Lig1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lig1
Entrez Gene ID	16881
Full Name	ligase I, DNA, ATP-dependent
Synonyms	AL033288,LigI
General protein information	Preferred Names ligase I, DNA, ATP-dependent Names DNA ligase 1 DNA ligase I polydeoxyribonucleotide synthase [ATP] 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A1\|7 7.82 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199310.1	NP_001186239.1	DNA ligase 1
NM_001083188.1	NP_001076657.1	DNA ligase 1
NM_010715.3	NP_034845.3	DNA ligase 1
NM_010715.2	NP_034845.2	DNA ligase 1
NM_001199310.2	NP_001186239.2	DNA ligase 1
NM_001083188.2	NP_001076657.2	DNA ligase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LIG1	NP_000225.1
Danio rerio (zebrafish)	si:dkeyp-35b8.5	NP_001119860.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009222	XP_319999.3
Saccharomyces cerevisiae (baker's yeast)	CDC9	NP_010117.1
Arabidopsis thaliana (thale cress)	AT1G49250	NP_175351.1
	LIG1	XP_002801366.1
Rattus norvegicus (Norway rat)	Lig1	NP_001019439.1
Gallus gallus (chicken)	LIG1	XP_003643178.2
Drosophila melanogaster (fruit fly)	DNA-ligI	NP_611843.2
Caenorhabditis elegans (roundworm)	lig-1	NP_741625.2
Eremothecium gossypii	AGOS_ACL155W	NP_983249.1
Oryza sativa (rice)	Os10g0489200	NP_001064917.1
Xenopus tropicalis (tropical clawed frog)	lig1	XP_002941929.1
Bos taurus (cattle)	LIG1	NP_001096018.1
Kluyveromyces lactis	KLLA0D12496g	XP_453619.1
Mus musculus (house mouse)	Lig1	NP_001076657.1
Magnaporthe oryzae (rice blast fungus)	MGG_06370	XP_003717204.1
Arabidopsis thaliana (thale cress)	LIG1	NP_172293.2
Pan troglodytes (chimpanzee)	LIG1	XP_001170212.3
Canis lupus familiaris (dog)	LIG1	XP_005616432.1
Rattus norvegicus (Norway rat)	LOC100911727	XP_003748816.2
Schizosaccharomyces pombe (fission yeast)	cdc17	NP_593318.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
Zhou L, Dai H, Wu J, Zhou M, Yuan H, Du J, Yang L, Wu X, Xu H, Hua Y, Xu J, Zheng L, Shen B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)

LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.
Boillot M, Lee CY, Allene C, Leguern E, Baulac S, Rouach N
Scientific reports621769(2016 Feb)

DNA ligase I is not essential for mammalian cell viability.
Han L, Masani S, Hsieh CL, Yu K
Cell reports7(2)316-20(2014 Apr)

Genome-wide ENU mutagenesis in combination with high density SNP analysis and exome sequencing provides rapid identification of novel mouse models of developmental disease.
Caruana G, Farlie PG, Hart AH, Bagheri-Fam S, Wallace MJ, Dobbie MS, Gordon CT, Miller KA, Whittle B, Abud HE, Arkell RM, Cole TJ, Harley VR, Smyth IM, Bertram JF
PloS one8(3)e55429(2013)

The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos.
Linke M, May A, Reifenberg K, Haaf T, Zechner U
Cytogenetic and genome research139(2)71-9(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased presynaptic glutamate release is a key early event resulting from Lgi1-deficiency, which likely contributes to epileptogenesis.
Title: LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.

Lig1 is not absolutely required for cellular DNA replication and repair in Lig1-null cell line.
Title: DNA ligase I is not essential for mammalian cell viability.

LigI has a highly specific role in CTG repeat maintenance in the maternal germline, involved in mediating CTG expansions and in the avoidance of maternal CTG contractions.
Title: Maternal germline-specific effect of DNA ligase I on CTG/CAG instability.

DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability.
Title: DNA ligase I null mouse cells show normal DNA repair activity but altered DNA replication and reduced genome stability.

The following Lig1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lig1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02217	NM_001083188.1 Latest version!	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu02217	NM_001199310.1 Latest version!	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu02217	NM_010715.2 Latest version!	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu90644	NM_010715.3 Latest version!	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu90644	NM_001199310.2 Latest version!	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu90644	NM_001083188.2 Latest version!	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lig1 ( NM_001199310.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186239.1 Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu02217
Clone ID Related Accession (Same CDS sequence)	NM_001199310.1 , NM_001083188.1 , NM_010715.2
Accession Version	NM_001199310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Mus musculus(house mouse)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC138541.1 and BE996000.1. Transcript Variant: This variant (3) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138541.1, U19604.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGAAAAA AAGAGCAAGA GAGGAAAGGA GAGACTTCTG CTGCCAACAT GCAGAGAAGT 
ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA AGAAGCCAGA GAAGGAGACA 
CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG CGCTGAAGGA GAGGAATCAA 
GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA GGAAGGTAGC CCAGGTTCTG 
AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC CCAAAGTCCA GAAGCCTGTG 
TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT GTCCTGAGAA CAGTCCTGTC 
TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG TCGAACTGCG 
CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG AGGAGCAGAA TGAGGACAAA 
ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC CAAAAGAAAG CCTCGCAGAG 
GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC AGCTCATAGT CCCCTCTGAG 
CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG AGAACATTCC AGTGTGTAAG 
GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA AACCTCCTGC TAGAGGTGCC 
AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG TGAAAACTGA AGTCAAACAA 
GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA CCCTGGATCC AGCAAATTAT 
AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT GCTGGAAACA TGGCCAGAAG 
GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG AGGAGGTTTC TGCTAGGCTC 
AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC CCCTCCAGAC 
TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC CACCTCAGCA GGGACTAGAG 
CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC AGGCCACAGG TCGTCAGCTG 
GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG GGCTGGTGGC CGAGAACAGC 
CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA CTATTTCTGG AGTCTTCACC 
AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA TGGCCAAGAA GATGGACATT 
ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA GGTACATTGC CAGGTCCCTA 
AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC TGGCTGCTCT TGCCCAGGCT 
GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG TGGTGGATGC TGGGAAGGGC 
AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG GCATGATCTT GAAGCAGACC 
TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC TGCTGGAACA TGGCCTGGAA 
CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC TTAAACCAAT GCTGGCTCAT 
CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG AGGTCGACTT CACCTGTGAA 
TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG AAGGTGGAGA AGTGAAGATC 
TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG ACATTATCAG CCGCATCCCC 
AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA CTGAGGCTGT GGCCTGGGAC 
CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA CGCGCAAGCG CAAGGAGGTT 
GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT TTGACCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC AGCTGCTCCG TGAGAACTTC 
GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG ACACCAAGGA CACCGAGCAG 
ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG AGGGCCTGAT GGTGAAGACC 
TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG TGGTGATTGG TGCCTACCTG 
GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT TGGCTGCCTA CGATGAGGAG 
AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT TCAGCGATGA GGAGCTGGAG 
GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC CACGCCCCTA TGTGAGGATT 
GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA TTGTGTGGGA GGTGAAGTGT 
GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG GCCTGGTGGA CAAAGAAAAA 
GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA AAGACAAGCA GCCAGAGCAG 
GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC AGAGTCAGAT TCAGAACCAA 
CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186239.1
CDS	227..3025
Translation	MRKKEQERKGETSAANMQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_001199310.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199310.1

ORF Insert Sequence:

ATGAGAAAAA AAGAGCAAGA GAGGAAAGGA GAGACTTCTG CTGCCAACAT GCAGAGAAGT 
ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA AGAAGCCAGA GAAGGAGACA 
CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG CGCTGAAGGA GAGGAATCAA 
GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA GGAAGGTAGC CCAGGTTCTG 
AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC CCAAAGTCCA GAAGCCTGTG 
TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT GTCCTGAGAA CAGTCCTGTC 
TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG TCGAACTGCG 
CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG AGGAGCAGAA TGAGGACAAA 
ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC CAAAAGAAAG CCTCGCAGAG 
GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC AGCTCATAGT CCCCTCTGAG 
CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG AGAACATTCC AGTGTGTAAG 
GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA AACCTCCTGC TAGAGGTGCC 
AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG TGAAAACTGA AGTCAAACAA 
GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA CCCTGGATCC AGCAAATTAT 
AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT GCTGGAAACA TGGCCAGAAG 
GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG AGGAGGTTTC TGCTAGGCTC 
AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC CCCTCCAGAC 
TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC CACCTCAGCA GGGACTAGAG 
CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC AGGCCACAGG TCGTCAGCTG 
GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG GGCTGGTGGC CGAGAACAGC 
CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA CTATTTCTGG AGTCTTCACC 
AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA TGGCCAAGAA GATGGACATT 
ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA GGTACATTGC CAGGTCCCTA 
AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC TGGCTGCTCT TGCCCAGGCT 
GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG TGGTGGATGC TGGGAAGGGC 
AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG GCATGATCTT GAAGCAGACC 
TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC TGCTGGAACA TGGCCTGGAA 
CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC TTAAACCAAT GCTGGCTCAT 
CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG AGGTCGACTT CACCTGTGAA 
TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG AAGGTGGAGA AGTGAAGATC 
TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG ACATTATCAG CCGCATCCCC 
AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA CTGAGGCTGT GGCCTGGGAC 
CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA CGCGCAAGCG CAAGGAGGTT 
GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT TTGACCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC AGCTGCTCCG TGAGAACTTC 
GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG ACACCAAGGA CACCGAGCAG 
ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG AGGGCCTGAT GGTGAAGACC 
TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG TGGTGATTGG TGCCTACCTG 
GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT TGGCTGCCTA CGATGAGGAG 
AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT TCAGCGATGA GGAGCTGGAG 
GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC CACGCCCCTA TGTGAGGATT 
GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA TTGTGTGGGA GGTGAAGTGT 
GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG GCCTGGTGGA CAAAGAAAAA 
GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA AAGACAAGCA GCCAGAGCAG 
GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC AGAGTCAGAT TCAGAACCAA 
CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig1 ( NM_001083188.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001076657.1 Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02217
Clone ID Related Accession (Same CDS sequence)	NM_001199310.1 , NM_001083188.1 , NM_010715.2
Accession Version	NM_001083188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Mus musculus(house mouse)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK168643.1 and BE996000.1. Transcript Variant: This variant (1) uses a different segment for its 5' UTR, compared to variant 3. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAAAA AAGAGCAAGA GAGGAAAGGA GAGACTTCTG CTGCCAACAT GCAGAGAAGT 
ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA AGAAGCCAGA GAAGGAGACA 
CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG CGCTGAAGGA GAGGAATCAA 
GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA GGAAGGTAGC CCAGGTTCTG 
AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC CCAAAGTCCA GAAGCCTGTG 
TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT GTCCTGAGAA CAGTCCTGTC 
TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG TCGAACTGCG 
CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG AGGAGCAGAA TGAGGACAAA 
ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC CAAAAGAAAG CCTCGCAGAG 
GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC AGCTCATAGT CCCCTCTGAG 
CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG AGAACATTCC AGTGTGTAAG 
GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA AACCTCCTGC TAGAGGTGCC 
AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG TGAAAACTGA AGTCAAACAA 
GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA CCCTGGATCC AGCAAATTAT 
AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT GCTGGAAACA TGGCCAGAAG 
GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG AGGAGGTTTC TGCTAGGCTC 
AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC CCCTCCAGAC 
TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC CACCTCAGCA GGGACTAGAG 
CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC AGGCCACAGG TCGTCAGCTG 
GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG GGCTGGTGGC CGAGAACAGC 
CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA CTATTTCTGG AGTCTTCACC 
AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA TGGCCAAGAA GATGGACATT 
ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA GGTACATTGC CAGGTCCCTA 
AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC TGGCTGCTCT TGCCCAGGCT 
GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG TGGTGGATGC TGGGAAGGGC 
AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG GCATGATCTT GAAGCAGACC 
TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC TGCTGGAACA TGGCCTGGAA 
CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC TTAAACCAAT GCTGGCTCAT 
CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG AGGTCGACTT CACCTGTGAA 
TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG AAGGTGGAGA AGTGAAGATC 
TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG ACATTATCAG CCGCATCCCC 
AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA CTGAGGCTGT GGCCTGGGAC 
CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA CGCGCAAGCG CAAGGAGGTT 
GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT TTGACCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC AGCTGCTCCG TGAGAACTTC 
GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG ACACCAAGGA CACCGAGCAG 
ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG AGGGCCTGAT GGTGAAGACC 
TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG TGGTGATTGG TGCCTACCTG 
GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT TGGCTGCCTA CGATGAGGAG 
AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT TCAGCGATGA GGAGCTGGAG 
GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC CACGCCCCTA TGTGAGGATT 
GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA TTGTGTGGGA GGTGAAGTGT 
GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG GCCTGGTGGA CAAAGAAAAA 
GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA AAGACAAGCA GCCAGAGCAG 
GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC AGAGTCAGAT TCAGAACCAA 
CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076657.1
CDS	219..3017
Translation	MRKKEQERKGETSAANMQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_001083188.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083188.1

ORF Insert Sequence:

ATGAGAAAAA AAGAGCAAGA GAGGAAAGGA GAGACTTCTG CTGCCAACAT GCAGAGAAGT 
ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA AGAAGCCAGA GAAGGAGACA 
CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG CGCTGAAGGA GAGGAATCAA 
GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA GGAAGGTAGC CCAGGTTCTG 
AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC CCAAAGTCCA GAAGCCTGTG 
TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT GTCCTGAGAA CAGTCCTGTC 
TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG TCGAACTGCG 
CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG AGGAGCAGAA TGAGGACAAA 
ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC CAAAAGAAAG CCTCGCAGAG 
GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC AGCTCATAGT CCCCTCTGAG 
CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG AGAACATTCC AGTGTGTAAG 
GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA AACCTCCTGC TAGAGGTGCC 
AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG TGAAAACTGA AGTCAAACAA 
GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA CCCTGGATCC AGCAAATTAT 
AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT GCTGGAAACA TGGCCAGAAG 
GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG AGGAGGTTTC TGCTAGGCTC 
AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC CCCTCCAGAC 
TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC CACCTCAGCA GGGACTAGAG 
CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC AGGCCACAGG TCGTCAGCTG 
GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG GGCTGGTGGC CGAGAACAGC 
CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA CTATTTCTGG AGTCTTCACC 
AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA TGGCCAAGAA GATGGACATT 
ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA GGTACATTGC CAGGTCCCTA 
AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC TGGCTGCTCT TGCCCAGGCT 
GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG TGGTGGATGC TGGGAAGGGC 
AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG GCATGATCTT GAAGCAGACC 
TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC TGCTGGAACA TGGCCTGGAA 
CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC TTAAACCAAT GCTGGCTCAT 
CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG AGGTCGACTT CACCTGTGAA 
TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG AAGGTGGAGA AGTGAAGATC 
TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG ACATTATCAG CCGCATCCCC 
AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA CTGAGGCTGT GGCCTGGGAC 
CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA CGCGCAAGCG CAAGGAGGTT 
GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT TTGACCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC AGCTGCTCCG TGAGAACTTC 
GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG ACACCAAGGA CACCGAGCAG 
ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG AGGGCCTGAT GGTGAAGACC 
TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG TGGTGATTGG TGCCTACCTG 
GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT TGGCTGCCTA CGATGAGGAG 
AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT TCAGCGATGA GGAGCTGGAG 
GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC CACGCCCCTA TGTGAGGATT 
GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA TTGTGTGGGA GGTGAAGTGT 
GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG GCCTGGTGGA CAAAGAAAAA 
GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA AAGACAAGCA GCCAGAGCAG 
GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC AGAGTCAGAT TCAGAACCAA 
CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig1 ( NM_010715.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034845.2 Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 2, mRNA.

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CloneID	OMu02217
Clone ID Related Accession (Same CDS sequence)	NM_001199310.1 , NM_001083188.1 , NM_010715.2
Accession Version	NM_010715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-11
Organism	Mus musculus(house mouse)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY099263.1, U04674.1, AC161792.9, AK161793.1 and BE996000.1. On Mar 21, 2007 this sequence version replaced NM_010715.1. Transcript Variant: This variant (2) uses a different segment for its 5' UTR, compared to variant 3. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153993.1, BC028287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAAAA AAGAGCAAGA GAGGAAAGGA GAGACTTCTG CTGCCAACAT GCAGAGAAGT 
ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA AGAAGCCAGA GAAGGAGACA 
CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG CGCTGAAGGA GAGGAATCAA 
GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA GGAAGGTAGC CCAGGTTCTG 
AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC CCAAAGTCCA GAAGCCTGTG 
TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT GTCCTGAGAA CAGTCCTGTC 
TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG TCGAACTGCG 
CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG AGGAGCAGAA TGAGGACAAA 
ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC CAAAAGAAAG CCTCGCAGAG 
GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC AGCTCATAGT CCCCTCTGAG 
CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG AGAACATTCC AGTGTGTAAG 
GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA AACCTCCTGC TAGAGGTGCC 
AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG TGAAAACTGA AGTCAAACAA 
GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA CCCTGGATCC AGCAAATTAT 
AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT GCTGGAAACA TGGCCAGAAG 
GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG AGGAGGTTTC TGCTAGGCTC 
AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC CCCTCCAGAC 
TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC CACCTCAGCA GGGACTAGAG 
CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC AGGCCACAGG TCGTCAGCTG 
GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG GGCTGGTGGC CGAGAACAGC 
CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA CTATTTCTGG AGTCTTCACC 
AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA TGGCCAAGAA GATGGACATT 
ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA GGTACATTGC CAGGTCCCTA 
AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC TGGCTGCTCT TGCCCAGGCT 
GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG TGGTGGATGC TGGGAAGGGC 
AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG GCATGATCTT GAAGCAGACC 
TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC TGCTGGAACA TGGCCTGGAA 
CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC TTAAACCAAT GCTGGCTCAT 
CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG AGGTCGACTT CACCTGTGAA 
TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG AAGGTGGAGA AGTGAAGATC 
TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG ACATTATCAG CCGCATCCCC 
AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA CTGAGGCTGT GGCCTGGGAC 
CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA CGCGCAAGCG CAAGGAGGTT 
GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT TTGACCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC AGCTGCTCCG TGAGAACTTC 
GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG ACACCAAGGA CACCGAGCAG 
ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG AGGGCCTGAT GGTGAAGACC 
TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG TGGTGATTGG TGCCTACCTG 
GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT TGGCTGCCTA CGATGAGGAG 
AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT TCAGCGATGA GGAGCTGGAG 
GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC CACGCCCCTA TGTGAGGATT 
GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA TTGTGTGGGA GGTGAAGTGT 
GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG GCCTGGTGGA CAAAGAAAAA 
GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA AAGACAAGCA GCCAGAGCAG 
GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC AGAGTCAGAT TCAGAACCAA 
CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034845.2
CDS	144..2942
Translation	MRKKEQERKGETSAANMQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_010715.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010715.2

ORF Insert Sequence:

ATGAGAAAAA AAGAGCAAGA GAGGAAAGGA GAGACTTCTG CTGCCAACAT GCAGAGAAGT 
ATTATGTCAT TTTTTCAACC CACAAAAGAA GGTAAAGCGA AGAAGCCAGA GAAGGAGACA 
CCCAGCAGCA TCAGAGAGAA GGAACCCCCT CCAAAGGTGG CGCTGAAGGA GAGGAATCAA 
GTGGTGCCCG AGAGTGATTC TCCAGTGAAG AGGACAGGAA GGAAGGTAGC CCAGGTTCTG 
AGCTGTGAAG GGGAGGACGA AGATGAAGCC CCTGGCACCC CCAAAGTCCA GAAGCCTGTG 
TCAGACTCTG AACAGAGCTC TCCTCCCAGC CCTGACACAT GTCCTGAGAA CAGTCCTGTC 
TTCAACTGCA GCTCCCCCAT GGACATCTCC CCATCAGGAT TCCCAAAGCG TCGAACTGCG 
CGGAAGCAGC TCCCCAAACG GACAATTCAA GACACTCTTG AGGAGCAGAA TGAGGACAAA 
ACCAAAACAG CTAAGAAAAG GAAGAAAGAA GAAGAGACCC CAAAAGAAAG CCTCGCAGAG 
GCTGAAGACG TAAAACAGAA GGAAGAAAAG GAGGGGGACC AGCTCATAGT CCCCTCTGAG 
CCCACAAAGT CCCCTGAGTC AGTAACCCTG ACAAAGACAG AGAACATTCC AGTGTGTAAG 
GCAGGAGTGA AACTGAAGCC TCAGGAAGAG GAACAGAGCA AACCTCCTGC TAGAGGTGCC 
AAGACACTGA GCAGTTTCTT CACACCCCGA AAACCAGCAG TGAAAACTGA AGTCAAACAA 
GAAGAGTCAG GCACTCTAAG GAAGGAAGAG ACCAAGGGAA CCCTGGATCC AGCAAATTAT 
AATCCTTCCA AGAATAACTA TCATCCCATT GAAGATGCCT GCTGGAAACA TGGCCAGAAG 
GTCCCTTTTC TCGCCGTGGC CCGGACATTT GAGAAGATTG AGGAGGTTTC TGCTAGGCTC 
AAGATGGTGG AGACATTGAG CAACCTGCTG CGCTCCGTGG TGGCCCTGTC CCCTCCAGAC 
TTGCTCCCTG TTCTCTATCT CAGCCTCAAC CGCCTCGGGC CACCTCAGCA GGGACTAGAG 
CTGGGTGTTG GTGATGGTGT CCTCCTCAAG GCCGTTGCCC AGGCCACAGG TCGTCAGCTG 
GAGTCCATCC GGGCTGAGGT AGCTGAGAAG GGGGATGTGG GGCTGGTGGC CGAGAACAGC 
CGCAGCACTC AGAGACTCAT GCTGCCTCCA CCTCCACTTA CTATTTCTGG AGTCTTCACC 
AAATTCTGTG ACATTGCCCG GCTCACTGGC AGTGCTTCCA TGGCCAAGAA GATGGACATT 
ATCAAGGGCC TTTTTGTTGC CTGCCGCCAC TCAGAAGCCA GGTACATTGC CAGGTCCCTA 
AGCGGGCGGC TGCGCCTTGG CCTAGCTGAG CAGTCGGTAC TGGCTGCTCT TGCCCAGGCT 
GTGAGCCTCA CACCCCCTGG CCAAGAATTT CCCACGGCGG TGGTGGATGC TGGGAAGGGC 
AAGACAGCAG AGGCCAGAAA GATGTGGTTG GAAGAACAAG GCATGATCTT GAAGCAGACC 
TTCTGTGAGG TTCCTGACCT GGATCGAATC ATCCCTGTGC TGCTGGAACA TGGCCTGGAA 
CGTCTCCCAG AGCACTGCAA GCTGAGCCCA GGGGTCCCTC TTAAACCAAT GCTGGCTCAT 
CCCACTCGGG GTGTCAGTGA GGTCCTAAAA CGCTTTGAGG AGGTCGACTT CACCTGTGAA 
TATAAATACG ATGGGCAGCG AGCACAGATA CATGTTCTGG AAGGTGGAGA AGTGAAGATC 
TTCAGCAGGA ACCAGGAAGA CAACACAGGA AAGTATCCTG ACATTATCAG CCGCATCCCC 
AAGATTAAAC ACCCCTCAGT CACATCTTTC ATTCTGGACA CTGAGGCTGT GGCCTGGGAC 
CGGGAAAAGA AGCAGATCCA ACCATTCCAA GTACTCACCA CGCGCAAGCG CAAGGAGGTT 
GATGCATCGG AGATACAGGT GCAAGTGTGT CTGTATGCTT TTGACCTCAT CTACCTCAAC 
GGAGAGTCCC TGGTTCGCCA GCCCCTGTCT CGACGCCGGC AGCTGCTCCG TGAGAACTTC 
GTGGAGACAG AGGGTGAGTT TGTCTTCACC ACCTCTTTGG ACACCAAGGA CACCGAGCAG 
ATTGCTGAGT TCTTGGAGCA GTCAGTGAAG GACTCCTGTG AGGGCCTGAT GGTGAAGACC 
TTGGATGTTG ATGCCACTTA TGAGATTGCC AAGAGGTCAC ACAACTGGCT CAAGCTGAAG 
AAGGACTACC TTGACGGTGT GGGTGACACT CTGGACCTTG TGGTGATTGG TGCCTACCTG 
GGCCGGGGGA AGCGGGCTGG CCGGTATGGA GGCTTCCTGT TGGCTGCCTA CGATGAGGAG 
AGTGAAGAGC TTCAGGCTAT ATGCAAGCTG GGAACTGGAT TCAGCGATGA GGAGCTGGAG 
GAACACCACC AGAGCCTGCA GGCCCTGGTA TTGCCTACCC CACGCCCCTA TGTGAGGATT 
GATGGGGCAG TGGCCCCAGA CCATTGGCTG GACCCAAGCA TTGTGTGGGA GGTGAAGTGT 
GCTGATCTTT CCCTGTCCCC CATCTACCCT GCTGCACGGG GCCTGGTGGA CAAAGAAAAA 
GGGATCTCTC TTCGTTTTCC TCGGTTCATT CGTGTCCGTA AAGACAAGCA GCCAGAGCAG 
GCCACTACCA GTAACCAGGT TGCCTCTTTG TACCGGAAGC AGAGTCAGAT TCAGAACCAA 
CAAAGCTCAG ACTTGGACTC CGACGTTGAA GACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig1 ( NM_010715.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034845.3 Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu90644
Clone ID Related Accession (Same CDS sequence)	NM_010715.3 , NM_001199310.2 , NM_001083188.2
Accession Version	NM_010715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161792.9 and AK161793.1. On Dec 5, 2019 this sequence version replaced NM_010715.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028287.1, AK153993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGAA GTATTATGTC ATTTTTTCAA CCCACAAAAG AAGGTAAAGC GAAGAAGCCA 
GAGAAGGAGA CACCCAGCAG CATCAGAGAG AAGGAACCCC CTCCAAAGGT GGCGCTGAAG 
GAGAGGAATC AAGTGGTGCC CGAGAGTGAT TCTCCAGTGA AGAGGACAGG AAGGAAGGTA 
GCCCAGGTTC TGAGCTGTGA AGGGGAGGAC GAAGATGAAG CCCCTGGCAC CCCCAAAGTC 
CAGAAGCCTG TGTCAGACTC TGAACAGAGC TCTCCTCCCA GCCCTGACAC ATGTCCTGAG 
AACAGTCCTG TCTTCAACTG CAGCTCCCCC ATGGACATCT CCCCATCAGG ATTCCCAAAG 
CGTCGAACTG CGCGGAAGCA GCTCCCCAAA CGGACAATTC AAGACACTCT TGAGGAGCAG 
AATGAGGACA AAACCAAAAC AGCTAAGAAA AGGAAGAAAG AAGAAGAGAC CCCAAAAGAA 
AGCCTCGCAG AGGCTGAAGA CGTAAAACAG AAGGAAGAAA AGGAGGGGGA CCAGCTCATA 
GTCCCCTCTG AGCCCACAAA GTCCCCTGAG TCAGTAACCC TGACAAAGAC AGAGAACATT 
CCAGTGTGTA AGGCAGGAGT GAAACTGAAG CCTCAGGAAG AGGAACAGAG CAAACCTCCT 
GCTAGAGGTG CCAAGACACT GAGCAGTTTC TTCACACCCC GAAAACCAGC AGTGAAAACT 
GAAGTCAAAC AAGAAGAGTC AGGCACTCTA AGGAAGGAAG AGACCAAGGG AACCCTGGAT 
CCAGCAAATT ATAATCCTTC CAAGAATAAC TATCATCCCA TTGAAGATGC CTGCTGGAAA 
CATGGCCAGA AGGTCCCTTT TCTCGCCGTG GCCCGGACAT TTGAGAAGAT TGAGGAGGTT 
TCTGCTAGGC TCAAGATGGT GGAGACATTG AGCAACCTGC TGCGCTCCGT GGTGGCCCTG 
TCCCCTCCAG ACTTGCTCCC TGTTCTCTAT CTCAGCCTCA ACCGCCTCGG GCCACCTCAG 
CAGGGACTAG AGCTGGGTGT TGGTGATGGT GTCCTCCTCA AGGCCGTTGC CCAGGCCACA 
GGTCGTCAGC TGGAGTCCAT CCGGGCTGAG GTAGCTGAGA AGGGGGATGT GGGGCTGGTG 
GCCGAGAACA GCCGCAGCAC TCAGAGACTC ATGCTGCCTC CACCTCCACT TACTATTTCT 
GGAGTCTTCA CCAAATTCTG TGACATTGCC CGGCTCACTG GCAGTGCTTC CATGGCCAAG 
AAGATGGACA TTATCAAGGG CCTTTTTGTT GCCTGCCGCC ACTCAGAAGC CAGGTACATT 
GCCAGGTCCC TAAGCGGGCG GCTGCGCCTT GGCCTAGCTG AGCAGTCGGT ACTGGCTGCT 
CTTGCCCAGG CTGTGAGCCT CACACCCCCT GGCCAAGAAT TTCCCACGGC GGTGGTGGAT 
GCTGGGAAGG GCAAGACAGC AGAGGCCAGA AAGATGTGGT TGGAAGAACA AGGCATGATC 
TTGAAGCAGA CCTTCTGTGA GGTTCCTGAC CTGGATCGAA TCATCCCTGT GCTGCTGGAA 
CATGGCCTGG AACGTCTCCC AGAGCACTGC AAGCTGAGCC CAGGGGTCCC TCTTAAACCA 
ATGCTGGCTC ATCCCACTCG GGGTGTCAGT GAGGTCCTAA AACGCTTTGA GGAGGTCGAC 
TTCACCTGTG AATATAAATA CGATGGGCAG CGAGCACAGA TACATGTTCT GGAAGGTGGA 
GAAGTGAAGA TCTTCAGCAG GAACCAGGAA GACAACACAG GAAAGTATCC TGACATTATC 
AGCCGCATCC CCAAGATTAA ACACCCCTCA GTCACATCTT TCATTCTGGA CACTGAGGCT 
GTGGCCTGGG ACCGGGAAAA GAAGCAGATC CAACCATTCC AAGTACTCAC CACGCGCAAG 
CGCAAGGAGG TTGATGCATC GGAGATACAG GTGCAAGTGT GTCTGTATGC TTTTGACCTC 
ATCTACCTCA ACGGAGAGTC CCTGGTTCGC CAGCCCCTGT CTCGACGCCG GCAGCTGCTC 
CGTGAGAACT TCGTGGAGAC AGAGGGTGAG TTTGTCTTCA CCACCTCTTT GGACACCAAG 
GACACCGAGC AGATTGCTGA GTTCTTGGAG CAGTCAGTGA AGGACTCCTG TGAGGGCCTG 
ATGGTGAAGA CCTTGGATGT TGATGCCACT TATGAGATTG CCAAGAGGTC ACACAACTGG 
CTCAAGCTGA AGAAGGACTA CCTTGACGGT GTGGGTGACA CTCTGGACCT TGTGGTGATT 
GGTGCCTACC TGGGCCGGGG GAAGCGGGCT GGCCGGTATG GAGGCTTCCT GTTGGCTGCC 
TACGATGAGG AGAGTGAAGA GCTTCAGGCT ATATGCAAGC TGGGAACTGG ATTCAGCGAT 
GAGGAGCTGG AGGAACACCA CCAGAGCCTG CAGGCCCTGG TATTGCCTAC CCCACGCCCC 
TATGTGAGGA TTGATGGGGC AGTGGCCCCA GACCATTGGC TGGACCCAAG CATTGTGTGG 
GAGGTGAAGT GTGCTGATCT TTCCCTGTCC CCCATCTACC CTGCTGCACG GGGCCTGGTG 
GACAAAGAAA AAGGGATCTC TCTTCGTTTT CCTCGGTTCA TTCGTGTCCG TAAAGACAAG 
CAGCCAGAGC AGGCCACTAC CAGTAACCAG GTTGCCTCTT TGTACCGGAA GCAGAGTCAG 
ATTCAGAACC AACAAAGCTC AGACTTGGAC TCCGACGTTG AAGACTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034845.3
CDS	192..2942
Translation	MQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_010715.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010715.3

ORF Insert Sequence:

ATGCAGAGAA GTATTATGTC ATTTTTTCAA CCCACAAAAG AAGGTAAAGC GAAGAAGCCA 
GAGAAGGAGA CACCCAGCAG CATCAGAGAG AAGGAACCCC CTCCAAAGGT GGCGCTGAAG 
GAGAGGAATC AAGTGGTGCC CGAGAGTGAT TCTCCAGTGA AGAGGACAGG AAGGAAGGTA 
GCCCAGGTTC TGAGCTGTGA AGGGGAGGAC GAAGATGAAG CCCCTGGCAC CCCCAAAGTC 
CAGAAGCCTG TGTCAGACTC TGAACAGAGC TCTCCTCCCA GCCCTGACAC ATGTCCTGAG 
AACAGTCCTG TCTTCAACTG CAGCTCCCCC ATGGACATCT CCCCATCAGG ATTCCCAAAG 
CGTCGAACTG CGCGGAAGCA GCTCCCCAAA CGGACAATTC AAGACACTCT TGAGGAGCAG 
AATGAGGACA AAACCAAAAC AGCTAAGAAA AGGAAGAAAG AAGAAGAGAC CCCAAAAGAA 
AGCCTCGCAG AGGCTGAAGA CGTAAAACAG AAGGAAGAAA AGGAGGGGGA CCAGCTCATA 
GTCCCCTCTG AGCCCACAAA GTCCCCTGAG TCAGTAACCC TGACAAAGAC AGAGAACATT 
CCAGTGTGTA AGGCAGGAGT GAAACTGAAG CCTCAGGAAG AGGAACAGAG CAAACCTCCT 
GCTAGAGGTG CCAAGACACT GAGCAGTTTC TTCACACCCC GAAAACCAGC AGTGAAAACT 
GAAGTCAAAC AAGAAGAGTC AGGCACTCTA AGGAAGGAAG AGACCAAGGG AACCCTGGAT 
CCAGCAAATT ATAATCCTTC CAAGAATAAC TATCATCCCA TTGAAGATGC CTGCTGGAAA 
CATGGCCAGA AGGTCCCTTT TCTCGCCGTG GCCCGGACAT TTGAGAAGAT TGAGGAGGTT 
TCTGCTAGGC TCAAGATGGT GGAGACATTG AGCAACCTGC TGCGCTCCGT GGTGGCCCTG 
TCCCCTCCAG ACTTGCTCCC TGTTCTCTAT CTCAGCCTCA ACCGCCTCGG GCCACCTCAG 
CAGGGACTAG AGCTGGGTGT TGGTGATGGT GTCCTCCTCA AGGCCGTTGC CCAGGCCACA 
GGTCGTCAGC TGGAGTCCAT CCGGGCTGAG GTAGCTGAGA AGGGGGATGT GGGGCTGGTG 
GCCGAGAACA GCCGCAGCAC TCAGAGACTC ATGCTGCCTC CACCTCCACT TACTATTTCT 
GGAGTCTTCA CCAAATTCTG TGACATTGCC CGGCTCACTG GCAGTGCTTC CATGGCCAAG 
AAGATGGACA TTATCAAGGG CCTTTTTGTT GCCTGCCGCC ACTCAGAAGC CAGGTACATT 
GCCAGGTCCC TAAGCGGGCG GCTGCGCCTT GGCCTAGCTG AGCAGTCGGT ACTGGCTGCT 
CTTGCCCAGG CTGTGAGCCT CACACCCCCT GGCCAAGAAT TTCCCACGGC GGTGGTGGAT 
GCTGGGAAGG GCAAGACAGC AGAGGCCAGA AAGATGTGGT TGGAAGAACA AGGCATGATC 
TTGAAGCAGA CCTTCTGTGA GGTTCCTGAC CTGGATCGAA TCATCCCTGT GCTGCTGGAA 
CATGGCCTGG AACGTCTCCC AGAGCACTGC AAGCTGAGCC CAGGGGTCCC TCTTAAACCA 
ATGCTGGCTC ATCCCACTCG GGGTGTCAGT GAGGTCCTAA AACGCTTTGA GGAGGTCGAC 
TTCACCTGTG AATATAAATA CGATGGGCAG CGAGCACAGA TACATGTTCT GGAAGGTGGA 
GAAGTGAAGA TCTTCAGCAG GAACCAGGAA GACAACACAG GAAAGTATCC TGACATTATC 
AGCCGCATCC CCAAGATTAA ACACCCCTCA GTCACATCTT TCATTCTGGA CACTGAGGCT 
GTGGCCTGGG ACCGGGAAAA GAAGCAGATC CAACCATTCC AAGTACTCAC CACGCGCAAG 
CGCAAGGAGG TTGATGCATC GGAGATACAG GTGCAAGTGT GTCTGTATGC TTTTGACCTC 
ATCTACCTCA ACGGAGAGTC CCTGGTTCGC CAGCCCCTGT CTCGACGCCG GCAGCTGCTC 
CGTGAGAACT TCGTGGAGAC AGAGGGTGAG TTTGTCTTCA CCACCTCTTT GGACACCAAG 
GACACCGAGC AGATTGCTGA GTTCTTGGAG CAGTCAGTGA AGGACTCCTG TGAGGGCCTG 
ATGGTGAAGA CCTTGGATGT TGATGCCACT TATGAGATTG CCAAGAGGTC ACACAACTGG 
CTCAAGCTGA AGAAGGACTA CCTTGACGGT GTGGGTGACA CTCTGGACCT TGTGGTGATT 
GGTGCCTACC TGGGCCGGGG GAAGCGGGCT GGCCGGTATG GAGGCTTCCT GTTGGCTGCC 
TACGATGAGG AGAGTGAAGA GCTTCAGGCT ATATGCAAGC TGGGAACTGG ATTCAGCGAT 
GAGGAGCTGG AGGAACACCA CCAGAGCCTG CAGGCCCTGG TATTGCCTAC CCCACGCCCC 
TATGTGAGGA TTGATGGGGC AGTGGCCCCA GACCATTGGC TGGACCCAAG CATTGTGTGG 
GAGGTGAAGT GTGCTGATCT TTCCCTGTCC CCCATCTACC CTGCTGCACG GGGCCTGGTG 
GACAAAGAAA AAGGGATCTC TCTTCGTTTT CCTCGGTTCA TTCGTGTCCG TAAAGACAAG 
CAGCCAGAGC AGGCCACTAC CAGTAACCAG GTTGCCTCTT TGTACCGGAA GCAGAGTCAG 
ATTCAGAACC AACAAAGCTC AGACTTGGAC TCCGACGTTG AAGACTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig1 ( NM_001199310.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186239.2 Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 3, mRNA.

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CloneID	OMu90644
Clone ID Related Accession (Same CDS sequence)	NM_010715.3 , NM_001199310.2 , NM_001083188.2
Accession Version	NM_001199310.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161792.9 and AK161793.1. On Dec 5, 2019 this sequence version replaced NM_001199310.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138542.1, U19604.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCAGAGAA GTATTATGTC ATTTTTTCAA CCCACAAAAG AAGGTAAAGC GAAGAAGCCA 
GAGAAGGAGA CACCCAGCAG CATCAGAGAG AAGGAACCCC CTCCAAAGGT GGCGCTGAAG 
GAGAGGAATC AAGTGGTGCC CGAGAGTGAT TCTCCAGTGA AGAGGACAGG AAGGAAGGTA 
GCCCAGGTTC TGAGCTGTGA AGGGGAGGAC GAAGATGAAG CCCCTGGCAC CCCCAAAGTC 
CAGAAGCCTG TGTCAGACTC TGAACAGAGC TCTCCTCCCA GCCCTGACAC ATGTCCTGAG 
AACAGTCCTG TCTTCAACTG CAGCTCCCCC ATGGACATCT CCCCATCAGG ATTCCCAAAG 
CGTCGAACTG CGCGGAAGCA GCTCCCCAAA CGGACAATTC AAGACACTCT TGAGGAGCAG 
AATGAGGACA AAACCAAAAC AGCTAAGAAA AGGAAGAAAG AAGAAGAGAC CCCAAAAGAA 
AGCCTCGCAG AGGCTGAAGA CGTAAAACAG AAGGAAGAAA AGGAGGGGGA CCAGCTCATA 
GTCCCCTCTG AGCCCACAAA GTCCCCTGAG TCAGTAACCC TGACAAAGAC AGAGAACATT 
CCAGTGTGTA AGGCAGGAGT GAAACTGAAG CCTCAGGAAG AGGAACAGAG CAAACCTCCT 
GCTAGAGGTG CCAAGACACT GAGCAGTTTC TTCACACCCC GAAAACCAGC AGTGAAAACT 
GAAGTCAAAC AAGAAGAGTC AGGCACTCTA AGGAAGGAAG AGACCAAGGG AACCCTGGAT 
CCAGCAAATT ATAATCCTTC CAAGAATAAC TATCATCCCA TTGAAGATGC CTGCTGGAAA 
CATGGCCAGA AGGTCCCTTT TCTCGCCGTG GCCCGGACAT TTGAGAAGAT TGAGGAGGTT 
TCTGCTAGGC TCAAGATGGT GGAGACATTG AGCAACCTGC TGCGCTCCGT GGTGGCCCTG 
TCCCCTCCAG ACTTGCTCCC TGTTCTCTAT CTCAGCCTCA ACCGCCTCGG GCCACCTCAG 
CAGGGACTAG AGCTGGGTGT TGGTGATGGT GTCCTCCTCA AGGCCGTTGC CCAGGCCACA 
GGTCGTCAGC TGGAGTCCAT CCGGGCTGAG GTAGCTGAGA AGGGGGATGT GGGGCTGGTG 
GCCGAGAACA GCCGCAGCAC TCAGAGACTC ATGCTGCCTC CACCTCCACT TACTATTTCT 
GGAGTCTTCA CCAAATTCTG TGACATTGCC CGGCTCACTG GCAGTGCTTC CATGGCCAAG 
AAGATGGACA TTATCAAGGG CCTTTTTGTT GCCTGCCGCC ACTCAGAAGC CAGGTACATT 
GCCAGGTCCC TAAGCGGGCG GCTGCGCCTT GGCCTAGCTG AGCAGTCGGT ACTGGCTGCT 
CTTGCCCAGG CTGTGAGCCT CACACCCCCT GGCCAAGAAT TTCCCACGGC GGTGGTGGAT 
GCTGGGAAGG GCAAGACAGC AGAGGCCAGA AAGATGTGGT TGGAAGAACA AGGCATGATC 
TTGAAGCAGA CCTTCTGTGA GGTTCCTGAC CTGGATCGAA TCATCCCTGT GCTGCTGGAA 
CATGGCCTGG AACGTCTCCC AGAGCACTGC AAGCTGAGCC CAGGGGTCCC TCTTAAACCA 
ATGCTGGCTC ATCCCACTCG GGGTGTCAGT GAGGTCCTAA AACGCTTTGA GGAGGTCGAC 
TTCACCTGTG AATATAAATA CGATGGGCAG CGAGCACAGA TACATGTTCT GGAAGGTGGA 
GAAGTGAAGA TCTTCAGCAG GAACCAGGAA GACAACACAG GAAAGTATCC TGACATTATC 
AGCCGCATCC CCAAGATTAA ACACCCCTCA GTCACATCTT TCATTCTGGA CACTGAGGCT 
GTGGCCTGGG ACCGGGAAAA GAAGCAGATC CAACCATTCC AAGTACTCAC CACGCGCAAG 
CGCAAGGAGG TTGATGCATC GGAGATACAG GTGCAAGTGT GTCTGTATGC TTTTGACCTC 
ATCTACCTCA ACGGAGAGTC CCTGGTTCGC CAGCCCCTGT CTCGACGCCG GCAGCTGCTC 
CGTGAGAACT TCGTGGAGAC AGAGGGTGAG TTTGTCTTCA CCACCTCTTT GGACACCAAG 
GACACCGAGC AGATTGCTGA GTTCTTGGAG CAGTCAGTGA AGGACTCCTG TGAGGGCCTG 
ATGGTGAAGA CCTTGGATGT TGATGCCACT TATGAGATTG CCAAGAGGTC ACACAACTGG 
CTCAAGCTGA AGAAGGACTA CCTTGACGGT GTGGGTGACA CTCTGGACCT TGTGGTGATT 
GGTGCCTACC TGGGCCGGGG GAAGCGGGCT GGCCGGTATG GAGGCTTCCT GTTGGCTGCC 
TACGATGAGG AGAGTGAAGA GCTTCAGGCT ATATGCAAGC TGGGAACTGG ATTCAGCGAT 
GAGGAGCTGG AGGAACACCA CCAGAGCCTG CAGGCCCTGG TATTGCCTAC CCCACGCCCC 
TATGTGAGGA TTGATGGGGC AGTGGCCCCA GACCATTGGC TGGACCCAAG CATTGTGTGG 
GAGGTGAAGT GTGCTGATCT TTCCCTGTCC CCCATCTACC CTGCTGCACG GGGCCTGGTG 
GACAAAGAAA AAGGGATCTC TCTTCGTTTT CCTCGGTTCA TTCGTGTCCG TAAAGACAAG 
CAGCCAGAGC AGGCCACTAC CAGTAACCAG GTTGCCTCTT TGTACCGGAA GCAGAGTCAG 
ATTCAGAACC AACAAAGCTC AGACTTGGAC TCCGACGTTG AAGACTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186239.2
CDS	413..3163
Translation	MQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_001199310.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199310.2

ORF Insert Sequence:

ATGCAGAGAA GTATTATGTC ATTTTTTCAA CCCACAAAAG AAGGTAAAGC GAAGAAGCCA 
GAGAAGGAGA CACCCAGCAG CATCAGAGAG AAGGAACCCC CTCCAAAGGT GGCGCTGAAG 
GAGAGGAATC AAGTGGTGCC CGAGAGTGAT TCTCCAGTGA AGAGGACAGG AAGGAAGGTA 
GCCCAGGTTC TGAGCTGTGA AGGGGAGGAC GAAGATGAAG CCCCTGGCAC CCCCAAAGTC 
CAGAAGCCTG TGTCAGACTC TGAACAGAGC TCTCCTCCCA GCCCTGACAC ATGTCCTGAG 
AACAGTCCTG TCTTCAACTG CAGCTCCCCC ATGGACATCT CCCCATCAGG ATTCCCAAAG 
CGTCGAACTG CGCGGAAGCA GCTCCCCAAA CGGACAATTC AAGACACTCT TGAGGAGCAG 
AATGAGGACA AAACCAAAAC AGCTAAGAAA AGGAAGAAAG AAGAAGAGAC CCCAAAAGAA 
AGCCTCGCAG AGGCTGAAGA CGTAAAACAG AAGGAAGAAA AGGAGGGGGA CCAGCTCATA 
GTCCCCTCTG AGCCCACAAA GTCCCCTGAG TCAGTAACCC TGACAAAGAC AGAGAACATT 
CCAGTGTGTA AGGCAGGAGT GAAACTGAAG CCTCAGGAAG AGGAACAGAG CAAACCTCCT 
GCTAGAGGTG CCAAGACACT GAGCAGTTTC TTCACACCCC GAAAACCAGC AGTGAAAACT 
GAAGTCAAAC AAGAAGAGTC AGGCACTCTA AGGAAGGAAG AGACCAAGGG AACCCTGGAT 
CCAGCAAATT ATAATCCTTC CAAGAATAAC TATCATCCCA TTGAAGATGC CTGCTGGAAA 
CATGGCCAGA AGGTCCCTTT TCTCGCCGTG GCCCGGACAT TTGAGAAGAT TGAGGAGGTT 
TCTGCTAGGC TCAAGATGGT GGAGACATTG AGCAACCTGC TGCGCTCCGT GGTGGCCCTG 
TCCCCTCCAG ACTTGCTCCC TGTTCTCTAT CTCAGCCTCA ACCGCCTCGG GCCACCTCAG 
CAGGGACTAG AGCTGGGTGT TGGTGATGGT GTCCTCCTCA AGGCCGTTGC CCAGGCCACA 
GGTCGTCAGC TGGAGTCCAT CCGGGCTGAG GTAGCTGAGA AGGGGGATGT GGGGCTGGTG 
GCCGAGAACA GCCGCAGCAC TCAGAGACTC ATGCTGCCTC CACCTCCACT TACTATTTCT 
GGAGTCTTCA CCAAATTCTG TGACATTGCC CGGCTCACTG GCAGTGCTTC CATGGCCAAG 
AAGATGGACA TTATCAAGGG CCTTTTTGTT GCCTGCCGCC ACTCAGAAGC CAGGTACATT 
GCCAGGTCCC TAAGCGGGCG GCTGCGCCTT GGCCTAGCTG AGCAGTCGGT ACTGGCTGCT 
CTTGCCCAGG CTGTGAGCCT CACACCCCCT GGCCAAGAAT TTCCCACGGC GGTGGTGGAT 
GCTGGGAAGG GCAAGACAGC AGAGGCCAGA AAGATGTGGT TGGAAGAACA AGGCATGATC 
TTGAAGCAGA CCTTCTGTGA GGTTCCTGAC CTGGATCGAA TCATCCCTGT GCTGCTGGAA 
CATGGCCTGG AACGTCTCCC AGAGCACTGC AAGCTGAGCC CAGGGGTCCC TCTTAAACCA 
ATGCTGGCTC ATCCCACTCG GGGTGTCAGT GAGGTCCTAA AACGCTTTGA GGAGGTCGAC 
TTCACCTGTG AATATAAATA CGATGGGCAG CGAGCACAGA TACATGTTCT GGAAGGTGGA 
GAAGTGAAGA TCTTCAGCAG GAACCAGGAA GACAACACAG GAAAGTATCC TGACATTATC 
AGCCGCATCC CCAAGATTAA ACACCCCTCA GTCACATCTT TCATTCTGGA CACTGAGGCT 
GTGGCCTGGG ACCGGGAAAA GAAGCAGATC CAACCATTCC AAGTACTCAC CACGCGCAAG 
CGCAAGGAGG TTGATGCATC GGAGATACAG GTGCAAGTGT GTCTGTATGC TTTTGACCTC 
ATCTACCTCA ACGGAGAGTC CCTGGTTCGC CAGCCCCTGT CTCGACGCCG GCAGCTGCTC 
CGTGAGAACT TCGTGGAGAC AGAGGGTGAG TTTGTCTTCA CCACCTCTTT GGACACCAAG 
GACACCGAGC AGATTGCTGA GTTCTTGGAG CAGTCAGTGA AGGACTCCTG TGAGGGCCTG 
ATGGTGAAGA CCTTGGATGT TGATGCCACT TATGAGATTG CCAAGAGGTC ACACAACTGG 
CTCAAGCTGA AGAAGGACTA CCTTGACGGT GTGGGTGACA CTCTGGACCT TGTGGTGATT 
GGTGCCTACC TGGGCCGGGG GAAGCGGGCT GGCCGGTATG GAGGCTTCCT GTTGGCTGCC 
TACGATGAGG AGAGTGAAGA GCTTCAGGCT ATATGCAAGC TGGGAACTGG ATTCAGCGAT 
GAGGAGCTGG AGGAACACCA CCAGAGCCTG CAGGCCCTGG TATTGCCTAC CCCACGCCCC 
TATGTGAGGA TTGATGGGGC AGTGGCCCCA GACCATTGGC TGGACCCAAG CATTGTGTGG 
GAGGTGAAGT GTGCTGATCT TTCCCTGTCC CCCATCTACC CTGCTGCACG GGGCCTGGTG 
GACAAAGAAA AAGGGATCTC TCTTCGTTTT CCTCGGTTCA TTCGTGTCCG TAAAGACAAG 
CAGCCAGAGC AGGCCACTAC CAGTAACCAG GTTGCCTCTT TGTACCGGAA GCAGAGTCAG 
ATTCAGAACC AACAAAGCTC AGACTTGGAC TCCGACGTTG AAGACTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lig1 ( NM_001083188.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001076657.2 Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu90644
Clone ID Related Accession (Same CDS sequence)	NM_010715.3 , NM_001199310.2 , NM_001083188.2
Accession Version	NM_001083188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-04
Organism	Mus musculus(house mouse)
Product	DNA ligase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC161792.9 and AK161793.1. On Dec 5, 2019 this sequence version replaced NM_001083188.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGAA GTATTATGTC ATTTTTTCAA CCCACAAAAG AAGGTAAAGC GAAGAAGCCA 
GAGAAGGAGA CACCCAGCAG CATCAGAGAG AAGGAACCCC CTCCAAAGGT GGCGCTGAAG 
GAGAGGAATC AAGTGGTGCC CGAGAGTGAT TCTCCAGTGA AGAGGACAGG AAGGAAGGTA 
GCCCAGGTTC TGAGCTGTGA AGGGGAGGAC GAAGATGAAG CCCCTGGCAC CCCCAAAGTC 
CAGAAGCCTG TGTCAGACTC TGAACAGAGC TCTCCTCCCA GCCCTGACAC ATGTCCTGAG 
AACAGTCCTG TCTTCAACTG CAGCTCCCCC ATGGACATCT CCCCATCAGG ATTCCCAAAG 
CGTCGAACTG CGCGGAAGCA GCTCCCCAAA CGGACAATTC AAGACACTCT TGAGGAGCAG 
AATGAGGACA AAACCAAAAC AGCTAAGAAA AGGAAGAAAG AAGAAGAGAC CCCAAAAGAA 
AGCCTCGCAG AGGCTGAAGA CGTAAAACAG AAGGAAGAAA AGGAGGGGGA CCAGCTCATA 
GTCCCCTCTG AGCCCACAAA GTCCCCTGAG TCAGTAACCC TGACAAAGAC AGAGAACATT 
CCAGTGTGTA AGGCAGGAGT GAAACTGAAG CCTCAGGAAG AGGAACAGAG CAAACCTCCT 
GCTAGAGGTG CCAAGACACT GAGCAGTTTC TTCACACCCC GAAAACCAGC AGTGAAAACT 
GAAGTCAAAC AAGAAGAGTC AGGCACTCTA AGGAAGGAAG AGACCAAGGG AACCCTGGAT 
CCAGCAAATT ATAATCCTTC CAAGAATAAC TATCATCCCA TTGAAGATGC CTGCTGGAAA 
CATGGCCAGA AGGTCCCTTT TCTCGCCGTG GCCCGGACAT TTGAGAAGAT TGAGGAGGTT 
TCTGCTAGGC TCAAGATGGT GGAGACATTG AGCAACCTGC TGCGCTCCGT GGTGGCCCTG 
TCCCCTCCAG ACTTGCTCCC TGTTCTCTAT CTCAGCCTCA ACCGCCTCGG GCCACCTCAG 
CAGGGACTAG AGCTGGGTGT TGGTGATGGT GTCCTCCTCA AGGCCGTTGC CCAGGCCACA 
GGTCGTCAGC TGGAGTCCAT CCGGGCTGAG GTAGCTGAGA AGGGGGATGT GGGGCTGGTG 
GCCGAGAACA GCCGCAGCAC TCAGAGACTC ATGCTGCCTC CACCTCCACT TACTATTTCT 
GGAGTCTTCA CCAAATTCTG TGACATTGCC CGGCTCACTG GCAGTGCTTC CATGGCCAAG 
AAGATGGACA TTATCAAGGG CCTTTTTGTT GCCTGCCGCC ACTCAGAAGC CAGGTACATT 
GCCAGGTCCC TAAGCGGGCG GCTGCGCCTT GGCCTAGCTG AGCAGTCGGT ACTGGCTGCT 
CTTGCCCAGG CTGTGAGCCT CACACCCCCT GGCCAAGAAT TTCCCACGGC GGTGGTGGAT 
GCTGGGAAGG GCAAGACAGC AGAGGCCAGA AAGATGTGGT TGGAAGAACA AGGCATGATC 
TTGAAGCAGA CCTTCTGTGA GGTTCCTGAC CTGGATCGAA TCATCCCTGT GCTGCTGGAA 
CATGGCCTGG AACGTCTCCC AGAGCACTGC AAGCTGAGCC CAGGGGTCCC TCTTAAACCA 
ATGCTGGCTC ATCCCACTCG GGGTGTCAGT GAGGTCCTAA AACGCTTTGA GGAGGTCGAC 
TTCACCTGTG AATATAAATA CGATGGGCAG CGAGCACAGA TACATGTTCT GGAAGGTGGA 
GAAGTGAAGA TCTTCAGCAG GAACCAGGAA GACAACACAG GAAAGTATCC TGACATTATC 
AGCCGCATCC CCAAGATTAA ACACCCCTCA GTCACATCTT TCATTCTGGA CACTGAGGCT 
GTGGCCTGGG ACCGGGAAAA GAAGCAGATC CAACCATTCC AAGTACTCAC CACGCGCAAG 
CGCAAGGAGG TTGATGCATC GGAGATACAG GTGCAAGTGT GTCTGTATGC TTTTGACCTC 
ATCTACCTCA ACGGAGAGTC CCTGGTTCGC CAGCCCCTGT CTCGACGCCG GCAGCTGCTC 
CGTGAGAACT TCGTGGAGAC AGAGGGTGAG TTTGTCTTCA CCACCTCTTT GGACACCAAG 
GACACCGAGC AGATTGCTGA GTTCTTGGAG CAGTCAGTGA AGGACTCCTG TGAGGGCCTG 
ATGGTGAAGA CCTTGGATGT TGATGCCACT TATGAGATTG CCAAGAGGTC ACACAACTGG 
CTCAAGCTGA AGAAGGACTA CCTTGACGGT GTGGGTGACA CTCTGGACCT TGTGGTGATT 
GGTGCCTACC TGGGCCGGGG GAAGCGGGCT GGCCGGTATG GAGGCTTCCT GTTGGCTGCC 
TACGATGAGG AGAGTGAAGA GCTTCAGGCT ATATGCAAGC TGGGAACTGG ATTCAGCGAT 
GAGGAGCTGG AGGAACACCA CCAGAGCCTG CAGGCCCTGG TATTGCCTAC CCCACGCCCC 
TATGTGAGGA TTGATGGGGC AGTGGCCCCA GACCATTGGC TGGACCCAAG CATTGTGTGG 
GAGGTGAAGT GTGCTGATCT TTCCCTGTCC CCCATCTACC CTGCTGCACG GGGCCTGGTG 
GACAAAGAAA AAGGGATCTC TCTTCGTTTT CCTCGGTTCA TTCGTGTCCG TAAAGACAAG 
CAGCCAGAGC AGGCCACTAC CAGTAACCAG GTTGCCTCTT TGTACCGGAA GCAGAGTCAG 
ATTCAGAACC AACAAAGCTC AGACTTGGAC TCCGACGTTG AAGACTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076657.2
CDS	267..3017
Translation	MQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLDSDVEDY

Target ORF information:

RefSeq Version	NM_001083188.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083188.2

ORF Insert Sequence:

ATGCAGAGAA GTATTATGTC ATTTTTTCAA CCCACAAAAG AAGGTAAAGC GAAGAAGCCA 
GAGAAGGAGA CACCCAGCAG CATCAGAGAG AAGGAACCCC CTCCAAAGGT GGCGCTGAAG 
GAGAGGAATC AAGTGGTGCC CGAGAGTGAT TCTCCAGTGA AGAGGACAGG AAGGAAGGTA 
GCCCAGGTTC TGAGCTGTGA AGGGGAGGAC GAAGATGAAG CCCCTGGCAC CCCCAAAGTC 
CAGAAGCCTG TGTCAGACTC TGAACAGAGC TCTCCTCCCA GCCCTGACAC ATGTCCTGAG 
AACAGTCCTG TCTTCAACTG CAGCTCCCCC ATGGACATCT CCCCATCAGG ATTCCCAAAG 
CGTCGAACTG CGCGGAAGCA GCTCCCCAAA CGGACAATTC AAGACACTCT TGAGGAGCAG 
AATGAGGACA AAACCAAAAC AGCTAAGAAA AGGAAGAAAG AAGAAGAGAC CCCAAAAGAA 
AGCCTCGCAG AGGCTGAAGA CGTAAAACAG AAGGAAGAAA AGGAGGGGGA CCAGCTCATA 
GTCCCCTCTG AGCCCACAAA GTCCCCTGAG TCAGTAACCC TGACAAAGAC AGAGAACATT 
CCAGTGTGTA AGGCAGGAGT GAAACTGAAG CCTCAGGAAG AGGAACAGAG CAAACCTCCT 
GCTAGAGGTG CCAAGACACT GAGCAGTTTC TTCACACCCC GAAAACCAGC AGTGAAAACT 
GAAGTCAAAC AAGAAGAGTC AGGCACTCTA AGGAAGGAAG AGACCAAGGG AACCCTGGAT 
CCAGCAAATT ATAATCCTTC CAAGAATAAC TATCATCCCA TTGAAGATGC CTGCTGGAAA 
CATGGCCAGA AGGTCCCTTT TCTCGCCGTG GCCCGGACAT TTGAGAAGAT TGAGGAGGTT 
TCTGCTAGGC TCAAGATGGT GGAGACATTG AGCAACCTGC TGCGCTCCGT GGTGGCCCTG 
TCCCCTCCAG ACTTGCTCCC TGTTCTCTAT CTCAGCCTCA ACCGCCTCGG GCCACCTCAG 
CAGGGACTAG AGCTGGGTGT TGGTGATGGT GTCCTCCTCA AGGCCGTTGC CCAGGCCACA 
GGTCGTCAGC TGGAGTCCAT CCGGGCTGAG GTAGCTGAGA AGGGGGATGT GGGGCTGGTG 
GCCGAGAACA GCCGCAGCAC TCAGAGACTC ATGCTGCCTC CACCTCCACT TACTATTTCT 
GGAGTCTTCA CCAAATTCTG TGACATTGCC CGGCTCACTG GCAGTGCTTC CATGGCCAAG 
AAGATGGACA TTATCAAGGG CCTTTTTGTT GCCTGCCGCC ACTCAGAAGC CAGGTACATT 
GCCAGGTCCC TAAGCGGGCG GCTGCGCCTT GGCCTAGCTG AGCAGTCGGT ACTGGCTGCT 
CTTGCCCAGG CTGTGAGCCT CACACCCCCT GGCCAAGAAT TTCCCACGGC GGTGGTGGAT 
GCTGGGAAGG GCAAGACAGC AGAGGCCAGA AAGATGTGGT TGGAAGAACA AGGCATGATC 
TTGAAGCAGA CCTTCTGTGA GGTTCCTGAC CTGGATCGAA TCATCCCTGT GCTGCTGGAA 
CATGGCCTGG AACGTCTCCC AGAGCACTGC AAGCTGAGCC CAGGGGTCCC TCTTAAACCA 
ATGCTGGCTC ATCCCACTCG GGGTGTCAGT GAGGTCCTAA AACGCTTTGA GGAGGTCGAC 
TTCACCTGTG AATATAAATA CGATGGGCAG CGAGCACAGA TACATGTTCT GGAAGGTGGA 
GAAGTGAAGA TCTTCAGCAG GAACCAGGAA GACAACACAG GAAAGTATCC TGACATTATC 
AGCCGCATCC CCAAGATTAA ACACCCCTCA GTCACATCTT TCATTCTGGA CACTGAGGCT 
GTGGCCTGGG ACCGGGAAAA GAAGCAGATC CAACCATTCC AAGTACTCAC CACGCGCAAG 
CGCAAGGAGG TTGATGCATC GGAGATACAG GTGCAAGTGT GTCTGTATGC TTTTGACCTC 
ATCTACCTCA ACGGAGAGTC CCTGGTTCGC CAGCCCCTGT CTCGACGCCG GCAGCTGCTC 
CGTGAGAACT TCGTGGAGAC AGAGGGTGAG TTTGTCTTCA CCACCTCTTT GGACACCAAG 
GACACCGAGC AGATTGCTGA GTTCTTGGAG CAGTCAGTGA AGGACTCCTG TGAGGGCCTG 
ATGGTGAAGA CCTTGGATGT TGATGCCACT TATGAGATTG CCAAGAGGTC ACACAACTGG 
CTCAAGCTGA AGAAGGACTA CCTTGACGGT GTGGGTGACA CTCTGGACCT TGTGGTGATT 
GGTGCCTACC TGGGCCGGGG GAAGCGGGCT GGCCGGTATG GAGGCTTCCT GTTGGCTGCC 
TACGATGAGG AGAGTGAAGA GCTTCAGGCT ATATGCAAGC TGGGAACTGG ATTCAGCGAT 
GAGGAGCTGG AGGAACACCA CCAGAGCCTG CAGGCCCTGG TATTGCCTAC CCCACGCCCC 
TATGTGAGGA TTGATGGGGC AGTGGCCCCA GACCATTGGC TGGACCCAAG CATTGTGTGG 
GAGGTGAAGT GTGCTGATCT TTCCCTGTCC CCCATCTACC CTGCTGCACG GGGCCTGGTG 
GACAAAGAAA AAGGGATCTC TCTTCGTTTT CCTCGGTTCA TTCGTGTCCG TAAAGACAAG 
CAGCCAGAGC AGGCCACTAC CAGTAACCAG GTTGCCTCTT TGTACCGGAA GCAGAGTCAG 
ATTCAGAACC AACAAAGCTC AGACTTGGAC TCCGACGTTG AAGACTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)
Zhou L,Dai H,Wu J,Zhou M,Yuan H,Du J,Yang L,Wu X,Xu H,Hua Y,Xu J,Zheng L,Shen B

LGI1 acts presynaptically to regulate excitatory synaptic transmission during early postnatal development.
Scientific reports621769(2016 Feb)
Boillot M,Lee CY,Allene C,Leguern E,Baulac S,Rouach N

DNA ligase I is not essential for mammalian cell viability.
Cell reports7(2)316-20(2014 Apr)
Han L,Masani S,Hsieh CL,Yu K

Genome-wide ENU mutagenesis in combination with high density SNP analysis and exome sequencing provides rapid identification of novel mouse models of developmental disease.
PloS one8(3)e55429(2013)
Caruana G,Farlie PG,Hart AH,Bagheri-Fam S,Wallace MJ,Dobbie MS,Gordon CT,Miller KA,Whittle B,Abud HE,Arkell RM,Cole TJ,Harley VR,Smyth IM,Bertram JF

The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos.
Cytogenetic and genome research139(2)71-9(2013)
Linke M,May A,Reifenberg K,Haaf T,Zechner U

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Lig1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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