Krt27 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Krt27
Entrez Gene ID	16675
Full Name	keratin 27
Synonyms	CK-27,Krt1-c29,mIRSa3.1,stpm
General protein information	Preferred Names keratin 27 Names keratin, type I cytoskeletal 27 cytokeratin-27 keratin complex-1, acidic, gene C29 keratin complex-1, gene C29 type I inner root sheath-specific keratin-K25irs3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 D\|11 62.92 cM
Summary	This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The mouse type I keratin genes are clustered in a region of chromosome 11. The encoded protein is involved in the formation of intermediate filaments in the inner root sheath. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010666.2	NP_034796.1	keratin, type I cytoskeletal 27

Pathways from BioSystems

Homologies

Homo sapiens (human)	KRT27	NP_853515.2
Pan troglodytes (chimpanzee)	KRT27	XP_523630.2
	LOC100430195	XP_002800554.1
Canis lupus familiaris (dog)	KRT27	XP_548127.1
Bos taurus (cattle)	KRT27	NP_001069283.1
Rattus norvegicus (Norway rat)	Krt27	NP_001008824.1
Xenopus tropicalis (tropical clawed frog)	krt16	XP_004918756.1
Mus musculus (house mouse)	Krt27	NP_034796.1

Gene Ontology
Bibliography

Related articles in PubMed

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Fairfield H, Srivastava A, Ananda G, Liu R, Kircher M, Lakshminarayana A, Harris BS, Karst SY, Dionne LA, Kane CC, Curtain M, Berry ML, Ward-Bailey PF, Greenstein I, Byers C, Czechanski A, Sharp J, Palmer K, Gudis P, Martin W, Tadenev A, Bogdanik L, Pratt CH, Chang B, Schroeder DG, Cox GA, Cliften P, Milbrandt J, Murray S, Burgess R, Bergstrom DE, Donahue LR, Hamamy H, Masri A, Santoni FA, Makrythanasis P, Antonarakis SE, Shendure J, Reinholdt LG
Genome research25(7)948-57(2015 Jul)

Mutations in the helix termination motif of mouse type I IRS keratin genes impair the assembly of keratin intermediate filament.
Tanaka S, Miura I, Yoshiki A, Kato Y, Yokoyama H, Shinogi A, Masuya H, Wakana S, Tamura M, Shiroishi T
Genomics90(6)703-11(2007 Dec)

K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) represent the type I inner root sheath keratins of the human hair follicle.
Langbein L, Rogers MA, Praetzel-Wunder S, Helmke B, Schirmacher P, Schweizer J
The Journal of investigative dermatology126(11)2377-86(2006 Nov)

New consensus nomenclature for mammalian keratins.
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW
The Journal of cell biology174(2)169-74(2006 Jul)

The dominant alopecia phenotypes Bareskin, Rex-denuded, and Reduced Coat 2 are caused by mutations in gasdermin 3.
Runkel F, Marquardt A, Stoeger C, Kochmann E, Simon D, Kohnke B, Korthaus D, Wattler F, Fuchs H, Hrab? de Angelis M, Stumm G, Nehls M, Wattler S, Franz T, Augustin M
Genomics84(5)824-35(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Krt27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Krt27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06571	NM_010666.2 Latest version!	Mus musculus keratin 27 (Krt27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Krt27 ( NM_010666.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034796.1 Mus musculus keratin 27 (Krt27), mRNA.

Price & Availability↑

CloneID	OMu06571
Clone ID Related Accession (Same CDS sequence)	NM_010666.2
Accession Version	NM_010666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	keratin, type I cytoskeletal 27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590991.14, AK077384.1 and AA756799.1. On Sep 16, 2015 this sequence version replaced NM_010666.1. ##Evidence-Data-START## Transcript exon combination :: BC140996.1, AK077384.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849383, SAMN00849384 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGTTC GCTTTTCTTC CGCATCCAGA CGGCTGGGCT CTGTGAGGCT CTCCAGCGCG 
GGAGCAGCCT TGGGGGCTGG AAACGCATGC GGAGTGCCGG GCATCGGAAG CGGCTTCTCC 
TGCGCCTTTG GGGGCAGTTC ATTGGCAGGG GGGCTCGGTA TGGGCGGTGC TTCTTGTGGG 
GCCTTCACAG CGAATGAGCA CGGCCTCCTC TCCGGCAATG AGAAGGTCAC CATGCAGAAC 
CTTAATGACC GCCTGGCCTC CTACCTGGAG AACGTGCAAG CACTGGAGGA GGCCAATGCT 
GACCTGGAGC AGAAGATCAA GGACTGGTAT GAAAAATTTG GACCTGGGTC GTGCCGCGGT 
CTCGATCATG ACTACAGTAG ATACTTCCCC ATCATTGATG ACCTCAGGAC CCAGATCATC 
TCTGCAACTG CACACAACGC CAACATCATC CTGCAAAATG ACAACGCAAG ACTGACGGCG 
GATGACTTCA GAATGAAGTA TGAGAATGAA CTGGCACTCC ACCAGAGCGT CGATGCTGAC 
ATCAATGGCT TGCGGAGGGT CCTGGATGAG CTGACCCTGT GCAGAACCGA CCTTGAGGTC 
CAGCTGGAAA CACTCAGTGA GGAGCTGGCA TACCTCAAGA AGAACCACGA GGAGGAAATG 
CAGGCTCTGC AGTGCGCCGC TGGAGGCAAC GTGAACGTGG AGATGAACGC AGCCCCGGGC 
GTGGACCTCA CCGTCCTGCT GAACAACATG AGAGCCGAGT ACGAGGCCCT GGCTGAGCAG 
AACCGTAGGG ACGCCGAGGC CTGGTTCCAA GAGAAGAGCG CCTCCCTGCA GCAGCAGATT 
TCAGACGATG CTGGAGCCAC CACCTCAGCC AGGAACGAAC TTACAGAGAT GAAACGGACT 
CTCCAAACCC TGGAGATAGA GCTACAGTCC CTCTTAGCCA TGAAACACTC TCTGGAGTGC 
TCCCTGACGG AGACCGAGGG CAACTACTGC ACGCAGCTGG CTCAGATCCA GGCTCAGATC 
AGCGCCCTGG AGGAGCAGCT GCACCAAGTG AGGACGGAGA CGGAGGGCCA GAAGCTGGAA 
TATGAGCAGC TGCTGAATGT CAAGGCCCAC CTGGAGAAGG AGATCGAGAC CTACTGCCGC 
CTCATAGATG GAGACGAGGG CTCTTGTGTT AAGGCAAAAG GCCAAGGAAG ACCCGGAAAT 
CAGACAAAAG ATTCGCCTAA AACTGCCATC GTTAAAACTG TCGTGGAAGA GTTAGACCCC 
CGTGGCAAGG TTCTCTCCTC CAGAGTTCAC ACCCTGGAAG AGAAATCCAC CAAAGTCAAC 
AAGACCGAGC AGAGGATCCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034796.1
CDS	46..1392
Translation	MSVRFSSASRRLGSVRLSSAGAALGAGNACGVPGIGSGFSCAFGGSSLAGGLGMGGASCGAFTANEHGLLSGNEKVTMQNLNDRLASYLENVQALEEANADLEQKIKDWYEKFGPGSCRGLDHDYSRYFPIIDDLRTQIISATAHNANIILQNDNARLTADDFRMKYENELALHQSVDADINGLRRVLDELTLCRTDLEVQLETLSEELAYLKKNHEEEMQALQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQEKSASLQQQISDDAGATTSARNELTEMKRTLQTLEIELQSLLAMKHSLECSLTETEGNYCTQLAQIQAQISALEEQLHQVRTETEGQKLEYEQLLNVKAHLEKEIETYCRLIDGDEGSCVKAKGQGRPGNQTKDSPKTAIVKTVVEELDPRGKVLSSRVHTLEEKSTKVNKTEQRIPS

Target ORF information:

RefSeq Version	NM_010666.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus keratin 27 (Krt27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010666.2

ORF Insert Sequence:

ATGTCCGTTC GCTTTTCTTC CGCATCCAGA CGGCTGGGCT CTGTGAGGCT CTCCAGCGCG 
GGAGCAGCCT TGGGGGCTGG AAACGCATGC GGAGTGCCGG GCATCGGAAG CGGCTTCTCC 
TGCGCCTTTG GGGGCAGTTC ATTGGCAGGG GGGCTCGGTA TGGGCGGTGC TTCTTGTGGG 
GCCTTCACAG CGAATGAGCA CGGCCTCCTC TCCGGCAATG AGAAGGTCAC CATGCAGAAC 
CTTAATGACC GCCTGGCCTC CTACCTGGAG AACGTGCAAG CACTGGAGGA GGCCAATGCT 
GACCTGGAGC AGAAGATCAA GGACTGGTAT GAAAAATTTG GACCTGGGTC GTGCCGCGGT 
CTCGATCATG ACTACAGTAG ATACTTCCCC ATCATTGATG ACCTCAGGAC CCAGATCATC 
TCTGCAACTG CACACAACGC CAACATCATC CTGCAAAATG ACAACGCAAG ACTGACGGCG 
GATGACTTCA GAATGAAGTA TGAGAATGAA CTGGCACTCC ACCAGAGCGT CGATGCTGAC 
ATCAATGGCT TGCGGAGGGT CCTGGATGAG CTGACCCTGT GCAGAACCGA CCTTGAGGTC 
CAGCTGGAAA CACTCAGTGA GGAGCTGGCA TACCTCAAGA AGAACCACGA GGAGGAAATG 
CAGGCTCTGC AGTGCGCCGC TGGAGGCAAC GTGAACGTGG AGATGAACGC AGCCCCGGGC 
GTGGACCTCA CCGTCCTGCT GAACAACATG AGAGCCGAGT ACGAGGCCCT GGCTGAGCAG 
AACCGTAGGG ACGCCGAGGC CTGGTTCCAA GAGAAGAGCG CCTCCCTGCA GCAGCAGATT 
TCAGACGATG CTGGAGCCAC CACCTCAGCC AGGAACGAAC TTACAGAGAT GAAACGGACT 
CTCCAAACCC TGGAGATAGA GCTACAGTCC CTCTTAGCCA TGAAACACTC TCTGGAGTGC 
TCCCTGACGG AGACCGAGGG CAACTACTGC ACGCAGCTGG CTCAGATCCA GGCTCAGATC 
AGCGCCCTGG AGGAGCAGCT GCACCAAGTG AGGACGGAGA CGGAGGGCCA GAAGCTGGAA 
TATGAGCAGC TGCTGAATGT CAAGGCCCAC CTGGAGAAGG AGATCGAGAC CTACTGCCGC 
CTCATAGATG GAGACGAGGG CTCTTGTGTT AAGGCAAAAG GCCAAGGAAG ACCCGGAAAT 
CAGACAAAAG ATTCGCCTAA AACTGCCATC GTTAAAACTG TCGTGGAAGA GTTAGACCCC 
CGTGGCAAGG TTCTCTCCTC CAGAGTTCAC ACCCTGGAAG AGAAATCCAC CAAAGTCAAC 
AAGACCGAGC AGAGGATCCC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Genome research25(7)948-57(2015 Jul)
Fairfield H,Srivastava A,Ananda G,Liu R,Kircher M,Lakshminarayana A,Harris BS,Karst SY,Dionne LA,Kane CC,Curtain M,Berry ML,Ward-Bailey PF,Greenstein I,Byers C,Czechanski A,Sharp J,Palmer K,Gudis P,Martin W,Tadenev A,Bogdanik L,Pratt CH,Chang B,Schroeder DG,Cox GA,Cliften P,Milbrandt J,Murray S,Burgess R,Bergstrom DE,Donahue LR,Hamamy H,Masri A,Santoni FA,Makrythanasis P,Antonarakis SE,Shendure J,Reinholdt LG

Mutations in the helix termination motif of mouse type I IRS keratin genes impair the assembly of keratin intermediate filament.
Genomics90(6)703-11(2007 Dec)
Tanaka S,Miura I,Yoshiki A,Kato Y,Yokoyama H,Shinogi A,Masuya H,Wakana S,Tamura M,Shiroishi T

K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) represent the type I inner root sheath keratins of the human hair follicle.
The Journal of investigative dermatology126(11)2377-86(2006 Nov)
Langbein L,Rogers MA,Praetzel-Wunder S,Helmke B,Schirmacher P,Schweizer J

New consensus nomenclature for mammalian keratins.
The Journal of cell biology174(2)169-74(2006 Jul)
Schweizer J,Bowden PE,Coulombe PA,Langbein L,Lane EB,Magin TM,Maltais L,Omary MB,Parry DA,Rogers MA,Wright MW

The dominant alopecia phenotypes Bareskin, Rex-denuded, and Reduced Coat 2 are caused by mutations in gasdermin 3.
Genomics84(5)824-35(2004 Nov)
Runkel F,Marquardt A,Stoeger C,Kochmann E,Simon D,Kohnke B,Korthaus D,Wattler F,Fuchs H,Hrab? de Angelis M,Stumm G,Nehls M,Wattler S,Franz T,Augustin M

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Krt27 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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