Usp33 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp33
Entrez Gene ID	170822
Full Name	ubiquitin specific peptidase 33
Synonyms	9830169D19Rik,AA409780,Vdu1
General protein information	Preferred Names ubiquitin specific peptidase 33 Names ubiquitin carboxyl-terminal hydrolase 33 VHL-interacting deubiquitinating enzyme 1 Vhlh-interacting deubiquitinating enzyme 1 deubiquitinating enzyme 33 pVHL-interacting deubiquitinating enzyme 1 ubiquitin specific protease 33 ubiquitin thioesterase 33 ubiquitin thiolesterase 33 ubiquitin-specific-processing protease 33
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_133247.3	NP_573510.2	ubiquitin carboxyl-terminal hydrolase 33 isoform 1
NM_001076676.2	NP_001070144.1	ubiquitin carboxyl-terminal hydrolase 33 isoform 2
NM_001355666.1	NP_001342595.1	ubiquitin carboxyl-terminal hydrolase 33 isoform 4
XM_030252443.1	XP_030108303.1	ubiquitin carboxyl-terminal hydrolase 33 isoform X1
XM_030252444.1	XP_030108304.1	ubiquitin carboxyl-terminal hydrolase 33 isoform X2
XM_006501079.2	XP_006501142.1	ubiquitin carboxyl-terminal hydrolase 33 isoform X1
NM_001252486.1	NP_001239415.1	ubiquitin carboxyl-terminal hydrolase 33 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	USP33	XP_003308213.1
	LOC710693	XP_002808266.1
Xenopus tropicalis (tropical clawed frog)	usp33	NP_001016228.1
Mus musculus (house mouse)	Usp33	NP_573510.2
Homo sapiens (human)	USP33	NP_055832.3
Rattus norvegicus (Norway rat)	Usp33	NP_001178023.1
Danio rerio (zebrafish)	usp33	NP_998392.1
Bos taurus (cattle)	USP33	NP_001094609.1
Canis lupus familiaris (dog)	USP33	XP_005622106.1
Gallus gallus (chicken)	USP33	XP_004936727.1

Gene Ontology
Bibliography

Related articles in PubMed

The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination.
Cubillos-Rojas M, Schneider T, Hadjebi O, Pedrazza L, de Oliveira JR, Langa F, Gu?net JL, Duran J, de Anta JM, Alc?ntara S, Ruiz R, P?rez-Villegas EM, Aguilar-Montilla FJ, Carri?n ?M, Armengol JA, Baple E, Crosby AH, Bartrons R, Ventura F, Rosa JL
Oncotarget7(35)56083-56106(2016 Aug)

Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.
Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
The Journal of biological chemistry289(28)19789-98(2014 Jul)

Midline crossing and Slit responsiveness of commissural axons require USP33.
Yuasa-Kawada J, Kinoshita-Kawada M, Wu G, Rao Y, Wu JY
Nature neuroscience12(9)1087-9(2009 Sep)

Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration.
Yuasa-Kawada J, Kinoshita-Kawada M, Rao Y, Wu JY
Proceedings of the National Academy of Sciences of the United States of America106(34)14530-5(2009 Aug)

Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.
Li Z, Na X, Wang D, Schoen SR, Messing EM, Wu G
The Journal of biological chemistry277(7)4656-62(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown or chemical inhibition of p97 causes robust accumulation of USP33 due to inhibition of its degradation
Title: Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.

Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling
Title: Midline crossing and Slit responsiveness of commissural axons require USP33.

The following Usp33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01227	NM_001076676.2 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OMu05115	NM_001252486.1 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu09982	NM_133247.3 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OMu31674	NM_001355666.1 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu31674	XM_006501079.2 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09982	XM_030252443.1 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu88165	XM_030252444.1 Latest version!	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp33 ( NM_001076676.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001070144.1 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01227
Clone ID Related Accession (Same CDS sequence)	NM_001076676.2
Accession Version	NM_001076676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087467.1, AK082943.1 and AA210301.1. On Nov 16, 2011 this sequence version replaced NM_001076676.1. Transcript Variant: This variant (2) uses two alternate in-frame splice junctions compared to variant 1, resulting in a shorter protein (isoform 2). ##Evidence-Data-START## Transcript exon combination :: AK082251.1, AK082943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTTAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ACAACATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAGAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTCGAT 
CTTCAGCCAT TTCTTGCAAA GGACAGCCCA GCTCAGATTG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ACCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CCGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACT 
GTACAGAATG CCGAGGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAAG 
GAGAGGCGGA GGATATCAAA TTTGTTGAAC ATCATGGAAC CTAGCCTCCT TCAGTTCTAC 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGATTTTC TCTGTATCCA TGGAGGTATT CCTCCACGAA AAGCGAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ATAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCATCTCTA CATCTGCCAC ACCTGCCAAA TTGAGTTAGA GAAGATTGAA 
AAACGAAGAA AAACCGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAG 
GACTCCCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGATC CCCCAGGTCC AATCGACAAC ACTAAAATTG CGGTTACTAA ATGTGGCAGT 
GTGATGCTCA AGCAAGGAGC AGACTCTGGT CAAATTTCAG AAGAAACATG GAATTTCCTG 
CAGTCTATAT ATGGTGGGGG GCCTGAAGTT ATCCTCCGAC CTCCAGTTGT TCATGTTGAC 
CCTGATGTAC TCCAAGCAGA GGAAAAGATT GAAGTAGAAA CTCGCTCTTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070144.1
CDS	214..2907
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIELEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKDPPGPIDNTKIAVTKCGSVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDVLQAEEKIEVETRSL

Target ORF information:

RefSeq Version	NM_001076676.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076676.2

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTTAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ACAACATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAGAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTCGAT 
CTTCAGCCAT TTCTTGCAAA GGACAGCCCA GCTCAGATTG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ACCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CCGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACT 
GTACAGAATG CCGAGGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAAG 
GAGAGGCGGA GGATATCAAA TTTGTTGAAC ATCATGGAAC CTAGCCTCCT TCAGTTCTAC 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGATTTTC TCTGTATCCA TGGAGGTATT CCTCCACGAA AAGCGAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ATAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCATCTCTA CATCTGCCAC ACCTGCCAAA TTGAGTTAGA GAAGATTGAA 
AAACGAAGAA AAACCGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAG 
GACTCCCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGATC CCCCAGGTCC AATCGACAAC ACTAAAATTG CGGTTACTAA ATGTGGCAGT 
GTGATGCTCA AGCAAGGAGC AGACTCTGGT CAAATTTCAG AAGAAACATG GAATTTCCTG 
CAGTCTATAT ATGGTGGGGG GCCTGAAGTT ATCCTCCGAC CTCCAGTTGT TCATGTTGAC 
CCTGATGTAC TCCAAGCAGA GGAAAAGATT GAAGTAGAAA CTCGCTCTTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( NM_001252486.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239415.1 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu05115
Clone ID Related Accession (Same CDS sequence)	NM_001252486.1
Accession Version	NM_001252486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087467.1, AK143844.1, BC089315.1 and AA210301.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1, resulting in a shorter protein (isoform 3). ##Evidence-Data-START## Transcript exon combination :: AK087467.1, SRR1660811.179005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTTAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ACAACATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAGAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTCGAT 
CTTCAGCCAT TTCTTGCAAA GGACAGCCCA GCTCAGATTG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ACCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CCGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACT 
GTACAGAATG CCGAGGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAAG 
GAGAGGCGGA GGATATCAAA TTTGTTGAAC ATCATGGAAC CTAGCCTCCT TCAGTTCTAC 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGATTTTC TCTGTATCCA TGGAGGTATT CCTCCACGAA AAGCGAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ATAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCATCTCTA CATCTGCCAC ACCTGCCAAA TTGAGTTAGA GAAGATTGAA 
AAACGAAGAA AAACCGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAG 
GACTCCCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGGTA AGGATGGAGA TCCCCCAGGT CCAATCGACA ACACTAAAAT TGCGGTTACT 
AAATGTGGCA GTGTGATGCT CAAGCAAGGA GCAGACTCTG GTCAAATTTC AGAAGAAACA 
TGGAATTTCC TGCAGTCTAT ATATGGTGGG GGGCCTGAAG TTATCCTCCG ACCTCCAGTT 
GTTCATGTTG ACCCTGATGT ACTCCAAGCA GAGGAAAAGA TTGAAGTAGA AACTCGCTCT 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239415.1
CDS	214..2919
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIELEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGSVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDVLQAEEKIEVETRSL

Target ORF information:

RefSeq Version	NM_001252486.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252486.1

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTTAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ACAACATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAGAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTCGAT 
CTTCAGCCAT TTCTTGCAAA GGACAGCCCA GCTCAGATTG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ACCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CCGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACT 
GTACAGAATG CCGAGGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAAG 
GAGAGGCGGA GGATATCAAA TTTGTTGAAC ATCATGGAAC CTAGCCTCCT TCAGTTCTAC 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGATTTTC TCTGTATCCA TGGAGGTATT CCTCCACGAA AAGCGAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ATAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCATCTCTA CATCTGCCAC ACCTGCCAAA TTGAGTTAGA GAAGATTGAA 
AAACGAAGAA AAACCGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAG 
GACTCCCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGGTA AGGATGGAGA TCCCCCAGGT CCAATCGACA ACACTAAAAT TGCGGTTACT 
AAATGTGGCA GTGTGATGCT CAAGCAAGGA GCAGACTCTG GTCAAATTTC AGAAGAAACA 
TGGAATTTCC TGCAGTCTAT ATATGGTGGG GGGCCTGAAG TTATCCTCCG ACCTCCAGTT 
GTTCATGTTG ACCCTGATGT ACTCCAAGCA GAGGAAAAGA TTGAAGTAGA AACTCGCTCT 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( NM_133247.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_573510.2 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 1, mRNA.

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CloneID	OMu09982
Clone ID Related Accession (Same CDS sequence)	NM_133247.3 , XM_030252443.1
Accession Version	NM_133247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087467.1, AK143844.1, BC089315.1 and AA210301.1. On Nov 16, 2011 this sequence version replaced NM_133247.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF383174.1, SRR1660817.16866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GGTATGGAGG AGGGCCTGCT GTCAACCATC TCTACATCTG CCACACCTGC 
CAAATTGAGT TAGAGAAGAT TGAAAAACGA AGAAAAACCG AATTGGAAAT TTTTATTCGG 
CTCAACAGAG CATTTCAAGA GGAGGACTCC CCAGCTACTT TTTACTGTAT CAGCATGCAG 
TGGTTTAGAG AATGGGAGAG TTTTGTAAAG GGTAAGGATG GAGATCCCCC AGGTCCAATC 
GACAACACTA AAATTGCGGT TACTAAATGT GGCAGTGTGA TGCTCAAGCA AGGAGCAGAC 
TCTGGTCAAA TTTCAGAAGA AACATGGAAT TTCCTGCAGT CTATATATGG TGGGGGGCCT 
GAAGTTATCC TCCGACCTCC AGTTGTTCAT GTTGACCCTG ATGTACTCCA AGCAGAGGAA 
AAGATTGAAG TAGAAACTCG CTCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573510.2
CDS	214..2943
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIELEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGSVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDVLQAEEKIEVETRSL

Target ORF information:

RefSeq Version	NM_133247.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133247.3

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GGTATGGAGG AGGGCCTGCT GTCAACCATC TCTACATCTG CCACACCTGC 
CAAATTGAGT TAGAGAAGAT TGAAAAACGA AGAAAAACCG AATTGGAAAT TTTTATTCGG 
CTCAACAGAG CATTTCAAGA GGAGGACTCC CCAGCTACTT TTTACTGTAT CAGCATGCAG 
TGGTTTAGAG AATGGGAGAG TTTTGTAAAG GGTAAGGATG GAGATCCCCC AGGTCCAATC 
GACAACACTA AAATTGCGGT TACTAAATGT GGCAGTGTGA TGCTCAAGCA AGGAGCAGAC 
TCTGGTCAAA TTTCAGAAGA AACATGGAAT TTCCTGCAGT CTATATATGG TGGGGGGCCT 
GAAGTTATCC TCCGACCTCC AGTTGTTCAT GTTGACCCTG ATGTACTCCA AGCAGAGGAA 
AAGATTGAAG TAGAAACTCG CTCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( NM_001355666.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342595.1 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu31674
Clone ID Related Accession (Same CDS sequence)	NM_001355666.1 , XM_006501079.2
Accession Version	NM_001355666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122529.5 and AA210301.1. On Sep 23, 2017 this sequence version replaced XM_006501079.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.8945.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GATCCCCCAG GTCCAATCGA CAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342595.1
CDS	214..2532
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRSPRSNRQH

Target ORF information:

RefSeq Version	NM_001355666.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355666.1

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GATCCCCCAG GTCCAATCGA CAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( XM_006501079.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501142.1 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31674
Clone ID Related Accession (Same CDS sequence)	NM_001355666.1 , XM_006501079.2
Accession Version	XM_006501079.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GATCCCCCAG GTCCAATCGA CAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501142.1
CDS	208..2526
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRSPRSNRQH

Target ORF information:

RefSeq Version	XM_006501079.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501079.2

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GATCCCCCAG GTCCAATCGA CAACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( XM_030252443.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108303.1 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09982
Clone ID Related Accession (Same CDS sequence)	NM_133247.3 , XM_030252443.1
Accession Version	XM_030252443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GGTATGGAGG AGGGCCTGCT GTCAACCATC TCTACATCTG CCACACCTGC 
CAAATTGAGT TAGAGAAGAT TGAAAAACGA AGAAAAACCG AATTGGAAAT TTTTATTCGG 
CTCAACAGAG CATTTCAAGA GGAGGACTCC CCAGCTACTT TTTACTGTAT CAGCATGCAG 
TGGTTTAGAG AATGGGAGAG TTTTGTAAAG GGTAAGGATG GAGATCCCCC AGGTCCAATC 
GACAACACTA AAATTGCGGT TACTAAATGT GGCAGTGTGA TGCTCAAGCA AGGAGCAGAC 
TCTGGTCAAA TTTCAGAAGA AACATGGAAT TTCCTGCAGT CTATATATGG TGGGGGGCCT 
GAAGTTATCC TCCGACCTCC AGTTGTTCAT GTTGACCCTG ATGTACTCCA AGCAGAGGAA 
AAGATTGAAG TAGAAACTCG CTCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108303.1
CDS	293..3022
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIELEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGSVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDVLQAEEKIEVETRSL

Target ORF information:

RefSeq Version	XM_030252443.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252443.1

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TTACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGACAACA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAGA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT CGATCTTCAG CCATTTCTTG CAAAGGACAG CCCAGCTCAG 
ATTGTGACAT ATGATCTCCT GTCAGTCATC TGTCACCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCCGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACTGTACAG AATGCCGAGG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAAGGAGAGG CGGAGGATAT CAAATTTGTT GAACATCATG 
GAACCTAGCC TCCTTCAGTT CTACATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAT TTTCTCTGTA TCCATGGAGG TATTCCTCCA 
CGAAAAGCGA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTATAGCA GGTATGGAGG AGGGCCTGCT GTCAACCATC TCTACATCTG CCACACCTGC 
CAAATTGAGT TAGAGAAGAT TGAAAAACGA AGAAAAACCG AATTGGAAAT TTTTATTCGG 
CTCAACAGAG CATTTCAAGA GGAGGACTCC CCAGCTACTT TTTACTGTAT CAGCATGCAG 
TGGTTTAGAG AATGGGAGAG TTTTGTAAAG GGTAAGGATG GAGATCCCCC AGGTCCAATC 
GACAACACTA AAATTGCGGT TACTAAATGT GGCAGTGTGA TGCTCAAGCA AGGAGCAGAC 
TCTGGTCAAA TTTCAGAAGA AACATGGAAT TTCCTGCAGT CTATATATGG TGGGGGGCCT 
GAAGTTATCC TCCGACCTCC AGTTGTTCAT GTTGACCCTG ATGTACTCCA AGCAGAGGAA 
AAGATTGAAG TAGAAACTCG CTCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( XM_030252444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108304.1 Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X2, mRNA.

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CloneID	OMu88165
Clone ID Related Accession (Same CDS sequence)	XM_030252444.1
Accession Version	XM_030252444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTTAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ACAACATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAGAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTCGAT 
CTTCAGCCAT TTCTTGCAAA GGACAGCCCA GCTCAGATTG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ACCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CCGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACT 
GTACAGAATG CCGAGGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAAG 
GAGAGGCGGA GGATATCAAA TTTGTTGAAC ATCATGGAAC CTAGCCTCCT TCAGTTCTAC 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGATTTTC TCTGTATCCA TGGAGGTATT CCTCCACGAA AAGCGAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ATAGCAGATC CCCCAGGTCC 
AATCGACAAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108304.1
CDS	508..2802
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEKSQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELDKDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSPPHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRSPRSNRQH

Target ORF information:

RefSeq Version	XM_030252444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 33 (Usp33), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252444.1

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACGAAAGAG 
GACTTGATAC AGAAATCTCT CGGCGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCGTGCC TGGAGAATAG GTGCTCCTAT GTTGGCTGCG GGGAGTCGCA AGTGGACCAC 
AGCACCATAC ACTCGCAGGA GACAAAGCAT TATCTAACTG TGAACCTCAC CACTCTTCGA 
GTATGGTGTT ATGCTTGCAG CAAAGAAGTA TTTTTGGATA GAAAATTAGG AACTCCTCCT 
TCATTACCTC ATGTAAGACA GCCTCAACAA ACACAAGAAA ACAGTGTCCA GGATTTTAAA 
ATTCCCAGTA ATCCAGCATT GAAAACCCCC ATGGTTGCTG TGTCTGAAGA TCTGGATATA 
GAAGTGGAAG AGGAAGACGA GCTGAAGGCT AGAGGCCTGA CAGGTTTGAA AAACATTGGA 
AATACTTGTT ATATGAACGC GGCGCTGCAG GCTCTTTCTA ACTGCCCACC TTTGACACAG 
TTCTTTCTTG ATTGTGGAGG ACTGGCTAGA ACAGATAAGA AACCAGCAAT TTGTAAAAGT 
TATCTCAAAC TAATGACCGA GTTGTGGCAC AAGAGCAGGC CAGGATCTGT TGTGCCGGCT 
AATCTGTTCC AAGGAATTAA AACTGTAAAT CCAACTTTTC GAGGTTATTC TCAGCAGGAT 
GCCCAGGAAT TCCTTCGCTG TCTAATGGAC CTGCTTCATG AGGAGCTGAA GGAGCAGGTC 
ATGGAAATGG AGGAAGAGCC TCAAACACTA ACTTCTGAGG AGACGGTGGA GGAAGAGAAG 
AGCCAGTCAG ATGTGGATTT TCAGTCGTGC GAGTCTTGTA GCAGCAGCGA GAAAGCAGAA 
AATGAGAGTG GCTCCAAAGG CTTTCCTGAG GACAGCAATG AGACCACCAT GCTCATCCAG 
GACGAGGATG ACCTGGAGAT GGCCAAAGAC TGGCAGAAAG AGAAGGTGTG CAATAAGATC 
AACAAGGCAA ATGCCGATGT AGAACTGGAC AAAGACAGGG ACACAGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAAGTG CAGATACACG GCAGAGCCTC AGAATCTATC 
ACTGATGTCC ATCTGAATGA CCTAGCTACG TCACAGATCC TTCCTTCAAA TGAAAGTGTT 
AGTCCACGGT TATCAGCAAG CCCTCCTAAG CTAGGCAGTC TGTGGCCAGG ACTGTCGCCT 
CCACACAAGA AAGCTCAGTC TACATCTGCA AAGAGGAAAA AGCAGCATAA GAAATACAGA 
AGTGTCATCT CCGACATATT CGATGGAACA GTCATTAGCT CGGTACAGTG TCTGACGTGC 
GATAGGGTGT CTATAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAGGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCATGT 
GGTGAAGCGT ACGCCCCGCA GGGGTGGATA GCTTTCTTCA TGGAGTATGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTTAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ACAACATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAGAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTCGAT 
CTTCAGCCAT TTCTTGCAAA GGACAGCCCA GCTCAGATTG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ACCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CCGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACT 
GTACAGAATG CCGAGGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAAG 
GAGAGGCGGA GGATATCAAA TTTGTTGAAC ATCATGGAAC CTAGCCTCCT TCAGTTCTAC 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGATTTTC TCTGTATCCA TGGAGGTATT CCTCCACGAA AAGCGAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ATAGCAGATC CCCCAGGTCC 
AATCGACAAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination.
Oncotarget7(35)56083-56106(2016 Aug)
Cubillos-Rojas M,Schneider T,Hadjebi O,Pedrazza L,de Oliveira JR,Langa F,Gu?net JL,Duran J,de Anta JM,Alc?ntara S,Ruiz R,P?rez-Villegas EM,Aguilar-Montilla FJ,Carri?n ?M,Armengol JA,Baple E,Crosby AH,Bartrons R,Ventura F,Rosa JL

Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.
The Journal of biological chemistry289(28)19789-98(2014 Jul)
Chan NC,den Besten W,Sweredoski MJ,Hess S,Deshaies RJ,Chan DC

Midline crossing and Slit responsiveness of commissural axons require USP33.
Nature neuroscience12(9)1087-9(2009 Sep)
Yuasa-Kawada J,Kinoshita-Kawada M,Wu G,Rao Y,Wu JY

Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration.
Proceedings of the National Academy of Sciences of the United States of America106(34)14530-5(2009 Aug)
Yuasa-Kawada J,Kinoshita-Kawada M,Rao Y,Wu JY

Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.
The Journal of biological chemistry277(7)4656-62(2002 Feb)
Li Z,Na X,Wang D,Schoen SR,Messing EM,Wu G

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Usp33 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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