Gnas cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnas
Entrez Gene ID	14683
Full Name	GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus
Synonyms	5530400H20Rik,A930027G11Rik,C130027O20Rik,GPSA,GSP,Galphas,Gnas1,Gnasxl,Gs-alpha,Gsa,Nesp,Nesp55,Nespl,Oed-Sml,Oedsml,P1,P2,P3,PHP1A,PHP1B,POH,SCG6
General protein information	Preferred Names GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus Names protein ALEX protein GNAS protein SCG6 (secretogranin VI) Gs alpha subunit adenylate cyclase-stimulating G alpha protein alternative gene product encoded by XL-exon extra large alphas protein guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas neuroendocrine secretory protein 55 secretogranin VI stimulatory G-protein alpha subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H4\|2 97.89 cM
Summary	This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077510.4	NP_001070978.1	protein GNAS isoform GNASS
NM_022000.3	NP_068840.2	protein SCG6 (secretogranin VI) isoform SCG6 precursor
NM_001077510.5	NP_001070978.1	protein GNAS isoform GNASS
NM_001310083.1	NP_001297012.1	protein GNAS isoform GNASL
XM_006498777.2	XP_006498840.1	protein GNAS isoform X3
NM_001077507.2	NP_001070975.1	protein GNAS isoform f
NM_001310085.1	NP_001297014.1	protein GNAS isoform XXLb2
XM_006498779.3	XP_006498842.1	protein SCG6 (secretogranin VI) isoform X6
XM_017315658.2	XP_017171147.1	protein GNAS isoform X5
NM_001364030.1	NP_001350959.1	protein ALEX isoform i
NM_201616.3	NP_963910.1	protein GNAS isoform GNASL
NM_201617.2	NP_963911.1	protein GNAS isoform XXLb1
XM_030247569.1	XP_030103429.1	protein GNAS isoform X6
NM_201618.2	NP_963912.1	protein ALEX isoform Alex
NM_019690.3	NP_062664.2	protein SCG6 (secretogranin VI) isoform SCG6 precursor
XM_006498778.3	XP_006498841.1	protein GNAS isoform X4
XM_006498780.1	XP_006498843.1	protein GNAS isoform X8
XM_006498774.1	XP_006498837.1	protein GNAS isoform X1
XM_006498778.2	XP_006498841.1	protein GNAS isoform X4
XM_017315658.1	XP_017171147.1	protein GNAS isoform X5
XM_006498776.1	XP_006498839.1	protein GNAS isoform X2
NM_201616.2	NP_963910.1	protein GNAS isoform GNASL
XM_006498774.2	XP_006498837.1	protein GNAS isoform X1
XM_030247568.1	XP_030103428.1	protein GNAS isoform X2
XM_006498779.4	XP_006498842.1	protein SCG6 (secretogranin VI) isoform X7
NM_010309.4	NP_034439.2	protein GNAS isoform XLas

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GNAS	NP_001003263.1
Rattus norvegicus (Norway rat)	Gnas	NP_068617.6
Caenorhabditis elegans (roundworm)	gsa-1	NP_490817.3
Xenopus tropicalis (tropical clawed frog)	gnas	NP_998855.1
Pan troglodytes (chimpanzee)	GNAS	XP_001138016.3
Drosophila melanogaster (fruit fly)	G-salpha60A	NP_726388.1
Anopheles gambiae (African malaria mosquito)	GNAS_ANOGA	XP_320433.1
Bos taurus (cattle)	GNAS	NP_851364.1
Mus musculus (house mouse)	Gnas	NP_034439.2
Gallus gallus (chicken)	GNAS	XP_417485.3
Homo sapiens (human)	GNAS	NP_536350.2
Danio rerio (zebrafish)	gnas	XP_005172181.1
	GNAS	XP_001083687.2

Gene Ontology
Bibliography

Related articles in PubMed

Interference with Gs
Lachmann P, Hickmann L, Steglich A, Al-Mekhlafi M, Gerlach M, Jetschin N, Jahn S, Hamann B, Wnuk M, Madsen K, Djonov V, Chen M, Weinstein LS, Hohenstein B, Hugo CPM, Todorov VT
Journal of the American Society of Nephrology : JASN28(12)3479-3489(2017 Dec)

Internalized TSH receptors en route to the TGN induce local G
Godbole A, Lyga S, Lohse MJ, Calebiro D
Nature communications8(1)443(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gsalpha deficiency in JG cells of adult mice results in kidney injury, suggesting that JG cells are critically involved in the maintenance and protection of the renal microvascular endothelium.
Title: Interference with Gs

retrograde trafficking to the trans-Golgi network induces local Gs-protein activation and cAMP/PKA signaling at a critical position near the nucleus, which appears required for efficient CREB phosphorylation and gene transcription
Title: Internalized TSH receptors en route to the TGN induce local G

Disruption of Gnas in smooth muscle of mice reduced intestinal motility and led to death within 4 weeks. GNAS disruption in adults impaired contraction of intestinal smooth muscle and peristalsis with features of intestinal pseudo-obstruction characterized by chronic intestinal dilation and dysmotility.
Title: Heterotrimeric G Stimulatory Protein ? Subunit Is Required for?Intestinal Smooth Muscle Contraction in Mice.

results show that Gsalpha imprinting in the dorsomedial nucleus of the hypothalamus (DMH) underlies the parent-of-origin metabolic phenotype that results from Gsalpha mutations and that DMH MC4R/Gsalpha signaling is important for regulation of energy expenditure and brown adipose tissue activation, but not the metabolic response to cold.
Title: Gs? deficiency in the dorsomedial hypothalamus underlies obesity associated with Gs? mutations.

results suggest that the T1R3 homomeric sweet taste receptor negatively regulates adipogenesis through Galphas-mediated microtubule disassembly and consequent activation of the Rho/ROCK pathway.
Title: T1R3 homomeric sweet taste receptor regulates adipogenesis through G?s-mediated microtubules disassembly and Rho activation in 3T3-L1 cells.

The following Gnas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21778	NM_001077510.5 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu30030	NM_001364030.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu30030	XM_006498776.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu30031	XM_006498777.2 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu21780	NM_001310083.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu21780	NM_201616.3 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu21719	XM_006498779.4 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu21780	XM_030247568.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu30029	XM_006498774.2 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu30032	XM_006498778.3 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu80072	XM_017315658.2 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu87723	XM_030247569.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu21828	NM_201618.2 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu21786	NM_010309.4 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu21797	NM_001077507.2 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu30033	XM_006498780.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu21719	NM_022000.3 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu21719	NM_019690.3 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu21738	NM_201617.2 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu67835	NM_001310085.1 Latest version!	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu21778	NM_001077510.4	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu30032	XM_006498778.2	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu80072	XM_017315658.1	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu21780	NM_201616.2	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu30029	XM_006498774.1	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu21719	XM_006498779.3	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

Gnas ( NM_001077510.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001070978.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OMu21778
Clone ID Related Accession (Same CDS sequence)	NM_001077510.4 , NM_001077510.5
Accession Version	NM_001077510.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform GNASS
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK159563.1, BU054033.1, DV057819.1 and BC062654.1. On Jun 26, 2015 this sequence version replaced NM_001077510.3. Transcript Variant: This variant (8) is biallelically expressed. It lacks an internal exon and uses an alternate splice site, compared to variant 7. It encodes the isoform GNASS, also known as alpha-S1, which lacks an internal segment and has two amino acids different, compared to isoform GNASL. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATAGTGAGA AGGCCACTAA AGTGCAGGAC 
ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG CCGCCATGAG CAACCTGGTG 
CCCCCTGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG TGGACTACAT TCTGAGCGTG 
ATGAACGTGC CGAACTTTGA CTTCCCACCT GAATTCTATG AGCATGCCAA GGCTCTGTGG 
GAGGATGAGG GAGTGCGTGC CTGCTACGAG CGCTCCAATG AGTACCAGCT GATTGACTGT 
GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG CCGACTACGT GCCAAGTGAC 
CAGGACCTGC TTCGCTGCCG TGTCCTGACC TCTGGAATCT TTGAGACCAA GTTCCAGGTG 
GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC GCGATGAGCG CCGCAAGTGG 
ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG TGGCCAGCAG CAGCTACAAC 
ATGGTCATTC GGGAGGACAA CCAGACTAAC CGCCTGCAGG AGGCTCTGAA CCTCTTCAAG 
AGCATCTGGA ACAACAGATG GCTGCGCACC ATCTCTGTGA TTCTCTTCCT CAACAAGCAA 
GACCTGCTTG CTGAGAAAGT CCTCGCTGGC AAATCGAAGA TTGAGGACTA CTTTCCAGAG 
TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC CGGGAGAGGA CCCACGCGTG 
ACCCGGGCCA AGTACTTCAT TCGGGATGAG TTTCTGAGAA TCAGCACTGC TAGTGGAGAT 
GGGCGCCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG ACACTGAGAA CATCCGCCGT 
GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC TCCGCCAATA CGAGCTGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070978.1
CDS	208..1350
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001077510.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077510.4

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATAGTGAGA AGGCCACTAA AGTGCAGGAC 
ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG CCGCCATGAG CAACCTGGTG 
CCCCCTGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG TGGACTACAT TCTGAGCGTG 
ATGAACGTGC CGAACTTTGA CTTCCCACCT GAATTCTATG AGCATGCCAA GGCTCTGTGG 
GAGGATGAGG GAGTGCGTGC CTGCTACGAG CGCTCCAATG AGTACCAGCT GATTGACTGT 
GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG CCGACTACGT GCCAAGTGAC 
CAGGACCTGC TTCGCTGCCG TGTCCTGACC TCTGGAATCT TTGAGACCAA GTTCCAGGTG 
GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC GCGATGAGCG CCGCAAGTGG 
ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG TGGCCAGCAG CAGCTACAAC 
ATGGTCATTC GGGAGGACAA CCAGACTAAC CGCCTGCAGG AGGCTCTGAA CCTCTTCAAG 
AGCATCTGGA ACAACAGATG GCTGCGCACC ATCTCTGTGA TTCTCTTCCT CAACAAGCAA 
GACCTGCTTG CTGAGAAAGT CCTCGCTGGC AAATCGAAGA TTGAGGACTA CTTTCCAGAG 
TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC CGGGAGAGGA CCCACGCGTG 
ACCCGGGCCA AGTACTTCAT TCGGGATGAG TTTCTGAGAA TCAGCACTGC TAGTGGAGAT 
GGGCGCCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG ACACTGAGAA CATCCGCCGT 
GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC TCCGCCAATA CGAGCTGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001077510.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001070978.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OMu21778
Clone ID Related Accession (Same CDS sequence)	NM_001077510.4 , NM_001077510.5
Accession Version	NM_001077510.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform GNASS
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10, AK159563.1, BU054033.1, DV057819.1 and BC062654.1. On Jun 6, 2018 this sequence version replaced NM_001077510.4. Transcript Variant: This variant (8) is biallelically expressed. It lacks an internal exon and uses an alternate splice site, compared to variant 7. It encodes the isoform GNASS, also known as alpha-S1, which lacks an internal segment and has two amino acids different, compared to isoform GNASL. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061496.1, AK147051.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATAGTGAGA AGGCCACTAA AGTGCAGGAC 
ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG CCGCCATGAG CAACCTGGTG 
CCCCCTGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG TGGACTACAT TCTGAGCGTG 
ATGAACGTGC CGAACTTTGA CTTCCCACCT GAATTCTATG AGCATGCCAA GGCTCTGTGG 
GAGGATGAGG GAGTGCGTGC CTGCTACGAG CGCTCCAATG AGTACCAGCT GATTGACTGT 
GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG CCGACTACGT GCCAAGTGAC 
CAGGACCTGC TTCGCTGCCG TGTCCTGACC TCTGGAATCT TTGAGACCAA GTTCCAGGTG 
GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC GCGATGAGCG CCGCAAGTGG 
ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG TGGCCAGCAG CAGCTACAAC 
ATGGTCATTC GGGAGGACAA CCAGACTAAC CGCCTGCAGG AGGCTCTGAA CCTCTTCAAG 
AGCATCTGGA ACAACAGATG GCTGCGCACC ATCTCTGTGA TTCTCTTCCT CAACAAGCAA 
GACCTGCTTG CTGAGAAAGT CCTCGCTGGC AAATCGAAGA TTGAGGACTA CTTTCCAGAG 
TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC CGGGAGAGGA CCCACGCGTG 
ACCCGGGCCA AGTACTTCAT TCGGGATGAG TTTCTGAGAA TCAGCACTGC TAGTGGAGAT 
GGGCGCCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG ACACTGAGAA CATCCGCCGT 
GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC TCCGCCAATA CGAGCTGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070978.1
CDS	291..1433
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001077510.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077510.5

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATAGTGAGA AGGCCACTAA AGTGCAGGAC 
ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG CCGCCATGAG CAACCTGGTG 
CCCCCTGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG TGGACTACAT TCTGAGCGTG 
ATGAACGTGC CGAACTTTGA CTTCCCACCT GAATTCTATG AGCATGCCAA GGCTCTGTGG 
GAGGATGAGG GAGTGCGTGC CTGCTACGAG CGCTCCAATG AGTACCAGCT GATTGACTGT 
GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG CCGACTACGT GCCAAGTGAC 
CAGGACCTGC TTCGCTGCCG TGTCCTGACC TCTGGAATCT TTGAGACCAA GTTCCAGGTG 
GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC GCGATGAGCG CCGCAAGTGG 
ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG TGGCCAGCAG CAGCTACAAC 
ATGGTCATTC GGGAGGACAA CCAGACTAAC CGCCTGCAGG AGGCTCTGAA CCTCTTCAAG 
AGCATCTGGA ACAACAGATG GCTGCGCACC ATCTCTGTGA TTCTCTTCCT CAACAAGCAA 
GACCTGCTTG CTGAGAAAGT CCTCGCTGGC AAATCGAAGA TTGAGGACTA CTTTCCAGAG 
TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC CGGGAGAGGA CCCACGCGTG 
ACCCGGGCCA AGTACTTCAT TCGGGATGAG TTTCTGAGAA TCAGCACTGC TAGTGGAGAT 
GGGCGCCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG ACACTGAGAA CATCCGCCGT 
GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC TCCGCCAATA CGAGCTGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001364030.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350959.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 12, mRNA.

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CloneID	OMu30030
Clone ID Related Accession (Same CDS sequence)	NM_001364030.1 , XM_006498776.1
Accession Version	NM_001364030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	protein ALEX isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10. On Jun 6, 2018 this sequence version replaced XM_006498776.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M13964.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATGAGAAGG CCACTAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCTGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCGA ACTTTGACTT CCCACCTGAA TTCTATGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TGCGTGCCTG CTACGAGCGC TCCAATGAGT ACCAGCTGAT TGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGTGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAGCGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATTCGGG AGGACAACCA GACTAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGCACCATC TCTGTGATTC TCTTCCTCAA CAAGCAAGAC 
CTGCTTGCTG AGAAAGTCCT CGCTGGCAAA TCGAAGATTG AGGACTACTT TCCAGAGTTC 
GCTCGCTACA CCACTCCTGA GGATGCGACT CCCGAGCCGG GAGAGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATTCG GGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGG 
CGCCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTCC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350959.1
CDS	291..1430
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001364030.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364030.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATGAGAAGG CCACTAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCTGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCGA ACTTTGACTT CCCACCTGAA TTCTATGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TGCGTGCCTG CTACGAGCGC TCCAATGAGT ACCAGCTGAT TGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGTGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAGCGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATTCGGG AGGACAACCA GACTAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGCACCATC TCTGTGATTC TCTTCCTCAA CAAGCAAGAC 
CTGCTTGCTG AGAAAGTCCT CGCTGGCAAA TCGAAGATTG AGGACTACTT TCCAGAGTTC 
GCTCGCTACA CCACTCCTGA GGATGCGACT CCCGAGCCGG GAGAGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATTCG GGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGG 
CGCCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTCC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498774.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498837.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X2, mRNA.

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CloneID	OMu30029
Clone ID Related Accession (Same CDS sequence)	XM_006498774.1 , XM_006498774.2
Accession Version	XM_006498774.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTAG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498837.1
CDS	468..1655
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498774.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498774.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTAG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498776.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498839.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30030
Clone ID Related Accession (Same CDS sequence)	NM_001364030.1 , XM_006498776.1
Accession Version	XM_006498776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATGAGAAGG CCACTAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCTGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCGA ACTTTGACTT CCCACCTGAA TTCTATGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TGCGTGCCTG CTACGAGCGC TCCAATGAGT ACCAGCTGAT TGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGTGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAGCGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATTCGGG AGGACAACCA GACTAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGCACCATC TCTGTGATTC TCTTCCTCAA CAAGCAAGAC 
CTGCTTGCTG AGAAAGTCCT CGCTGGCAAA TCGAAGATTG AGGACTACTT TCCAGAGTTC 
GCTCGCTACA CCACTCCTGA GGATGCGACT CCCGAGCCGG GAGAGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATTCG GGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGG 
CGCCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTCC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498839.1
CDS	468..1607
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498776.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498776.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GATGAGAAGG CCACTAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCTGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCGA ACTTTGACTT CCCACCTGAA TTCTATGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TGCGTGCCTG CTACGAGCGC TCCAATGAGT ACCAGCTGAT TGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGTGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAGCGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATTCGGG AGGACAACCA GACTAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGCACCATC TCTGTGATTC TCTTCCTCAA CAAGCAAGAC 
CTGCTTGCTG AGAAAGTCCT CGCTGGCAAA TCGAAGATTG AGGACTACTT TCCAGAGTTC 
GCTCGCTACA CCACTCCTGA GGATGCGACT CCCGAGCCGG GAGAGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATTCG GGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGG 
CGCCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTCC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498777.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498840.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu30031
Clone ID Related Accession (Same CDS sequence)	XM_006498777.2
Accession Version	XM_006498777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACT CCGTCCAGAT TCTCCTTGTT TTCATGGATA AAGGTGCTGG AGAGTCTGGC 
AAAAGCACCA TTGTGAAGCA GATGAGGATC CTGCATGTTA ATGGGTTTAA CGGAGAGGGC 
GGCGAAGAGG ACCCGCAGGC TGCAAGGAGC AACAGCGATG GTAGTGAGAA GGCCACTAAA 
GTGCAGGACA TCAAAAACAA CCTGAAGGAG GCCATTGAAA CCATTGTGGC CGCCATGAGC 
AACCTGGTGC CCCCTGTGGA GCTGGCCAAC CCTGAGAACC AGTTCAGAGT GGACTACATT 
CTGAGCGTGA TGAACGTGCC GAACTTTGAC TTCCCACCTG AATTCTATGA GCATGCCAAG 
GCTCTGTGGG AGGATGAGGG AGTGCGTGCC TGCTACGAGC GCTCCAATGA GTACCAGCTG 
ATTGACTGTG CCCAGTACTT CCTGGACAAG ATTGATGTGA TCAAGCAGGC CGACTACGTG 
CCAAGTGACC AGGACCTGCT TCGCTGCCGT GTCCTGACCT CTGGAATCTT TGAGACCAAG 
TTCCAGGTGG ACAAAGTCAA CTTCCACATG TTCGATGTGG GCGGCCAGCG CGATGAGCGC 
CGCAAGTGGA TCCAGTGCTT CAATGATGTG ACTGCCATCA TCTTCGTGGT GGCCAGCAGC 
AGCTACAACA TGGTCATTCG GGAGGACAAC CAGACTAACC GCCTGCAGGA GGCTCTGAAC 
CTCTTCAAGA GCATCTGGAA CAACAGATGG CTGCGCACCA TCTCTGTGAT TCTCTTCCTC 
AACAAGCAAG ACCTGCTTGC TGAGAAAGTC CTCGCTGGCA AATCGAAGAT TGAGGACTAC 
TTTCCAGAGT TCGCTCGCTA CACCACTCCT GAGGATGCGA CTCCCGAGCC GGGAGAGGAC 
CCACGCGTGA CCCGGGCCAA GTACTTCATT CGGGATGAGT TTCTGAGAAT CAGCACTGCT 
AGTGGAGATG GGCGCCACTA CTGCTACCCT CACTTTACCT GCGCCGTGGA CACTGAGAAC 
ATCCGCCGTG TCTTCAACGA CTGCCGTGAC ATCATCCAGC GCATGCATCT CCGCCAATAC 
GAGCTGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498840.1
CDS	37..1128
Translation	MGDSVQILLVFMDKGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498777.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498777.2

ORF Insert Sequence:

ATGGGTGACT CCGTCCAGAT TCTCCTTGTT TTCATGGATA AAGGTGCTGG AGAGTCTGGC 
AAAAGCACCA TTGTGAAGCA GATGAGGATC CTGCATGTTA ATGGGTTTAA CGGAGAGGGC 
GGCGAAGAGG ACCCGCAGGC TGCAAGGAGC AACAGCGATG GTAGTGAGAA GGCCACTAAA 
GTGCAGGACA TCAAAAACAA CCTGAAGGAG GCCATTGAAA CCATTGTGGC CGCCATGAGC 
AACCTGGTGC CCCCTGTGGA GCTGGCCAAC CCTGAGAACC AGTTCAGAGT GGACTACATT 
CTGAGCGTGA TGAACGTGCC GAACTTTGAC TTCCCACCTG AATTCTATGA GCATGCCAAG 
GCTCTGTGGG AGGATGAGGG AGTGCGTGCC TGCTACGAGC GCTCCAATGA GTACCAGCTG 
ATTGACTGTG CCCAGTACTT CCTGGACAAG ATTGATGTGA TCAAGCAGGC CGACTACGTG 
CCAAGTGACC AGGACCTGCT TCGCTGCCGT GTCCTGACCT CTGGAATCTT TGAGACCAAG 
TTCCAGGTGG ACAAAGTCAA CTTCCACATG TTCGATGTGG GCGGCCAGCG CGATGAGCGC 
CGCAAGTGGA TCCAGTGCTT CAATGATGTG ACTGCCATCA TCTTCGTGGT GGCCAGCAGC 
AGCTACAACA TGGTCATTCG GGAGGACAAC CAGACTAACC GCCTGCAGGA GGCTCTGAAC 
CTCTTCAAGA GCATCTGGAA CAACAGATGG CTGCGCACCA TCTCTGTGAT TCTCTTCCTC 
AACAAGCAAG ACCTGCTTGC TGAGAAAGTC CTCGCTGGCA AATCGAAGAT TGAGGACTAC 
TTTCCAGAGT TCGCTCGCTA CACCACTCCT GAGGATGCGA CTCCCGAGCC GGGAGAGGAC 
CCACGCGTGA CCCGGGCCAA GTACTTCATT CGGGATGAGT TTCTGAGAAT CAGCACTGCT 
AGTGGAGATG GGCGCCACTA CTGCTACCCT CACTTTACCT GCGCCGTGGA CACTGAGAAC 
ATCCGCCGTG TCTTCAACGA CTGCCGTGAC ATCATCCAGC GCATGCATCT CCGCCAATAC 
GAGCTGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498778.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498841.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X5, mRNA.

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CloneID	OMu30032
Clone ID Related Accession (Same CDS sequence)	XM_006498778.3 , XM_006498778.2
Accession Version	XM_006498778.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACT CCGTCCAGAT TCTCCTTGTT TTCATGGATA AAGGTGCTGG AGAGTCTGGC 
AAAAGCACCA TTGTGAAGCA GATGAGGATC CTGCATGTTA ATGGGTTTAA CGGAGATGAG 
AAGGCCACTA AAGTGCAGGA CATCAAAAAC AACCTGAAGG AGGCCATTGA AACCATTGTG 
GCCGCCATGA GCAACCTGGT GCCCCCTGTG GAGCTGGCCA ACCCTGAGAA CCAGTTCAGA 
GTGGACTACA TTCTGAGCGT GATGAACGTG CCGAACTTTG ACTTCCCACC TGAATTCTAT 
GAGCATGCCA AGGCTCTGTG GGAGGATGAG GGAGTGCGTG CCTGCTACGA GCGCTCCAAT 
GAGTACCAGC TGATTGACTG TGCCCAGTAC TTCCTGGACA AGATTGATGT GATCAAGCAG 
GCCGACTACG TGCCAAGTGA CCAGGACCTG CTTCGCTGCC GTGTCCTGAC CTCTGGAATC 
TTTGAGACCA AGTTCCAGGT GGACAAAGTC AACTTCCACA TGTTCGATGT GGGCGGCCAG 
CGCGATGAGC GCCGCAAGTG GATCCAGTGC TTCAATGATG TGACTGCCAT CATCTTCGTG 
GTGGCCAGCA GCAGCTACAA CATGGTCATT CGGGAGGACA ACCAGACTAA CCGCCTGCAG 
GAGGCTCTGA ACCTCTTCAA GAGCATCTGG AACAACAGAT GGCTGCGCAC CATCTCTGTG 
ATTCTCTTCC TCAACAAGCA AGACCTGCTT GCTGAGAAAG TCCTCGCTGG CAAATCGAAG 
ATTGAGGACT ACTTTCCAGA GTTCGCTCGC TACACCACTC CTGAGGATGC GACTCCCGAG 
CCGGGAGAGG ACCCACGCGT GACCCGGGCC AAGTACTTCA TTCGGGATGA GTTTCTGAGA 
ATCAGCACTG CTAGTGGAGA TGGGCGCCAC TACTGCTACC CTCACTTTAC CTGCGCCGTG 
GACACTGAGA ACATCCGCCG TGTCTTCAAC GACTGCCGTG ACATCATCCA GCGCATGCAT 
CTCCGCCAAT ACGAGCTGCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498841.1
CDS	37..1080
Translation	MGDSVQILLVFMDKGAGESGKSTIVKQMRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498778.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498778.2

ORF Insert Sequence:

ATGGGTGACT CCGTCCAGAT TCTCCTTGTT TTCATGGATA AAGGTGCTGG AGAGTCTGGC 
AAAAGCACCA TTGTGAAGCA GATGAGGATC CTGCATGTTA ATGGGTTTAA CGGAGATGAG 
AAGGCCACTA AAGTGCAGGA CATCAAAAAC AACCTGAAGG AGGCCATTGA AACCATTGTG 
GCCGCCATGA GCAACCTGGT GCCCCCTGTG GAGCTGGCCA ACCCTGAGAA CCAGTTCAGA 
GTGGACTACA TTCTGAGCGT GATGAACGTG CCGAACTTTG ACTTCCCACC TGAATTCTAT 
GAGCATGCCA AGGCTCTGTG GGAGGATGAG GGAGTGCGTG CCTGCTACGA GCGCTCCAAT 
GAGTACCAGC TGATTGACTG TGCCCAGTAC TTCCTGGACA AGATTGATGT GATCAAGCAG 
GCCGACTACG TGCCAAGTGA CCAGGACCTG CTTCGCTGCC GTGTCCTGAC CTCTGGAATC 
TTTGAGACCA AGTTCCAGGT GGACAAAGTC AACTTCCACA TGTTCGATGT GGGCGGCCAG 
CGCGATGAGC GCCGCAAGTG GATCCAGTGC TTCAATGATG TGACTGCCAT CATCTTCGTG 
GTGGCCAGCA GCAGCTACAA CATGGTCATT CGGGAGGACA ACCAGACTAA CCGCCTGCAG 
GAGGCTCTGA ACCTCTTCAA GAGCATCTGG AACAACAGAT GGCTGCGCAC CATCTCTGTG 
ATTCTCTTCC TCAACAAGCA AGACCTGCTT GCTGAGAAAG TCCTCGCTGG CAAATCGAAG 
ATTGAGGACT ACTTTCCAGA GTTCGCTCGC TACACCACTC CTGAGGATGC GACTCCCGAG 
CCGGGAGAGG ACCCACGCGT GACCCGGGCC AAGTACTTCA TTCGGGATGA GTTTCTGAGA 
ATCAGCACTG CTAGTGGAGA TGGGCGCCAC TACTGCTACC CTCACTTTAC CTGCGCCGTG 
GACACTGAGA ACATCCGCCG TGTCTTCAAC GACTGCCGTG ACATCATCCA GCGCATGCAT 
CTCCGCCAAT ACGAGCTGCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_017315658.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171147.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X6, mRNA.

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CloneID	OMu80072
Clone ID Related Accession (Same CDS sequence)	XM_017315658.2 , XM_017315658.1
Accession Version	XM_017315658.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATCC TGCATGTTAA TGGGTTTAAC GGAGAGGGCG GCGAAGAGGA CCCGCAGGCT 
GCAAGGAGCA ACAGCGATGG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171147.1
CDS	238..1245
Translation	MRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_017315658.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315658.1

ORF Insert Sequence:

ATGAGGATCC TGCATGTTAA TGGGTTTAAC GGAGAGGGCG GCGAAGAGGA CCCGCAGGCT 
GCAAGGAGCA ACAGCGATGG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001310083.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297012.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 10, mRNA.

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CloneID	OMu21780
Clone ID Related Accession (Same CDS sequence)	NM_001310083.1 , NM_201616.3 , NM_201616.2 , XM_030247568.1
Accession Version	NM_001310083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform GNASL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062654.1, BM944582.1 and BU516278.1. On Jun 26, 2015 this sequence version replaced XM_006498775.1. Transcript Variant: This variant (10) differs in the 5' UTR, compared to variant 7. Variants 7 and 10 encode the same isoform guanine nucleotide binding protein alpha-s long (GNASL, also known as alpha-S2), a form of the G-protein alpha subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297012.1
CDS	2363..3547
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001310083.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310083.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_201616.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963910.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 7, mRNA.

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CloneID	OMu21780
Clone ID Related Accession (Same CDS sequence)	NM_001310083.1 , NM_201616.3 , NM_201616.2 , XM_030247568.1
Accession Version	NM_201616.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform GNASL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10, CK625094.1, AK159563.1, AK168996.1 and BU516278.1. On Jun 6, 2018 this sequence version replaced NM_201616.2. Transcript Variant: This variant (7) is biallelically expressed and encodes guanine nucleotide binding protein alpha-s long (GNASL) isoform, also known as alpha-S2, a form of the G-protein alpha subunit. Variants 7 and 10 encode the same isoform (GNASL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092055.1, AK078037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963910.1
CDS	291..1475
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_201616.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201616.3

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_201616.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963910.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 7, mRNA.

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CloneID	OMu21780
Clone ID Related Accession (Same CDS sequence)	NM_001310083.1 , NM_201616.3 , NM_201616.2 , XM_030247568.1
Accession Version	NM_201616.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform GNASL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK625094.1, AK159563.1, AK168996.1 and BU516278.1. On Jun 20, 2015 this sequence version replaced NM_201616.1. Transcript Variant: This variant (7) is biallelically expressed and encodes guanine nucleotide binding protein alpha-s long (GNASL) isoform, also known as alpha-S2, a form of the G-protein alpha subunit. Variants 7 and 10 encode the same isoform (GNASL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963910.1
CDS	227..1411
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_201616.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201616.2

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498779.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498842.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X10, mRNA.

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CloneID	OMu21719
Clone ID Related Accession (Same CDS sequence)	NM_022000.3 , XM_006498779.3 , NM_019690.3 , XM_006498779.4
Accession Version	XM_006498779.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SCG6 (secretogranin VI) isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498842.1
CDS	260..1021
Translation	MDRRSRAQQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAAQRRSFLNAHHRSAAAAAAAQVLPESSESESDHEHEEVEPELARPECLEYDQDDYETETDSETEPESDIESETEIETEPETEPETEPETEPEDERGPRGATFNQSLTQRLHALKLQSADASPRRAQPTTQEPESASEGEEPQRGPLDQDPRDPEEEPEERKEENRQPRRCKTRRPARRRDQSPESPPRKGPIPIRRH

Target ORF information:

RefSeq Version	XM_006498779.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498779.4

ORF Insert Sequence:

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_030247568.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103428.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X3, mRNA.

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CloneID	OMu21780
Clone ID Related Accession (Same CDS sequence)	NM_001310083.1 , NM_201616.3 , NM_201616.2 , XM_030247568.1
Accession Version	XM_030247568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103428.1
CDS	2203..3387
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_030247568.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247568.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACT AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCTGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCGAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTGCGT 
GCCTGCTACG AGCGCTCCAA TGAGTACCAG CTGATTGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGTGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAG CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT TCGGGAGGAC 
AACCAGACTA ACCGCCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGCA CCATCTCTGT GATTCTCTTC CTCAACAAGC AAGACCTGCT TGCTGAGAAA 
GTCCTCGCTG GCAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCGGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATTCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGGCGCCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTCCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498774.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498837.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X2, mRNA.

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CloneID	OMu30029
Clone ID Related Accession (Same CDS sequence)	XM_006498774.1 , XM_006498774.2
Accession Version	XM_006498774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTAG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498837.1
CDS	522..1709
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498774.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498774.2

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTGC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTAG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498778.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498841.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X5, mRNA.

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CloneID	OMu30032
Clone ID Related Accession (Same CDS sequence)	XM_006498778.3 , XM_006498778.2
Accession Version	XM_006498778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACT CCGTCCAGAT TCTCCTTGTT TTCATGGATA AAGGTGCTGG AGAGTCTGGC 
AAAAGCACCA TTGTGAAGCA GATGAGGATC CTGCATGTTA ATGGGTTTAA CGGAGATGAG 
AAGGCCACTA AAGTGCAGGA CATCAAAAAC AACCTGAAGG AGGCCATTGA AACCATTGTG 
GCCGCCATGA GCAACCTGGT GCCCCCTGTG GAGCTGGCCA ACCCTGAGAA CCAGTTCAGA 
GTGGACTACA TTCTGAGCGT GATGAACGTG CCGAACTTTG ACTTCCCACC TGAATTCTAT 
GAGCATGCCA AGGCTCTGTG GGAGGATGAG GGAGTGCGTG CCTGCTACGA GCGCTCCAAT 
GAGTACCAGC TGATTGACTG TGCCCAGTAC TTCCTGGACA AGATTGATGT GATCAAGCAG 
GCCGACTACG TGCCAAGTGA CCAGGACCTG CTTCGCTGCC GTGTCCTGAC CTCTGGAATC 
TTTGAGACCA AGTTCCAGGT GGACAAAGTC AACTTCCACA TGTTCGATGT GGGCGGCCAG 
CGCGATGAGC GCCGCAAGTG GATCCAGTGC TTCAATGATG TGACTGCCAT CATCTTCGTG 
GTGGCCAGCA GCAGCTACAA CATGGTCATT CGGGAGGACA ACCAGACTAA CCGCCTGCAG 
GAGGCTCTGA ACCTCTTCAA GAGCATCTGG AACAACAGAT GGCTGCGCAC CATCTCTGTG 
ATTCTCTTCC TCAACAAGCA AGACCTGCTT GCTGAGAAAG TCCTCGCTGG CAAATCGAAG 
ATTGAGGACT ACTTTCCAGA GTTCGCTCGC TACACCACTC CTGAGGATGC GACTCCCGAG 
CCGGGAGAGG ACCCACGCGT GACCCGGGCC AAGTACTTCA TTCGGGATGA GTTTCTGAGA 
ATCAGCACTG CTAGTGGAGA TGGGCGCCAC TACTGCTACC CTCACTTTAC CTGCGCCGTG 
GACACTGAGA ACATCCGCCG TGTCTTCAAC GACTGCCGTG ACATCATCCA GCGCATGCAT 
CTCCGCCAAT ACGAGCTGCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498841.1
CDS	151..1194
Translation	MGDSVQILLVFMDKGAGESGKSTIVKQMRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498778.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498778.3

ORF Insert Sequence:

ATGGGTGACT CCGTCCAGAT TCTCCTTGTT TTCATGGATA AAGGTGCTGG AGAGTCTGGC 
AAAAGCACCA TTGTGAAGCA GATGAGGATC CTGCATGTTA ATGGGTTTAA CGGAGATGAG 
AAGGCCACTA AAGTGCAGGA CATCAAAAAC AACCTGAAGG AGGCCATTGA AACCATTGTG 
GCCGCCATGA GCAACCTGGT GCCCCCTGTG GAGCTGGCCA ACCCTGAGAA CCAGTTCAGA 
GTGGACTACA TTCTGAGCGT GATGAACGTG CCGAACTTTG ACTTCCCACC TGAATTCTAT 
GAGCATGCCA AGGCTCTGTG GGAGGATGAG GGAGTGCGTG CCTGCTACGA GCGCTCCAAT 
GAGTACCAGC TGATTGACTG TGCCCAGTAC TTCCTGGACA AGATTGATGT GATCAAGCAG 
GCCGACTACG TGCCAAGTGA CCAGGACCTG CTTCGCTGCC GTGTCCTGAC CTCTGGAATC 
TTTGAGACCA AGTTCCAGGT GGACAAAGTC AACTTCCACA TGTTCGATGT GGGCGGCCAG 
CGCGATGAGC GCCGCAAGTG GATCCAGTGC TTCAATGATG TGACTGCCAT CATCTTCGTG 
GTGGCCAGCA GCAGCTACAA CATGGTCATT CGGGAGGACA ACCAGACTAA CCGCCTGCAG 
GAGGCTCTGA ACCTCTTCAA GAGCATCTGG AACAACAGAT GGCTGCGCAC CATCTCTGTG 
ATTCTCTTCC TCAACAAGCA AGACCTGCTT GCTGAGAAAG TCCTCGCTGG CAAATCGAAG 
ATTGAGGACT ACTTTCCAGA GTTCGCTCGC TACACCACTC CTGAGGATGC GACTCCCGAG 
CCGGGAGAGG ACCCACGCGT GACCCGGGCC AAGTACTTCA TTCGGGATGA GTTTCTGAGA 
ATCAGCACTG CTAGTGGAGA TGGGCGCCAC TACTGCTACC CTCACTTTAC CTGCGCCGTG 
GACACTGAGA ACATCCGCCG TGTCTTCAAC GACTGCCGTG ACATCATCCA GCGCATGCAT 
CTCCGCCAAT ACGAGCTGCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_017315658.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171147.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X6, mRNA.

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CloneID	OMu80072
Clone ID Related Accession (Same CDS sequence)	XM_017315658.2 , XM_017315658.1
Accession Version	XM_017315658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATCC TGCATGTTAA TGGGTTTAAC GGAGAGGGCG GCGAAGAGGA CCCGCAGGCT 
GCAAGGAGCA ACAGCGATGG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171147.1
CDS	305..1312
Translation	MRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_017315658.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315658.2

ORF Insert Sequence:

ATGAGGATCC TGCATGTTAA TGGGTTTAAC GGAGAGGGCG GCGAAGAGGA CCCGCAGGCT 
GCAAGGAGCA ACAGCGATGG TGAGAAGGCC ACTAAAGTGC AGGACATCAA AAACAACCTG 
AAGGAGGCCA TTGAAACCAT TGTGGCCGCC ATGAGCAACC TGGTGCCCCC TGTGGAGCTG 
GCCAACCCTG AGAACCAGTT CAGAGTGGAC TACATTCTGA GCGTGATGAA CGTGCCGAAC 
TTTGACTTCC CACCTGAATT CTATGAGCAT GCCAAGGCTC TGTGGGAGGA TGAGGGAGTG 
CGTGCCTGCT ACGAGCGCTC CAATGAGTAC CAGCTGATTG ACTGTGCCCA GTACTTCCTG 
GACAAGATTG ATGTGATCAA GCAGGCCGAC TACGTGCCAA GTGACCAGGA CCTGCTTCGC 
TGCCGTGTCC TGACCTCTGG AATCTTTGAG ACCAAGTTCC AGGTGGACAA AGTCAACTTC 
CACATGTTCG ATGTGGGCGG CCAGCGCGAT GAGCGCCGCA AGTGGATCCA GTGCTTCAAT 
GATGTGACTG CCATCATCTT CGTGGTGGCC AGCAGCAGCT ACAACATGGT CATTCGGGAG 
GACAACCAGA CTAACCGCCT GCAGGAGGCT CTGAACCTCT TCAAGAGCAT CTGGAACAAC 
AGATGGCTGC GCACCATCTC TGTGATTCTC TTCCTCAACA AGCAAGACCT GCTTGCTGAG 
AAAGTCCTCG CTGGCAAATC GAAGATTGAG GACTACTTTC CAGAGTTCGC TCGCTACACC 
ACTCCTGAGG ATGCGACTCC CGAGCCGGGA GAGGACCCAC GCGTGACCCG GGCCAAGTAC 
TTCATTCGGG ATGAGTTTCT GAGAATCAGC ACTGCTAGTG GAGATGGGCG CCACTACTGC 
TACCCTCACT TTACCTGCGC CGTGGACACT GAGAACATCC GCCGTGTCTT CAACGACTGC 
CGTGACATCA TCCAGCGCAT GCATCTCCGC CAATACGAGC TGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_030247569.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103429.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X7, mRNA.

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CloneID	OMu87723
Clone ID Related Accession (Same CDS sequence)	XM_030247569.1
Accession Version	XM_030247569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATCC TGCATGTTAA TGGGTTTAAC GGAGATGAGA AGGCCACTAA AGTGCAGGAC 
ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG CCGCCATGAG CAACCTGGTG 
CCCCCTGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG TGGACTACAT TCTGAGCGTG 
ATGAACGTGC CGAACTTTGA CTTCCCACCT GAATTCTATG AGCATGCCAA GGCTCTGTGG 
GAGGATGAGG GAGTGCGTGC CTGCTACGAG CGCTCCAATG AGTACCAGCT GATTGACTGT 
GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG CCGACTACGT GCCAAGTGAC 
CAGGACCTGC TTCGCTGCCG TGTCCTGACC TCTGGAATCT TTGAGACCAA GTTCCAGGTG 
GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC GCGATGAGCG CCGCAAGTGG 
ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG TGGCCAGCAG CAGCTACAAC 
ATGGTCATTC GGGAGGACAA CCAGACTAAC CGCCTGCAGG AGGCTCTGAA CCTCTTCAAG 
AGCATCTGGA ACAACAGATG GCTGCGCACC ATCTCTGTGA TTCTCTTCCT CAACAAGCAA 
GACCTGCTTG CTGAGAAAGT CCTCGCTGGC AAATCGAAGA TTGAGGACTA CTTTCCAGAG 
TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC CGGGAGAGGA CCCACGCGTG 
ACCCGGGCCA AGTACTTCAT TCGGGATGAG TTTCTGAGAA TCAGCACTGC TAGTGGAGAT 
GGGCGCCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG ACACTGAGAA CATCCGCCGT 
GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC TCCGCCAATA CGAGCTGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103429.1
CDS	224..1186
Translation	MRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_030247569.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247569.1

ORF Insert Sequence:

ATGAGGATCC TGCATGTTAA TGGGTTTAAC GGAGATGAGA AGGCCACTAA AGTGCAGGAC 
ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG CCGCCATGAG CAACCTGGTG 
CCCCCTGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG TGGACTACAT TCTGAGCGTG 
ATGAACGTGC CGAACTTTGA CTTCCCACCT GAATTCTATG AGCATGCCAA GGCTCTGTGG 
GAGGATGAGG GAGTGCGTGC CTGCTACGAG CGCTCCAATG AGTACCAGCT GATTGACTGT 
GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG CCGACTACGT GCCAAGTGAC 
CAGGACCTGC TTCGCTGCCG TGTCCTGACC TCTGGAATCT TTGAGACCAA GTTCCAGGTG 
GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC GCGATGAGCG CCGCAAGTGG 
ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG TGGCCAGCAG CAGCTACAAC 
ATGGTCATTC GGGAGGACAA CCAGACTAAC CGCCTGCAGG AGGCTCTGAA CCTCTTCAAG 
AGCATCTGGA ACAACAGATG GCTGCGCACC ATCTCTGTGA TTCTCTTCCT CAACAAGCAA 
GACCTGCTTG CTGAGAAAGT CCTCGCTGGC AAATCGAAGA TTGAGGACTA CTTTCCAGAG 
TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC CGGGAGAGGA CCCACGCGTG 
ACCCGGGCCA AGTACTTCAT TCGGGATGAG TTTCTGAGAA TCAGCACTGC TAGTGGAGAT 
GGGCGCCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG ACACTGAGAA CATCCGCCGT 
GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC TCCGCCAATA CGAGCTGCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_201618.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963912.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, mRNA.

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CloneID	OMu21828
Clone ID Related Accession (Same CDS sequence)	NM_201618.2
Accession Version	NM_201618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	protein ALEX isoform Alex
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10, AK132636.1 and BU516278.1. On Jun 25, 2015 this sequence version replaced NM_201618.1. Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, compared to variant 7. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents isoform Alex (also known as alexX), which has no similarity to other isoforms encoded by this gene. It interacts with isoform XLas, and their interaction is essential for G-protein signaling in neuroendocrine cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCGT CCCCGACGAG ACTGGCAGTG AGATCAGTGG ACCCCCAGAA GACTCCAAAT 
CTGACATCCA AAGCCCCTGC CAGGCCTTCG AGGAAGTCCG AGTGGGTGGA GACTACAGCC 
CACCTCCGGA GGAAGCCATG CCATTCGAGA CACAACAGCC CAGCCTGGGA GATTTCTGGC 
CCACCCTGGA GCAGCCAGGA CCATCTGGGA CCCCATCAGG CCTCCAAGCC TTCAACCCAG 
CGATTTTGGA GCCCGGGACC CCCACTGGCG CGAGCCCAGG CCTGGGAGCC TATACCCCCC 
CACCAGAAGA AGCTATGCCA TTTGAGTTCA ACGAGCCTGC CCAGGGAGAC CATAGCCAGC 
CTCCCTTGCA AGTCCCAGAC CTTGCGCCAG GAGGTCCGGA AGCATTGGTC CCCAGAGCTC 
TTCCCGCGGA GCCCGGGAAC ATCAGATTTG AAAACGCTGG CTTCCGAGAA GACTACAGCC 
CTCCCCCTGA AGAATCTGTG CCATTTCAGG TCGGTGGAGA AGAATTCGGG GGCGATAGCC 
CACCCCCAGG ACTCCCGCGA GTCATCCCAC AAATCGGCAT TGGCGGCGAG TTCCCGACAG 
TCGCGGTCCC GAGTGCGCTC TGCCTCGCTC CCGCCGAGAA CGCGCCTCCC CTCTGGGTCC 
GAGGCGCCAT TGACAGACCA TTCCGCGAGG CTGTCAGATC TCCTCCTAAC TTCGCATGCG 
ACAGCCCCCC GATGGAGATC ACCAGACCCC TGCTTGAGAT TGGCAGAGCC TCCATTGGGG 
TCGACGACGA CACCGCTGTC AATATGGACA GCCCCCCAAT CGCAAGTGAT GGCCCGCCCA 
TCGAAGTCTC GGGAGCCCCA GATAAGAGCG AGTGCGCAGA GAGACCCCCA GTTGAGCGAG 
AAGCAGCCGA GATGGAAGGA AGCCCTACCA CCGCCACTGC GGTGGAAGGA AAAGTCCCCT 
CTCCGGAGAG AGGGGACGGA TCTTCCACCC AGCCTGAAGC AATGGATGCC AAGCCAGCCC 
CTGCTGCCCA AGCCGTCTCT ACCGGATCTG ATGCTGGAGC TCCTACGGAT TCCGCGATGC 
TCACAGATAG CCAGAGCGAT GCCGGAGAAG ACGGGACAGC CCCAGGAACG CCTTCAGATC 
TCCAGTCGGA TCCTGAAGAA CTCGAAGAAG CCCCAGCTGT CCGCGCCGAT CCTGACGGAG 
GGGCAGCCCC AGTCGCCCCA GCCACTCCTG CCGAGTCCGA GTCTGAAGGC AGCAGAGATC 
CAGCCGCCGA GCCAGCCTCC GAGGCAGTCC CTGCCACCAC GGCCGAGTCT GCCTCCGGGG 
CAGCCCCTGT CACCCAGGTG GAGCCCGCAG CCGCGGCAGT CTCTGCCACC CTGGCGGAGC 
CTGCCGCCCG GGCAGCCCCT ATCACCCCCA AGGAGCCCAC TACCCGGGCA GTCCCCTCTG 
CTAGAGCCCA TCCGGCCGCT GGAGCAGTCC CTGGCGCCCC AGCAATGTCA GCCTCTGCTA 
GGGCAGCTGC CGCTAGGGCA GCCTATGCAG GTCCACTGGT CTGGGGAGCC AGGTCACTCT 
CAGCTACTCC CGCCGCTCGG GCATCCCTTC CTGCCCGCGC AGCAGCTGCC GCCCGGGCAG 
CCTCTGCTGC CCGCGCAGTC GCTGCTGGCC GGTCAGCCTC TGCCGCCCCC AGCAGGGCCC 
ATCTTAGACC CCCCAGCCCC GAGATCCAGG TTGCTGACCC GCCTACTCCG CGGCCTCCTC 
CGCGGCCGAC TGCCTGGCCT GACAAGTACG AGCGGGGCCG AAGCTGCTGC AGGTACGAGG 
CATCGTCTGG CATCTGCGAG ATCGAGTCCT CCAGTGATGA GTCGGAAGAA GGGGCCACCG 
GCTGCTTCCA GTGGCTTCTG CGGCGAAACC GCCGCCCTGG CCTGCCCCGG AGCCACACGG 
TCGGGAGCAA CCCAGTCCGC AACTTCTTCA CCCGAGCCTT CGGAAGCTGC TTCGGTCTAT 
CCGAGTGTAC CCGATCACGA TCCCTCAGCC CCGGGAAGGC CAAGGATCCT ATGGAGGAGA 
GGCGCAAACA GATGCGCAAA GAAGCCATTG AGATGCGAGA GCAGAAGCGC GCAGATAAGA 
AACGCAGCAA GCTCATCGAC AAGCAACTGG AGGAGGAGAA GATGGACTAC ATGTGTACAC 
ACCGCCTGCT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963912.1
CDS	182..2359
Translation	MSPSPTRLAVRSVDPQKTPNLTSKAPARPSRKSEWVETTAHLRRKPCHSRHNSPAWEISGPPWSSQDHLGPHQASKPSTQRFWSPGPPLARAQAWEPIPPHQKKLCHLSSTSLPRETIASLPCKSQTLRQEVRKHWSPELFPRSPGTSDLKTLASEKTTALPLKNLCHFRSVEKNSGAIAHPQDSRESSHKSALAASSRQSRSRVRSASLPPRTRLPSGSEAPLTDHSARLSDLLLTSHATAPRWRSPDPCLRLAEPPLGSTTTPLSIWTAPQSQVMARPSKSREPQIRASAQRDPQLSEKQPRWKEALPPPLRWKEKSPLRREGTDLPPSLKQWMPSQPLLPKPSLPDLMLELLRIPRCSQIARAMPEKTGQPQERLQISSRILKNSKKPQLSAPILTEGQPQSPQPLLPSPSLKAAEIQPPSQPPRQSLPPRPSLPPGQPLSPRWSPQPRQSLPPWRSLPPGQPLSPPRSPLPGQSPLLEPIRPLEQSLAPQQCQPLLGQLPLGQPMQVHWSGEPGHSQLLPPLGHPFLPAQQLPPGQPLLPAQSLLAGQPLPPPAGPILDPPAPRSRLLTRLLRGLLRGRLPGLTSTSGAEAAAGTRHRLASARSSPPVMSRKKGPPAASSGFCGETAALACPGATRSGATQSATSSPEPSEAASVYPSVPDHDPSAPGRPRILWRRGANRCAKKPLRCESRSAQIRNAASSSTSNWRRRRWTTCVHTACCF

Target ORF information:

RefSeq Version	NM_201618.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201618.2

ORF Insert Sequence:

ATGAGCCCGT CCCCGACGAG ACTGGCAGTG AGATCAGTGG ACCCCCAGAA GACTCCAAAT 
CTGACATCCA AAGCCCCTGC CAGGCCTTCG AGGAAGTCCG AGTGGGTGGA GACTACAGCC 
CACCTCCGGA GGAAGCCATG CCATTCGAGA CACAACAGCC CAGCCTGGGA GATTTCTGGC 
CCACCCTGGA GCAGCCAGGA CCATCTGGGA CCCCATCAGG CCTCCAAGCC TTCAACCCAG 
CGATTTTGGA GCCCGGGACC CCCACTGGCG CGAGCCCAGG CCTGGGAGCC TATACCCCCC 
CACCAGAAGA AGCTATGCCA TTTGAGTTCA ACGAGCCTGC CCAGGGAGAC CATAGCCAGC 
CTCCCTTGCA AGTCCCAGAC CTTGCGCCAG GAGGTCCGGA AGCATTGGTC CCCAGAGCTC 
TTCCCGCGGA GCCCGGGAAC ATCAGATTTG AAAACGCTGG CTTCCGAGAA GACTACAGCC 
CTCCCCCTGA AGAATCTGTG CCATTTCAGG TCGGTGGAGA AGAATTCGGG GGCGATAGCC 
CACCCCCAGG ACTCCCGCGA GTCATCCCAC AAATCGGCAT TGGCGGCGAG TTCCCGACAG 
TCGCGGTCCC GAGTGCGCTC TGCCTCGCTC CCGCCGAGAA CGCGCCTCCC CTCTGGGTCC 
GAGGCGCCAT TGACAGACCA TTCCGCGAGG CTGTCAGATC TCCTCCTAAC TTCGCATGCG 
ACAGCCCCCC GATGGAGATC ACCAGACCCC TGCTTGAGAT TGGCAGAGCC TCCATTGGGG 
TCGACGACGA CACCGCTGTC AATATGGACA GCCCCCCAAT CGCAAGTGAT GGCCCGCCCA 
TCGAAGTCTC GGGAGCCCCA GATAAGAGCG AGTGCGCAGA GAGACCCCCA GTTGAGCGAG 
AAGCAGCCGA GATGGAAGGA AGCCCTACCA CCGCCACTGC GGTGGAAGGA AAAGTCCCCT 
CTCCGGAGAG AGGGGACGGA TCTTCCACCC AGCCTGAAGC AATGGATGCC AAGCCAGCCC 
CTGCTGCCCA AGCCGTCTCT ACCGGATCTG ATGCTGGAGC TCCTACGGAT TCCGCGATGC 
TCACAGATAG CCAGAGCGAT GCCGGAGAAG ACGGGACAGC CCCAGGAACG CCTTCAGATC 
TCCAGTCGGA TCCTGAAGAA CTCGAAGAAG CCCCAGCTGT CCGCGCCGAT CCTGACGGAG 
GGGCAGCCCC AGTCGCCCCA GCCACTCCTG CCGAGTCCGA GTCTGAAGGC AGCAGAGATC 
CAGCCGCCGA GCCAGCCTCC GAGGCAGTCC CTGCCACCAC GGCCGAGTCT GCCTCCGGGG 
CAGCCCCTGT CACCCAGGTG GAGCCCGCAG CCGCGGCAGT CTCTGCCACC CTGGCGGAGC 
CTGCCGCCCG GGCAGCCCCT ATCACCCCCA AGGAGCCCAC TACCCGGGCA GTCCCCTCTG 
CTAGAGCCCA TCCGGCCGCT GGAGCAGTCC CTGGCGCCCC AGCAATGTCA GCCTCTGCTA 
GGGCAGCTGC CGCTAGGGCA GCCTATGCAG GTCCACTGGT CTGGGGAGCC AGGTCACTCT 
CAGCTACTCC CGCCGCTCGG GCATCCCTTC CTGCCCGCGC AGCAGCTGCC GCCCGGGCAG 
CCTCTGCTGC CCGCGCAGTC GCTGCTGGCC GGTCAGCCTC TGCCGCCCCC AGCAGGGCCC 
ATCTTAGACC CCCCAGCCCC GAGATCCAGG TTGCTGACCC GCCTACTCCG CGGCCTCCTC 
CGCGGCCGAC TGCCTGGCCT GACAAGTACG AGCGGGGCCG AAGCTGCTGC AGGTACGAGG 
CATCGTCTGG CATCTGCGAG ATCGAGTCCT CCAGTGATGA GTCGGAAGAA GGGGCCACCG 
GCTGCTTCCA GTGGCTTCTG CGGCGAAACC GCCGCCCTGG CCTGCCCCGG AGCCACACGG 
TCGGGAGCAA CCCAGTCCGC AACTTCTTCA CCCGAGCCTT CGGAAGCTGC TTCGGTCTAT 
CCGAGTGTAC CCGATCACGA TCCCTCAGCC CCGGGAAGGC CAAGGATCCT ATGGAGGAGA 
GGCGCAAACA GATGCGCAAA GAAGCCATTG AGATGCGAGA GCAGAAGCGC GCAGATAAGA 
AACGCAGCAA GCTCATCGAC AAGCAACTGG AGGAGGAGAA GATGGACTAC ATGTGTACAC 
ACCGCCTGCT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_010309.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034439.2 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, mRNA.

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CloneID	OMu21786
Clone ID Related Accession (Same CDS sequence)	NM_010309.4
Accession Version	NM_010309.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform XLas
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10, AK132636.1 and BU516278.1. On Jun 20, 2015 this sequence version replaced NM_010309.3. Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, compared to variant 7. This variant includes two overlapping open reading frames encoding distinct isoforms XLas and Alex, respectively. This RefSeq represents the isoform XLas (also known as alpha sXXL), which is a neuroendocrine-specific G-protein alpha subunit. It has a distinct and longer N-terminus, compared to isoform GNASL. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGTGC TGGAGAGTCT GGCAAAAGCA CCATTGTGAA GCAGATGAGG 
ATCCTGCATG TTAATGGGTT TAACGGAGAG GGCGGCGAAG AGGACCCGCA GGCTGCAAGG 
AGCAACAGCG ATGGTGAGAA GGCCACTAAA GTGCAGGACA TCAAAAACAA CCTGAAGGAG 
GCCATTGAAA CCATTGTGGC CGCCATGAGC AACCTGGTGC CCCCTGTGGA GCTGGCCAAC 
CCTGAGAACC AGTTCAGAGT GGACTACATT CTGAGCGTGA TGAACGTGCC GAACTTTGAC 
TTCCCACCTG AATTCTATGA GCATGCCAAG GCTCTGTGGG AGGATGAGGG AGTGCGTGCC 
TGCTACGAGC GCTCCAATGA GTACCAGCTG ATTGACTGTG CCCAGTACTT CCTGGACAAG 
ATTGATGTGA TCAAGCAGGC CGACTACGTG CCAAGTGACC AGGACCTGCT TCGCTGCCGT 
GTCCTGACCT CTGGAATCTT TGAGACCAAG TTCCAGGTGG ACAAAGTCAA CTTCCACATG 
TTCGATGTGG GCGGCCAGCG CGATGAGCGC CGCAAGTGGA TCCAGTGCTT CAATGATGTG 
ACTGCCATCA TCTTCGTGGT GGCCAGCAGC AGCTACAACA TGGTCATTCG GGAGGACAAC 
CAGACTAACC GCCTGCAGGA GGCTCTGAAC CTCTTCAAGA GCATCTGGAA CAACAGATGG 
CTGCGCACCA TCTCTGTGAT TCTCTTCCTC AACAAGCAAG ACCTGCTTGC TGAGAAAGTC 
CTCGCTGGCA AATCGAAGAT TGAGGACTAC TTTCCAGAGT TCGCTCGCTA CACCACTCCT 
GAGGATGCGA CTCCCGAGCC GGGAGAGGAC CCACGCGTGA CCCGGGCCAA GTACTTCATT 
CGGGATGAGT TTCTGAGAAT CAGCACTGCT AGTGGAGATG GGCGCCACTA CTGCTACCCT 
CACTTTACCT GCGCCGTGGA CACTGAGAAC ATCCGCCGTG TCTTCAACGA CTGCCGTGAC 
ATCATCCAGC GCATGCATCT CCGCCAATAC GAGCTGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034439.2
CDS	4..3405
Translation	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSECAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPEAMDAKPAPAAQAVSTGSDAGAPTDSAMLTDSQSDAGEDGTAPGTPSDLQSDPEELEEAPAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVPATTAESASGAAPVTQVEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_010309.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010309.4

ORF Insert Sequence:

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGTGC TGGAGAGTCT GGCAAAAGCA CCATTGTGAA GCAGATGAGG 
ATCCTGCATG TTAATGGGTT TAACGGAGAG GGCGGCGAAG AGGACCCGCA GGCTGCAAGG 
AGCAACAGCG ATGGTGAGAA GGCCACTAAA GTGCAGGACA TCAAAAACAA CCTGAAGGAG 
GCCATTGAAA CCATTGTGGC CGCCATGAGC AACCTGGTGC CCCCTGTGGA GCTGGCCAAC 
CCTGAGAACC AGTTCAGAGT GGACTACATT CTGAGCGTGA TGAACGTGCC GAACTTTGAC 
TTCCCACCTG AATTCTATGA GCATGCCAAG GCTCTGTGGG AGGATGAGGG AGTGCGTGCC 
TGCTACGAGC GCTCCAATGA GTACCAGCTG ATTGACTGTG CCCAGTACTT CCTGGACAAG 
ATTGATGTGA TCAAGCAGGC CGACTACGTG CCAAGTGACC AGGACCTGCT TCGCTGCCGT 
GTCCTGACCT CTGGAATCTT TGAGACCAAG TTCCAGGTGG ACAAAGTCAA CTTCCACATG 
TTCGATGTGG GCGGCCAGCG CGATGAGCGC CGCAAGTGGA TCCAGTGCTT CAATGATGTG 
ACTGCCATCA TCTTCGTGGT GGCCAGCAGC AGCTACAACA TGGTCATTCG GGAGGACAAC 
CAGACTAACC GCCTGCAGGA GGCTCTGAAC CTCTTCAAGA GCATCTGGAA CAACAGATGG 
CTGCGCACCA TCTCTGTGAT TCTCTTCCTC AACAAGCAAG ACCTGCTTGC TGAGAAAGTC 
CTCGCTGGCA AATCGAAGAT TGAGGACTAC TTTCCAGAGT TCGCTCGCTA CACCACTCCT 
GAGGATGCGA CTCCCGAGCC GGGAGAGGAC CCACGCGTGA CCCGGGCCAA GTACTTCATT 
CGGGATGAGT TTCTGAGAAT CAGCACTGCT AGTGGAGATG GGCGCCACTA CTGCTACCCT 
CACTTTACCT GCGCCGTGGA CACTGAGAAC ATCCGCCGTG TCTTCAACGA CTGCCGTGAC 
ATCATCCAGC GCATGCATCT CCGCCAATAC GAGCTGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001077507.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001070975.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 5, mRNA.

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CloneID	OMu21797
Clone ID Related Accession (Same CDS sequence)	NM_001077507.2
Accession Version	NM_001077507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10, AK030489.1, AF116268.1 and BU516278.1. On Jun 25, 2015 this sequence version replaced NM_001077507.1. Transcript Variant: This variant (5, also known as GNASXL) is paternally expressed. It has an alternate 5' exon, lacks an internal exon and uses an alternate splice site in the coding region, compared to variant 7. It encodes isoform f (also known as XLas), which has a longer and distinct N-terminus and lacks an internal segment, compared to isoform GNASL. Isoform f is the neuroendocrine-specific G-protein alpha subunit. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGTGC TGGAGAGTCT GGCAAAAGCA CCATTGTGAA GCAGATGAGG 
ATCCTGCATG TTAATGGGTT TAACGGAGAT AGTGAGAAGG CCACTAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCTGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCGA ACTTTGACTT CCCACCTGAA TTCTATGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TGCGTGCCTG CTACGAGCGC TCCAATGAGT ACCAGCTGAT TGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGTGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAGCGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATTCGGG AGGACAACCA GACTAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGCACCATC TCTGTGATTC TCTTCCTCAA CAAGCAAGAC 
CTGCTTGCTG AGAAAGTCCT CGCTGGCAAA TCGAAGATTG AGGACTACTT TCCAGAGTTC 
GCTCGCTACA CCACTCCTGA GGATGCGACT CCCGAGCCGG GAGAGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATTCG GGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGG 
CGCCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTCC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070975.1
CDS	4..3363
Translation	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSECAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPEAMDAKPAPAAQAVSTGSDAGAPTDSAMLTDSQSDAGEDGTAPGTPSDLQSDPEELEEAPAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVPATTAESASGAAPVTQVEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001077507.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077507.2

ORF Insert Sequence:

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGTGC TGGAGAGTCT GGCAAAAGCA CCATTGTGAA GCAGATGAGG 
ATCCTGCATG TTAATGGGTT TAACGGAGAT AGTGAGAAGG CCACTAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCTGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCGA ACTTTGACTT CCCACCTGAA TTCTATGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TGCGTGCCTG CTACGAGCGC TCCAATGAGT ACCAGCTGAT TGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGTGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAGCGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TTCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATTCGGG AGGACAACCA GACTAACCGC CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGCACCATC TCTGTGATTC TCTTCCTCAA CAAGCAAGAC 
CTGCTTGCTG AGAAAGTCCT CGCTGGCAAA TCGAAGATTG AGGACTACTT TCCAGAGTTC 
GCTCGCTACA CCACTCCTGA GGATGCGACT CCCGAGCCGG GAGAGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATTCG GGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGG 
CGCCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTCC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498780.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498843.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X11, mRNA.

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CloneID	OMu30033
Clone ID Related Accession (Same CDS sequence)	XM_006498780.1
Accession Version	XM_006498780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGTGC TGGAGAGTCT GGCAAAAGCA CCATTGTGAA GCAGATGAGG 
ATCCTGCATG TTAATGGGTT TAACGGAGAT GAGAAGGCCA CTAAAGTGCA GGACATCAAA 
AACAACCTGA AGGAGGCCAT TGAAACCATT GTGGCCGCCA TGAGCAACCT GGTGCCCCCT 
GTGGAGCTGG CCAACCCTGA GAACCAGTTC AGAGTGGACT ACATTCTGAG CGTGATGAAC 
GTGCCGAACT TTGACTTCCC ACCTGAATTC TATGAGCATG CCAAGGCTCT GTGGGAGGAT 
GAGGGAGTGC GTGCCTGCTA CGAGCGCTCC AATGAGTACC AGCTGATTGA CTGTGCCCAG 
TACTTCCTGG ACAAGATTGA TGTGATCAAG CAGGCCGACT ACGTGCCAAG TGACCAGGAC 
CTGCTTCGCT GCCGTGTCCT GACCTCTGGA ATCTTTGAGA CCAAGTTCCA GGTGGACAAA 
GTCAACTTCC ACATGTTCGA TGTGGGCGGC CAGCGCGATG AGCGCCGCAA GTGGATCCAG 
TGCTTCAATG ATGTGACTGC CATCATCTTC GTGGTGGCCA GCAGCAGCTA CAACATGGTC 
ATTCGGGAGG ACAACCAGAC TAACCGCCTG CAGGAGGCTC TGAACCTCTT CAAGAGCATC 
TGGAACAACA GATGGCTGCG CACCATCTCT GTGATTCTCT TCCTCAACAA GCAAGACCTG 
CTTGCTGAGA AAGTCCTCGC TGGCAAATCG AAGATTGAGG ACTACTTTCC AGAGTTCGCT 
CGCTACACCA CTCCTGAGGA TGCGACTCCC GAGCCGGGAG AGGACCCACG CGTGACCCGG 
GCCAAGTACT TCATTCGGGA TGAGTTTCTG AGAATCAGCA CTGCTAGTGG AGATGGGCGC 
CACTACTGCT ACCCTCACTT TACCTGCGCC GTGGACACTG AGAACATCCG CCGTGTCTTC 
AACGACTGCC GTGACATCAT CCAGCGCATG CATCTCCGCC AATACGAGCT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498843.1
CDS	513..3869
Translation	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSECAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPEAMDAKPAPAAQAVSTGSDAGAPTDSAMLTDSQSDAGEDGTAPGTPSDLQSDPEELEEAPAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVPATTAESASGAAPVTQVEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	XM_006498780.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498780.1

ORF Insert Sequence:

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGTGC TGGAGAGTCT GGCAAAAGCA CCATTGTGAA GCAGATGAGG 
ATCCTGCATG TTAATGGGTT TAACGGAGAT GAGAAGGCCA CTAAAGTGCA GGACATCAAA 
AACAACCTGA AGGAGGCCAT TGAAACCATT GTGGCCGCCA TGAGCAACCT GGTGCCCCCT 
GTGGAGCTGG CCAACCCTGA GAACCAGTTC AGAGTGGACT ACATTCTGAG CGTGATGAAC 
GTGCCGAACT TTGACTTCCC ACCTGAATTC TATGAGCATG CCAAGGCTCT GTGGGAGGAT 
GAGGGAGTGC GTGCCTGCTA CGAGCGCTCC AATGAGTACC AGCTGATTGA CTGTGCCCAG 
TACTTCCTGG ACAAGATTGA TGTGATCAAG CAGGCCGACT ACGTGCCAAG TGACCAGGAC 
CTGCTTCGCT GCCGTGTCCT GACCTCTGGA ATCTTTGAGA CCAAGTTCCA GGTGGACAAA 
GTCAACTTCC ACATGTTCGA TGTGGGCGGC CAGCGCGATG AGCGCCGCAA GTGGATCCAG 
TGCTTCAATG ATGTGACTGC CATCATCTTC GTGGTGGCCA GCAGCAGCTA CAACATGGTC 
ATTCGGGAGG ACAACCAGAC TAACCGCCTG CAGGAGGCTC TGAACCTCTT CAAGAGCATC 
TGGAACAACA GATGGCTGCG CACCATCTCT GTGATTCTCT TCCTCAACAA GCAAGACCTG 
CTTGCTGAGA AAGTCCTCGC TGGCAAATCG AAGATTGAGG ACTACTTTCC AGAGTTCGCT 
CGCTACACCA CTCCTGAGGA TGCGACTCCC GAGCCGGGAG AGGACCCACG CGTGACCCGG 
GCCAAGTACT TCATTCGGGA TGAGTTTCTG AGAATCAGCA CTGCTAGTGG AGATGGGCGC 
CACTACTGCT ACCCTCACTT TACCTGCGCC GTGGACACTG AGAACATCCG CCGTGTCTTC 
AACGACTGCC GTGACATCAT CCAGCGCATG CATCTCCGCC AATACGAGCT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_022000.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_068840.2 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 3, mRNA.

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CloneID	OMu21719
Clone ID Related Accession (Same CDS sequence)	NM_022000.3 , XM_006498779.3 , NM_019690.3 , XM_006498779.4
Accession Version	NM_022000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	protein SCG6 (secretogranin VI) isoform SCG6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK039035.1, AJ010163.1, AK132636.1 and BU516278.1. On Jun 20, 2015 this sequence version replaced NM_022000.2. Transcript Variant: This variant (3) is maternally expressed and has alternate 5' exons, compared to variant 7. Variants 3 and 4 both encode isoform secretogranin VI (SCG6, also known as NESP55), which localizes to large secretory vesicles of endocrine cells and neurons. The coding regions of variants 3 and 4 do not overlap the coding regions used by other transcripts; thus SCG6 has no similarity to isoforms of the G-protein alpha subunit. This variant has an antisense transcript NESPAS. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 NMD candidate :: PMID: 15798190 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068840.2
CDS	260..1021
Translation	MDRRSRAQQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAAQRRSFLNAHHRSAAAAAAAQVLPESSESESDHEHEEVEPELARPECLEYDQDDYETETDSETEPESDIESETEIETEPETEPETEPETEPEDERGPRGATFNQSLTQRLHALKLQSADASPRRAQPTTQEPESASEGEEPQRGPLDQDPRDPEEEPEERKEENRQPRRCKTRRPARRRDQSPESPPRKGPIPIRRH

Target ORF information:

RefSeq Version	NM_022000.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022000.3

ORF Insert Sequence:

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_019690.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062664.2 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 4, mRNA.

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CloneID	OMu21719
Clone ID Related Accession (Same CDS sequence)	NM_022000.3 , XM_006498779.3 , NM_019690.3 , XM_006498779.4
Accession Version	NM_019690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	protein SCG6 (secretogranin VI) isoform SCG6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB632851.1, CF730992.1, AJ010163.1, BY718446.1, AL593857.10, BY600941.1, BX636499.1 and AV353621.1. On Jun 20, 2015 this sequence version replaced NM_019690.2. Transcript Variant: This variant (4) is maternally expressed. It lacks several 3' exons and has alternate 5' and 3' exons, compared to variant 7. Variants 3 and 4 both encode isoform secretogranin VI (SCG6, also known as NESP55), which localizes to large secretory vesicles of endocrine cells and neurons. The coding regions of variants 3 and 4 do not overlap the coding regions used by other transcripts; thus SCG6 has no similarity to isoforms of the G-protein alpha subunit. This variant has an antisense transcript NESPAS. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 NMD candidate :: PMID: 15798190 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062664.2
CDS	260..1021
Translation	MDRRSRAQQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAAQRRSFLNAHHRSAAAAAAAQVLPESSESESDHEHEEVEPELARPECLEYDQDDYETETDSETEPESDIESETEIETEPETEPETEPETEPEDERGPRGATFNQSLTQRLHALKLQSADASPRRAQPTTQEPESASEGEEPQRGPLDQDPRDPEEEPEERKEENRQPRRCKTRRPARRRDQSPESPPRKGPIPIRRH

Target ORF information:

RefSeq Version	NM_019690.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019690.3

ORF Insert Sequence:

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_201617.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_963911.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6, mRNA.

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CloneID	OMu21738
Clone ID Related Accession (Same CDS sequence)	NM_201617.2
Accession Version	NM_201617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform XXLb1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10 and AK030489.1. On Jun 29, 2015 this sequence version replaced NM_201617.1. Transcript Variant: This variant (6) is paternally expressed. Its 5' exon overlaps with variant 1 and none of its sequence overlaps with variant 7. This variant encodes isoform XXLb1, which has a distinct and shorter C-terminus, compared to isoform XLas. This variant is based on data in PMID:15148396. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 ##RefSeq-Attributes-END##

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGGAG AAAAGTGGTG CCGAGCGACA CTGAGGGTCG TTACCGGCCG 
GAGGCCAGCG CCAGCGCCAG CGATCGTAGG CTGGACAGGC GGGGGCGTGA GGTGAGCCCA 
GAACTGCTGG GGTGGGCCCT TCGGGGCTCC CCCGGCTCGA TTGTTCGTGA CCGCGGTGGG 
CTAGGGCCAT CCGGGTGCGC GCCCCCGCCT CGCCTGGCAC GGCTGCTTCG ACTCAGACAG 
CTTGTTGTTG GTGTGTGTTG GTGTCCATTT TCTGTGTTCG CCTGTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963911.1
CDS	4..2634
Translation	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSECAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPEAMDAKPAPAAQAVSTGSDAGAPTDSAMLTDSQSDAGEDGTAPGTPSDLQSDPEELEEAPAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVPATTAESASGAAPVTQVEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGRKVVPSDTEGRYRPEASASASDRRLDRRGREVSPELLGWALRGSPGSIVRDRGGLGPSGCAPPPRLARLLRLRQLVVGVCWCPFSVFACA

Target ORF information:

RefSeq Version	NM_201617.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201617.2

ORF Insert Sequence:

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGGAG AAAAGTGGTG CCGAGCGACA CTGAGGGTCG TTACCGGCCG 
GAGGCCAGCG CCAGCGCCAG CGATCGTAGG CTGGACAGGC GGGGGCGTGA GGTGAGCCCA 
GAACTGCTGG GGTGGGCCCT TCGGGGCTCC CCCGGCTCGA TTGTTCGTGA CCGCGGTGGG 
CTAGGGCCAT CCGGGTGCGC GCCCCCGCCT CGCCTGGCAC GGCTGCTTCG ACTCAGACAG 
CTTGTTGTTG GTGTGTGTTG GTGTCCATTT TCTGTGTTCG CCTGTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001310085.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297014.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OMu67835
Clone ID Related Accession (Same CDS sequence)	NM_001310085.1
Accession Version	NM_001310085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	protein GNAS isoform XXLb2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL593857.10, AK030489.1, AK132636.1 and BU516278.1. Transcript Variant: This variant (11) is paternally expressed and includes an alternate 5' exon structure, compared to variant 7. This variant is reported in PMID:15148396. It encodes isoform XXLb2, which has a distinct and shorter C-terminus, compared to isoform XLas. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 10097123, 11056047 NMD candidate :: PMID: 15148396 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGGAG AAAAGTGGTG CCGAGCGACA CTGAGGGTCG TTACCGGCCG 
GAGGCCAGCG CCAGCGCCAG CGATCGTAGG CTGGACAGGC GGGGGCGTGA GGTGCTGGAG 
AGTCTGGCAA AAGCACCATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297014.1
CDS	4..2487
Translation	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSECAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPEAMDAKPAPAAQAVSTGSDAGAPTDSAMLTDSQSDAGEDGTAPGTPSDLQSDPEELEEAPAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVPATTAESASGAAPVTQVEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGRKVVPSDTEGRYRPEASASASDRRLDRRGREVLESLAKAPL

Target ORF information:

RefSeq Version	NM_001310085.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310085.1

ORF Insert Sequence:

ATGGGCATGT TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG AGCAGCCCGA GCAAGAACCT TTGGAAGCCC CAGGGGCAGC TGCCCCCGGT 
GCTGGGGCTG GCCCAGCCGA AGAAATGGCG ACCGAACCGG ACTCCGAACC GTCTAACAAT 
GAGCCCGTCC CCGACGAGAC TGGCAGTGAG ATCAGTGGAC CCCCAGAAGA CTCCAAATCT 
GACATCCAAA GCCCCTGCCA GGCCTTCGAG GAAGTCCGAG TGGGTGGAGA CTACAGCCCA 
CCTCCGGAGG AAGCCATGCC ATTCGAGACA CAACAGCCCA GCCTGGGAGA TTTCTGGCCC 
ACCCTGGAGC AGCCAGGACC ATCTGGGACC CCATCAGGCC TCCAAGCCTT CAACCCAGCG 
ATTTTGGAGC CCGGGACCCC CACTGGCGCG AGCCCAGGCC TGGGAGCCTA TACCCCCCCA 
CCAGAAGAAG CTATGCCATT TGAGTTCAAC GAGCCTGCCC AGGGAGACCA TAGCCAGCCT 
CCCTTGCAAG TCCCAGACCT TGCGCCAGGA GGTCCGGAAG CATTGGTCCC CAGAGCTCTT 
CCCGCGGAGC CCGGGAACAT CAGATTTGAA AACGCTGGCT TCCGAGAAGA CTACAGCCCT 
CCCCCTGAAG AATCTGTGCC ATTTCAGGTC GGTGGAGAAG AATTCGGGGG CGATAGCCCA 
CCCCCAGGAC TCCCGCGAGT CATCCCACAA ATCGGCATTG GCGGCGAGTT CCCGACAGTC 
GCGGTCCCGA GTGCGCTCTG CCTCGCTCCC GCCGAGAACG CGCCTCCCCT CTGGGTCCGA 
GGCGCCATTG ACAGACCATT CCGCGAGGCT GTCAGATCTC CTCCTAACTT CGCATGCGAC 
AGCCCCCCGA TGGAGATCAC CAGACCCCTG CTTGAGATTG GCAGAGCCTC CATTGGGGTC 
GACGACGACA CCGCTGTCAA TATGGACAGC CCCCCAATCG CAAGTGATGG CCCGCCCATC 
GAAGTCTCGG GAGCCCCAGA TAAGAGCGAG TGCGCAGAGA GACCCCCAGT TGAGCGAGAA 
GCAGCCGAGA TGGAAGGAAG CCCTACCACC GCCACTGCGG TGGAAGGAAA AGTCCCCTCT 
CCGGAGAGAG GGGACGGATC TTCCACCCAG CCTGAAGCAA TGGATGCCAA GCCAGCCCCT 
GCTGCCCAAG CCGTCTCTAC CGGATCTGAT GCTGGAGCTC CTACGGATTC CGCGATGCTC 
ACAGATAGCC AGAGCGATGC CGGAGAAGAC GGGACAGCCC CAGGAACGCC TTCAGATCTC 
CAGTCGGATC CTGAAGAACT CGAAGAAGCC CCAGCTGTCC GCGCCGATCC TGACGGAGGG 
GCAGCCCCAG TCGCCCCAGC CACTCCTGCC GAGTCCGAGT CTGAAGGCAG CAGAGATCCA 
GCCGCCGAGC CAGCCTCCGA GGCAGTCCCT GCCACCACGG CCGAGTCTGC CTCCGGGGCA 
GCCCCTGTCA CCCAGGTGGA GCCCGCAGCC GCGGCAGTCT CTGCCACCCT GGCGGAGCCT 
GCCGCCCGGG CAGCCCCTAT CACCCCCAAG GAGCCCACTA CCCGGGCAGT CCCCTCTGCT 
AGAGCCCATC CGGCCGCTGG AGCAGTCCCT GGCGCCCCAG CAATGTCAGC CTCTGCTAGG 
GCAGCTGCCG CTAGGGCAGC CTATGCAGGT CCACTGGTCT GGGGAGCCAG GTCACTCTCA 
GCTACTCCCG CCGCTCGGGC ATCCCTTCCT GCCCGCGCAG CAGCTGCCGC CCGGGCAGCC 
TCTGCTGCCC GCGCAGTCGC TGCTGGCCGG TCAGCCTCTG CCGCCCCCAG CAGGGCCCAT 
CTTAGACCCC CCAGCCCCGA GATCCAGGTT GCTGACCCGC CTACTCCGCG GCCTCCTCCG 
CGGCCGACTG CCTGGCCTGA CAAGTACGAG CGGGGCCGAA GCTGCTGCAG GTACGAGGCA 
TCGTCTGGCA TCTGCGAGAT CGAGTCCTCC AGTGATGAGT CGGAAGAAGG GGCCACCGGC 
TGCTTCCAGT GGCTTCTGCG GCGAAACCGC CGCCCTGGCC TGCCCCGGAG CCACACGGTC 
GGGAGCAACC CAGTCCGCAA CTTCTTCACC CGAGCCTTCG GAAGCTGCTT CGGTCTATCC 
GAGTGTACCC GATCACGATC CCTCAGCCCC GGGAAGGCCA AGGATCCTAT GGAGGAGAGG 
CGCAAACAGA TGCGCAAAGA AGCCATTGAG ATGCGAGAGC AGAAGCGCGC AGATAAGAAA 
CGCAGCAAGC TCATCGACAA GCAACTGGAG GAGGAGAAGA TGGACTACAT GTGTACACAC 
CGCCTGCTGC TTCTAGGGAG AAAAGTGGTG CCGAGCGACA CTGAGGGTCG TTACCGGCCG 
GAGGCCAGCG CCAGCGCCAG CGATCGTAGG CTGGACAGGC GGGGGCGTGA GGTGCTGGAG 
AGTCTGGCAA AAGCACCATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( XM_006498779.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498842.1 Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X8, mRNA.

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CloneID	OMu21719
Clone ID Related Accession (Same CDS sequence)	NM_022000.3 , XM_006498779.3 , NM_019690.3 , XM_006498779.4
Accession Version	XM_006498779.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SCG6 (secretogranin VI) isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498842.1
CDS	274..1035
Translation	MDRRSRAQQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAAQRRSFLNAHHRSAAAAAAAQVLPESSESESDHEHEEVEPELARPECLEYDQDDYETETDSETEPESDIESETEIETEPETEPETEPETEPEDERGPRGATFNQSLTQRLHALKLQSADASPRRAQPTTQEPESASEGEEPQRGPLDQDPRDPEEEPEERKEENRQPRRCKTRRPARRRDQSPESPPRKGPIPIRRH

Target ORF information:

RefSeq Version	XM_006498779.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498779.3

ORF Insert Sequence:

ATGGATCGCA GGTCCCGGGC TCAGCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCCACC GCTCTCCTCT GGCTCTCCTG CTCCATTGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGTGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCT 
GAGTCCTCTG AATCTGAGTC TGATCACGAG CACGAGGAGG TTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCGAATC CGAGACCGAA ATCGAGACCG AGCCAGAGAC CGAGCCAGAA 
ACCGAGCCAG AGACCGAGCC AGAGGACGAG CGCGGCCCCC GGGGTGCCAC CTTCAACCAG 
TCACTCACTC AGCGTCTGCA CGCTCTGAAG TTGCAGAGCG CCGACGCCTC CCCGAGACGT 
GCGCAGCCCA CCACTCAGGA GCCTGAGAGC GCAAGCGAGG GGGAGGAGCC CCAGCGAGGG 
CCCTTAGATC AGGATCCTCG GGACCCCGAG GAGGAGCCAG AGGAGCGCAA GGAGGAAAAC 
AGGCAGCCCC GCCGCTGCAA GACCAGGAGG CCAGCCCGCC GTCGCGACCA GTCCCCGGAG 
TCCCCTCCCA GAAAGGGGCC CATCCCCATC CGGCGTCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interference with Gs
Journal of the American Society of Nephrology : JASN28(12)3479-3489(2017 Dec)
Lachmann P,Hickmann L,Steglich A,Al-Mekhlafi M,Gerlach M,Jetschin N,Jahn S,Hamann B,Wnuk M,Madsen K,Djonov V,Chen M,Weinstein LS,Hohenstein B,Hugo CPM,Todorov VT

Internalized TSH receptors en route to the TGN induce local G
Nature communications8(1)443(2017 09)
Godbole A,Lyga S,Lohse MJ,Calebiro D

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Gnas cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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