Capn8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capn8
Entrez Gene ID	170725
Full Name	calpain 8
Synonyms	nCL-2,nCL-2'
General protein information	Preferred Names calpain 8 Names calpain-8 new calpain 2 stomach-specific M-type calpain stomach-specific calpain (nCL-2)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H5

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496686.2	XP_006496749.1	calpain-8 isoform X2
XM_006496688.3	XP_006496751.1	calpain-8 isoform X3
XM_017319052.1	XP_017174541.1	calpain-8 isoform X1
NM_001145806.1	NP_001139278.1	calpain-8 isoform 2
NM_130890.2	NP_570960.2	calpain-8 isoform 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CAPN8	XP_426117.2
Caenorhabditis elegans (roundworm)	clp-4	NP_497460.2
Caenorhabditis elegans (roundworm)	clp-3	NP_493052.2
Caenorhabditis elegans (roundworm)	clp-7	NP_500082.1
Caenorhabditis elegans (roundworm)	clp-1	NP_498740.2
Mus musculus (house mouse)	Capn8	NP_570960.2
Homo sapiens (human)	CAPN8	NP_001137434.1
Canis lupus familiaris (dog)	CAPN8	XP_854850.3
Caenorhabditis elegans (roundworm)	clp-6	NP_500081.1
Xenopus tropicalis (tropical clawed frog)	capn8	NP_001039108.1
Xenopus tropicalis (tropical clawed frog)	LOC100497284	XP_004914783.1
Rattus norvegicus (Norway rat)	Capn8	NP_579843.2
Danio rerio (zebrafish)	capn8	NP_001231009.1

Gene Ontology
Bibliography

Related articles in PubMed

A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively.
Hata S, Kitamura F, Yamaguchi M, Shitara H, Murakami M, Sorimachi H
The Journal of biological chemistry291(53)27313-27322(2016 12)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.
Hata S, Abe M, Suzuki H, Kitamura F, Toyama-Sorimachi N, Abe K, Sakimura K, Sorimachi H
PLoS genetics6(7)e1001040(2010 Jul)

Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humans.
Harrill AH, Watkins PB, Su S, Ross PK, Harbourt DE, Stylianou IM, Boorman GA, Russo MW, Sackler RS, Harris SC, Smith PC, Tennant R, Bogue M, Paigen K, Harris C, Contractor T, Wiltshire T, Rusyn I, Threadgill DW
Genome research19(9)1507-15(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gastrointestinal-tract-specific calpains 8 and 9 formed a complex we termed "G-calpain," in which both were essential for gastric mucosal defense
Title: Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.

nCL-2/calpain 8 is active without calpain regulatory subunit and oligomerizes through C2-like domains
Title: Stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains.

nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP
Title: Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP.

The following Capn8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06949	NM_001145806.1 Latest version!	Mus musculus calpain 8 (Capn8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu28647	XM_006496688.3 Latest version!	Mus musculus calpain 8 (Capn8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu09849	NM_130890.2 Latest version!	Mus musculus calpain 8 (Capn8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$412.30 $589.00
OMu28645	XM_006496686.2 Latest version!	Mus musculus calpain 8 (Capn8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu77008	XM_017319052.1 Latest version!	Mus musculus calpain 8 (Capn8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capn8 ( NM_001145806.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139278.1 Mus musculus calpain 8 (Capn8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06949
Clone ID Related Accession (Same CDS sequence)	NM_001145806.1
Accession Version	NM_001145806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Mus musculus(house mouse)
Product	calpain-8 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132449.1 and AB050203.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK132449.1, AB050203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGGCTCC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139278.1
CDS	55..1200
Translation	MAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPGSF

Target ORF information:

RefSeq Version	NM_001145806.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 8 (Capn8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145806.1

ORF Insert Sequence:

ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGGCTCC 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn8 ( XM_006496688.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496751.1 Mus musculus calpain 8 (Capn8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu28647
Clone ID Related Accession (Same CDS sequence)	XM_006496688.3
Accession Version	XM_006496688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpain-8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA GGACACAGAA GCCCTGCTTT CCTGTCACCT ACAGGACGCA CAGGCAGGCC 
ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTCCACA TCCCCGTGAC ATGGATGGAG 
AAGATGAGCA CTTCTGGAGC CTGTCTGAGG AGTTTGCGGA CAAGGATTCT GAAATCAGTG 
CTCACCAGCT CAAGAGGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496751.1
CDS	250..1953
Translation	MSERTQKPCFPVTYRTHRQAMAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQIPKELENHTDEHLGRDFFQGRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCLRVFSEKKAQALHIPVTWMEKMSTSGACLRSLRTRILKSVLTSSRGS

Target ORF information:

RefSeq Version	XM_006496688.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 8 (Capn8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496688.3

ORF Insert Sequence:

ATGTCTGAGA GGACACAGAA GCCCTGCTTT CCTGTCACCT ACAGGACGCA CAGGCAGGCC 
ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTCCACA TCCCCGTGAC ATGGATGGAG 
AAGATGAGCA CTTCTGGAGC CTGTCTGAGG AGTTTGCGGA CAAGGATTCT GAAATCAGTG 
CTCACCAGCT CAAGAGGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn8 ( NM_130890.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_570960.2 Mus musculus calpain 8 (Capn8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09849
Clone ID Related Accession (Same CDS sequence)	NM_130890.2
Accession Version	NM_130890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	calpain-8 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132449.1, BC125504.1, AB050202.1 and AC123793.5. On Mar 11, 2009 this sequence version replaced NM_130890.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB050202.1, BC125504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTAGAAA TTGGGGACGC TGTTGCTGGA 
GACCCACATG AGCCACATCC CCGTGACATG GATGGAGAAG ATGAGCACTT CTGGAGCCTG 
TCTGAGGAGT TTGCGGACAA GGATTCTGAA ATCAGTGCTC ACCAGCTCAA GAGGGTCCTG 
AATGGATTGC TTTCTAAACG AACAGACATG AAATTTGACG GATTCAACAT CAACACTTGC 
AGAGAAATGA TCAGCCTGCT GGATGGTGAT GGGACAGGAA GCCTGCGACC GGTGGAGTTC 
AAGACTCTCT GGCTGAAGAT CTGCAAGTAT CTGGAGATCT ACCAAGAAAT GGACCACAGC 
CGTGCAGGGA CCATTGATGC TCACGAGATG AGGACAGCTC TCAAGAAAGC AGGTTTCACC 
CTCAACAACC AGGTGCAGCA GACCATTGCC ACGAGGTATG CATGCAGCAA GCTTGGTGTT 
GACTTTGACG GCTTTGTGGC TTGTATGATC CGCCTGGAGA TCCTGTTCAA ATTGTTCAGA 
CTCTTGGATA AGGACCAGAA TGGCATTGTA CAGCTCTCCC TGGCTGAGTG GCTGTGCTGT 
GCACTGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570960.2
CDS	55..2166
Translation	MAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQIPKELENHTDEHLGRDFFQGRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCLRVFSEKKAQALEIGDAVAGDPHEPHPRDMDGEDEHFWSLSEEFADKDSEISAHQLKRVLNGLLSKRTDMKFDGFNINTCREMISLLDGDGTGSLRPVEFKTLWLKICKYLEIYQEMDHSRAGTIDAHEMRTALKKAGFTLNNQVQQTIATRYACSKLGVDFDGFVACMIRLEILFKLFRLLDKDQNGIVQLSLAEWLCCALV

Target ORF information:

RefSeq Version	NM_130890.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 8 (Capn8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130890.2

ORF Insert Sequence:

ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTAGAAA TTGGGGACGC TGTTGCTGGA 
GACCCACATG AGCCACATCC CCGTGACATG GATGGAGAAG ATGAGCACTT CTGGAGCCTG 
TCTGAGGAGT TTGCGGACAA GGATTCTGAA ATCAGTGCTC ACCAGCTCAA GAGGGTCCTG 
AATGGATTGC TTTCTAAACG AACAGACATG AAATTTGACG GATTCAACAT CAACACTTGC 
AGAGAAATGA TCAGCCTGCT GGATGGTGAT GGGACAGGAA GCCTGCGACC GGTGGAGTTC 
AAGACTCTCT GGCTGAAGAT CTGCAAGTAT CTGGAGATCT ACCAAGAAAT GGACCACAGC 
CGTGCAGGGA CCATTGATGC TCACGAGATG AGGACAGCTC TCAAGAAAGC AGGTTTCACC 
CTCAACAACC AGGTGCAGCA GACCATTGCC ACGAGGTATG CATGCAGCAA GCTTGGTGTT 
GACTTTGACG GCTTTGTGGC TTGTATGATC CGCCTGGAGA TCCTGTTCAA ATTGTTCAGA 
CTCTTGGATA AGGACCAGAA TGGCATTGTA CAGCTCTCCC TGGCTGAGTG GCTGTGCTGT 
GCACTGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn8 ( XM_006496686.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496749.1 Mus musculus calpain 8 (Capn8), transcript variant X3, mRNA.

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CloneID	OMu28645
Clone ID Related Accession (Same CDS sequence)	XM_006496686.2
Accession Version	XM_006496686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpain-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA GGACACAGAA GCCCTGCTTT CCTGTCACCT ACAGGACGCA CAGGCAGGCC 
ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTAGAAA TTGGGGACGC TGTTGCTGGA 
GACCCACATG AGCCACATCC CCGTGACATG GATGGAGAAG ATGAGCACTT CTGGAGCCTG 
TCTGAGGAGT TTGCGGACAA GGATTCTGAA ATCAGTGCTC ACCAGCTCAA GAGGGTCCTG 
AATGGATTGC TTTCTAAACG AACAGACATG AAATTTGACG GATTCAACAT CAACACTTGC 
AGAGAAATGA TCAGCCTGCT GGATGGTGAT GGGACAGGAA GCCTGCGACC GGTGGAGTTC 
AAGACTCTCT GGCTGAAGAT CTGCAAGTAT CTGGAGATCT ACCAAGAAAT GGACCACAGC 
CGTGCAGGGA CCATTGATGC TCACGAGATG AGGACAGCTC TCAAGAAAGC AGGTTTCACC 
CTCAACAACC AGGTGCAGCA GACCATTGCC ACGAGAATTG TTCAGACTCT TGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496749.1
CDS	251..2287
Translation	MSERTQKPCFPVTYRTHRQAMAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQIPKELENHTDEHLGRDFFQGRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCLRVFSEKKAQALEIGDAVAGDPHEPHPRDMDGEDEHFWSLSEEFADKDSEISAHQLKRVLNGLLSKRTDMKFDGFNINTCREMISLLDGDGTGSLRPVEFKTLWLKICKYLEIYQEMDHSRAGTIDAHEMRTALKKAGFTLNNQVQQTIATRIVQTLG

Target ORF information:

RefSeq Version	XM_006496686.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 8 (Capn8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496686.2

ORF Insert Sequence:

ATGTCTGAGA GGACACAGAA GCCCTGCTTT CCTGTCACCT ACAGGACGCA CAGGCAGGCC 
ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTAGAAA TTGGGGACGC TGTTGCTGGA 
GACCCACATG AGCCACATCC CCGTGACATG GATGGAGAAG ATGAGCACTT CTGGAGCCTG 
TCTGAGGAGT TTGCGGACAA GGATTCTGAA ATCAGTGCTC ACCAGCTCAA GAGGGTCCTG 
AATGGATTGC TTTCTAAACG AACAGACATG AAATTTGACG GATTCAACAT CAACACTTGC 
AGAGAAATGA TCAGCCTGCT GGATGGTGAT GGGACAGGAA GCCTGCGACC GGTGGAGTTC 
AAGACTCTCT GGCTGAAGAT CTGCAAGTAT CTGGAGATCT ACCAAGAAAT GGACCACAGC 
CGTGCAGGGA CCATTGATGC TCACGAGATG AGGACAGCTC TCAAGAAAGC AGGTTTCACC 
CTCAACAACC AGGTGCAGCA GACCATTGCC ACGAGAATTG TTCAGACTCT TGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn8 ( XM_017319052.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174541.1 Mus musculus calpain 8 (Capn8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77008
Clone ID Related Accession (Same CDS sequence)	XM_017319052.1
Accession Version	XM_017319052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calpain-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA GGACACAGAA GCCCTGCTTT CCTGTCACCT ACAGGACGCA CAGGCAGGCC 
ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTAGAAA TTGGGGACGC TGTTGCTGGA 
GACCCACATG AGCCACATCC CCGTGACATG GATGGAGAAG ATGAGCACTT CTGGAGCCTG 
TCTGAGGAGT TTGCGGACAA GGATTCTGAA ATCAGTGCTC ACCAGCTCAA GAGGGTCCTG 
AATGGATTGC TTTCTAAACG AACAGACATG AAATTTGACG GATTCAACAT CAACACTTGC 
AGAGAAATGA TCAGCCTGCT GGATGGTGAT GGGACAGGAA GCCTGCGACC GGTGGAGTTC 
AAGACTCTCT GGCTGAAGAT CTGCAAGTAT CTGGAGATCT ACCAAGAAAT GGACCACAGC 
CGTGCAGGGA CCATTGATGC TCACGAGATG AGGACAGCTC TCAAGAAAGC AGGTTTCCAC 
AGAATCATGG ATGGCCCCTG TGGTGCCATC CCCTTGAAGT CTATCAGGAA AATGCGAAGA 
CCTTCGCACC ACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174541.1
CDS	253..2310
Translation	MSERTQKPCFPVTYRTHRQAMAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQIPKELENHTDEHLGRDFFQGRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCLRVFSEKKAQALEIGDAVAGDPHEPHPRDMDGEDEHFWSLSEEFADKDSEISAHQLKRVLNGLLSKRTDMKFDGFNINTCREMISLLDGDGTGSLRPVEFKTLWLKICKYLEIYQEMDHSRAGTIDAHEMRTALKKAGFHRIMDGPCGAIPLKSIRKMRRPSHHP

Target ORF information:

RefSeq Version	XM_017319052.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calpain 8 (Capn8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319052.1

ORF Insert Sequence:

ATGTCTGAGA GGACACAGAA GCCCTGCTTT CCTGTCACCT ACAGGACGCA CAGGCAGGCC 
ATGGCAGCCC TGGCAGCTGG AATATCCAAG CAACGTGCAG CTGCTCAGGG CCTTGGCTCT 
AACCAGAATG CTGTGAAGTA TCTGGGCCAG GACTTCGAGA CCCTGAGAAA GCAATGCTTG 
AACTCAGGGG TCCTATTTAA AGACCCGGAA TTCCCGGCAT GTCCGTCAGC TTTGGGCTAC 
AGGGATCTTG GACCAGGCTC TGCAGAAACT CAAGGCATCA TATGGAAGCG ACCCACGGAA 
TTGTGTTCCA ACCCTCAGTT TATCGTTGGT GGAGCCACAC GCACAGACAT CCGCCAGGGG 
GGTCTTGGAG ACTGTTGGCT TCTGGCAGCC ATTGCCTCCC TCACCTTGAA TGAAAAGCTG 
CTTTATCGAG TGGTTCCTAG AGACCAGAGC TTCCAGAAGA ACTATGCTGG CATTTTTCAT 
TTCCAGTTCT GGCAGTATGG AGAGTGGGTG GAGGTGGTCA TTGATGACCG GCTCCCCACC 
AAGAATGGAC AGCTGCTCTT CCTACACTCC GAAGAAGGCA ATGAGTTCTG GAGTGCTCTG 
CTGGAGAAAG CCTATGCCAA GCTCAATGGT TCGTATGAGG CTCTTGCTGG AGGCTCCACA 
ATTGAGGGGT TTGAGGATTT CACGGGTGGC ATCTCTGAGT TTTATGACTT GAGGAAGCCT 
CCGGGCAATC TGTACTACAC CATCCAGAAG GCCCTGCGCA AAGGCTCTCT GCTGGGCTGC 
TCCATTGATG TCTCAAACGC AGCCGAAGCA GAAGCCACCA CCAGGCAAAA GCTGGTTAAG 
GGCCACGCAT ACTCTGTGAC TGGAGTCGAA GAGGTGGATT TCCGTGGCCT CCCAGAGAAG 
CTGATCAGGT TGAGGAACCC GTGGGGTGAA GTGGAGTGGA CGGGAGCCTG GAGTGATAGT 
GCACCTGAGT GGAATTACAT AGATCCACAG AAGAAGGGAG AACTGGACAA GAGAGCAGAA 
GATGGGGAGT TCTGGATGTC CTTTTCGGAT TTCTTGAAGC AGTTCTCCCG ACTAGAGATA 
TGCAACCTGT CCCCAGACTC TCTGAGCAGT GAGGAGATAC ACAAATGGAA CCTGGTACTC 
TTCAACGGCC GCTGGACACG GGGTTCTACA GCCGGGGGCT GCCAGAACTA CCCAGCCACT 
TACTGGACCA ACCCCCAGTT TAAAATCCAT TTGGACGAGG TGGATGAGGA CCAGGAGGAG 
GGCACCAGTG AACCCTGCTG TACAGTGCTG CTGGGTCTGA TGCAGAAGAA TCGCAGACGT 
CAGAGGAGGA TCGGCCAGGG CATGCTCAGC ATAGGCTATG CTGTCTACCA GATTCCCAAG 
GAGCTGGAGA ATCACACCGA TGAGCACCTG GGCCGGGATT TCTTCCAGGG CCGCCAGCCC 
TCCACCTGCT CCAGCACCTA CATGAACCTT CGAGAGGTGT CCAGCAGGGT CCAACTCCCC 
CCAGGACAGT ACCTGGTGGT GCCATCCACC TTTGAGCCCT TCAAGGACGG TGACTTCTGC 
CTGAGGGTGT TCTCGGAGAA GAAGGCCCAG GCTCTAGAAA TTGGGGACGC TGTTGCTGGA 
GACCCACATG AGCCACATCC CCGTGACATG GATGGAGAAG ATGAGCACTT CTGGAGCCTG 
TCTGAGGAGT TTGCGGACAA GGATTCTGAA ATCAGTGCTC ACCAGCTCAA GAGGGTCCTG 
AATGGATTGC TTTCTAAACG AACAGACATG AAATTTGACG GATTCAACAT CAACACTTGC 
AGAGAAATGA TCAGCCTGCT GGATGGTGAT GGGACAGGAA GCCTGCGACC GGTGGAGTTC 
AAGACTCTCT GGCTGAAGAT CTGCAAGTAT CTGGAGATCT ACCAAGAAAT GGACCACAGC 
CGTGCAGGGA CCATTGATGC TCACGAGATG AGGACAGCTC TCAAGAAAGC AGGTTTCCAC 
AGAATCATGG ATGGCCCCTG TGGTGCCATC CCCTTGAAGT CTATCAGGAA AATGCGAAGA 
CCTTCGCACC ACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively.
The Journal of biological chemistry291(53)27313-27322(2016 12)
Hata S,Kitamura F,Yamaguchi M,Shitara H,Murakami M,Sorimachi H

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.
PLoS genetics6(7)e1001040(2010 Jul)
Hata S,Abe M,Suzuki H,Kitamura F,Toyama-Sorimachi N,Abe K,Sakimura K,Sorimachi H

Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humans.
Genome research19(9)1507-15(2009 Sep)
Harrill AH,Watkins PB,Su S,Ross PK,Harbourt DE,Stylianou IM,Boorman GA,Russo MW,Sackler RS,Harris SC,Smith PC,Tennant R,Bogue M,Paigen K,Harris C,Contractor T,Wiltshire T,Rusyn I,Threadgill DW

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Capn8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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