Dnaja1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaja1
Entrez Gene ID	15502
Full Name	DnaJ heat shock protein family (Hsp40) member A1
Synonyms	HSJ-2,Hsj2,Nedd7
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member A1 Names dnaJ homolog subfamily A member 1 DnaJ (Hsp40) homolog, subfamily A, member 1 dnaJ protein homolog 2 heat shock 40 kDa protein 4 heat shock protein J2 heat shock protein, Dnaj-like 2 neural precursor cell expressed, developmentally down-regulated gene 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A5
Summary	The protein encoded by this gene is a member of the DnaJ family, whose members act as cochaperones of heat shock protein 70. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. Mice deficient for this gene display reduced levels of activation‐induced deaminase, an enzyme that deaminates deoxycytidine at the immunoglobulin genes during immune responses. In addition, mice lacking this gene exhibit severe defects in spermatogenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164672.2	NP_001158144.1	dnaJ homolog subfamily A member 1
NM_001164671.2	NP_001158143.1	dnaJ homolog subfamily A member 1
NM_008298.6	NP_032324.1	dnaJ homolog subfamily A member 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DNAJA1	NP_001233463.1
Bos taurus (cattle)	DNAJA1	NP_001015637.1
Rattus norvegicus (Norway rat)	Dnaja1	NP_075223.1
Gallus gallus (chicken)	DNAJA1	NP_001012963.1
Danio rerio (zebrafish)	dnaja1	NP_955956.1
Homo sapiens (human)	DNAJA1	NP_001530.1
Canis lupus familiaris (dog)	DNAJA1	NP_001239072.1
Xenopus tropicalis (tropical clawed frog)	dnaja1	NP_001011012.1
	DNAJA1	XP_001101766.2
Mus musculus (house mouse)	Dnaja1	NP_001158143.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

HECT E3 Ubiquitin Ligase Itch Functions as a Novel Negative Regulator of Gli-Similar 3 (Glis3) Transcriptional Activity.
ZeRuth GT, Williams JG, Cole YC, Jetten AM
PloS one10(7)e0131303(2015)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
Slater LH, Hett EC, Clatworthy AE, Mark KG, Hung DT
Proceedings of the National Academy of Sciences of the United States of America110(24)9932-7(2013 Jun)

Multiple molecules of Hsc70 and a dimer of DjA1 independently bind to an unfolded protein.
Terada K, Oike Y
The Journal of biological chemistry285(22)16789-97(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DnaJa1 farnesylation is required to maintain, and farnesyltransferase inhibition reduces, AID protein levels in B cells. Thus, DnaJa1 is a limiting factor that plays a non-redundant role in the functional stabilization of AID
Title: Optimal functional levels of activation-induced deaminase specifically require the Hsp40 DnaJa1.

Hsc70 and a dimer of DjA1 independently bind to an unfolded protein
Title: Multiple molecules of Hsc70 and a dimer of DjA1 independently bind to an unfolded protein.

Results indicate that DjA1 and DjB4 of subfamilies A and B of human Hsp40 have different quaternary structures and chaperone functions.
Title: Low resolution structural study of two human HSP40 chaperones in solution. DJA1 from subfamily A and DJB4 from subfamily B have different quaternary structures.

loss of DjA1 in led to severe defects in spermatogenesis that involve aberrant androgen signaling
Title: A type I DnaJ homolog, DjA1, regulates androgen receptor signaling and spermatogenesis.

DnaJ 1 protein expression is down regulated in the spinal cord and testis of wobbler mouse and precedes motor neuron degeneration.
Title: Early defect in the expression of mouse sperm DNAJ 1, a member of the DNAJ/heat shock protein 40 chaperone protein family, in the spinal cord of the wobbler mouse, a murine model of motoneuronal degeneration.

The following Dnaja1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaja1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01684	NM_001164671.2 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01684	NM_008298.6 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01684	NM_001164672.2 Latest version!	Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaja1 ( NM_001164672.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158144.1 Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu01684
Clone ID Related Accession (Same CDS sequence)	NM_001164672.2 , NM_001164671.2 , NM_008298.6
Accession Version	NM_001164672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily A member 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK617936.1, AK145963.1, AK083046.1 and BP766105.1. On Sep 26, 2015 this sequence version replaced NM_001164672.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.3998048.1, SRR7345562.1576719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAAG AAACCACTTA CTACGATGTT TTGGGGGTAA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA GAATCCAAAT 
GAAGGGGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAAAAA 
AGGGAACTAT ATGATAAAGG AGGGGAGCAG GCGATTAAAG AGGGCGGAGC AGGTGGTGGT 
TTTGGCTCAC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG AATGCAAAGA 
GAAAGGAGAG GTAAAAATGT TGTTCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GAAAACTGGC TCTGCAAAAG AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACAGG TATGCAGATA 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCAAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAA 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGCGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAACATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158144.1
CDS	83..1276
Translation	MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

Target ORF information:

RefSeq Version	NM_001164672.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164672.2

ORF Insert Sequence:

ATGGTGAAAG AAACCACTTA CTACGATGTT TTGGGGGTAA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA GAATCCAAAT 
GAAGGGGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAAAAA 
AGGGAACTAT ATGATAAAGG AGGGGAGCAG GCGATTAAAG AGGGCGGAGC AGGTGGTGGT 
TTTGGCTCAC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG AATGCAAAGA 
GAAAGGAGAG GTAAAAATGT TGTTCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GAAAACTGGC TCTGCAAAAG AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACAGG TATGCAGATA 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCAAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAA 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGCGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAACATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaja1 ( NM_001164671.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158143.1 Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01684
Clone ID Related Accession (Same CDS sequence)	NM_001164672.2 , NM_001164671.2 , NM_008298.6
Accession Version	NM_001164671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily A member 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY245852.1, CN669546.1, AK145963.1, AK083046.1 and BP766105.1. On Sep 26, 2015 this sequence version replaced NM_001164671.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.185994.1, SRR1660817.111619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAAG AAACCACTTA CTACGATGTT TTGGGGGTAA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA GAATCCAAAT 
GAAGGGGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAAAAA 
AGGGAACTAT ATGATAAAGG AGGGGAGCAG GCGATTAAAG AGGGCGGAGC AGGTGGTGGT 
TTTGGCTCAC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG AATGCAAAGA 
GAAAGGAGAG GTAAAAATGT TGTTCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GAAAACTGGC TCTGCAAAAG AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACAGG TATGCAGATA 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCAAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAA 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGCGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAACATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158143.1
CDS	226..1419
Translation	MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

Target ORF information:

RefSeq Version	NM_001164671.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164671.2

ORF Insert Sequence:

ATGGTGAAAG AAACCACTTA CTACGATGTT TTGGGGGTAA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA GAATCCAAAT 
GAAGGGGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAAAAA 
AGGGAACTAT ATGATAAAGG AGGGGAGCAG GCGATTAAAG AGGGCGGAGC AGGTGGTGGT 
TTTGGCTCAC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG AATGCAAAGA 
GAAAGGAGAG GTAAAAATGT TGTTCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GAAAACTGGC TCTGCAAAAG AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACAGG TATGCAGATA 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCAAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAA 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGCGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAACATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaja1 ( NM_008298.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032324.1 Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01684
Clone ID Related Accession (Same CDS sequence)	NM_001164672.2 , NM_001164671.2 , NM_008298.6
Accession Version	NM_008298.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Mus musculus(house mouse)
Product	dnaJ homolog subfamily A member 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB574002.1, AK145963.1, AK083046.1 and BP766105.1. On Sep 26, 2015 this sequence version replaced NM_008298.5. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083046.1, SRR1660811.198521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAAG AAACCACTTA CTACGATGTT TTGGGGGTAA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA GAATCCAAAT 
GAAGGGGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAAAAA 
AGGGAACTAT ATGATAAAGG AGGGGAGCAG GCGATTAAAG AGGGCGGAGC AGGTGGTGGT 
TTTGGCTCAC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG AATGCAAAGA 
GAAAGGAGAG GTAAAAATGT TGTTCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GAAAACTGGC TCTGCAAAAG AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACAGG TATGCAGATA 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCAAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAA 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGCGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAACATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032324.1
CDS	177..1370
Translation	MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS

Target ORF information:

RefSeq Version	NM_008298.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus DnaJ heat shock protein family (Hsp40) member A1 (Dnaja1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008298.6

ORF Insert Sequence:

ATGGTGAAAG AAACCACTTA CTACGATGTT TTGGGGGTAA AACCCAATGC CACCCAGGAA 
GAATTGAAAA AGGCATATAG AAAATTGGCC TTGAAGTACC ACCCTGATAA GAATCCAAAT 
GAAGGGGAAA AGTTTAAACA GATTTCTCAA GCTTATGAAG TTCTTGCTGA TTCCAAAAAA 
AGGGAACTAT ATGATAAAGG AGGGGAGCAG GCGATTAAAG AGGGCGGAGC AGGTGGTGGT 
TTTGGCTCAC CCATGGATAT CTTTGATATG TTCTTTGGAG GAGGAGGAAG AATGCAAAGA 
GAAAGGAGAG GTAAAAATGT TGTTCATCAG CTCTCAGTGA CCTTAGAAGA CTTATATAAT 
GGTGCAACAA GAAAACTGGC TCTGCAAAAG AATGTGATTT GTGACAAATG TGAAGGCCGA 
GGTGGTAAGA AAGGAGCAGT AGAGTGCTGT CCCAACTGCC GGGGGACAGG TATGCAGATA 
AGGATTCATC AGATTGGACC AGGAATGGTT CAGCAAATTC AGTCAGTGTG CATGGAGTGC 
CAGGGTCATG GAGAACGCAT CAGTCCAAAA GACAGATGTA AAAGCTGCAA TGGAAGAAAA 
ATAGTTCGAG AGAAGAAAAT TTTAGAAGTT CATATTGATA AAGGCATGAA AGATGGTCAG 
AAGATAACAT TCCACGGTGA AGGAGACCAA GAACCAGGAC TGGAGCCAGG AGATATTATC 
ATTGTGTTAG ATCAGAAGGA CCATGCTGTT TTTACAAGGC GAGGAGAAGA CCTTTTCATG 
TGTATGGACA TACAGCTGGT TGAAGCATTG TGCGGCTTCC AAAAGCCAAT ATCTACTCTT 
GACAACCGAA CCATAGTCAT CACCTCTCAT CCAGGTCAGA TTGTCAAGCA TGGGGATATA 
AAATGTGTGC TAAATGAAGG TATGCCAATA TACCGTCGGC CATATGAAAA GGGACGTCTA 
ATCATTGAGT TTAAGGTAAA CTTTCCTGAA AATGGCTTTC TCTCTCCTGA TAAACTCTCT 
TTGCTGGAAA AACTCCTTCC TGAAAGGAAG GAAGTAGAAG AGACTGATGA AATGGATCAG 
GTAGAACTGG TGGACTTTGA TCCAAATCAG GAAAGACGGC GTCATTATAA TGGAGAAGCG 
TATGAGGATG ATGAACATCA CCCCAGAGGT GGCGTTCAGT GTCAGACCTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

HECT E3 Ubiquitin Ligase Itch Functions as a Novel Negative Regulator of Gli-Similar 3 (Glis3) Transcriptional Activity.
PloS one10(7)e0131303(2015)
ZeRuth GT,Williams JG,Cole YC,Jetten AM

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
Proceedings of the National Academy of Sciences of the United States of America110(24)9932-7(2013 Jun)
Slater LH,Hett EC,Clatworthy AE,Mark KG,Hung DT

Multiple molecules of Hsc70 and a dimer of DjA1 independently bind to an unfolded protein.
The Journal of biological chemistry285(22)16789-97(2010 May)
Terada K,Oike Y

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Dnaja1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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