Hsf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsf2
Entrez Gene ID	15500
Full Name	heat shock factor 2
Synonyms	AI661205
General protein information	Preferred Names heat shock factor 2 Names heat shock factor protein 2 HSF 2 HSTF 2 heat shock transcription factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512564.2	XP_006512627.1	heat shock factor protein 2 isoform X1
XM_006512565.3	XP_006512628.1	heat shock factor protein 2 isoform X2
XM_006512565.4	XP_006512628.1	heat shock factor protein 2 isoform X2
NM_008297.3	NP_032323.3	heat shock factor protein 2
XM_006512564.3	XP_006512627.1	heat shock factor protein 2 isoform X1

Pathways from BioSystems

Homologies

	HSF2	XP_001108944.2
Gallus gallus (chicken)	HSF2	NP_001161236.1
Pan troglodytes (chimpanzee)	HSF2	XP_003311472.1
Bos taurus (cattle)	HSF2	NP_001076874.1
Xenopus tropicalis (tropical clawed frog)	hsf2	XP_002937261.1
Canis lupus familiaris (dog)	HSF2	XP_005615766.1
Mus musculus (house mouse)	Hsf2	NP_032323.3
Danio rerio (zebrafish)	hsf2	NP_571942.1
Homo sapiens (human)	HSF2	NP_004497.1
Rattus norvegicus (Norway rat)	Hsf2	NP_113882.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation.
Borowiec AS, Sion B, Chalmel F, D Rolland A, Lemonnier L, De Clerck T, Bokhobza A, Derouiche S, Dewailly E, Slomianny C, Mauduit C, Benahmed M, Roudbaraki M, J?gou B, Prevarskaya N, Bidaux G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(9)3155-70(2016 09)

Set1/MLL complex is indispensable for the transcriptional ability of heat shock transcription factor 2.
Hayashida N
Biochemical and biophysical research communications467(4)805-12(2015 Nov)

HSF2 autoregulates its own transcription.
Park SM, Kim SA, Ahn SG
International journal of molecular medicine36(4)1173-9(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Set1/MLL complex is indispensable for the transcriptional ability of HSF2.
Title: Set1/MLL complex is indispensable for the transcriptional ability of heat shock transcription factor 2.

the findings of our studys suggest that HSF2 contributes to its own expression by forming a negative autoregulatory loop.
Title: HSF2 autoregulates its own transcription.

Authors have concluded that at the physiological temperature HSF1 and HSF2 cooperate in spermatogenic cells. However, temperature elevation causes remodeling of chromatin binding and interactions between HSFs are disrupted.
Title: Crosstalk between HSF1 and HSF2 during the heat shock response in mouse testes.

Thus, HSF2, as a sensor for alcohol stress in the fetal brain, acts as a mediator of the neuronal migration defects associated with fetal alcohol syndrome.
Title: Heat shock factor 2 is a stress-responsive mediator of neuronal migration defects in models of fetal alcohol syndrome.

Results suggest that relative amounts of each heat shock factors HSF1 and HSF2 isoforms quantitatively determine the cellular level of the proteotoxic stress response.
Title: Unraveling complex interplay between heat shock factor 1 and 2 splicing isoforms.

The following Hsf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43737	XM_006512565.4 Latest version!	Mus musculus heat shock factor 2 (Hsf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu01683	NM_008297.3 Latest version!	Mus musculus heat shock factor 2 (Hsf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00
OMu43736	XM_006512564.3 Latest version!	Mus musculus heat shock factor 2 (Hsf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu43737	XM_006512565.3	Mus musculus heat shock factor 2 (Hsf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu43736	XM_006512564.2	Mus musculus heat shock factor 2 (Hsf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsf2 ( XM_006512564.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512627.1 Mus musculus heat shock factor 2 (Hsf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43736
Clone ID Related Accession (Same CDS sequence)	XM_006512564.2 , XM_006512564.3
Accession Version	XM_006512564.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	heat shock factor protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006512564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGA GTTCCAACGT GCCGGCTTTC CTCAGCAAGC TGTGGACGCT TGTGGAGGAA 
ACCCACACCA ACGAGTTCAT CACCTGGAGT CAGAATGGAC AAAGTTTTCT GGTCTTGGAT 
GAGCAAAGAT TTGCAAAGGA AATTCTTCCT AAGTACTTCA AACACAATAA CATGGCGAGC 
TTTGTGAGAC AACTAAATAT GTATGGCTTC CGAAAAGTAG TGCATATCGA ATCTGGAATT 
ATCAAACAGG AAAGAGATGG CCCTGTTGAA TTTCAGCATC CTTATTTCAA GCAAGGCCAG 
GATGACCTGT TGGAGAACAT TAAAAGGAAG GTTTCATCTT CAAAACCAGA GGAAAATAAA 
ATTCGTCAGG AAGATTTAAC AAAAATTATT AGTAGTGCTC AGAAGGTTCA AATAAAACAA 
GAAACTATTG AGTCCAGGCT TTCAGAATTA AAAAGTGAGA ATGAATCCCT TTGGAAGGAG 
GTGTCAGAAC TAAGAGCAAA GCATGCCCAG CAGCAACAAG TTATTCGGAA GATTGTCCAG 
TTTATTGTTA CATTGGTTCA GAATAATCAA CTTGTGAGTT TAAAACGTAA AAGGCCTCTA 
CTTCTAAACA CAAATGGAGC CCCAAAGAAG AATCTATATC AGCACATAGT CAAAGAACCA 
ACTGATAATC ACCATCATAA AGTTCCACAC AGCAGGACTG AAGGTTTAAA GTCAAGAGAA 
CGGATTTCAG ATGACATAAT TATTTATGAT GTTACTGACG ATAATGTGGA TGAAGAAAAT 
ATTCCAGTTA TTCCAGAAAC AAATGAGGAT GTTGTAGTGG ATTCCTCCAA CCAGTATCCT 
GACATTGTCA TTGTTGAAGA TGACAACGAG GATGAGTATG CTCCTGTCAT TCAGAGTGGA 
GAGCAGAGTG AACCAGCCAG AGAACCCTTA CGTGTGGGGA GTGCTGGCAG CAGCAGCCCT 
CTCATGTCTA GTGCTGTCCA GCTAAACGGC TCCTCCAGTC TGACCTCAGA AGACCCTGTG 
ACCATGATGG ACTCCATTCT GAATGACAAC ATTAACCTGT TAGGAAAGGT TGAGCTGTTG 
GATTACCTTG ACAGTATTGA TTGCAGTTTA GAGGACTTCC AAGCTATGCT CTCAGGAAGA 
CAGTTTAGCA TAGACCCAGA TCTTCTGGTT GATCTTTTCA CTAGTTCTGT GCAGATGAAT 
CCCACAGATA ACATCAATAA TACAAAATCT GAGAATAAGG GACTAGAAGC TACCAAGAGC 
AGTGTTGTTC AACATGTGTC AGAAGAGGGA AGAAAATCTA AATCCAAGCC AGACAAACAA 
CTTATCCAGT ATACTGCCTT TCCACTTCTT GCATTCCTGG ATGGGAACTC TGCATCTGCT 
ATTGAACAGG GGAGTACAAC TGCATCGTCA GAAGTTGTGC CTTCTGTAGA TAAACCCATA 
GAAGTCGATG AGCTCCTGGA TAGCAGCCTG GATCCAGAAC CGACCCAGAG TAAGCTTGTC 
CGCCTGGAAC CATTGACTGA AGCGGAAGCT AGTGAAGCCA CACTCTTCTA TTTATGTGAA 
CTTGCTCCTG CACCTCTGGA TAGTGATATG CCGCTTTTAG ATAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512627.1
CDS	323..1930
Translation	MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDNINNTKSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

Target ORF information:

RefSeq Version	XM_006512564.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock factor 2 (Hsf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512564.2

ORF Insert Sequence:

ATGAAGCAGA GTTCCAACGT GCCGGCTTTC CTCAGCAAGC TGTGGACGCT TGTGGAGGAA 
ACCCACACCA ACGAGTTCAT CACCTGGAGT CAGAATGGAC AAAGTTTTCT GGTCTTGGAT 
GAGCAAAGAT TTGCAAAGGA AATTCTTCCT AAGTACTTCA AACACAATAA CATGGCGAGC 
TTTGTGAGAC AACTAAATAT GTATGGCTTC CGAAAAGTAG TGCATATCGA ATCTGGAATT 
ATCAAACAGG AAAGAGATGG CCCTGTTGAA TTTCAGCATC CTTATTTCAA GCAAGGCCAG 
GATGACCTGT TGGAGAACAT TAAAAGGAAG GTTTCATCTT CAAAACCAGA GGAAAATAAA 
ATTCGTCAGG AAGATTTAAC AAAAATTATT AGTAGTGCTC AGAAGGTTCA AATAAAACAA 
GAAACTATTG AGTCCAGGCT TTCAGAATTA AAAAGTGAGA ATGAATCCCT TTGGAAGGAG 
GTGTCAGAAC TAAGAGCAAA GCATGCCCAG CAGCAACAAG TTATTCGGAA GATTGTCCAG 
TTTATTGTTA CATTGGTTCA GAATAATCAA CTTGTGAGTT TAAAACGTAA AAGGCCTCTA 
CTTCTAAACA CAAATGGAGC CCCAAAGAAG AATCTATATC AGCACATAGT CAAAGAACCA 
ACTGATAATC ACCATCATAA AGTTCCACAC AGCAGGACTG AAGGTTTAAA GTCAAGAGAA 
CGGATTTCAG ATGACATAAT TATTTATGAT GTTACTGACG ATAATGTGGA TGAAGAAAAT 
ATTCCAGTTA TTCCAGAAAC AAATGAGGAT GTTGTAGTGG ATTCCTCCAA CCAGTATCCT 
GACATTGTCA TTGTTGAAGA TGACAACGAG GATGAGTATG CTCCTGTCAT TCAGAGTGGA 
GAGCAGAGTG AACCAGCCAG AGAACCCTTA CGTGTGGGGA GTGCTGGCAG CAGCAGCCCT 
CTCATGTCTA GTGCTGTCCA GCTAAACGGC TCCTCCAGTC TGACCTCAGA AGACCCTGTG 
ACCATGATGG ACTCCATTCT GAATGACAAC ATTAACCTGT TAGGAAAGGT TGAGCTGTTG 
GATTACCTTG ACAGTATTGA TTGCAGTTTA GAGGACTTCC AAGCTATGCT CTCAGGAAGA 
CAGTTTAGCA TAGACCCAGA TCTTCTGGTT GATCTTTTCA CTAGTTCTGT GCAGATGAAT 
CCCACAGATA ACATCAATAA TACAAAATCT GAGAATAAGG GACTAGAAGC TACCAAGAGC 
AGTGTTGTTC AACATGTGTC AGAAGAGGGA AGAAAATCTA AATCCAAGCC AGACAAACAA 
CTTATCCAGT ATACTGCCTT TCCACTTCTT GCATTCCTGG ATGGGAACTC TGCATCTGCT 
ATTGAACAGG GGAGTACAAC TGCATCGTCA GAAGTTGTGC CTTCTGTAGA TAAACCCATA 
GAAGTCGATG AGCTCCTGGA TAGCAGCCTG GATCCAGAAC CGACCCAGAG TAAGCTTGTC 
CGCCTGGAAC CATTGACTGA AGCGGAAGCT AGTGAAGCCA CACTCTTCTA TTTATGTGAA 
CTTGCTCCTG CACCTCTGGA TAGTGATATG CCGCTTTTAG ATAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf2 ( XM_006512565.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512628.1 Mus musculus heat shock factor 2 (Hsf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43737
Clone ID Related Accession (Same CDS sequence)	XM_006512565.3 , XM_006512565.4
Accession Version	XM_006512565.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	heat shock factor protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCT TTGTGAGACA ACTAAATATG TATGGCTTCC GAAAAGTAGT GCATATCGAA 
TCTGGAATTA TCAAACAGGA AAGAGATGGC CCTGTTGAAT TTCAGCATCC TTATTTCAAG 
CAAGGCCAGG ATGACCTGTT GGAGAACATT AAAAGGAAGG TTTCATCTTC AAAACCAGAG 
GAAAATAAAA TTCGTCAGGA AGATTTAACA AAAATTATTA GTAGTGCTCA GAAGGTTCAA 
ATAAAACAAG AAACTATTGA GTCCAGGCTT TCAGAATTAA AAAGTGAGAA TGAATCCCTT 
TGGAAGGAGG TGTCAGAACT AAGAGCAAAG CATGCCCAGC AGCAACAAGT TATTCGGAAG 
ATTGTCCAGT TTATTGTTAC ATTGGTTCAG AATAATCAAC TTGTGAGTTT AAAACGTAAA 
AGGCCTCTAC TTCTAAACAC AAATGGAGCC CCAAAGAAGA ATCTATATCA GCACATAGTC 
AAAGAACCAA CTGATAATCA CCATCATAAA GTTCCACACA GCAGGACTGA AGGTTTAAAG 
TCAAGAGAAC GGATTTCAGA TGACATAATT ATTTATGATG TTACTGACGA TAATGTGGAT 
GAAGAAAATA TTCCAGTTAT TCCAGAAACA AATGAGGATG TTGTAGTGGA TTCCTCCAAC 
CAGTATCCTG ACATTGTCAT TGTTGAAGAT GACAACGAGG ATGAGTATGC TCCTGTCATT 
CAGAGTGGAG AGCAGAGTGA ACCAGCCAGA GAACCCTTAC GTGTGGGGAG TGCTGGCAGC 
AGCAGCCCTC TCATGTCTAG TGCTGTCCAG CTAAACGGCT CCTCCAGTCT GACCTCAGAA 
GACCCTGTGA CCATGATGGA CTCCATTCTG AATGACAACA TTAACCTGTT AGGAAAGGTT 
GAGCTGTTGG ATTACCTTGA CAGTATTGAT TGCAGTTTAG AGGACTTCCA AGCTATGCTC 
TCAGGAAGAC AGTTTAGCAT AGACCCAGAT CTTCTGGTTG ATCTTTTCAC TAGTTCTGTG 
CAGATGAATC CCACAGATAA CATCAATAAT ACAAAATCTG AGAATAAGGG ACTAGAAGCT 
ACCAAGAGCA GTGTTGTTCA ACATGTGTCA GAAGAGGGAA GAAAATCTAA ATCCAAGCCA 
GACAAACAAC TTATCCAGTA TACTGCCTTT CCACTTCTTG CATTCCTGGA TGGGAACTCT 
GCATCTGCTA TTGAACAGGG GAGTACAACT GCATCGTCAG AAGTTGTGCC TTCTGTAGAT 
AAACCCATAG AAGTCGATGA GCTCCTGGAT AGCAGCCTGG ATCCAGAACC GACCCAGAGT 
AAGCTTGTCC GCCTGGAACC ATTGACTGAA GCGGAAGCTA GTGAAGCCAC ACTCTTCTAT 
TTATGTGAAC TTGCTCCTGC ACCTCTGGAT AGTGATATGC CGCTTTTAGA TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512628.1
CDS	4276..5712
Translation	MASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDNINNTKSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

Target ORF information:

RefSeq Version	XM_006512565.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock factor 2 (Hsf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512565.3

ORF Insert Sequence:

ATGGCGAGCT TTGTGAGACA ACTAAATATG TATGGCTTCC GAAAAGTAGT GCATATCGAA 
TCTGGAATTA TCAAACAGGA AAGAGATGGC CCTGTTGAAT TTCAGCATCC TTATTTCAAG 
CAAGGCCAGG ATGACCTGTT GGAGAACATT AAAAGGAAGG TTTCATCTTC AAAACCAGAG 
GAAAATAAAA TTCGTCAGGA AGATTTAACA AAAATTATTA GTAGTGCTCA GAAGGTTCAA 
ATAAAACAAG AAACTATTGA GTCCAGGCTT TCAGAATTAA AAAGTGAGAA TGAATCCCTT 
TGGAAGGAGG TGTCAGAACT AAGAGCAAAG CATGCCCAGC AGCAACAAGT TATTCGGAAG 
ATTGTCCAGT TTATTGTTAC ATTGGTTCAG AATAATCAAC TTGTGAGTTT AAAACGTAAA 
AGGCCTCTAC TTCTAAACAC AAATGGAGCC CCAAAGAAGA ATCTATATCA GCACATAGTC 
AAAGAACCAA CTGATAATCA CCATCATAAA GTTCCACACA GCAGGACTGA AGGTTTAAAG 
TCAAGAGAAC GGATTTCAGA TGACATAATT ATTTATGATG TTACTGACGA TAATGTGGAT 
GAAGAAAATA TTCCAGTTAT TCCAGAAACA AATGAGGATG TTGTAGTGGA TTCCTCCAAC 
CAGTATCCTG ACATTGTCAT TGTTGAAGAT GACAACGAGG ATGAGTATGC TCCTGTCATT 
CAGAGTGGAG AGCAGAGTGA ACCAGCCAGA GAACCCTTAC GTGTGGGGAG TGCTGGCAGC 
AGCAGCCCTC TCATGTCTAG TGCTGTCCAG CTAAACGGCT CCTCCAGTCT GACCTCAGAA 
GACCCTGTGA CCATGATGGA CTCCATTCTG AATGACAACA TTAACCTGTT AGGAAAGGTT 
GAGCTGTTGG ATTACCTTGA CAGTATTGAT TGCAGTTTAG AGGACTTCCA AGCTATGCTC 
TCAGGAAGAC AGTTTAGCAT AGACCCAGAT CTTCTGGTTG ATCTTTTCAC TAGTTCTGTG 
CAGATGAATC CCACAGATAA CATCAATAAT ACAAAATCTG AGAATAAGGG ACTAGAAGCT 
ACCAAGAGCA GTGTTGTTCA ACATGTGTCA GAAGAGGGAA GAAAATCTAA ATCCAAGCCA 
GACAAACAAC TTATCCAGTA TACTGCCTTT CCACTTCTTG CATTCCTGGA TGGGAACTCT 
GCATCTGCTA TTGAACAGGG GAGTACAACT GCATCGTCAG AAGTTGTGCC TTCTGTAGAT 
AAACCCATAG AAGTCGATGA GCTCCTGGAT AGCAGCCTGG ATCCAGAACC GACCCAGAGT 
AAGCTTGTCC GCCTGGAACC ATTGACTGAA GCGGAAGCTA GTGAAGCCAC ACTCTTCTAT 
TTATGTGAAC TTGCTCCTGC ACCTCTGGAT AGTGATATGC CGCTTTTAGA TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf2 ( XM_006512565.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512628.1 Mus musculus heat shock factor 2 (Hsf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43737
Clone ID Related Accession (Same CDS sequence)	XM_006512565.3 , XM_006512565.4
Accession Version	XM_006512565.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	heat shock factor protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512565.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCT TTGTGAGACA ACTAAATATG TATGGCTTCC GAAAAGTAGT GCATATCGAA 
TCTGGAATTA TCAAACAGGA AAGAGATGGC CCTGTTGAAT TTCAGCATCC TTATTTCAAG 
CAAGGCCAGG ATGACCTGTT GGAGAACATT AAAAGGAAGG TTTCATCTTC AAAACCAGAG 
GAAAATAAAA TTCGTCAGGA AGATTTAACA AAAATTATTA GTAGTGCTCA GAAGGTTCAA 
ATAAAACAAG AAACTATTGA GTCCAGGCTT TCAGAATTAA AAAGTGAGAA TGAATCCCTT 
TGGAAGGAGG TGTCAGAACT AAGAGCAAAG CATGCCCAGC AGCAACAAGT TATTCGGAAG 
ATTGTCCAGT TTATTGTTAC ATTGGTTCAG AATAATCAAC TTGTGAGTTT AAAACGTAAA 
AGGCCTCTAC TTCTAAACAC AAATGGAGCC CCAAAGAAGA ATCTATATCA GCACATAGTC 
AAAGAACCAA CTGATAATCA CCATCATAAA GTTCCACACA GCAGGACTGA AGGTTTAAAG 
TCAAGAGAAC GGATTTCAGA TGACATAATT ATTTATGATG TTACTGACGA TAATGTGGAT 
GAAGAAAATA TTCCAGTTAT TCCAGAAACA AATGAGGATG TTGTAGTGGA TTCCTCCAAC 
CAGTATCCTG ACATTGTCAT TGTTGAAGAT GACAACGAGG ATGAGTATGC TCCTGTCATT 
CAGAGTGGAG AGCAGAGTGA ACCAGCCAGA GAACCCTTAC GTGTGGGGAG TGCTGGCAGC 
AGCAGCCCTC TCATGTCTAG TGCTGTCCAG CTAAACGGCT CCTCCAGTCT GACCTCAGAA 
GACCCTGTGA CCATGATGGA CTCCATTCTG AATGACAACA TTAACCTGTT AGGAAAGGTT 
GAGCTGTTGG ATTACCTTGA CAGTATTGAT TGCAGTTTAG AGGACTTCCA AGCTATGCTC 
TCAGGAAGAC AGTTTAGCAT AGACCCAGAT CTTCTGGTTG ATCTTTTCAC TAGTTCTGTG 
CAGATGAATC CCACAGATAA CATCAATAAT ACAAAATCTG AGAATAAGGG ACTAGAAGCT 
ACCAAGAGCA GTGTTGTTCA ACATGTGTCA GAAGAGGGAA GAAAATCTAA ATCCAAGCCA 
GACAAACAAC TTATCCAGTA TACTGCCTTT CCACTTCTTG CATTCCTGGA TGGGAACTCT 
GCATCTGCTA TTGAACAGGG GAGTACAACT GCATCGTCAG AAGTTGTGCC TTCTGTAGAT 
AAACCCATAG AAGTCGATGA GCTCCTGGAT AGCAGCCTGG ATCCAGAACC GACCCAGAGT 
AAGCTTGTCC GCCTGGAACC ATTGACTGAA GCGGAAGCTA GTGAAGCCAC ACTCTTCTAT 
TTATGTGAAC TTGCTCCTGC ACCTCTGGAT AGTGATATGC CGCTTTTAGA TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512628.1
CDS	4281..5717
Translation	MASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDNINNTKSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

Target ORF information:

RefSeq Version	XM_006512565.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock factor 2 (Hsf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512565.4

ORF Insert Sequence:

ATGGCGAGCT TTGTGAGACA ACTAAATATG TATGGCTTCC GAAAAGTAGT GCATATCGAA 
TCTGGAATTA TCAAACAGGA AAGAGATGGC CCTGTTGAAT TTCAGCATCC TTATTTCAAG 
CAAGGCCAGG ATGACCTGTT GGAGAACATT AAAAGGAAGG TTTCATCTTC AAAACCAGAG 
GAAAATAAAA TTCGTCAGGA AGATTTAACA AAAATTATTA GTAGTGCTCA GAAGGTTCAA 
ATAAAACAAG AAACTATTGA GTCCAGGCTT TCAGAATTAA AAAGTGAGAA TGAATCCCTT 
TGGAAGGAGG TGTCAGAACT AAGAGCAAAG CATGCCCAGC AGCAACAAGT TATTCGGAAG 
ATTGTCCAGT TTATTGTTAC ATTGGTTCAG AATAATCAAC TTGTGAGTTT AAAACGTAAA 
AGGCCTCTAC TTCTAAACAC AAATGGAGCC CCAAAGAAGA ATCTATATCA GCACATAGTC 
AAAGAACCAA CTGATAATCA CCATCATAAA GTTCCACACA GCAGGACTGA AGGTTTAAAG 
TCAAGAGAAC GGATTTCAGA TGACATAATT ATTTATGATG TTACTGACGA TAATGTGGAT 
GAAGAAAATA TTCCAGTTAT TCCAGAAACA AATGAGGATG TTGTAGTGGA TTCCTCCAAC 
CAGTATCCTG ACATTGTCAT TGTTGAAGAT GACAACGAGG ATGAGTATGC TCCTGTCATT 
CAGAGTGGAG AGCAGAGTGA ACCAGCCAGA GAACCCTTAC GTGTGGGGAG TGCTGGCAGC 
AGCAGCCCTC TCATGTCTAG TGCTGTCCAG CTAAACGGCT CCTCCAGTCT GACCTCAGAA 
GACCCTGTGA CCATGATGGA CTCCATTCTG AATGACAACA TTAACCTGTT AGGAAAGGTT 
GAGCTGTTGG ATTACCTTGA CAGTATTGAT TGCAGTTTAG AGGACTTCCA AGCTATGCTC 
TCAGGAAGAC AGTTTAGCAT AGACCCAGAT CTTCTGGTTG ATCTTTTCAC TAGTTCTGTG 
CAGATGAATC CCACAGATAA CATCAATAAT ACAAAATCTG AGAATAAGGG ACTAGAAGCT 
ACCAAGAGCA GTGTTGTTCA ACATGTGTCA GAAGAGGGAA GAAAATCTAA ATCCAAGCCA 
GACAAACAAC TTATCCAGTA TACTGCCTTT CCACTTCTTG CATTCCTGGA TGGGAACTCT 
GCATCTGCTA TTGAACAGGG GAGTACAACT GCATCGTCAG AAGTTGTGCC TTCTGTAGAT 
AAACCCATAG AAGTCGATGA GCTCCTGGAT AGCAGCCTGG ATCCAGAACC GACCCAGAGT 
AAGCTTGTCC GCCTGGAACC ATTGACTGAA GCGGAAGCTA GTGAAGCCAC ACTCTTCTAT 
TTATGTGAAC TTGCTCCTGC ACCTCTGGAT AGTGATATGC CGCTTTTAGA TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf2 ( NM_008297.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032323.3 Mus musculus heat shock factor 2 (Hsf2), mRNA.

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CloneID	OMu01683
Clone ID Related Accession (Same CDS sequence)	NM_008297.3
Accession Version	NM_008297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	heat shock factor protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK053976.1, AK052270.1 and BB823041.1. On Apr 11, 2009 this sequence version replaced NM_008297.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK052270.1, BC018414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCAGA GTTCCAACGT GCCGGCTTTC CTCAGCAAGC TGTGGACGCT TGTGGAGGAA 
ACCCACACCA ACGAGTTCAT CACCTGGAGT CAGAATGGAC AAAGTTTTCT GGTCTTGGAT 
GAGCAAAGAT TTGCAAAGGA AATTCTTCCT AAGTACTTCA AACACAATAA CATGGCGAGC 
TTTGTGAGAC AACTAAATAT GTATGGCTTC CGAAAAGTAG TGCATATCGA ATCTGGAATT 
ATCAAACAGG AAAGAGATGG CCCTGTTGAA TTTCAGCATC CTTATTTCAA GCAAGGCCAG 
GATGACCTGT TGGAGAACAT TAAAAGGAAG GTTTCATCTT CAAAACCAGA GGAAAATAAA 
ATTCGTCAGG AAGATTTAAC AAAAATTATT AGTAGTGCTC AGAAGGTTCA AATAAAACAA 
GAAACTATTG AGTCCAGGCT TTCAGAATTA AAAAGTGAGA ATGAATCCCT TTGGAAGGAG 
GTGTCAGAAC TAAGAGCAAA GCATGCCCAG CAGCAACAAG TTATTCGGAA GATTGTCCAG 
TTTATTGTTA CATTGGTTCA GAATAATCAA CTTGTGAGTT TAAAACGTAA AAGGCCTCTA 
CTTCTAAACA CAAATGGAGC CCCAAAGAAG AATCTATATC AGCACATAGT CAAAGAACCA 
ACTGATAATC ACCATCATAA AGTTCCACAC AGCAGGACTG AAGGTTTAAA GTCAAGAGAA 
CGGATTTCAG ATGACATAAT TATTTATGAT GTTACTGACG ATAATGTGGA TGAAGAAAAT 
ATTCCAGTTA TTCCAGAAAC AAATGAGGAT GTTGTAGTGG ATTCCTCCAA CCAGTATCCT 
GACATTGTCA TTGTTGAAGA TGACAACGAG GATGAGTATG CTCCTGTCAT TCAGAGTGGA 
GAGCAGAGTG AACCAGCCAG AGAACCCTTA CGTGTGGGGA GTGCTGGCAG CAGCAGCCCT 
CTCATGTCTA GTGCTGTCCA GCTAAACGGC TCCTCCAGTC TGACCTCAGA AGACCCTGTG 
ACCATGATGG ACTCCATTCT GAATGACAAC ATTAACCTGT TAGGAAAGGT TGAGCTGTTG 
GATTACCTTG ACAGTATTGA TTGCAGTTTA GAGGACTTCC AAGCTATGCT CTCAGGAAGA 
CAGTTTAGCA TAGACCCAGA TCTTCTGGTT GATTCTGAGA ATAAGGGACT AGAAGCTACC 
AAGAGCAGTG TTGTTCAACA TGTGTCAGAA GAGGGAAGAA AATCTAAATC CAAGCCAGAC 
AAACAACTTA TCCAGTATAC TGCCTTTCCA CTTCTTGCAT TCCTGGATGG GAACTCTGCA 
TCTGCTATTG AACAGGGGAG TACAACTGCA TCGTCAGAAG TTGTGCCTTC TGTAGATAAA 
CCCATAGAAG TCGATGAGCT CCTGGATAGC AGCCTGGATC CAGAACCGAC CCAGAGTAAG 
CTTGTCCGCC TGGAACCATT GACTGAAGCG GAAGCTAGTG AAGCCACACT CTTCTATTTA 
TGTGAACTTG CTCCTGCACC TCTGGATAGT GATATGCCGC TTTTAGATAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032323.3
CDS	125..1678
Translation	MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

Target ORF information:

RefSeq Version	NM_008297.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock factor 2 (Hsf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008297.3

ORF Insert Sequence:

ATGAAGCAGA GTTCCAACGT GCCGGCTTTC CTCAGCAAGC TGTGGACGCT TGTGGAGGAA 
ACCCACACCA ACGAGTTCAT CACCTGGAGT CAGAATGGAC AAAGTTTTCT GGTCTTGGAT 
GAGCAAAGAT TTGCAAAGGA AATTCTTCCT AAGTACTTCA AACACAATAA CATGGCGAGC 
TTTGTGAGAC AACTAAATAT GTATGGCTTC CGAAAAGTAG TGCATATCGA ATCTGGAATT 
ATCAAACAGG AAAGAGATGG CCCTGTTGAA TTTCAGCATC CTTATTTCAA GCAAGGCCAG 
GATGACCTGT TGGAGAACAT TAAAAGGAAG GTTTCATCTT CAAAACCAGA GGAAAATAAA 
ATTCGTCAGG AAGATTTAAC AAAAATTATT AGTAGTGCTC AGAAGGTTCA AATAAAACAA 
GAAACTATTG AGTCCAGGCT TTCAGAATTA AAAAGTGAGA ATGAATCCCT TTGGAAGGAG 
GTGTCAGAAC TAAGAGCAAA GCATGCCCAG CAGCAACAAG TTATTCGGAA GATTGTCCAG 
TTTATTGTTA CATTGGTTCA GAATAATCAA CTTGTGAGTT TAAAACGTAA AAGGCCTCTA 
CTTCTAAACA CAAATGGAGC CCCAAAGAAG AATCTATATC AGCACATAGT CAAAGAACCA 
ACTGATAATC ACCATCATAA AGTTCCACAC AGCAGGACTG AAGGTTTAAA GTCAAGAGAA 
CGGATTTCAG ATGACATAAT TATTTATGAT GTTACTGACG ATAATGTGGA TGAAGAAAAT 
ATTCCAGTTA TTCCAGAAAC AAATGAGGAT GTTGTAGTGG ATTCCTCCAA CCAGTATCCT 
GACATTGTCA TTGTTGAAGA TGACAACGAG GATGAGTATG CTCCTGTCAT TCAGAGTGGA 
GAGCAGAGTG AACCAGCCAG AGAACCCTTA CGTGTGGGGA GTGCTGGCAG CAGCAGCCCT 
CTCATGTCTA GTGCTGTCCA GCTAAACGGC TCCTCCAGTC TGACCTCAGA AGACCCTGTG 
ACCATGATGG ACTCCATTCT GAATGACAAC ATTAACCTGT TAGGAAAGGT TGAGCTGTTG 
GATTACCTTG ACAGTATTGA TTGCAGTTTA GAGGACTTCC AAGCTATGCT CTCAGGAAGA 
CAGTTTAGCA TAGACCCAGA TCTTCTGGTT GATTCTGAGA ATAAGGGACT AGAAGCTACC 
AAGAGCAGTG TTGTTCAACA TGTGTCAGAA GAGGGAAGAA AATCTAAATC CAAGCCAGAC 
AAACAACTTA TCCAGTATAC TGCCTTTCCA CTTCTTGCAT TCCTGGATGG GAACTCTGCA 
TCTGCTATTG AACAGGGGAG TACAACTGCA TCGTCAGAAG TTGTGCCTTC TGTAGATAAA 
CCCATAGAAG TCGATGAGCT CCTGGATAGC AGCCTGGATC CAGAACCGAC CCAGAGTAAG 
CTTGTCCGCC TGGAACCATT GACTGAAGCG GAAGCTAGTG AAGCCACACT CTTCTATTTA 
TGTGAACTTG CTCCTGCACC TCTGGATAGT GATATGCCGC TTTTAGATAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsf2 ( XM_006512564.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512627.1 Mus musculus heat shock factor 2 (Hsf2), transcript variant X1, mRNA.

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CloneID	OMu43736
Clone ID Related Accession (Same CDS sequence)	XM_006512564.2 , XM_006512564.3
Accession Version	XM_006512564.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	heat shock factor protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGA GTTCCAACGT GCCGGCTTTC CTCAGCAAGC TGTGGACGCT TGTGGAGGAA 
ACCCACACCA ACGAGTTCAT CACCTGGAGT CAGAATGGAC AAAGTTTTCT GGTCTTGGAT 
GAGCAAAGAT TTGCAAAGGA AATTCTTCCT AAGTACTTCA AACACAATAA CATGGCGAGC 
TTTGTGAGAC AACTAAATAT GTATGGCTTC CGAAAAGTAG TGCATATCGA ATCTGGAATT 
ATCAAACAGG AAAGAGATGG CCCTGTTGAA TTTCAGCATC CTTATTTCAA GCAAGGCCAG 
GATGACCTGT TGGAGAACAT TAAAAGGAAG GTTTCATCTT CAAAACCAGA GGAAAATAAA 
ATTCGTCAGG AAGATTTAAC AAAAATTATT AGTAGTGCTC AGAAGGTTCA AATAAAACAA 
GAAACTATTG AGTCCAGGCT TTCAGAATTA AAAAGTGAGA ATGAATCCCT TTGGAAGGAG 
GTGTCAGAAC TAAGAGCAAA GCATGCCCAG CAGCAACAAG TTATTCGGAA GATTGTCCAG 
TTTATTGTTA CATTGGTTCA GAATAATCAA CTTGTGAGTT TAAAACGTAA AAGGCCTCTA 
CTTCTAAACA CAAATGGAGC CCCAAAGAAG AATCTATATC AGCACATAGT CAAAGAACCA 
ACTGATAATC ACCATCATAA AGTTCCACAC AGCAGGACTG AAGGTTTAAA GTCAAGAGAA 
CGGATTTCAG ATGACATAAT TATTTATGAT GTTACTGACG ATAATGTGGA TGAAGAAAAT 
ATTCCAGTTA TTCCAGAAAC AAATGAGGAT GTTGTAGTGG ATTCCTCCAA CCAGTATCCT 
GACATTGTCA TTGTTGAAGA TGACAACGAG GATGAGTATG CTCCTGTCAT TCAGAGTGGA 
GAGCAGAGTG AACCAGCCAG AGAACCCTTA CGTGTGGGGA GTGCTGGCAG CAGCAGCCCT 
CTCATGTCTA GTGCTGTCCA GCTAAACGGC TCCTCCAGTC TGACCTCAGA AGACCCTGTG 
ACCATGATGG ACTCCATTCT GAATGACAAC ATTAACCTGT TAGGAAAGGT TGAGCTGTTG 
GATTACCTTG ACAGTATTGA TTGCAGTTTA GAGGACTTCC AAGCTATGCT CTCAGGAAGA 
CAGTTTAGCA TAGACCCAGA TCTTCTGGTT GATCTTTTCA CTAGTTCTGT GCAGATGAAT 
CCCACAGATA ACATCAATAA TACAAAATCT GAGAATAAGG GACTAGAAGC TACCAAGAGC 
AGTGTTGTTC AACATGTGTC AGAAGAGGGA AGAAAATCTA AATCCAAGCC AGACAAACAA 
CTTATCCAGT ATACTGCCTT TCCACTTCTT GCATTCCTGG ATGGGAACTC TGCATCTGCT 
ATTGAACAGG GGAGTACAAC TGCATCGTCA GAAGTTGTGC CTTCTGTAGA TAAACCCATA 
GAAGTCGATG AGCTCCTGGA TAGCAGCCTG GATCCAGAAC CGACCCAGAG TAAGCTTGTC 
CGCCTGGAAC CATTGACTGA AGCGGAAGCT AGTGAAGCCA CACTCTTCTA TTTATGTGAA 
CTTGCTCCTG CACCTCTGGA TAGTGATATG CCGCTTTTAG ATAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512627.1
CDS	2744..4351
Translation	MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIESGIIKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAPKKNLYQHIVKEPTDNHHHKVPHSRTEGLKSRERISDDIIIYDVTDDNVDEENIPVIPETNEDVVVDSSNQYPDIVIVEDDNEDEYAPVIQSGEQSEPAREPLRVGSAGSSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDNINNTKSENKGLEATKSSVVQHVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNSASAIEQGSTTASSEVVPSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

Target ORF information:

RefSeq Version	XM_006512564.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus heat shock factor 2 (Hsf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512564.3

ORF Insert Sequence:

ATGAAGCAGA GTTCCAACGT GCCGGCTTTC CTCAGCAAGC TGTGGACGCT TGTGGAGGAA 
ACCCACACCA ACGAGTTCAT CACCTGGAGT CAGAATGGAC AAAGTTTTCT GGTCTTGGAT 
GAGCAAAGAT TTGCAAAGGA AATTCTTCCT AAGTACTTCA AACACAATAA CATGGCGAGC 
TTTGTGAGAC AACTAAATAT GTATGGCTTC CGAAAAGTAG TGCATATCGA ATCTGGAATT 
ATCAAACAGG AAAGAGATGG CCCTGTTGAA TTTCAGCATC CTTATTTCAA GCAAGGCCAG 
GATGACCTGT TGGAGAACAT TAAAAGGAAG GTTTCATCTT CAAAACCAGA GGAAAATAAA 
ATTCGTCAGG AAGATTTAAC AAAAATTATT AGTAGTGCTC AGAAGGTTCA AATAAAACAA 
GAAACTATTG AGTCCAGGCT TTCAGAATTA AAAAGTGAGA ATGAATCCCT TTGGAAGGAG 
GTGTCAGAAC TAAGAGCAAA GCATGCCCAG CAGCAACAAG TTATTCGGAA GATTGTCCAG 
TTTATTGTTA CATTGGTTCA GAATAATCAA CTTGTGAGTT TAAAACGTAA AAGGCCTCTA 
CTTCTAAACA CAAATGGAGC CCCAAAGAAG AATCTATATC AGCACATAGT CAAAGAACCA 
ACTGATAATC ACCATCATAA AGTTCCACAC AGCAGGACTG AAGGTTTAAA GTCAAGAGAA 
CGGATTTCAG ATGACATAAT TATTTATGAT GTTACTGACG ATAATGTGGA TGAAGAAAAT 
ATTCCAGTTA TTCCAGAAAC AAATGAGGAT GTTGTAGTGG ATTCCTCCAA CCAGTATCCT 
GACATTGTCA TTGTTGAAGA TGACAACGAG GATGAGTATG CTCCTGTCAT TCAGAGTGGA 
GAGCAGAGTG AACCAGCCAG AGAACCCTTA CGTGTGGGGA GTGCTGGCAG CAGCAGCCCT 
CTCATGTCTA GTGCTGTCCA GCTAAACGGC TCCTCCAGTC TGACCTCAGA AGACCCTGTG 
ACCATGATGG ACTCCATTCT GAATGACAAC ATTAACCTGT TAGGAAAGGT TGAGCTGTTG 
GATTACCTTG ACAGTATTGA TTGCAGTTTA GAGGACTTCC AAGCTATGCT CTCAGGAAGA 
CAGTTTAGCA TAGACCCAGA TCTTCTGGTT GATCTTTTCA CTAGTTCTGT GCAGATGAAT 
CCCACAGATA ACATCAATAA TACAAAATCT GAGAATAAGG GACTAGAAGC TACCAAGAGC 
AGTGTTGTTC AACATGTGTC AGAAGAGGGA AGAAAATCTA AATCCAAGCC AGACAAACAA 
CTTATCCAGT ATACTGCCTT TCCACTTCTT GCATTCCTGG ATGGGAACTC TGCATCTGCT 
ATTGAACAGG GGAGTACAAC TGCATCGTCA GAAGTTGTGC CTTCTGTAGA TAAACCCATA 
GAAGTCGATG AGCTCCTGGA TAGCAGCCTG GATCCAGAAC CGACCCAGAG TAAGCTTGTC 
CGCCTGGAAC CATTGACTGA AGCGGAAGCT AGTGAAGCCA CACTCTTCTA TTTATGTGAA 
CTTGCTCCTG CACCTCTGGA TAGTGATATG CCGCTTTTAG ATAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(9)3155-70(2016 09)
Borowiec AS,Sion B,Chalmel F,D Rolland A,Lemonnier L,De Clerck T,Bokhobza A,Derouiche S,Dewailly E,Slomianny C,Mauduit C,Benahmed M,Roudbaraki M,J?gou B,Prevarskaya N,Bidaux G

Set1/MLL complex is indispensable for the transcriptional ability of heat shock transcription factor 2.
Biochemical and biophysical research communications467(4)805-12(2015 Nov)
Hayashida N

HSF2 autoregulates its own transcription.
International journal of molecular medicine36(4)1173-9(2015 Oct)
Park SM,Kim SA,Ahn SG

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Hsf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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