Hmbs cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmbs
Entrez Gene ID	15288
Full Name	hydroxymethylbilane synthase
Synonyms	PBGD,T25658,Ups,Uros1
General protein information	Preferred Names hydroxymethylbilane synthase Names porphobilinogen deaminase PBG-D pre-uroporphyrinogen synthase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A5.2\|9 24.84 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013551.2	NP_038579.2	porphobilinogen deaminase isoform 1
NM_001110251.1	NP_001103721.1	porphobilinogen deaminase isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hmbs	NP_038579.2
Gallus gallus (chicken)	HMBS	XP_417846.2
Saccharomyces cerevisiae (baker's yeast)	HEM3	NP_010076.1
Schizosaccharomyces pombe (fission yeast)	hem3	NP_001018179.1
Arabidopsis thaliana (thale cress)	HEMC	NP_196445.1
Canis lupus familiaris (dog)	HMBS	XP_546491.2
Oryza sativa (rice)	Os02g0168800	NP_001046017.1
	HMBS	XP_001101764.1
Bos taurus (cattle)	HMBS	NP_001039672.1
Rattus norvegicus (Norway rat)	Hmbs	NP_037300.2
Danio rerio (zebrafish)	hmbsa	NP_957448.1
Drosophila melanogaster (fruit fly)	l(3)02640	NP_612103.1
Neurospora crassa	NCU10292	XP_001728039.1
Homo sapiens (human)	HMBS	NP_000181.2
Danio rerio (zebrafish)	hmbsb	NP_001019559.1
Kluyveromyces lactis	KLLA0C15147g	XP_452879.1
Magnaporthe oryzae (rice blast fungus)	MGG_04860	XP_003712340.1
Xenopus tropicalis (tropical clawed frog)	hmbs	NP_001005635.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011080	XP_564464.3
Eremothecium gossypii	AGOS_ABL107C	NP_982840.1

Gene Ontology
Bibliography

Related articles in PubMed

Glucose metabolism during fasting is altered in experimental porphobilinogen deaminase deficiency.
Collantes M, Serrano-Mendioroz I, Benito M, Molinet-Dronda F, Delgado M, Vinaixa M, Sampedro A, Enr?quez de Salamanca R, Prieto E, Pozo MA, Pe?uelas I, Corrales FJ, Barajas M, Fontanellas A
Human molecular genetics25(7)1318-27(2016 Apr)

Mitochondrial energetic defects in muscle and brain of a Hmbs-/- mouse model of acute intermittent porphyria.
Homedan C, Schmitt C, Laafi J, Gueguen N, Desquiret-Dumas V, Lenglet H, Karim Z, Gouya L, Deybach JC, Simard G, Puy H, Malthi?ry Y, Reynier P
Human molecular genetics24(17)5015-23(2015 Sep)

The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice.
Jakobs HH, Mikula M, Havemeyer A, Strzalkowska A, Borowa-Chmielak M, Dzwonek A, Gajewska M, Hennig EE, Ostrowski J, Clement B
PloS one9(8)e105371(2014)

Helper-dependent adenoviral liver gene therapy protects against induced attacks and corrects protein folding stress in acute intermittent porphyria mice.
Unzu C, Sampedro A, Maule?n I, Gonz?lez-Aparicio M, Enr?quez de Salamanca R, Prieto J, Arag?n T, Fontanellas A
Human molecular genetics22(14)2929-40(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PBGD-deficient mice showed a different response to fasting as measured by altered carbohydrate metabolism in the liver and modified glucose consumption in the brain cortex. Glucose homeostasis in fasted PBGD-deficient mice was efficiently normalized after restoration of PBGD gene expression in the liver.
Title: Glucose metabolism during fasting is altered in experimental porphobilinogen deaminase deficiency.

Porphobilinogen deaminase over-expression in hepatocytes, albeit in a low proportion, reduced precursor accumulation, which is the hallmark of acute porphyric attacks.
Title: Porphobilinogen deaminase over-expression in hepatocytes, but not in erythrocytes, prevents accumulation of toxic porphyrin precursors in a mouse model of acute intermittent porphyria.

PBGD-deficient mice were characterized biochemically during phenobarbital induction of heme synthesis and enzyme replacement.
Title: Biochemical characterization of porphobilinogen deaminase-deficient mice during phenobarbital induction of heme synthesis and the effect of enzyme replacement.

The following Hmbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18816	NM_001110251.1 Latest version!	Mus musculus hydroxymethylbilane synthase (Hmbs), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu18872	NM_013551.2 Latest version!	Mus musculus hydroxymethylbilane synthase (Hmbs), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmbs ( NM_001110251.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103721.1 Mus musculus hydroxymethylbilane synthase (Hmbs), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18816
Clone ID Related Accession (Same CDS sequence)	NM_001110251.1
Accession Version	NM_001110251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	porphobilinogen deaminase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB844509.1, AK143558.1 and AI853649.1. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143558.1, AK028234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGTGA TTCGAGTGGG CACCCGTAAG AGCCAGCTGG CTCGCATACA GACCGACACT 
GTGGTGGCGA TGCTGAAAGC CTTGTACCCT GGCATACAGT TTGAAATCAT TGCTATGTCC 
ACCACGGGAG ACAAGATTCT TGATACTGCA CTCTCTAAGA TTGGAGAGAA GAGCCTGTTT 
ACCAAGGAGC TAGAAAACGC CCTGGAAAAA AACGAAGTGG ACCTGGTCGT TCACTCCCTG 
AAGGATGTGC CTACCATACT ACCTCCTGGC TTTACTATTG GAGCCATCTG CAAACGGGAA 
AACCCTTGTG ATGCTGTTGT CTTTCACCCA AAGTTTATTG GAAAGACCCT GGAAACCTTG 
CCAGAGAAAA GTGCCGTGGG AACCAGCTCT CTGAGGAGAG TGGCTCAGCT ACAGAGAAAG 
TTCCCCCACC TGGAATTCAA GAGTATTCGG GGAAACCTCA ACACCCGCCT CCGGAAGCTG 
GATGAGCTGC AGGAATTCAG TGCCATCGTC CTGGCTGTGG CTGGCCTACA GCGCATGGGC 
TGGCAGAACC GGGTGGGCCA GATTTTGCAC CCAGAAGAAT GCATGTATGC TGTGGGTCAG 
GGAGCCCTAG CCGTGGAAGT CCGAGCCAAG GACCAGGATA TCTTGGACCT AGTGAGTGTG 
TTGCACGATC CTGAAACTCT GCTTCGCTGC ATTGCTGAAA GGGCTTTTCT GAGGCACCTG 
GAAGGAGGCT GCAGCGTGCC CGTAGCAGTG CATACAGTGA TGAAAGATGG GCAACTGTAC 
CTGACTGGTG GAGTCTGGAG TCTAGATGGC TCAGATAGCA TGCAAGAGAC TATGCAGGCC 
ACCATCCAGG TCCCTGTTCA GCAAGAAGAT GGTCCAGAAG ATGACCCACA ACTGGTTGGA 
ATCACTGCCC GTAACATTCC AAGAGGAGCC CAGCTAGCTG CTGAGAACCT GGGCATCAGC 
CTGGCCAGCT TGCTGCTCAA CAAAGGAGCC AAGAACATCC TGGATGTTGC ACGGCAGCTT 
AATGATGTGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103721.1
CDS	180..1214
Translation	MRVIRVGTRKSQLARIQTDTVVAMLKALYPGIQFEIIAMSTTGDKILDTALSKIGEKSLFTKELENALEKNEVDLVVHSLKDVPTILPPGFTIGAICKRENPCDAVVFHPKFIGKTLETLPEKSAVGTSSLRRVAQLQRKFPHLEFKSIRGNLNTRLRKLDELQEFSAIVLAVAGLQRMGWQNRVGQILHPEECMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTGGVWSLDGSDSMQETMQATIQVPVQQEDGPEDDPQLVGITARNIPRGAQLAAENLGISLASLLLNKGAKNILDVARQLNDVR

Target ORF information:

RefSeq Version	NM_001110251.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxymethylbilane synthase (Hmbs), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110251.1

ORF Insert Sequence:

ATGAGGGTGA TTCGAGTGGG CACCCGTAAG AGCCAGCTGG CTCGCATACA GACCGACACT 
GTGGTGGCGA TGCTGAAAGC CTTGTACCCT GGCATACAGT TTGAAATCAT TGCTATGTCC 
ACCACGGGAG ACAAGATTCT TGATACTGCA CTCTCTAAGA TTGGAGAGAA GAGCCTGTTT 
ACCAAGGAGC TAGAAAACGC CCTGGAAAAA AACGAAGTGG ACCTGGTCGT TCACTCCCTG 
AAGGATGTGC CTACCATACT ACCTCCTGGC TTTACTATTG GAGCCATCTG CAAACGGGAA 
AACCCTTGTG ATGCTGTTGT CTTTCACCCA AAGTTTATTG GAAAGACCCT GGAAACCTTG 
CCAGAGAAAA GTGCCGTGGG AACCAGCTCT CTGAGGAGAG TGGCTCAGCT ACAGAGAAAG 
TTCCCCCACC TGGAATTCAA GAGTATTCGG GGAAACCTCA ACACCCGCCT CCGGAAGCTG 
GATGAGCTGC AGGAATTCAG TGCCATCGTC CTGGCTGTGG CTGGCCTACA GCGCATGGGC 
TGGCAGAACC GGGTGGGCCA GATTTTGCAC CCAGAAGAAT GCATGTATGC TGTGGGTCAG 
GGAGCCCTAG CCGTGGAAGT CCGAGCCAAG GACCAGGATA TCTTGGACCT AGTGAGTGTG 
TTGCACGATC CTGAAACTCT GCTTCGCTGC ATTGCTGAAA GGGCTTTTCT GAGGCACCTG 
GAAGGAGGCT GCAGCGTGCC CGTAGCAGTG CATACAGTGA TGAAAGATGG GCAACTGTAC 
CTGACTGGTG GAGTCTGGAG TCTAGATGGC TCAGATAGCA TGCAAGAGAC TATGCAGGCC 
ACCATCCAGG TCCCTGTTCA GCAAGAAGAT GGTCCAGAAG ATGACCCACA ACTGGTTGGA 
ATCACTGCCC GTAACATTCC AAGAGGAGCC CAGCTAGCTG CTGAGAACCT GGGCATCAGC 
CTGGCCAGCT TGCTGCTCAA CAAAGGAGCC AAGAACATCC TGGATGTTGC ACGGCAGCTT 
AATGATGTGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmbs ( NM_013551.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038579.2 Mus musculus hydroxymethylbilane synthase (Hmbs), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18872
Clone ID Related Accession (Same CDS sequence)	NM_013551.2
Accession Version	NM_013551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	porphobilinogen deaminase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY786671.1, AK151020.1, AK166734.1 and AI853649.1. On Nov 2, 2007 this sequence version replaced NM_013551.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003861.1, AK167702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGTA ACGGCGGCGC GGCCACAACC GCGGAAGAAA ACGGCTCAAA GATGAGGGTG 
ATTCGAGTGG GCACCCGTAA GAGCCAGCTG GCTCGCATAC AGACCGACAC TGTGGTGGCG 
ATGCTGAAAG CCTTGTACCC TGGCATACAG TTTGAAATCA TTGCTATGTC CACCACGGGA 
GACAAGATTC TTGATACTGC ACTCTCTAAG ATTGGAGAGA AGAGCCTGTT TACCAAGGAG 
CTAGAAAACG CCCTGGAAAA AAACGAAGTG GACCTGGTCG TTCACTCCCT GAAGGATGTG 
CCTACCATAC TACCTCCTGG CTTTACTATT GGAGCCATCT GCAAACGGGA AAACCCTTGT 
GATGCTGTTG TCTTTCACCC AAAGTTTATT GGAAAGACCC TGGAAACCTT GCCAGAGAAA 
AGTGCCGTGG GAACCAGCTC TCTGAGGAGA GTGGCTCAGC TACAGAGAAA GTTCCCCCAC 
CTGGAATTCA AGAGTATTCG GGGAAACCTC AACACCCGCC TCCGGAAGCT GGATGAGCTG 
CAGGAATTCA GTGCCATCGT CCTGGCTGTG GCTGGCCTAC AGCGCATGGG CTGGCAGAAC 
CGGGTGGGCC AGATTTTGCA CCCAGAAGAA TGCATGTATG CTGTGGGTCA GGGAGCCCTA 
GCCGTGGAAG TCCGAGCCAA GGACCAGGAT ATCTTGGACC TAGTGAGTGT GTTGCACGAT 
CCTGAAACTC TGCTTCGCTG CATTGCTGAA AGGGCTTTTC TGAGGCACCT GGAAGGAGGC 
TGCAGCGTGC CCGTAGCAGT GCATACAGTG ATGAAAGATG GGCAACTGTA CCTGACTGGT 
GGAGTCTGGA GTCTAGATGG CTCAGATAGC ATGCAAGAGA CTATGCAGGC CACCATCCAG 
GTCCCTGTTC AGCAAGAAGA TGGTCCAGAA GATGACCCAC AACTGGTTGG AATCACTGCC 
CGTAACATTC CAAGAGGAGC CCAGCTAGCT GCTGAGAACC TGGGCATCAG CCTGGCCAGC 
TTGCTGCTCA ACAAAGGAGC CAAGAACATC CTGGATGTTG CACGGCAGCT TAATGATGTG 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038579.2
CDS	187..1272
Translation	MSGNGGAATTAEENGSKMRVIRVGTRKSQLARIQTDTVVAMLKALYPGIQFEIIAMSTTGDKILDTALSKIGEKSLFTKELENALEKNEVDLVVHSLKDVPTILPPGFTIGAICKRENPCDAVVFHPKFIGKTLETLPEKSAVGTSSLRRVAQLQRKFPHLEFKSIRGNLNTRLRKLDELQEFSAIVLAVAGLQRMGWQNRVGQILHPEECMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTGGVWSLDGSDSMQETMQATIQVPVQQEDGPEDDPQLVGITARNIPRGAQLAAENLGISLASLLLNKGAKNILDVARQLNDVR

Target ORF information:

RefSeq Version	NM_013551.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxymethylbilane synthase (Hmbs), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013551.2

ORF Insert Sequence:

ATGTCCGGTA ACGGCGGCGC GGCCACAACC GCGGAAGAAA ACGGCTCAAA GATGAGGGTG 
ATTCGAGTGG GCACCCGTAA GAGCCAGCTG GCTCGCATAC AGACCGACAC TGTGGTGGCG 
ATGCTGAAAG CCTTGTACCC TGGCATACAG TTTGAAATCA TTGCTATGTC CACCACGGGA 
GACAAGATTC TTGATACTGC ACTCTCTAAG ATTGGAGAGA AGAGCCTGTT TACCAAGGAG 
CTAGAAAACG CCCTGGAAAA AAACGAAGTG GACCTGGTCG TTCACTCCCT GAAGGATGTG 
CCTACCATAC TACCTCCTGG CTTTACTATT GGAGCCATCT GCAAACGGGA AAACCCTTGT 
GATGCTGTTG TCTTTCACCC AAAGTTTATT GGAAAGACCC TGGAAACCTT GCCAGAGAAA 
AGTGCCGTGG GAACCAGCTC TCTGAGGAGA GTGGCTCAGC TACAGAGAAA GTTCCCCCAC 
CTGGAATTCA AGAGTATTCG GGGAAACCTC AACACCCGCC TCCGGAAGCT GGATGAGCTG 
CAGGAATTCA GTGCCATCGT CCTGGCTGTG GCTGGCCTAC AGCGCATGGG CTGGCAGAAC 
CGGGTGGGCC AGATTTTGCA CCCAGAAGAA TGCATGTATG CTGTGGGTCA GGGAGCCCTA 
GCCGTGGAAG TCCGAGCCAA GGACCAGGAT ATCTTGGACC TAGTGAGTGT GTTGCACGAT 
CCTGAAACTC TGCTTCGCTG CATTGCTGAA AGGGCTTTTC TGAGGCACCT GGAAGGAGGC 
TGCAGCGTGC CCGTAGCAGT GCATACAGTG ATGAAAGATG GGCAACTGTA CCTGACTGGT 
GGAGTCTGGA GTCTAGATGG CTCAGATAGC ATGCAAGAGA CTATGCAGGC CACCATCCAG 
GTCCCTGTTC AGCAAGAAGA TGGTCCAGAA GATGACCCAC AACTGGTTGG AATCACTGCC 
CGTAACATTC CAAGAGGAGC CCAGCTAGCT GCTGAGAACC TGGGCATCAG CCTGGCCAGC 
TTGCTGCTCA ACAAAGGAGC CAAGAACATC CTGGATGTTG CACGGCAGCT TAATGATGTG 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucose metabolism during fasting is altered in experimental porphobilinogen deaminase deficiency.
Human molecular genetics25(7)1318-27(2016 Apr)
Collantes M,Serrano-Mendioroz I,Benito M,Molinet-Dronda F,Delgado M,Vinaixa M,Sampedro A,Enr?quez de Salamanca R,Prieto E,Pozo MA,Pe?uelas I,Corrales FJ,Barajas M,Fontanellas A

Mitochondrial energetic defects in muscle and brain of a Hmbs-/- mouse model of acute intermittent porphyria.
Human molecular genetics24(17)5015-23(2015 Sep)
Homedan C,Schmitt C,Laafi J,Gueguen N,Desquiret-Dumas V,Lenglet H,Karim Z,Gouya L,Deybach JC,Simard G,Puy H,Malthi?ry Y,Reynier P

The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice.
PloS one9(8)e105371(2014)
Jakobs HH,Mikula M,Havemeyer A,Strzalkowska A,Borowa-Chmielak M,Dzwonek A,Gajewska M,Hennig EE,Ostrowski J,Clement B

Helper-dependent adenoviral liver gene therapy protects against induced attacks and corrects protein folding stress in acute intermittent porphyria mice.
Human molecular genetics22(14)2929-40(2013 Jul)
Unzu C,Sampedro A,Maule?n I,Gonz?lez-Aparicio M,Enr?quez de Salamanca R,Prieto J,Arag?n T,Fontanellas A