H3f3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H3f3a
Entrez Gene ID	15078
Full Name	H3 histone, family 3A
Synonyms	EyeLinc14,H3.3A
General protein information	Preferred Names H3 histone, family 3A Names histone H3.3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H4
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008210.4	NP_032236.1	histone H3.3
NM_008210.5	NP_032236.1	histone H3.3

Pathways from BioSystems

Homologies

Homo sapiens (human)	H3F3B	NP_005315.1
Mus musculus (house mouse)	H3f3b	NP_032237.1
Gallus gallus (chicken)	H3F3B	NP_990627.1
Pan troglodytes (chimpanzee)	H3F3B	XP_003315784.1
Pan troglodytes (chimpanzee)	H3F3A	XP_003339055.1
	LOC707040	XP_001105140.1
Rattus norvegicus (Norway rat)	H3f3b	NP_446437.1
Drosophila melanogaster (fruit fly)	His3:CG33857	NP_001027373.1
Homo sapiens (human)	H3F3A	NP_002098.1
Mus musculus (house mouse)	H3f3a	NP_032236.1
Gallus gallus (chicken)	LOC100859629	XP_003643522.1
Xenopus tropicalis (tropical clawed frog)	LOC101730642	XP_004913911.1
Canis lupus familiaris (dog)	H3F3B	XP_537232.1
Bos taurus (cattle)	H3F3A	NP_001014411.1
Bos taurus (cattle)	H3F3B	NP_001229500.1
Pan troglodytes (chimpanzee)	HIST1H3A	NP_001092038.1
Rattus norvegicus (Norway rat)	LOC100361558	XP_002728089.1
Danio rerio (zebrafish)	LOC565028	XP_693431.3

Gene Ontology
Bibliography

Related articles in PubMed

Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos.
Kong Q, Banaszynski LA, Geng F, Zhang X, Zhang J, Zhang H, O'Neill CL, Yan P, Liu Z, Shido K, Palermo GD, Allis CD, Rafii S, Rosenwaks Z, Wen D
The Journal of biological chemistry(2018 Jan)

Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior.
Jung EM, Moffat JJ, Liu J, Dravid SM, Gurumurthy CB, Kim WY
Nature neuroscience20(12)1694-1707(2017 Dec)

Zfp296 negatively regulates H3K9 methylation in embryonic development as a component of heterochromatin.
Matsuura T, Miyazaki S, Miyazaki T, Tashiro F, Miyazaki JI
Scientific reports7(1)12462(2017 Sep)

Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain.
Xia W, Jiao J
Cell death and differentiation24(9)1548-1563(2017 Sep)

Muscle-relevant genes marked by stable H3K4me2/3 profiles and enriched MyoD binding during myogenic differentiation.
Cui H, Bansal V, Grunert M, Malecova B, Dall'Agnese A, Latella L, Gatto S, Ryan T, Schulz K, Chen W, Dorn C, Puri PL, Sperling SR
PloS one12(6)e0179464(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results point to the importance of integrating histone modifications and MyoD chromatin binding for coordinated gene activation and repression during myogenic differentiation.
Title: Muscle-relevant genes marked by stable H3K4me2/3 profiles and enriched MyoD binding during myogenic differentiation.

In H3f3a and H3f3b null mouse embryonic stem cells, H3.3 deficiency results in reduced levels of H3K9me3 level, H4K20me3 and ATRX at the telomeres, accompanied with an increase in telomeric transcription.
Title: Histone variant H3.3 provides the heterochromatic H3 lysine 9 tri-methylation mark at telomeres.

Data show that circulating histone H3 increased significantly in necrotizing pancreatitis.
Title: Circulating Histone Levels Reflect Disease Severity in Animal Models of Acute Pancreatitis.

an important function of H3.3 is to support chromosomal heterochromatic structures, thus maintaining genome integrity during mammalian development.
Title: Histone H3.3 maintains genome integrity during mammalian development.

There is a decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.
Title: Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.

The following H3f3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H3f3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23022	NM_008210.5 Latest version!	Mus musculus H3.3 histone A (H3f3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu23022	NM_008210.4	Mus musculus H3 histone, family 3A (H3f3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H3f3a ( NM_008210.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032236.1 Mus musculus H3 histone, family 3A (H3f3a), mRNA.

Price & Availability↑

CloneID	OMu23022
Clone ID Related Accession (Same CDS sequence)	NM_008210.4 , NM_008210.5
Accession Version	NM_008210.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	histone H3.3
Comment

ATGGCTCGTA CAAAGCAGAC TGCCCGCAAA TCCACCGGTG GTAAAGCACC CAGGAAACAA 
CTGGCTACAA AAGCCGCTCG CAAGAGTGCG CCCTCTACTG GAGGGGTGAA GAAACCTCAT 
CGTTACAGGC CTGGTACTGT GGCCCTCCGT GAAATCAGAC GCTATCAGAA GTCCACTGAA 
CTTCTGATCC GCAAGCTCCC CTTTCAGCGT CTGGTGCGAG AAATTGCTCA GGACTTCAAA 
ACAGATCTGC GCTTCCAGAG TGCAGCTATT GGTGCTTTGC AGGAGGCAAG TGAGGCCTAT 
CTGGTTGGCC TTTTTGAAGA TACCAATCTG TGTGCTATCC ATGCCAAACG TGTAACAATT 
ATGCCAAAAG ATATCCAGCT AGCACGCCGC ATACGCGGAG AACGTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032236.1
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_008210.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus H3 histone, family 3A (H3f3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008210.4

ORF Insert Sequence:

ATGGCTCGTA CAAAGCAGAC TGCCCGCAAA TCCACCGGTG GTAAAGCACC CAGGAAACAA 
CTGGCTACAA AAGCCGCTCG CAAGAGTGCG CCCTCTACTG GAGGGGTGAA GAAACCTCAT 
CGTTACAGGC CTGGTACTGT GGCCCTCCGT GAAATCAGAC GCTATCAGAA GTCCACTGAA 
CTTCTGATCC GCAAGCTCCC CTTTCAGCGT CTGGTGCGAG AAATTGCTCA GGACTTCAAA 
ACAGATCTGC GCTTCCAGAG TGCAGCTATT GGTGCTTTGC AGGAGGCAAG TGAGGCCTAT 
CTGGTTGGCC TTTTTGAAGA TACCAATCTG TGTGCTATCC ATGCCAAACG TGTAACAATT 
ATGCCAAAAG ATATCCAGCT AGCACGCCGC ATACGCGGAG AACGTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H3f3a ( NM_008210.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032236.1 Mus musculus H3.3 histone A (H3f3a), mRNA.

Price & Availability↑

CloneID	OMu23022
Clone ID Related Accession (Same CDS sequence)	NM_008210.4 , NM_008210.5
Accession Version	NM_008210.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	histone H3.3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC121292.9. On Nov 5, 2015 this sequence version replaced NM_008210.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA465622.1, AK037900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 9373943 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGTA CAAAGCAGAC TGCCCGCAAA TCCACCGGTG GTAAAGCACC CAGGAAACAA 
CTGGCTACAA AAGCCGCTCG CAAGAGTGCG CCCTCTACTG GAGGGGTGAA GAAACCTCAT 
CGTTACAGGC CTGGTACTGT GGCCCTCCGT GAAATCAGAC GCTATCAGAA GTCCACTGAA 
CTTCTGATCC GCAAGCTCCC CTTTCAGCGT CTGGTGCGAG AAATTGCTCA GGACTTCAAA 
ACAGATCTGC GCTTCCAGAG TGCAGCTATT GGTGCTTTGC AGGAGGCAAG TGAGGCCTAT 
CTGGTTGGCC TTTTTGAAGA TACCAATCTG TGTGCTATCC ATGCCAAACG TGTAACAATT 
ATGCCAAAAG ATATCCAGCT AGCACGCCGC ATACGCGGAG AACGTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032236.1
CDS	159..569
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_008210.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus H3.3 histone A (H3f3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008210.5

ORF Insert Sequence:

ATGGCTCGTA CAAAGCAGAC TGCCCGCAAA TCCACCGGTG GTAAAGCACC CAGGAAACAA 
CTGGCTACAA AAGCCGCTCG CAAGAGTGCG CCCTCTACTG GAGGGGTGAA GAAACCTCAT 
CGTTACAGGC CTGGTACTGT GGCCCTCCGT GAAATCAGAC GCTATCAGAA GTCCACTGAA 
CTTCTGATCC GCAAGCTCCC CTTTCAGCGT CTGGTGCGAG AAATTGCTCA GGACTTCAAA 
ACAGATCTGC GCTTCCAGAG TGCAGCTATT GGTGCTTTGC AGGAGGCAAG TGAGGCCTAT 
CTGGTTGGCC TTTTTGAAGA TACCAATCTG TGTGCTATCC ATGCCAAACG TGTAACAATT 
ATGCCAAAAG ATATCCAGCT AGCACGCCGC ATACGCGGAG AACGTGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos.
The Journal of biological chemistry(2018 Jan)
Kong Q,Banaszynski LA,Geng F,Zhang X,Zhang J,Zhang H,O'Neill CL,Yan P,Liu Z,Shido K,Palermo GD,Allis CD,Rafii S,Rosenwaks Z,Wen D

Arid1b haploinsufficiency disrupts cortical interneuron development and mouse behavior.
Nature neuroscience20(12)1694-1707(2017 Dec)
Jung EM,Moffat JJ,Liu J,Dravid SM,Gurumurthy CB,Kim WY

Zfp296 negatively regulates H3K9 methylation in embryonic development as a component of heterochromatin.
Scientific reports7(1)12462(2017 Sep)
Matsuura T,Miyazaki S,Miyazaki T,Tashiro F,Miyazaki JI

Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain.
Cell death and differentiation24(9)1548-1563(2017 Sep)
Xia W,Jiao J

Muscle-relevant genes marked by stable H3K4me2/3 profiles and enriched MyoD binding during myogenic differentiation.
PloS one12(6)e0179464(2017)
Cui H,Bansal V,Grunert M,Malecova B,Dall'Agnese A,Latella L,Gatto S,Ryan T,Schulz K,Chen W,Dorn C,Puri PL,Sperling SR

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H3f3a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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