Heph cDNA ORF clone, Mus musculus(house mouse)

The following Heph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Heph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu07224 NM_181273.4
Latest version!
Mus musculus hephaestin (Heph), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $496.30
$709.00
OMu06138 NM_001159627.1
Latest version!
Mus musculus hephaestin (Heph), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu06138 NM_010417.2
Latest version!
Mus musculus hephaestin (Heph), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu07655 NM_001159628.1
Latest version!
Mus musculus hephaestin (Heph), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu07224
    Clone ID Related Accession (Same CDS sequence) NM_181273.4
    Accession Version NM_181273.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Mus musculus(house mouse)
    Product hephaestin isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ053588.1 and AK081330.1. On Apr 25, 2009 this sequence version replaced NM_181273.3. Transcript Variant: This variant (2) represents use of an alternate promoter and uses an alternate 3' splice pattern, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK081330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GATCCCACAA GAGATACAAA TTCTGGCCTG GTGGGCCCTC TACTGGTGTG CAAGGCTGGG
    GCCTTGGGTG CAGATGGCAA GCAGAAAGGA GTGGATAAAG AATTTTTTCT CCTCTTCACT
    GTGTTTGATG AGAATGAGAG CTGGTACAAC AATGCCAATC AGGCAGCTGG TATGTTGGAT
    TCCCGACTGC TCTCAGAGGA TGTCGAGGGC TTCCAGGACT CCAATCGAAT GCATGCTATT
    AATGGATTTC TGTTCTCTAA CCTGCCCAGG CTGGACATGT GCAAGGGTGA TACTGTGGCC
    TGGCACCTGC TTGGCCTGGG CACAGAGACT GATGTACATG GGGTAATGTT CGAGGGCAAC
    ACTGTGCAGC TTCAGGGCAT GAGGAAAGGT GCAGTCATGC TCTTTCCTCA CACCTTTGTG
    ACGGCCATCA TGCAGCCTGA CAATCCTGGA ATATTTGAAA TCTACTGCCA AGCAGGCAGC
    CACCGAGAGG AAGGGATGCA GGCAATTTAT AATGTCTCTC AGTGTTCTAG TCATCAAGAC
    AGCCCACGCC AACACTACCA AGCTTCAAGA GTCTACTATA TCATGGCAGA AGAGATAGAG
    TGGGATTACT GCCCTGATAG AAGCTGGGAA CTGGAATGGC ATAACACATC TGAGAAAGAC
    AGCTATGGCC ATGTTTTCCT GAGCAATAAA GATGGGCTCC TGGGTTCCAA ATATAAGAAA
    GCTGTATTCA GGGAATACAC TGATGGTACT TTCAGAATAC CTCGGCCAAG GTCTGGACCA
    GAGGAGCACT TGGGAATCCT GGGTCCACTT ATCAGAGGAG AGGTTGGTGA TATCTTGACT
    GTAGTGTTCA AGAATAAGGC CAGTCGACCA TATTCTATAC ATGCCCATGG AGTTCTAGAA
    TCTAACACTG GCGGGCCACA GGCTGCTGAG CCTGGTGAGT TGGAGCACTT AATGAAGAGA
    CAAAGGCTTT ACAATCCTTT TACTTTAGTC TTCTGGTTTA TTCCTTTCAA CATATTACAT
    TCTTGGGCTG GGCAGGTCAC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851790.1
    CDS500..3043
    Translation

    Target ORF information:

    RefSeq Version NM_181273.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus hephaestin (Heph), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181273.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GATCCCACAA GAGATACAAA TTCTGGCCTG GTGGGCCCTC TACTGGTGTG CAAGGCTGGG
    GCCTTGGGTG CAGATGGCAA GCAGAAAGGA GTGGATAAAG AATTTTTTCT CCTCTTCACT
    GTGTTTGATG AGAATGAGAG CTGGTACAAC AATGCCAATC AGGCAGCTGG TATGTTGGAT
    TCCCGACTGC TCTCAGAGGA TGTCGAGGGC TTCCAGGACT CCAATCGAAT GCATGCTATT
    AATGGATTTC TGTTCTCTAA CCTGCCCAGG CTGGACATGT GCAAGGGTGA TACTGTGGCC
    TGGCACCTGC TTGGCCTGGG CACAGAGACT GATGTACATG GGGTAATGTT CGAGGGCAAC
    ACTGTGCAGC TTCAGGGCAT GAGGAAAGGT GCAGTCATGC TCTTTCCTCA CACCTTTGTG
    ACGGCCATCA TGCAGCCTGA CAATCCTGGA ATATTTGAAA TCTACTGCCA AGCAGGCAGC
    CACCGAGAGG AAGGGATGCA GGCAATTTAT AATGTCTCTC AGTGTTCTAG TCATCAAGAC
    AGCCCACGCC AACACTACCA AGCTTCAAGA GTCTACTATA TCATGGCAGA AGAGATAGAG
    TGGGATTACT GCCCTGATAG AAGCTGGGAA CTGGAATGGC ATAACACATC TGAGAAAGAC
    AGCTATGGCC ATGTTTTCCT GAGCAATAAA GATGGGCTCC TGGGTTCCAA ATATAAGAAA
    GCTGTATTCA GGGAATACAC TGATGGTACT TTCAGAATAC CTCGGCCAAG GTCTGGACCA
    GAGGAGCACT TGGGAATCCT GGGTCCACTT ATCAGAGGAG AGGTTGGTGA TATCTTGACT
    GTAGTGTTCA AGAATAAGGC CAGTCGACCA TATTCTATAC ATGCCCATGG AGTTCTAGAA
    TCTAACACTG GCGGGCCACA GGCTGCTGAG CCTGGTGAGT TGGAGCACTT AATGAAGAGA
    CAAAGGCTTT ACAATCCTTT TACTTTAGTC TTCTGGTTTA TTCCTTTCAA CATATTACAT
    TCTTGGGCTG GGCAGGTCAC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu06138
    Clone ID Related Accession (Same CDS sequence) NM_010417.2 , NM_001159627.1
    Accession Version NM_010417.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product hephaestin isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ067085.1, AF082567.1 and AK129194.1. On Apr 25, 2009 this sequence version replaced NM_010417.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF082567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT
    CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT
    GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG
    GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC
    TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG
    TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT
    GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT
    GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG
    GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC
    TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA
    GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT
    CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG
    ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG
    AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT
    AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT
    GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC
    TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT
    CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG
    TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT
    TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC
    ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT
    GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA
    ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC
    AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC
    CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT
    GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG
    GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA
    TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG
    GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA
    GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC
    ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC
    ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC
    AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_034547.2
    CDS508..3981
    Translation

    Target ORF information:

    RefSeq Version NM_010417.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus hephaestin (Heph), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_010417.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT
    CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT
    GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG
    GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC
    TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG
    TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT
    GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT
    GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG
    GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC
    TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA
    GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT
    CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG
    ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG
    AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT
    AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT
    GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC
    TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT
    CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG
    TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT
    TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC
    ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT
    GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA
    ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC
    AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC
    CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT
    GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG
    GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA
    TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG
    GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA
    GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC
    ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC
    ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC
    AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu06138
    Clone ID Related Accession (Same CDS sequence) NM_010417.2 , NM_001159627.1
    Accession Version NM_001159627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product hephaestin isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ053588.1, AK129194.1 and AF082567.1. Transcript Variant: This variant (3) represents use of an alternate promoter, compared to variant 1, and enocdes the longest isoform (1). Both variants 1 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129194.1, SRR1660821.102849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT
    CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT
    GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG
    GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC
    TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG
    TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT
    GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT
    GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG
    GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC
    TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA
    GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT
    CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG
    ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG
    AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT
    AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT
    GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC
    TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT
    CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG
    TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT
    TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC
    ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT
    GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA
    ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC
    AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC
    CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT
    GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG
    GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA
    TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG
    GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA
    GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC
    ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC
    ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC
    AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153099.1
    CDS500..3973
    Translation

    Target ORF information:

    RefSeq Version NM_001159627.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus hephaestin (Heph), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001159627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT
    CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT
    GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG
    GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC
    TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG
    TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT
    GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT
    GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG
    GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC
    TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA
    GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT
    CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG
    ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG
    AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT
    AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT
    GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC
    TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT
    CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG
    TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT
    TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC
    ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT
    GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA
    ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC
    AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC
    CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT
    GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG
    GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA
    TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG
    GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA
    GCAGTGATTC TAAGGGACAT TGGAGGTGAC AATGTGAAGA TGCTGGGCAT GAACATTCCC
    ATAAAGGATG TAGAGATTCT GTCTTCTGCT TTGATTGCCA TATGTGTGCT TCTGTTGCTC
    ATTGCTCTGG CTCTTGGTGG TGTAGTCTGG TACCAGCATC GACAAAGAAA GCTTCGGCGC
    AACAGGAGGT CCATTCTTGA TGATAGCTTC AAGCTTCTCT CTCTCAAGCA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07655
    Clone ID Related Accession (Same CDS sequence) NM_001159628.1
    Accession Version NM_001159628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product hephaestin isoform 3 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732365.17. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (3) lacks one internal amino acid near the C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT
    CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT
    GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG
    GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC
    TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG
    TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT
    GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT
    GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG
    GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC
    TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA
    GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT
    CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG
    ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG
    AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT
    AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT
    GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC
    TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT
    CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG
    TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT
    TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC
    ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT
    GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA
    ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC
    AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC
    CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT
    GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG
    GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA
    TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG
    GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA
    GTGATTCTAA GGGACATTGG AGGTGACAAT GTGAAGATGC TGGGCATGAA CATTCCCATA
    AAGGATGTAG AGATTCTGTC TTCTGCTTTG ATTGCCATAT GTGTGCTTCT GTTGCTCATT
    GCTCTGGCTC TTGGTGGTGT AGTCTGGTAC CAGCATCGAC AAAGAAAGCT TCGGCGCAAC
    AGGAGGTCCA TTCTTGATGA TAGCTTCAAG CTTCTCTCTC TCAAGCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001153100.1
    CDS508..3978
    Translation

    Target ORF information:

    RefSeq Version NM_001159628.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus hephaestin (Heph), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001159628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGGCAG GCCATCTTCT CTGGGCTTTA CTGTTGATGC ACTCCTTGTG GTCTATACCA 
    ACTGATGGGG CCATTCGAAA CTACTACTTG GGCATCCAGG ATATGCAGTG GAACTATGCT
    CCCAAAGGAA GAAATGTCAT CACAAATCAG ACTCTCAACA ATGACACAGT GGCTTCCAGT
    TTCCTGAAGT CTGGCAAAAA CAGGATAGGG AGTAGTTACA AGAAGACTGT TTATAAGGAA
    TACAGTGATG GCACATACAC TGAAGAAATA GCCAAGCCTG CCTGGTTGGG CTTCCTAGGA
    CCACTGTTAC AGGCTGAGGT GGGGGATGTC ATCTTGATTC ACCTGAAGAA TTTTGCGAGC
    CGACCTTACA CCATTCACCC TCACGGTGTT TTTTATGAGA AGGACTCAGA AGGCTCACTA
    TACCCAGATG GTTCTTCTGG GTATCTGAAA GCGGATGATT CTGTCCCCCC TGGGGGCAGC
    CATGTCTACA ACTGGAGTAT TCCAGAAAGT CATGCCCCCA CTGAGGCAGA CCCAGCATGC
    CTCACCTGGA TTTACCACTC GCATGTAGAT GCTCCAAGAG ACATTGCAAC TGGTCTCATT
    GGACCTCTTA TCACCTGTAA AAGAGGGACC CTGGATGGTA ATTCCCCACC TCAGAGGAAG
    GATGTGGACC ATAATTTCTT CCTCCTCTTC AGTGTGATAG ATGAGAACCT TAGCTGGCAC
    CTTGATGACA ACATTGCTAC TTACTGCTCA GACCCTGCCT CGGTGGACAA AGAAGATGGA
    GCCTTTCAAG ACAGCAACAG GATGCATGCA ATCAATGGGT TTGTCTTTGG GAACTTACCA
    GAGTTGAGCA TGTGTGCACA GAAGCATGTG GCCTGGCACT TGTTTGGCAT GGGCAATGAA
    ATAGATGTCC ACACAGCTTT CTTCCATGGA CAGATGCTGA GTATCCGTGG ACACCACACT
    GATGTTGCAA ACATTTTTCC AGCTACCTTT GTGACTGCTG AGATGGTGCC CCAAAAGTCT
    GGAACCTGGC TAATTAGCTG TGAAGTGAAC AGCCACTTGA GAAGTGGCAT GCAGGCCTTC
    TACAAGGTTG ACTCTTGCTC CATGGACCCA CCTGTGGACC AGCTCACTGG CAAAGTTCGT
    CAGTACTTCA TTCAGGCCCA TGAGATTCAA TGGGACTATG GTCCAATAGG GTATGATGGC
    AGAACTGGGA AGAGTTTGAG AGAGCCAGGA AGTGGCCCAG ATAAGTACTT CCAGAAGAGC
    TCTAGTCGAA TTGGAGGTAC TTACTGGAAA GTTCGATATG AAGCCTTTCA AGATGAGACA
    TTCCAGGAAA GGGTACATCA GGAAGAAGAA ACACATCTTG GAATACTGGG ACCAGTGATA
    AGGGCTGAAG TGGGTGACAC CATCCAGGTG GTCTTCTATA ACCGTGCCTC CCAGCCATTC
    AGCATACAGC CCCATGGTGT CTTTTATGAG AAAAACTCTG AGGGCACCGT GTACAATGAT
    GGCACATCTC ATCCCAAAGT AGCCAAGTCA TTTGAAAAAG TCACATACTA CTGGACGGTT
    CCTCCCCATG CTGGGCCCAC TGCTCAGGAT CCTGCCTGTC TAACCTGGAT GTACTTCTCT
    GCTGCAGATC CCACAAGAGA TACAAATTCT GGCCTGGTGG GCCCTCTACT GGTGTGCAAG
    GCTGGGGCCT TGGGTGCAGA TGGCAAGCAG AAAGGAGTGG ATAAAGAATT TTTTCTCCTC
    TTCACTGTGT TTGATGAGAA TGAGAGCTGG TACAACAATG CCAATCAGGC AGCTGGTATG
    TTGGATTCCC GACTGCTCTC AGAGGATGTC GAGGGCTTCC AGGACTCCAA TCGAATGCAT
    GCTATTAATG GATTTCTGTT CTCTAACCTG CCCAGGCTGG ACATGTGCAA GGGTGATACT
    GTGGCCTGGC ACCTGCTTGG CCTGGGCACA GAGACTGATG TACATGGGGT AATGTTCGAG
    GGCAACACTG TGCAGCTTCA GGGCATGAGG AAAGGTGCAG TCATGCTCTT TCCTCACACC
    TTTGTGACGG CCATCATGCA GCCTGACAAT CCTGGAATAT TTGAAATCTA CTGCCAAGCA
    GGCAGCCACC GAGAGGAAGG GATGCAGGCA ATTTATAATG TCTCTCAGTG TTCTAGTCAT
    CAAGACAGCC CACGCCAACA CTACCAAGCT TCAAGAGTCT ACTATATCAT GGCAGAAGAG
    ATAGAGTGGG ATTACTGCCC TGATAGAAGC TGGGAACTGG AATGGCATAA CACATCTGAG
    AAAGACAGCT ATGGCCATGT TTTCCTGAGC AATAAAGATG GGCTCCTGGG TTCCAAATAT
    AAGAAAGCTG TATTCAGGGA ATACACTGAT GGTACTTTCA GAATACCTCG GCCAAGGTCT
    GGACCAGAGG AGCACTTGGG AATCCTGGGT CCACTTATCA GAGGAGAGGT TGGTGATATC
    TTGACTGTAG TGTTCAAGAA TAAGGCCAGT CGACCATATT CTATACATGC CCATGGAGTT
    CTAGAATCTA ACACTGGCGG GCCACAGGCT GCTGAGCCTG GTGAAGTACT TACTTACCAG
    TGGAACATCC CAGAAAGATC TGGTCCTGGT CCTAGTGACT CTGCTTGTGT TTCCTGGATT
    TATTATTCTG CAGTGGATCC CATCAAGGAC ATGTATAGTG GTCTGGTTGG ACCCCTAGTC
    ATCTGCAGAA ATGGTATCTT GGAACCCAAT GGAGGCCGGA ATGATATGGA CCGGGAATTT
    GCCTTGTTGT TTTTGATCTT TGATGAGAAC CAATCTTGGT ATCTGAAGGA GAATATTGCA
    ACATATGGAC CTCAAGAATC AAGTCATGTT AACTTGAAGG ATGCCACCTT CCTAGAGAGC
    AATAAAATGC ATGCTATCAA TGGGAAACTC TATGCAAACC TCAGGGGTCT TACTGTATAC
    CAAGGAGAAC GAGTAGCCTG GTACATGCTA GCCATGGGCC AAGATACTGA CATTCACACT
    GTACACTTCC ATGCAGAGAG TTTCCTCTAT CAGAATGGGC AAAGTTACAG GGCAGATGTG
    GTGGATCTCT TCCCAGGAAC ATTTGAAGTT GTGGAGATGG TAGCCAGCAA CCCTGGGACA
    TGGCTGATGC ACTGCCATGT GACTGACCAT GTTCATGCTG GCATGGAGAC CATCTTTACG
    GTCTTGTCTC ATGAAGAACA TTTCAGCACT ATGACCACTA TTACTAAAGA GATTGGAAAA
    GTGATTCTAA GGGACATTGG AGGTGACAAT GTGAAGATGC TGGGCATGAA CATTCCCATA
    AAGGATGTAG AGATTCTGTC TTCTGCTTTG ATTGCCATAT GTGTGCTTCT GTTGCTCATT
    GCTCTGGCTC TTGGTGGTGT AGTCTGGTAC CAGCATCGAC AAAGAAAGCT TCGGCGCAAC
    AGGAGGTCCA TTCTTGATGA TAGCTTCAAG CTTCTCTCTC TCAAGCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.