Eif6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif6
Entrez Gene ID	16418
Full Name	eukaryotic translation initiation factor 6
Synonyms	1110004P11,AA408895,CAB,Itgb4bp,eIF-6,imc-415,p27(BBP),p27BBP
General protein information	Preferred Names eukaryotic translation initiation factor 6 Names eukaryotic translation initiation factor 6 B4 integrin interactor integrin beta 4 binding protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1
Summary	The protein encoded by this gene is a eukaryotic translation initiation factor that regulates both ribosome biogenesis and translation, which are rate-limiting factors for cell growth. The encoded protein binds 60S ribosomes and prevents their association with 40S ribosomes. This gene may play a role in oncogenesis as well. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010579.2	NP_034709.1	eukaryotic translation initiation factor 6

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF6	NP_001254739.1
Mus musculus (house mouse)	Eif6	NP_034709.1
Saccharomyces cerevisiae (baker's yeast)	TIF6	NP_015341.1
Kluyveromyces lactis	KLLA0D02046g	XP_453159.1
Magnaporthe oryzae (rice blast fungus)	MGG_01671	XP_003714657.1
Pan troglodytes (chimpanzee)	EIF6	XP_003953760.1
Danio rerio (zebrafish)	eif6	NP_957238.1
Eremothecium gossypii	AGOS_ADL030C	NP_984067.1
Arabidopsis thaliana (thale cress)	emb1624	NP_191121.1
Rattus norvegicus (Norway rat)	Eif6	NP_001032429.1
Oryza sativa (rice)	Os07g0639800	NP_001060417.1
Xenopus tropicalis (tropical clawed frog)	eif6	NP_001006884.1
Canis lupus familiaris (dog)	EIF6	XP_534401.2
Bos taurus (cattle)	EIF6	NP_777255.1
Gallus gallus (chicken)	EIF6	NP_001026019.1
Drosophila melanogaster (fruit fly)	eIF6	NP_659573.1
Caenorhabditis elegans (roundworm)	eif-6	NP_493272.1
Neurospora crassa	NCU09004	XP_959032.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001380	XP_321758.5
Schizosaccharomyces pombe (fission yeast)	tif6	NP_588491.1

Gene Ontology
Bibliography

Related articles in PubMed

High levels of eukaryotic Initiation Factor 6 (eIF6) are required for immune system homeostasis and for steering the glycolytic flux of TCR-stimulated CD4
Manfrini N, Ricciardi S, Miluzio A, Fedeli M, Scagliola A, Gallo S, Brina D, Adler T, Busch DH, Gailus-Durner V, Fuchs H, Hrab? de Angelis M, Biffo S
Developmental and comparative immunology7769-76(2017 Dec)

The Role of Eif6 in Skeletal Muscle Homeostasis Revealed by Endurance Training Co-expression Networks.
Clarke K, Ricciardi S, Pearson T, Bharudin I, Davidsen PK, Bonomo M, Brina D, Scagliola A, Simpson DM, Beynon RJ, Khanim F, Ankers J, Sarzynski MA, Ghosh S, Pisconti A, Rozman J, Hrabe de Angelis M, Bunce C, Stewart C, Egginton S, Caddick M, Jackson M, Bouchard C, Biffo S, Falciani F
Cell reports21(6)1507-1520(2017 Nov)

Involvement of eIF6 in external mechanical stretch-mediated murine dermal fibroblast function via TGF-?1 pathway.
Shu Q, Tan J, Ulrike VD, Zhang X, Yang J, Yang S, Hu X, He W, Luo G, Wu J
Scientific reports636075(2016 Nov)

Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair.
Bai YQ, Feng JK, Zhang QF, Wu HZ, Du YR, Wei W
Molecular medicine reports14(1)819-24(2016 Jul)

Eukaryotic initiation factor 6 modulates myofibroblast differentiation at transforming growth factor-?1 transcription level via H2A.Z occupancy and Sp1 recruitment.
Yang SS, Tan JL, Liu DS, Loreni F, Peng X, Yang QQ, He WF, Yao ZH, Zhang XR, Dal Pr? I, Luo GX, Wu J
Journal of cell science128(21)3977-89(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

eIF6 relieved fibrosis/cicatrization by inhibiting the generation of VEGF to prevent the overgrowth of blood vessels and production of granulation tissues, and by regulating the MMP-2/TIMP-2 balance to promote ECM degradation and decrease its deposition.
Title: Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair.

Reduced eIF6 expression negatively impacts on megakaryopoiesis. in eIF6(+/-) cells, the steady-state abundance of mitochondrial respiratory chain complex I-encoding mRNAs is decreased, resulting in decreased reactive oxygen species production.
Title: Eukaryotic translation initiation factor 6 is a novel regulator of reactive oxygen species-dependent megakaryocyte maturation.

data reveal a novel transcriptional regulatory mechanism of eIF6 that acts on facilitating Sp1 recruitment to TGF-beta1 promoter via H2A.Z depletion and thus results in increased TGF-beta1 transcription, which contributes to myofibroblast differentiation.
Title: Eukaryotic initiation factor 6 modulates myofibroblast differentiation at transforming growth factor-?1 transcription level via H2A.Z occupancy and Sp1 recruitment.

eIF6 controls fatty acid synthesis and glycolysis in a cell autonomous fashion. Translational activation by eIF6 reshapes gene expression with increased levels of lipogenic and glycolytic enzymes.
Title: eIF6 coordinates insulin sensitivity and lipid metabolism by coupling translation to transcription.

In a murine model of lymphomagenesis, eIF6 depletion leads to a striking increase of survival, without adverse effects.
Title: eIF6 anti-association activity is required for ribosome biogenesis, translational control and tumor progression.

The following Eif6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06381	NM_010579.2 Latest version!	Mus musculus eukaryotic translation initiation factor 6 (Eif6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif6 ( NM_010579.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034709.1 Mus musculus eukaryotic translation initiation factor 6 (Eif6), mRNA.

Price & Availability↑

CloneID	OMu06381
Clone ID Related Accession (Same CDS sequence)	NM_010579.2
Accession Version	NM_010579.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	eukaryotic translation initiation factor 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL845445.14, BX004872.6 and AL929233.9. On Mar 21, 2007 this sequence version replaced NM_010579.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015274.1, AF108462.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCA GAGCGTCGTT CGAGAACAAC TGTGAGGTCG GTTGTTTTGC CAAACTCACA 
AACGCCTACT GCCTGGTGGC CATCGGAGGC TCAGAGAACT TCTATAGTGT GTTCGAGGGT 
GAGCTCTCCG ATGCCATTCC CGTGGTGCAC GCATCCATCG CCGGCTGCCG AATCATCGGG 
CGCATGTGTG TGGGGAACAG GCATGGGCTC CTGGTACCCA ACAACACCAC CGACCAGGAG 
CTGCAGCACA TCCGCAACAG CCTGCCTGAC TCCGTGCAGA TACGGCGGGT GGAGGAGCGG 
CTCTCGGCCC TTGGCAATGT CACCACCTGC AATGACTATG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA GATCCTGGCT GATGTCCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTTGCTG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGTAATCA GGGGGGGCTG 
GTGCACCCTA AAACTTCTAT CGAGGACCAG GATGAGTTGT CCTCCCTTCT TCAGGTCCCC 
CTTGTGGCAG GCACTGTGAA CCGAGGGAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAC 
GATTGGTGTG CTTTCTGTGG TCTGGACACG ACCAGCACGG AGCTGTCAGT GGTGGAGAGC 
GTCTTCAAGC TGAATGAAGC CAAGCCAAGT ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034709.1
CDS	493..1230
Translation	MAVRASFENNCEVGCFAKLTNAYCLVAIGGSENFYSVFEGELSDAIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDSVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAKPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_010579.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation initiation factor 6 (Eif6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010579.2

ORF Insert Sequence:

ATGGCGGTCA GAGCGTCGTT CGAGAACAAC TGTGAGGTCG GTTGTTTTGC CAAACTCACA 
AACGCCTACT GCCTGGTGGC CATCGGAGGC TCAGAGAACT TCTATAGTGT GTTCGAGGGT 
GAGCTCTCCG ATGCCATTCC CGTGGTGCAC GCATCCATCG CCGGCTGCCG AATCATCGGG 
CGCATGTGTG TGGGGAACAG GCATGGGCTC CTGGTACCCA ACAACACCAC CGACCAGGAG 
CTGCAGCACA TCCGCAACAG CCTGCCTGAC TCCGTGCAGA TACGGCGGGT GGAGGAGCGG 
CTCTCGGCCC TTGGCAATGT CACCACCTGC AATGACTATG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA GATCCTGGCT GATGTCCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTTGCTG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGTAATCA GGGGGGGCTG 
GTGCACCCTA AAACTTCTAT CGAGGACCAG GATGAGTTGT CCTCCCTTCT TCAGGTCCCC 
CTTGTGGCAG GCACTGTGAA CCGAGGGAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAC 
GATTGGTGTG CTTTCTGTGG TCTGGACACG ACCAGCACGG AGCTGTCAGT GGTGGAGAGC 
GTCTTCAAGC TGAATGAAGC CAAGCCAAGT ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High levels of eukaryotic Initiation Factor 6 (eIF6) are required for immune system homeostasis and for steering the glycolytic flux of TCR-stimulated CD4
Developmental and comparative immunology7769-76(2017 Dec)
Manfrini N,Ricciardi S,Miluzio A,Fedeli M,Scagliola A,Gallo S,Brina D,Adler T,Busch DH,Gailus-Durner V,Fuchs H,Hrab? de Angelis M,Biffo S

The Role of Eif6 in Skeletal Muscle Homeostasis Revealed by Endurance Training Co-expression Networks.
Cell reports21(6)1507-1520(2017 Nov)
Clarke K,Ricciardi S,Pearson T,Bharudin I,Davidsen PK,Bonomo M,Brina D,Scagliola A,Simpson DM,Beynon RJ,Khanim F,Ankers J,Sarzynski MA,Ghosh S,Pisconti A,Rozman J,Hrabe de Angelis M,Bunce C,Stewart C,Egginton S,Caddick M,Jackson M,Bouchard C,Biffo S,Falciani F

Involvement of eIF6 in external mechanical stretch-mediated murine dermal fibroblast function via TGF-?1 pathway.
Scientific reports636075(2016 Nov)
Shu Q,Tan J,Ulrike VD,Zhang X,Yang J,Yang S,Hu X,He W,Luo G,Wu J

Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair.
Molecular medicine reports14(1)819-24(2016 Jul)
Bai YQ,Feng JK,Zhang QF,Wu HZ,Du YR,Wei W

Eukaryotic initiation factor 6 modulates myofibroblast differentiation at transforming growth factor-?1 transcription level via H2A.Z occupancy and Sp1 recruitment.
Journal of cell science128(21)3977-89(2015 Nov)
Yang SS,Tan JL,Liu DS,Loreni F,Peng X,Yang QQ,He WF,Yao ZH,Zhang XR,Dal Pr? I,Luo GX,Wu J

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Eif6 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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