Itgax cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itgax
Entrez Gene ID	16411
Full Name	integrin alpha X
Synonyms	AI449405,Cd11c,Cr4,N418
General protein information	Preferred Names integrin alpha X Names integrin alpha-X CD11 antigen-like family member C CD11C (p150) alpha polypeptide complement receptor 4 integrin aX leukocyte adhesion glycoprotein p150,95 alpha chain leukocyte adhesion receptor p150,95
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001363985.1	NP_001350914.1	integrin alpha-X isoform 3 precursor
NM_001363984.1	NP_001350913.1	integrin alpha-X isoform 2 precursor
NM_021334.3	NP_067309.1	integrin alpha-X isoform 1 precursor
NM_021334.2	NP_067309.1	integrin alpha-X precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ITGAX	XP_510951.3
Homo sapiens (human)	ITGAX	NP_001273304.1
Canis lupus familiaris (dog)	ITGAX	XP_547049.4
Rattus norvegicus (Norway rat)	Itgax	XP_574569.3
Mus musculus (house mouse)	Itgax	NP_067309.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel microglial subset plays a key role in myelinogenesis in developing brain.
Wlodarczyk A, Holtman IR, Krueger M, Yogev N, Bruttger J, Khorooshi R, Benmamar-Badel A, de Boer-Bergsma JJ, Martin NA, Karram K, Kramer I, Boddeke EW, Waisman A, Eggen BJ, Owens T
The EMBO journal36(22)3292-3308(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that the autoimmune phenotype in DEF6 and SWAP-70 knock-out (DKO) mice is characterized by the accumulation of B cell subset that expresses high levels of CD11c and T-bet (ABCs).
Title: Regulation of systemic autoimmunity and CD11c

Results implicate a monocytic origin of CD11c(+) cells in inflamed islets and suggest that therapeutic regulatory T cells directly or indirectly regulate their influx by altering the chemotactic milieu in the islets.
Title: Cutting Edge: Origins, Recruitment, and Regulation of CD11c

this study shows that PU.1 functions as a positive regulator of CD11c gene expression by directly binding to the Itgax promoter and through transactivation of the Irf4 gene
Title: The hematopoietic cell-specific transcription factor PU.1 is critical for expression of CD11c.

Data concluded that the ablation of CD11c(hi) dendritic cells provided a subsidiary impact on blood-brain barrier (BBB) integrity and the expression of tight junction/adhesion molecules, thereby leading to exacerbated Japanese encephalitis (JE) progression.
Title: Ablation of CD11c(hi) dendritic cells exacerbates Japanese encephalitis by regulating blood-brain barrier permeability and altering tight junction/adhesion molecules.

the CD11c promoter was unexpectedly active and triggered Mgat2 deletion within multiple hematopoietic lineages, showed remarkably poor penetrance within native DC populations, and produced almost undetectable levels of green fluorescent protein signal. These findings show that the CD11c promoter is not DC-specific, and extreme care should be taken in the interpretation of data using any mouse created using the CD11c-CRE
Title: Dendritic cell-specific Mgat2 knockout mice show antigen presentation defects but reveal an unexpected CD11c expression pattern.

The following Itgax gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itgax cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84310	NM_001363984.1 Latest version!	Mus musculus integrin alpha X (Itgax), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu84385	NM_001363985.1 Latest version!	Mus musculus integrin alpha X (Itgax), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu16044	NM_021334.3 Latest version!	Mus musculus integrin alpha X (Itgax), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu16044	NM_021334.2	Mus musculus integrin alpha X (Itgax), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itgax ( NM_001363984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350913.1 Mus musculus integrin alpha X (Itgax), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84310
Clone ID Related Accession (Same CDS sequence)	NM_001363984.1
Accession Version	NM_001363984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	integrin alpha-X isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124566.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057200.1, BI645825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACAGAACTG 
TTCACCACCC AAAGTGGAGC TCGGCAAGAT GCCACCAAGG TCCTCATTGT CATCACTGAT 
GGGAGAAAAC AAGGGGACAA CTTGAGTTAT GATAGTGTCA TCCCCATGGC AGAGGCTGCA 
AGCATCATTC GTTATGCAAT TGGGGTAGGA CACAAAGATG GTTTCCCACC ACTTCCTCCT 
GTAACTTCCT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350913.1
CDS	71..985
Translation	MSCTWIAFLLLLGFVSCLGFNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKATNQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENIYLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMSQLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITELFTTQSGARQDATKVLIVITDGRKQGDNLSYDSVIPMAEAASIIRYAIGVGHKDGFPPLPPVTSS

Target ORF information:

RefSeq Version	NM_001363984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha X (Itgax), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363984.1

ORF Insert Sequence:

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACAGAACTG 
TTCACCACCC AAAGTGGAGC TCGGCAAGAT GCCACCAAGG TCCTCATTGT CATCACTGAT 
GGGAGAAAAC AAGGGGACAA CTTGAGTTAT GATAGTGTCA TCCCCATGGC AGAGGCTGCA 
AGCATCATTC GTTATGCAAT TGGGGTAGGA CACAAAGATG GTTTCCCACC ACTTCCTCCT 
GTAACTTCCT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgax ( NM_001363985.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350914.1 Mus musculus integrin alpha X (Itgax), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84385
Clone ID Related Accession (Same CDS sequence)	NM_001363985.1
Accession Version	NM_001363985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	integrin alpha-X isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124566.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK089167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACTGTTCAC 
CACCCAAAGT GGAGCTCGGC AAGATGCCAC CAAGGTCCTC ATTGTCATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350914.1
CDS	71..844
Translation	MSCTWIAFLLLLGFVSCLGFNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKATNQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENIYLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMSQLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITVHHPKWSSARCHQGPHCHH

Target ORF information:

RefSeq Version	NM_001363985.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha X (Itgax), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363985.1

ORF Insert Sequence:

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACTGTTCAC 
CACCCAAAGT GGAGCTCGGC AAGATGCCAC CAAGGTCCTC ATTGTCATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgax ( NM_021334.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067309.1 Mus musculus integrin alpha X (Itgax), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16044
Clone ID Related Accession (Same CDS sequence)	NM_021334.3 , NM_021334.2
Accession Version	NM_021334.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	integrin alpha-X isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124566.5. On Jun 3, 2018 this sequence version replaced NM_021334.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF211864.1, AK155816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACAGAACTG 
TTCACCACCC AAAGTGGAGC TCGGCAAGAT GCCACCAAGG TCCTCATTGT CATCACTGAT 
GGGAGAAAAC AAGGGGACAA CTTGAGTTAT GATAGTGTCA TCCCCATGGC AGAGGCTGCA 
AGCATCATTC GTTATGCAAT TGGGGTAGGA AAGGCCTTTT ACAATGAACA TTCCAAGCAA 
GAATTAAAGG CCATTGCATC GATGCCTTCC CATGAATACG TATTCAGCGT GGAGAACTTT 
GATGCTTTGA AGGATATTGA GAATCAGCTG AAGGAGAAGA TCTTTGCCAT TGAGGGCACA 
GAGACACCAA GCAGCAGTAC TTTTGAATTG GAGATGTCCC AGGAGGGCTT CAGTGCTGTG 
TTTACACCTG ATGGACCAGT TCTGGGGGCT GTGGGAAGCT TCAGCTGGTC TGGAGGTGCC 
TTCTTGTACC CCTCAAATAT GAGACCCACC TTCATCAACA TGTCTCAGGA GAACGAGGAC 
ATGAGGGACG CTTACCTGGG TTACTCCACC GCACTGGCCT TTTGGAAGGG GGTCCACAGC 
CTGATCCTGG GGGCCCCTCG CCACCAGCAC ACGGGGAAGG TTGTCATCTT TACCCAGGAA 
TCCAGGCACT GGAGGCCCAA GTCTGAAGTC AGAGGGACAC AGATCGGCTC CTACTTTGGG 
GCATCTCTTT GTTCTGTGGA CATGGATAGA GATGGCAGCA CTGACCTGGT CCTGATTGGA 
GTCCCCCATT ACTATGAGCA CACCCGAGGG GGGCAGGTGT CAGTGTGCCC CATGCCCGGT 
GTGGGGAGCA GGTGGCATTG TGGGACCACC CTCCATGGGG AGCAGGGCCA TCCTTGGGGC 
CGCTTTGGGG CGGCTCTGAC AGTGCTAGGG GACGTGAATG GGGACAGTCT GGCAGATGTG 
GCTATTGGTG CACCCGGAGA GGAGGAGAAC AGAGGTGCTG TCTACATATT TCATGGAGCC 
TCAAGACAGG ACATCGCTCC CTCACCCAGC CAGAGGATTT CAGCATCCCA GATCCCCTCC 
AGGATTCAAT ATTTTGGGCA GTCACTGAGT GGGGGCCAGG ACCTCACCCG GGATGGACTG 
GTGGATCTGG CCGTGGGCTC CAAGGGACGC GTGCTGCTGC TCAGGACCAG ACCAATTCTT 
AGAGTGTCAC CAACTGTTCA CTTCACACCT GCAGAGATTT CCAGGTCTGT GTTTGAGTGT 
CAGGAGCAGG TGGCCCCTGA GCAGACACTG AGTGATGCCA CTGTCTGCCT TCATATTCAT 
GAAAGCCCCA AGACCCAACT AGGTGACCTC CGAAGTACTG TCACCTTTGA CCTGGCTCTA 
GACCATGGCC GTCTGAGTAC CCGGGCCATC TTCAAGGAGA CAAAGACCCG GGCTCTGACT 
CGAGTTAAAA CTCTTGGTCT GAACAAGCAC TGTGAAAGTG TGAAGTTGCT TCTCCCGGCC 
TGTGTGGAGG ACTCAGTGAC CCCGATCACT CTTCGCCTCA ACTTTTCTCT TGTTGGTGTG 
CCCATCAGTT CCTTACAAAA TCTCCAACCC ATGCTGGCTG TAGATGACCA AACGTACTTC 
ACGGCCTCTC TTCCCTTTGA GAAGAACTGT GGAGCTGACC ACATCTGCCA GGATGACCTT 
AGTGTCGTAT TTGGCTTCCC AGACTTGAAG ACCCTGGTGG TGGGAAGTGA CCTGGAGCTG 
AATGTAGATG TGACGGTGTC TAATGATGGA GAAGATTCCT ACGGAACCAC AGTCACTCTG 
TTCTACCCCG TGGGTCTGTC CTTTAGACGA GTTGCAGAAG GCCAAGTATT CCTTCGGAAG 
AAAGAGGACC AGCAGTGGCA GCGGCGTGGG CAGCACTCCC TGCACCTCAT GTGTGACAGC 
ACCCCAGACA GGAGTCAGGG CCTCTGGAGT ACCAGCTGTA GCAGCAGACA TGTCATTTTC 
CGTGGAGGCT CACAGATGAC TTTTTTGGTA ACTTTTGACG TCTCCCCCAA AGCTGAGCTG 
GGGGACCGGC TGCTTCTAAG AGCCAGAGTG GGAAGTGAGA ATAATGTACC CGGAACCCCA 
AAAACCACCT TCCAGTTGGA GCTTCCAGTA AAATATGCGG TGTACACCAT GATCAGCAGC 
CATGACCAGT TTACCAAGTA TCTTAACTTC TCAACCTCAG AAAAGGAGAA GACCAGTGTG 
GTCGAACATA GATTCCAGGT GAATAACCTG GGCCAGAGAG ACGTGCCAGT CAGCATCAAC 
TTTTGGGTGC CCATAGAGCT GAAGGGAGAA GCTGTGTGGA CAGTGATGGT CTCCCACCCT 
CAGAACCCAC TCACCCAATG CTATCGGAAT AGACTCAAGC CAACACAATT TGACCTCCTG 
ACTCACATGC AAAAGAGTCC CGTGCTGGAC TGCTCCATCG CTGACTGCCT GCACCTCCGC 
TGTGACATCC CCTCCTTGGG CATCCTGGAT GAGCTTTACT TCATTCTGAA GGGCAACCTC 
AGCTTCGGCT GGATCAGTCA GACATTGCAG AAAAAGGTGT TGCTCCTGAG TGAGGCTGAA 
ATCACATTCA ACACATCTGT GTATTCCCAG CTGCCGGGAC AGGAGGCATT TCTGAGAGCC 
CAGACGAAGA CAGTGCTGGA GATGTATAAA GTTCACAACC CCGTCCCTCT TATCGTGGGC 
AGCTCAGTGG GAGGTCTGCT GCTGCTGGCT ATCATCACAG CTATACTTTA CAAGGCTGGT 
TTCTTCAAAC GTCAGTACAA GGAGATGTTG GAGGAAGCAA ATGGACAGTT TGTCTCAGAC 
GGGACCCCAA CTCCCCAGGT TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067309.1
CDS	71..3580
Translation	MSCTWIAFLLLLGFVSCLGFNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKATNQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENIYLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMSQLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITELFTTQSGARQDATKVLIVITDGRKQGDNLSYDSVIPMAEAASIIRYAIGVGKAFYNEHSKQELKAIASMPSHEYVFSVENFDALKDIENQLKEKIFAIEGTETPSSSTFELEMSQEGFSAVFTPDGPVLGAVGSFSWSGGAFLYPSNMRPTFINMSQENEDMRDAYLGYSTALAFWKGVHSLILGAPRHQHTGKVVIFTQESRHWRPKSEVRGTQIGSYFGASLCSVDMDRDGSTDLVLIGVPHYYEHTRGGQVSVCPMPGVGSRWHCGTTLHGEQGHPWGRFGAALTVLGDVNGDSLADVAIGAPGEEENRGAVYIFHGASRQDIAPSPSQRISASQIPSRIQYFGQSLSGGQDLTRDGLVDLAVGSKGRVLLLRTRPILRVSPTVHFTPAEISRSVFECQEQVAPEQTLSDATVCLHIHESPKTQLGDLRSTVTFDLALDHGRLSTRAIFKETKTRALTRVKTLGLNKHCESVKLLLPACVEDSVTPITLRLNFSLVGVPISSLQNLQPMLAVDDQTYFTASLPFEKNCGADHICQDDLSVVFGFPDLKTLVVGSDLELNVDVTVSNDGEDSYGTTVTLFYPVGLSFRRVAEGQVFLRKKEDQQWQRRGQHSLHLMCDSTPDRSQGLWSTSCSSRHVIFRGGSQMTFLVTFDVSPKAELGDRLLLRARVGSENNVPGTPKTTFQLELPVKYAVYTMISSHDQFTKYLNFSTSEKEKTSVVEHRFQVNNLGQRDVPVSINFWVPIELKGEAVWTVMVSHPQNPLTQCYRNRLKPTQFDLLTHMQKSPVLDCSIADCLHLRCDIPSLGILDELYFILKGNLSFGWISQTLQKKVLLLSEAEITFNTSVYSQLPGQEAFLRAQTKTVLEMYKVHNPVPLIVGSSVGGLLLLAIITAILYKAGFFKRQYKEMLEEANGQFVSDGTPTPQVAQ

Target ORF information:

RefSeq Version	NM_021334.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha X (Itgax), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021334.3

ORF Insert Sequence:

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACAGAACTG 
TTCACCACCC AAAGTGGAGC TCGGCAAGAT GCCACCAAGG TCCTCATTGT CATCACTGAT 
GGGAGAAAAC AAGGGGACAA CTTGAGTTAT GATAGTGTCA TCCCCATGGC AGAGGCTGCA 
AGCATCATTC GTTATGCAAT TGGGGTAGGA AAGGCCTTTT ACAATGAACA TTCCAAGCAA 
GAATTAAAGG CCATTGCATC GATGCCTTCC CATGAATACG TATTCAGCGT GGAGAACTTT 
GATGCTTTGA AGGATATTGA GAATCAGCTG AAGGAGAAGA TCTTTGCCAT TGAGGGCACA 
GAGACACCAA GCAGCAGTAC TTTTGAATTG GAGATGTCCC AGGAGGGCTT CAGTGCTGTG 
TTTACACCTG ATGGACCAGT TCTGGGGGCT GTGGGAAGCT TCAGCTGGTC TGGAGGTGCC 
TTCTTGTACC CCTCAAATAT GAGACCCACC TTCATCAACA TGTCTCAGGA GAACGAGGAC 
ATGAGGGACG CTTACCTGGG TTACTCCACC GCACTGGCCT TTTGGAAGGG GGTCCACAGC 
CTGATCCTGG GGGCCCCTCG CCACCAGCAC ACGGGGAAGG TTGTCATCTT TACCCAGGAA 
TCCAGGCACT GGAGGCCCAA GTCTGAAGTC AGAGGGACAC AGATCGGCTC CTACTTTGGG 
GCATCTCTTT GTTCTGTGGA CATGGATAGA GATGGCAGCA CTGACCTGGT CCTGATTGGA 
GTCCCCCATT ACTATGAGCA CACCCGAGGG GGGCAGGTGT CAGTGTGCCC CATGCCCGGT 
GTGGGGAGCA GGTGGCATTG TGGGACCACC CTCCATGGGG AGCAGGGCCA TCCTTGGGGC 
CGCTTTGGGG CGGCTCTGAC AGTGCTAGGG GACGTGAATG GGGACAGTCT GGCAGATGTG 
GCTATTGGTG CACCCGGAGA GGAGGAGAAC AGAGGTGCTG TCTACATATT TCATGGAGCC 
TCAAGACAGG ACATCGCTCC CTCACCCAGC CAGAGGATTT CAGCATCCCA GATCCCCTCC 
AGGATTCAAT ATTTTGGGCA GTCACTGAGT GGGGGCCAGG ACCTCACCCG GGATGGACTG 
GTGGATCTGG CCGTGGGCTC CAAGGGACGC GTGCTGCTGC TCAGGACCAG ACCAATTCTT 
AGAGTGTCAC CAACTGTTCA CTTCACACCT GCAGAGATTT CCAGGTCTGT GTTTGAGTGT 
CAGGAGCAGG TGGCCCCTGA GCAGACACTG AGTGATGCCA CTGTCTGCCT TCATATTCAT 
GAAAGCCCCA AGACCCAACT AGGTGACCTC CGAAGTACTG TCACCTTTGA CCTGGCTCTA 
GACCATGGCC GTCTGAGTAC CCGGGCCATC TTCAAGGAGA CAAAGACCCG GGCTCTGACT 
CGAGTTAAAA CTCTTGGTCT GAACAAGCAC TGTGAAAGTG TGAAGTTGCT TCTCCCGGCC 
TGTGTGGAGG ACTCAGTGAC CCCGATCACT CTTCGCCTCA ACTTTTCTCT TGTTGGTGTG 
CCCATCAGTT CCTTACAAAA TCTCCAACCC ATGCTGGCTG TAGATGACCA AACGTACTTC 
ACGGCCTCTC TTCCCTTTGA GAAGAACTGT GGAGCTGACC ACATCTGCCA GGATGACCTT 
AGTGTCGTAT TTGGCTTCCC AGACTTGAAG ACCCTGGTGG TGGGAAGTGA CCTGGAGCTG 
AATGTAGATG TGACGGTGTC TAATGATGGA GAAGATTCCT ACGGAACCAC AGTCACTCTG 
TTCTACCCCG TGGGTCTGTC CTTTAGACGA GTTGCAGAAG GCCAAGTATT CCTTCGGAAG 
AAAGAGGACC AGCAGTGGCA GCGGCGTGGG CAGCACTCCC TGCACCTCAT GTGTGACAGC 
ACCCCAGACA GGAGTCAGGG CCTCTGGAGT ACCAGCTGTA GCAGCAGACA TGTCATTTTC 
CGTGGAGGCT CACAGATGAC TTTTTTGGTA ACTTTTGACG TCTCCCCCAA AGCTGAGCTG 
GGGGACCGGC TGCTTCTAAG AGCCAGAGTG GGAAGTGAGA ATAATGTACC CGGAACCCCA 
AAAACCACCT TCCAGTTGGA GCTTCCAGTA AAATATGCGG TGTACACCAT GATCAGCAGC 
CATGACCAGT TTACCAAGTA TCTTAACTTC TCAACCTCAG AAAAGGAGAA GACCAGTGTG 
GTCGAACATA GATTCCAGGT GAATAACCTG GGCCAGAGAG ACGTGCCAGT CAGCATCAAC 
TTTTGGGTGC CCATAGAGCT GAAGGGAGAA GCTGTGTGGA CAGTGATGGT CTCCCACCCT 
CAGAACCCAC TCACCCAATG CTATCGGAAT AGACTCAAGC CAACACAATT TGACCTCCTG 
ACTCACATGC AAAAGAGTCC CGTGCTGGAC TGCTCCATCG CTGACTGCCT GCACCTCCGC 
TGTGACATCC CCTCCTTGGG CATCCTGGAT GAGCTTTACT TCATTCTGAA GGGCAACCTC 
AGCTTCGGCT GGATCAGTCA GACATTGCAG AAAAAGGTGT TGCTCCTGAG TGAGGCTGAA 
ATCACATTCA ACACATCTGT GTATTCCCAG CTGCCGGGAC AGGAGGCATT TCTGAGAGCC 
CAGACGAAGA CAGTGCTGGA GATGTATAAA GTTCACAACC CCGTCCCTCT TATCGTGGGC 
AGCTCAGTGG GAGGTCTGCT GCTGCTGGCT ATCATCACAG CTATACTTTA CAAGGCTGGT 
TTCTTCAAAC GTCAGTACAA GGAGATGTTG GAGGAAGCAA ATGGACAGTT TGTCTCAGAC 
GGGACCCCAA CTCCCCAGGT TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itgax ( NM_021334.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067309.1 Mus musculus integrin alpha X (Itgax), mRNA.

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CloneID	OMu16044
Clone ID Related Accession (Same CDS sequence)	NM_021334.3 , NM_021334.2
Accession Version	NM_021334.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Mus musculus(house mouse)
Product	integrin alpha-X precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC124566.5. On Nov 17, 2006 this sequence version replaced NM_021334.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF211864.1, AK131133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACAGAACTG 
TTCACCACCC AAAGTGGAGC TCGGCAAGAT GCCACCAAGG TCCTCATTGT CATCACTGAT 
GGGAGAAAAC AAGGGGACAA CTTGAGTTAT GATAGTGTCA TCCCCATGGC AGAGGCTGCA 
AGCATCATTC GTTATGCAAT TGGGGTAGGA AAGGCCTTTT ACAATGAACA TTCCAAGCAA 
GAATTAAAGG CCATTGCATC GATGCCTTCC CATGAATACG TATTCAGCGT GGAGAACTTT 
GATGCTTTGA AGGATATTGA GAATCAGCTG AAGGAGAAGA TCTTTGCCAT TGAGGGCACA 
GAGACACCAA GCAGCAGTAC TTTTGAATTG GAGATGTCCC AGGAGGGCTT CAGTGCTGTG 
TTTACACCTG ATGGACCAGT TCTGGGGGCT GTGGGAAGCT TCAGCTGGTC TGGAGGTGCC 
TTCTTGTACC CCTCAAATAT GAGACCCACC TTCATCAACA TGTCTCAGGA GAACGAGGAC 
ATGAGGGACG CTTACCTGGG TTACTCCACC GCACTGGCCT TTTGGAAGGG GGTCCACAGC 
CTGATCCTGG GGGCCCCTCG CCACCAGCAC ACGGGGAAGG TTGTCATCTT TACCCAGGAA 
TCCAGGCACT GGAGGCCCAA GTCTGAAGTC AGAGGGACAC AGATCGGCTC CTACTTTGGG 
GCATCTCTTT GTTCTGTGGA CATGGATAGA GATGGCAGCA CTGACCTGGT CCTGATTGGA 
GTCCCCCATT ACTATGAGCA CACCCGAGGG GGGCAGGTGT CAGTGTGCCC CATGCCCGGT 
GTGGGGAGCA GGTGGCATTG TGGGACCACC CTCCATGGGG AGCAGGGCCA TCCTTGGGGC 
CGCTTTGGGG CGGCTCTGAC AGTGCTAGGG GACGTGAATG GGGACAGTCT GGCAGATGTG 
GCTATTGGTG CACCCGGAGA GGAGGAGAAC AGAGGTGCTG TCTACATATT TCATGGAGCC 
TCAAGACAGG ACATCGCTCC CTCACCCAGC CAGAGGATTT CAGCATCCCA GATCCCCTCC 
AGGATTCAAT ATTTTGGGCA GTCACTGAGT GGGGGCCAGG ACCTCACCCG GGATGGACTG 
GTGGATCTGG CCGTGGGCTC CAAGGGACGC GTGCTGCTGC TCAGGACCAG ACCAATTCTT 
AGAGTGTCAC CAACTGTTCA CTTCACACCT GCAGAGATTT CCAGGTCTGT GTTTGAGTGT 
CAGGAGCAGG TGGCCCCTGA GCAGACACTG AGTGATGCCA CTGTCTGCCT TCATATTCAT 
GAAAGCCCCA AGACCCAACT AGGTGACCTC CGAAGTACTG TCACCTTTGA CCTGGCTCTA 
GACCATGGCC GTCTGAGTAC CCGGGCCATC TTCAAGGAGA CAAAGACCCG GGCTCTGACT 
CGAGTTAAAA CTCTTGGTCT GAACAAGCAC TGTGAAAGTG TGAAGTTGCT TCTCCCGGCC 
TGTGTGGAGG ACTCAGTGAC CCCGATCACT CTTCGCCTCA ACTTTTCTCT TGTTGGTGTG 
CCCATCAGTT CCTTACAAAA TCTCCAACCC ATGCTGGCTG TAGATGACCA AACGTACTTC 
ACGGCCTCTC TTCCCTTTGA GAAGAACTGT GGAGCTGACC ACATCTGCCA GGATGACCTT 
AGTGTCGTAT TTGGCTTCCC AGACTTGAAG ACCCTGGTGG TGGGAAGTGA CCTGGAGCTG 
AATGTAGATG TGACGGTGTC TAATGATGGA GAAGATTCCT ACGGAACCAC AGTCACTCTG 
TTCTACCCCG TGGGTCTGTC CTTTAGACGA GTTGCAGAAG GCCAAGTATT CCTTCGGAAG 
AAAGAGGACC AGCAGTGGCA GCGGCGTGGG CAGCACTCCC TGCACCTCAT GTGTGACAGC 
ACCCCAGACA GGAGTCAGGG CCTCTGGAGT ACCAGCTGTA GCAGCAGACA TGTCATTTTC 
CGTGGAGGCT CACAGATGAC TTTTTTGGTA ACTTTTGACG TCTCCCCCAA AGCTGAGCTG 
GGGGACCGGC TGCTTCTAAG AGCCAGAGTG GGAAGTGAGA ATAATGTACC CGGAACCCCA 
AAAACCACCT TCCAGTTGGA GCTTCCAGTA AAATATGCGG TGTACACCAT GATCAGCAGC 
CATGACCAGT TTACCAAGTA TCTTAACTTC TCAACCTCAG AAAAGGAGAA GACCAGTGTG 
GTCGAACATA GATTCCAGGT GAATAACCTG GGCCAGAGAG ACGTGCCAGT CAGCATCAAC 
TTTTGGGTGC CCATAGAGCT GAAGGGAGAA GCTGTGTGGA CAGTGATGGT CTCCCACCCT 
CAGAACCCAC TCACCCAATG CTATCGGAAT AGACTCAAGC CAACACAATT TGACCTCCTG 
ACTCACATGC AAAAGAGTCC CGTGCTGGAC TGCTCCATCG CTGACTGCCT GCACCTCCGC 
TGTGACATCC CCTCCTTGGG CATCCTGGAT GAGCTTTACT TCATTCTGAA GGGCAACCTC 
AGCTTCGGCT GGATCAGTCA GACATTGCAG AAAAAGGTGT TGCTCCTGAG TGAGGCTGAA 
ATCACATTCA ACACATCTGT GTATTCCCAG CTGCCGGGAC AGGAGGCATT TCTGAGAGCC 
CAGACGAAGA CAGTGCTGGA GATGTATAAA GTTCACAACC CCGTCCCTCT TATCGTGGGC 
AGCTCAGTGG GAGGTCTGCT GCTGCTGGCT ATCATCACAG CTATACTTTA CAAGGCTGGT 
TTCTTCAAAC GTCAGTACAA GGAGATGTTG GAGGAAGCAA ATGGACAGTT TGTCTCAGAC 
GGGACCCCAA CTCCCCAGGT TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067309.1
CDS	53..3562
Translation	MSCTWIAFLLLLGFVSCLGFNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKATNQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENIYLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMSQLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITELFTTQSGARQDATKVLIVITDGRKQGDNLSYDSVIPMAEAASIIRYAIGVGKAFYNEHSKQELKAIASMPSHEYVFSVENFDALKDIENQLKEKIFAIEGTETPSSSTFELEMSQEGFSAVFTPDGPVLGAVGSFSWSGGAFLYPSNMRPTFINMSQENEDMRDAYLGYSTALAFWKGVHSLILGAPRHQHTGKVVIFTQESRHWRPKSEVRGTQIGSYFGASLCSVDMDRDGSTDLVLIGVPHYYEHTRGGQVSVCPMPGVGSRWHCGTTLHGEQGHPWGRFGAALTVLGDVNGDSLADVAIGAPGEEENRGAVYIFHGASRQDIAPSPSQRISASQIPSRIQYFGQSLSGGQDLTRDGLVDLAVGSKGRVLLLRTRPILRVSPTVHFTPAEISRSVFECQEQVAPEQTLSDATVCLHIHESPKTQLGDLRSTVTFDLALDHGRLSTRAIFKETKTRALTRVKTLGLNKHCESVKLLLPACVEDSVTPITLRLNFSLVGVPISSLQNLQPMLAVDDQTYFTASLPFEKNCGADHICQDDLSVVFGFPDLKTLVVGSDLELNVDVTVSNDGEDSYGTTVTLFYPVGLSFRRVAEGQVFLRKKEDQQWQRRGQHSLHLMCDSTPDRSQGLWSTSCSSRHVIFRGGSQMTFLVTFDVSPKAELGDRLLLRARVGSENNVPGTPKTTFQLELPVKYAVYTMISSHDQFTKYLNFSTSEKEKTSVVEHRFQVNNLGQRDVPVSINFWVPIELKGEAVWTVMVSHPQNPLTQCYRNRLKPTQFDLLTHMQKSPVLDCSIADCLHLRCDIPSLGILDELYFILKGNLSFGWISQTLQKKVLLLSEAEITFNTSVYSQLPGQEAFLRAQTKTVLEMYKVHNPVPLIVGSSVGGLLLLAIITAILYKAGFFKRQYKEMLEEANGQFVSDGTPTPQVAQ

Target ORF information:

RefSeq Version	NM_021334.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha X (Itgax), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021334.2

ORF Insert Sequence:

ATGAGCTGTA CCTGGATAGC CTTTCTTCTG CTGTTGGGGT TTGTTTCTTG TCTTGGCTTC 
AACTTGGATG CAGAGAAGCT GACACATTTT CACATGGACG GTGCTGAGTT CGGACACAGT 
GTGCTCCAGT ATGATAGTTC CTGGGTGGTG GTTGGAGCAC CAAAGGAAAT AAAAGCCACT 
AACCAAATAG GTGGCCTCTA CAAATGTGGC TATCACACAG GCAACTGTGA GCCCATCTCC 
CTCCAGGTGC CCCCAGAGGC TGTGAACATA TCCCTGGGCC TGTCCCTTGC TGCTGCCACC 
AACCCTTCCT GGCTGTTGGC TTGTGGTCCT ACTGTGCACC ACACATGCAG AGAGAATATA 
TACTTGACAG GGCTCTGCTT TCTACTGAGT TCATCATTCA AGCAGAGCCA GAACTTCCCA 
ACTGCACAGC AGGAGTGTCC AAAGCAAGAC CAAGACATCG TGTTCCTGAT TGATGGCTCG 
GGTAGCATCA GTTCCACAGA TTTTGAAAAA ATGCTGGACT TTGTTAAAGC TGTGATGAGC 
CAGCTTCAGA GACCTAGCAC ACGGTTCTCC CTGATGCAGT TCTCTGATTA CTTCCGAGTA 
CATTTTACTT TCAACAACTT CATCTCCACG TCAAGCCCTT TAAGTCTGTT GGGTTCTGTA 
AGGCAGCTAA GAGGGTACAC ATACACAGCC TCGGCTATCA AGCATGTCAT AACAGAACTG 
TTCACCACCC AAAGTGGAGC TCGGCAAGAT GCCACCAAGG TCCTCATTGT CATCACTGAT 
GGGAGAAAAC AAGGGGACAA CTTGAGTTAT GATAGTGTCA TCCCCATGGC AGAGGCTGCA 
AGCATCATTC GTTATGCAAT TGGGGTAGGA AAGGCCTTTT ACAATGAACA TTCCAAGCAA 
GAATTAAAGG CCATTGCATC GATGCCTTCC CATGAATACG TATTCAGCGT GGAGAACTTT 
GATGCTTTGA AGGATATTGA GAATCAGCTG AAGGAGAAGA TCTTTGCCAT TGAGGGCACA 
GAGACACCAA GCAGCAGTAC TTTTGAATTG GAGATGTCCC AGGAGGGCTT CAGTGCTGTG 
TTTACACCTG ATGGACCAGT TCTGGGGGCT GTGGGAAGCT TCAGCTGGTC TGGAGGTGCC 
TTCTTGTACC CCTCAAATAT GAGACCCACC TTCATCAACA TGTCTCAGGA GAACGAGGAC 
ATGAGGGACG CTTACCTGGG TTACTCCACC GCACTGGCCT TTTGGAAGGG GGTCCACAGC 
CTGATCCTGG GGGCCCCTCG CCACCAGCAC ACGGGGAAGG TTGTCATCTT TACCCAGGAA 
TCCAGGCACT GGAGGCCCAA GTCTGAAGTC AGAGGGACAC AGATCGGCTC CTACTTTGGG 
GCATCTCTTT GTTCTGTGGA CATGGATAGA GATGGCAGCA CTGACCTGGT CCTGATTGGA 
GTCCCCCATT ACTATGAGCA CACCCGAGGG GGGCAGGTGT CAGTGTGCCC CATGCCCGGT 
GTGGGGAGCA GGTGGCATTG TGGGACCACC CTCCATGGGG AGCAGGGCCA TCCTTGGGGC 
CGCTTTGGGG CGGCTCTGAC AGTGCTAGGG GACGTGAATG GGGACAGTCT GGCAGATGTG 
GCTATTGGTG CACCCGGAGA GGAGGAGAAC AGAGGTGCTG TCTACATATT TCATGGAGCC 
TCAAGACAGG ACATCGCTCC CTCACCCAGC CAGAGGATTT CAGCATCCCA GATCCCCTCC 
AGGATTCAAT ATTTTGGGCA GTCACTGAGT GGGGGCCAGG ACCTCACCCG GGATGGACTG 
GTGGATCTGG CCGTGGGCTC CAAGGGACGC GTGCTGCTGC TCAGGACCAG ACCAATTCTT 
AGAGTGTCAC CAACTGTTCA CTTCACACCT GCAGAGATTT CCAGGTCTGT GTTTGAGTGT 
CAGGAGCAGG TGGCCCCTGA GCAGACACTG AGTGATGCCA CTGTCTGCCT TCATATTCAT 
GAAAGCCCCA AGACCCAACT AGGTGACCTC CGAAGTACTG TCACCTTTGA CCTGGCTCTA 
GACCATGGCC GTCTGAGTAC CCGGGCCATC TTCAAGGAGA CAAAGACCCG GGCTCTGACT 
CGAGTTAAAA CTCTTGGTCT GAACAAGCAC TGTGAAAGTG TGAAGTTGCT TCTCCCGGCC 
TGTGTGGAGG ACTCAGTGAC CCCGATCACT CTTCGCCTCA ACTTTTCTCT TGTTGGTGTG 
CCCATCAGTT CCTTACAAAA TCTCCAACCC ATGCTGGCTG TAGATGACCA AACGTACTTC 
ACGGCCTCTC TTCCCTTTGA GAAGAACTGT GGAGCTGACC ACATCTGCCA GGATGACCTT 
AGTGTCGTAT TTGGCTTCCC AGACTTGAAG ACCCTGGTGG TGGGAAGTGA CCTGGAGCTG 
AATGTAGATG TGACGGTGTC TAATGATGGA GAAGATTCCT ACGGAACCAC AGTCACTCTG 
TTCTACCCCG TGGGTCTGTC CTTTAGACGA GTTGCAGAAG GCCAAGTATT CCTTCGGAAG 
AAAGAGGACC AGCAGTGGCA GCGGCGTGGG CAGCACTCCC TGCACCTCAT GTGTGACAGC 
ACCCCAGACA GGAGTCAGGG CCTCTGGAGT ACCAGCTGTA GCAGCAGACA TGTCATTTTC 
CGTGGAGGCT CACAGATGAC TTTTTTGGTA ACTTTTGACG TCTCCCCCAA AGCTGAGCTG 
GGGGACCGGC TGCTTCTAAG AGCCAGAGTG GGAAGTGAGA ATAATGTACC CGGAACCCCA 
AAAACCACCT TCCAGTTGGA GCTTCCAGTA AAATATGCGG TGTACACCAT GATCAGCAGC 
CATGACCAGT TTACCAAGTA TCTTAACTTC TCAACCTCAG AAAAGGAGAA GACCAGTGTG 
GTCGAACATA GATTCCAGGT GAATAACCTG GGCCAGAGAG ACGTGCCAGT CAGCATCAAC 
TTTTGGGTGC CCATAGAGCT GAAGGGAGAA GCTGTGTGGA CAGTGATGGT CTCCCACCCT 
CAGAACCCAC TCACCCAATG CTATCGGAAT AGACTCAAGC CAACACAATT TGACCTCCTG 
ACTCACATGC AAAAGAGTCC CGTGCTGGAC TGCTCCATCG CTGACTGCCT GCACCTCCGC 
TGTGACATCC CCTCCTTGGG CATCCTGGAT GAGCTTTACT TCATTCTGAA GGGCAACCTC 
AGCTTCGGCT GGATCAGTCA GACATTGCAG AAAAAGGTGT TGCTCCTGAG TGAGGCTGAA 
ATCACATTCA ACACATCTGT GTATTCCCAG CTGCCGGGAC AGGAGGCATT TCTGAGAGCC 
CAGACGAAGA CAGTGCTGGA GATGTATAAA GTTCACAACC CCGTCCCTCT TATCGTGGGC 
AGCTCAGTGG GAGGTCTGCT GCTGCTGGCT ATCATCACAG CTATACTTTA CAAGGCTGGT 
TTCTTCAAAC GTCAGTACAA GGAGATGTTG GAGGAAGCAA ATGGACAGTT TGTCTCAGAC 
GGGACCCCAA CTCCCCAGGT TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel microglial subset plays a key role in myelinogenesis in developing brain.
The EMBO journal36(22)3292-3308(2017 11)
Wlodarczyk A,Holtman IR,Krueger M,Yogev N,Bruttger J,Khorooshi R,Benmamar-Badel A,de Boer-Bergsma JJ,Martin NA,Karram K,Kramer I,Boddeke EW,Waisman A,Eggen BJ,Owens T

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Itgax cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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