Ucn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ucn2
Entrez Gene ID	171530
Full Name	urocortin 2
Synonyms	Ucn II,Ucn-2,ucn-II
General protein information	Preferred Names urocortin 2 Names urocortin-2 Urocortin II
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F2
Summary	This gene encodes a member of the corticotropin-releasing hormone peptide family that participates in coordinating autonomic, endocrine, and behavioral responses to stress. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional hormone. Mice lacking the encoded protein exhibit increased insulin sensitivity and were protected against fat-induced insulin resistance. In addition, female mice lacking the encoded protein exhibit a significant increase in the basal daily rhythms of adrenocorticotropic hormone and corticosterone, and a significant decrease in fluid intake and depressive-like behavior. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_145077.1	NP_659543.1	urocortin-2 preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ucn2	NP_659543.1
Rattus norvegicus (Norway rat)	Ucn2	NP_596876.2
Canis lupus familiaris (dog)	UCN2	XP_005632644.1
Bos taurus (cattle)	UCN2	XP_005223073.1
Homo sapiens (human)	UCN2	NP_149976.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of gonadotropins by urocortin 2 in gonadotropic tumor L?T2 cells.
Kageyama K, Murasawa S, Niioka K, Otsuka F, Yagi H, Daimon M
Neuroscience letters66063-67(2017 Nov)

Restoration of pharyngeal dilator muscle force in dystrophin-deficient (mdx) mice following co-treatment with neutralizing interleukin-6 receptor antibodies and urocortin 2.
Burns DP, Rowland J, Canavan L, Murphy KH, Brannock M, O'Malley D, O'Halloran KD, Edge D
Experimental physiology102(9)1177-1193(2017 Sep)

Gp91phox-derived Reactive Oxygen Species/Urocortin 2/Corticotropin-releasing Hormone Receptor Type 2 Play an Important Role in Long-term Ultraviolet A Eye Irradiation-induced Photoaging.
Hiramoto K, Yamate Y
Photochemistry and photobiology92(1)180-6(2016 Jan-Feb)

Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
Voltolini C, Battersby S, Novembri R, Torricelli M, Severi FM, Petraglia F, Norman JE
Endocrinology156(2)670-9(2015 Feb)

Posttranslational processing of human and mouse urocortin 2: characterization and bioactivity of gene products.
Vaughan JM, Donaldson CJ, Fischer WH, Perrin MH, Rivier JE, Sawchenko PE, Vale WW
Endocrinology154(4)1553-64(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that long-term UVA eye irradiation led to increased gp91phox-derived ROS in the brain and the increased expression of urocortin 2 and CRHR type 2, resulting in photoaging; however, further studies are needed to confirm these findings
Title: Gp91phox-derived Reactive Oxygen Species/Urocortin 2/Corticotropin-releasing Hormone Receptor Type 2 Play an Important Role in Long-term Ultraviolet A Eye Irradiation-induced Photoaging.

This study aims to investigate the cellular localization of CRF and UCNs in the ileum and to explore whether and how this cellular expression is altered in conditions of intestinal Schistosoma mansoni-induced inflammation.
Title: Expression of corticotropin-releasing factor and urocortins in the normal and Schistosoma mansoni-infected mouse ileum.

increased significantly in placenta approaching birth
Title: Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.

Leptin regulates corticotropin-releasing factor and urocortin 2/3 mRNA in hypothalamic neurons.
Title: Regulation of corticotropin-releasing factor and urocortin 2/3 mRNA by leptin in hypothalamic N39 cells.

Urocortin 2 autocrine/paracrine and pharmacologic effects to activate AMP-activated protein kinase in the heart.
Title: Urocortin 2 autocrine/paracrine and pharmacologic effects to activate AMP-activated protein kinase in the heart.

The following Ucn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ucn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18376	NM_145077.1 Latest version!	Mus musculus urocortin 2 (Ucn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ucn2 ( NM_145077.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659543.1 Mus musculus urocortin 2 (Ucn2), mRNA.

Price & Availability↑

CloneID	OMu18376
Clone ID Related Accession (Same CDS sequence)	NM_145077.1
Accession Version	NM_145077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	urocortin-2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC140383.2. On Jul 25, 2009 this sequence version replaced XM_910603.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGATGACCA GGTGGGCACT GGTGGTGTTC GTGGTCCTGA TGTTGGATAG GATCCTATTT 
GTCCCAGGAA CTCCTATCCC CACCTTCCAG CTCCTCCCTC AGAACTCTCT GGAGACAACT 
CCTAGCTCTG TGACCTCAGA GAGCTCCTCA GGTACCACCA CAGGACCCTC AGCTTCCTGG 
AGCAACTCTA AAGCCAGCCC TTACCTAGAC ACCCGTGTCA TACTCTCCCT GGATGTTCCC 
ATTGGCCTCC TACGGATCTT ACTGGAACAG GCTCGTTACA AGGCTGCCAG GAATCAGGCT 
GCCACTAATG CTCAAATACT AGCCCATGTT GGCCGCCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659543.1
CDS	10..351
Translation	MMTRWALVVFVVLMLDRILFVPGTPIPTFQLLPQNSLETTPSSVTSESSSGTTTGPSASWSNSKASPYLDTRVILSLDVPIGLLRILLEQARYKAARNQAATNAQILAHVGRR

Target ORF information:

RefSeq Version	NM_145077.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus urocortin 2 (Ucn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145077.1

ORF Insert Sequence:

ATGATGACCA GGTGGGCACT GGTGGTGTTC GTGGTCCTGA TGTTGGATAG GATCCTATTT 
GTCCCAGGAA CTCCTATCCC CACCTTCCAG CTCCTCCCTC AGAACTCTCT GGAGACAACT 
CCTAGCTCTG TGACCTCAGA GAGCTCCTCA GGTACCACCA CAGGACCCTC AGCTTCCTGG 
AGCAACTCTA AAGCCAGCCC TTACCTAGAC ACCCGTGTCA TACTCTCCCT GGATGTTCCC 
ATTGGCCTCC TACGGATCTT ACTGGAACAG GCTCGTTACA AGGCTGCCAG GAATCAGGCT 
GCCACTAATG CTCAAATACT AGCCCATGTT GGCCGCCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of gonadotropins by urocortin 2 in gonadotropic tumor L?T2 cells.
Neuroscience letters66063-67(2017 Nov)
Kageyama K,Murasawa S,Niioka K,Otsuka F,Yagi H,Daimon M

Restoration of pharyngeal dilator muscle force in dystrophin-deficient (mdx) mice following co-treatment with neutralizing interleukin-6 receptor antibodies and urocortin 2.
Experimental physiology102(9)1177-1193(2017 Sep)
Burns DP,Rowland J,Canavan L,Murphy KH,Brannock M,O'Malley D,O'Halloran KD,Edge D

Gp91phox-derived Reactive Oxygen Species/Urocortin 2/Corticotropin-releasing Hormone Receptor Type 2 Play an Important Role in Long-term Ultraviolet A Eye Irradiation-induced Photoaging.
Photochemistry and photobiology92(1)180-6(2016 Jan-Feb)
Hiramoto K,Yamate Y

Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
Endocrinology156(2)670-9(2015 Feb)
Voltolini C,Battersby S,Novembri R,Torricelli M,Severi FM,Petraglia F,Norman JE

Posttranslational processing of human and mouse urocortin 2: characterization and bioactivity of gene products.
Endocrinology154(4)1553-64(2013 Apr)
Vaughan JM,Donaldson CJ,Fischer WH,Perrin MH,Rivier JE,Sawchenko PE,Vale WW