Gli3 cDNA ORF clone, Mus musculus(house mouse)

The following Gli3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu78425 XM_017315389.1
Latest version!
Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu21819 NM_008130.3
Latest version!
Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu48321 XM_006516552.3
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Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
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OMu21819 NM_008130.2 Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu78425
    Clone ID Related Accession (Same CDS sequence) XM_017315389.1
    Accession Version XM_017315389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTCTTGTT CTCACTTCCT AAGGACAGTG GGTAATAAGA CATCTCAGCC AAGCCCTGGT 
    GGTCAGTCTT CATGCAGCAG CCAACAGTCC CCCATCAGCA ACTATTCCAA CAGTGGGCTC
    GAGCTTCCTC TGACTGATGG AGGTAGTGTA GCAGACCTCA GTGCCATCGA TGAAACCCCA
    ATCATGGACT CGACCATTTC CACGGCAACC ACAGCCCTTG CTTTGCAAGC CAGGAGAAAC
    CCGGCAGGGA CCAAATGGAT GGAGCACATC AAACTTGAAA GGCTAAAACA AGTGAATGGA
    ATGTTTCCAA GACTGAACCC TATTCTACCC TCCAAAGCCC CTGCGGTATC TCCTCTCATA
    GGAAATGGCA CACAGTCCAA TAACAACTAT AGCTCGGGTG GGCCCGGGAC CCTCCTCCCG
    AGCAGAAGTG ACCTGTCAGG TGTAGACTTC ACAGTGCTGA ACACACTCAA CAGGAGAGAC
    AGCAATACCA GCACTATCAG CTCTGCCTAC CTGAGCAGCC GCAGATCCTC GGGCATCTCC
    CCCTGCTTTT CCAGCCGCAG GTCCAGCGAG GCGTCCCAGG CTGAAGGGCG ACCCCAGAAT
    GTGAGTGTGG CTGACTCCTA CGATCCCATC TCCACAGATG CTTCAAGGAG GTCCAGCGAG
    GCCAGCCAGG GTGACGGGCT GCCCAGTCTG CTCAGCCTCA CACCCGTGCA GCAGTACCGC
    CTTAAGGCCA AGTATGCAGC TGCCACAGGT GGCCCACCAC CCACACCTCT GCCCCACATG
    GAGAGGCTGA GCCTGAAGAC CAAGATGGCC TTGCTAGGGG AAGGGAGGGA CTCTGGGGTG
    ACCCTGCCTC CAGTCCATCC TCCTCGAAGA TGCAGTGATG GGGGAGGTCA CACATACAGA
    GGGCGTCACC TGATGCCTCA TGATGCACTA GCGAACAGTG TGAGGAGAGC CAGCGACCCT
    GTGAGGACAG TCTCGGAGAA CATGTCACTT GCCAGGGTTC AACGCTTCAG CAGCCTCAAT
    AGCTTTAATC CTCCAAATTT GCCTCCATCG GTGGAAAAGC GTAGCTTAGT GCTGCAAAAT
    TATACCCGGC AGGAGAGCAG CCAACCCCGG TACTTCCAGG CATCCCCTTG CCCTCCCAGC
    ATCACAGAGA ATGTTGCCCT GGAGGCTCTG ACCATGGATG CTGATGCTAA CTTGAATGAT
    GAAGACCTCC TGCCAGATGA TGTGGTACAG TATTTAAATT CCCAGAACCA AACAGGGTAT
    GGGCAGCAGT TGCAGAGTGG CATCTCTGAA GACAGCAAAG TAGCCCATGA GCCAGAGGAC
    TTGGACTTAG CAGGGCTGCC AGACAGTCAT GTTGGCCAGG AGTACCCAGC TCTGGAGCAG
    CCCTGCTCTG AGGGCAGTAA AACTGATTTG CCCATCCAGT GGAACGAGGT CAGTTCTGGA
    ACTTCTGATC TGTCATCCTC CAAGCTGAAG TGTGGTCAGC AGCGCCCTAG CGCACAGCAG
    CCTCGGGGTT TTGGGCTATA CAACAATATG GTGGTACACC CACATAATCT GTGGAAGGTT
    GGCACTGGCC CAGCTGGGGG ATATCAGACC CTCGGGGAGA ATAGCAGTAC CTACAATGGC
    CCAGAGCACT TTGCAATCCA CAGTGGGGAT GGACTTGGCA CCAATGGAAA TACTTTCCAT
    GAACAGCCCT TTAAGACCCA GCAGTATGGG AGCCAGCTCA ACAGGCAGCC ACTGACTTCC
    AGTGCTCTAG ATCATGCCTG TGGTACAGGG ATTCAAGGGT CCAAGCTAAA AGGCAACAGC
    TTGCAAGAGA ATGGGGGTTT GCTAGATTTC AGCCTGTCCG TGGCACCAAA TGAGTTAGCT
    GGCAACACAG TGAATGGCAT GCAAACCCAA GATCAAATGG GACAGGGATA CATTGCCCAT
    CAGCTACTCA GTGGCAGCAT GCAACACCAG GGGCCCAGCC GCCCTGGTCA ACAGGTACTA
    GGGCAGGTTG GTGCTACCTC ACATATCAAC ATCTATCAAG GGACAGAGAG CTGCCTACCA
    GGGACTCAGG ACAACAGCAG CCAGCCATCA AGCATGGCAG CTATCAGGGG CTACCAGCCC
    TGTGCCAGCT ATGGGGGTAA CAGGCGTCAG GCAATGCCAA GGGGCAACCT CACTCTGCAA
    CAAGGACAGC TCAGTGACAT GAGTCAGAGC AGCAGGGTGA ACAGCATCAA AATGGAGGCA
    CAAGGTCAGT CCCAGCAGCT CTGCTCTACC GTGCAGAATT ATTCCGGTCA GTTCTATGAC
    CAAACCATGG GCTTCAGTCA GCAAGACAGG AAAGCTGGCT CGTTCTCCCT CTCAGATGCC
    AACTGCCTGC TCCAAGGGAA TGGCACTGAA AACTCTGAGT TACTCTCCCC AGGTGCTAAC
    CAGGTAACAA GCACAGTTGA CAGCTTTGAG AGTCATGACC TAGAAGGTGT GCAGATTGAT
    TTTGATGCCA TCATAGATGA TGGGGACCAT ACCAGCCTAA TGTCAGGGGC CTTGAGCCCA
    AGTATTATTC AGAACCTTTC CCACAGCTCC TCCCGTCTCA CCACTCCGCG GGCATCCCTC
    CCATTCCCAT CCCTATCCAT GGGCACCACC AACATGGCTA TCGGGGATAT GAGTTCTTTG
    CTGACCTCCC TTGCAGAAGA AAGCAAGTTC CTTGCAGTTA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017170878.1
    CDS49..2736
    Translation

    Target ORF information:

    RefSeq Version XM_017315389.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTCTTGTT CTCACTTCCT AAGGACAGTG GGTAATAAGA CATCTCAGCC AAGCCCTGGT 
    GGTCAGTCTT CATGCAGCAG CCAACAGTCC CCCATCAGCA ACTATTCCAA CAGTGGGCTC
    GAGCTTCCTC TGACTGATGG AGGTAGTGTA GCAGACCTCA GTGCCATCGA TGAAACCCCA
    ATCATGGACT CGACCATTTC CACGGCAACC ACAGCCCTTG CTTTGCAAGC CAGGAGAAAC
    CCGGCAGGGA CCAAATGGAT GGAGCACATC AAACTTGAAA GGCTAAAACA AGTGAATGGA
    ATGTTTCCAA GACTGAACCC TATTCTACCC TCCAAAGCCC CTGCGGTATC TCCTCTCATA
    GGAAATGGCA CACAGTCCAA TAACAACTAT AGCTCGGGTG GGCCCGGGAC CCTCCTCCCG
    AGCAGAAGTG ACCTGTCAGG TGTAGACTTC ACAGTGCTGA ACACACTCAA CAGGAGAGAC
    AGCAATACCA GCACTATCAG CTCTGCCTAC CTGAGCAGCC GCAGATCCTC GGGCATCTCC
    CCCTGCTTTT CCAGCCGCAG GTCCAGCGAG GCGTCCCAGG CTGAAGGGCG ACCCCAGAAT
    GTGAGTGTGG CTGACTCCTA CGATCCCATC TCCACAGATG CTTCAAGGAG GTCCAGCGAG
    GCCAGCCAGG GTGACGGGCT GCCCAGTCTG CTCAGCCTCA CACCCGTGCA GCAGTACCGC
    CTTAAGGCCA AGTATGCAGC TGCCACAGGT GGCCCACCAC CCACACCTCT GCCCCACATG
    GAGAGGCTGA GCCTGAAGAC CAAGATGGCC TTGCTAGGGG AAGGGAGGGA CTCTGGGGTG
    ACCCTGCCTC CAGTCCATCC TCCTCGAAGA TGCAGTGATG GGGGAGGTCA CACATACAGA
    GGGCGTCACC TGATGCCTCA TGATGCACTA GCGAACAGTG TGAGGAGAGC CAGCGACCCT
    GTGAGGACAG TCTCGGAGAA CATGTCACTT GCCAGGGTTC AACGCTTCAG CAGCCTCAAT
    AGCTTTAATC CTCCAAATTT GCCTCCATCG GTGGAAAAGC GTAGCTTAGT GCTGCAAAAT
    TATACCCGGC AGGAGAGCAG CCAACCCCGG TACTTCCAGG CATCCCCTTG CCCTCCCAGC
    ATCACAGAGA ATGTTGCCCT GGAGGCTCTG ACCATGGATG CTGATGCTAA CTTGAATGAT
    GAAGACCTCC TGCCAGATGA TGTGGTACAG TATTTAAATT CCCAGAACCA AACAGGGTAT
    GGGCAGCAGT TGCAGAGTGG CATCTCTGAA GACAGCAAAG TAGCCCATGA GCCAGAGGAC
    TTGGACTTAG CAGGGCTGCC AGACAGTCAT GTTGGCCAGG AGTACCCAGC TCTGGAGCAG
    CCCTGCTCTG AGGGCAGTAA AACTGATTTG CCCATCCAGT GGAACGAGGT CAGTTCTGGA
    ACTTCTGATC TGTCATCCTC CAAGCTGAAG TGTGGTCAGC AGCGCCCTAG CGCACAGCAG
    CCTCGGGGTT TTGGGCTATA CAACAATATG GTGGTACACC CACATAATCT GTGGAAGGTT
    GGCACTGGCC CAGCTGGGGG ATATCAGACC CTCGGGGAGA ATAGCAGTAC CTACAATGGC
    CCAGAGCACT TTGCAATCCA CAGTGGGGAT GGACTTGGCA CCAATGGAAA TACTTTCCAT
    GAACAGCCCT TTAAGACCCA GCAGTATGGG AGCCAGCTCA ACAGGCAGCC ACTGACTTCC
    AGTGCTCTAG ATCATGCCTG TGGTACAGGG ATTCAAGGGT CCAAGCTAAA AGGCAACAGC
    TTGCAAGAGA ATGGGGGTTT GCTAGATTTC AGCCTGTCCG TGGCACCAAA TGAGTTAGCT
    GGCAACACAG TGAATGGCAT GCAAACCCAA GATCAAATGG GACAGGGATA CATTGCCCAT
    CAGCTACTCA GTGGCAGCAT GCAACACCAG GGGCCCAGCC GCCCTGGTCA ACAGGTACTA
    GGGCAGGTTG GTGCTACCTC ACATATCAAC ATCTATCAAG GGACAGAGAG CTGCCTACCA
    GGGACTCAGG ACAACAGCAG CCAGCCATCA AGCATGGCAG CTATCAGGGG CTACCAGCCC
    TGTGCCAGCT ATGGGGGTAA CAGGCGTCAG GCAATGCCAA GGGGCAACCT CACTCTGCAA
    CAAGGACAGC TCAGTGACAT GAGTCAGAGC AGCAGGGTGA ACAGCATCAA AATGGAGGCA
    CAAGGTCAGT CCCAGCAGCT CTGCTCTACC GTGCAGAATT ATTCCGGTCA GTTCTATGAC
    CAAACCATGG GCTTCAGTCA GCAAGACAGG AAAGCTGGCT CGTTCTCCCT CTCAGATGCC
    AACTGCCTGC TCCAAGGGAA TGGCACTGAA AACTCTGAGT TACTCTCCCC AGGTGCTAAC
    CAGGTAACAA GCACAGTTGA CAGCTTTGAG AGTCATGACC TAGAAGGTGT GCAGATTGAT
    TTTGATGCCA TCATAGATGA TGGGGACCAT ACCAGCCTAA TGTCAGGGGC CTTGAGCCCA
    AGTATTATTC AGAACCTTTC CCACAGCTCC TCCCGTCTCA CCACTCCGCG GGCATCCCTC
    CCATTCCCAT CCCTATCCAT GGGCACCACC AACATGGCTA TCGGGGATAT GAGTTCTTTG
    CTGACCTCCC TTGCAGAAGA AAGCAAGTTC CTTGCAGTTA TGCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21819
    Clone ID Related Accession (Same CDS sequence) NM_008130.2 , NM_008130.3
    Accession Version NM_008130.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-30
    Organism Mus musculus(house mouse)
    Product transcriptional activator GLI3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158261.1, X95255.1, CA752350.1 and AC173210.3. On Jan 5, 2007 this sequence version replaced NM_008130.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X95255.1, BC141135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
    GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG
    CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT
    GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC
    TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT
    GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT
    AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT
    ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA
    GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG
    ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT
    GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC
    AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA
    GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT
    GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT
    CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC
    ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG
    AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG
    TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT
    GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA
    GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA
    AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT
    ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT
    TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC
    ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC
    GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT
    GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA
    CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC
    CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG
    AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC
    AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA
    GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG
    GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG
    CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC
    AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC
    CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA
    GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC
    CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA
    CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA
    GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT
    GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC
    AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG
    GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA
    AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC
    CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG
    GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA
    AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC
    AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC
    CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC
    CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT
    CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA
    CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG
    GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT
    GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG
    CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA
    GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG
    TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA
    ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC
    AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT
    CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG
    GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG
    CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG
    GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG
    AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT
    GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT
    GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT
    GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC
    CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT
    CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG
    GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA
    GTTATGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032156.2
    CDS432..5183
    Translation

    Target ORF information:

    RefSeq Version NM_008130.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
    GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG
    CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT
    GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC
    TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT
    GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT
    AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT
    ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA
    GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG
    ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT
    GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC
    AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA
    GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT
    GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT
    CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC
    ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG
    AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG
    TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT
    GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA
    GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA
    AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT
    ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT
    TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC
    ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC
    GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT
    GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA
    CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC
    CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG
    AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC
    AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA
    GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG
    GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG
    CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC
    AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC
    CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA
    GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC
    CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA
    CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA
    GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT
    GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC
    AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG
    GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA
    AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC
    CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG
    GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA
    AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC
    AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC
    CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC
    CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT
    CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA
    CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG
    GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT
    GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG
    CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA
    GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG
    TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA
    ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC
    AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT
    CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG
    GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG
    CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG
    GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG
    AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT
    GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT
    GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT
    GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC
    CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT
    CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG
    GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA
    GTTATGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21819
    Clone ID Related Accession (Same CDS sequence) NM_008130.2 , NM_008130.3
    Accession Version NM_008130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Mus musculus(house mouse)
    Product transcriptional activator GLI3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163610.4, AC173115.2 and AC173210.3. On Aug 14, 2018 this sequence version replaced NM_008130.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC141135.1, X95255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
    GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG
    CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT
    GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC
    TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT
    GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT
    AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT
    ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA
    GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG
    ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT
    GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC
    AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA
    GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT
    GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT
    CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC
    ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG
    AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG
    TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT
    GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA
    GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA
    AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT
    ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT
    TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC
    ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC
    GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT
    GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA
    CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC
    CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG
    AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC
    AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA
    GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG
    GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG
    CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC
    AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC
    CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA
    GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC
    CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA
    CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA
    GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT
    GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC
    AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG
    GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA
    AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC
    CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG
    GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA
    AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC
    AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC
    CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC
    CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT
    CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA
    CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG
    GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT
    GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG
    CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA
    GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG
    TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA
    ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC
    AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT
    CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG
    GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG
    CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG
    GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG
    AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT
    GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT
    GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT
    GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC
    CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT
    CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG
    GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA
    GTTATGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032156.2
    CDS431..5182
    Translation

    Target ORF information:

    RefSeq Version NM_008130.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus GLI-Kruppel family member GLI3 (Gli3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
    GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG
    CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT
    GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC
    TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT
    GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT
    AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT
    ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA
    GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG
    ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT
    GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC
    AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA
    GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT
    GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT
    CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC
    ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG
    AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGAGCAAGCC CACAAGCGAG
    TCTGCAGTGA GCAGCACTGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCTGAT
    GAAGACCTCC CCAGCCCAGG GTCACGGGGC CAGCAGGAAC AGCCGGAAGG AACAACCCTA
    GTCAAGGAGG AAGCGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTACGAGACA
    AACTGCCACT GGGAAGGCTG CACCAGAGAG TTCGACACCC AAGATCAGCT TGTGCATCAT
    ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTCG TGTGCCGCTG GCTTGATTGT
    TCACGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTGCATAT GAGAAGACAC
    ACTGGGGAGA AGCCTCACAA ATGTACATTT GAAGGTTGCA CAAAAGCCTA CTCAAGACTC
    GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATACGT CTGTGAGCAT
    GAGGGCTGCA ACAAGGCTTT CTCTAATGCT TCAGATCGGG CCAAGCACCA AAACAGAACA
    CATTCCAATG AGAAACCGTA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC
    CCCAGCTCTC TCCGGAAACA CGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG
    AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CCCCTGGCCG GCCAACTCAG GGAGCATTTG GTGAGCAGAA GGAGCTGAGC
    AACACTACCT CAAAGCGGGA AGAGTGCCTC CAGGTGAAGA CTGTCAAGGC TGAGAAGCCC
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TGTAGCAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CATCAAACTT
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA
    GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CCAATAACAA CTATAGCTCG
    GGTGGGCCCG GGACCCTCCT CCCGAGCAGA AGTGACCTGT CAGGTGTAGA CTTCACAGTG
    CTGAACACAC TCAACAGGAG AGACAGCAAT ACCAGCACTA TCAGCTCTGC CTACCTGAGC
    AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG CGAGGCGTCC
    CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCTGACT CCTACGATCC CATCTCCACA
    GATGCTTCAA GGAGGTCCAG CGAGGCCAGC CAGGGTGACG GGCTGCCCAG TCTGCTCAGC
    CTCACACCCG TGCAGCAGTA CCGCCTTAAG GCCAAGTATG CAGCTGCCAC AGGTGGCCCA
    CCACCCACAC CTCTGCCCCA CATGGAGAGG CTGAGCCTGA AGACCAAGAT GGCCTTGCTA
    GGGGAAGGGA GGGACTCTGG GGTGACCCTG CCTCCAGTCC ATCCTCCTCG AAGATGCAGT
    GATGGGGGAG GTCACACATA CAGAGGGCGT CACCTGATGC CTCATGATGC ACTAGCGAAC
    AGTGTGAGGA GAGCCAGCGA CCCTGTGAGG ACAGTCTCGG AGAACATGTC ACTTGCCAGG
    GTTCAACGCT TCAGCAGCCT CAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCGGTGGAA
    AAGCGTAGCT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCAGCCAACC CCGGTACTTC
    CAGGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC TCTGACCATG
    GATGCTGATG CTAACTTGAA TGATGAAGAC CTCCTGCCAG ATGATGTGGT ACAGTATTTA
    AATTCCCAGA ACCAAACAGG GTATGGGCAG CAGTTGCAGA GTGGCATCTC TGAAGACAGC
    AAAGTAGCCC ATGAGCCAGA GGACTTGGAC TTAGCAGGGC TGCCAGACAG TCATGTTGGC
    CAGGAGTACC CAGCTCTGGA GCAGCCCTGC TCTGAGGGCA GTAAAACTGA TTTGCCCATC
    CAGTGGAACG AGGTCAGTTC TGGAACTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT
    CAGCAGCGCC CTAGCGCACA GCAGCCTCGG GGTTTTGGGC TATACAACAA TATGGTGGTA
    CACCCACATA ATCTGTGGAA GGTTGGCACT GGCCCAGCTG GGGGATATCA GACCCTCGGG
    GAGAATAGCA GTACCTACAA TGGCCCAGAG CACTTTGCAA TCCACAGTGG GGATGGACTT
    GGCACCAATG GAAATACTTT CCATGAACAG CCCTTTAAGA CCCAGCAGTA TGGGAGCCAG
    CTCAACAGGC AGCCACTGAC TTCCAGTGCT CTAGATCATG CCTGTGGTAC AGGGATTCAA
    GGGTCCAAGC TAAAAGGCAA CAGCTTGCAA GAGAATGGGG GTTTGCTAGA TTTCAGCCTG
    TCCGTGGCAC CAAATGAGTT AGCTGGCAAC ACAGTGAATG GCATGCAAAC CCAAGATCAA
    ATGGGACAGG GATACATTGC CCATCAGCTA CTCAGTGGCA GCATGCAACA CCAGGGGCCC
    AGCCGCCCTG GTCAACAGGT ACTAGGGCAG GTTGGTGCTA CCTCACATAT CAACATCTAT
    CAAGGGACAG AGAGCTGCCT ACCAGGGACT CAGGACAACA GCAGCCAGCC ATCAAGCATG
    GCAGCTATCA GGGGCTACCA GCCCTGTGCC AGCTATGGGG GTAACAGGCG TCAGGCAATG
    CCAAGGGGCA ACCTCACTCT GCAACAAGGA CAGCTCAGTG ACATGAGTCA GAGCAGCAGG
    GTGAACAGCA TCAAAATGGA GGCACAAGGT CAGTCCCAGC AGCTCTGCTC TACCGTGCAG
    AATTATTCCG GTCAGTTCTA TGACCAAACC ATGGGCTTCA GTCAGCAAGA CAGGAAAGCT
    GGCTCGTTCT CCCTCTCAGA TGCCAACTGC CTGCTCCAAG GGAATGGCAC TGAAAACTCT
    GAGTTACTCT CCCCAGGTGC TAACCAGGTA ACAAGCACAG TTGACAGCTT TGAGAGTCAT
    GACCTAGAAG GTGTGCAGAT TGATTTTGAT GCCATCATAG ATGATGGGGA CCATACCAGC
    CTAATGTCAG GGGCCTTGAG CCCAAGTATT ATTCAGAACC TTTCCCACAG CTCCTCCCGT
    CTCACCACTC CGCGGGCATC CCTCCCATTC CCATCCCTAT CCATGGGCAC CACCAACATG
    GCTATCGGGG ATATGAGTTC TTTGCTGACC TCCCTTGCAG AAGAAAGCAA GTTCCTTGCA
    GTTATGCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu48321
    Clone ID Related Accession (Same CDS sequence) XM_006516552.3
    Accession Version XM_006516552.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
    GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG
    CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT
    GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC
    TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT
    GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT
    AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT
    ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA
    GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG
    ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT
    GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC
    AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA
    GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT
    GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT
    CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC
    ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG
    AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGCAGAGCAA GCCCACAAGC
    GAGTCTGCAG TGAGCAGCAC TGGTGACCCT ATGCATAATA AGCGGTCCAA GATCAAGCCT
    GATGAAGACC TCCCCAGCCC AGGGTCACGG GGCCAGCAGG AACAGCCGGA AGGAACAACC
    CTAGTCAAGG AGGAAGCGGA CAAAGATGAA AGCAAGCAGG AGCCTGAAGT CATCTACGAG
    ACAAACTGCC ACTGGGAAGG CTGCACCAGA GAGTTCGACA CCCAAGATCA GCTTGTGCAT
    CATATCAATA ATGACCACAT TCATGGAGAA AAGAAGGAGT TCGTGTGCCG CTGGCTTGAT
    TGTTCACGAG AGCAGAAACC GTTCAAAGCC CAGTACATGT TGGTAGTGCA TATGAGAAGA
    CACACTGGGG AGAAGCCTCA CAAATGTACA TTTGAAGGTT GCACAAAAGC CTACTCAAGA
    CTCGAAAACC TGAAAACCCA CTTGAGATCT CACACTGGAG AGAAGCCATA CGTCTGTGAG
    CATGAGGGCT GCAACAAGGC TTTCTCTAAT GCTTCAGATC GGGCCAAGCA CCAAAACAGA
    ACACATTCCA ATGAGAAACC GTATGTATGC AAAATCCCTG GCTGCACCAA GCGTTACACA
    GACCCCAGCT CTCTCCGGAA ACACGTGAAG ACTGTGCATG GCCCTGAGGC TCATGTTACC
    AAGAAGCAGC GTGGGGACAT GCACCCTCGG CCTCCACCCC CGAGAGATTC CGGCAGCCAT
    TCACAGTCCA GGTCCCCTGG CCGGCCAACT CAGGGAGCAT TTGGTGAGCA GAAGGAGCTG
    AGCAACACTA CCTCAAAGCG GGAAGAGTGC CTCCAGGTGA AGACTGTCAA GGCTGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CTGATGGAGG TAGTGTAGCA
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC GGCAACCACA
    GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACATCAAA
    CTTGAAAGGC TAAAACAAGT GAATGGAATG TTTCCAAGAC TGAACCCTAT TCTACCCTCC
    AAAGCCCCTG CGGTATCTCC TCTCATAGGA AATGGCACAC AGTCCAATAA CAACTATAGC
    TCGGGTGGGC CCGGGACCCT CCTCCCGAGC AGAAGTGACC TGTCAGGTGT AGACTTCACA
    GTGCTGAACA CACTCAACAG GAGAGACAGC AATACCAGCA CTATCAGCTC TGCCTACCTG
    AGCAGCCGCA GATCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCAGGTC CAGCGAGGCG
    TCCCAGGCTG AAGGGCGACC CCAGAATGTG AGTGTGGCTG ACTCCTACGA TCCCATCTCC
    ACAGATGCTT CAAGGAGGTC CAGCGAGGCC AGCCAGGGTG ACGGGCTGCC CAGTCTGCTC
    AGCCTCACAC CCGTGCAGCA GTACCGCCTT AAGGCCAAGT ATGCAGCTGC CACAGGTGGC
    CCACCACCCA CACCTCTGCC CCACATGGAG AGGCTGAGCC TGAAGACCAA GATGGCCTTG
    CTAGGGGAAG GGAGGGACTC TGGGGTGACC CTGCCTCCAG TCCATCCTCC TCGAAGATGC
    AGTGATGGGG GAGGTCACAC ATACAGAGGG CGTCACCTGA TGCCTCATGA TGCACTAGCG
    AACAGTGTGA GGAGAGCCAG CGACCCTGTG AGGACAGTCT CGGAGAACAT GTCACTTGCC
    AGGGTTCAAC GCTTCAGCAG CCTCAATAGC TTTAATCCTC CAAATTTGCC TCCATCGGTG
    GAAAAGCGTA GCTTAGTGCT GCAAAATTAT ACCCGGCAGG AGAGCAGCCA ACCCCGGTAC
    TTCCAGGCAT CCCCTTGCCC TCCCAGCATC ACAGAGAATG TTGCCCTGGA GGCTCTGACC
    ATGGATGCTG ATGCTAACTT GAATGATGAA GACCTCCTGC CAGATGATGT GGTACAGTAT
    TTAAATTCCC AGAACCAAAC AGGGTATGGG CAGCAGTTGC AGAGTGGCAT CTCTGAAGAC
    AGCAAAGTAG CCCATGAGCC AGAGGACTTG GACTTAGCAG GGCTGCCAGA CAGTCATGTT
    GGCCAGGAGT ACCCAGCTCT GGAGCAGCCC TGCTCTGAGG GCAGTAAAAC TGATTTGCCC
    ATCCAGTGGA ACGAGGTCAG TTCTGGAACT TCTGATCTGT CATCCTCCAA GCTGAAGTGT
    GGTCAGCAGC GCCCTAGCGC ACAGCAGCCT CGGGGTTTTG GGCTATACAA CAATATGGTG
    GTACACCCAC ATAATCTGTG GAAGGTTGGC ACTGGCCCAG CTGGGGGATA TCAGACCCTC
    GGGGAGAATA GCAGTACCTA CAATGGCCCA GAGCACTTTG CAATCCACAG TGGGGATGGA
    CTTGGCACCA ATGGAAATAC TTTCCATGAA CAGCCCTTTA AGACCCAGCA GTATGGGAGC
    CAGCTCAACA GGCAGCCACT GACTTCCAGT GCTCTAGATC ATGCCTGTGG TACAGGGATT
    CAAGGGTCCA AGCTAAAAGG CAACAGCTTG CAAGAGAATG GGGGTTTGCT AGATTTCAGC
    CTGTCCGTGG CACCAAATGA GTTAGCTGGC AACACAGTGA ATGGCATGCA AACCCAAGAT
    CAAATGGGAC AGGGATACAT TGCCCATCAG CTACTCAGTG GCAGCATGCA ACACCAGGGG
    CCCAGCCGCC CTGGTCAACA GGTACTAGGG CAGGTTGGTG CTACCTCACA TATCAACATC
    TATCAAGGGA CAGAGAGCTG CCTACCAGGG ACTCAGGACA ACAGCAGCCA GCCATCAAGC
    ATGGCAGCTA TCAGGGGCTA CCAGCCCTGT GCCAGCTATG GGGGTAACAG GCGTCAGGCA
    ATGCCAAGGG GCAACCTCAC TCTGCAACAA GGACAGCTCA GTGACATGAG TCAGAGCAGC
    AGGGTGAACA GCATCAAAAT GGAGGCACAA GGTCAGTCCC AGCAGCTCTG CTCTACCGTG
    CAGAATTATT CCGGTCAGTT CTATGACCAA ACCATGGGCT TCAGTCAGCA AGACAGGAAA
    GCTGGCTCGT TCTCCCTCTC AGATGCCAAC TGCCTGCTCC AAGGGAATGG CACTGAAAAC
    TCTGAGTTAC TCTCCCCAGG TGCTAACCAG GTAACAAGCA CAGTTGACAG CTTTGAGAGT
    CATGACCTAG AAGGTGTGCA GATTGATTTT GATGCCATCA TAGATGATGG GGACCATACC
    AGCCTAATGT CAGGGGCCTT GAGCCCAAGT ATTATTCAGA ACCTTTCCCA CAGCTCCTCC
    CGTCTCACCA CTCCGCGGGC ATCCCTCCCA TTCCCATCCC TATCCATGGG CACCACCAAC
    ATGGCTATCG GGGATATGAG TTCTTTGCTG ACCTCCCTTG CAGAAGAAAG CAAGTTCCTT
    GCAGTTATGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516615.1
    CDS130..4884
    Translation

    Target ORF information:

    RefSeq Version XM_006516552.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus GLI-Kruppel family member GLI3 (Gli3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516552.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT 
    GGGAAATGTC CCACGAGAAC AGATGTCAGC GAGAAGGCCG TGGCCTCTAG TACCACTTCC
    AATGAGGATG AAAGTCCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG
    CAGCCTCAGA GTGTGCAGGG TCTCAACAAA ATCAGTGAGG AGCCCTCGAC GTCTAGTGAT
    GAGAGGGCCT CGCTGATCAA GAAAGAGATC CATGGCTCTC TACCACATCT GGCGGAGCCC
    TCTCTCCCTT ACCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGCTA CATGGAGCCT
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTATGATCCA TCTCCTATTC CTCCATTACA TGTGCCTTCT
    GCCTTATCTA GTAGCCCGAC GTATCCAGAC TTGCCCTTCA TTAGGATCTC CCCACACCGT
    AATCCCACTG CAGCCTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT
    ATGGACTACA TCCGCTCATT GCACAGCAGC CCATCCCTCT CCATGATCTC TGCTGCCCGA
    GGGCTGAGCC CTACAGATGC TCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG
    ATGGCTCTGC TGACAGGCCA GCGCAGCCCT TATGCAGACA TCCTTCCCTC AGCTGCCACT
    GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC
    AGCCCTAGGC TGTCAGCTAG GCCCAGCCGA AAGCGCACAC TGTCAATATC GCCACTGTCA
    GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCCC CTAACTCCTT GGTTACAATC
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCGGCAAGT
    GCAATCAGCC CTGCATTGAG CTTCACCTAC CCCTCCGCTC CTGTGTCTCT TCACATGCAT
    CAACAGATCC TAAGCCGACA GCAAAGCTTA GGCTCCGCAT TCGGACACAG CCCTCCTCTC
    ATCCACCCTG CTCCAACATT TCCAACACAG AGACCTATCC CTGGGATTCC GACAGTTCTG
    AACCCCGTCC AGGTCAGCTC TGGCCCTTCT GAGTCCTCAC AGCAGAGCAA GCCCACAAGC
    GAGTCTGCAG TGAGCAGCAC TGGTGACCCT ATGCATAATA AGCGGTCCAA GATCAAGCCT
    GATGAAGACC TCCCCAGCCC AGGGTCACGG GGCCAGCAGG AACAGCCGGA AGGAACAACC
    CTAGTCAAGG AGGAAGCGGA CAAAGATGAA AGCAAGCAGG AGCCTGAAGT CATCTACGAG
    ACAAACTGCC ACTGGGAAGG CTGCACCAGA GAGTTCGACA CCCAAGATCA GCTTGTGCAT
    CATATCAATA ATGACCACAT TCATGGAGAA AAGAAGGAGT TCGTGTGCCG CTGGCTTGAT
    TGTTCACGAG AGCAGAAACC GTTCAAAGCC CAGTACATGT TGGTAGTGCA TATGAGAAGA
    CACACTGGGG AGAAGCCTCA CAAATGTACA TTTGAAGGTT GCACAAAAGC CTACTCAAGA
    CTCGAAAACC TGAAAACCCA CTTGAGATCT CACACTGGAG AGAAGCCATA CGTCTGTGAG
    CATGAGGGCT GCAACAAGGC TTTCTCTAAT GCTTCAGATC GGGCCAAGCA CCAAAACAGA
    ACACATTCCA ATGAGAAACC GTATGTATGC AAAATCCCTG GCTGCACCAA GCGTTACACA
    GACCCCAGCT CTCTCCGGAA ACACGTGAAG ACTGTGCATG GCCCTGAGGC TCATGTTACC
    AAGAAGCAGC GTGGGGACAT GCACCCTCGG CCTCCACCCC CGAGAGATTC CGGCAGCCAT
    TCACAGTCCA GGTCCCCTGG CCGGCCAACT CAGGGAGCAT TTGGTGAGCA GAAGGAGCTG
    AGCAACACTA CCTCAAAGCG GGAAGAGTGC CTCCAGGTGA AGACTGTCAA GGCTGAGAAG
    CCCATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CTGATGGAGG TAGTGTAGCA
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCGA CCATTTCCAC GGCAACCACA
    GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACATCAAA
    CTTGAAAGGC TAAAACAAGT GAATGGAATG TTTCCAAGAC TGAACCCTAT TCTACCCTCC
    AAAGCCCCTG CGGTATCTCC TCTCATAGGA AATGGCACAC AGTCCAATAA CAACTATAGC
    TCGGGTGGGC CCGGGACCCT CCTCCCGAGC AGAAGTGACC TGTCAGGTGT AGACTTCACA
    GTGCTGAACA CACTCAACAG GAGAGACAGC AATACCAGCA CTATCAGCTC TGCCTACCTG
    AGCAGCCGCA GATCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCAGGTC CAGCGAGGCG
    TCCCAGGCTG AAGGGCGACC CCAGAATGTG AGTGTGGCTG ACTCCTACGA TCCCATCTCC
    ACAGATGCTT CAAGGAGGTC CAGCGAGGCC AGCCAGGGTG ACGGGCTGCC CAGTCTGCTC
    AGCCTCACAC CCGTGCAGCA GTACCGCCTT AAGGCCAAGT ATGCAGCTGC CACAGGTGGC
    CCACCACCCA CACCTCTGCC CCACATGGAG AGGCTGAGCC TGAAGACCAA GATGGCCTTG
    CTAGGGGAAG GGAGGGACTC TGGGGTGACC CTGCCTCCAG TCCATCCTCC TCGAAGATGC
    AGTGATGGGG GAGGTCACAC ATACAGAGGG CGTCACCTGA TGCCTCATGA TGCACTAGCG
    AACAGTGTGA GGAGAGCCAG CGACCCTGTG AGGACAGTCT CGGAGAACAT GTCACTTGCC
    AGGGTTCAAC GCTTCAGCAG CCTCAATAGC TTTAATCCTC CAAATTTGCC TCCATCGGTG
    GAAAAGCGTA GCTTAGTGCT GCAAAATTAT ACCCGGCAGG AGAGCAGCCA ACCCCGGTAC
    TTCCAGGCAT CCCCTTGCCC TCCCAGCATC ACAGAGAATG TTGCCCTGGA GGCTCTGACC
    ATGGATGCTG ATGCTAACTT GAATGATGAA GACCTCCTGC CAGATGATGT GGTACAGTAT
    TTAAATTCCC AGAACCAAAC AGGGTATGGG CAGCAGTTGC AGAGTGGCAT CTCTGAAGAC
    AGCAAAGTAG CCCATGAGCC AGAGGACTTG GACTTAGCAG GGCTGCCAGA CAGTCATGTT
    GGCCAGGAGT ACCCAGCTCT GGAGCAGCCC TGCTCTGAGG GCAGTAAAAC TGATTTGCCC
    ATCCAGTGGA ACGAGGTCAG TTCTGGAACT TCTGATCTGT CATCCTCCAA GCTGAAGTGT
    GGTCAGCAGC GCCCTAGCGC ACAGCAGCCT CGGGGTTTTG GGCTATACAA CAATATGGTG
    GTACACCCAC ATAATCTGTG GAAGGTTGGC ACTGGCCCAG CTGGGGGATA TCAGACCCTC
    GGGGAGAATA GCAGTACCTA CAATGGCCCA GAGCACTTTG CAATCCACAG TGGGGATGGA
    CTTGGCACCA ATGGAAATAC TTTCCATGAA CAGCCCTTTA AGACCCAGCA GTATGGGAGC
    CAGCTCAACA GGCAGCCACT GACTTCCAGT GCTCTAGATC ATGCCTGTGG TACAGGGATT
    CAAGGGTCCA AGCTAAAAGG CAACAGCTTG CAAGAGAATG GGGGTTTGCT AGATTTCAGC
    CTGTCCGTGG CACCAAATGA GTTAGCTGGC AACACAGTGA ATGGCATGCA AACCCAAGAT
    CAAATGGGAC AGGGATACAT TGCCCATCAG CTACTCAGTG GCAGCATGCA ACACCAGGGG
    CCCAGCCGCC CTGGTCAACA GGTACTAGGG CAGGTTGGTG CTACCTCACA TATCAACATC
    TATCAAGGGA CAGAGAGCTG CCTACCAGGG ACTCAGGACA ACAGCAGCCA GCCATCAAGC
    ATGGCAGCTA TCAGGGGCTA CCAGCCCTGT GCCAGCTATG GGGGTAACAG GCGTCAGGCA
    ATGCCAAGGG GCAACCTCAC TCTGCAACAA GGACAGCTCA GTGACATGAG TCAGAGCAGC
    AGGGTGAACA GCATCAAAAT GGAGGCACAA GGTCAGTCCC AGCAGCTCTG CTCTACCGTG
    CAGAATTATT CCGGTCAGTT CTATGACCAA ACCATGGGCT TCAGTCAGCA AGACAGGAAA
    GCTGGCTCGT TCTCCCTCTC AGATGCCAAC TGCCTGCTCC AAGGGAATGG CACTGAAAAC
    TCTGAGTTAC TCTCCCCAGG TGCTAACCAG GTAACAAGCA CAGTTGACAG CTTTGAGAGT
    CATGACCTAG AAGGTGTGCA GATTGATTTT GATGCCATCA TAGATGATGG GGACCATACC
    AGCCTAATGT CAGGGGCCTT GAGCCCAAGT ATTATTCAGA ACCTTTCCCA CAGCTCCTCC
    CGTCTCACCA CTCCGCGGGC ATCCCTCCCA TTCCCATCCC TATCCATGGG CACCACCAAC
    ATGGCTATCG GGGATATGAG TTCTTTGCTG ACCTCCCTTG CAGAAGAAAG CAAGTTCCTT
    GCAGTTATGC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.