Ajuba cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ajuba
Entrez Gene ID	16475
Full Name	ajuba LIM protein
Synonyms	Jub
General protein information	Preferred Names ajuba LIM protein Names LIM domain-containing protein ajuba protein ajuba
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_010590.5	NP_034720.2	LIM domain-containing protein ajuba

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AJUBA	NP_001179446.1
Homo sapiens (human)	AJUBA	NP_116265.1
	JUB	XP_001104222.1
Canis lupus familiaris (dog)	AJUBA	XP_005623277.1
Mus musculus (house mouse)	Ajuba	NP_034720.2
Rattus norvegicus (Norway rat)	Ajuba	NP_445955.1

Gene Ontology
Bibliography

Related articles in PubMed

p63 exerts spatio-temporal control of palatal epithelial cell fate to prevent cleft palate.
Richardson R, Mitchell K, Hammond NL, Mollo MR, Kouwenhoven EN, Wyatt ND, Donaldson IJ, Zeef L, Burgis T, Blance R, van Heeringen SJ, Stunnenberg HG, Zhou H, Missero C, Romano RA, Sinha S, Dixon MJ, Dixon J
PLoS genetics13(6)e1006828(2017 Jun)

The LIM protein Ajuba promotes adipogenesis by enhancing PPAR? and p300/CBP interaction.
Li Q, Peng H, Fan H, Zou X, Liu Q, Zhang Y, Xu H, Chu Y, Wang C, Ayyanathan K, Rauscher FJ, Zhang K, Hou Z
Cell death and differentiation23(1)158-68(2016 Jan)

Ajuba Preferentially Binds LXR?/RXR? Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.
Fan H, Dong W, Li Q, Zou X, Zhang Y, Wang J, Li S, Liu W, Dong Y, Sun H, Hou Z
Molecular endocrinology (Baltimore, Md.)29(11)1608-18(2015 Nov)

A conserved MST1/2-YAP axis mediates Hippo signaling during lung growth.
Lin C, Yao E, Chuang PT
Developmental biology403(1)101-13(2015 Jul)

Adhesion molecule-mediated hippo pathway modulates hemangioendothelioma cell behavior.
Tsuneki M, Madri JA
Molecular and cellular biology34(24)4485-99(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ajuba recruits p300/CBP via its LIM domain and facilitates p300/CBP binding to PPARg. Moreover, Ajuba, PPARg, p300/CBP can cooperatively occupy the PPARg target promoters and concomitantly increases histone acetylation at these loci.
Title: The LIM protein Ajuba promotes adipogenesis by enhancing PPAR? and p300/CBP interaction.

Ajuba is a novel coactivator for liver X receptors and may play important role in lipid and glucose metabolism.
Title: Ajuba Preferentially Binds LXR?/RXR? Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.

Findings support the importance of adhesion molecules (VE-cadherin and CD31), survivin, and Ajuba in modulating the Hippo pathway, which regulates, in part, proliferation and survival in hemangioendotheliomas.
Title: Adhesion molecule-mediated hippo pathway modulates hemangioendothelioma cell behavior.

PKD1-mediated phosphorylation of SNAI1 occurs in the nucleus and generates a nuclear, inactive DNA/SNAI1 complex that shows decreased interaction with its co-repressor Ajuba.
Title: Protein kinase D1 maintains the epithelial phenotype by inducing a DNA-bound, inactive SNAI1 transcriptional repressor complex.

identification of the protein arginine methyltransferase 5 (PRMT5) as an effector recruited to SNAIL through an interaction with AJUBA that functions to repress the SNAIL target gene, E-cadherin
Title: The LIM protein AJUBA recruits protein arginine methyltransferase 5 to mediate SNAIL-dependent transcriptional repression.

The following Ajuba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ajuba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06412	NM_010590.5 Latest version!	Mus musculus ajuba LIM protein (Ajuba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ajuba ( NM_010590.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034720.2 Mus musculus ajuba LIM protein (Ajuba), mRNA.

Price & Availability↑

CloneID	OMu06412
Clone ID Related Accession (Same CDS sequence)	NM_010590.5
Accession Version	NM_010590.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	LIM domain-containing protein ajuba
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164433.4. On Mar 24, 2011 this sequence version replaced NM_010590.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011035.1, U79776.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACGGT TAGGGGAGAA AGCCAGTCGT CTGCTAGAAA AGTTGAGACT CTCGGACTCC 
GGCAGCGCCA AGTTCGGCCG CAGAAAGGGC GAAGCGAGCC GGTCTGGGTC TGATGGGACC 
CCCGGAGCAG GCAAGGGACG CTTAAGCGGG TTGGGGGGAC CTAGGAAGTC AGGACACCGT 
GGAGCGAATG GGGGGCCTGG AGATGAACCT TTGGAACCGG CCAGGGAGCA AGGGCCCCTG 
GACGCCGAGC GGAACGCACG CGGCTCCTTT GAAGCGCAGC GCTTCGAAGG GTCCTTTCCC 
GGGGGGCCGC CGCCCACTCG AGCCCTGCCT CTGCCTCTGT CGTCGCCTCC TGATTTTCGG 
CTGGAGACCA CGGCTCCAGC CCTTAGCCCT CGCTCCAGCT TCGCCAGTAG CTCGGCCAGC 
GATGCGAGCA AGCCATCTAG CCCCCGGGGC AGCCTGCTGC TGGACGGAGC CGGGGCCAGC 
GGAGCCGGAG GTAGCCGGCC GTGCAGCAAT CGGACCAGCG GCATCAGCAT GGGCTACGAC 
CAGCGCCACG GGAGCCCGCT GCCCGCGGGG CCGTGCCTAT TCGGCCTCCC GCTGACCACT 
GCTCCTGCGG GCTACCCTGG AGGGGCTCCG TCCGCCTACC CGGAGCTCCA CGCTGCCCTG 
GACCGACTAT GTGCCCATCG GTCCGTGGGA TTCGGCTGCC AGGAGAGCCG TCACTCGTAC 
CCCCCGGCCC TGGGCAGCCC CGGAGCTCTA ACCGGAGCCG TGGTGGGAAC AGCGGGTCCT 
TTGGAGAGAC GTGGGGCGCA ACCCGGACGA CACTCGGTTA CAGGCTACGG GGACTGCGCC 
GCGGGGGCCC GTTACCAGGA CGAGCTAACA GCATTGCTGC GTTTGACCGT GGCTACCGGT 
GGGCGAGAAG CGGGTGCTCG CGGGGAACCC TCGGGGATTG AGCCGTCGGG TCTGGAGGAG 
TCTCCTGGTC CCTTCGTTCC AGAGGCCTCC CGATCACGGA TACGGGAGCC AGAGGCCAGA 
GAAGATTACT TTGGCACCTG TATCAAGTGC AACAAAGGTA TCTATGGGCA GAGCAATGCC 
TGCCAGGCCC TGGACAGCCT CTACCACACC CAGTGCTTTG TCTGCTGCTC CTGTGGACGA 
ACTTTGCGGT GCAAGGCTTT CTACAGCGTC AATGGCTCTG TCTACTGTGA GGAAGACTAT 
CTGTTTTCAG GGTTTCAGGA GGCAGCTGAG AAGTGCTGTG TCTGCGGCCA CTTGATTCTA 
GAGAAGATCC TCCAGGCCAT GGGGAAGTCC TATCACCCGG GCTGCTTCCG ATGCATCGTA 
TGTAACAAGT GCCTGGACGG CGTCCCCTTC ACTGTGGACT TCTCCAACCA GGTGTACTGT 
GTTACCGACT ACCACAAAAA TTACGCCCCG AAATGTGCAG CCTGCGGACA ACCCATCCTC 
CCCTCAGAGG GCTGTGAGGA CATTGTGAGG GTGATATCCA TGGACCGTGA TTATCACTTT 
GAGTGCTACC ACTGTGAGGA CTGCCGCATG CAGCTGAGTG ACGAGGAAGG TTGCTGCTGT 
TTCCCTCTTG ATGGACATTT GCTCTGCCAC GGCTGTCACA TGCAGCGCCT CAGTGCCCGG 
CAGCCCTCTA CCAACTATAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034720.2
CDS	398..2041
Translation	MERLGEKASRLLEKLRLSDSGSAKFGRRKGEASRSGSDGTPGAGKGRLSGLGGPRKSGHRGANGGPGDEPLEPAREQGPLDAERNARGSFEAQRFEGSFPGGPPPTRALPLPLSSPPDFRLETTAPALSPRSSFASSSASDASKPSSPRGSLLLDGAGASGAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGLPLTTAPAGYPGGAPSAYPELHAALDRLCAHRSVGFGCQESRHSYPPALGSPGALTGAVVGTAGPLERRGAQPGRHSVTGYGDCAAGARYQDELTALLRLTVATGGREAGARGEPSGIEPSGLEESPGPFVPEASRSRIREPEAREDYFGTCIKCNKGIYGQSNACQALDSLYHTQCFVCCSCGRTLRCKAFYSVNGSVYCEEDYLFSGFQEAAEKCCVCGHLILEKILQAMGKSYHPGCFRCIVCNKCLDGVPFTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVRVISMDRDYHFECYHCEDCRMQLSDEEGCCCFPLDGHLLCHGCHMQRLSARQPSTNYI

Target ORF information:

RefSeq Version	NM_010590.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus ajuba LIM protein (Ajuba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010590.5

ORF Insert Sequence:

ATGGAACGGT TAGGGGAGAA AGCCAGTCGT CTGCTAGAAA AGTTGAGACT CTCGGACTCC 
GGCAGCGCCA AGTTCGGCCG CAGAAAGGGC GAAGCGAGCC GGTCTGGGTC TGATGGGACC 
CCCGGAGCAG GCAAGGGACG CTTAAGCGGG TTGGGGGGAC CTAGGAAGTC AGGACACCGT 
GGAGCGAATG GGGGGCCTGG AGATGAACCT TTGGAACCGG CCAGGGAGCA AGGGCCCCTG 
GACGCCGAGC GGAACGCACG CGGCTCCTTT GAAGCGCAGC GCTTCGAAGG GTCCTTTCCC 
GGGGGGCCGC CGCCCACTCG AGCCCTGCCT CTGCCTCTGT CGTCGCCTCC TGATTTTCGG 
CTGGAGACCA CGGCTCCAGC CCTTAGCCCT CGCTCCAGCT TCGCCAGTAG CTCGGCCAGC 
GATGCGAGCA AGCCATCTAG CCCCCGGGGC AGCCTGCTGC TGGACGGAGC CGGGGCCAGC 
GGAGCCGGAG GTAGCCGGCC GTGCAGCAAT CGGACCAGCG GCATCAGCAT GGGCTACGAC 
CAGCGCCACG GGAGCCCGCT GCCCGCGGGG CCGTGCCTAT TCGGCCTCCC GCTGACCACT 
GCTCCTGCGG GCTACCCTGG AGGGGCTCCG TCCGCCTACC CGGAGCTCCA CGCTGCCCTG 
GACCGACTAT GTGCCCATCG GTCCGTGGGA TTCGGCTGCC AGGAGAGCCG TCACTCGTAC 
CCCCCGGCCC TGGGCAGCCC CGGAGCTCTA ACCGGAGCCG TGGTGGGAAC AGCGGGTCCT 
TTGGAGAGAC GTGGGGCGCA ACCCGGACGA CACTCGGTTA CAGGCTACGG GGACTGCGCC 
GCGGGGGCCC GTTACCAGGA CGAGCTAACA GCATTGCTGC GTTTGACCGT GGCTACCGGT 
GGGCGAGAAG CGGGTGCTCG CGGGGAACCC TCGGGGATTG AGCCGTCGGG TCTGGAGGAG 
TCTCCTGGTC CCTTCGTTCC AGAGGCCTCC CGATCACGGA TACGGGAGCC AGAGGCCAGA 
GAAGATTACT TTGGCACCTG TATCAAGTGC AACAAAGGTA TCTATGGGCA GAGCAATGCC 
TGCCAGGCCC TGGACAGCCT CTACCACACC CAGTGCTTTG TCTGCTGCTC CTGTGGACGA 
ACTTTGCGGT GCAAGGCTTT CTACAGCGTC AATGGCTCTG TCTACTGTGA GGAAGACTAT 
CTGTTTTCAG GGTTTCAGGA GGCAGCTGAG AAGTGCTGTG TCTGCGGCCA CTTGATTCTA 
GAGAAGATCC TCCAGGCCAT GGGGAAGTCC TATCACCCGG GCTGCTTCCG ATGCATCGTA 
TGTAACAAGT GCCTGGACGG CGTCCCCTTC ACTGTGGACT TCTCCAACCA GGTGTACTGT 
GTTACCGACT ACCACAAAAA TTACGCCCCG AAATGTGCAG CCTGCGGACA ACCCATCCTC 
CCCTCAGAGG GCTGTGAGGA CATTGTGAGG GTGATATCCA TGGACCGTGA TTATCACTTT 
GAGTGCTACC ACTGTGAGGA CTGCCGCATG CAGCTGAGTG ACGAGGAAGG TTGCTGCTGT 
TTCCCTCTTG ATGGACATTT GCTCTGCCAC GGCTGTCACA TGCAGCGCCT CAGTGCCCGG 
CAGCCCTCTA CCAACTATAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p63 exerts spatio-temporal control of palatal epithelial cell fate to prevent cleft palate.
PLoS genetics13(6)e1006828(2017 Jun)
Richardson R,Mitchell K,Hammond NL,Mollo MR,Kouwenhoven EN,Wyatt ND,Donaldson IJ,Zeef L,Burgis T,Blance R,van Heeringen SJ,Stunnenberg HG,Zhou H,Missero C,Romano RA,Sinha S,Dixon MJ,Dixon J

The LIM protein Ajuba promotes adipogenesis by enhancing PPAR? and p300/CBP interaction.
Cell death and differentiation23(1)158-68(2016 Jan)
Li Q,Peng H,Fan H,Zou X,Liu Q,Zhang Y,Xu H,Chu Y,Wang C,Ayyanathan K,Rauscher FJ,Zhang K,Hou Z

Ajuba Preferentially Binds LXR?/RXR? Heterodimer to Enhance LXR Target Gene Expression in Liver Cells.
Molecular endocrinology (Baltimore, Md.)29(11)1608-18(2015 Nov)
Fan H,Dong W,Li Q,Zou X,Zhang Y,Wang J,Li S,Liu W,Dong Y,Sun H,Hou Z

A conserved MST1/2-YAP axis mediates Hippo signaling during lung growth.
Developmental biology403(1)101-13(2015 Jul)
Lin C,Yao E,Chuang PT

Adhesion molecule-mediated hippo pathway modulates hemangioendothelioma cell behavior.
Molecular and cellular biology34(24)4485-99(2014 Dec)
Tsuneki M,Madri JA

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Ajuba cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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