Hif1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hif1a
Entrez Gene ID	15251
Full Name	hypoxia inducible factor 1, alpha subunit
Synonyms	AA959795,HIF-1-alpha,HIF1-alpha,HIF1alpha,MOP1,bHLHe78
General protein information	Preferred Names hypoxia inducible factor 1, alpha subunit Names hypoxia-inducible factor 1-alpha ARNT-interacting protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 C3\|12 31.99 cM
Summary	This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515478.4	XP_006515541.1	hypoxia-inducible factor 1-alpha isoform X1
NM_001313919.1	NP_001300848.1	hypoxia-inducible factor 1-alpha isoform 1
XM_017314961.1	XP_017170450.1	hypoxia-inducible factor 1-alpha isoform X2
NM_001313920.1	NP_001300849.1	hypoxia-inducible factor 1-alpha isoform 3
NM_010431.2	NP_034561.2	hypoxia-inducible factor 1-alpha isoform 2
XM_006515478.3	XP_006515541.1	hypoxia-inducible factor 1-alpha isoform X1
XM_017314961.2	XP_017170450.1	hypoxia-inducible factor 1-alpha isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HIF1A	XP_003639249.1
Homo sapiens (human)	HIF1A	NP_001521.1
Rattus norvegicus (Norway rat)	Hif1a	NP_077335.1
	HIF1A	XP_001099149.1
Bos taurus (cattle)	HIF1A	NP_776764.2
Mus musculus (house mouse)	Hif1a	NP_034561.2
Danio rerio (zebrafish)	hif1ab	NP_956527.1
Pan troglodytes (chimpanzee)	HIF1A	XP_001168972.1
Gallus gallus (chicken)	HIF1A	NP_989628.1

Gene Ontology

The following Hif1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hif1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67950	NM_001313920.1 Latest version!	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu22900	NM_010431.2 Latest version!	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu47586	NM_001313919.1 Latest version!	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu47587	XM_006515478.4 Latest version!	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu78147	XM_017314961.2 Latest version!	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu47587	XM_006515478.3	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu78147	XM_017314961.1	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hif1a ( NM_001313920.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300849.1 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 3, mRNA.

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CloneID	OMu67950
Clone ID Related Accession (Same CDS sequence)	NM_001313920.1
Accession Version	NM_001313920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124712.5. On Sep 11, 2015 this sequence version replaced XM_011243997.1. Transcript Variant: This variant (3) has an alternate 5' structure, which results in the use of a downstream start codon, and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X95580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTG AACGTCGAAA AGAAAAGTCT AGAGATGCAG CAAGATCTCG GCGAAGCAAA 
GAGTCTGAAG TTTTTTATGA GCTTGCTCAT CAGTTGCCAC TTCCCCACAA TGTGAGCTCA 
CATCTTGATA AAGCTTCTGT TATGAGGCTC ACCATCAGTT ATTTACGTGT GAGAAAACTT 
CTGGATGCCG GTGGTCTAGA CAGTGAAGAT GAGATGAAGG CACAGATGGA CTGTTTTTAT 
CTGAAAGCCC TAGATGGCTT TGTGATGGTG CTAACAGATG ACGGCGACAT GGTTTACATT 
TCTGATAACG TGAACAAATA CATGGGGTTA ACTCAGTTTG AACTAACTGG ACACAGTGTG 
TTTGATTTTA CTCATCCATG TGACCATGAG GAAATGAGAG AAATGCTTAC ACACAGAAAT 
GGCCCAGTGA GAAAAGGGAA AGAACTAAAC ACACAGCGGA GCTTTTTTCT CAGAATGAAG 
TGCACCCTAA CAAGCCGGGG GAGGACGATG AACATCAAGT CAGCAACGTG GAAGGTGCTT 
CACTGCACGG GCCATATTCA TGTCTATGAT ACCAACAGTA ACCAACCTCA GTGTGGGTAC 
AAGAAACCAC CCATGACGTG CTTGGTGCTG ATTTGTGAAC CCATTCCTCA TCCGTCAAAT 
ATTGAAATTC CTTTAGATAG CAAGACATTT CTCAGTCGAC ACAGCCTCGA TATGAAATTT 
TCTTACTGTG ATGAAAGAAT TACTGAGTTG ATGGGTTATG AGCCGGAAGA ACTTTTGGGC 
CGCTCAATTT ATGAATATTA TCATGCTTTG GATTCTGATC ATCTGACCAA AACTCACCAT 
GATATGTTTA CTAAAGGACA AGTCACCACA GGACAGTACA GGATGCTTGC CAAAAGAGGT 
GGATATGTCT GGGTTGAAAC TCAAGCAACT GTCATATATA ATACGAAGAA CTCCCAGCCA 
CAGTGCATTG TGTGTGTGAA TTATGTTGTA AGTGGTATTA TTCAGCACGA CTTGATTTTC 
TCCCTTCAAC AAACAGAATC TGTGCTCAAA CCAGTTGAAT CTTCAGATAT GAAGATGACT 
CAGCTGTTCA CCAAAGTTGA ATCAGAGGAT ACAAGCTGCC TTTTTGATAA GCTTAAGAAG 
GAGCCTGATG CTCTCACTCT GCTGGCTCCA GCTGCCGGCG ACACCATCAT CTCTCTGGAT 
TTTGGCAGCG ATGACACAGA AACTGAAGAT CAACAACTTG AAGATGTTCC ATTATATAAT 
GATGTAATGT TTCCCTCTTC TAATGAAAAA TTAAATATAA ACCTGGCAAT GTCTCCTTTA 
CCTTCATCGG AAACTCCAAA GCCACTTCGA AGTAGTGCTG ATCCTGCACT GAATCAAGAG 
GTTGCATTAA AATTAGAATC AAGTCCAGAG TCACTGGGAC TTTCTTTTAC CATGCCCCAG 
ATTCAAGATC AGCCAGCAAG TCCTTCTGAT GGAAGCACTA GACAAAGTTC ACCTGAGCCT 
AACAGTCCCA GTGAATATTG CTTTGATGTG GATAGCGATA TGGTCAATGT ATTCAAGTTG 
GAACTGGTGG AAAAACTGTT TGCTGAAGAC ACAGAGGCAA AGAATCCATT TTCAACTCAG 
GACACTGATT TAGATTTGGA GATGCTGGCT CCCTATATCC CAATGGATGA TGATTTCCAG 
TTACGTTCCT TTGATCAGTT GTCACCATTA GAGAGCAATT CTCCAAGCCC TCCAAGTATG 
AGCACAGTTA CTGGGTTCCA GCAGACCCAG TTACAGAAAC CTACCATCAC TGCCACTGCC 
ACCACAACTG CCACCACTGA TGAATCAAAA ACAGAGACGA AGGACAATAA AGAAGATATT 
AAAATACTGA TTGCATCTCC ATCTTCTACC CAAGTACCTC AAGAAACGAC CACTGCTAAG 
GCATCAGCAT ACAGTGGCAC TCACAGTCGG ACAGCCTCAC CAGACAGAGC AGGAAAGAGA 
GTCATAGAAC AGACAGACAA AGCTCATCCA AGGAGCCTTA ACCTGTCTGC CACTTTGAAT 
CAAAGAAATA CTGTTCCTGA GGAAGAATTA AACCCAAAGA CAATAGCTTC GCAGAATGCT 
CAGAGGAAGC GAAAAATGGA ACATGATGGC TCCCTTTTTC AAGCAGCAGG AATTGGAACA 
TTATTGCAGC AACCAGGTGA CTGTGCACCT ACTATGTCAC TTTCCTGGAA ACGAGTGAAA 
GGATTCATAT CTAGTGAACA GAATGGAACG GAGCAAAAGA CTATTATTTT AATACCCTCC 
GATTTAGCAT GCAGACTGCT GGGGCAGTCA ATGGATGAGA GTGGATTACC ACAGCTGACC 
AGTTACGATT GTGAAGTTAA TGCTCCCATA CAAGGCAGCA GAAACCTACT GCAGGGTGAA 
GAATTACTCA GAGCTTTGGA TCAAGTTAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300849.1
CDS	177..2609
Translation	MSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001313920.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313920.1

ORF Insert Sequence:

ATGAGTTCTG AACGTCGAAA AGAAAAGTCT AGAGATGCAG CAAGATCTCG GCGAAGCAAA 
GAGTCTGAAG TTTTTTATGA GCTTGCTCAT CAGTTGCCAC TTCCCCACAA TGTGAGCTCA 
CATCTTGATA AAGCTTCTGT TATGAGGCTC ACCATCAGTT ATTTACGTGT GAGAAAACTT 
CTGGATGCCG GTGGTCTAGA CAGTGAAGAT GAGATGAAGG CACAGATGGA CTGTTTTTAT 
CTGAAAGCCC TAGATGGCTT TGTGATGGTG CTAACAGATG ACGGCGACAT GGTTTACATT 
TCTGATAACG TGAACAAATA CATGGGGTTA ACTCAGTTTG AACTAACTGG ACACAGTGTG 
TTTGATTTTA CTCATCCATG TGACCATGAG GAAATGAGAG AAATGCTTAC ACACAGAAAT 
GGCCCAGTGA GAAAAGGGAA AGAACTAAAC ACACAGCGGA GCTTTTTTCT CAGAATGAAG 
TGCACCCTAA CAAGCCGGGG GAGGACGATG AACATCAAGT CAGCAACGTG GAAGGTGCTT 
CACTGCACGG GCCATATTCA TGTCTATGAT ACCAACAGTA ACCAACCTCA GTGTGGGTAC 
AAGAAACCAC CCATGACGTG CTTGGTGCTG ATTTGTGAAC CCATTCCTCA TCCGTCAAAT 
ATTGAAATTC CTTTAGATAG CAAGACATTT CTCAGTCGAC ACAGCCTCGA TATGAAATTT 
TCTTACTGTG ATGAAAGAAT TACTGAGTTG ATGGGTTATG AGCCGGAAGA ACTTTTGGGC 
CGCTCAATTT ATGAATATTA TCATGCTTTG GATTCTGATC ATCTGACCAA AACTCACCAT 
GATATGTTTA CTAAAGGACA AGTCACCACA GGACAGTACA GGATGCTTGC CAAAAGAGGT 
GGATATGTCT GGGTTGAAAC TCAAGCAACT GTCATATATA ATACGAAGAA CTCCCAGCCA 
CAGTGCATTG TGTGTGTGAA TTATGTTGTA AGTGGTATTA TTCAGCACGA CTTGATTTTC 
TCCCTTCAAC AAACAGAATC TGTGCTCAAA CCAGTTGAAT CTTCAGATAT GAAGATGACT 
CAGCTGTTCA CCAAAGTTGA ATCAGAGGAT ACAAGCTGCC TTTTTGATAA GCTTAAGAAG 
GAGCCTGATG CTCTCACTCT GCTGGCTCCA GCTGCCGGCG ACACCATCAT CTCTCTGGAT 
TTTGGCAGCG ATGACACAGA AACTGAAGAT CAACAACTTG AAGATGTTCC ATTATATAAT 
GATGTAATGT TTCCCTCTTC TAATGAAAAA TTAAATATAA ACCTGGCAAT GTCTCCTTTA 
CCTTCATCGG AAACTCCAAA GCCACTTCGA AGTAGTGCTG ATCCTGCACT GAATCAAGAG 
GTTGCATTAA AATTAGAATC AAGTCCAGAG TCACTGGGAC TTTCTTTTAC CATGCCCCAG 
ATTCAAGATC AGCCAGCAAG TCCTTCTGAT GGAAGCACTA GACAAAGTTC ACCTGAGCCT 
AACAGTCCCA GTGAATATTG CTTTGATGTG GATAGCGATA TGGTCAATGT ATTCAAGTTG 
GAACTGGTGG AAAAACTGTT TGCTGAAGAC ACAGAGGCAA AGAATCCATT TTCAACTCAG 
GACACTGATT TAGATTTGGA GATGCTGGCT CCCTATATCC CAATGGATGA TGATTTCCAG 
TTACGTTCCT TTGATCAGTT GTCACCATTA GAGAGCAATT CTCCAAGCCC TCCAAGTATG 
AGCACAGTTA CTGGGTTCCA GCAGACCCAG TTACAGAAAC CTACCATCAC TGCCACTGCC 
ACCACAACTG CCACCACTGA TGAATCAAAA ACAGAGACGA AGGACAATAA AGAAGATATT 
AAAATACTGA TTGCATCTCC ATCTTCTACC CAAGTACCTC AAGAAACGAC CACTGCTAAG 
GCATCAGCAT ACAGTGGCAC TCACAGTCGG ACAGCCTCAC CAGACAGAGC AGGAAAGAGA 
GTCATAGAAC AGACAGACAA AGCTCATCCA AGGAGCCTTA ACCTGTCTGC CACTTTGAAT 
CAAAGAAATA CTGTTCCTGA GGAAGAATTA AACCCAAAGA CAATAGCTTC GCAGAATGCT 
CAGAGGAAGC GAAAAATGGA ACATGATGGC TCCCTTTTTC AAGCAGCAGG AATTGGAACA 
TTATTGCAGC AACCAGGTGA CTGTGCACCT ACTATGTCAC TTTCCTGGAA ACGAGTGAAA 
GGATTCATAT CTAGTGAACA GAATGGAACG GAGCAAAAGA CTATTATTTT AATACCCTCC 
GATTTAGCAT GCAGACTGCT GGGGCAGTCA ATGGATGAGA GTGGATTACC ACAGCTGACC 
AGTTACGATT GTGAAGTTAA TGCTCCCATA CAAGGCAGCA GAAACCTACT GCAGGGTGAA 
GAATTACTCA GAGCTTTGGA TCAAGTTAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_006515478.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515541.1 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47587
Clone ID Related Accession (Same CDS sequence)	XM_006515478.4 , XM_006515478.3
Accession Version	XM_006515478.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCTGA ACGTCGAAAA 
GAAAAGTCTA GAGATGCAGC AAGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAT GTGAGCTCAC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA TTTACGTGTG AGAAAACTTC TGGATGCCGG TGGTCTAGAC 
AGTGAAGATG AGATGAAGGC ACAGATGGAC TGTTTTTATC TGAAAGCCCT AGATGGCTTT 
GTGATGGTGC TAACAGATGA CGGCGACATG GTTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAAGGGAAA 
GAACTAAACA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GCACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTTC ACTGCACGGG CCATATTCAT 
GTCTATGATA CCAACAGTAA CCAACCTCAG TGTGGGTACA AGAAACCACC CATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCGTCAAATA TTGAAATTCC TTTAGATAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGAATT 
ACTGAGTTGA TGGGTTATGA GCCGGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ATTCTGATCA TCTGACCAAA ACTCACCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GATATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA TACGAAGAAC TCCCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTGCTCAAAC CAGTTGAATC TTCAGATATG AAGATGACTC AGCTGTTCAC CAAAGTTGAA 
TCAGAGGATA CAAGCTGCCT TTTTGATAAG CTTAAGAAGG AGCCTGATGC TCTCACTCTG 
CTGGCTCCAG CTGCCGGCGA CACCATCATC TCTCTGGATT TTGGCAGCGA TGACACAGAA 
ACTGAAGATC AACAACTTGA AGATGTTCCA TTATATAATG ATGTAATGTT TCCCTCTTCT 
AATGAAAAAT TAAATATAAA CCTGGCAATG TCTCCTTTAC CTTCATCGGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTAAA ATTAGAATCA 
AGTCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGC 
TTTGATGTGG ATAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAGGCAAA GAATCCATTT TCAACTCAGG ACACTGATTT AGATTTGGAG 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCAAGTATGA GCACAGTTAC TGGGTTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GCCACTGCCA CCACAACTGC CACCACTGAT 
GAATCAAAAA CAGAGACGAA GGACAATAAA GAAGATATTA AAATACTGAT TGCATCTCCA 
TCTTCTACCC AAGTACCTCA AGAAACGACC ACTGCTAAGG CATCAGCATA CAGTGGCACT 
CACAGTCGGA CAGCCTCACC AGACAGAGCA GGAAAGAGAG TCATAGAACA GACAGACAAA 
GCTCATCCAA GGAGCCTTAA CCTGTCTGCC ACTTTGAATC AAAGAAATAC TGTTCCTGAG 
GAAGAATTAA ACCCAAAGAC AATAGCTTCG CAGAATGCTC AGAGGAAGCG AAAAATGGAA 
CATGATGGCT CCCTTTTTCA AGCAGCAGGA ATTGGAACAT TATTGCAGCA ACCAGGTGAC 
TGTGCACCTA CTATGTCACT TTCCTGGAAA CGAGTGAAAG GATTCATATC TAGTGAACAG 
AATGGAACGG AGCAAAAGAC TATTATTTTA ATACCCTCCG ATTTAGCATG CAGACTGCTG 
GGGCAGTCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTACGATTG TGAAGTTAAT 
GCTCCCATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515541.1
CDS	287..2758
Translation	MEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006515478.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515478.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCTGA ACGTCGAAAA 
GAAAAGTCTA GAGATGCAGC AAGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAT GTGAGCTCAC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA TTTACGTGTG AGAAAACTTC TGGATGCCGG TGGTCTAGAC 
AGTGAAGATG AGATGAAGGC ACAGATGGAC TGTTTTTATC TGAAAGCCCT AGATGGCTTT 
GTGATGGTGC TAACAGATGA CGGCGACATG GTTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAAGGGAAA 
GAACTAAACA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GCACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTTC ACTGCACGGG CCATATTCAT 
GTCTATGATA CCAACAGTAA CCAACCTCAG TGTGGGTACA AGAAACCACC CATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCGTCAAATA TTGAAATTCC TTTAGATAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGAATT 
ACTGAGTTGA TGGGTTATGA GCCGGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ATTCTGATCA TCTGACCAAA ACTCACCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GATATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA TACGAAGAAC TCCCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTGCTCAAAC CAGTTGAATC TTCAGATATG AAGATGACTC AGCTGTTCAC CAAAGTTGAA 
TCAGAGGATA CAAGCTGCCT TTTTGATAAG CTTAAGAAGG AGCCTGATGC TCTCACTCTG 
CTGGCTCCAG CTGCCGGCGA CACCATCATC TCTCTGGATT TTGGCAGCGA TGACACAGAA 
ACTGAAGATC AACAACTTGA AGATGTTCCA TTATATAATG ATGTAATGTT TCCCTCTTCT 
AATGAAAAAT TAAATATAAA CCTGGCAATG TCTCCTTTAC CTTCATCGGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTAAA ATTAGAATCA 
AGTCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGC 
TTTGATGTGG ATAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAGGCAAA GAATCCATTT TCAACTCAGG ACACTGATTT AGATTTGGAG 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCAAGTATGA GCACAGTTAC TGGGTTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GCCACTGCCA CCACAACTGC CACCACTGAT 
GAATCAAAAA CAGAGACGAA GGACAATAAA GAAGATATTA AAATACTGAT TGCATCTCCA 
TCTTCTACCC AAGTACCTCA AGAAACGACC ACTGCTAAGG CATCAGCATA CAGTGGCACT 
CACAGTCGGA CAGCCTCACC AGACAGAGCA GGAAAGAGAG TCATAGAACA GACAGACAAA 
GCTCATCCAA GGAGCCTTAA CCTGTCTGCC ACTTTGAATC AAAGAAATAC TGTTCCTGAG 
GAAGAATTAA ACCCAAAGAC AATAGCTTCG CAGAATGCTC AGAGGAAGCG AAAAATGGAA 
CATGATGGCT CCCTTTTTCA AGCAGCAGGA ATTGGAACAT TATTGCAGCA ACCAGGTGAC 
TGTGCACCTA CTATGTCACT TTCCTGGAAA CGAGTGAAAG GATTCATATC TAGTGAACAG 
AATGGAACGG AGCAAAAGAC TATTATTTTA ATACCCTCCG ATTTAGCATG CAGACTGCTG 
GGGCAGTCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTACGATTG TGAAGTTAAT 
GCTCCCATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( NM_010431.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034561.2 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22900
Clone ID Related Accession (Same CDS sequence)	NM_010431.2
Accession Version	NM_010431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124712.5, AK034087.1, AK150367.1 and CX241289.1. On Apr 1, 2009 this sequence version replaced NM_010431.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026139.1, SRR1660815.228158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCAAG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAATGTG AGCTCACATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTATTT ACGTGTGAGA AAACTTCTGG ATGCCGGTGG TCTAGACAGT 
GAAGATGAGA TGAAGGCACA GATGGACTGT TTTTATCTGA AAGCCCTAGA TGGCTTTGTG 
ATGGTGCTAA CAGATGACGG CGACATGGTT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA AGGGAAAGAA 
CTAAACACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGCA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTTCACT GCACGGGCCA TATTCATGTC 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTACAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCG TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGAATTACT 
GAGTTGATGG GTTATGAGCC GGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGATT CTGATCATCT GACCAAAACT CACCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAATAC GAAGAACTCC CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTG 
CTCAAACCAG TTGAATCTTC AGATATGAAG ATGACTCAGC TGTTCACCAA AGTTGAATCA 
GAGGATACAA GCTGCCTTTT TGATAAGCTT AAGAAGGAGC CTGATGCTCT CACTCTGCTG 
GCTCCAGCTG CCGGCGACAC CATCATCTCT CTGGATTTTG GCAGCGATGA CACAGAAACT 
GAAGATCAAC AACTTGAAGA TGTTCCATTA TATAATGATG TAATGTTTCC CTCTTCTAAT 
GAAAAATTAA ATATAAACCT GGCAATGTCT CCTTTACCTT CATCGGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTAAAATT AGAATCAAGT 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGAGACTTC TTCAGGAAAA CGTAAACACT 
CCTAACTTTT CCCAGCCTAA CAGTCCCAGT GAATATTGCT TTGATGTGGA TAGCGATATG 
GTCAATGTAT TCAAGTTGGA ACTGGTGGAA AAACTGTTTG CTGAAGACAC AGAGGCAAAG 
AATCCATTTT CAACTCAGGA CACTGATTTA GATTTGGAGA TGCTGGCTCC CTATATCCCA 
ATGGATGATG ATTTCCAGTT ACGTTCCTTT GATCAGTTGT CACCATTAGA GAGCAATTCT 
CCAAGCCCTC CAAGTATGAG CACAGTTACT GGGTTCCAGC AGACCCAGTT ACAGAAACCT 
ACCATCACTG CCACTGCCAC CACAACTGCC ACCACTGATG AATCAAAAAC AGAGACGAAG 
GACAATAAAG AAGATATTAA AATACTGATT GCATCTCCAT CTTCTACCCA AGTACCTCAA 
GAAACGACCA CTGCTAAGGC ATCAGCATAC AGTGGCACTC ACAGTCGGAC AGCCTCACCA 
GACAGAGCAG GAAAGAGAGT CATAGAACAG ACAGACAAAG CTCATCCAAG GAGCCTTAAC 
CTGTCTGCCA CTTTGAATCA AAGAAATACT GTTCCTGAGG AAGAATTAAA CCCAAAGACA 
ATAGCTTCGC AGAATGCTCA GAGGAAGCGA AAAATGGAAC ATGATGGCTC CCTTTTTCAA 
GCAGCAGGAA TTGGAACATT ATTGCAGCAA CCAGGTGACT GTGCACCTAC TATGTCACTT 
TCCTGGAAAC GAGTGAAAGG ATTCATATCT AGTGAACAGA ATGGAACGGA GCAAAAGACT 
ATTATTTTAA TACCCTCCGA TTTAGCATGC AGACTGCTGG GGCAGTCAAT GGATGAGAGT 
GGATTACCAC AGCTGACCAG TTACGATTGT GAAGTTAATG CTCCCATACA AGGCAGCAGA 
AACCTACTGC AGGGTGAAGA ATTACTCAGA GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034561.2
CDS	424..2934
Translation	MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPERLLQENVNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_010431.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010431.2

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCAAG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAATGTG AGCTCACATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTATTT ACGTGTGAGA AAACTTCTGG ATGCCGGTGG TCTAGACAGT 
GAAGATGAGA TGAAGGCACA GATGGACTGT TTTTATCTGA AAGCCCTAGA TGGCTTTGTG 
ATGGTGCTAA CAGATGACGG CGACATGGTT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA AGGGAAAGAA 
CTAAACACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGCA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTTCACT GCACGGGCCA TATTCATGTC 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTACAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCG TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGAATTACT 
GAGTTGATGG GTTATGAGCC GGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGATT CTGATCATCT GACCAAAACT CACCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAATAC GAAGAACTCC CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTG 
CTCAAACCAG TTGAATCTTC AGATATGAAG ATGACTCAGC TGTTCACCAA AGTTGAATCA 
GAGGATACAA GCTGCCTTTT TGATAAGCTT AAGAAGGAGC CTGATGCTCT CACTCTGCTG 
GCTCCAGCTG CCGGCGACAC CATCATCTCT CTGGATTTTG GCAGCGATGA CACAGAAACT 
GAAGATCAAC AACTTGAAGA TGTTCCATTA TATAATGATG TAATGTTTCC CTCTTCTAAT 
GAAAAATTAA ATATAAACCT GGCAATGTCT CCTTTACCTT CATCGGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTAAAATT AGAATCAAGT 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGAGACTTC TTCAGGAAAA CGTAAACACT 
CCTAACTTTT CCCAGCCTAA CAGTCCCAGT GAATATTGCT TTGATGTGGA TAGCGATATG 
GTCAATGTAT TCAAGTTGGA ACTGGTGGAA AAACTGTTTG CTGAAGACAC AGAGGCAAAG 
AATCCATTTT CAACTCAGGA CACTGATTTA GATTTGGAGA TGCTGGCTCC CTATATCCCA 
ATGGATGATG ATTTCCAGTT ACGTTCCTTT GATCAGTTGT CACCATTAGA GAGCAATTCT 
CCAAGCCCTC CAAGTATGAG CACAGTTACT GGGTTCCAGC AGACCCAGTT ACAGAAACCT 
ACCATCACTG CCACTGCCAC CACAACTGCC ACCACTGATG AATCAAAAAC AGAGACGAAG 
GACAATAAAG AAGATATTAA AATACTGATT GCATCTCCAT CTTCTACCCA AGTACCTCAA 
GAAACGACCA CTGCTAAGGC ATCAGCATAC AGTGGCACTC ACAGTCGGAC AGCCTCACCA 
GACAGAGCAG GAAAGAGAGT CATAGAACAG ACAGACAAAG CTCATCCAAG GAGCCTTAAC 
CTGTCTGCCA CTTTGAATCA AAGAAATACT GTTCCTGAGG AAGAATTAAA CCCAAAGACA 
ATAGCTTCGC AGAATGCTCA GAGGAAGCGA AAAATGGAAC ATGATGGCTC CCTTTTTCAA 
GCAGCAGGAA TTGGAACATT ATTGCAGCAA CCAGGTGACT GTGCACCTAC TATGTCACTT 
TCCTGGAAAC GAGTGAAAGG ATTCATATCT AGTGAACAGA ATGGAACGGA GCAAAAGACT 
ATTATTTTAA TACCCTCCGA TTTAGCATGC AGACTGCTGG GGCAGTCAAT GGATGAGAGT 
GGATTACCAC AGCTGACCAG TTACGATTGT GAAGTTAATG CTCCCATACA AGGCAGCAGA 
AACCTACTGC AGGGTGAAGA ATTACTCAGA GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( NM_001313919.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300848.1 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu47586
Clone ID Related Accession (Same CDS sequence)	NM_001313919.1
Accession Version	NM_001313919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124712.5 and AK034087.1. On Sep 11, 2015 this sequence version replaced XM_006515477.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK034087.1, SRR1660821.151734.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCTGA ACGTCGAAAA 
GAAAAGTCTA GAGATGCAGC AAGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAT GTGAGCTCAC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA TTTACGTGTG AGAAAACTTC TGGATGCCGG TGGTCTAGAC 
AGTGAAGATG AGATGAAGGC ACAGATGGAC TGTTTTTATC TGAAAGCCCT AGATGGCTTT 
GTGATGGTGC TAACAGATGA CGGCGACATG GTTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAAGGGAAA 
GAACTAAACA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GCACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTTC ACTGCACGGG CCATATTCAT 
GTCTATGATA CCAACAGTAA CCAACCTCAG TGTGGGTACA AGAAACCACC CATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCGTCAAATA TTGAAATTCC TTTAGATAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGAATT 
ACTGAGTTGA TGGGTTATGA GCCGGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ATTCTGATCA TCTGACCAAA ACTCACCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GATATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA TACGAAGAAC TCCCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTGCTCAAAC CAGTTGAATC TTCAGATATG AAGATGACTC AGCTGTTCAC CAAAGTTGAA 
TCAGAGGATA CAAGCTGCCT TTTTGATAAG CTTAAGAAGG AGCCTGATGC TCTCACTCTG 
CTGGCTCCAG CTGCCGGCGA CACCATCATC TCTCTGGATT TTGGCAGCGA TGACACAGAA 
ACTGAAGATC AACAACTTGA AGATGTTCCA TTATATAATG ATGTAATGTT TCCCTCTTCT 
AATGAAAAAT TAAATATAAA CCTGGCAATG TCTCCTTTAC CTTCATCGGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTAAA ATTAGAATCA 
AGTCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGAGAC TTCTTCAGGA AAACGTAAAC 
ACTCCTAACT TTTCCCAGCC TAACAGTCCC AGTGAATATT GCTTTGATGT GGATAGCGAT 
ATGGTCAATG TATTCAAGTT GGAACTGGTG GAAAAACTGT TTGCTGAAGA CACAGAGGCA 
AAGAATCCAT TTTCAACTCA GGACACTGAT TTAGATTTGG AGATGCTGGC TCCCTATATC 
CCAATGGATG ATGATTTCCA GTTACGTTCC TTTGATCAGT TGTCACCATT AGAGAGCAAT 
TCTCCAAGCC CTCCAAGTAT GAGCACAGTT ACTGGGTTCC AGCAGACCCA GTTACAGAAA 
CCTACCATCA CTGCCACTGC CACCACAACT GCCACCACTG ATGAATCAAA AACAGAGACG 
AAGGACAATA AAGAAGATAT TAAAATACTG ATTGCATCTC CATCTTCTAC CCAAGTACCT 
CAAGAAACGA CCACTGCTAA GGCATCAGCA TACAGTGGCA CTCACAGTCG GACAGCCTCA 
CCAGACAGAG CAGGAAAGAG AGTCATAGAA CAGACAGACA AAGCTCATCC AAGGAGCCTT 
AACCTGTCTG CCACTTTGAA TCAAAGAAAT ACTGTTCCTG AGGAAGAATT AAACCCAAAG 
ACAATAGCTT CGCAGAATGC TCAGAGGAAG CGAAAAATGG AACATGATGG CTCCCTTTTT 
CAAGCAGCAG GAATTGGAAC ATTATTGCAG CAACCAGGTG ACTGTGCACC TACTATGTCA 
CTTTCCTGGA AACGAGTGAA AGGATTCATA TCTAGTGAAC AGAATGGAAC GGAGCAAAAG 
ACTATTATTT TAATACCCTC CGATTTAGCA TGCAGACTGC TGGGGCAGTC AATGGATGAG 
AGTGGATTAC CACAGCTGAC CAGTTACGAT TGTGAAGTTA ATGCTCCCAT ACAAGGCAGC 
AGAAACCTAC TGCAGGGTGA AGAATTACTC AGAGCTTTGG ATCAAGTTAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300848.1
CDS	424..2937
Translation	MEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPERLLQENVNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001313919.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313919.1

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCTGA ACGTCGAAAA 
GAAAAGTCTA GAGATGCAGC AAGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAT GTGAGCTCAC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA TTTACGTGTG AGAAAACTTC TGGATGCCGG TGGTCTAGAC 
AGTGAAGATG AGATGAAGGC ACAGATGGAC TGTTTTTATC TGAAAGCCCT AGATGGCTTT 
GTGATGGTGC TAACAGATGA CGGCGACATG GTTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAAGGGAAA 
GAACTAAACA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GCACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTTC ACTGCACGGG CCATATTCAT 
GTCTATGATA CCAACAGTAA CCAACCTCAG TGTGGGTACA AGAAACCACC CATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCGTCAAATA TTGAAATTCC TTTAGATAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGAATT 
ACTGAGTTGA TGGGTTATGA GCCGGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ATTCTGATCA TCTGACCAAA ACTCACCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GATATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA TACGAAGAAC TCCCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTGCTCAAAC CAGTTGAATC TTCAGATATG AAGATGACTC AGCTGTTCAC CAAAGTTGAA 
TCAGAGGATA CAAGCTGCCT TTTTGATAAG CTTAAGAAGG AGCCTGATGC TCTCACTCTG 
CTGGCTCCAG CTGCCGGCGA CACCATCATC TCTCTGGATT TTGGCAGCGA TGACACAGAA 
ACTGAAGATC AACAACTTGA AGATGTTCCA TTATATAATG ATGTAATGTT TCCCTCTTCT 
AATGAAAAAT TAAATATAAA CCTGGCAATG TCTCCTTTAC CTTCATCGGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTAAA ATTAGAATCA 
AGTCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGAGAC TTCTTCAGGA AAACGTAAAC 
ACTCCTAACT TTTCCCAGCC TAACAGTCCC AGTGAATATT GCTTTGATGT GGATAGCGAT 
ATGGTCAATG TATTCAAGTT GGAACTGGTG GAAAAACTGT TTGCTGAAGA CACAGAGGCA 
AAGAATCCAT TTTCAACTCA GGACACTGAT TTAGATTTGG AGATGCTGGC TCCCTATATC 
CCAATGGATG ATGATTTCCA GTTACGTTCC TTTGATCAGT TGTCACCATT AGAGAGCAAT 
TCTCCAAGCC CTCCAAGTAT GAGCACAGTT ACTGGGTTCC AGCAGACCCA GTTACAGAAA 
CCTACCATCA CTGCCACTGC CACCACAACT GCCACCACTG ATGAATCAAA AACAGAGACG 
AAGGACAATA AAGAAGATAT TAAAATACTG ATTGCATCTC CATCTTCTAC CCAAGTACCT 
CAAGAAACGA CCACTGCTAA GGCATCAGCA TACAGTGGCA CTCACAGTCG GACAGCCTCA 
CCAGACAGAG CAGGAAAGAG AGTCATAGAA CAGACAGACA AAGCTCATCC AAGGAGCCTT 
AACCTGTCTG CCACTTTGAA TCAAAGAAAT ACTGTTCCTG AGGAAGAATT AAACCCAAAG 
ACAATAGCTT CGCAGAATGC TCAGAGGAAG CGAAAAATGG AACATGATGG CTCCCTTTTT 
CAAGCAGCAG GAATTGGAAC ATTATTGCAG CAACCAGGTG ACTGTGCACC TACTATGTCA 
CTTTCCTGGA AACGAGTGAA AGGATTCATA TCTAGTGAAC AGAATGGAAC GGAGCAAAAG 
ACTATTATTT TAATACCCTC CGATTTAGCA TGCAGACTGC TGGGGCAGTC AATGGATGAG 
AGTGGATTAC CACAGCTGAC CAGTTACGAT TGTGAAGTTA ATGCTCCCAT ACAAGGCAGC 
AGAAACCTAC TGCAGGGTGA AGAATTACTC AGAGCTTTGG ATCAAGTTAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_006515478.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515541.1 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47587
Clone ID Related Accession (Same CDS sequence)	XM_006515478.4 , XM_006515478.3
Accession Version	XM_006515478.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515478.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCTGA ACGTCGAAAA 
GAAAAGTCTA GAGATGCAGC AAGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAT GTGAGCTCAC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA TTTACGTGTG AGAAAACTTC TGGATGCCGG TGGTCTAGAC 
AGTGAAGATG AGATGAAGGC ACAGATGGAC TGTTTTTATC TGAAAGCCCT AGATGGCTTT 
GTGATGGTGC TAACAGATGA CGGCGACATG GTTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAAGGGAAA 
GAACTAAACA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GCACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTTC ACTGCACGGG CCATATTCAT 
GTCTATGATA CCAACAGTAA CCAACCTCAG TGTGGGTACA AGAAACCACC CATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCGTCAAATA TTGAAATTCC TTTAGATAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGAATT 
ACTGAGTTGA TGGGTTATGA GCCGGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ATTCTGATCA TCTGACCAAA ACTCACCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GATATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA TACGAAGAAC TCCCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTGCTCAAAC CAGTTGAATC TTCAGATATG AAGATGACTC AGCTGTTCAC CAAAGTTGAA 
TCAGAGGATA CAAGCTGCCT TTTTGATAAG CTTAAGAAGG AGCCTGATGC TCTCACTCTG 
CTGGCTCCAG CTGCCGGCGA CACCATCATC TCTCTGGATT TTGGCAGCGA TGACACAGAA 
ACTGAAGATC AACAACTTGA AGATGTTCCA TTATATAATG ATGTAATGTT TCCCTCTTCT 
AATGAAAAAT TAAATATAAA CCTGGCAATG TCTCCTTTAC CTTCATCGGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTAAA ATTAGAATCA 
AGTCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGC 
TTTGATGTGG ATAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAGGCAAA GAATCCATTT TCAACTCAGG ACACTGATTT AGATTTGGAG 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCAAGTATGA GCACAGTTAC TGGGTTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GCCACTGCCA CCACAACTGC CACCACTGAT 
GAATCAAAAA CAGAGACGAA GGACAATAAA GAAGATATTA AAATACTGAT TGCATCTCCA 
TCTTCTACCC AAGTACCTCA AGAAACGACC ACTGCTAAGG CATCAGCATA CAGTGGCACT 
CACAGTCGGA CAGCCTCACC AGACAGAGCA GGAAAGAGAG TCATAGAACA GACAGACAAA 
GCTCATCCAA GGAGCCTTAA CCTGTCTGCC ACTTTGAATC AAAGAAATAC TGTTCCTGAG 
GAAGAATTAA ACCCAAAGAC AATAGCTTCG CAGAATGCTC AGAGGAAGCG AAAAATGGAA 
CATGATGGCT CCCTTTTTCA AGCAGCAGGA ATTGGAACAT TATTGCAGCA ACCAGGTGAC 
TGTGCACCTA CTATGTCACT TTCCTGGAAA CGAGTGAAAG GATTCATATC TAGTGAACAG 
AATGGAACGG AGCAAAAGAC TATTATTTTA ATACCCTCCG ATTTAGCATG CAGACTGCTG 
GGGCAGTCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTACGATTG TGAAGTTAAT 
GCTCCCATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515541.1
CDS	431..2902
Translation	MEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006515478.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515478.4

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCTGA ACGTCGAAAA 
GAAAAGTCTA GAGATGCAGC AAGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAT GTGAGCTCAC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA TTTACGTGTG AGAAAACTTC TGGATGCCGG TGGTCTAGAC 
AGTGAAGATG AGATGAAGGC ACAGATGGAC TGTTTTTATC TGAAAGCCCT AGATGGCTTT 
GTGATGGTGC TAACAGATGA CGGCGACATG GTTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAAGGGAAA 
GAACTAAACA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GCACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTTC ACTGCACGGG CCATATTCAT 
GTCTATGATA CCAACAGTAA CCAACCTCAG TGTGGGTACA AGAAACCACC CATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCGTCAAATA TTGAAATTCC TTTAGATAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGAATT 
ACTGAGTTGA TGGGTTATGA GCCGGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCTTTGG ATTCTGATCA TCTGACCAAA ACTCACCATG ATATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCC AAAAGAGGTG GATATGTCTG GGTTGAAACT 
CAAGCAACTG TCATATATAA TACGAAGAAC TCCCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTGCTCAAAC CAGTTGAATC TTCAGATATG AAGATGACTC AGCTGTTCAC CAAAGTTGAA 
TCAGAGGATA CAAGCTGCCT TTTTGATAAG CTTAAGAAGG AGCCTGATGC TCTCACTCTG 
CTGGCTCCAG CTGCCGGCGA CACCATCATC TCTCTGGATT TTGGCAGCGA TGACACAGAA 
ACTGAAGATC AACAACTTGA AGATGTTCCA TTATATAATG ATGTAATGTT TCCCTCTTCT 
AATGAAAAAT TAAATATAAA CCTGGCAATG TCTCCTTTAC CTTCATCGGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTAAA ATTAGAATCA 
AGTCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGC 
TTTGATGTGG ATAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAGGCAAA GAATCCATTT TCAACTCAGG ACACTGATTT AGATTTGGAG 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCAAGTATGA GCACAGTTAC TGGGTTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GCCACTGCCA CCACAACTGC CACCACTGAT 
GAATCAAAAA CAGAGACGAA GGACAATAAA GAAGATATTA AAATACTGAT TGCATCTCCA 
TCTTCTACCC AAGTACCTCA AGAAACGACC ACTGCTAAGG CATCAGCATA CAGTGGCACT 
CACAGTCGGA CAGCCTCACC AGACAGAGCA GGAAAGAGAG TCATAGAACA GACAGACAAA 
GCTCATCCAA GGAGCCTTAA CCTGTCTGCC ACTTTGAATC AAAGAAATAC TGTTCCTGAG 
GAAGAATTAA ACCCAAAGAC AATAGCTTCG CAGAATGCTC AGAGGAAGCG AAAAATGGAA 
CATGATGGCT CCCTTTTTCA AGCAGCAGGA ATTGGAACAT TATTGCAGCA ACCAGGTGAC 
TGTGCACCTA CTATGTCACT TTCCTGGAAA CGAGTGAAAG GATTCATATC TAGTGAACAG 
AATGGAACGG AGCAAAAGAC TATTATTTTA ATACCCTCCG ATTTAGCATG CAGACTGCTG 
GGGCAGTCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTACGATTG TGAAGTTAAT 
GCTCCCATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_017314961.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170450.1 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu78147
Clone ID Related Accession (Same CDS sequence)	XM_017314961.1 , XM_017314961.2
Accession Version	XM_017314961.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCAAG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAATGTG AGCTCACATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTATTT ACGTGTGAGA AAACTTCTGG ATGCCGGTGG TCTAGACAGT 
GAAGATGAGA TGAAGGCACA GATGGACTGT TTTTATCTGA AAGCCCTAGA TGGCTTTGTG 
ATGGTGCTAA CAGATGACGG CGACATGGTT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA AGGGAAAGAA 
CTAAACACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGCA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTTCACT GCACGGGCCA TATTCATGTC 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTACAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCG TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGAATTACT 
GAGTTGATGG GTTATGAGCC GGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGATT CTGATCATCT GACCAAAACT CACCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAATAC GAAGAACTCC CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTG 
CTCAAACCAG TTGAATCTTC AGATATGAAG ATGACTCAGC TGTTCACCAA AGTTGAATCA 
GAGGATACAA GCTGCCTTTT TGATAAGCTT AAGAAGGAGC CTGATGCTCT CACTCTGCTG 
GCTCCAGCTG CCGGCGACAC CATCATCTCT CTGGATTTTG GCAGCGATGA CACAGAAACT 
GAAGATCAAC AACTTGAAGA TGTTCCATTA TATAATGATG TAATGTTTCC CTCTTCTAAT 
GAAAAATTAA ATATAAACCT GGCAATGTCT CCTTTACCTT CATCGGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTAAAATT AGAATCAAGT 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTAACA GTCCCAGTGA ATATTGCTTT 
GATGTGGATA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AGGCAAAGAA TCCATTTTCA ACTCAGGACA CTGATTTAGA TTTGGAGATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCA AGTATGAGCA CAGTTACTGG GTTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGCC ACTGCCACCA CAACTGCCAC CACTGATGAA 
TCAAAAACAG AGACGAAGGA CAATAAAGAA GATATTAAAA TACTGATTGC ATCTCCATCT 
TCTACCCAAG TACCTCAAGA AACGACCACT GCTAAGGCAT CAGCATACAG TGGCACTCAC 
AGTCGGACAG CCTCACCAGA CAGAGCAGGA AAGAGAGTCA TAGAACAGAC AGACAAAGCT 
CATCCAAGGA GCCTTAACCT GTCTGCCACT TTGAATCAAA GAAATACTGT TCCTGAGGAA 
GAATTAAACC CAAAGACAAT AGCTTCGCAG AATGCTCAGA GGAAGCGAAA AATGGAACAT 
GATGGCTCCC TTTTTCAAGC AGCAGGAATT GGAACATTAT TGCAGCAACC AGGTGACTGT 
GCACCTACTA TGTCACTTTC CTGGAAACGA GTGAAAGGAT TCATATCTAG TGAACAGAAT 
GGAACGGAGC AAAAGACTAT TATTTTAATA CCCTCCGATT TAGCATGCAG ACTGCTGGGG 
CAGTCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ACGATTGTGA AGTTAATGCT 
CCCATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170450.1
CDS	284..2752
Translation	MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_017314961.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314961.1

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCAAG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAATGTG AGCTCACATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTATTT ACGTGTGAGA AAACTTCTGG ATGCCGGTGG TCTAGACAGT 
GAAGATGAGA TGAAGGCACA GATGGACTGT TTTTATCTGA AAGCCCTAGA TGGCTTTGTG 
ATGGTGCTAA CAGATGACGG CGACATGGTT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA AGGGAAAGAA 
CTAAACACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGCA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTTCACT GCACGGGCCA TATTCATGTC 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTACAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCG TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGAATTACT 
GAGTTGATGG GTTATGAGCC GGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGATT CTGATCATCT GACCAAAACT CACCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAATAC GAAGAACTCC CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTG 
CTCAAACCAG TTGAATCTTC AGATATGAAG ATGACTCAGC TGTTCACCAA AGTTGAATCA 
GAGGATACAA GCTGCCTTTT TGATAAGCTT AAGAAGGAGC CTGATGCTCT CACTCTGCTG 
GCTCCAGCTG CCGGCGACAC CATCATCTCT CTGGATTTTG GCAGCGATGA CACAGAAACT 
GAAGATCAAC AACTTGAAGA TGTTCCATTA TATAATGATG TAATGTTTCC CTCTTCTAAT 
GAAAAATTAA ATATAAACCT GGCAATGTCT CCTTTACCTT CATCGGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTAAAATT AGAATCAAGT 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTAACA GTCCCAGTGA ATATTGCTTT 
GATGTGGATA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AGGCAAAGAA TCCATTTTCA ACTCAGGACA CTGATTTAGA TTTGGAGATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCA AGTATGAGCA CAGTTACTGG GTTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGCC ACTGCCACCA CAACTGCCAC CACTGATGAA 
TCAAAAACAG AGACGAAGGA CAATAAAGAA GATATTAAAA TACTGATTGC ATCTCCATCT 
TCTACCCAAG TACCTCAAGA AACGACCACT GCTAAGGCAT CAGCATACAG TGGCACTCAC 
AGTCGGACAG CCTCACCAGA CAGAGCAGGA AAGAGAGTCA TAGAACAGAC AGACAAAGCT 
CATCCAAGGA GCCTTAACCT GTCTGCCACT TTGAATCAAA GAAATACTGT TCCTGAGGAA 
GAATTAAACC CAAAGACAAT AGCTTCGCAG AATGCTCAGA GGAAGCGAAA AATGGAACAT 
GATGGCTCCC TTTTTCAAGC AGCAGGAATT GGAACATTAT TGCAGCAACC AGGTGACTGT 
GCACCTACTA TGTCACTTTC CTGGAAACGA GTGAAAGGAT TCATATCTAG TGAACAGAAT 
GGAACGGAGC AAAAGACTAT TATTTTAATA CCCTCCGATT TAGCATGCAG ACTGCTGGGG 
CAGTCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ACGATTGTGA AGTTAATGCT 
CCCATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_017314961.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170450.1 Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu78147
Clone ID Related Accession (Same CDS sequence)	XM_017314961.1 , XM_017314961.2
Accession Version	XM_017314961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCAAG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAATGTG AGCTCACATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTATTT ACGTGTGAGA AAACTTCTGG ATGCCGGTGG TCTAGACAGT 
GAAGATGAGA TGAAGGCACA GATGGACTGT TTTTATCTGA AAGCCCTAGA TGGCTTTGTG 
ATGGTGCTAA CAGATGACGG CGACATGGTT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA AGGGAAAGAA 
CTAAACACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGCA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTTCACT GCACGGGCCA TATTCATGTC 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTACAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCG TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGAATTACT 
GAGTTGATGG GTTATGAGCC GGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGATT CTGATCATCT GACCAAAACT CACCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAATAC GAAGAACTCC CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTG 
CTCAAACCAG TTGAATCTTC AGATATGAAG ATGACTCAGC TGTTCACCAA AGTTGAATCA 
GAGGATACAA GCTGCCTTTT TGATAAGCTT AAGAAGGAGC CTGATGCTCT CACTCTGCTG 
GCTCCAGCTG CCGGCGACAC CATCATCTCT CTGGATTTTG GCAGCGATGA CACAGAAACT 
GAAGATCAAC AACTTGAAGA TGTTCCATTA TATAATGATG TAATGTTTCC CTCTTCTAAT 
GAAAAATTAA ATATAAACCT GGCAATGTCT CCTTTACCTT CATCGGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTAAAATT AGAATCAAGT 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTAACA GTCCCAGTGA ATATTGCTTT 
GATGTGGATA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AGGCAAAGAA TCCATTTTCA ACTCAGGACA CTGATTTAGA TTTGGAGATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCA AGTATGAGCA CAGTTACTGG GTTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGCC ACTGCCACCA CAACTGCCAC CACTGATGAA 
TCAAAAACAG AGACGAAGGA CAATAAAGAA GATATTAAAA TACTGATTGC ATCTCCATCT 
TCTACCCAAG TACCTCAAGA AACGACCACT GCTAAGGCAT CAGCATACAG TGGCACTCAC 
AGTCGGACAG CCTCACCAGA CAGAGCAGGA AAGAGAGTCA TAGAACAGAC AGACAAAGCT 
CATCCAAGGA GCCTTAACCT GTCTGCCACT TTGAATCAAA GAAATACTGT TCCTGAGGAA 
GAATTAAACC CAAAGACAAT AGCTTCGCAG AATGCTCAGA GGAAGCGAAA AATGGAACAT 
GATGGCTCCC TTTTTCAAGC AGCAGGAATT GGAACATTAT TGCAGCAACC AGGTGACTGT 
GCACCTACTA TGTCACTTTC CTGGAAACGA GTGAAAGGAT TCATATCTAG TGAACAGAAT 
GGAACGGAGC AAAAGACTAT TATTTTAATA CCCTCCGATT TAGCATGCAG ACTGCTGGGG 
CAGTCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ACGATTGTGA AGTTAATGCT 
CCCATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170450.1
CDS	451..2919
Translation	MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_017314961.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hypoxia inducible factor 1, alpha subunit (Hif1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314961.2

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCAAG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAATGTG AGCTCACATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTATTT ACGTGTGAGA AAACTTCTGG ATGCCGGTGG TCTAGACAGT 
GAAGATGAGA TGAAGGCACA GATGGACTGT TTTTATCTGA AAGCCCTAGA TGGCTTTGTG 
ATGGTGCTAA CAGATGACGG CGACATGGTT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA AGGGAAAGAA 
CTAAACACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGCA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTTCACT GCACGGGCCA TATTCATGTC 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTACAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCG TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGAATTACT 
GAGTTGATGG GTTATGAGCC GGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGATT CTGATCATCT GACCAAAACT CACCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAATAC GAAGAACTCC CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTG 
CTCAAACCAG TTGAATCTTC AGATATGAAG ATGACTCAGC TGTTCACCAA AGTTGAATCA 
GAGGATACAA GCTGCCTTTT TGATAAGCTT AAGAAGGAGC CTGATGCTCT CACTCTGCTG 
GCTCCAGCTG CCGGCGACAC CATCATCTCT CTGGATTTTG GCAGCGATGA CACAGAAACT 
GAAGATCAAC AACTTGAAGA TGTTCCATTA TATAATGATG TAATGTTTCC CTCTTCTAAT 
GAAAAATTAA ATATAAACCT GGCAATGTCT CCTTTACCTT CATCGGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTAAAATT AGAATCAAGT 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGTTCACCT GAGCCTAACA GTCCCAGTGA ATATTGCTTT 
GATGTGGATA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AGGCAAAGAA TCCATTTTCA ACTCAGGACA CTGATTTAGA TTTGGAGATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCA AGTATGAGCA CAGTTACTGG GTTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGCC ACTGCCACCA CAACTGCCAC CACTGATGAA 
TCAAAAACAG AGACGAAGGA CAATAAAGAA GATATTAAAA TACTGATTGC ATCTCCATCT 
TCTACCCAAG TACCTCAAGA AACGACCACT GCTAAGGCAT CAGCATACAG TGGCACTCAC 
AGTCGGACAG CCTCACCAGA CAGAGCAGGA AAGAGAGTCA TAGAACAGAC AGACAAAGCT 
CATCCAAGGA GCCTTAACCT GTCTGCCACT TTGAATCAAA GAAATACTGT TCCTGAGGAA 
GAATTAAACC CAAAGACAAT AGCTTCGCAG AATGCTCAGA GGAAGCGAAA AATGGAACAT 
GATGGCTCCC TTTTTCAAGC AGCAGGAATT GGAACATTAT TGCAGCAACC AGGTGACTGT 
GCACCTACTA TGTCACTTTC CTGGAAACGA GTGAAAGGAT TCATATCTAG TGAACAGAAT 
GGAACGGAGC AAAAGACTAT TATTTTAATA CCCTCCGATT TAGCATGCAG ACTGCTGGGG 
CAGTCAATGG ATGAGAGTGG ATTACCACAG CTGACCAGTT ACGATTGTGA AGTTAATGCT 
CCCATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT TACTCAGAGC TTTGGATCAA 
GTTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Hif1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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