Grb7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grb7
Entrez Gene ID	14786
Full Name	growth factor receptor bound protein 7
Synonyms	mKIAA4028
General protein information	Preferred Names growth factor receptor bound protein 7 Names growth factor receptor-bound protein 7 GRB7 adapter protein Growth factor receptor-bound protein 7 (GRB7 adapter protein) (Epidermal growth factor receptor GRB-7) epidermal growth factor receptor GRB-7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 D\|11 61.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532233.1	XP_006532296.1	growth factor receptor-bound protein 7 isoform X1
NM_010346.2	NP_034476.1	growth factor receptor-bound protein 7
XM_030245575.1	XP_030101435.1	growth factor receptor-bound protein 7 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRB7	XP_001171807.2
Gallus gallus (chicken)	GRB7	XP_004948652.1
Homo sapiens (human)	GRB7	NP_001229371.1
Rattus norvegicus (Norway rat)	Grb7	NP_445855.1
Xenopus tropicalis (tropical clawed frog)	grb7	XP_004918734.1
	GRB7	XP_001091728.1
Mus musculus (house mouse)	Grb7	NP_034476.1
Bos taurus (cattle)	GRB7	NP_001039479.1
Canis lupus familiaris (dog)	GRB7	XP_548141.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Mmu-miR-193 is involved in embryo implantation in mouse uterus by regulating GRB7 gene expression.
Li R, He J, Chen X, Ding Y, Wang Y, Long C, Shen L, Liu X
Reproductive sciences (Thousand Oaks, Calif.)21(6)733-42(2014 Jun)

EGF-induced Grb7 recruits and promotes Ras activity essential for the tumorigenicity of Sk-Br3 breast cancer cells.
Chu PY, Li TK, Ding ST, Lai IR, Shen TL
The Journal of biological chemistry285(38)29279-85(2010 Sep)

Kappa opioid receptor contributes to EGF-stimulated neurite extension in development.
Tsai NP, Tsui YC, Pintar JE, Loh HH, Wei LN
Proceedings of the National Academy of Sciences of the United States of America107(7)3216-21(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GRB7 gene expression in uterus during embryo implantation is regulated by Mmu-miR-193.
Title: Mmu-miR-193 is involved in embryo implantation in mouse uterus by regulating GRB7 gene expression.

Findings illustrate an underlying mechanism by which Grb7 promotes tumorigenesis through the formation of a novel EGFR-Grb7-Ras signaling complex, thereby highlighting the potential strategy of targeting Grb7 as an anti-breast cancer therapy.
Title: EGF-induced Grb7 recruits and promotes Ras activity essential for the tumorigenicity of Sk-Br3 breast cancer cells.

Grb7 is identified as an integral component of stress granules.
Title: Regulation of stress granule dynamics by Grb7 and FAK signalling pathway.

The adaptor Grb7 links netrin-1 signaling to regulation of mRNA translation of kappa opioid receptor.
Title: The adaptor Grb7 links netrin-1 signaling to regulation of mRNA translation.

The following Grb7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grb7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05999	NM_010346.2 Latest version!	Mus musculus growth factor receptor bound protein 7 (Grb7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05999	XM_006532233.1 Latest version!	Mus musculus growth factor receptor bound protein 7 (Grb7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05999	XM_030245575.1 Latest version!	Mus musculus growth factor receptor bound protein 7 (Grb7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grb7 ( NM_010346.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034476.1 Mus musculus growth factor receptor bound protein 7 (Grb7), mRNA.

Price & Availability↑

CloneID	OMu05999
Clone ID Related Accession (Same CDS sequence)	XM_006532233.1 , NM_010346.2 , XM_030245575.1
Accession Version	NM_010346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	growth factor receptor-bound protein 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA496861.1, AK168438.1 and AW494536.1. On Oct 21, 2006 this sequence version replaced NM_010346.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M94450.1, AK057640.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTGG ATCTGAGCCC GACTCATCTC AGCAGCTCCC CAGAAGATGT GTGCCCAACT 
CCTGCTACCC CTCCTGAGAC TCCTCCGCCC CCTGATAACC CTCCGCCAGG GGATGTGAAG 
CGGTCGCAGC CTTTGCCCAT CCCCAGCAGC AGGAAACTTC GAGAAGAGGA GTTTCAGGCA 
ACCTCTCTGC CTTCCATCCC CAACCCCTTC CCTGAGCTCT GCAGCCCACC TTCACAGAAA 
CCCATTCTTG GTGGTTCCTC CGGTGCAAGG GGGTTGCTTC CTCGAGACTC CAGCCGCCTC 
TGTGTGGTGA AGGTGTACAG TGAGGATGGG GCCTGCCGGT CTGTGGAGGT GGCAGCGGGC 
GCCACAGCTC GTCACGTGTG TGAGATGCTG GTACAGCGAG CTCACGCCCT GAGCGACGAG 
AGCTGGGGAC TAGTGGAATC CCACCCCTAC CTGGCACTGG AGCGGGGTCT GGAGGACCAT 
GAATTTGTGG TGGAAGTGCA GGAGGCCTGG CCTGTGGGTG GAGACAGCCG CTTCATCTTT 
CGTAAAAACT TCGCCAAGTA TGAACTATTC AAGAGCCCCC CACACACCCT GTTCCCAGAA 
AAGATGGTCT CGAGCTGTCT GGATGCACAA ACAGGCATAT CCCATGAAGA CCTCATCCAG 
AACTTCCTGA ACGCTGGCAG CTTCCCTGAG ATCCAGGGCT TCCTGCAGCT GCGGGGATCA 
GGCCGGGGGT CAGGTCGAAA GCTTTGGAAA CGTTTCTTCT GCTTTCTGCG TCGATCTGGC 
CTCTACTACT CTACCAAGGG TACCTCCAAG GACCCCAGAC ACCTACAGTA TGTGGCAGAT 
GTGAATGAGT CCAATGTCTA TGTGGTGACC CAGGGCCGCA AGCTGTATGG GATGCCCACT 
GACTTCGGCT TCTGTGTCAA GCCCAACAAG CTTCGAAACG GCCACAAGGG GCTCCACATC 
TTCTGCAGTG AGGATGAGCA GAGTCGGACC TGCTGGCTGG CTGCCTTCCG GCTCTTCAAG 
TACGGGGTAC AGCTATATAA GAATTATCAG CAGGCCCAGT CTCGTCACCT GCGCCTATCC 
TATTTGGGGT CTCCACCCTT GAGGAGCGTC TCAGACAATA CCCTAGTGGC TATGGACTTC 
TCTGGCCATG CGGGGCGTGT CATTGATAAC CCCCGGGAAG CTCTGAGTGC CGCCATGGAG 
GAGGCCCAGG CCTGGAGGAA GAAGACAAAC CACCGTCTGA GCCTGCCCAC CACATGCTCT 
GGCTCGAGCC TCAGCGCAGC CATTCATCGC ACCCAGCCCT GGTTTCATGG ACGCATCTCT 
CGGGAGGAGA GCCAGCGGCT AATTGGACAG CAGGGCCTGG TGGATGGTGT GTTCCTGGTC 
CGGGAGAGCC AGAGGAACCC ACAGGGCTTT GTCCTGTCCT TGTGCCATCT GCAGAAAGTC 
AAGCATTATC TCATTTTGCC AAGCGAAGAT GAAGGTTGCC TTTACTTCAG CATGGATGAG 
GGCCAGACCC GTTTCACAGA CCTGCTGCAG CTGGTAGAAT TCCACCAGCT GAACCGAGGC 
ATCCTGCCCT GCCTGCTGCG CCACTGCTGT GCCCGTGTGG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034476.1
CDS	422..2029
Translation	MELDLSPTHLSSSPEDVCPTPATPPETPPPPDNPPPGDVKRSQPLPIPSSRKLREEEFQATSLPSIPNPFPELCSPPSQKPILGGSSGARGLLPRDSSRLCVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDESWGLVESHPYLALERGLEDHEFVVEVQEAWPVGGDSRFIFRKNFAKYELFKSPPHTLFPEKMVSSCLDAQTGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLHIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLRLSYLGSPPLRSVSDNTLVAMDFSGHAGRVIDNPREALSAAMEEAQAWRKKTNHRLSLPTTCSGSSLSAAIHRTQPWFHGRISREESQRLIGQQGLVDGVFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEDEGCLYFSMDEGQTRFTDLLQLVEFHQLNRGILPCLLRHCCARVAL

Target ORF information:

RefSeq Version	NM_010346.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth factor receptor bound protein 7 (Grb7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010346.2

ORF Insert Sequence:

ATGGAACTGG ATCTGAGCCC GACTCATCTC AGCAGCTCCC CAGAAGATGT GTGCCCAACT 
CCTGCTACCC CTCCTGAGAC TCCTCCGCCC CCTGATAACC CTCCGCCAGG GGATGTGAAG 
CGGTCGCAGC CTTTGCCCAT CCCCAGCAGC AGGAAACTTC GAGAAGAGGA GTTTCAGGCA 
ACCTCTCTGC CTTCCATCCC CAACCCCTTC CCTGAGCTCT GCAGCCCACC TTCACAGAAA 
CCCATTCTTG GTGGTTCCTC CGGTGCAAGG GGGTTGCTTC CTCGAGACTC CAGCCGCCTC 
TGTGTGGTGA AGGTGTACAG TGAGGATGGG GCCTGCCGGT CTGTGGAGGT GGCAGCGGGC 
GCCACAGCTC GTCACGTGTG TGAGATGCTG GTACAGCGAG CTCACGCCCT GAGCGACGAG 
AGCTGGGGAC TAGTGGAATC CCACCCCTAC CTGGCACTGG AGCGGGGTCT GGAGGACCAT 
GAATTTGTGG TGGAAGTGCA GGAGGCCTGG CCTGTGGGTG GAGACAGCCG CTTCATCTTT 
CGTAAAAACT TCGCCAAGTA TGAACTATTC AAGAGCCCCC CACACACCCT GTTCCCAGAA 
AAGATGGTCT CGAGCTGTCT GGATGCACAA ACAGGCATAT CCCATGAAGA CCTCATCCAG 
AACTTCCTGA ACGCTGGCAG CTTCCCTGAG ATCCAGGGCT TCCTGCAGCT GCGGGGATCA 
GGCCGGGGGT CAGGTCGAAA GCTTTGGAAA CGTTTCTTCT GCTTTCTGCG TCGATCTGGC 
CTCTACTACT CTACCAAGGG TACCTCCAAG GACCCCAGAC ACCTACAGTA TGTGGCAGAT 
GTGAATGAGT CCAATGTCTA TGTGGTGACC CAGGGCCGCA AGCTGTATGG GATGCCCACT 
GACTTCGGCT TCTGTGTCAA GCCCAACAAG CTTCGAAACG GCCACAAGGG GCTCCACATC 
TTCTGCAGTG AGGATGAGCA GAGTCGGACC TGCTGGCTGG CTGCCTTCCG GCTCTTCAAG 
TACGGGGTAC AGCTATATAA GAATTATCAG CAGGCCCAGT CTCGTCACCT GCGCCTATCC 
TATTTGGGGT CTCCACCCTT GAGGAGCGTC TCAGACAATA CCCTAGTGGC TATGGACTTC 
TCTGGCCATG CGGGGCGTGT CATTGATAAC CCCCGGGAAG CTCTGAGTGC CGCCATGGAG 
GAGGCCCAGG CCTGGAGGAA GAAGACAAAC CACCGTCTGA GCCTGCCCAC CACATGCTCT 
GGCTCGAGCC TCAGCGCAGC CATTCATCGC ACCCAGCCCT GGTTTCATGG ACGCATCTCT 
CGGGAGGAGA GCCAGCGGCT AATTGGACAG CAGGGCCTGG TGGATGGTGT GTTCCTGGTC 
CGGGAGAGCC AGAGGAACCC ACAGGGCTTT GTCCTGTCCT TGTGCCATCT GCAGAAAGTC 
AAGCATTATC TCATTTTGCC AAGCGAAGAT GAAGGTTGCC TTTACTTCAG CATGGATGAG 
GGCCAGACCC GTTTCACAGA CCTGCTGCAG CTGGTAGAAT TCCACCAGCT GAACCGAGGC 
ATCCTGCCCT GCCTGCTGCG CCACTGCTGT GCCCGTGTGG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grb7 ( XM_006532233.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532296.1 Mus musculus growth factor receptor bound protein 7 (Grb7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu05999
Clone ID Related Accession (Same CDS sequence)	XM_006532233.1 , NM_010346.2 , XM_030245575.1
Accession Version	XM_006532233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	growth factor receptor-bound protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG ATCTGAGCCC GACTCATCTC AGCAGCTCCC CAGAAGATGT GTGCCCAACT 
CCTGCTACCC CTCCTGAGAC TCCTCCGCCC CCTGATAACC CTCCGCCAGG GGATGTGAAG 
CGGTCGCAGC CTTTGCCCAT CCCCAGCAGC AGGAAACTTC GAGAAGAGGA GTTTCAGGCA 
ACCTCTCTGC CTTCCATCCC CAACCCCTTC CCTGAGCTCT GCAGCCCACC TTCACAGAAA 
CCCATTCTTG GTGGTTCCTC CGGTGCAAGG GGGTTGCTTC CTCGAGACTC CAGCCGCCTC 
TGTGTGGTGA AGGTGTACAG TGAGGATGGG GCCTGCCGGT CTGTGGAGGT GGCAGCGGGC 
GCCACAGCTC GTCACGTGTG TGAGATGCTG GTACAGCGAG CTCACGCCCT GAGCGACGAG 
AGCTGGGGAC TAGTGGAATC CCACCCCTAC CTGGCACTGG AGCGGGGTCT GGAGGACCAT 
GAATTTGTGG TGGAAGTGCA GGAGGCCTGG CCTGTGGGTG GAGACAGCCG CTTCATCTTT 
CGTAAAAACT TCGCCAAGTA TGAACTATTC AAGAGCCCCC CACACACCCT GTTCCCAGAA 
AAGATGGTCT CGAGCTGTCT GGATGCACAA ACAGGCATAT CCCATGAAGA CCTCATCCAG 
AACTTCCTGA ACGCTGGCAG CTTCCCTGAG ATCCAGGGCT TCCTGCAGCT GCGGGGATCA 
GGCCGGGGGT CAGGTCGAAA GCTTTGGAAA CGTTTCTTCT GCTTTCTGCG TCGATCTGGC 
CTCTACTACT CTACCAAGGG TACCTCCAAG GACCCCAGAC ACCTACAGTA TGTGGCAGAT 
GTGAATGAGT CCAATGTCTA TGTGGTGACC CAGGGCCGCA AGCTGTATGG GATGCCCACT 
GACTTCGGCT TCTGTGTCAA GCCCAACAAG CTTCGAAACG GCCACAAGGG GCTCCACATC 
TTCTGCAGTG AGGATGAGCA GAGTCGGACC TGCTGGCTGG CTGCCTTCCG GCTCTTCAAG 
TACGGGGTAC AGCTATATAA GAATTATCAG CAGGCCCAGT CTCGTCACCT GCGCCTATCC 
TATTTGGGGT CTCCACCCTT GAGGAGCGTC TCAGACAATA CCCTAGTGGC TATGGACTTC 
TCTGGCCATG CGGGGCGTGT CATTGATAAC CCCCGGGAAG CTCTGAGTGC CGCCATGGAG 
GAGGCCCAGG CCTGGAGGAA GAAGACAAAC CACCGTCTGA GCCTGCCCAC CACATGCTCT 
GGCTCGAGCC TCAGCGCAGC CATTCATCGC ACCCAGCCCT GGTTTCATGG ACGCATCTCT 
CGGGAGGAGA GCCAGCGGCT AATTGGACAG CAGGGCCTGG TGGATGGTGT GTTCCTGGTC 
CGGGAGAGCC AGAGGAACCC ACAGGGCTTT GTCCTGTCCT TGTGCCATCT GCAGAAAGTC 
AAGCATTATC TCATTTTGCC AAGCGAAGAT GAAGGTTGCC TTTACTTCAG CATGGATGAG 
GGCCAGACCC GTTTCACAGA CCTGCTGCAG CTGGTAGAAT TCCACCAGCT GAACCGAGGC 
ATCCTGCCCT GCCTGCTGCG CCACTGCTGT GCCCGTGTGG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532296.1
CDS	30..1637
Translation	MELDLSPTHLSSSPEDVCPTPATPPETPPPPDNPPPGDVKRSQPLPIPSSRKLREEEFQATSLPSIPNPFPELCSPPSQKPILGGSSGARGLLPRDSSRLCVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDESWGLVESHPYLALERGLEDHEFVVEVQEAWPVGGDSRFIFRKNFAKYELFKSPPHTLFPEKMVSSCLDAQTGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLHIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLRLSYLGSPPLRSVSDNTLVAMDFSGHAGRVIDNPREALSAAMEEAQAWRKKTNHRLSLPTTCSGSSLSAAIHRTQPWFHGRISREESQRLIGQQGLVDGVFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEDEGCLYFSMDEGQTRFTDLLQLVEFHQLNRGILPCLLRHCCARVAL

Target ORF information:

RefSeq Version	XM_006532233.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth factor receptor bound protein 7 (Grb7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532233.1

ORF Insert Sequence:

ATGGAACTGG ATCTGAGCCC GACTCATCTC AGCAGCTCCC CAGAAGATGT GTGCCCAACT 
CCTGCTACCC CTCCTGAGAC TCCTCCGCCC CCTGATAACC CTCCGCCAGG GGATGTGAAG 
CGGTCGCAGC CTTTGCCCAT CCCCAGCAGC AGGAAACTTC GAGAAGAGGA GTTTCAGGCA 
ACCTCTCTGC CTTCCATCCC CAACCCCTTC CCTGAGCTCT GCAGCCCACC TTCACAGAAA 
CCCATTCTTG GTGGTTCCTC CGGTGCAAGG GGGTTGCTTC CTCGAGACTC CAGCCGCCTC 
TGTGTGGTGA AGGTGTACAG TGAGGATGGG GCCTGCCGGT CTGTGGAGGT GGCAGCGGGC 
GCCACAGCTC GTCACGTGTG TGAGATGCTG GTACAGCGAG CTCACGCCCT GAGCGACGAG 
AGCTGGGGAC TAGTGGAATC CCACCCCTAC CTGGCACTGG AGCGGGGTCT GGAGGACCAT 
GAATTTGTGG TGGAAGTGCA GGAGGCCTGG CCTGTGGGTG GAGACAGCCG CTTCATCTTT 
CGTAAAAACT TCGCCAAGTA TGAACTATTC AAGAGCCCCC CACACACCCT GTTCCCAGAA 
AAGATGGTCT CGAGCTGTCT GGATGCACAA ACAGGCATAT CCCATGAAGA CCTCATCCAG 
AACTTCCTGA ACGCTGGCAG CTTCCCTGAG ATCCAGGGCT TCCTGCAGCT GCGGGGATCA 
GGCCGGGGGT CAGGTCGAAA GCTTTGGAAA CGTTTCTTCT GCTTTCTGCG TCGATCTGGC 
CTCTACTACT CTACCAAGGG TACCTCCAAG GACCCCAGAC ACCTACAGTA TGTGGCAGAT 
GTGAATGAGT CCAATGTCTA TGTGGTGACC CAGGGCCGCA AGCTGTATGG GATGCCCACT 
GACTTCGGCT TCTGTGTCAA GCCCAACAAG CTTCGAAACG GCCACAAGGG GCTCCACATC 
TTCTGCAGTG AGGATGAGCA GAGTCGGACC TGCTGGCTGG CTGCCTTCCG GCTCTTCAAG 
TACGGGGTAC AGCTATATAA GAATTATCAG CAGGCCCAGT CTCGTCACCT GCGCCTATCC 
TATTTGGGGT CTCCACCCTT GAGGAGCGTC TCAGACAATA CCCTAGTGGC TATGGACTTC 
TCTGGCCATG CGGGGCGTGT CATTGATAAC CCCCGGGAAG CTCTGAGTGC CGCCATGGAG 
GAGGCCCAGG CCTGGAGGAA GAAGACAAAC CACCGTCTGA GCCTGCCCAC CACATGCTCT 
GGCTCGAGCC TCAGCGCAGC CATTCATCGC ACCCAGCCCT GGTTTCATGG ACGCATCTCT 
CGGGAGGAGA GCCAGCGGCT AATTGGACAG CAGGGCCTGG TGGATGGTGT GTTCCTGGTC 
CGGGAGAGCC AGAGGAACCC ACAGGGCTTT GTCCTGTCCT TGTGCCATCT GCAGAAAGTC 
AAGCATTATC TCATTTTGCC AAGCGAAGAT GAAGGTTGCC TTTACTTCAG CATGGATGAG 
GGCCAGACCC GTTTCACAGA CCTGCTGCAG CTGGTAGAAT TCCACCAGCT GAACCGAGGC 
ATCCTGCCCT GCCTGCTGCG CCACTGCTGT GCCCGTGTGG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grb7 ( XM_030245575.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101435.1 Mus musculus growth factor receptor bound protein 7 (Grb7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05999
Clone ID Related Accession (Same CDS sequence)	XM_006532233.1 , NM_010346.2 , XM_030245575.1
Accession Version	XM_030245575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	growth factor receptor-bound protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG ATCTGAGCCC GACTCATCTC AGCAGCTCCC CAGAAGATGT GTGCCCAACT 
CCTGCTACCC CTCCTGAGAC TCCTCCGCCC CCTGATAACC CTCCGCCAGG GGATGTGAAG 
CGGTCGCAGC CTTTGCCCAT CCCCAGCAGC AGGAAACTTC GAGAAGAGGA GTTTCAGGCA 
ACCTCTCTGC CTTCCATCCC CAACCCCTTC CCTGAGCTCT GCAGCCCACC TTCACAGAAA 
CCCATTCTTG GTGGTTCCTC CGGTGCAAGG GGGTTGCTTC CTCGAGACTC CAGCCGCCTC 
TGTGTGGTGA AGGTGTACAG TGAGGATGGG GCCTGCCGGT CTGTGGAGGT GGCAGCGGGC 
GCCACAGCTC GTCACGTGTG TGAGATGCTG GTACAGCGAG CTCACGCCCT GAGCGACGAG 
AGCTGGGGAC TAGTGGAATC CCACCCCTAC CTGGCACTGG AGCGGGGTCT GGAGGACCAT 
GAATTTGTGG TGGAAGTGCA GGAGGCCTGG CCTGTGGGTG GAGACAGCCG CTTCATCTTT 
CGTAAAAACT TCGCCAAGTA TGAACTATTC AAGAGCCCCC CACACACCCT GTTCCCAGAA 
AAGATGGTCT CGAGCTGTCT GGATGCACAA ACAGGCATAT CCCATGAAGA CCTCATCCAG 
AACTTCCTGA ACGCTGGCAG CTTCCCTGAG ATCCAGGGCT TCCTGCAGCT GCGGGGATCA 
GGCCGGGGGT CAGGTCGAAA GCTTTGGAAA CGTTTCTTCT GCTTTCTGCG TCGATCTGGC 
CTCTACTACT CTACCAAGGG TACCTCCAAG GACCCCAGAC ACCTACAGTA TGTGGCAGAT 
GTGAATGAGT CCAATGTCTA TGTGGTGACC CAGGGCCGCA AGCTGTATGG GATGCCCACT 
GACTTCGGCT TCTGTGTCAA GCCCAACAAG CTTCGAAACG GCCACAAGGG GCTCCACATC 
TTCTGCAGTG AGGATGAGCA GAGTCGGACC TGCTGGCTGG CTGCCTTCCG GCTCTTCAAG 
TACGGGGTAC AGCTATATAA GAATTATCAG CAGGCCCAGT CTCGTCACCT GCGCCTATCC 
TATTTGGGGT CTCCACCCTT GAGGAGCGTC TCAGACAATA CCCTAGTGGC TATGGACTTC 
TCTGGCCATG CGGGGCGTGT CATTGATAAC CCCCGGGAAG CTCTGAGTGC CGCCATGGAG 
GAGGCCCAGG CCTGGAGGAA GAAGACAAAC CACCGTCTGA GCCTGCCCAC CACATGCTCT 
GGCTCGAGCC TCAGCGCAGC CATTCATCGC ACCCAGCCCT GGTTTCATGG ACGCATCTCT 
CGGGAGGAGA GCCAGCGGCT AATTGGACAG CAGGGCCTGG TGGATGGTGT GTTCCTGGTC 
CGGGAGAGCC AGAGGAACCC ACAGGGCTTT GTCCTGTCCT TGTGCCATCT GCAGAAAGTC 
AAGCATTATC TCATTTTGCC AAGCGAAGAT GAAGGTTGCC TTTACTTCAG CATGGATGAG 
GGCCAGACCC GTTTCACAGA CCTGCTGCAG CTGGTAGAAT TCCACCAGCT GAACCGAGGC 
ATCCTGCCCT GCCTGCTGCG CCACTGCTGT GCCCGTGTGG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101435.1
CDS	1828..3435
Translation	MELDLSPTHLSSSPEDVCPTPATPPETPPPPDNPPPGDVKRSQPLPIPSSRKLREEEFQATSLPSIPNPFPELCSPPSQKPILGGSSGARGLLPRDSSRLCVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDESWGLVESHPYLALERGLEDHEFVVEVQEAWPVGGDSRFIFRKNFAKYELFKSPPHTLFPEKMVSSCLDAQTGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLHIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLRLSYLGSPPLRSVSDNTLVAMDFSGHAGRVIDNPREALSAAMEEAQAWRKKTNHRLSLPTTCSGSSLSAAIHRTQPWFHGRISREESQRLIGQQGLVDGVFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEDEGCLYFSMDEGQTRFTDLLQLVEFHQLNRGILPCLLRHCCARVAL

Target ORF information:

RefSeq Version	XM_030245575.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth factor receptor bound protein 7 (Grb7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245575.1

ORF Insert Sequence:

ATGGAACTGG ATCTGAGCCC GACTCATCTC AGCAGCTCCC CAGAAGATGT GTGCCCAACT 
CCTGCTACCC CTCCTGAGAC TCCTCCGCCC CCTGATAACC CTCCGCCAGG GGATGTGAAG 
CGGTCGCAGC CTTTGCCCAT CCCCAGCAGC AGGAAACTTC GAGAAGAGGA GTTTCAGGCA 
ACCTCTCTGC CTTCCATCCC CAACCCCTTC CCTGAGCTCT GCAGCCCACC TTCACAGAAA 
CCCATTCTTG GTGGTTCCTC CGGTGCAAGG GGGTTGCTTC CTCGAGACTC CAGCCGCCTC 
TGTGTGGTGA AGGTGTACAG TGAGGATGGG GCCTGCCGGT CTGTGGAGGT GGCAGCGGGC 
GCCACAGCTC GTCACGTGTG TGAGATGCTG GTACAGCGAG CTCACGCCCT GAGCGACGAG 
AGCTGGGGAC TAGTGGAATC CCACCCCTAC CTGGCACTGG AGCGGGGTCT GGAGGACCAT 
GAATTTGTGG TGGAAGTGCA GGAGGCCTGG CCTGTGGGTG GAGACAGCCG CTTCATCTTT 
CGTAAAAACT TCGCCAAGTA TGAACTATTC AAGAGCCCCC CACACACCCT GTTCCCAGAA 
AAGATGGTCT CGAGCTGTCT GGATGCACAA ACAGGCATAT CCCATGAAGA CCTCATCCAG 
AACTTCCTGA ACGCTGGCAG CTTCCCTGAG ATCCAGGGCT TCCTGCAGCT GCGGGGATCA 
GGCCGGGGGT CAGGTCGAAA GCTTTGGAAA CGTTTCTTCT GCTTTCTGCG TCGATCTGGC 
CTCTACTACT CTACCAAGGG TACCTCCAAG GACCCCAGAC ACCTACAGTA TGTGGCAGAT 
GTGAATGAGT CCAATGTCTA TGTGGTGACC CAGGGCCGCA AGCTGTATGG GATGCCCACT 
GACTTCGGCT TCTGTGTCAA GCCCAACAAG CTTCGAAACG GCCACAAGGG GCTCCACATC 
TTCTGCAGTG AGGATGAGCA GAGTCGGACC TGCTGGCTGG CTGCCTTCCG GCTCTTCAAG 
TACGGGGTAC AGCTATATAA GAATTATCAG CAGGCCCAGT CTCGTCACCT GCGCCTATCC 
TATTTGGGGT CTCCACCCTT GAGGAGCGTC TCAGACAATA CCCTAGTGGC TATGGACTTC 
TCTGGCCATG CGGGGCGTGT CATTGATAAC CCCCGGGAAG CTCTGAGTGC CGCCATGGAG 
GAGGCCCAGG CCTGGAGGAA GAAGACAAAC CACCGTCTGA GCCTGCCCAC CACATGCTCT 
GGCTCGAGCC TCAGCGCAGC CATTCATCGC ACCCAGCCCT GGTTTCATGG ACGCATCTCT 
CGGGAGGAGA GCCAGCGGCT AATTGGACAG CAGGGCCTGG TGGATGGTGT GTTCCTGGTC 
CGGGAGAGCC AGAGGAACCC ACAGGGCTTT GTCCTGTCCT TGTGCCATCT GCAGAAAGTC 
AAGCATTATC TCATTTTGCC AAGCGAAGAT GAAGGTTGCC TTTACTTCAG CATGGATGAG 
GGCCAGACCC GTTTCACAGA CCTGCTGCAG CTGGTAGAAT TCCACCAGCT GAACCGAGGC 
ATCCTGCCCT GCCTGCTGCG CCACTGCTGT GCCCGTGTGG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Mmu-miR-193 is involved in embryo implantation in mouse uterus by regulating GRB7 gene expression.
Reproductive sciences (Thousand Oaks, Calif.)21(6)733-42(2014 Jun)
Li R,He J,Chen X,Ding Y,Wang Y,Long C,Shen L,Liu X

EGF-induced Grb7 recruits and promotes Ras activity essential for the tumorigenicity of Sk-Br3 breast cancer cells.
The Journal of biological chemistry285(38)29279-85(2010 Sep)
Chu PY,Li TK,Ding ST,Lai IR,Shen TL

Kappa opioid receptor contributes to EGF-stimulated neurite extension in development.
Proceedings of the National Academy of Sciences of the United States of America107(7)3216-21(2010 Feb)
Tsai NP,Tsui YC,Pintar JE,Loh HH,Wei LN

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Grb7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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