Mdfic cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mdfic
Entrez Gene ID	16543
Full Name	MyoD family inhibitor domain containing
Synonyms	Kdt1,Mdfid
General protein information	Preferred Names MyoD family inhibitor domain containing Names myoD family inhibitor domain-containing protein I-mfa domain-containing protein kidney cell line-derived transcript 1 mdfic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 A1
Summary	This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, which in human have been shown to have different subcellular localization. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030255200.1	XP_030111060.1	myoD family inhibitor domain-containing protein isoform X1
XM_017321412.1	XP_017176901.1	myoD family inhibitor domain-containing protein isoform X1
NM_175088.5	NP_780297.3	myoD family inhibitor domain-containing protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MDFIC	NP_001094572.1
Danio rerio (zebrafish)	si:ch211-262n1.4	XP_001919127.2
Mus musculus (house mouse)	Mdfic	NP_780297.3
Gallus gallus (chicken)	MDFIC	XP_416018.1
Danio rerio (zebrafish)	si:ch73-156e19.1	XP_003198654.1
Rattus norvegicus (Norway rat)	Mdfic	NP_001099138.1
Homo sapiens (human)	MDFIC	NP_001159817.1
Pan troglodytes (chimpanzee)	MDFIC	XP_001137214.2
Canis lupus familiaris (dog)	LOC612042	XP_005628805.1
Xenopus tropicalis (tropical clawed frog)	mdfic	XP_002932863.1

Gene Ontology
Bibliography

Related articles in PubMed

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

Nucleolar release of Hand1 acts as a molecular switch to determine cell fate.
Martindill DM, Risebro CA, Smart N, Franco-Viseras Mdel M, Rosario CO, Swallow CJ, Dennis JW, Riley PR
Nature cell biology9(10)1131-41(2007 Oct)

Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family.
Ishiguro A, Inoue T, Mikoshiba K, Aruga J
Biochemical and biophysical research communications324(1)302-7(2004 Nov)

Myogenic repressor I-mfa interferes with the function of Zic family proteins.
Mizugishi K, Hatayama M, Tohmonda T, Ogawa M, Inoue T, Mikoshiba K, Aruga J
Biochemical and biophysical research communications320(1)233-40(2004 Jul)

Molecular cloning of a novel human I-mfa domain-containing protein that differently regulates human T-cell leukemia virus type I and HIV-1 expression.
Th?bault S, Gachon F, Lemasson I, Devaux C, Mesnard JM
The Journal of biological chemistry275(7)4848-57(2000 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mdfic gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mdfic cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu81655	XM_017321412.1 Latest version!	Mus musculus MyoD family inhibitor domain containing (Mdfic), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu81655	XM_030255200.1 Latest version!	Mus musculus MyoD family inhibitor domain containing (Mdfic), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu04837	NM_175088.5 Latest version!	Mus musculus MyoD family inhibitor domain containing (Mdfic), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mdfic ( XM_030255200.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111060.1 Mus musculus MyoD family inhibitor domain containing (Mdfic), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81655
Clone ID Related Accession (Same CDS sequence)	XM_030255200.1 , XM_017321412.1
Accession Version	XM_030255200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myoD family inhibitor domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGACC AGTCCATTTG GCCAAATCCT TCAGCTGGTG AACTTGTCAG AACCCAACCT 
GAACGTCTGC CTCAACTCCA GACATCAGCA CAGGAACCCG GCAAGGAGGA AACAGGCAAG 
ATAAAGAATG GCGGCCACAC AAGGATGAGC AATGGGAATG GAATTCCTCA CGGAGCCAAA 
CATGTGTCCG TGGAGAATCA CAAGATATCA GCCCCCGTTT CTCAGAAGAT GCACAGAAAA 
ATCCAGTCCA GCTTGTCTGT AAACAATGAC ATCAGTAAGA AGAGTAAAGT AAATGCTGTC 
TTTTCCCCAA AAGCAGCCTC CTCACCCGAA GACTGCTGTG TCCACTGTAT CCTGGCATGC 
TTGTTCTGTG AATTCCTGAC CCTCTGCAAC ATTGTCCTGG GACAAGCTTC GTGTGGCATC 
TGCACCTCCG AAGCCTGCTG CTGTTGCTGT GGCGATGAGA TGGGTGATGA CTGTAGCTGC 
CCCTGTGACA TGGACTGCGG CATCATGGAT GCCTGTTGTG AATCGTCAGA CTGTCTAGAA 
ATCTGCATGG AATGCTGTGG CATCTGTTTT CCTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111060.1
CDS	982..1560
Translation	MRDQSIWPNPSAGELVRTQPERLPQLQTSAQEPGKEETGKIKNGGHTRMSNGNGIPHGAKHVSVENHKISAPVSQKMHRKIQSSLSVNNDISKKSKVNAVFSPKAASSPEDCCVHCILACLFCEFLTLCNIVLGQASCGICTSEACCCCCGDEMGDDCSCPCDMDCGIMDACCESSDCLEICMECCGICFPS

Target ORF information:

RefSeq Version	XM_030255200.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MyoD family inhibitor domain containing (Mdfic), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255200.1

ORF Insert Sequence:

ATGCGGGACC AGTCCATTTG GCCAAATCCT TCAGCTGGTG AACTTGTCAG AACCCAACCT 
GAACGTCTGC CTCAACTCCA GACATCAGCA CAGGAACCCG GCAAGGAGGA AACAGGCAAG 
ATAAAGAATG GCGGCCACAC AAGGATGAGC AATGGGAATG GAATTCCTCA CGGAGCCAAA 
CATGTGTCCG TGGAGAATCA CAAGATATCA GCCCCCGTTT CTCAGAAGAT GCACAGAAAA 
ATCCAGTCCA GCTTGTCTGT AAACAATGAC ATCAGTAAGA AGAGTAAAGT AAATGCTGTC 
TTTTCCCCAA AAGCAGCCTC CTCACCCGAA GACTGCTGTG TCCACTGTAT CCTGGCATGC 
TTGTTCTGTG AATTCCTGAC CCTCTGCAAC ATTGTCCTGG GACAAGCTTC GTGTGGCATC 
TGCACCTCCG AAGCCTGCTG CTGTTGCTGT GGCGATGAGA TGGGTGATGA CTGTAGCTGC 
CCCTGTGACA TGGACTGCGG CATCATGGAT GCCTGTTGTG AATCGTCAGA CTGTCTAGAA 
ATCTGCATGG AATGCTGTGG CATCTGTTTT CCTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdfic ( XM_017321412.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176901.1 Mus musculus MyoD family inhibitor domain containing (Mdfic), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81655
Clone ID Related Accession (Same CDS sequence)	XM_030255200.1 , XM_017321412.1
Accession Version	XM_017321412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myoD family inhibitor domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGACC AGTCCATTTG GCCAAATCCT TCAGCTGGTG AACTTGTCAG AACCCAACCT 
GAACGTCTGC CTCAACTCCA GACATCAGCA CAGGAACCCG GCAAGGAGGA AACAGGCAAG 
ATAAAGAATG GCGGCCACAC AAGGATGAGC AATGGGAATG GAATTCCTCA CGGAGCCAAA 
CATGTGTCCG TGGAGAATCA CAAGATATCA GCCCCCGTTT CTCAGAAGAT GCACAGAAAA 
ATCCAGTCCA GCTTGTCTGT AAACAATGAC ATCAGTAAGA AGAGTAAAGT AAATGCTGTC 
TTTTCCCCAA AAGCAGCCTC CTCACCCGAA GACTGCTGTG TCCACTGTAT CCTGGCATGC 
TTGTTCTGTG AATTCCTGAC CCTCTGCAAC ATTGTCCTGG GACAAGCTTC GTGTGGCATC 
TGCACCTCCG AAGCCTGCTG CTGTTGCTGT GGCGATGAGA TGGGTGATGA CTGTAGCTGC 
CCCTGTGACA TGGACTGCGG CATCATGGAT GCCTGTTGTG AATCGTCAGA CTGTCTAGAA 
ATCTGCATGG AATGCTGTGG CATCTGTTTT CCTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176901.1
CDS	495..1073
Translation	MRDQSIWPNPSAGELVRTQPERLPQLQTSAQEPGKEETGKIKNGGHTRMSNGNGIPHGAKHVSVENHKISAPVSQKMHRKIQSSLSVNNDISKKSKVNAVFSPKAASSPEDCCVHCILACLFCEFLTLCNIVLGQASCGICTSEACCCCCGDEMGDDCSCPCDMDCGIMDACCESSDCLEICMECCGICFPS

Target ORF information:

RefSeq Version	XM_017321412.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MyoD family inhibitor domain containing (Mdfic), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321412.1

ORF Insert Sequence:

ATGCGGGACC AGTCCATTTG GCCAAATCCT TCAGCTGGTG AACTTGTCAG AACCCAACCT 
GAACGTCTGC CTCAACTCCA GACATCAGCA CAGGAACCCG GCAAGGAGGA AACAGGCAAG 
ATAAAGAATG GCGGCCACAC AAGGATGAGC AATGGGAATG GAATTCCTCA CGGAGCCAAA 
CATGTGTCCG TGGAGAATCA CAAGATATCA GCCCCCGTTT CTCAGAAGAT GCACAGAAAA 
ATCCAGTCCA GCTTGTCTGT AAACAATGAC ATCAGTAAGA AGAGTAAAGT AAATGCTGTC 
TTTTCCCCAA AAGCAGCCTC CTCACCCGAA GACTGCTGTG TCCACTGTAT CCTGGCATGC 
TTGTTCTGTG AATTCCTGAC CCTCTGCAAC ATTGTCCTGG GACAAGCTTC GTGTGGCATC 
TGCACCTCCG AAGCCTGCTG CTGTTGCTGT GGCGATGAGA TGGGTGATGA CTGTAGCTGC 
CCCTGTGACA TGGACTGCGG CATCATGGAT GCCTGTTGTG AATCGTCAGA CTGTCTAGAA 
ATCTGCATGG AATGCTGTGG CATCTGTTTT CCTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdfic ( NM_175088.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780297.3 Mus musculus MyoD family inhibitor domain containing (Mdfic), mRNA.

Price & Availability↑

CloneID	OMu04837
Clone ID Related Accession (Same CDS sequence)	NM_175088.5
Accession Version	NM_175088.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	myoD family inhibitor domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK165016.1, BB603899.2, BG083307.2, AK087298.1, CO043232.1 and BY509244.1. On Apr 9, 2008 this sequence version replaced NM_175088.4. Sequence Note: An AUG translation start codon is selected for this RefSeq based on its conservation with the homologous human isoform, p32. Studies in PMID:10671520 demonstrate the existence of a longer human isoform, p40, which can initiate translation from an upstream non-AUG (GUG) start codon. Possible in-frame, upstream GUG start codons are also present in mouse, which could extend the protein N-terminal by 82, 106 or 117 aa; however, these extensions are not conserved with the human p40 N-terminal. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.50847.1, AK165016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGCG CGGGTGAAGC CCTCGCTCCC GGGCCCGCGG AGCAGCAGTG CCCGGTCGAG 
GCGGGCGGCG GCCGGCTGGG CTCCCCGGCC CACGAAGCAT GTAATGAGGA CAATACGGAG 
AAAGATAAAA GGCCAGCTAC CAGTGGCCAT ACCAGGTGTG GGCTGATGCG GGACCAGTCC 
ATTTGGCCAA ATCCTTCAGC TGGTGAACTT GTCAGAACCC AACCTGAACG TCTGCCTCAA 
CTCCAGACAT CAGCACAGGA ACCCGGCAAG GAGGAAACAG GCAAGATAAA GAATGGCGGC 
CACACAAGGA TGAGCAATGG GAATGGAATT CCTCACGGAG CCAAACATGT GTCCGTGGAG 
AATCACAAGA TATCAGCCCC CGTTTCTCAG AAGATGCACA GAAAAATCCA GTCCAGCTTG 
TCTGTAAACA ATGACATCAG TAAGAAGAGT AAAGTAAATG CTGTCTTTTC CCCAAAAGCA 
GCCTCCTCAC CCGAAGACTG CTGTGTCCAC TGTATCCTGG CATGCTTGTT CTGTGAATTC 
CTGACCCTCT GCAACATTGT CCTGGGACAA GCTTCGTGTG GCATCTGCAC CTCCGAAGCC 
TGCTGCTGTT GCTGTGGCGA TGAGATGGGT GATGACTGTA GCTGCCCCTG TGACATGGAC 
TGCGGCATCA TGGATGCCTG TTGTGAATCG TCAGACTGTC TAGAAATCTG CATGGAATGC 
TGTGGCATCT GTTTTCCTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780297.3
CDS	388..1131
Translation	MSCAGEALAPGPAEQQCPVEAGGGRLGSPAHEACNEDNTEKDKRPATSGHTRCGLMRDQSIWPNPSAGELVRTQPERLPQLQTSAQEPGKEETGKIKNGGHTRMSNGNGIPHGAKHVSVENHKISAPVSQKMHRKIQSSLSVNNDISKKSKVNAVFSPKAASSPEDCCVHCILACLFCEFLTLCNIVLGQASCGICTSEACCCCCGDEMGDDCSCPCDMDCGIMDACCESSDCLEICMECCGICFPS

Target ORF information:

RefSeq Version	NM_175088.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus MyoD family inhibitor domain containing (Mdfic), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175088.5

ORF Insert Sequence:

ATGTCCTGCG CGGGTGAAGC CCTCGCTCCC GGGCCCGCGG AGCAGCAGTG CCCGGTCGAG 
GCGGGCGGCG GCCGGCTGGG CTCCCCGGCC CACGAAGCAT GTAATGAGGA CAATACGGAG 
AAAGATAAAA GGCCAGCTAC CAGTGGCCAT ACCAGGTGTG GGCTGATGCG GGACCAGTCC 
ATTTGGCCAA ATCCTTCAGC TGGTGAACTT GTCAGAACCC AACCTGAACG TCTGCCTCAA 
CTCCAGACAT CAGCACAGGA ACCCGGCAAG GAGGAAACAG GCAAGATAAA GAATGGCGGC 
CACACAAGGA TGAGCAATGG GAATGGAATT CCTCACGGAG CCAAACATGT GTCCGTGGAG 
AATCACAAGA TATCAGCCCC CGTTTCTCAG AAGATGCACA GAAAAATCCA GTCCAGCTTG 
TCTGTAAACA ATGACATCAG TAAGAAGAGT AAAGTAAATG CTGTCTTTTC CCCAAAAGCA 
GCCTCCTCAC CCGAAGACTG CTGTGTCCAC TGTATCCTGG CATGCTTGTT CTGTGAATTC 
CTGACCCTCT GCAACATTGT CCTGGGACAA GCTTCGTGTG GCATCTGCAC CTCCGAAGCC 
TGCTGCTGTT GCTGTGGCGA TGAGATGGGT GATGACTGTA GCTGCCCCTG TGACATGGAC 
TGCGGCATCA TGGATGCCTG TTGTGAATCG TCAGACTGTC TAGAAATCTG CATGGAATGC 
TGTGGCATCT GTTTTCCTTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

Nucleolar release of Hand1 acts as a molecular switch to determine cell fate.
Nature cell biology9(10)1131-41(2007 Oct)
Martindill DM,Risebro CA,Smart N,Franco-Viseras Mdel M,Rosario CO,Swallow CJ,Dennis JW,Riley PR

Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family.
Biochemical and biophysical research communications324(1)302-7(2004 Nov)
Ishiguro A,Inoue T,Mikoshiba K,Aruga J

Myogenic repressor I-mfa interferes with the function of Zic family proteins.
Biochemical and biophysical research communications320(1)233-40(2004 Jul)
Mizugishi K,Hatayama M,Tohmonda T,Ogawa M,Inoue T,Mikoshiba K,Aruga J

Molecular cloning of a novel human I-mfa domain-containing protein that differently regulates human T-cell leukemia virus type I and HIV-1 expression.
The Journal of biological chemistry275(7)4848-57(2000 Feb)
Th?bault S,Gachon F,Lemasson I,Devaux C,Mesnard JM

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Mdfic cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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