TCGGCCTGGC GTACAGATCG 
    GCGCCGAAAC ACGAGTGGAT CGGGAAGAAC GCGGCGTCCT GGGCCCTCTG CCGCTGCCAC 
    AACCACTGGG CGGTGCGACA CGACGGCAAG GAGACCCCCA TCGCGCCGGC CCCTCACCTC 
    AGGCGCGTCG GCGTCCTGCT GGACTACGAC AACGGCTCCA TCGCCTTCTA CGACGCTCTG 
    AGCTCCGTCC ACCTCCACAC CTTCCACGCG GCGCTCGCGC AGCCCGTGTG CCCCACCTTC 
    ACCGTGTGGA ACAAGTGTCT GACCATCGTC ACGGGTCTGC CCATCCCGGA CCATCTGGAC 
    TGTACGGAGC AGCGACCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_030251243.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107103.1 Mus musculus midline 1 (Mid1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OMu90363
    Clone ID Related Accession (Same CDS sequence) XM_030251243.1 , XM_030251242.1
    Accession Version XM_030251243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAGTC TGTGTAACTC GGCGGACAGC 
    TGGATGATCG TGCCCAACAT CAAGCAGAAC CACTACACCG TGCACGGCCT GCAAAGTGGC 
    ACCAAGTATA TCTTCACGGT GAAGGCCATC AACCAGGCGG GCAGCCGTAG CAGCGAGCCC 
    GGAAAGCTGA AGACCAACAG TCAGCCGTTT AGACTGGATC CCAAATCGGC TCATCGCAAG 
    CTGAAGGTGT CCCACGACAA CCTGACTGTC GAGCGCGACG AGTCGTCCTC TAAGAAGAGT 
    CACGCGCCGG AGCGCTTCGC TGGTCAGGGG AGCTACGGAG TGGCTGGCAA CGTGTTCATC 
    GACAGCGGCC GTCACTACTG GGAAGTGGTC ACCAGCGGAA GCACATGGTA CGCCATCGGC 
    CTGGCGTACA GATCGGCGCC GAAACACGAG TGGATCGGGA AGAACGCGGC GTCCTGGGCC 
    CTCTGCCGCT GCCACAACCA CTGGGCGGTG CGACACGACG GCAAGGAGAC CCCCATCGCG 
    CCGGCCCCTC ACCTCAGGCG CGTCGGCGTC CTGCTGGACT ACGACAACGG CTCCATCGCC 
    TTCTACGACG CTCTGAGCTC CGTCCACCTC CACACCTTCC ACGCGGCGCT CGCGCAGCCC 
    GTGTGCCCCA CCTTCACCGT GTGGAACAAG TGTCTGACCA TCGTCACGGG TCTGCCCATC 
    CCGGACCATC TGGACTGTAC GGAGCAGCGA CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107103.1
    CDS838..2733
    Translation

    Target ORF information:

    RefSeq Version XM_030251243.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_030251243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAGTC TGTGTAACTC GGCGGACAGC 
    TGGATGATCG TGCCCAACAT CAAGCAGAAC CACTACACCG TGCACGGCCT GCAAAGTGGC 
    ACCAAGTATA TCTTCACGGT GAAGGCCATC AACCAGGCGG GCAGCCGTAG CAGCGAGCCC 
    GGAAAGCTGA AGACCAACAG TCAGCCGTTT AGACTGGATC CCAAATCGGC TCATCGCAAG 
    CTGAAGGTGT CCCACGACAA CCTGACTGTC GAGCGCGACG AGTCGTCCTC TAAGAAGAGT 
    CACGCGCCGG AGCGCTTCGC TGGTCAGGGG AGCTACGGAG TGGCTGGCAA CGTGTTCATC 
    GACAGCGGCC GTCACTACTG GGAAGTGGTC ACCAGCGGAA GCACATGGTA CGCCATCGGC 
    CTGGCGTACA GATCGGCGCC GAAACACGAG TGGATCGGGA AGAACGCGGC GTCCTGGGCC 
    CTCTGCCGCT GCCACAACCA CTGGGCGGTG CGACACGACG GCAAGGAGAC CCCCATCGCG 
    CCGGCCCCTC ACCTCAGGCG CGTCGGCGTC CTGCTGGACT ACGACAACGG CTCCATCGCC 
    TTCTACGACG CTCTGAGCTC CGTCCACCTC CACACCTTCC ACGCGGCGCT CGCGCAGCCC 
    GTGTGCCCCA CCTTCACCGT GTGGAACAAG TGTCTGACCA TCGTCACGGG TCTGCCCATC 
    CCGGACCATC TGGACTGTAC GGAGCAGCGA CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Mid1 ( XM_030251242.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107102.1 Mus musculus midline 1 (Mid1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OMu90363
    Clone ID Related Accession (Same CDS sequence) XM_030251243.1 , XM_030251242.1
    Accession Version XM_030251242.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAGTC TGTGTAACTC GGCGGACAGC 
    TGGATGATCG TGCCCAACAT CAAGCAGAAC CACTACACCG TGCACGGCCT GCAAAGTGGC 
    ACCAAGTATA TCTTCACGGT GAAGGCCATC AACCAGGCGG GCAGCCGTAG CAGCGAGCCC 
    GGAAAGCTGA AGACCAACAG TCAGCCGTTT AGACTGGATC CCAAATCGGC TCATCGCAAG 
    CTGAAGGTGT CCCACGACAA CCTGACTGTC GAGCGCGACG AGTCGTCCTC TAAGAAGAGT 
    CACGCGCCGG AGCGCTTCGC TGGTCAGGGG AGCTACGGAG TGGCTGGCAA CGTGTTCATC 
    GACAGCGGCC GTCACTACTG GGAAGTGGTC ACCAGCGGAA GCACATGGTA CGCCATCGGC 
    CTGGCGTACA GATCGGCGCC GAAACACGAG TGGATCGGGA AGAACGCGGC GTCCTGGGCC 
    CTCTGCCGCT GCCACAACCA CTGGGCGGTG CGACACGACG GCAAGGAGAC CCCCATCGCG 
    CCGGCCCCTC ACCTCAGGCG CGTCGGCGTC CTGCTGGACT ACGACAACGG CTCCATCGCC 
    TTCTACGACG CTCTGAGCTC CGTCCACCTC CACACCTTCC ACGCGGCGCT CGCGCAGCCC 
    GTGTGCCCCA CCTTCACCGT GTGGAACAAG TGTCTGACCA TCGTCACGGG TCTGCCCATC 
    CCGGACCATC TGGACTGTAC GGAGCAGCGA CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107102.1
    CDS483..2378
    Translation

    Target ORF information:

    RefSeq Version XM_030251242.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus midline 1 (Mid1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_030251242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT 
    CTCCTGCTTC CCTGCGCACA TAGCCTCTGC TTCAACTGTG CCCACCGAAT CCTGGTCTCT 
    CACTGTGCCA CCAACGAGCC TGTGGAGTCC ATCAACGCCT TCCAGTGCCC CACCTGCCGG 
    CATGTCATCA CGCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTCCAA 
    AACATCATTG ACAGATTTCA GAAAGCATCT GTGAGCGGGC CCAACTCTCC CAGTGAGACC 
    CGCAGGGAAC GGGCCTTTGA CGCCAACACC ATGTCCTCTG CCGAGAAGGT TCTCTGCCAG 
    TTCTGTGACC AGGATCCTGC CCAGGATGCT GTGAAGACTT GCGTTACTTG TGAAGTGTCC 
    TACTGTGATG AGTGCCTGAA AGCCACTCAT CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
    CTGATTGAGC CAATCCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
    CAAAACTTGG AGAGTAATCT CACCAATCTT ATTAAGAGAA ACACAGAACT GGAGACTCTT 
    TTGGCTAAAC TCATCCAAAC TTGTCAACAT GTTGAAGTGA TCAGGCTCCG CAAGTTAGCT 
    CAGCAGATTG CAAACTGTAA ACAGTGCCTT GAGAGGTCTG CATCGCTCAT CTCGCAAGCG 
    GAGCACTCGC TGAAGGAAAA TGACCACGCC CGTTTTCTAC AGACAGCAAA GAATATCACT 
    GAGAGAGTCT CCATGGCAAC TGCATCCTCC CAGGTCCTAA TTCCCGAAAT CAACCTCAAT 
    GACACGTTTG ACACTTTTGC CTTGGATTTT TCCCGGGAGA AGAAACTGCT AGAATGTCTG 
    GATTACCTAA CAGCTCCCAA CCCTCCCGCG ATTAGAGAAG AGCTCTGCAC CGCTTCCTAC 
    GACACCATCA CCGTCCACTG GACCTCAGAG GACGAGTTCA GCGTGGTCTC CTACGAGCTC 
    CAGTACACCA TATTCACCGG ACAAGCCAAT GTTGTCAGTC TGTGTAACTC GGCGGACAGC 
    TGGATGATCG TGCCCAACAT CAAGCAGAAC CACTACACCG TGCACGGCCT GCAAAGTGGC 
    ACCAAGTATA TCTTCACGGT GAAGGCCATC AACCAGGCGG GCAGCCGTAG CAGCGAGCCC 
    GGAAAGCTGA AGACCAACAG TCAGCCGTTT AGACTGGATC CCAAATCGGC TCATCGCAAG 
    CTGAAGGTGT CCCACGACAA CCTGACTGTC GAGCGCGACG AGTCGTCCTC TAAGAAGAGT 
    CACGCGCCGG AGCGCTTCGC TGGTCAGGGG AGCTACGGAG TGGCTGGCAA CGTGTTCATC 
    GACAGCGGCC GTCACTACTG GGAAGTGGTC ACCAGCGGAA GCACATGGTA CGCCATCGGC 
    CTGGCGTACA GATCGGCGCC GAAACACGAG TGGATCGGGA AGAACGCGGC GTCCTGGGCC 
    CTCTGCCGCT GCCACAACCA CTGGGCGGTG CGACACGACG GCAAGGAGAC CCCCATCGCG 
    CCGGCCCCTC ACCTCAGGCG CGTCGGCGTC CTGCTGGACT ACGACAACGG CTCCATCGCC 
    TTCTACGACG CTCTGAGCTC CGTCCACCTC CACACCTTCC ACGCGGCGCT CGCGCAGCCC 
    GTGTGCCCCA CCTTCACCGT GTGGAACAAG TGTCTGACCA TCGTCACGGG TCTGCCCATC 
    CCGGACCATC TGGACTGTAC GGAGCAGCGA CCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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