H3f3b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H3f3b
Entrez Gene ID	15081
Full Name	H3 histone, family 3B
Synonyms	9430068D06Rik,H3.3B
General protein information	Preferred Names H3 histone, family 3B Names histone H3.3 H3L-like histone
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532248.2	XP_006532311.1	histone H3.3 isoform X1
XM_006532248.3	XP_006532311.1	histone H3.3 isoform X1
NM_008211.3	NP_032237.1	histone H3.3

Pathways from BioSystems

Homologies

Homo sapiens (human)	H3F3B	NP_005315.1
Mus musculus (house mouse)	H3f3b	NP_032237.1
Gallus gallus (chicken)	H3F3B	NP_990627.1
Pan troglodytes (chimpanzee)	H3F3B	XP_003315784.1
Pan troglodytes (chimpanzee)	H3F3A	XP_003339055.1
	LOC707040	XP_001105140.1
Rattus norvegicus (Norway rat)	H3f3b	NP_446437.1
Drosophila melanogaster (fruit fly)	His3:CG33857	NP_001027373.1
Homo sapiens (human)	H3F3A	NP_002098.1
Mus musculus (house mouse)	H3f3a	NP_032236.1
Gallus gallus (chicken)	LOC100859629	XP_003643522.1
Xenopus tropicalis (tropical clawed frog)	LOC101730642	XP_004913911.1
Canis lupus familiaris (dog)	H3F3B	XP_537232.1
Bos taurus (cattle)	H3F3A	NP_001014411.1
Bos taurus (cattle)	H3F3B	NP_001229500.1
Pan troglodytes (chimpanzee)	HIST1H3A	NP_001092038.1
Rattus norvegicus (Norway rat)	LOC100361558	XP_002728089.1
Danio rerio (zebrafish)	LOC565028	XP_693431.3

Gene Ontology
Bibliography

Related articles in PubMed

Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos.
Kong Q, Banaszynski LA, Geng F, Zhang X, Zhang J, Zhang H, O'Neill CL, Yan P, Liu Z, Shido K, Palermo GD, Allis CD, Rafii S, Rosenwaks Z, Wen D
The Journal of biological chemistry(2018 Jan)

Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain.
Xia W, Jiao J
Cell death and differentiation24(9)1548-1563(2017 Sep)

Aberrant H3.3 dynamics in NAc promote vulnerability to depressive-like behavior.
Lepack AE, Bagot RC, Pe?a CJ, Loh YE, Farrelly LA, Lu Y, Powell SK, Lorsch ZS, Issler O, Cates HM, Tamminga CA, Molina H, Shen L, Nestler EJ, Allis CD, Maze I
Proceedings of the National Academy of Sciences of the United States of America113(44)12562-12567(2016 Nov)

Histone variant H3.3 provides the heterochromatic H3 lysine 9 tri-methylation mark at telomeres.
Udugama M, M Chang FT, Chan FL, Tang MC, Pickett HA, R McGhie JD, Mayne L, Collas P, Mann JR, Wong LH
Nucleic acids research43(21)10227-37(2015 Dec)

Tissue-specific expression of histone H3 variants diversified after species separation.
Maehara K, Harada A, Sato Y, Matsumoto M, Nakayama KI, Kimura H, Ohkawa Y
Epigenetics & chromatin835(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In H3f3a and H3f3b null mouse embryonic stem cells, H3.3 deficiency results in reduced levels of H3K9me3 level, H4K20me3 and ATRX at the telomeres, accompanied with an increase in telomeric transcription.
Title: Histone variant H3.3 provides the heterochromatic H3 lysine 9 tri-methylation mark at telomeres.

histone H3 methylation plays a role in T cell differentiation into the natural killer T cell lineage.
Title: Coupling of T cell receptor specificity to natural killer T cell development by bivalent histone H3 methylation.

H3.3 is a crucial maternal factor for oocyte reprogramming
Title: Histone variant H3.3 is an essential maternal factor for oocyte reprogramming.

Results demonstrate a role for a modifiable residue within a histone-variant-specific context during reprogramming and identifies a novel function for mammalian H3.3 in the initial formation of dsRNA-dependent heterochromatin.
Title: Heterochromatin formation in the mouse embryo requires critical residues of the histone variant H3.3.

Results support the hypothesis that histone H3.3 is incorporated after disruption of nucleosomes mediated by transcription elongation.
Title: Histone H3.3 deposition at E2F-regulated genes is linked to transcription.

The following H3f3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H3f3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22067	NM_008211.3 Latest version!	Mus musculus H3.3 histone B (H3f3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu22067	XM_006532248.3 Latest version!	Mus musculus H3.3 histone B (H3f3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu22067	XM_006532248.2	Mus musculus H3 histone, family 3B (H3f3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H3f3b ( NM_008211.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032237.1 Mus musculus H3.3 histone B (H3f3b), mRNA.

Price & Availability↑

CloneID	OMu22067
Clone ID Related Accession (Same CDS sequence)	XM_006532248.2 , XM_006532248.3 , NM_008211.3
Accession Version	NM_008211.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	histone H3.3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK153530.1, AK161675.1, AK151336.1, BY685958.1 and AV112938.1. On Oct 13, 2006 this sequence version replaced NM_008211.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151222.1, AK011431.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 25675407 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGAA CCAAGCAGAC CGCTAGGAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACCA AGGCGGCTCG GAAAAGCGCG CCCTCTACCG GCGGGGTGAA GAAGCCTCAC 
CGCTACAGGC CAGGGACCGT GGCTCTGAGA GAGATCCGTC GTTACCAGAA ATCGACTGAG 
CTGCTCATCC GGAAGCTGCC ATTCCAGAGA TTGGTGAGGG AGATCGCCCA GGATTTCAAA 
ACCGACTTGA GGTTTCAAAG TGCAGCCATC GGTGCCCTTC AGGAGGCTAG CGAAGCATAC 
CTGGTGGGGT TGTTTGAAGA TACCAATCTG TGTGCCATCC ACGCCAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGGG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032237.1
CDS	137..547
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_008211.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus H3.3 histone B (H3f3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008211.3

ORF Insert Sequence:

ATGGCCCGAA CCAAGCAGAC CGCTAGGAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACCA AGGCGGCTCG GAAAAGCGCG CCCTCTACCG GCGGGGTGAA GAAGCCTCAC 
CGCTACAGGC CAGGGACCGT GGCTCTGAGA GAGATCCGTC GTTACCAGAA ATCGACTGAG 
CTGCTCATCC GGAAGCTGCC ATTCCAGAGA TTGGTGAGGG AGATCGCCCA GGATTTCAAA 
ACCGACTTGA GGTTTCAAAG TGCAGCCATC GGTGCCCTTC AGGAGGCTAG CGAAGCATAC 
CTGGTGGGGT TGTTTGAAGA TACCAATCTG TGTGCCATCC ACGCCAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGGG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H3f3b ( XM_006532248.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532311.1 Mus musculus H3 histone, family 3B (H3f3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22067
Clone ID Related Accession (Same CDS sequence)	XM_006532248.2 , XM_006532248.3 , NM_008211.3
Accession Version	XM_006532248.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histone H3.3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGAA CCAAGCAGAC CGCTAGGAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACCA AGGCGGCTCG GAAAAGCGCG CCCTCTACCG GCGGGGTGAA GAAGCCTCAC 
CGCTACAGGC CAGGGACCGT GGCTCTGAGA GAGATCCGTC GTTACCAGAA ATCGACTGAG 
CTGCTCATCC GGAAGCTGCC ATTCCAGAGA TTGGTGAGGG AGATCGCCCA GGATTTCAAA 
ACCGACTTGA GGTTTCAAAG TGCAGCCATC GGTGCCCTTC AGGAGGCTAG CGAAGCATAC 
CTGGTGGGGT TGTTTGAAGA TACCAATCTG TGTGCCATCC ACGCCAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGGG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532311.1
CDS	497..907
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	XM_006532248.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus H3 histone, family 3B (H3f3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532248.2

ORF Insert Sequence:

ATGGCCCGAA CCAAGCAGAC CGCTAGGAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACCA AGGCGGCTCG GAAAAGCGCG CCCTCTACCG GCGGGGTGAA GAAGCCTCAC 
CGCTACAGGC CAGGGACCGT GGCTCTGAGA GAGATCCGTC GTTACCAGAA ATCGACTGAG 
CTGCTCATCC GGAAGCTGCC ATTCCAGAGA TTGGTGAGGG AGATCGCCCA GGATTTCAAA 
ACCGACTTGA GGTTTCAAAG TGCAGCCATC GGTGCCCTTC AGGAGGCTAG CGAAGCATAC 
CTGGTGGGGT TGTTTGAAGA TACCAATCTG TGTGCCATCC ACGCCAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGGG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H3f3b ( XM_006532248.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532311.1 Mus musculus H3.3 histone B (H3f3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22067
Clone ID Related Accession (Same CDS sequence)	XM_006532248.2 , XM_006532248.3 , NM_008211.3
Accession Version	XM_006532248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histone H3.3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532248.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGAA CCAAGCAGAC CGCTAGGAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACCA AGGCGGCTCG GAAAAGCGCG CCCTCTACCG GCGGGGTGAA GAAGCCTCAC 
CGCTACAGGC CAGGGACCGT GGCTCTGAGA GAGATCCGTC GTTACCAGAA ATCGACTGAG 
CTGCTCATCC GGAAGCTGCC ATTCCAGAGA TTGGTGAGGG AGATCGCCCA GGATTTCAAA 
ACCGACTTGA GGTTTCAAAG TGCAGCCATC GGTGCCCTTC AGGAGGCTAG CGAAGCATAC 
CTGGTGGGGT TGTTTGAAGA TACCAATCTG TGTGCCATCC ACGCCAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGGG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532311.1
CDS	1086..1496
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	XM_006532248.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus H3.3 histone B (H3f3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532248.3

ORF Insert Sequence:

ATGGCCCGAA CCAAGCAGAC CGCTAGGAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACCA AGGCGGCTCG GAAAAGCGCG CCCTCTACCG GCGGGGTGAA GAAGCCTCAC 
CGCTACAGGC CAGGGACCGT GGCTCTGAGA GAGATCCGTC GTTACCAGAA ATCGACTGAG 
CTGCTCATCC GGAAGCTGCC ATTCCAGAGA TTGGTGAGGG AGATCGCCCA GGATTTCAAA 
ACCGACTTGA GGTTTCAAAG TGCAGCCATC GGTGCCCTTC AGGAGGCTAG CGAAGCATAC 
CTGGTGGGGT TGTTTGAAGA TACCAATCTG TGTGCCATCC ACGCCAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGGG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos.
The Journal of biological chemistry(2018 Jan)
Kong Q,Banaszynski LA,Geng F,Zhang X,Zhang J,Zhang H,O'Neill CL,Yan P,Liu Z,Shido K,Palermo GD,Allis CD,Rafii S,Rosenwaks Z,Wen D

Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain.
Cell death and differentiation24(9)1548-1563(2017 Sep)
Xia W,Jiao J

Aberrant H3.3 dynamics in NAc promote vulnerability to depressive-like behavior.
Proceedings of the National Academy of Sciences of the United States of America113(44)12562-12567(2016 Nov)
Lepack AE,Bagot RC,Pe?a CJ,Loh YE,Farrelly LA,Lu Y,Powell SK,Lorsch ZS,Issler O,Cates HM,Tamminga CA,Molina H,Shen L,Nestler EJ,Allis CD,Maze I

Histone variant H3.3 provides the heterochromatic H3 lysine 9 tri-methylation mark at telomeres.
Nucleic acids research43(21)10227-37(2015 Dec)
Udugama M,M Chang FT,Chan FL,Tang MC,Pickett HA,R McGhie JD,Mayne L,Collas P,Mann JR,Wong LH

Tissue-specific expression of histone H3 variants diversified after species separation.
Epigenetics & chromatin835(2015)
Maehara K,Harada A,Sato Y,Matsumoto M,Nakayama KI,Kimura H,Ohkawa Y

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H3f3b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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